MED1

Summary

Gene Symbol: MED1
Description: mediator complex subunit 1
Alias: CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, mediator of RNA polymerase II transcription subunit 1, ARC205, PPAR-binding protein, PPARG binding protein, TR-interacting protein 2, TRIP-2, activator-recruited cofactor 205 kDa component, p53 regulatory protein RB18A, peroxisome proliferator-activated receptor-binding protein, thyroid hormone receptor-associated protein complex 220 kDa component, thyroid hormone receptor-associated protein complex component TRAP220, thyroid receptor interacting protein 2, vitamin D receptor-interacting protein 230 kD, vitamin D receptor-interacting protein complex component DRIP205
Species: human

Top Publications

  1. pmc The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220
    Melanie Stumpf
    Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 103:18504-9. 2006
  2. pmc Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
    J Llopis
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:4363-8. 2000
  3. ncbi The activation function-1 domain of Nur77/NR4A1 mediates trans-activation, cell specificity, and coactivator recruitment
    K D Senali Abayratna Wansa
    University of Queensland Centre for Molecular and Cellular Biology Institute for Molecular Bioscience, St Lucia, Queensland 4072, Australia
    J Biol Chem 277:33001-11. 2002
  4. pmc Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex
    J D Fondell
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 93:8329-33. 1996
  5. ncbi Isolation and characterization of PBP, a protein that interacts with peroxisome proliferator-activated receptor
    Y Zhu
    Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 272:25500-6. 1997
  6. pmc The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion
    C X Yuan
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:7939-44. 1998
  7. ncbi A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
    W Gu
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:97-108. 1999
  8. ncbi Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators
    M Ito
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:361-70. 1999
  9. ncbi Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Nature 398:824-8. 1999
  10. ncbi Composite co-activator ARC mediates chromatin-directed transcriptional activation
    A M Näär
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Nature 398:828-32. 1999

Research Grants

  1. Louis B Rice; Fiscal Year: 2014
  2. Adriana E Rosato; Fiscal Year: 2014
  3. Christopher Davies; Fiscal Year: 2016
  4. Shahriar Mobashery; Fiscal Year: 2016
  5. Novel Compounds Targeting Bacterial PBPs
    LUIGI XERRI; Fiscal Year: 2013
  6. PENICILLIN RESPONSE GENES IN DRUG-RESISTANT PNEUMOCOCCI
    Alexander Tomasz; Fiscal Year: 1999
  7. Antibiotic Resistance in the Oral Streptococci
    Jeffrey Rogers; Fiscal Year: 2003
  8. Thomas G Bernhardt; Fiscal Year: 2016
  9. STAPHYLOCOCCAL METHICILLIN RESISTANCE LOCUS
    Gordon Archer; Fiscal Year: 2009
  10. GORDON LEE ARCHER; Fiscal Year: 2014

Detail Information

Publications242 found, 100 shown here

  1. pmc The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220
    Melanie Stumpf
    Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Proc Natl Acad Sci U S A 103:18504-9. 2006
    ..Mediator subunit Med1/TRAP220 is a key component of Mediator originally found to associate with nuclear hormone receptors...
  2. pmc Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription
    J Llopis
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 97:4363-8. 2000
    ..Here we demonstrate that both peroxisome proliferator-activated receptor binding protein (PBP) and steroid receptor coactivator-1 (SRC-1) are required for ligand-dependent transcription of transiently ..
  3. ncbi The activation function-1 domain of Nur77/NR4A1 mediates trans-activation, cell specificity, and coactivator recruitment
    K D Senali Abayratna Wansa
    University of Queensland Centre for Molecular and Cellular Biology Institute for Molecular Bioscience, St Lucia, Queensland 4072, Australia
    J Biol Chem 277:33001-11. 2002
    ..Thus, the AF-1 domain plays a major role in Nur77-mediated transcriptional activation, cofactor recruitment, and intra- and intermolecular interactions...
  4. pmc Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex
    J D Fondell
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 93:8329-33. 1996
    ..These findings demonstrate the ligand-dependent in vivo formation of a transcriptionally active TR-multisubunit protein complex and suggest a role for TRAPs as positive coactivators for gene-specific transcriptional activation...
  5. ncbi Isolation and characterization of PBP, a protein that interacts with peroxisome proliferator-activated receptor
    Y Zhu
    Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 272:25500-6. 1997
    ..We now report the isolation of a cDNA encoding a 165-kDa PPARgamma-binding protein, designated PBP which also serves as a coactivator...
  6. pmc The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion
    C X Yuan
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:7939-44. 1998
    ..Both TRAP220 and TRAP100 contain LXXLL domains found in other nuclear receptor-interacting proteins and both appear to reside ..
  7. ncbi A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation
    W Gu
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:97-108. 1999
    ..homologs of other yeast transcriptional regulatory factors (SOH1 and NUT2), and, significantly, some components (TRAP220, TRAP170/hRGR1, and TRAP100) of a human thyroid hormone receptor-associated coactivator complex...
  8. ncbi Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators
    M Ito
    Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021, USA
    Mol Cell 3:361-70. 1999
    ..In parallel with ligand-dependent interactions of nuclear receptors with the TRAP220 subunit, p53 and VP16 activation domains interact directly with a newly cloned TRAP80 subunit...
  9. ncbi Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA
    Nature 398:824-8. 1999
    ..The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes...
  10. ncbi Composite co-activator ARC mediates chromatin-directed transcriptional activation
    A M Näär
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Nature 398:828-32. 1999
    ..Thus, ARC/DRIP is a large composite co-activator that belongs to a family of related cofactors and is targeted by different classes of activator to mediate transcriptional stimulation...
  11. pmc Amplification and overexpression of peroxisome proliferator-activated receptor binding protein (PBP/PPARBP) gene in breast cancer
    Y Zhu
    Department of Pathology, Northwestern University Medical School, Chicago, IL 60611 3008, USA
    Proc Natl Acad Sci U S A 96:10848-53. 1999
    Peroxisome proliferator-activated receptor binding protein (PBP), a nuclear receptor coactivator, interacts with estrogen receptor alpha (ERalpha) in the absence of estrogen...
  12. pmc Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins
    A B Hittelman
    Department of Microbiology and the Kaplan Comprehensive Cancer Center, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA
    EMBO J 18:5380-8. 1999
    ..In addition, we show that another member of the DRIP complex, DRIP205, interacts with the GR ligand binding domain in a hormone-dependent manner and facilitates GR transactivation in ..
  13. ncbi Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex
    D Burakov
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Division, Cornell University, Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 275:20928-34. 2000
    ..It is recruited to the receptor in response to ligand through specific interactions of one subunit, DRIP205. We present evidence that DRIP205 interacts with another member of the steroid receptor subfamily, estrogen ..
  14. ncbi The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs
    S Malik
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021, USA
    Mol Cell 5:753-60. 2000
    ....
  15. pmc The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro
    Yun Kyoung Kang
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 99:2642-7. 2002
    ..Studies with extracts from TRAP220(-/-) fibroblasts reveal that these interactions depend on TRAP220, a TRAP/Mediator subunit previously shown to ..
  16. ncbi Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis
    Kai Ge
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 417:563-7. 2002
    ..This complex interacts physically with several nuclear receptors through the TRAP220 subunit, and with diverse activators through other subunits...
  17. pmc Identification of a transcriptionally active peroxisome proliferator-activated receptor alpha -interacting cofactor complex in rat liver and characterization of PRIC285 as a coactivator
    Sailesh Surapureddi
    Department of Pathology, Northwestern University, The Feinberg School of Medicine, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 99:11836-41. 2002
    ..PRIC complex includes known coactivators or coactivator-binding proteins (CBP, SRC-1, PBP, PRIP, PIMT, TRAP100, SUR-2, and PGC-1), other proteins that have not previously been described in association with ..
  18. ncbi Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells
    Tetsuo Fujita
    Institute of Applied Biochemistry, University of Tsukuba, 1 1 1 Tenno Dai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 278:26704-14. 2003
    ..These results indicated that fully activated AF-1 induces the stimulation of breast cancer growth and that the ratio between AF-1 coactivators and corepressors plays a key role to prevent proliferation of tumor by tamoxifen...
  19. ncbi BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220
    Osamu Wada
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 0034, Japan
    Oncogene 23:6000-5. 2004
    ..HeLa S3 nuclear extracts, we isolated BRCT-associated complexes and identified one of the purified components as TRAP220. We then performed interaction studies in vivo (co-immunoprecipitation) and in vitro (glutathione S-transferase ..
  20. pmc Regulation of the human cyclin C gene via multiple vitamin D3-responsive regions in its promoter
    Lasse Sinkkonen
    Department of Biochemistry, University of Kuopio PO Box 1627, FIN 70211 Kuopio, Finland
    Nucleic Acids Res 33:2440-51. 2005
    ....
  21. ncbi Spatio-temporal activation of chromatin on the human CYP24 gene promoter in the presence of 1alpha,25-Dihydroxyvitamin D3
    Sami Väisänen
    Department of Biochemistry, University of Kuopio, FIN 70211 Kuopio, Finland
    J Mol Biol 350:65-77. 2005
    ....
  22. ncbi MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor
    Weiwei Chen
    Department of Microbiology, New York University Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA
    Mol Endocrinol 20:560-72. 2006
    The Mediator subunits MED14 and MED1 have been implicated in transcriptional regulation by the glucocorticoid receptor (GR) by acting through its activation functions 1 and 2...
  23. pmc Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation
    Pradeep K Pandey
    Department of Physiology and Biophysics, Robert Wood Johnson Medical School, UMDNJ, Piscataway, NJ 08854, USA
    Mol Cell Biol 25:10695-710. 2005
    ..b>TRAP220/Med1 is a key component of TRAP/Mediator that targets the complex to nuclear hormone receptors and other types of ..
  24. pmc Mediator links epigenetic silencing of neuronal gene expression with x-linked mental retardation
    Ning Ding
    Department of Molecular Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78245, USA
    Mol Cell 31:347-59. 2008
    ..These findings implicate Mediator in epigenetic restriction of neuronal gene expression to the nervous system and suggest a pathologic basis for MED12-associated XLMR involving impaired REST-dependent neuronal gene regulation...
  25. doi Histone crosstalk between H3S10ph and H4K16ac generates a histone code that mediates transcription elongation
    Alessio Zippo
    Dipartimento di Biologia Molecolare Università di Siena, Siena, Italy
    Cell 138:1122-36. 2009
    ..Thus, the single phosphorylation H3S10ph at the FOSL1 enhancer triggers a cascade of events which activate transcriptional elongation...
  26. pmc CDK8 is a positive regulator of transcriptional elongation within the serum response network
    Aaron J Donner
    Howard Hughes Medical Institute, The University of Colorado at Boulder, Boulder, Colorado, USA
    Nat Struct Mol Biol 17:194-201. 2010
    ..Furthermore, CDK8-Mediator specifically interacts with positive transcription elongation factor b. Thus, we have uncovered a role for CDK8 in transcriptional regulation that may contribute to its oncogenic effects...
  27. pmc Arginine and glutamate-rich 1 (ARGLU1) interacts with mediator subunit 1 (MED1) and is required for estrogen receptor-mediated gene transcription and breast cancer cell growth
    Dingxiao Zhang
    Department of Cancer and Cell Biology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA
    J Biol Chem 286:17746-54. 2011
    ..b>MED1 (Mediator subunit 1) is the key Mediator subunit that directly interacts with estrogen receptor to mediate its ..
  28. pmc The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes
    C Rachez
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell Biol 20:2718-26. 2000
    ..In this report, we characterize the nuclear receptor binding features of DRIP205, a key subunit of the DRIP complex, that interacts directly with VDR and thyroid hormone receptor in response to ..
  29. ncbi Phosphorylation of transcriptional coactivator peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP). Stimulation of transcriptional regulation by mitogen-activated protein kinase
    Parimal Misra
    Department of Pathology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611 3008, USA
    J Biol Chem 277:48745-54. 2002
    Peroxisome proliferator-activated receptor (PPAR)-binding protein (PBP) is an important coactivator for PPARgamma and other transcription factors...
  30. ncbi 2-Methylene-19-nor-(20S)-1,25-dihydroxyvitamin D3 potently stimulates gene-specific DNA binding of the vitamin D receptor in osteoblasts
    Hironori Yamamoto
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 278:31756-65. 2003
    ..and ChIP indicate that 2MD is also more potent in promoting interaction with RXR and the coactivators SRC-1 and DRIP205. Finally, protease digestion studies revealed a unique VDR conformation in the presence of 2MD...
  31. ncbi Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins
    Qian Wu
    Department of Biochemistry and Biophysics, Department of Veterinary Integrative Biosciences, Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77843, USA
    J Biol Chem 279:53602-12. 2004
    Vitamin D-interacting protein 205 (DRIP205) is a mediator complex protein that anchors the complex to the estrogen receptor (ER) and other nuclear receptors (NRs)...
  32. ncbi MED220/thyroid receptor-associated protein 220 functions as a transcriptional coactivator with Pit-1 and GATA-2 on the thyrotropin-beta promoter in thyrotropes
    David F Gordon
    Division of Endocrinology, Department of Medicine, University of Colorado Health Sciences Center Fitzsimons, Mail Stop 8106, P O Box 6511, Aurora, Colorado 80049, USA
    Mol Endocrinol 20:1073-89. 2006
    ..Thus, the TSHbeta gene is maximally activated by a combination of three thyrotrope transcription factors that act via both protein-DNA and protein-protein interactions...
  33. pmc Cooperative activity of cdk8 and GCN5L within Mediator directs tandem phosphoacetylation of histone H3
    Krista D Meyer
    Department of Chemistry and Biochemistry, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    EMBO J 27:1447-57. 2008
    ..Indeed our results suggest that T/G-Mediator directs early events-such as modification of chromatin templates-in transcriptional activation...
  34. pmc Cross-talk between HER2 and MED1 regulates tamoxifen resistance of human breast cancer cells
    Jiajun Cui
    Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Cancer Res 72:5625-34. 2012
    ..In this study, we report that the ER coactivator MED1 is a novel cross-talk point for the HER2 and ERα pathways...
  35. ncbi Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription
    Paul O Hassa
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Biol Chem 280:40450-64. 2005
    ..Thus, acetylation of PARP-1 by p300/CREB-binding protein plays an important regulatory role in NF-kappaB-dependent gene activation by enhancing its functional interaction with p300 and the Mediator complex...
  36. ncbi Distinct roles of the steroid receptor coactivator 1 and of MED1 in retinoid-induced transcription and cellular differentiation
    Sébastien Flajollet
    INSERM U459, FacultédeMédecine Henri Warembourg, Lille F 59045, France
    J Biol Chem 281:20338-48. 2006
    ..Unexpectedly, Med1/TRAP220/DRIP205-depleted cells exhibited an exacerbated response to retinoids, both in terms transcriptional responses and of ..
  37. ncbi p300 mediates functional synergism between AF-1 and AF-2 of estrogen receptor alpha and beta by interacting directly with the N-terminal A/B domains
    Y Kobayashi
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
    J Biol Chem 275:15645-51. 2000
    ..and thyroid hormone receptor-associated protein-220/(vitamin D3 receptor-interacting protein- 205-(TRAP220/DRIP205) on the AF-1 activity in terms of synergism with the AF-2 function...
  38. ncbi The TRAP/SMCC/Mediator complex and thyroid hormone receptor function
    M Ito
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA
    Trends Endocrinol Metab 12:127-34. 2001
    ..The TRAP220 subunit mediates ligand-dependent interactions of the complex with TR and other nuclear receptors; and genetic ..
  39. ncbi [A case of penicillin-resistant pneumococcal pneumonia and penicillin-binding proteins of the clinical isolates]
    H Shigeno
    Second Department of Internal Medicine, Medical College of Oita
    Kansenshogaku Zasshi 66:508-15. 1992
    ..8%). The CZX resistance was observed only in the present case. The detection of penicillin-binding protein (PBP) and binding affinity of beta-lactam antibiotics were studied using PCG sensitive strain, S...
  40. ncbi Phenotypes and genes of resistance of pneumococci to penicillin isolated from children
    V Kotevska
    Microbiology and Parasitology Institute, Medical Faculty, Ss Cyril and Methodius University, Skopje, R Macedonia
    Prilozi 30:143-54. 2009
    ..Penicillin resistance in pneumococci is due to the development of altered penicillin-binding proteins (PBPs) in the bacterial cell wall. PBPs are known as six different variants (PBP1a, 1b, 2x, 2a, 2b and 3)...
  41. ncbi The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli
    M Terrak
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6, B 4000 Sart Tilman Liège, Belgium
    Mol Microbiol 34:350-64. 1999
    The penicillin-binding protein (PBP) 1b of Escherichia coli catalyses the assembly of lipid-transported N-acetyl glucosaminyl-beta-1, 4-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelyl+ ++- (L)-D-alanyl-D-alanine ..
  42. ncbi 1,25-Dihydroxyvitamin D3 stimulates cyclic vitamin D receptor/retinoid X receptor DNA-binding, co-activator recruitment, and histone acetylation in intact osteoblasts
    Sungtae Kim
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Bone Miner Res 20:305-17. 2005
    ....
  43. doi Ceftaroline: a novel broad-spectrum cephalosporin with activity against meticillin-resistant Staphylococcus aureus
    George G Zhanel
    Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
    Drugs 69:809-31. 2009
    ..Ceftaroline has the ability to bind to penicillin-binding protein (PBP)2a, an MRSA-specific PBP that has low affinity for most other beta-lactam antibacterials...
  44. ncbi Peroxisome proliferator-activated receptors, coactivators, and downstream targets
    C Qi
    Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell Biochem Biophys 32:187-204. 2000
    ..system, the authors recently cloned mouse steroid receptor coactivator-1 (SRC-1) and PPAR-binding protein (PBP), and identified them as PPAR coactivators...
  45. ncbi Reactivity of penicillin-binding proteins with peptidoglycan-mimetic beta-lactams: what's wrong with these enzymes?
    Helen R Josephine
    Department of Chemistry, Wesleyan University, Middletown, Connecticut 06459, USA
    Biochemistry 45:15873-83. 2006
    ..kinetics of their inhibition of a panel of penicillin-binding proteins spanning the major classes (Escherichia coli PBP 2 and PBP 5, Streptococcus pneumoniae PBP 1b, PBP 2x and PBP 3, the Actinomadura R39 DD-peptidase, and the ..
  46. pmc Target for bacteriostatic and bactericidal activities of beta-lactam antibiotics against Escherichia coli resides in different penicillin-binding proteins
    G Satta
    Istituto di Microbiologia, Universita Cattolica del Sacro Cuore, Rome, Italy
    Antimicrob Agents Chemother 39:812-8. 1995
    The relationship between cell-killing kinetics and penicillin-binding protein (PBP) saturation has been evaluated in the permeability mutant Escherichia coli DC2 in which the antimicrobial activity of beta-lactams has been described as ..
  47. ncbi Synthetic progestins stimulate prostatic binding protein messenger RNAs in the rat ventral prostate
    C Labrie
    Medical Research Council Group in Molecular Endocrinology, Laval University Medical Center, Quebec, Canada
    Mol Cell Endocrinol 68:169-79. 1990
    ..markers of androgen action in the rat ventral prostate, namely the levels of messenger RNAs encoding the C1 (PBP-C1) and C3 (PBP-C3) components of rat prostatic binding protein (PBP)...
  48. pmc Rapid detection of the mosaic structure of the Neisseria gonorrhoeae penA Gene, which is associated with decreased susceptibilities to oral cephalosporins
    Susumu Ochiai
    Advanced Medicine Development Department, R and D and Business Development Segment, Mitsubishi Chemical Medience Corporation, 3 30 1 Shimura, Itabashi ku, Tokyo 174 8555, Japan
    J Clin Microbiol 46:1804-10. 2008
    In Neisseria gonorrhoeae, the mosaic structure of the penA gene (encoding penicillin-binding protein 2 [PBP 2]), which is composed of fragments of the penA genes from Neisseria cinerea and Neisseria perflava, has been significantly ..
  49. pmc Analysis of penicillin-binding protein genes of clinical isolates of Streptococcus pneumoniae with reduced susceptibility to amoxicillin
    Mignon du Plessis
    Pneumococcal Diseases Research Unit of the Medical Research Council, National Health Laboratory Service and University of the Witwatersrand, de Korte Street, Hillbrow, PO Box 1038, Johannesburg 2000, South Africa
    Antimicrob Agents Chemother 46:2349-57. 2002
    ..resistance phenotype of higher amoxicillin MICs in relation to the penicillin MICs prompted an analysis of the pbp genes from three such strains isolated in France...
  50. doi Investigation of the mechanism of the cell wall DD-carboxypeptidase reaction of penicillin-binding protein 5 of Escherichia coli by quantum mechanics/molecular mechanics calculations
    Qicun Shi
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA
    J Am Chem Soc 130:9293-303. 2008
    Penicillin-binding protein 5 (PBP 5) of Escherichia coli hydrolyzes the terminal D-Ala-D-Ala peptide bond of the stem peptides of the cell wall peptidoglycan...
  51. pmc Complete sequences of six penicillin-binding protein genes from 40 Streptococcus pneumoniae clinical isolates collected in Japan
    Yumiko Sanbongi
    Pharmaceutical Research Department, Meiji Seika Kaisha Ltd, 760 Morooka cho, Kohoku Ku, Yokohama 222 8567, Japan
    Antimicrob Agents Chemother 48:2244-50. 2004
    All six penicillin-binding protein (PBP) genes, namely, pbp1a, pbp1b, pbp2a, pbp2b, pbp2x, and pbp3, of 40 Streptococcus pneumoniae clinical isolates, including penicillin-resistant S...
  52. ncbi Non-Penicillin-Binding protein mediated high-level penicillin and cephalosporin resistance in a Hungarian clone of Streptococcus pneumoniae
    A M Smith
    Department of Clinical Microbiology and Infectious Diseases, South African Institute for Medical Research, Johannesburg
    Microb Drug Resist 6:105-10. 2000
    ..The role that each penicillin-binding protein (PBP) plays in the development of resistance in these strains was investigated via transformation of susceptible strain ..
  53. pmc Association of amino acid substitutions in penicillin-binding protein 3 with beta-lactam resistance in beta-lactamase-negative ampicillin-resistant Haemophilus influenzae
    K Ubukata
    Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd, 760 Morookacho, Kohoku Ku, Yokohama, Japan
    Antimicrob Agents Chemother 45:1693-9. 2001
    The affinity of [(3)H]benzylpenicillin for penicillin-binding protein (PBP) 3A was reduced in 25 clinical isolates of beta-lactamase-negative ampicillin (AMP)-resistant (BLNAR) Haemophilus influenzae for which the AMP MIC was > or =1...
  54. ncbi [Meningitis due to streptococcus viridans]
    D Alba
    Departamento de Medicina Interna, Hospital La Paz, Paseo de la Castellana, Madrid
    Rev Neurol 23:656-60. 1995
    ..VS are normally sensitive to penicillin G. However VS may be resistant to penicillin (low affinity of PBP to penicillin) and cause serious infections, including meningitis...
  55. ncbi In vitro activity, beta-lactamase stability and PBP affinity of RU 51,746-2, the active metabolite of the new orally absorbed cephalosporin ester, RU 51807
    M Boaretti
    Istituto di Microbiologia, , Italy
    J Chemother 3:57-61. 1991
    ..RU 51,746-2 showed a good affinity for high molecular weight penicillin binding proteins (PBPs) of E. coli. PBP 3 was found to be the target for growth inhibition, whereas the additional saturation of PBP 1 and 2 was required ..
  56. pmc Diversity of beta-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae
    Henri Dabernat
    Laboratoire de Microbiologie, Centre national de reference des Haemophilus influenzae, Toulouse, France
    Antimicrob Agents Chemother 46:2208-18. 2002
    The sequences of the ftsI gene, encoding the transpeptidase domain of penicillin binding protein (PBP) 3A and/or PBP 3B, which are involved in septal peptidoglycan synthesis, were determined for 108 clinical strains of Haemophilus ..
  57. doi Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits
    Gaozhong Shen
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Biol Chem 283:7503-12. 2008
    ..Single and double mutant strains for cpcS-I and cpcU exhibited slower growth rates, reduced PBP levels, and impaired assembly of phycobilisomes, but a cpcV mutant had no discernable phenotype...
  58. pmc Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae
    Shuqing Zhao
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Antimicrob Agents Chemother 53:3744-51. 2009
    ..Ceph(i)] strains) contain a mosaic penA allele encoding penicillin-binding protein 2 (PBP 2) with nearly 60 amino acid differences compared to the sequence of wild-type PBP 2, together with a set of ..
  59. pmc Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM beta-lactamase/penicillin-binding protein 5 chimera
    Y H Chang
    Department of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125
    Proc Natl Acad Sci U S A 87:2823-7. 1990
    ..into a beta-lactamase background, we have prepared a chimeric protein by inserting a 28-amino acid segment of PBP-5 of Escherichia coli in place of the corresponding region of the RTEM-1 beta-lactamase...
  60. ncbi Alterations in penicillin binding protein gene of Streptococcus pneumoniae and their correlation with susceptibility patterns
    Yoshinobu Ohsaki
    First Department of Medicine, Asahikawa Medical College, 2 1 1 1 Midorigaoka Higashi, Asahikawa 078 8510, Hokkaido, Japan
    Int J Antimicrob Agents 22:140-6. 2003
    Penicillin binding protein (pbp) gene alterations of 328 clinical isolates of Streptococcus pneumoniae were examined for a correlation with their antibiotic-resistance...
  61. doi Single dose 1 g ceftriaxone for urogenital and pharyngeal infection caused by Neisseria gonorrhoeae
    Tetsuro Muratani
    Department of Urology, Asian Clinical Research Group, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan
    Int J Urol 15:837-42. 2008
    ..gonorrhoeae) including the oral cephem-resistant strain with chimera penicillin-binding protein 2 (PBP-2) (cefozopran-resistant N. gonorrhoeae, CZRNG).
  62. ncbi Evaluation of bactericidal activity of cefotaxime and other beta-lactams by a novel method
    G Satta
    Istituto di Microbiologia dell Università di Siena
    Drugs 35:35-40. 1988
    ..first evaluated the kinetics of cellular killing by various beta-lactams as related to penicillin-binding-protein (PBP) binding in Escherichia coli DC2, a hyperpermeable mutant. We concluded that in E...
  63. ncbi Continuous administration of PBP-2- and PBP-3-specific beta-lactams causes higher cytokine responses in murine Pseudomonas aeruginosa and Escherichia coli sepsis
    Jacqueline Buijs
    Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands
    J Antimicrob Chemother 59:926-33. 2007
    ..High-dose ceftazidime, which inhibits penicillin-binding protein (PBP)-1 in Gram-negative bacteria, causes quick bacteriolysis and low endotoxin release...
  64. ncbi Penicillin-binding protein 1B from Escherichia coli contains a membrane association site in addition to its transmembrane anchor
    R A Nicholas
    University of North Carolina, Department of Pharmacology, Chapel Hill 27599 7365
    J Biol Chem 268:5632-41. 1993
    A working structural model of penicillin-binding protein 1B (PBP 1B) from Escherichia coli derived from previous data consists of a highly charged aminoterminal cytoplasmic tail, a 23-amino-acid hydrophobic transmembrane anchor, and a ..
  65. pmc Association of a thr-371 substitution in a conserved amino acid motif of penicillin-binding protein 1A with penicillin resistance of Streptococcus pneumoniae
    Y Asahi
    Department of Clinical Pathology, Teikyo University School of Medicine, 2 11 1 Kaga, Itabashi ku, Tokyo 173 8605, Japan
    Antimicrob Agents Chemother 42:2267-73. 1998
    ..We determined the nucleotide sequence between 1,903 and 3,097 bp of pbp1a, which encodes the transpeptidase domain of PBP 1A, from clinical isolates of penicillin-resistant Streptococcus pneumoniae (PRSP) serotypes 19 (n = 8), 6 (n = 9), ..
  66. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    ..these proteins make to the physiology of Escherichia coli, we constructed 192 mutants from which eight PBP genes were deleted in every possible combination...
  67. pmc First characterization of heterogeneous resistance to imipenem in invasive nontypeable Haemophilus influenzae isolates
    Marina Cerquetti
    Department of Infectious, Parasitic and Immune Mediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
    Antimicrob Agents Chemother 51:3155-61. 2007
    ..The sequencing of the ftsI gene encoding penicillin-binding protein 3 (PBP 3) and comparison with the sequence of the imipenem-susceptible H...
  68. ncbi On the relationships between molecular conformation, affinity towards penicillin-binding proteins, and biological activity of penicillin G-sulfoxide
    F Beise
    Robert Koch Institute, Federal Health Organization, Berlin, Bundesrepublik Deutschland
    Z Naturforsch C 43:656-64. 1988
    The binding capacity of penicillin G-sulfoxide towards the penicillin-binding proteins (PBP) of Staphylococcus aureus H was studied...
  69. ncbi Investigation of the substrate spectrum of the human mismatch-specific DNA N-glycosylase MED1 (MBD4): fundamental role of the catalytic domain
    F Petronzelli
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    J Cell Physiol 185:473-80. 2000
    The human DNA repair protein MED1 (also known as MBD4) was isolated as an interactor of the mismatch repair protein MLH1 in a yeast two-hybrid screening...
  70. ncbi Presence of international multiresistant clones of Streptococcus pneumoniae in Colombia
    M C Vela
    Grupo de Microbiologia, Instituto Nacional de Salud, Bogota, Colombia
    Microb Drug Resist 7:153-64. 2001
    ..serotype 23F isolates were grouped in two clusters: 15 (31%) multiresistant isolates showed PFGE pattern A and PBP I profile, thus making them indistinguishable from Spain23F-1 clone, and 34 (69%) with PFGE pattern C, PBP II ..
  71. pmc Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity
    George Nicola
    Department of Biochemistry, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 49:8094-104. 2010
    ..We have determined crystal structures of Escherichia coli PBP 5 as covalent complexes with imipenem, cloxacillin, and cefoxitin...
  72. ncbi Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties
    R A Nicholas
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    J Biol Chem 263:2034-40. 1988
    ..protein 5 (sPBP 5) from Escherichia coli were produced in large amounts by placing the dacA gene that encodes PBP 5 under the control of the trp-lac fusion promoter...
  73. ncbi The mechanism of staphylococci resistance to methicillin: a critical analysis of dominant opinions
    G Satta
    Institute of Microbiology of the University of Siena, Italy
    J Chemother 3:144-8. 1991
    ..to methicillin has been reported to be constantly associated with production of a novel penicillin-binding protein (PBP), indicated as PBP2a...
  74. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
    The low-molecular-weight (LMW) penicillin-binding protein, PBP 5, plays a dominant role in determining the uniform cell shape of Escherichia coli...
  75. doi Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4
    Thomas B Clarke
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Biochemistry 48:2675-83. 2009
    Escherichia coli PBP4 is the archetypal class C, low molecular mass penicillin binding protein (LMM-PBP) and possesses both dd-carboxypeptidase and dd-endopeptidase activity...
  76. ncbi Mosaic pbpX genes of major clones of penicillin-resistant Streptococcus pneumoniae have evolved from pbpX genes of a penicillin-sensitive Streptococcus oralis
    C Sibold
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 12:1013-23. 1994
    Penicillin-resistant clinical isolates of Streptococcus pneumoniae contain mosaic penicillin-binding protein (PBP) genes that encode PBPs with decreased affinity for beta-lactam antibiotics...
  77. pmc Restoration of susceptibility of methicillin-resistant Staphylococcus aureus to beta-lactam antibiotics by acidic pH: role of penicillin-binding protein PBP 2a
    Sandrine Lemaire
    Unité de Pharmacologie Cellulaire et Moléculaire, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    J Biol Chem 283:12769-76. 2008
    ..The central factor for the MRSA phenotype is the function of the penicillin-binding protein (PBP) 2a, which maintains transpeptidase activity while being poorly inhibited by beta-lactams because of a closed ..
  78. pmc Discrete roles for peroxisome proliferator-activated receptor gamma and retinoid X receptor in recruiting nuclear receptor coactivators
    W Yang
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 20:8008-17. 2000
    ..A single DRIP subunit, DRIP205 (TRAP220, PBP), binds directly to PPARgamma...
  79. pmc Mecillinam resistance in Escherichia coli is conferred by loss of a second activity of the AroK protein
    D Vinella
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2785, USA
    J Bacteriol 178:3818-28. 1996
    Mecillinam, a beta-lactam antibiotic specific to penicillin-binding protein 2 (PBP 2) in Escherichia coli, blocks cell wall elongation and, indirectly, cell division, but its lethality can be overcome by increased levels of ppGpp, the ..
  80. pmc Highly variable penicillin resistance determinants PBP 2x, PBP 2b, and PBP 1a in isolates of two Streptococcus pneumoniae clonal groups, Poland 23F-16 and Poland 6B-20
    Radoslaw Izdebski
    National Medicines Institute, Division of Clinical Microbiology and Infection Prevention, Chełmska 30 34, Warsaw 00 725, Poland
    Antimicrob Agents Chemother 52:1021-7. 2008
    ..pneumoniae clonal groups that are prevalent in Poland, Poland 23F-16 and Poland 6B-20, were investigated by PBP profile analysis, antibody reactivity pattern analysis, and DNA sequence analysis of the transpeptidase (TP) domain-..
  81. doi Comparative genome analysis of high-level penicillin resistance in Streptococcus pneumoniae
    Amelia G Tait-Kamradt
    Antibacterial Biology, Pfizer Global Research and Development, Groton, Connecticut, USA
    Microb Drug Resist 15:69-75. 2009
    ..Previous studies have traced the changes in penicillin binding proteins (PBP) that result in decreased penicillin susceptibility, and the role of several PBP genes in high-level resistance...
  82. ncbi Use of a beta-lactamase fusion vector to investigate the organization of penicillin-binding protein 1B in the cytoplasmic membrane of Escherichia coli
    A Edelman
    Microbial Genetics Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK
    Mol Microbiol 1:101-6. 1987
    ..of TEM beta-lactamase was fused to random positions within the coding region of the penicillin-binding protein 1B (PBP 1B) gene and the nucleotide sequences across the fusion junctions of 100 in-frame fusions were determined...
  83. pmc Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202 9037, USA
    J Bacteriol 183:3055-64. 2001
    ..by investigating the effects of these proteins on the aberrant morphology of DeltadacA mutants, which produce no PBP 5...
  84. ncbi Crystal structure of the 47-kDa lipoprotein of Treponema pallidum reveals a novel penicillin-binding protein
    Ranjit K Deka
    Departments of Microbiology and Biochemistry, University of Texas Southwestern Medical Center, Dallas 75390, USA
    J Biol Chem 277:41857-64. 2002
    ..We previously identified the T. pallidum 47-kDa lipoprotein (Tp47) as a penicillin-binding protein (PBP)...
  85. ncbi Extract of Pelargonium sidoides (EPs 7630) improves phagocytosis, oxidative burst, and intracellular killing of human peripheral blood phagocytes in vitro
    Andreas Conrad
    Institute of Environmental Medicine and Hospital Epidemiology, University Medical Center Freiburg, Freiburg, Germany
    Phytomedicine 14:46-51. 2007
    ..objective of this study was to examine the impact of EPs 7630 on the activity of human peripheral blood phagocytes (PBP). A whole blood-based, flow cytometric assay was used to simultaneously assess phagocytosis and oxidative burst...
  86. pmc Nasopharyngeal Haemophilus influenzae carriage in Japanese children attending day-care centers
    Koichi Hashida
    Department of Otorhinolaryngology, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Yahatanishi ku, Kitakyushu 807 8555, Japan
    J Clin Microbiol 46:876-81. 2008
    ..and analyzed them by antimicrobial susceptibility testing, PCR for bla(TEM-1) and the penicillin-binding protein (PBP) gene, and pulsed-field gel electrophoresis (PFGE). The overall carriage rate was 47.4% (172/363), and 37...
  87. pmc Reconstruction of the phenotypes of methicillin-resistant Staphylococcus aureus by replacement of the staphylococcal cassette chromosome mec with a plasmid-borne copy of Staphylococcus sciuri pbpD gene
    Aude Antignac
    The Rockefeller University, New York, NY 10021, USA
    Antimicrob Agents Chemother 53:435-41. 2009
    ..carry a close homologue of mecA in the form of pbpD, the genetic determinant of penicillin binding protein 4 (PBP 4) of S. sciuri...
  88. pmc Induction of the major integral membrane protein of mouse liver peroxisomes by peroxisome proliferators
    N Chen
    Division of Science and Technology, Griffith University, Brisbane, Queensland, Australia
    Biochem J 283:605-10. 1992
    ..PMP68) by a number of these agents, and compared this with their effect on the peroxisomal bifunctional protein (PBP), an enzyme of the beta-oxidation pathway which is located in the peroxisome matrix...
  89. doi Concentration-dependency of beta-lactam-induced filament formation in Gram-negative bacteria
    J Buijs
    Department of Medical Microbiology and Infectious Diseases, Atrium Medical Centre, Heerlen, The Netherlands
    Clin Microbiol Infect 14:344-9. 2008
    Ceftazidime and cefotaxime are beta-lactam antibiotics with dose-related affinities for penicillin-binding protein (PBP)-3 and PBP-1. At low concentrations, these antibiotics inhibit PBP-3, leading to filament formation...
  90. ncbi RB18A, whose gene is localized on chromosome 17q12-q21.1, regulates in vivo p53 transactivating activity
    R Frade
    Immunochimie des Régulations Cellulaires et des Interactions Virales, Institut National de la Santé et de la Recherche Médicale INSERM U 354, Centre INSERM, H pital Saint Antoine, Paris, France
    Cancer Res 60:6585-9. 2000
    ..Drane et al., Oncogene, 15: 3013-3024, 1997) RB18A, a new gene whose encoded Mr 205,000 protein interacted in vitro, through its COOH-terminal domain, with p53...
  91. ncbi Kinetics of beta-lactam interactions with penicillin-susceptible and -resistant penicillin-binding protein 2x proteins from Streptococcus pneumoniae. Involvement of acylation and deacylation in beta-lactam resistance
    W P Lu
    Anti infective Research, Procter and Gamble Pharmaceuticals, Health Care Research Center, Mason, Ohio 45040, USA
    J Biol Chem 276:31494-501. 2001
    ..The millimolar range K(d) implies that the beta-lactam fits to the active site pocket of the penicillin-sensitive PBP rather poorly, whereas the extremely fast k(2) value indicates that this step contributes most of the binding ..
  92. ncbi Diversity of penicillin-nonsusceptible Streptococcus pneumoniae circulating in Iceland after the introduction of penicillin-resistant clone Spain(6B)-2
    Raquel Sá-Leão
    Laboratory of Microbiology, Rockefeller University, New York, New York 10021, USA
    J Infect Dis 186:966-75. 2002
    ..typing; determination of pbp1a, pbp2b, and pbp2x gene restriction patterns; and partial sequencing of these pbp genes...
  93. ncbi Cloning and genomic localization of the murine LPS-induced CXC chemokine (LIX) gene, Scyb5
    Jeffrey B Smith
    Pediatrics Neonatology B2 325, MDCC, David Geffen School of Medicine and Mattel Children s Hospital at UCLA, 10833 Le Conte Avenue, Los Angeles, CA 90095 1752, USA
    Immunogenetics 54:599-603. 2002
    ..The sequence and mapping data suggest that the human ENA78-PBP-PF4 and GCP2- psi PBP-PF4V1 loci arose from an evolutionarily recent duplication of an ancestral locus related to ..
  94. ncbi Parallel evolution of ligand specificity between LacI/GalR family repressors and periplasmic sugar-binding proteins
    Kaoru Fukami-Kobayashi
    Center for Information Biology and DDBJ, National Institute of Genetics, Mishima, Japan
    Mol Biol Evol 20:267-77. 2003
    ..family evolved by acquiring the DNA-binding domain in the N-terminal of an ancestral periplasmic binding protein (PBP)...
  95. pmc Role of class A penicillin-binding proteins in PBP5-mediated beta-lactam resistance in Enterococcus faecalis
    Ana Arbeloa
    INSERM E0004 LRMA, Universite Paris VI, 75270 Paris, France
    J Bacteriol 186:1221-8. 2004
    Peptidoglycan polymerization complexes contain multimodular penicillin-binding proteins (PBP) of classes A and B that associate a conserved C-terminal transpeptidase module to an N-terminal glycosyltransferase or morphogenesis module, ..
  96. pmc Amino acid mutations essential to production of an altered PBP 2X conferring high-level beta-lactam resistance in a clinical isolate of Streptococcus pneumoniae
    Anthony M Smith
    Respiratory and Meningeal Pathogens Research Unit, National Institute for Communicable Diseases, Medical Research Council, Johannesburg, South Africa
    Antimicrob Agents Chemother 49:4622-7. 2005
    Altered penicillin-binding protein 2X (PBP 2X) is a primary beta-lactam antibiotic resistance determinant and is essential to the development of penicillin and cephalosporin resistance in the pneumococcus...
  97. pmc Regulation of the human p21(waf1/cip1) gene promoter via multiple binding sites for p53 and the vitamin D3 receptor
    Anna Saramaki
    Department of Biochemistry, University of Kuopio, FIN 70211 Kuopio, Finland
    Nucleic Acids Res 34:543-54. 2006
    ..promoter regions by monitoring simultaneous occupancy of VDR with the co-activator proteins CBP, SRC-1 and TRAP220. Taken together, we demonstrated that the human p21((waf1/cip1)) gene is a primary 1alpha,25(OH)2D3-responding ..
  98. ncbi Alterations of pbp1a, pbp2b, and pbp2x in Streptococcus pneumoniae isolates from children with otolaryngological infectious disease in the Sapporo district of Japan
    Atsushi Harimaya
    Department of Otolaryngology, Sapporo Medical University School of Medicine, S 1, W 16, Chuo Ku, Sapporo, Hokkaido 060 8543, Japan
    J Infect Chemother 12:366-71. 2006
    ..of beta-lactam susceptibility and polymerase chain reaction (PCR)-based genotyping of penicillin-binding proteins (PBP) 1A, 2B, and 2X were performed for Streptococcus pneumoniae isolates from children with otolaryngological ..
  99. ncbi Differential recruitment of coactivators to the vitamin D receptor transcriptional complex by 1alpha,25-dihydroxyvitamin D3 analogs
    Marie K Schwinn
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Arch Biochem Biophys 465:443-51. 2007
    ..With the exception of two of these coactivators, DRIP205 and DRIP240, all proteins were recruited to the transcriptional complex in a dose-dependent manner...
  100. doi Gastric cancer with high-level microsatellite instability: target gene mutations, clinicopathologic features, and long-term survival
    Mario Falchetti
    Department of Experimental Medicine, University La Sapienza, 00161 Rome, Italy
    Hum Pathol 39:925-32. 2008
    ..IGFIIR, RIZ, TCF4, DP2), apoptosis (BAX, BCL10, FAS, CASPASE5, APAF1), and DNA repair genes (hMSH6, hMSH3, MED1, RAD50, BLM, ATR, BRCA2, MRE11)...
  101. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
    ..However, PBP5 clearly contributes to maintenance of cell shape in some PBP mutant backgrounds...

Research Grants63

  1. Louis B Rice; Fiscal Year: 2014
    ..faecium is the expression of low affinity class B penicillin-binding protein Pbp5. As a class B Pbp with only transpeptidase activity, Pbp5 must coordinate its activities with that of glycosyltransferases to ..
  2. Adriana E Rosato; Fiscal Year: 2014
    ..to the acquisition of a gene (mecA) that encodes a ?-lactam insensitive target enzyme, penicillin-binding protein (PBP)2a...
  3. Christopher Davies; Fiscal Year: 2016
    ..gonorrhoeae involved in peptidoglycan synthesis. One is penicillin-binding protein 2 (PBP 2), a transpeptidase that forms peptide cross-links during the latter stages of cell wall synthesis, and the ..
  4. Shahriar Mobashery; Fiscal Year: 2016
    ..and two resistant determinants, a class A [unreadable]-lactamases and a special penicillin-binding protein (PBP) referred to as PBP2a...
  5. Novel Compounds Targeting Bacterial PBPs
    LUIGI XERRI; Fiscal Year: 2013
    ..identified a novel series of highly selective, non-cytotoxic small molecule lead compounds that bind to and disrupt PBP function in both gram-positive and gram-negative bacteria...
  6. PENICILLIN RESPONSE GENES IN DRUG-RESISTANT PNEUMOCOCCI
    Alexander Tomasz; Fiscal Year: 1999
    ..Resistant isolates exhibit a high degree of polymorphism in their PBPs and contain PBP genes of "mosaic" structure...
  7. Antibiotic Resistance in the Oral Streptococci
    Jeffrey Rogers; Fiscal Year: 2003
    ..pneumoniae quickly led to the hypothesis that these pbp genes evolved by recombination with homologs from other streptococcal species, in particular the oral streptococci...
  8. Thomas G Bernhardt; Fiscal Year: 2016
    ..beyond the "crutch" of antibiotic probes, we developed a genetic approach to identify factors needed for proper PBP function in vivo using E. coli as a model system. E. coli encodes three bi-functional PBPs: PBP1A, PBP1B, and PBP1C...
  9. STAPHYLOCOCCAL METHICILLIN RESISTANCE LOCUS
    Gordon Archer; Fiscal Year: 2009
    ..The gene responsible for methicillin resistance (MR), mecA, encodes a new PBP (PBP2a) that can maintain cell wall structure but is resistant to [unreadable]-lactam inhibition...
  10. GORDON LEE ARCHER; Fiscal Year: 2014
    ..The gene responsible for methicillin resistance (MR), mecA, encodes a new PBP (PBP2a) that can maintain cell wall structure but is resistant to beta-lactam inhibition mecA is carried on a ..
  11. Mechanistic studies on peptidoglycan tailoring that mediate biofilm stability
    MATTHEW DENNIS LEBAR; Fiscal Year: 2013
    ..We will also show that B. subtilis PBP 1a and 1b can perform this function in vitro by utilizing chemically synthesized lipid II substrate and cloned ..
  12. METHICILLIN RESISTANCE OF STAPHYLOCOCCUS AUREUS
    Henry Chambers; Fiscal Year: 1993
    ..Methicillin resistant strains of staphylococci produced a novel 78kd penicillin-binding protein (PBP), PBP 2a, that has low binding affinity for beta-lactam antibiotics...
  13. Peptidomimetics of D-Ala-D-Ala as Novel Antibacterials
    Norma Dunlap; Fiscal Year: 2003
    ..Penicillins are bacterial cell wall synthesis inhibitors and act by inhibition of Penicillin Binding Proteins (PBP's), also know as D-D-peptidases...
  14. THE PENICILLIN-BINDING PROTEINS OF BACILLUS SUBTILIS
    CHRISTINE BUCHANAN; Fiscal Year: 1993
    ..A major effort will be made to evaluate the PBP changes that occur during sporulation because there are 2 penicillin-sensitive steps in this developmental process ..
  15. NEW INHIBITORS FOR THE PENICILLIN BINDING PROTEINS
    WILLIAM GUTHEIL; Fiscal Year: 2002
    ..In preliminary studies precise and sensitive UV-vis based coupled enzyme assays have been established for PBP activity, for both D-Ala and D-Lac based substrates, and used to partially characterize the activity of E...
  16. RELATING STRUCTURE/FUNCTION IN E. COLI PENICILLIN-PBPS
    Robert Rosenberg; Fiscal Year: 1993
    ..are: 1) to continue to supply protein for the solving of the crystal structure of a soluble form of a mutant PBP 5 (in collaboration with Dr...
  17. Regulation/mecA gene expression/clinic S.aureus isolates
    Adriana Rosato; Fiscal Year: 2005
    ..by acquiring a gene (mecA) that encodes a beta-lactam insensitive target enzyme, penicillin binding protein (PBP)2a...
  18. RESISTANCE TO BETA-LACTAM ANTIBIOTICS
    Shahriar Mobashery; Fiscal Year: 2010
    ..both beta- lactamases, enzymes that hydrolytically destroy these antibiotics, or a penicillin-binding protein (PBP 2a) that is not readily inhibited by beta-lactam antibiotics. Three specific Aims are proposed...
  19. REGULATION OF BACTERIAL CELL DIVISION
    James Walker; Fiscal Year: 1980
    ..sep has been cloned in a viable phage vector. This hybrid phage and direct assays of sep mutants were used to demonstrate that the sep product is a penicillin-binding protein (PBP-3).
  20. Bayar Thimmapaya; Fiscal Year: 2016
    ..In this context, transcription coactivator Med1 (also known as PBP/TRAP220/DRIP205), a key subunit of the mammalian Mediator complex, interacts with nuclear hormone receptors and transcription ..
  21. DHAN V KALVAKOLANU; Fiscal Year: 2014
    ..Studies during the last funding period also identified Med1, a major subunit of Mediator, an IFN-induced binding partner of C/EBP- 2...
  22. Henry F Chambers; Fiscal Year: 2016
    ..of resistance to ?-lactams that is independent of penicillinase and the low affinity penicillin bindin protein (PBP), PBP2a, the two known mechanisms of ?-lactam resistance in Staphylococcus aureus...
  23. Redesign of the Carbapenem Scaffold
    Bret M Benton; Fiscal Year: 2012
    ..In addition, novel carbapenems possessing good antibacterial activity will be profiled for PBP binding potency and susceptibility to renal dehydropeptidases along with an in vitro DMPK investigation of ..
  24. PENICILLIN INTERACTIVE PROTEINS OF STAPHYLOCOCCUS AUREUS
    Henry Chambers; Fiscal Year: 2003
    ..Resistance is mainly due to production a novel low affinity penicillin bind protein, PBP 2a, a well wall synthetic enzyme. PBP 2a seems to substitute for all other PBPs...
  25. BIOSYNTHESIS OF BACTERIAL CELL WALL COMPONENTS
    Francis Neuhaus; Fiscal Year: 1991
    ..To support this hypothesis, it is our aim to isolate the amidase from this organism and relate this enzyme to PBP-6, the primary in vivo target of penicillin...
  26. Folate Status, Genomic Uracil, and the Balance of Base Excision Repair Activity
    Michael D Wyatt; Fiscal Year: 2011
    ..There is an additional link that is yet to be explored. MBD4 (MED1) specifically removes uracil or thymine when paired opposite guanine in CpG sequences...
  27. Alfonso Bellacosa; Fiscal Year: 2014
    ..The overall goal of this project is to understand how two related mammalian base excision repair enzymes, MED1 and TDG, maintain genomic and epigenomic stability at CpG sites, by preventing both mutations and altered ..
  28. Robert G Roeder; Fiscal Year: 2015
    ..The cofactors of special interest include the primary receptor-interacting subunit of th Mediator (MED1), factors that may provide alternative or redundant pathways for Mediator recruitment, the brown fat ..
  29. Vitamin D Hormone: Function and Mechanism of Action
    SYLVIA S CHRISTAKOS; Fiscal Year: 2010
    ..in the previous grant period related to the factors that affect 24(OH)ase transcription (C/EBP (3, CBP, YY1 and DRIP205)...
  30. NR1D1 pathway in breast cancer
    Douglas S Conklin; Fiscal Year: 2012
    ..Two of these genes, NR1D1 and PBP, map closely to the ERBB2 locus and are commonly found on amplicons that are responsible for ERBB2 overexpression...
  31. Transcriptional Coactivators in Liver Function
    Janardan K Reddy; Fiscal Year: 2013
    ..These include SRC- 1, PBP/MED1, PRIP (NCoA6), PRIP-interacting protein PIMT (NCoA6IP), PRIC285, and PRIC320 among others...
  32. DANIEL DAVID BIKLE; Fiscal Year: 2014
    ..Moreover, we have found a specific role for DRIP205 in the regulation of wnt signaling pathways regulating keratinocyte proliferation, whereas SRC3 uniquely ..
  33. MOLECULAR STRUCTURE OF STEROIDS
    William Duax; Fiscal Year: 2001
    ..microbial cholesterol esterase (Cc ChE) of relevance to atherosclerosis, and a mammalian prostatic binding protein (PBP) implicated in prostate cancer therapy...
  34. ROLE OF THE MED1 SUBUNIT OF MEDIATOR CO-ACTIVATOR COMPLEX IN NEURAL STEM CELLS
    Narendrakumar Ramanan; Fiscal Year: 2013
    ..In this pilot project we'll investigate the function of the Mediator1 subunit (Med1) in neural stem cells using conditional Med1 knockout mice...
  35. MED1 MUTATIONS IN COLORECTAL CANCER
    Alfonso Bellacosa; Fiscal Year: 2002
    ..In a yeast two-hybrid screening with the mismatch repair protein MLH1 as "bait", we cloned MED1, a novel human DNA repair gene...
  36. ANDROGEN-RESPONSIVE PROSTATE EPITHELIAL CELLS
    WALLACE MC KEEHAN; Fiscal Year: 1980
    An androgen-dependent, prostate-specific protein (Prostatic Binding Protein; PBP) was isolated from rat ventral prostate cytosol and antiserum against it was prepared in rabbits...
  37. NUCLEAR RECEPTOR COACTIVATORS AND BREAST CANCER
    Yijun Zhu; Fiscal Year: 2004
    ..The proposed research focuses on the role of PBP, a peroxisome proliferator activated receptor--binding protein, in breast development and neoplastic conversion. Dr...
  38. NEW HUMAN DNA REPAIR ENDONUCLEASE
    Alfonso Bellacosa; Fiscal Year: 2002
    ..By employing the yeast interaction trap with hMLH1 as bait , MED1 (mismatch repair endonuclease1), a novel human gene encoding a protein with homology to bacterial endonucleases, ..
  39. Preschool Language & Social Curriculum Evaluation
    JANIS KUPERSMIDT; Fiscal Year: 2002
    ..The proposed curriculum will expand the Preschool Behavior Project (PBP) that integrates activities promoting language/preliteracy skills with topics related to socioemotional development...
  40. THYROTROPIN BETA SUBUNIT GENE TRANSCRIPTION FACTORS
    ELI RIDGWAY; Fiscal Year: 2006
    ..An additional factor, TRAP220, is a non-DNA binding coactivator/mediator...
  41. TRANSCRIPTION COFACTORS OF THE THYROID HORMONE RECEPTOR
    Joseph Fondell; Fiscal Year: 2007
    ..A single subunit, TRAP220, acts as a pivotal signaling mediator of cellular growth and development in that it targets TRAP/Mediator to NRs ..
  42. Vascular PPAR alpha and atherosclerosis
    Jayme Borensztajn; Fiscal Year: 2009
    ..The nuclear receptor co-activator PBP (PPAR- Binding Protein) is essential for PPARa - regulated gene expression and its absence in hepatocytes causes an ..
  43. COACTIVATORS IN BREAST DEVELOPMENT AND TUMORIGENESIS
    Yijun Zhu; Fiscal Year: 2009
    ..Peroxisome proliferator-activated receptor (PPAR) binding protein (PBP, also known as TRAP220/DRIP205/MED1) is one of the coactivators we isolated a few years ago...
  44. Transcriptional Coactivators in Liver Function
    Janardan Reddy; Fiscal Year: 2007
    ..Using PPAR as bait in a yeast two-hybrid screen, we cloned and characterized PBP and PRIP as nuclear receptor coactivators, and PIMT as PRIP-binding protein...
  45. STRUCTURE-BASED DESIGN OF PROTEIN FUNCTION
    Homme Hellinga; Fiscal Year: 2006
    ..Using these design techniques, several members of the E. coli periplasmic binding protein (PBP) superfamily that normally bind sugars or amino acids have been converted into receptors that recognize chemically ..
  46. REGULATION OF INTESTINAL CALCIUM TRANSPORT BY VITAMIN D
    Daniel Bikle; Fiscal Year: 2001
    ..These manipulations will all be tested for their ability to block 1,25D-stimulated calcium transport. ..
  47. VITAMIN D AND THE DIFFERENTIATION OF KERATINOCYTES
    Daniel Bikle; Fiscal Year: 2003
    ..abstract_text> ..
  48. A Comprehensive Analysis of Genetic Variation in DNA Repair Genes in Relation to
    Christopher Haiman; Fiscal Year: 2007
    ..The ability to accurately identify high-risk sub-groups may have profound implications on endometrial cancer prevention as well as advance research aimed at targeted drug development. [unreadable] [unreadable] [unreadable]..
  49. GLUCOCORTICOID ATTENUATED RESPONSE GENES IN INFLAMMATION
    Jeffrey Smith; Fiscal Year: 2001
    ..Genes identified in this search are likely to encode pro-inflammatory mediators that participate in a wide range of inflammatory lung diseases in addition to endotoxemia. ..
  50. Mechanisms of antibiotic action in Neisseria gonorrhoeae.
    Robert A Nicholas; Fiscal Year: 2010
    ..we describe a proteomics approach to identify and characterize proteins that bind to penicillin-binding protein 1 (PBP 1), PBP 2, and the lytic transglycosylase MltA, and thus reveal the form and function of the multi-enzyme complexes ..
  51. Trafficking/signaling of P2Y receptors in polarized epithelial cells
    Robert A Nicholas; Fiscal Year: 2011
    ..Our studies will provide novel and important information on the cellular mechanisms of P2Y receptor targeting and the regulation of P2Y receptor signaling activity in epithelial cells. ..
  52. Fine-mapping of 8q24 and Loci Identified in Genome-Wide Studies for Breast & Colo
    CHRISTOPHER ALAN HAIMAN; Fiscal Year: 2010
    ..We expect findings from this work to guide the development of future preventive, early detection and prognostic strategies ..
  53. RATIONAL DESIGN OF ANTISICKLING AGENTS
    MARTIN SAFO; Fiscal Year: 2002
    ....
  54. Effects of Psychological Stress on the Stratum Corneum
    Kenneth Feingold; Fiscal Year: 2008
    ..Aim 4: To determine if the abnormalities in permeability barrier homeostasis and SC integrity/ cohesion induced by GC treatment in humans, are due to the inhibition of epidermal lipid synthesis. ..
  55. HNF-4 and coactivators in apoAl gene regulation
    Sohail Malik; Fiscal Year: 2008
    ..They will also potentially reveal targets for future therapeutic developments in the metabolic and cardiovascular areas. [unreadable] [unreadable]..
  56. Stratum Corneum Acidification in the Neonate
    Kenneth Feingold; Fiscal Year: 2007
    ..4) To determine if activators of PPAR alpha, PPAR delta, and/or LXR accelerate the formation of an acidic SC, the mechanism for this acceleration of acidification, and the functional consequences of such an acceleration. ..
  57. Structure of Beta-lactam resistance regulators
    MARTIN SAFO; Fiscal Year: 2007
    ..Subsequently, the structures of the proteins will be determined using X-ray crystallography. [unreadable] [unreadable]..
  58. Rational Design of Novel Estrogen Receptor Antagonists
    MARTIN SAFO; Fiscal Year: 2007
    ..A long-range goal of this proposal is to generate and optimize antiestrogens that are "pure" alpha-receptor antagonists. ..
  59. PPARs and LXR Regulate Epidermal Differentiation
    Kenneth Feingold; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  60. Role of penicillin binding protein 1a in GBS virulence
    Amanda Jones; Fiscal Year: 2006
    ..abstract_text> ..
  61. Structure and Function Analysis of TRAP220
    Wei Chen; Fiscal Year: 2005
    ..The most important subunit of this complex for nuclear receptor function is TRAP220, since it binds to nuclear receptors in a ligand-dependent manner and thus anchors the entire complex to promoter-..
  62. DNA MISMATCH REPAIR--LESION REPAIR AND STRAND TARGETING
    James Drummond; Fiscal Year: 2004
    ..We will test this model by asking whether free DNA ends, or defined gaps within circular DNAs, are capable of directing MR to the discontinuous strand. ..
  63. Gordon Research Conference on Staphylococcal Diseases
    BRIGITTE BERGER BACHI; Fiscal Year: 2003
    ..S. This will afford a unique and rich opportunity for fellowship and scientific exchange between young and established investigators in the field. ..