lysozyme

Summary

Gene Symbol: lysozyme
Description: lysozyme
Alias: LYZF1, LZM, lysozyme C, 1,4-beta-N-acetylmuramidase C, c-type lysozyme, lysozyme F1
Species: human

Top Publications

  1. ncbi Strategies for new antimicrobial proteins and peptides: lysozyme and aprotinin as model molecules
    Hisham R Ibrahim
    Department of Biochemistry and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima 890 0065, Japan
    Curr Pharm Des 8:671-93. 2002
  2. ncbi Refinement of human lysozyme at 1.5 A resolution analysis of non-bonded and hydrogen-bond interactions
    P J Artymiuk
    J Mol Biol 152:737-62. 1981
  3. ncbi Lateral interaction between electrostatically adsorbed and covalently immobilized proteins on the surface of cation-exchange sorbents
    C K Ratnayake
    PerSeptive Biosystems Inc, Framingham, MA 01701, USA
    J Chromatogr A 743:25-32. 1996
  4. ncbi Cyclodextrin aided separation of peptides and proteins by capillary zone electrophoresis
    A S Rathore
    Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA
    J Chromatogr A 796:367-73. 1998
  5. pmc Human lactoferrin but not lysozyme neutralizes HSV-1 and inhibits HSV-1 replication and cell-to-cell spread
    Hannamari Valimaa
    Institute of Dentistry, University of Turku, Turku, Finland
    Virol J 6:53. 2009
  6. ncbi Myeloid Elf-1-like factor, an ETS transcription factor, up-regulates lysozyme transcription in epithelial cells through interaction with promyelocytic leukemia protein
    Mary Ann Suico
    Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
    J Biol Chem 279:19091-8. 2004
  7. ncbi Human lysozyme gene mutations cause hereditary systemic amyloidosis
    M B Pepys
    Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    Nature 362:553-7. 1993
  8. pmc 'Fragile' liver and massive hepatic haemorrhage due to hereditary amyloidosis
    R F Harrison
    Department of Pathology, University of Birmingham
    Gut 38:151-2. 1996
  9. ncbi Hereditary renal amyloidosis associated with variant lysozyme in a large English family
    J D Gillmore
    Immunological Medicine Unit, Division of Medicine, ICSM, Hammersmith Hospital, London, UK
    Nephrol Dial Transplant 14:2639-44. 1999
  10. ncbi Local cooperativity in the unfolding of an amyloidogenic variant of human lysozyme
    Denis Canet
    Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QH, UK
    Nat Struct Biol 9:308-15. 2002

Research Grants

  1. Recombinant Antibodies from the Sea Lamprey
    ZEEV contact PANCER; Fiscal Year: 2010
  2. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James E Casanova; Fiscal Year: 2013
  3. Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
    NEIL DANIELSON; Fiscal Year: 2009
  4. MOLECULAR STUDIES OF SANFILIPPO SYNDROME TYPE B
    ELIZABETH NEUFELD; Fiscal Year: 2010
  5. Molecular Engineering of Humanized Anti-Staphlococcal Lytic Enzymes
    Karl E Griswold; Fiscal Year: 2012
  6. Epithelial PLUNC as a determinant of Airway Mucosal Antimicrobial Activity
    YUANPU PETER DI; Fiscal Year: 2012
  7. Crystallization and structure determination of the mu-opioid receptor
    Roger K Sunahara; Fiscal Year: 2012
  8. Vadim Cherezov; Fiscal Year: 2014
  9. Lysozymes and immunity in Anopheles gambiae
    Susan M Paskewitz; Fiscal Year: 2010
  10. Dan Feng; Fiscal Year: 2014

Detail Information

Publications497 found, 100 shown here

  1. ncbi Strategies for new antimicrobial proteins and peptides: lysozyme and aprotinin as model molecules
    Hisham R Ibrahim
    Department of Biochemistry and Biotechnology, Faculty of Agriculture, Kagoshima University, Kagoshima 890 0065, Japan
    Curr Pharm Des 8:671-93. 2002
    ..b>Lysozyme, muramidase, and aprotinin, a protease inhibitor, both exhibit antimicrobial activities against different ..
  2. ncbi Refinement of human lysozyme at 1.5 A resolution analysis of non-bonded and hydrogen-bond interactions
    P J Artymiuk
    J Mol Biol 152:737-62. 1981
  3. ncbi Lateral interaction between electrostatically adsorbed and covalently immobilized proteins on the surface of cation-exchange sorbents
    C K Ratnayake
    PerSeptive Biosystems Inc, Framingham, MA 01701, USA
    J Chromatogr A 743:25-32. 1996
    ..The extent of interaction was also influenced by concentration of the immobilized protein in the case of lysozyme. Interactions between the analyte and immobilized protein were found to occur between both the same two proteins ..
  4. ncbi Cyclodextrin aided separation of peptides and proteins by capillary zone electrophoresis
    A S Rathore
    Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA
    J Chromatogr A 796:367-73. 1998
    ..using raw fused-silica capillaries in CZE of the four standard proteins: alpha-chymotrypsinogen A, cytochrome c, lysozyme and ribonuclease A...
  5. pmc Human lactoferrin but not lysozyme neutralizes HSV-1 and inhibits HSV-1 replication and cell-to-cell spread
    Hannamari Valimaa
    Institute of Dentistry, University of Turku, Turku, Finland
    Virol J 6:53. 2009
    ..In the current study, we investigated the anti-HSV-1 activity of human lactoferrin (hLf) and lysozyme (hLz), two highly cationic polypeptides of the mucosal innate defence system...
  6. ncbi Myeloid Elf-1-like factor, an ETS transcription factor, up-regulates lysozyme transcription in epithelial cells through interaction with promyelocytic leukemia protein
    Mary Ann Suico
    Department of Molecular Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
    J Biol Chem 279:19091-8. 2004
    ..MEF) or Elf4, which is a member of the ETS transcription factor family, up-regulates the basal expression of lysozyme gene in epithelial cells and is constitutively localized in the nucleus...
  7. ncbi Human lysozyme gene mutations cause hereditary systemic amyloidosis
    M B Pepys
    Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK
    Nature 362:553-7. 1993
    ..b>Lysozyme is a ubiquitous bacteriolytic enzyme present in external secretions and in polymorphs and macrophages, but its ..
  8. pmc 'Fragile' liver and massive hepatic haemorrhage due to hereditary amyloidosis
    R F Harrison
    Department of Pathology, University of Birmingham
    Gut 38:151-2. 1996
    ..liver showed a surprisingly modest amount of amyloid, which was shown immunohistochemically to be derived from lysozyme, and a striking absence of reticulin staining...
  9. ncbi Hereditary renal amyloidosis associated with variant lysozyme in a large English family
    J D Gillmore
    Immunological Medicine Unit, Division of Medicine, ICSM, Hammersmith Hospital, London, UK
    Nephrol Dial Transplant 14:2639-44. 1999
    Two kindreds with hereditary systemic amyloidosis caused by the first two mutations to be described in the human lysozyme gene were discovered recently and study of the variant lysozyme has been powerfully informative about mechanisms of ..
  10. ncbi Local cooperativity in the unfolding of an amyloidogenic variant of human lysozyme
    Denis Canet
    Oxford Centre for Molecular Sciences, New Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QH, UK
    Nat Struct Biol 9:308-15. 2002
    ..exchange experiments monitored by NMR and mass spectrometry reveal that the amyloidogenic D67H mutation in human lysozyme significantly reduces the stability of the beta-domain and the adjacent C-helix in the native structure...
  11. ncbi Formation of amyloid aggregates from human lysozyme and its disease-associated variants using hydrostatic pressure
    Fernanda G De Felice
    Departamento de Bioquimica Medica, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21944 590, Brazil
    FASEB J 18:1099-101. 2004
    Formation of amyloid deposits from the Ile56Thr or Asp67His variants of human lysozyme is a hallmark of autosomal hereditary systemic amyloidosis...
  12. ncbi Reduced global cooperativity is a common feature underlying the amyloidogenicity of pathogenic lysozyme mutations
    Mireille Dumoulin
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 346:773-88. 2005
    ..is associated with the deposition in vital organs of full-length mutational variants of the antibacterial protein lysozyme. Here, we report experimental data that permit a detailed comparison to be made of the behaviour of two of these ..
  13. ncbi Rationalising lysozyme amyloidosis: insights from the structure and solution dynamics of T70N lysozyme
    Russell J K Johnson
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    J Mol Biol 352:823-36. 2005
    T70N human lysozyme is the only known naturally occurring destabilised lysozyme variant that has not been detected in amyloid deposits in human patients...
  14. ncbi ALys amyloidosis caused by compound heterozygosity in exon 2 (Thr70Asn) and exon 4 (Trp112Arg) of the lysozyme gene
    Christoph Rocken
    Institute of Pathology, Otto von Guericke University, Magdeburg, Germany
    Hum Mutat 27:119-20. 2006
    ..T70N) and exon 4 (p.W112R) of the lysozyme gene (LYZ), with both mutations being present on the same allele...
  15. ncbi The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species
    Janet R Kumita
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK
    J Mol Biol 369:157-67. 2007
    ..of the extracellular molecular chaperone, clusterin, on the in vitro aggregation of mutational variants of human lysozyme, including one associated with familial amyloid disease...
  16. ncbi The human lysozyme gene. Sequence organization and chromosomal localization
    C W Peters
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany
    Eur J Biochem 182:507-16. 1989
    ..two overlapping recombinant lambda-phage clones from a genomic lambda-EMBL3 library containing 25 kb of the human lysozyme gene region. Furthermore a full-lenght human lysozyme cDNA clone of 1...
  17. ncbi Hereditary renal amyloidosis caused by a new variant lysozyme W64R in a French family
    Sophie Valleix
    Laboratoire de Biochimie et Biologie Moleculaire, Hopital Cochin, Assistance Publique Hôpitaux de Paris AP HP et INSERM EMI 00 05, Paris, France
    Kidney Int 61:907-12. 2002
    ..Five proteins--transthyretin, fibrinogen alpha-A chain, apolipoprotein AI, lysozyme, apolipoprotein AII, cystatin C and gelsolin--can be associated with hereditary amyloidosis involving the kidney.
  18. ncbi A novel lysozyme mutation Phe57Ile associated with hereditary renal amyloidosis
    Masahide Yazaki
    Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Kidney Int 63:1652-7. 2003
    Variant forms of lysozyme, a ubiquitous bacteriolytic enzyme, are known to lead to hereditary non-neuropathic renal amyloidosis and, so far, three different mutations of the lysozyme gene have been reported...
  19. ncbi Oxidative refolding of amyloidogenic variants of human lysozyme
    Rachel Wain
    Oxford Centre for Molecular Sciences and Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK
    J Mol Biol 351:662-71. 2005
    The oxidative refolding of human lysozyme and its two best characterised amyloidogenic variants, Ile56Thr and Asp67His, has been investigated in vitro by means of the concerted application of a range of biophysical techniques...
  20. ncbi Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris
    Janet R Kumita
    Department of Chemistry, University of Cambridge, UK
    FEBS J 273:711-20. 2006
    We report the secreted expression by Pichia pastoris of two human lysozyme variants F57I and W64R, associated with systemic amyloid disease, and describe their characterization by biophysical methods...
  21. ncbi Structural and folding dynamic properties of the T70N variant of human lysozyme
    Gennaro Esposito
    Dipartimento di Scienze e Tecnologie Biomediche, Universita di Udine, 33100 Udine, Italy
    J Biol Chem 278:25910-8. 2003
    ..improved by the biochemical and biophysical studies carried out on the two amyloidogenic variants of human lysozyme, I56T and D67H...
  22. ncbi Hyaline droplet accumulation in rodent kidney proximal tubules: an association with histiocytic sarcoma
    G C Hard
    MRC Toxicology Unit, Carshalton, Surrey, United Kingdom
    Toxicol Pathol 19:88-97. 1991
    ..on 11 cases of rat and 8 cases of mouse histiocytic sarcoma, the protein in hyaline droplets was identified as lysozyme, a known major secretory product of monocytes and macrophages...
  23. ncbi Removal of endotoxins by affinity sorbents
    F B Anspach
    GBF-National Research Centre for Biotechnology, Braunschweig, Germany
    J Chromatogr A 711:81-92. 1995
    ..Endotoxin removal from protein solutions depended on the net charge of the desired protein. Lysozyme as a model for positively charged proteins enhanced endotoxin removal...
  24. ncbi Purification of protein and recycling of polymers in a new aqueous two-phase system using two thermoseparating polymers
    J Persson
    Department of Biochemistry, Lund University, Sweden
    J Chromatogr A 864:31-48. 1999
    ..The partitioning of three proteins, bovine serum albumin, lysozyme and apolipoprotein A-1, has been studied in the EO50PO50/HM-EOPO system and how the partitioning is affected by ..
  25. ncbi Towards a closed eye model of the pre-ocular tear layer
    R A Sack
    SUNY, College of Optometry, 100 East 20 Street, Manhattan, NY 10010, USA
    Prog Retin Eye Res 19:649-68. 2000
    ..shift from an inducible, neurologically controlled, lacrimal secretion containing among other proteins primarily lysozyme, lactoferrin and tear specific lipocalin, to a much slower, constitutive-type of secretion, composed almost ..
  26. ncbi Unusual chromatographic behaviour and one-step purification of a novel membrane proteinase from Bacillus cereus
    B Fricke
    Institute of Physiological Chemistry, Medical Faculty, Martin Luther University Halle-Wittenberg, Germany
    J Chromatogr A 715:247-58. 1995
    ..They remained localized in the cytoplasmic membrane after treatment with lysozyme and mutanolysin and they are strongly attached to the membrane compared with other known membrane proteinases...
  27. ncbi Aqueous two-phase systems containing self-associating block copolymers. Partitioning of hydrophilic and hydrophobic biomolecules
    M Svensson
    Department of Physical Chemistry 1, Lund University, Sweden
    J Chromatogr A 839:71-83. 1999
    ..The proteins were lysozyme, bovine serum albumin, cytochrome c, bacteriorhodopsin and the engineered B domain of staphylococcal protein A, ..
  28. ncbi True synovial metaplasia of breast implant capsules: a light and electron microscopic study
    A D del Rosario
    Department of Pathology and Laboratory Medicine, Samuel S Stratton VA Medical Center, Albany, New York, USA
    Ultrastruct Pathol 19:83-93. 1995
    ..Focal positive immunoreactivity was observed with alpha 1-antitrypsin, alpha 1-antichromotrypsin, lysozyme, and CD68...
  29. ncbi The influence of cell adsorbent interactions on protein adsorption in expanded beds
    H M Fernandez-Lahore
    Institut für Enzymtechnologie, Heinrich Heine Universitat Dusseldorf, Julich, Germany
    J Chromatogr A 873:195-208. 2000
    ..cerevisiae) on the adsorption of model proteins (hen egg white lysozyme and bovine serum albumin) to various stationary phases (cation and anion-exchange, hydrophobic interaction, ..
  30. ncbi Granulomatous slack skin in childhood
    F M Camacho
    Department of Medical-Surgical Dermatology, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
    Pediatr Dermatol 14:204-8. 1997
    ..The phenotype of the giant cells was lysozyme positive, CD68+ and Mac387-...
  31. pmc Antimicrobial activity of innate immune molecules against Streptococcus pneumoniae, Moraxella catarrhalis and nontypeable Haemophilus influenzae
    Haa Yung Lee
    The Gonda Department of Cell and Molecular Biology, House Ear Institute, Los Angeles, California 90057, USA
    BMC Infect Dis 4:12. 2004
    ..epithelia, including those of the middle ear and eustachian tube, secrete antimicrobial effectors including lysozyme, lactoferrin and beta defensins-1 and -2...
  32. ncbi Filamentous aggregates in a meningioma
    V Jay
    Department of Pathology, Hospital for Sick Children, University of Toronto, Canada
    Ultrastruct Pathol 20:577-83. 1996
    ..The inclusions were nonreactive for keratin, lysozyme, alpha-1-antitrypsin, and ubiquitin...
  33. ncbi Thermosensitive copolymers of N-vinylimidazole as displacers of proteins in immobilised metal affinity chromatography
    A E Ivanov
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden
    J Chromatogr A 907:115-30. 2001
    ..5% was most efficient as a reusable displacer for IMAC of ovalbumin, lysozyme and other proteins of egg white on Cu2+-IDA-Sepharose...
  34. ncbi Reevaluation of the effect of lysoyzme on Escherichia coli employing ultrarapid freezing followed by cryoelectronmicroscopy or freeze substitution
    P Wild
    Institute of Veterinary Anatomy, University of Zurich, Switzerland
    Microsc Res Tech 39:297-304. 1997
    b>Lysozyme is able to lyse Gram-positive bacteria acting as muramidase on the peptidoglycan polymer. Gram-negative bacteria in vitro are not lysed by lysozyme...
  35. ncbi Interaction between tryptophan residues and hydrophobically modified dextran. Effect on partitioning of peptides and proteins in aqueous two-phase systems
    M Lu
    Department of Biochemistry, Lund University, Sweden
    J Chromatogr A 766:99-108. 1997
    ..of tryptophan, tryptophan-tryptophan, (tryptophan)3, poly(lysine, tryptophan), beta-galactosidase and lysozyme in polymer aqueous two-phase systems...
  36. doi Characterization and expression analysis of a goose-type lysozyme from the rock bream Oplegnathus fasciatus, and antimicrobial activity of its recombinant protein
    Ilson Whang
    Department of Life Sciences, College of Natural Sciences, Jeju National University, Jeju Special Self Governing Province 690 756, Republic of Korea
    Fish Shellfish Immunol 30:532-42. 2011
    b>Lysozyme (muramidase) represents an important defense molecule of the fish innate immune system...
  37. ncbi Giant cell tumor of tendon sheath: immunohistochemical study of 20 cases
    A Cavaliere
    Institute of Pathological Anatomy and Histology, First Chair, Perugia University, Italy
    Tumori 83:841-6. 1997
    ..A variable but usually weak positivity for alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme was also observed...
  38. ncbi Comparative studies on the isothermal characteristics of proteins adsorbed under batch equilibrium conditions to ion-exchange, immobilised metal ion affinity and dye affinity matrices with different ionic strength and temperature conditions
    G M Finette
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
    J Chromatogr A 763:71-90. 1997
    ..the influence of a range of experimental parameters on the isothermal characteristics of hen egg white lysozyme (HEWL) and human serum albumin (HSA) adsorbed to several different adsorbents has been examined...
  39. ncbi Middle ear adenoma is an amphicrine tumor: why call it adenoma?
    S Ketabchi
    Dipartimento di Patologia Umana ed Oncologia, Universita degli Studi di Firenze, Italia
    Ultrastruct Pathol 25:73-8. 2001
    ..stained with anti-vimentin antibodies and were focally positive for chromogranin A, neuron-specific enolase, lysozyme, and cytokeratins AE1/AE3...
  40. ncbi Inhibition of in vitro lysozyme release from human granulocytes and monocytes by non-steroidal anti-inflammatory agents
    B Haneberg
    Medical Department B, University of Bergen, Norway
    Acta Pathol Microbiol Immunol Scand C 95:149-53. 1987
    A selective zymosan-induced release of lysozyme from freshly prepared human blood granulocytes and monocytes was inhibited by indomethacin, sulindac, piroxicam and ibuprofen...
  41. ncbi The catalytic domain of a bacterial lytic transglycosylase defines a novel class of lysozymes
    A M Thunnissen
    Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands
    Proteins 22:245-58. 1995
    ..The X-ray structure of SLT70 showed that one of its domains is structurally related to lysozyme, although there is no obvious similarity in amino acid sequence...
  42. ncbi Colorectal adenomas produce lysozyme
    C A Rubio
    Gastrointestinal and Liver Research Laboratory, Department of Pathology, Karolinska Institute and Hospital, 171 76 Stockholm, Sweden
    Anticancer Res 23:5165-71. 2003
    b>Lysozyme is an innate non-immunologic antibacterial enzyme produced by the Paneth cells of the upper intestinal tract. Lysozyme is not normally secreted in the lower intestinal tract...
  43. ncbi "Aberrant elastic" in elastofibroma: an immunohistochemical and ultrastructural study
    H J Kahn
    Department of Pathology, Women s College Hospital, Toronto, Ontario, Canada
    Ultrastruct Pathol 19:45-50. 1995
    ..fetal skin and three cases of elastofibroma by light microscopy and immunohistochemistry using an antibody to lysozyme, and one case of elastofibroma by electron microscopy (EM)...
  44. ncbi Metanephric adenoma of the kidney: an electron microscopic and immunohistochemical study with quantitative DNA measurement by image analysis
    A Nonomura
    Pathology Section, Kanazawa University Hospital, School of Medicine, Kanazawa University, Ishikawa, Japan
    Ultrastruct Pathol 19:481-8. 1995
    ..The tumor cells were also positive for cytokeratin, lysozyme, and Leu 7, with predominantly cell membrane staining...
  45. ncbi Ultrastructural and immunohistochemical contribution to the histogenesis of human cardiac myxoma
    E Govoni
    Institute of Clinical Electron Microscopy, University of Bologna, Italy
    Ultrastruct Pathol 12:221-33. 1988
    ..filaments (vimentin, desmin, actin, myosin) as well as myoglobin and factor VIII-related antigen, albumin, and lysozyme. Eighty percent of myxoma cells were simultaneously positive for vimentin, desmin, and actin, whereas 30% of them ..
  46. pmc The extracytoplasmic function sigma factor SigV plays a key role in the original model of lysozyme resistance and virulence of Enterococcus faecalis
    André Le Jeune
    Laboratoire de Microbiologie de l Environnement EA956 USC INRA 2017, Universite de Caen Basse Normandie, Caen, France
    PLoS ONE 5:e9658. 2010
    ..One of the most important compounds of the host innate defense response against invading microorganisms is lysozyme. It is found in a wide variety of body fluids, as well as in cells of the innate immune system...
  47. pmc Endothelin-1 inhibits pre-stimulated tracheal submucosal gland secretion and epithelial albumin transport
    E Yurdakos
    Department of Physiology, St George s Hospital Medical School, London
    Br J Pharmacol 104:1050-6. 1991
    ..d.), submucosal gland secretion (including lysozyme secretion from serous cells) and active epithelial albumin transport...
  48. ncbi Modeling the velocity field of the electroosmotic flow in charged capillaries and in capillary columns packed with charged particles: interstitial and intraparticle velocities in capillary electrochromatography systems
    A I Liapis
    Department of Chemical Engineering and Biochemical Processing Institute, University of Missouri-Rolla, 65409-1230, USA
    J Chromatogr A 877:181-215. 2000
    ..The intraparticle Peclet number, Pe(int rap), for lysozyme was found to be greater than unity and this intraparticle convective mass transfer mechanism could contribute ..
  49. doi Molecular characterisation of a goose-type lysozyme gene in Atlantic cod (Gadus morhua L.)
    Atle N Larsen
    Department of Marine Biotechnology, Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway
    Fish Shellfish Immunol 26:122-32. 2009
    ..An Atlantic cod goose-type (g-type) lysozyme EST was identified in a suppression subtractive hybridisation (SSH) cDNA library and the full-length cDNA (codg1) ..
  50. ncbi Comparison of diffusion and diffusion-convection matrices for use in ion-exchange separations of proteins
    D C Nash
    Department of Chemical Engineering, University of Cambridge, UK
    J Chromatogr A 807:185-207. 1998
    ..The equilibrium binding capacities for lysozyme and IgG on the diffusion-convection matrices were substantially lower than the equilibrium binding capacities on ..
  51. ncbi Thin-layer ion-exchange chromatography of proteins
    Q Luo
    Department of Materials Science and Engineering, University of Utah, Salt Lake City 84112-0560, USA
    J Chromatogr A 816:97-105. 1998
    ..The net charges were calculated for four model proteins, albumin, transferrin, lactoferrin and lysozyme, under different pH values...
  52. ncbi The effect of mucine, IgA, urea, and lysozyme on the corrosion behavior of various non-precious dental alloys and pure titanium in artificial saliva
    H Bilhan
    Faculty of Dentistry, Department of Removable Prosthodontics Istanbul University, 34390 Capa, Istanbul, Turkey
    J Biomater Appl 22:197-221. 2007
    ..The organic constituents are IgA, mucine, urea, and lysozyme which are encountered in the highest amounts in saliva and the dental materials are titanium (Ti), Co-Cr-Mo and ..
  53. ncbi Noncovalent adducts of poly(ethylene glycols) with proteins
    I N Topchieva
    Lomonosov Moscow State University, Department of Chemistry, Leninskii Gory, 119899, Moscow, Russia
    Bioconjug Chem 11:22-9. 2000
    ..preparation of noncovalent complexes between poly(ethylene glycol) (PEG) and proteins (alpha-chymotrypsin (ChT), lysozyme, bovine serum albumine) under high pressure has been developed...
  54. pmc Lysozyme M deficiency leads to an increased susceptibility to Streptococcus pneumoniae-induced otitis media
    Jun Shimada
    Gonda Department of Cell and Molecular Biology, House Ear Institute, Los Angeles, CA, USA
    BMC Infect Dis 8:134. 2008
    b>Lysozyme is an antimicrobial innate immune molecule degrading peptidoglycan of the bacterial cell wall. Lysozyme shows the ubiquitous expression in wide varieties of species and tissues including the tubotympanum of mammals...
  55. pmc Molecular basis of resistance to muramidase and cationic antimicrobial peptide activity of lysozyme in staphylococci
    Silvia Herbert
    Microbial Genetics Department, University of Tubingen, Tubingen, Germany
    PLoS Pathog 3:e102. 2007
    ..of peptidoglycan by O-acetylation renders pathogenic staphylococci resistant to the muramidase activity of lysozyme. Here, we show that a Staphylococcus aureus double mutant defective in O-acetyltransferase A (OatA), and the ..
  56. ncbi Toroidal coil counter-current chromatography study of the mass transfer rate of proteins in aqueous-aqueous polymer phase system
    Y Ito
    Laboratory of Biophysical Chemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Chromatogr A 802:277-83. 1998
    ..constructed to measure the mass transfer rates of five samples including potassium dichromate, methylene blue, lysozyme, ovalbumin and human serum albumin...
  57. ncbi Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) continuous-bed protein chromatography
    M Zhang
    Department of Biochemical Engineering, Tianjin University, PR China
    J Chromatogr A 912:31-8. 2001
    ..0 mg/g. Moreover, the separation of proteins, i.e. lysozyme, hemoglobin and bovine serum albumin, is little affected by mobile-phase velocity up to 902...
  58. ncbi Affinity chromatography of proteins on non-porous copolymerized particles of styrene, methyl methacrylate and glycidyl methacrylate
    C H Chen
    Department of Chemical Engineering, National Chung Cheng University, Chiayi, Taiwan
    J Chromatogr A 921:31-7. 2001
    ..0 cm x 0.46 cm I.D. column. This affinity column was effective for the separation of turkey egg white lysozyme from a protein mixture...
  59. pmc Comparison of the microbicidal and muramidase activities of mouse lysozyme M and P
    Philipp Markart
    Zentrum fur Innere Medizin, Medizinische Klinik II, Klinikstrasse 36, 35392 Giessen, Germany
    Biochem J 380:385-92. 2004
    b>Lysozyme is one of the most abundant antimicrobial proteins in the airspaces of the lung. Mice express two lysozyme genes, lysozyme M and P, but only the M enzyme is detected in abundance in lung tissues...
  60. ncbi Hydrophobic charge induction chromatography: salt independent protein adsorption and facile elution with aqueous buffers
    S C Burton
    Department of Chemistry, Massey University, Palmerston North, New Zealand
    J Chromatogr A 814:71-81. 1998
    ..At higher ligand densities, chymosin, chymotrypsinogen and lysozyme were adsorbed independently of ionic strength...
  61. ncbi Capillary electrochromatography of proteins and peptides with porous-layer open-tubular columns
    X Huang
    Department of Chemical Engineering, Yale University, New Haven, CT 06520-8286, USA
    J Chromatogr A 858:91-101. 1999
    ..A mixture of lysozyme, cytochrome c, ribonuclease A and alpha-chymotrypsinogen A was separated isocratically by counterdirectional CEC ..
  62. ncbi Cumulative site-directed charge-change replacements in bacteriophage T4 lysozyme suggest that long-range electrostatic interactions contribute little to protein stability
    S Dao pin
    Institute of Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene 97403
    J Mol Biol 221:873-87. 1991
    Bacteriophage T4 lysozyme is a basic molecule with an isoelectric point above 9.0, and an excess of nine positive charges at neutral pH...
  63. ncbi Lysozyme expression in the rat parotid gland: light and electron microscopic immunogold studies
    L Staneva-Dobrovski
    Department of Neuroanatomy, University of Dusseldorf, Germany
    Histochem Cell Biol 107:371-81. 1997
    b>Lysozyme (muramidase) is capable of direct bacteriolytic action by hydrolyzing glycosidic bonds in bacterial cell walls...
  64. ncbi Neurosarcoidosis without systemic sarcoidosis
    N Sommer
    Department of Neurology, , Federal Republic of Germany
    Eur Arch Psychiatry Clin Neurosci 240:334-8. 1991
    ..mild lymphocytic pleocytosis, intrathecal production of IgG without oligoclonal bands, and a raised level of lysozyme. MRI revealed multiple contrast-enhanced granulomas at the base of the brain with partial involvement of ..
  65. ncbi Superporous agarose beads as a hydrophobic interaction chromatography support
    P E Gustavsson
    Department of Pure and Applied Biochemistry, Lund University, Sweden
    J Chromatogr A 830:275-84. 1999
    ..then compared with the homogeneous beads in the separation of a mixture of three model proteins (ribonuclease A, lysozyme and bovine serum albumin) at various superficial flow velocities from 30 to 600 cm/h...
  66. ncbi Dissolution rate of hen egg-white lysozyme crystal under microgravity
    N Niimura
    Advanced Science Research Center, Japan Atomic Energy Research Institute, Ibaraki Ken, Japan
    Biol Sci Space 15:S176. 2001
    ..To this end, the dissolution rate of a single crystal of hen egg-white lysozyme has been measured both under microgravity and on earth in order to study the mechanism of protein crystallization...
  67. ncbi Lysozyme overexpression in fundic gland polyps
    Carlos A Rubio
    Gastrointestinal and Liver Pathology Research Laboratory, Department of Pathology, Karolinska Institute and University Hospital, 17176, Stockholm, Sweden
    Anticancer Res 30:1021-4. 2010
    ..We recently found that FGPs express lysozyme, one of the natural defence substances against infection...
  68. ncbi Influence of anti-inflammatory flavonoids on degranulation and arachidonic acid release in rat neutrophils
    M Tordera
    , Universidad de Valencia, Facultad de Farmacia, Spain
    Z Naturforsch C 49:235-40. 1994
    ..apigenin, fisetin, kaempferol, luteolin and quercetin were the most potent inhibitors of beta-glucuronidase and lysozyme release. The first compound was also able to inhibit basal release...
  69. ncbi Granular cell tumor: an immunohistochemical study
    A Cavaliere
    Institute of Pathological Anatomy and Histology, Perugia University, Italy
    Tumori 80:224-8. 1994
    ..KP1 and PG-M1, as well as occasional and focal positivity for alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme. CONCLUSIONS: The immunophenotypic profile constantly observed could be the expression, on one hand, of the ..
  70. ncbi Multiple marker studies on a malignant fibrous histiocytoma with primary cutaneous localization
    S Moretti
    Clinica Dermatologica II, , Italia
    Tumori 74:609-15. 1988
    ..Cytoplasmic proteins (acid phosphatase, non specific esterase, alpha-1 antitrypsin, and lysozyme) suggestive of a mononuclear phagocyte origin were demonstrated in varying amounts in neoplastic cells ..
  71. ncbi Chemorepellent signaling through the PACAP/lysozyme receptor is mediated through cAMP and PKC in Tetrahymena thermophila
    D L Hassenzahl
    Cedarville University, Department of Science and Mathematics, OH 45314, USA
    J Comp Physiol A 187:171-6. 2001
    Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena...
  72. ncbi Contribution of axial dispersion to band spreading in perfusion chromatography
    A Geng
    Department of Chemical and Environmental Engineering, National University of Singapore, Singapore
    J Chromatogr A 918:37-46. 2001
    ..Elution chromatography of proteins (bovine serum albumin and lysozyme) under non-retained conditions on two kinds of reversed-phase perfusive supports (POROS R1/H and POROS R2/H), ..
  73. doi Synergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces
    Y H Samaranayake
    Oral Bio Sciences, Faculty of Dentistry, University of Hong Kong, Hong Kong
    Arch Oral Biol 54:115-26. 2009
    ..The antimycotic protein lysozyme, in saliva is an important host defense mechanism although its activity against Candida biofilms on denture ..
  74. ncbi Adsorption behaviour and surfactant elution of cationic salivary proteins at solid/liquid interfaces, studied by in situ ellipsometry
    Ida E Svendsen
    Biomedical Laboratory Science, Faculty of Health and Society, Malmo University, SE 205 06 Malmo, Sweden
    Colloids Surf B Biointerfaces 53:157-66. 2006
    Adsorption of the cationic salivary proteins lactoferrin, lactoperoxidase, lysozyme and histatin 5 to pure (hydrophilic) and methylated (hydrophobized) silica surfaces was investigated by in situ ellipsometry...
  75. ncbi Counter-current chromatographic separation of glycoprotein components from Morchella esculenta (L.) with a polymer phase system by a cross-axis coil planet centrifuge
    Y Wei
    Beijing Research Center for Separation and Purification Technologies of Natural Products, Beijing Institute of New Technology Application, China
    J Chromatogr A 917:347-51. 2001
    ..The performance of the apparatus was optimized with four standard proteins including pepsin, lysozyme, ovalbumin and hemoglobin and a polymer phase system composed of 12...
  76. ncbi Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support
    B Xue
    Department of Biochemical Engineering, Tianjin University, China
    J Chromatogr A 921:109-19. 2001
    ..The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin ..
  77. ncbi Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis
    W Li
    State Key Laboratory of Biochemical Engineering, Chinese Academy of Sciences, Beijing
    J Chromatogr A 905:299-307. 2001
    ..PEGylated ribonuclease A and lysozyme were selected as examples...
  78. ncbi Application of a beta-cyclodextrin sulfate-immobilized precolumn to selective on-line enrichment and separation of heparin-binding proteins by column-switching high-performance liquid chromatography
    K Ishimura
    Department of Drug Formulations, Kaken Pharmaceutical Co. Ltd, Shizuoka, Japan
    J Chromatogr A 769:209-14. 1997
    ..The system was successfully used for the selective separation of lysozyme in egg white...
  79. ncbi Visualising intraparticle protein transport in porous adsorbents by confocal microscopy
    A Ljunglöf
    Amersham Pharmacia Biotech, Uppsala, Sweden
    J Chromatogr A 813:387-95. 1998
    ..The procedure was performed for two different proteins (lysozyme and human IgG) on two different media for protein adsorption (SP Sepharose Fast Flow and SP Sepharose XL; ..
  80. ncbi Preparation and use of ion-exchange chromatographic supports based on perfluoropolymers
    G E McCreath
    Department of Chemical Engineering, University of Cambridge, UK
    J Chromatogr A 773:73-83. 1997
    ..The equilibrium adsorption capacities of SP-PVA-FEP were 31.8 and 25.2 mg ml-1 for lysozyme and IgG respectively while for DEAE-PVA-FEP, the equilibrium adsorption capacities were 14.9 and 9...
  81. pmc PAF-induced muscarinic cholinoceptor hyperresponsiveness of ferret tracheal smooth muscle and gland secretion in vitro
    S E Webber
    Department of Physiology, St George s Hospital Medical School, London
    Br J Pharmacol 105:230-7. 1992
    ..activating factor (PAF) were examined on methacholine-induced smooth muscle contraction, mucus volume and lysozyme outputs, and albumin transport across the tracheal epithelium. 2. Methacholine (0...
  82. doi Studies of lysozyme binding to histamine as a ligand for hydrophobic charge induction chromatography
    Qing Hong Shi
    Dept of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P R China
    Biotechnol Prog 26:134-41. 2010
    ..b>Lysozyme adsorption onto Histamine-Sepharose (HA-S) was studied by adsorption equilibrium and calorimetry to uncover the ..
  83. ncbi Concerted evolution of ruminant stomach lysozymes. Characterization of lysozyme cDNA clones from sheep and deer
    D M Irwin
    Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720
    J Biol Chem 265:4944-52. 1990
    ..M., Sidow, A., White, R., and Wilson, A.C. (1989) in The Immune Response to Structurally Defined Proteins: The Lysozyme Model (Smith-Gill, S.J., and Sercarz, E.E., eds) pp...
  84. ncbi [Inhibitory effect of egg white lysozyme on ceftazidime-induced release of endotoxin from Pseudomonas aeruginosa]
    Ai Hua Liang
    Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China
    Yao Xue Xue Bao 38:801-4. 2003
    To investigate the inhibitory effect of egg white lysozyme (LZM) on ceftazidime (CFT)-induced release of endotoxin from Pseudomonas aeruginosa.
  85. doi Interaction of nano-TiO2 with lysozyme: insights into the enzyme toxicity of nanosized particles
    Zhen Xu
    State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China
    Environ Sci Pollut Res Int 17:798-806. 2010
    ..In the present study, nano-TiO(2) was selected as the representative of nanomaterials and lysozyme as a representative for enzymes...
  86. ncbi Interaction of lysozyme with synthetic anti-lysozyme D1.3 antibody fragments studied by affinity chromatography and surface plasmon resonance
    E Lasonder
    Laboratory of Medical Microbiology, University of Groningen, Netherlands
    J Chromatogr A 676:91-8. 1994
    Synthetic antibody fragments of monoclonal anti-lysozyme antibody D1.3 have been tested on binding with hen egg white lysozyme using immunoaffinity chromatography and surface plasmon resonance...
  87. ncbi Size exclusion behavior of hydroxypropylcellulose beads with temperature-dependent porosity
    B P Adrados
    Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden
    J Chromatogr A 930:73-8. 2001
    ..In a swollen state the size of pore is large enough to accommodate lysozyme (mol. mass 14400) and alpha-chymotrypsin (mol. mass 21600) but not bovine serum albumin (mol. mass 67000)...
  88. ncbi Structural details at active site of hen egg white lysozyme with di- and trivalent metal ions
    Shu Jie Li
    Department of Biophysics, College of Physics Science, Nankai University, Tianjin 300071, People s Republic of China
    Biopolymers 81:74-80. 2006
    Metal binding to lysozyme has received wide interest...
  89. pmc Effect of flash-heat treatment on antimicrobial activity of breastmilk
    Caroline J Chantry
    Department of Pediatrics, University of California Davis Medical Center, Sacramento, California 95817, USA
    Breastfeed Med 6:111-6. 2011
    ..The objectives were to measure flash-heat's effect on antimicrobial properties of lactoferrin, lysozyme, and whole milk and on the digestive resistance of lactoferrin and lysozyme.
  90. ncbi Modification of polystyrenic matrices for the purification of proteins. Effect of the adsorption of poly(vinyl alcohol) on the characteristics of poly(styrene-divinylbenzene) beads for use in affinity chromatography
    D C Nash
    Department of Chemical Engineering, University of Cambridge, UK
    J Chromatogr A 758:53-64. 1997
    ..A dye ligand (Procion Blue MX-R) has been covalently attached to PVA-PSDVB matrix and the lysozyme capacities of the PVA-PSDVB matrix have been determined...
  91. ncbi Estimating disease resistance in insects: phenoloxidase and lysozyme-like activity and disease resistance in the cricket Gryllus texensis
    Shelley A Adamo
    Department of Psychology, Dalhousie University, 1355 Oxford Street, Halifax, NS, Canada B3H 4J1
    J Insect Physiol 50:209-16. 2004
    ..by examining the relationship between two common estimates of insect immunocompetence, phenoloxidase and lysozyme-like enzyme activity, and resistance to three common insect bacterial pathogens: Serratia marcescens, Serratia ..
  92. ncbi In vitro deposition of lysozyme on etafilcon A and balafilcon A hydrogel contact lenses: effects on adhesion and survival of Pseudomonas aeruginosa and Staphylococcus aureus
    Shangtong Zhang
    Biology Department, Georgia State University, Kell Hall, 24 Peachtree Center Avenue, Atlanta, GA 30303, USA
    Cont Lens Anterior Eye 28:113-9. 2005
    To compare lysozyme adsorption and absorption and bacterial adhesion interactions on conventional (etafilcon A) and silicone (balafilcon A) hydrogel contact lenses.
  93. ncbi Direct purification of lysozyme using continuous counter-current expanded bed adsorption
    R O Owen
    Department of Chemical Engineering, University of Cambridge, UK
    J Chromatogr A 757:41-9. 1997
    ..Purification of lysozyme from egg-whites and enriched bovine milk could be carried out continuously. The former was extracted in 90...
  94. ncbi Modification of polystyrenic matrices for the purification of proteins. III. Effects of poly(vinyl alcohol) modification on the characteristics of protein adsorption on conventional and perfusion polystyrenic matrices
    D C Nash
    Department of Chemical Engineering, University of Cambridge, UK
    J Chromatogr A 776:65-73. 1997
    ..The adsorption capacities of lysozyme and HSA on these Procion Yellow HE-3G dyed PVA modified PS-DVB matrices were measured at various flow-rates and ..
  95. pmc Solubility of lysozyme in polyethylene glycol-electrolyte mixtures: the depletion interaction and ion-specific effects
    Matjaz Boncina
    Faculty of Chemistry and Chemical Technology, University of Ljubljana, SI 1000 Ljubljana, Slovenia
    Biophys J 95:1285-94. 2008
    The solubility of aqueous solutions of lysozyme in the presence of polyethylene glycol and various alkaline salts was studied experimentally...
  96. doi Lysozymes and lysozyme-like proteins from the fall armyworm, Spodoptera frugiperda
    Michael Chapelle
    UMR INRA UM2 1133, Laboratoire Ecologie Microbienne des insectes et Interactions hôtes Pathogènes, Université de Montpellier 2, Montpellier Cedex 05, France
    Mol Immunol 47:261-9. 2009
    b>Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls...
  97. ncbi Studies of the hydrodynamic volume changes that occur during refolding of lysozyme using size-exclusion chromatography
    B Batas
    School of Chemical Engineering, University of Bath, UK
    J Chromatogr A 766:109-19. 1997
    A size-exclusion chromatography-based refolding process (SEPROS) has successfully been used to renature lysozyme at high concentrations...
  98. ncbi Adhesion of tear proteins to contact lenses and vials
    E O Keith
    Department of Math, Science and Technology, Nova Southeastern University, 3301 College Avenue, Fort Lauderdale, FL 33314, USA
    Biotechnol Appl Biochem 34:5-12. 2001
    ..Using spectrophotometry at 280 nm, vials containing two proteins (lysozyme and milk lipocalin) at three different concentrations were examined for changes in their protein concentrations ..
  99. ncbi [Pulmonary blastoma. Immunohistochemical characterization of heteromorphic tumor components]
    M Brockmann
    ,
    Pneumologie 44:605-6. 1990
    ..It was not possible to label any tumour cells with the antibodies anti-beta-HCG, anti-NSE, anti-lysozyme or anti-CEA...
  100. ncbi Two-step chromatographic procedure for the purification of hen egg white ovomucin, lysozyme, ovotransferrin and ovalbumin and characterization of purified proteins
    A C Awade
    Laboratoire de Recherches et de Technologie Laitière, Institut National de la Recherche Agronomique, Rennes, France
    J Chromatogr A 677:279-88. 1994
    ..The procedure involves a first-step purification of ovomucin and lysozyme by gel permeation on a Superose 6 Prep Grade column...
  101. ncbi Ultrastructural features of adenoma malignum of the uterine cervix: demonstration of gastric phenotypes
    K Ishii
    Division of Clinical Pathology, Nagano Cancer Detection Center, Matsumoto, Japan
    Ultrastruct Pathol 23:375-81. 1999
    ..Post-embedding, double-immunogold staining for gastric gland mucous cell mucin (HIK1083-reactive mucin) and lysozyme revealed localization exclusively to the matrix and to the core of the mucin granules, respectively, both in ..

Research Grants63

  1. Recombinant Antibodies from the Sea Lamprey
    ZEEV contact PANCER; Fiscal Year: 2010
    ..We have isolated VLRs with low micromolar affinity to hen egg lysozyme (HEL) and low nanomolar affinity to R-phycoerythrin...
  2. MECHANISMS OF SALMONELLA INVASION AND TRANSMIGRATION
    James E Casanova; Fiscal Year: 2013
    ..Recent evidence indicates the existence of a novel population of lysozyme-expressing dendritic cells unique to the Peyer's patch, that are the first cells targeted by Salmonella upon ..
  3. Alkylammonium Formate Mobile Phase Modifiers for Protein Liquid Chromatography
    NEIL DANIELSON; Fiscal Year: 2009
    ..to study the tertiary and secondary structures of model proteins such as cytochrome c, bovine serum albumin, and lysozyme in these ionic liquids...
  4. MOLECULAR STUDIES OF SANFILIPPO SYNDROME TYPE B
    ELIZABETH NEUFELD; Fiscal Year: 2010
    ..data obtained from microarray analyses, we used a finding in the feasibility experiment (a 7-fold increase of lysozyme transcript) as an example of how we would proceed from microarray data to a hypothesis for the significance of ..
  5. Molecular Engineering of Humanized Anti-Staphlococcal Lytic Enzymes
    Karl E Griswold; Fiscal Year: 2012
    ..One example is the human enzyme lysozyme (hLYS), which kills Gram-positive pathogens in part by hydrolysis of cell wall peptidoglycan and subsequent ..
  6. Epithelial PLUNC as a determinant of Airway Mucosal Antimicrobial Activity
    YUANPU PETER DI; Fiscal Year: 2012
    ..of secretory ducts and submucosal glands, sites that express high levels of other host defense proteins such as lysozyme and lactoferrin...
  7. Crystallization and structure determination of the mu-opioid receptor
    Roger K Sunahara; Fiscal Year: 2012
    ..A major breakthrough in GPCR structural biology was the incorporation of a stable protein, T4 lysozyme (T4L), into a structurally unstable region of a GPCR, the 3rd cytoplasmic loop(1)...
  8. Vadim Cherezov; Fiscal Year: 2014
    ..we will follow the highly successful strategy of replacing the third intracellular loop of the receptor with T4 lysozyme combined with crystallization in LCP, which has recently yielded high resolution structures of human b2 ..
  9. Lysozymes and immunity in Anopheles gambiae
    Susan M Paskewitz; Fiscal Year: 2010
    ..Silencing of the lysozyme c-1 gene results in a dramatic reduction in the number of mosquitoes that become infected with Plasmodium...
  10. Dan Feng; Fiscal Year: 2014
    ..for family A GPCRs was developed in the laboratory of ConfometRx co-founder Brian Kobilka: generating GPCR-T4 Lysozyme (GPCR-T4L) fusion proteins...
  11. Recovery of Proteins from Formalin-fixed Tissues under High Hydrostatic Pressure
    Carol B Fowler; Fiscal Year: 2010
    ..recently demonstrated nearly quantitative reversal of formaldehyde-induced protein adducts and cross-links when lysozyme tissue surrogates, a model for FFPE tissues, were processed under high hydrostatic pressure (45,000 psi) at 65-80 ..
  12. Nicholas Stephanopoulos; Fiscal Year: 2014
    ..PAgels can efficiently entrap a number of proteins for several weeks, regardless of size and charge, including lysozyme (which has the same size and charge as most neurotrophins)...
  13. Innate Resistance to Mucosal HSV Infection in Humans
    Christine M Posavad; Fiscal Year: 2010
    ..in the mucosal samples with a focus on defensins, secretory leukocyte protease inhibitor (SLPI), lactoferrin and lysozyme, mediators linked to defense against HSV infection...
  14. Gregory A Weiss; Fiscal Year: 2015
    ..Strategic choice of proteins for library formation, such as the highly crystallizable protein lysozyme and the exceptionally soluble protein S-crystallin, for phage display will help insure the success of the project;..
  15. Testing and improving alchemical techniques for predicting protein-ligand binding
    DAVID LOWELL MOBLEY; Fiscal Year: 2012
    ..aims are to (1) improve the accuracy of binding free energy techniques in a polar model binding site in T4 lysozyme, using alchemical techniques with new algorithms to overcome sampling problems;[(2) predict binding affinities of ..
  16. Martin Muschol; Fiscal Year: 2016
    ..We will use two distinct, medically relevant and natively folded amyloid proteins: lysozyme and b-2 microglobulin...
  17. Devarajan Thirumalai; Fiscal Year: 2014
    ..the proposed research will offer insights into the molecular basis of elasticity of Green Fluorescent Protein and Lysozyme and the dependence of folding routes in RNA and proteins on the precise way force is applied...
  18. DESIGN AND TESTING OF DOCKING ALGORITHMS
    Brian K Shoichet; Fiscal Year: 2011
    ..ability to predict new ligands and geometries for two model systems: AmpC beta-lactamase and a cavity site in lysozyme. Both proteins are easy to work with and provide well defined sites for docking studies...
  19. Protein Structure and Dynamics from EPR Spectroscopy and MD Simulations
    Terry P Lybrand; Fiscal Year: 2012
    ..refine structural models;and 4) apply the tools and strategies developed in Aims 1-3 to SDSL data obtained for T4 lysozyme, a model protein of known structure, for CDB3, a more complex erythrocyte membrane protein that is to date only ..
  20. Kenneth A Kudsk; Fiscal Year: 2015
    ..bombesin (BBS), in PN-fed mice increases intra-luminal and intra-Paneth cell innate immune molecules including lysozyme and [unreadable] defensin levels compared with PN feeding alone...
  21. Pneumococcal capsule and host innate immunity
    Moon H Nahm; Fiscal Year: 2012
    ..We also found that 11A is more resistant to lysozyme than 11E is and that L-ficolin binds to 11A but not to 11E...
  22. Dual Function of VPO1 in Pathogen Recognition and Killing
    Guangjie Cheng; Fiscal Year: 2013
    ..patterns (PAMPs), triggering serial downstream signaling events;alternatively, a number of enzymes such as lysozyme, digestive enzymes and defensins indiscriminately kill bacteria when the enzyme encounters the microbes...
  23. Luc Teyton; Fiscal Year: 2015
    ..Already shown for exogenous antigens such as gliadin and lysozyme, we will evaluate this mode of recognition for self-antigens such as BDC2...
  24. NITRIC OXIDE SIGNALING MECHANISMS IN VASCULAR CELLS
    Michael Mendelsohn; Fiscal Year: 2009
    ..To explore this hypothesis, we now have created mice expressing a LZ mutant (LZM) PKGIalpha defective for protein-protein interactions, but otherwise identical to wild-type PKGIa...
  25. Tanner J Freeman; Fiscal Year: 2015
    ..Using tissues from our murine models, differentiation (intestinal alkaline phosphatase, muc2, chromogranin A, lysozyme) and stem cell marker expression (Lgr5, Lrig1, Ascl2, Olfm4, DcamKL1, Mshi1) will be determined by qPCR and ..
  26. Epitope shifting and Antibody Maturation during Rotavirus Infection
    Benjamin W Spiller; Fiscal Year: 2012
    ..both for protein antigens and haptens, as exemplified by structural and biochemical studies using hen egg white lysozyme and small haptens. In these cases, affinity maturation improves binding to identical epitopes...
  27. Abdu F Azad; Fiscal Year: 2014
    ..Using qRT-PCR, we will compare defensin, lysozyme and a kunitz-type protease inhibitor (DvKPI) expression levels in uninfected and R. peacockii or R...
  28. Hui Chen Hsu; Fiscal Year: 2015
    ..The Ag transport function of MZP B cells will be studied using the hen egg lysozyme (HEL) or HEL-ovalbumin (OVA) conjugate strategies in combination with BXD2 mice that express the MD4 B-cell ..
  29. Investigations of the role of house flies as vectors for bacterial pathogens
    Dana Nayduch; Fiscal Year: 2009
    ..defense mechanisms in the midgut including physical barriers (peritrophic matrix) and secreted humoral defenses (lysozyme and antimicrobial peptides;AMPs)...
  30. STEVEN M M SOUTHWICK; Fiscal Year: 2015
    ..administration of a steroid, dexamethasone (dexamethasone suppression test);(3) measurement of the inhibition of lysozyme synthesis in blood cells by dexamethasone, and index of glucocorticoid receptor sensitivity in blood cells;(4) ..
  31. ANTIGEN PRESENTATION--ROLE OF IA MOLECULES
    EMIL RAPHAEL UNANUE; Fiscal Year: 2012
    ..In the case of lysozyme (HEL), the explanation centers on the editing role of H-2DM...
  32. Cardiac Myocycte-Role of PKG I Alpha in Aging-Dependent Cardiac Remodeling
    ROBERT MORRIS BLANTON; Fiscal Year: 2013
    ..mutations in the PKGI[unreadable] leucine zipper interacting domain (the PKGI[unreadable] leucine zipper mutant, or LZM, mouse)...
  33. Biology and Mechanism of the Single Subunit RNA Polymerases
    RUI J SOUSA; Fiscal Year: 2012
    ..To address these gaps in understanding we will: (1) Define the role of T7RNAP and its regulator T7 lysozyme in recruitment and assembly of the T7 DNA packaging machinery and, using both ensemble and single molecule ..
  34. STUDIES OF ANTIGEN STIMULATION
    Emil Unanue; Fiscal Year: 2009
    ..We investigated how the model protein antigen hen egg-white lysozyme (HEL) was processed by antigen presenting cells (APC);and established the chemical basis for the processing, ..
  35. BIOMOLECULAR FOLDING/RECOGNITION PROBED BY KINETIC EPR
    CHARLES SCHOLES; Fiscal Year: 2009
    ..T4 Lysozyme. Using rapid-mix submillisecond flow EPR, we will study bi-labeled T4 lysozyme to find the time scale for protein ..
  36. Nanoliter Lab-on-a-chip for Protein Crystallization
    Vamsee Pamula; Fiscal Year: 2007
    ..b>lysozyme, glucose isomerase, and proteinase K...
  37. Kurt F Hauser; Fiscal Year: 2014
    ..will be identified using floxed MOPr mice bred to astroglial specific GFAP, myeloid (microglial) specific lysozyme 2, and GABAergic forebrain neuron expressing Cre recombinase mice...
  38. Bioactivity and Vitamins in Flash-heated Breastmilk
    Caroline Chantry; Fiscal Year: 2005
    ..HIV-infected mothers: 1) vitamin content and protein digestibility; 2) key bioactive proteins (immunoglobulin A, lysozyme and lactoferrin); 3) antibacterial activity...
  39. Protein Stabilization in Ionic Liquids
    Gloria Elliott; Fiscal Year: 2009
    ..Aim #2: To identify the most solubilizing ILs for cytochrome C, lysozyme, and bovine serum albumin from the subset of ILs prioritized in Aim #1...
  40. Mechanisms of Immunological Adaptation to a Harsh Chemical Environment
    Charles Rice; Fiscal Year: 2009
    ..On the other hand, lymphoid COX-2 and lysozyme were greatly elevated compared to the reference population, indicating a state of macrophage/phagocyte activation...
  41. Formula with antimicrobials to decrease infant mortality
    Koteswara Chintalacharuvu; Fiscal Year: 2006
    ..S-IgA, lactoferrin and lysozyme in breast milk are the major antimicrobial factors...
  42. Resistance of Bacillus anthracis to lysozyme
    Jonathan Dworkin; Fiscal Year: 2009
    b>Lysozyme plays a central role in innate immunity by inhibiting extracellular bacterial viability, particularly on mucosal surfaces such as those of the lung...
  43. HOST DEFENSE FACTORS IN ORAL ECOLOGY
    JOEL RUDNEY; Fiscal Year: 1991
    Saliva contains a number of proteins which display antinicrobial activity in vitro. These include lysozyme (Lz), lactoferrin (Lf), salivary peroxidase (Spx), secretory immunoglobulin A (sIgA), the mucin glycoproteins (MG1 and MG2), and ..
  44. 250/500 GHZ GYROTRON BASED DNP/EPR SPECTROMETER
    RICHARD TEMKIN; Fiscal Year: 2002
    ..gyrotron-based spectrometer, we have demonstrated DNP enhanced magic angle spectra (MAS) of the protein T4 lysozyme. At ~55k we have achieved enhancements of ~50 in 15/N spectra...
  45. 250/500 GHZ GYROTRON BASED DNP/EPR SPECTROMETER
    RICHARD TEMKIN; Fiscal Year: 2003
    ..gyrotron-based spectrometer, we have demonstrated DNP enhanced magic angle spectra (MAS) of the protein T4 lysozyme. At ~55k we have achieved enhancements of ~50 in 15/N spectra...
  46. CRYSTAL STRUCTURES OF ANTIBODIES AND IDIOTYPIC CASCADE
    BRADFORD BRADEN; Fiscal Year: 1999
    ..mechanism of antibody/antigen recognition by determining the X-ray crystal structures of two related anti-hen egg lysozyme (HEL) antibodies, D44.1 and F10.6.6, that exhibit remarkable differences in affinity for the antigen...
  47. B Cell Repertoire and B cell Neoplasms in Old Mice
    MARC WEKSLER; Fiscal Year: 2006
    ..of antigen-specific mlg or diffuse large cell lymphoma (DLCL) from DNP-human serum albumin (DNP-HSA)- or hen egg lysozyme (HEL)-immunized C57/BL/6 mice...
  48. REGULATION AS A BASIS FOR ORGANISMAL DIFFERENCES
    JACK KIRSCH; Fiscal Year: 1993
    ..Two model systems are proposed that meet these requirements, namely the lysozyme and ribosomal DNA systems...
  49. REGULATION AS A BASIS FOR ORGANISMAL DIFFERENCES
    Allan Wilson; Fiscal Year: 1991
    ..Two model systems are proposed that meet these requirements, namely the lysozyme and ribosomal DNA systems...
  50. HELPER T CELL EPITOPE IMMUNODOMINANCE
    Samuel Landry; Fiscal Year: 2000
    ..T-cell epitopes in T4 lysozyme have been predicted on the basis of local instability...
  51. TH1/TH2 IMMUNITY IN YOUNG ADULT AND AGED MICE
    Thomas Forsthuber; Fiscal Year: 2001
    ..Recently the investigator has shown that immunization with a prototypic antigen, hen egg white lysozyme (HEL) injected in complete Freunds adjuvant (CFA), induces an immune response that is essentially pure Th1, while ..
  52. Mechanisms of Immunomodulation by anti-RBC antibodies
    JAMES ZIMRING; Fiscal Year: 2009
    ..These models were generated using transgenic mice expressing either hen egg lysozyme (mHEL) or glycophorin A (hGPA) on RBCs...
  53. Lipids: Effectors in Innate Immunity
    Edith Porter; Fiscal Year: 2005
    ..body surfaces and to the overlaying fluid, which is rich in mucins and antimicrobial (poly) peptides, for example lysozyme. However, though all body fluids also contain lipids, including free fatty acids and phospholipids, only in the ..
  54. Effects of Tobacco Smoke on Airway Bactericidal Activity
    Yuan Pu Di; Fiscal Year: 2004
    ..human DD4 gene is specifically expressed in serous cells of submucosal glands where bactericidal proteins such as lysozyme and lactoferrin are secreted...
  55. MULTIHORMONAL REGULATION OF GENE EXPRESSION
    MICHEL SANDERS; Fiscal Year: 1992
    ..The hormonal regulation of another egg white gene lysozyme will be used to distinquish between gene-specific regulatory mechanisms and more generally applicable events...
  56. GENETICS, STRUCTURE, AND EXPRESSION OF IA ANTIGENS
    Patricia Jones; Fiscal Year: 1993
    ..T cells specific for a myelin basic protein peptide, azobenzenearsonate-tyro-sine, or peptides from hen egg lysozyme, and I-E-restricted T cells specific for myoglobin...
  57. CELL INTERACTIONS IN IMMUNE RESPONSES IN VITRO
    CARL PIERCE; Fiscal Year: 1992
    ..Fluorescein conjugates of fowl gammaglobulin, hen egg lysozyme (HEL) and pigeon cytochrome C (PCC) and GAT will be used...
  58. GALACTOSYLTRANSFERASES--STRUCTURE AND REGULATION
    Keith Brew; Fiscal Year: 2003
    ..The binding of alpha-lactalbumin, a mammary specific protein that is homologous with lysozyme, modulates the specificity of the enzyme to allow it to catalyze lactose biosynthesis (galactose transfer to ..
  59. Marginal zone B lymphocytes and blood borne pathogens
    Shiv Pillai; Fiscal Year: 2004
    ..reason why some antigen-C3d fusions have been successful is because the antigen was targeted to MZ B cells, using lysozyme specific B cells and lysozyme-C3d fusion proteins as a model system...
  60. LYSOZYME SECRETION AND PANETH CELL FUNCTION
    Stanley Erlandsen; Fiscal Year: 1980
    ..The secretion of lysozyme and the lysozyme content of Paneth cells in vivo and in vitro will be quantitated by radioimmunoassay-..
  61. LYSOZYME CRYSTAL GROWTH KINETICS
    JAMES BAIRD; Fiscal Year: 1999
    ..the atomic scale physicochemical parameters controlling the rate of growth of the model protein, hen egg-white lysozyme. This protein has been chosen because: (1) It is available commercially with adequate purity...
  62. MOLECULAR BASIS OF ANTIGENICITY
    DAVID BENJAMIN; Fiscal Year: 1991
    ..of these crystal structures involve complexes of monoclonal antibody Fab fragments and the antigen- Hen Egg-white Lysozyme (HEL)...
  63. MECHANISMS THAT REGULATE B CELL TOLERANCE
    Timothy Behrens; Fiscal Year: 2005
    ..Breeding of the en egg lysozyme (HEL) transgenic model for B cell anergy onto the CD5 null background resulted in a spontaneous loss of B cell ..