Ku70

Summary

Gene Symbol: Ku70
Description: X-ray repair complementing defective repair in Chinese hamster cells 6
Alias: CTC75, CTCBF, G22P1, KU70, ML8, TLAA, 5'-dRP lyase Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, lupus Ku autoantigen protein p70, thyroid autoantigen 70kD (Ku antigen), thyroid autoantigen 70kDa (Ku antigen), thyroid-lupus autoantigen p70
Species: human

Top Publications

  1. ncbi Histone deacetylase inhibitors sensitize prostate cancer cells to agents that produce DNA double-strand breaks by targeting Ku70 acetylation
    Chang Shi Chen
    Division of Medicinal Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 67:5318-27. 2007
  2. ncbi The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers
    Josefa Cabrero
    Departamento de Genetica, Universidad de Granada, 18071 Granada, Spain
    Chromosome Res 15:1093-100. 2007
  3. doi NF-kappaB p65 regulates nuclear translocation of Ku70 via degradation of heat shock cognate protein 70 in pancreatic acinar AR42J cells
    Joo Weon Lim
    Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, Seoul 120 752, Republic of Korea
    Int J Biochem Cell Biol 40:2065-77. 2008
  4. ncbi Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
    J R Walker
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 412:607-14. 2001
  5. ncbi Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase
    Haim Y Cohen
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 305:390-2. 2004
  6. pmc Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex
    Jingxin Sun
    Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:4936-45. 2012
  7. pmc Ku recruits the XRCC4-ligase IV complex to DNA ends
    S A Nick McElhinny
    Department of Biochemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
    Mol Cell Biol 20:2996-3003. 2000
  8. ncbi Human Ku70 interacts with heterochromatin protein 1alpha
    K Song
    Department of Biochemistry, University of Ulsan College of Medicine, Seoul 138 736, Korea
    J Biol Chem 276:8321-7. 2001
  9. ncbi Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signaling
    Masashi Idogawa
    Chemotherapy Division and ADP Ribosylation in Oncology Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Res 67:911-8. 2007
  10. ncbi Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility
    Yi Ping Fu
    Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan
    Cancer Res 63:2440-6. 2003

Detail Information

Publications201 found, 100 shown here

  1. ncbi Histone deacetylase inhibitors sensitize prostate cancer cells to agents that produce DNA double-strand breaks by targeting Ku70 acetylation
    Chang Shi Chen
    Division of Medicinal Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA
    Cancer Res 67:5318-27. 2007
    ..whereby histone deacetylase (HDAC) inhibitors sensitize prostate cancer cells to DNA-damaging agents by targeting Ku70 acetylation...
  2. ncbi The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers
    Josefa Cabrero
    Departamento de Genetica, Universidad de Granada, 18071 Granada, Spain
    Chromosome Res 15:1093-100. 2007
    ..Immunofluorescence patterns for Ku70, a DNA-repair protein participating in the non-homologous end-joining (NHEJ) pathway, showed that this protein is ..
  3. doi NF-kappaB p65 regulates nuclear translocation of Ku70 via degradation of heat shock cognate protein 70 in pancreatic acinar AR42J cells
    Joo Weon Lim
    Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, College of Medicine, Yonsei University, Seoul 120 752, Republic of Korea
    Int J Biochem Cell Biol 40:2065-77. 2008
    Ku proteins such as Ku70 and Ku80 play key roles in multiple nuclear processes. Nuclear translocation of Ku70 is independent of Ku80 translocation and mediated by nuclear localization signal (NLS) receptors including importin-alpha...
  4. ncbi Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair
    J R Walker
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 412:607-14. 2001
    The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway...
  5. ncbi Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase
    Haim Y Cohen
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 305:390-2. 2004
    ..SIRT1 deacetylates the DNA repair factor Ku70, causing it to sequester the proapoptotic factor Bax away from mitochondria, thereby inhibiting stress-induced ..
  6. pmc Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex
    Jingxin Sun
    Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA
    J Biol Chem 287:4936-45. 2012
    ..MR), we established an in vitro DNA end resection system using a forked model dsDNA substrate and purified human Ku70/80, Mre11, Mre11/Rad50, and exonuclease 1 (Exo1)...
  7. pmc Ku recruits the XRCC4-ligase IV complex to DNA ends
    S A Nick McElhinny
    Department of Biochemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina
    Mol Cell Biol 20:2996-3003. 2000
    ..We have therefore defined cell-free conditions that reflect the genetic requirement for ligase IV in cellular end joining and consequently can explain in molecular terms why this factor is required...
  8. ncbi Human Ku70 interacts with heterochromatin protein 1alpha
    K Song
    Department of Biochemistry, University of Ulsan College of Medicine, Seoul 138 736, Korea
    J Biol Chem 276:8321-7. 2001
    ..It consists of a heterodimer of 70- and 80-kDa subunits. Recently we have demonstrated that Ku70 interacted with TRF2, a mammalian telomere-binding protein...
  9. ncbi Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signaling
    Masashi Idogawa
    Chemotherapy Division and ADP Ribosylation in Oncology Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
    Cancer Res 67:911-8. 2007
    ..Using immunoprecipitation and mass spectrometry, we found that two Ku proteins, Ku70 and Ku80, were also associated with the complex...
  10. ncbi Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility
    Yi Ping Fu
    Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan
    Cancer Res 63:2440-6. 2003
    ..genetic variation in the genome, to examine these hypotheses, we have genotyped 30 SNPs in all five NHEJ genes (Ku70, Ku80, DNA-PKcs, Ligase IV, and XRCC4) in 254 primary breast cancer patients and 379 healthy controls...
  11. pmc SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70
    Nagalingam R Sundaresan
    University of Chicago Medical Center, Committee on Cellular and Molecular Physiology, Biological Science Division, University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 28:6384-401. 2008
    ..We also identified Ku70 as a new target of SIRT3...
  12. pmc The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation
    Anthony J Davis
    Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 288:7037-46. 2013
    ..DNA-PK is a holoenzyme consisting of a DNA-binding (Ku70/Ku80) and catalytic (DNA-PKcs) subunit...
  13. ncbi Direct binding of AP-1 (Fos/Jun) proteins to a SMAD binding element facilitates both gonadotropin-releasing hormone (GnRH)- and activin-mediated transcriptional activation of the mouse GnRH receptor gene
    Errol R Norwitz
    Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:37469-78. 2002
    ....
  14. ncbi Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiation
    Iraimoudi S Ayene
    Department of Radiation Oncology, School of Medicine, University of Pennsylvania, 195 John Morgan Building, Philadelphia, PA 19104 6072, USA
    Mol Cancer Ther 4:529-36. 2005
    Ku protein is a heterodimer (Ku70 and Ku86) known to play an important role in V(D)J recombination, apoptosis, telomere fusion, and double-strand break repair...
  15. ncbi DNA-PK phosphorylation sites on Oct-1 promote cell survival following DNA damage
    C Schild-Poulter
    Department of Medicine, The Ottawa Health Research Institute, University of Ottawa, Ottawa, Ontario, Canada
    Oncogene 26:3980-8. 2007
    ..These results implicate Oct-1 as a primary effector in a DNA-PK-dependent cell survival pathway that is activated by double-stranded DNA breaks...
  16. pmc Identification of an acetylation-dependant Ku70/FLIP complex that regulates FLIP expression and HDAC inhibitor-induced apoptosis
    E Kerr
    Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen s University Belfast, Northern Ireland, UK
    Cell Death Differ 19:1317-27. 2012
    ..We report a novel interaction between FLIP and the DNA repair protein Ku70 that regulates FLIP protein stability by inhibiting its polyubiquitination...
  17. ncbi Bax-inhibiting peptide protects cells from polyglutamine toxicity caused by Ku70 acetylation
    Y Li
    Department of Neurology and Neurological Science, Tokyo Medical and Dental University, Tokyo, Japan
    Cell Death Differ 14:2058-67. 2007
    ..Here, we show that expanded polyQ of ataxin-3 (Q79C), a gene that causes MJD, stimulates Ku70 acetylation, which in turn dissociates the proapoptotic protein Bax from Ku70, thereby promoting Bax activation and ..
  18. pmc Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1
    Navid Madani
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97201 3098, USA
    J Virol 76:11133-8. 2002
    ..Our results implicate Sp140 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif...
  19. pmc Poly(ADP-ribose) Polymerase 1 Interacts with Nuclear Respiratory Factor 1 (NRF-1) and Plays a Role in NRF-1 Transcriptional Regulation
    Mohammad B Hossain
    Department of Biochemistry and Molecular Biology, Genes and Development Program of the Graduate School of Biomedical Sciences, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 284:8621-32. 2009
    ..DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex...
  20. ncbi Actinomycin D induces histone gamma-H2AX foci and complex formation of gamma-H2AX with Ku70 and nuclear DNA helicase II
    Hannah Elisabeth Mischo
    Departments of Biochemistry and Molecular Biology, Institute of Molecular Biotechnology, Postfach 100 813, D 07708 Jena, Germany
    J Biol Chem 280:9586-94. 2005
    ..5 microg/ml DNA intercalating agent actinomycin D. In untreated cells, gamma-H2AX co-immunoprecipitated with Ku70, a subunit of DNA-dependent protein kinase, as well as with nuclear DNA helicase II (NDH II), a DEXH family ..
  21. doi Expression of TRF1, TRF2, TIN2, TERT, KU70, and BRCA1 proteins is associated with telomere shortening and may contribute to multistage carcinogenesis of gastric cancer
    Hua Hu
    Cancer Research Institute, The Second Affiliated Hospital, University of South China, 421001, Hengyang, Hunan, People s Republic of China
    J Cancer Res Clin Oncol 136:1407-14. 2010
    ..To elucidate the carcinogenesis mechanism in gastric cancer, the expression of telomeric proteins and changes in telomere length were investigated during multistage carcinogenesis of gastric cancer...
  22. pmc Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis
    Huafei Zou
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e39379. 2012
    ..In vitro studies demonstrate that caveolin-1 is a novel Ku70-binding protein, as shown by the binding of the scaffolding domain of caveolin-1 (amino acids 82-101) to the ..
  23. pmc An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins
    Maarten Hoek
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    J Biol Chem 286:10876-87. 2011
    ..of CAF-1-interacting proteins under mild conditions revealed that CAF-1 was directly associated with the KU70/80 complex, part of the DNA-dependent protein kinase, and the phosphoserine/threonine-binding protein 14-3-3 ΞΆ...
  24. ncbi Lupus autoantigen Ku protein binds HIV-1 TAR RNA in vitro
    W Kaczmarski
    Wistar Institute, Philadelphia, PA 19104
    Biochem Biophys Res Commun 196:935-42. 1993
    ....
  25. pmc Dynamic assembly of end-joining complexes requires interaction between Ku70/80 and XRCC4
    Pierre Olivier Mari
    Department of Biochemistry, Center for Biomics, Erasmus MC, University Medical Center, 3000 CA Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 103:18597-602. 2006
    ..Remarkably, the Ku heterodimers on DNA ends were in dynamic equilibrium with Ku70/80 in solution, showing that NHEJ complex assembly is reversible...
  26. ncbi Synthesis and functional analyses of nuclear clusterin, a cell death protein
    Konstantin S Leskov
    Department of Radiation Oncology, Laboratory of Molecular Stress Responses, Ireland Comprehensive Cancer Center and Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4942, USA
    J Biol Chem 278:11590-600. 2003
    Nuclear clusterin (nCLU) is an ionizing radiation (IR)-inducible protein that binds Ku70, and triggers apoptosis when overexpressed in MCF-7 cells. We demonstrate that endogenous nCLU synthesis is a product of alternative splicing...
  27. pmc HDAC6 deacetylates Ku70 and regulates Ku70-Bax binding in neuroblastoma
    Chitra Subramanian
    Department of Pediatrics, University of Michigan, Ann Arbor, MI 48109 0617, USA
    Neoplasia 13:726-34. 2011
    b>Ku70 was first characterized as a nuclear factor that binds DNA double-strand breaks in nonhomolog end-joining DNA repair...
  28. ncbi A topoisomerase IIbeta-mediated dsDNA break required for regulated transcription
    Bong Gun Ju
    Howard Hughes Medical Institute, Department of Medicine, School of Medicine, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093 0648, USA
    Science 312:1798-802. 2006
    ..Our data mechanistically link DNA topoisomerase IIbeta-dependent dsDNA breaks and the components of the DNA damage and repair machinery in regulated gene transcription...
  29. pmc The catalytic subunit of DNA-dependent protein kinase regulates proliferation, telomere length, and genomic stability in human somatic cells
    Brian L Ruis
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Cell Biol 28:6182-95. 2008
    ..protein kinase comprised of a 469-kDa catalytic subunit (DNA-PK(cs)) and the DNA binding regulatory heterodimeric (Ku70/Ku86) complex Ku...
  30. ncbi DNA-dependent protein kinase and XRCC4-DNA ligase IV mobilization in the cell in response to DNA double strand breaks
    Jerome Drouet
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077 Toulouse, Cedex 4, France
    J Biol Chem 280:7060-9. 2005
    ..DNA-PK comprises the Ku70/Ku80 heterodimer and the catalytic subunit DNA-PKcs...
  31. ncbi A role for DNA-PK in retroviral DNA integration
    R Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Science 284:644-7. 1999
    ..These results suggest that the initial events in retroviral integration are detected as DNA damage by the host cell and that completion of the integration process requires the DNA-PK-mediated repair pathway...
  32. pmc Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase
    Nicholas S Y Ting
    Department of Biochemistry and Molecular Biology, Cancer Biology Research Group, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Nucleic Acids Res 33:2090-8. 2005
    ..there is no sequence similarity between TLC1 and the RNA component (hTR) of human telomerase, we show that human Ku70/80 interacts with hTR both in vitro and in a cellular context...
  33. ncbi Ku80 binds to human replication origins prior to the assembly of the ORC complex
    Sahar Sibani
    McGill Cancer Center and Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Biochemistry 44:7885-96. 2005
    ..Interestingly, the association of Ku80 with these origins was not affected in this hypomorphic cell line, indicating that Ku and HsOrc-2 bind to origins independently of each other...
  34. doi FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16
    Kyu Heo
    Department of Biochemistry and Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089, USA
    Mol Cell 30:86-97. 2008
    ..Thus, these data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation...
  35. ncbi The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen
    T M Gottlieb
    Wellcome CRC Institute, Cambridge, England
    Cell 72:131-42. 1993
    ..The implications of the unusual DNA binding mode and multicomponent nature of the DNA-PK are discussed...
  36. pmc Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination
    Y Gu
    Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:8076-81. 1997
    ..The Ku70 protein is an additional component of DNA-PK; Ku70 forms a heterodimer with Ku80 to generate the DNA end-binding ..
  37. pmc Identification of poly(ADP-ribose)polymerase-1 and Ku70/Ku80 as transcriptional regulators of S100A9 gene expression
    Jens Grote
    Institute of Experimental Dermatology, University of Muenster, Germany
    BMC Mol Biol 7:48. 2006
    ..2002) J. Biol. Chem. 277, 41879-41887)...
  38. ncbi Ku, a DNA repair protein with multiple cellular functions?
    C Featherstone
    Wellcome Cancer Research Campaign Institute, Cambridge University, UK
    Mutat Res 434:3-15. 1999
    ..Ku also recruits DNA-PKcs to the DSB, activating its kinase function. Targeted disruption of the genes encoding Ku70 and Ku80 has identified significant differences between Ku-deficient mice and DNA-PKcs-deficient mice...
  39. pmc Functional significance of the interaction with Ku in DNA double-strand break recognition of XLF
    Ken ichi Yano
    Bioelectrics Research Center, Kumamoto University, 2 39 1 Kurokami, Kumamoto 860 8555, Japan
    FEBS Lett 585:841-6. 2011
    ..These results demonstrate the significance of Ku-XLF interaction in the molecular assembly of NHEJ factors...
  40. ncbi Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1
    Baomin Li
    Departments of Molecular Microbiology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 279:13659-67. 2004
    ..We have previously reported that WRN interacts with Ku70/80 and this interaction strongly stimulates WRN exonuclease activity...
  41. pmc The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit
    B K Singleton
    MRC Cell Mutation Unit, University of Sussex, Brighton BN1 9RR, United Kingdom
    Mol Cell Biol 19:3267-77. 1999
    ..This residue is conserved among species and lies outside of the previously reported Ku70-Ku80 interaction domain...
  42. ncbi Acetylation of the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis
    Haim Y Cohen
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115 USA
    Mol Cell 13:627-38. 2004
    ..The Ku70 DNA end-joining protein has recently been shown to suppress apoptosis by sequestering Bax from mitochondria...
  43. ncbi Three-dimensional structure of the human DNA-PKcs/Ku70/Ku80 complex assembled on DNA and its implications for DNA DSB repair
    Laura Spagnolo
    Section of Structural Biology and Cancer Research UK DNA Repair Enzyme Research Group, Institute of Cancer Research, Chester Beatty Laboratories, London
    Mol Cell 22:511-9. 2006
    ..DNA-PKcs is recruited to DSBs by the Ku70/Ku80 heterodimer, with which it forms the core of a multiprotein complex that promotes synapsis of the broken DNA ..
  44. ncbi In prostate cancer cells the interaction of C/EBPalpha with Ku70, Ku80, and poly(ADP-ribose) polymerase-1 increases sensitivity to DNA damage
    Hong Yin
    Feist Weiller Cancer Center and Department of Medicine, Health Sciences Center, Shreveport, Louisiana 71130 3932, USA
    J Biol Chem 281:11496-505. 2006
    ..b>Ku70, Ku80, and poly(ADP-ribose) polymerase-1 (PARP-1) were identified as proteins that associated with C/EBPalpha...
  45. pmc Regulation of the proapoptotic factor Bax by Ku70-dependent deubiquitylation
    Avigail D Amsel
    The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Proc Natl Acad Sci U S A 105:5117-22. 2008
    The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria...
  46. pmc Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus
    Parimal Karmakar
    Laboratory of Molecular Gerontology, Box 1, National Institute on Aging, IRP, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224 6825, USA
    Nucleic Acids Res 30:3583-91. 2002
    ..We have now mapped this interaction physically and functionally. The Ku70 subunit specifically interacts with the N-terminus (amino acids 1-368) of WRN, while the Ku80 subunit interacts ..
  47. pmc Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex
    Ioannis P Trougakos
    Laboratory of Molecular and Cellular Aging, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
    Clin Cancer Res 15:48-59. 2009
    ..However, the molecular mechanisms underlying its function remained largely unknown...
  48. ncbi Nuclear receptor coactivator thyroid hormone receptor-binding protein (TRBP) interacts with and stimulates its associated DNA-dependent protein kinase
    Lan Ko
    Department of Gene Regulation, Bone and Inflammation Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Biol Chem 278:11471-9. 2003
    ..revealed a DNA-dependent protein kinase (DNA-PK) complex including its catalytic subunit and regulatory subunits, Ku70 and Ku86...
  49. doi Oral cancer and genetic polymorphism of DNA double strand break gene Ku70 in Taiwan
    Da Tian Bau
    Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, 2 Yuh Der Road, Taichung 404, Taiwan
    Oral Oncol 44:1047-51. 2008
    The DNA repair gene Ku70, an important caretaker of the overall genome stability, is thought to play a major role in the DNA double strand break repair system...
  50. ncbi Selective inhibition of class switching to IgG and IgE by recruitment of the HoxC4 and Oct-1 homeodomain proteins and Ku70/Ku86 to newly identified ATTT cis-elements
    Andras Schaffer
    Division of Molecular Immunology, Department of Pathology and Laboratory Medicine, Joan and Sanford I Weill Medical College, Cornell University, New York, New York 10021, USA
    J Biol Chem 278:23141-50. 2003
    ..We show here that the HoxC4 and Oct-1 homeodomain proteins together with the Ku70/Ku86 heterodimer bind as a complex to newly identified switch (S) regulatory ATTT elements (SREs) in the Igamma and ..
  51. doi Ku antigen interacts with abasic sites
    Ekaterina S Ilina
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospect Lavrentieva 8, 630090, Novosibirsk, Russia
    Biochim Biophys Acta 1784:1777-85. 2008
    ..Ku antigen as a part of DNA-PK was shown to inhibit AP site cleavage by apurinic/apyrimidinic endonuclease 1...
  52. pmc DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner
    Eun Jung Park
    Division of Molecular Life Sciences and Center for Cell Signalling Research, Ewha Womans University, Seoul 120 750, Korea
    Nucleic Acids Res 31:6819-27. 2003
    ....
  53. pmc Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair
    John B Leppard
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:5919-27. 2003
    ....
  54. ncbi Functional interaction between Ku and the werner syndrome protein in DNA end processing
    B Li
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 275:28349-52. 2000
    ..Peptide sequence analysis indicates that the two proteins are identical to Ku70 and Ku80, a heterodimer involved in double strand DNA break repair by non-homologous DNA end joining...
  55. ncbi Human Ku70/80 associates physically with telomerase through interaction with hTERT
    Weihang Chai
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9039, USA
    J Biol Chem 277:47242-7. 2002
    ..The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance...
  56. ncbi Regulation of the pancreatic duodenal homeobox-1 protein by DNA-dependent protein kinase
    Patricia Lebrun
    INSERM U145, Faculty of Medicine, IFR50 Nice, France
    J Biol Chem 280:38203-10. 2005
    ..We identified new proteins associated with PDX-1 by mass spectrometry. These proteins, Ku70 and Ku80, are regulatory subunits of DNA-dependent protein kinase (DNA-PK)...
  57. ncbi Pterygium and genetic polymorphism of DNA double strand break repair gene Ku70
    Yi Yu Tsai
    Department of Ophthalmology, China Medical University, Taichung, Taiwan
    Mol Vis 13:1436-40. 2007
    ..In humans, NHEJ is the predominant repair system and Ku70 protein plays an initial and important role in the NHEJ system...
  58. ncbi Ku binds telomeric DNA in vitro
    A Bianchi
    Rockefeller University, New York, New York 10021, USA
    J Biol Chem 274:21223-7. 1999
    ..Our results indicate that the DNA binding characteristics of Ku are consistent with its direct interaction with telomeric DNA in mammalian cells and its proposed role as a telomere end factor...
  59. pmc Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair
    M N Paddock
    Seattle Children s Hospital Research Institute, 1900 9th Ave, Seattle, WA 98101, USA
    DNA Repair (Amst) 10:338-43. 2011
    ..Here we show that immunoprecipitation of the BRCT domain of PARP-1 pulls down Ku70 and the DNA-PK complex although the BRCT domain of PARP-1 does not bind DNA, suggesting that this interaction is ..
  60. ncbi Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells
    Greg L Mayeur
    Department of Biological Chemistry, School of Medicine, University of California, Davis, UC Davis Cancer Center, Sacramento, California 95817, USA
    J Biol Chem 280:10827-33. 2005
    ..AR directly interacts with both Ku70 and Ku80 in vivo and in vitro, as shown by co-immunoprecipitation, glutathione S-transferase pull-down, and Sf9 ..
  61. ncbi Interaction of human Ku70 with TRF2
    K Song
    Department of Biochemistry, University of Ulsan College of Medicine, Seoul, South Korea
    FEBS Lett 481:81-5. 2000
    ..We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80...
  62. ncbi Ku autoantigen (DNA helicase) is required for interleukins-13/-4-induction of 15-lipoxygenase-1 gene expression in human epithelial cells
    U P Kelavkar
    Center for Glomerulonephritis, Renal Division, Emory University, Atlanta, GA, USA
    Genes Immun 1:237-50. 2000
    ..proteins, identified one as the 86 kDA Lupus KU autoantigen protein P86 and the second as the 70 kDa Lupus KU autoantigen protein P70. Gel shift and supershift experiments using monoclonal antibodies toward Ku antigen and its ..
  63. ncbi Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences
    S Galande
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA
    J Biol Chem 274:20521-8. 1999
    ..These results provide a mechanistic link toward understanding the functional overlap of PARP and DNA-PK and suggest a novel role for these proteins in the regulation of chromatin structure and function...
  64. pmc Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines
    Gregory Nolens
    Laboratory of Molecular Oncology, GIGA Cancer, University of Liege, B34, Avenue de l Hopital, Liege, 4000, Belgium
    Breast Cancer Res 11:R83. 2009
    ..In order to understand the mechanism by which the ERBB2 gene is overexpressed we searched for novel AP-2 interacting factors that contribute to its activity...
  65. ncbi DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer
    D W Chan
    Department of Biological Sciences, University of Calgary, Canada
    Biochemistry 38:1819-28. 1999
    Ku antigen is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively) that together bind with high affinity to ends of double-stranded DNA and other DNA structures in vitro...
  66. pmc Ku regulates signaling to DNA damage response pathways through the Ku70 von Willebrand A domain
    Victoria L Fell
    Robarts Research Institute and Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada
    Mol Cell Biol 32:76-87. 2012
    The Ku heterodimer (Ku70/Ku80) is a main component of the nonhomologous end-joining (NHEJ) pathway that repairs DNA double-strand breaks (DSBs)...
  67. ncbi Expression of human glucocorticoid receptor gene and interaction of nuclear proteins with the transcriptional control element
    N Warriar
    Department of Molecular Endocrinology, Laval University Hospital Center, 2705 Boulevard Laurier, Sainte Foy, G1V 4G2, Quebec, Canada
    J Biol Chem 271:18662-71. 1996
    ..Both Ku80 and Ku70 polypeptides were present in high concentrations in human placenta and HeLa cells...
  68. pmc Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions
    S Jin
    Division of Tumor Immunology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    EMBO J 16:6874-85. 1997
    Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA-dependent protein kinase (DNA-PK)...
  69. pmc Depletion of Ku70/80 reduces the levels of extrachromosomal telomeric circles and inhibits proliferation of ALT cells
    Baomin Li
    Department of Molecular Microbiology and Immunology, Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Aging (Albany NY) 3:395-406. 2011
    ..Here we show that shRNA-mediated knockdown of the Ku70/80 heterodimer, a factor with functions at both pathological and natural DNA ends, inhibits ALT cell growth and ..
  70. pmc Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication
    Yingfeng Zheng
    Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R3E 0J9, Canada
    J Biol Chem 286:17722-35. 2011
    ..In the present study, we provide evidence that the host protein Ku70 interacts with HIV-1 IN and protects it from the Lys(48)-linked polyubiquitination proteasomal pathway...
  71. pmc Proteomics of herpes simplex virus replication compartments: association of cellular DNA replication, repair, recombination, and chromatin remodeling proteins with ICP8
    Travis J Taylor
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    J Virol 78:5856-66. 2004
    ..In Ku70-deficient murine embryonic fibroblasts, viral yields are increased by almost 50-fold, suggesting that the cellular ..
  72. pmc Interleukin-1 beta-converting enzyme-like protease cleaves DNA-dependent protein kinase in cytotoxic T cell killing
    Q Song
    Queensland Cancer Fund Research Unit, Queensland Institute of Medical Research, Bancroft Centre, PO Royal Brisbane Hospital, Herston, Australia
    J Exp Med 184:619-26. 1996
    ..This study demonstrates for the first time that the cleavage of DNA-PKcs in this intact cell system is exclusively due to an ICE-like protease...
  73. ncbi Positive and negative modulation of the transcriptional activity of the ETS factor ESE-1 through interaction with p300, CREB-binding protein, and Ku 70/86
    Hong Wang
    Cardiology Division, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 279:25241-50. 2004
    ..We have determined that Ku70, Ku86, p300, and CREB-binding protein (CBP) are ESE-1 interacting proteins...
  74. pmc The Ku protein complex interacts with YY1, is up-regulated in human heart failure, and represses alpha myosin heavy-chain gene expression
    Carmen C Sucharov
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
    Mol Cell Biol 24:8705-15. 2004
    ..Through peptide mass spectrometry, we identified this binding activity to be a heterodimer of Ku70 and Ku80. Expression of Ku represses the human alphaMyHC promoter in neonatal rat ventricular myocytes...
  75. pmc Expression of apolipoprotein C-IV is regulated by Ku antigen/peroxisome proliferator-activated receptor gamma complex and correlates with liver steatosis
    Eun Kim
    Liver Research Center, Rhode Island Hospital and Warren Alpert Medical School of Brown University, Providence, RI 02903, USA
    J Hepatol 49:787-98. 2008
    ..7-fold increase), a member of the apolipoprotein family implicated in liver steatosis. Here, we identified host transcription factors regulating the ApoC-IV gene expression...
  76. ncbi The three-dimensional structure of the C-terminal DNA-binding domain of human Ku70
    Z Zhang
    Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    J Biol Chem 276:38231-6. 2001
    The proteins Ku70 (69.8 kDa) and Ku80 (82.7 kDa) form a heterodimeric complex that is an essential component of the nonhomologous end joining DNA double-strand break repair pathway in mammalian cells...
  77. pmc Synapsis of DNA ends by DNA-dependent protein kinase
    Lisa G DeFazio
    Departments of Medicine and Biochemistry, Stanford University, Stanford, CA 94305, USA
    EMBO J 21:3192-200. 2002
    ..Our results suggest that DNA-PK(CS) brings DNA ends together and then undergoes activation of its kinase, presumably to regulate subsequent steps for processing and ligation of the ends...
  78. pmc TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1
    Caroline Canfield
    Department of Biochemistry and Molecular Biology and the Feist Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, 71130, USA
    BMC Mol Biol 10:110. 2009
    ..However, the phosphorylation of Rad9(S328) by TLK1B appeared important for mediating a cell cycle checkpoint, and thus, this phosphorylation of Rad9 may have other effects on 9-1-1 functionality...
  79. doi Micronucleus formation, DNA damage and repair in premenopausal women chronically exposed to high level of indoor air pollution from biomass fuel use in rural India
    Nandan Kumar Mondal
    Department of Experimental Hematology, Chittaranjan National Cancer Institute, 37, S P Mukherjee Road, Kolkata 700026, West Bengal, India
    Mutat Res 697:47-54. 2010
    ..damage was examined by comet assay in peripheral blood lymphocytes (PBL); and expressions of gamma-H2AX, Mre11 and Ku70 proteins were localized in AEC and PBL by immunocytochemistry...
  80. doi NBS1 regulates a novel apoptotic pathway through Bax activation
    Kenta Iijima
    Department of Environmental Sciences, Faculty of Science, Ibaraki University, Bunkyo 2 1 1, Mito, Ibaraki, Japan
    DNA Repair (Amst) 7:1705-16. 2008
    ..Interestingly, NBS1 regulates a novel Bax activation pathway by disrupting the Ku70-Bax complex which is required for activation of the mitochondrial apoptotic pathway...
  81. ncbi Involvement of the nonhomologous end joining DNA repair pathway in the bystander effect for chromosomal aberrations
    John B Little
    Laboratory of Radiobiology, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115, USA
    Radiat Res 159:262-7. 2003
    Cells of mouse knockout cell lines for Ku80 (now known as Xrcc5), Ku70 (now known as G22p1), DNA-PKcs (now known as Prkdc) and PARP (now known as Adprt) were synchronized in G1 phase and exposed to very low fluences of alpha particles...
  82. pmc The radiosensitizing effect of Ku70/80 knockdown in MCF10A cells irradiated with X-rays and p(66)+Be(40) neutrons
    Veerle Vandersickel
    Department of Structural and Functional Biology, Laboratory of Toxicology and Pharmacology, Universita degli Studi dell Insubria, Via A, Da Guissano 10, 21052 Busto Arsizio, Italy
    Radiat Oncol 5:30. 2010
    ..To date however, our knowledge regarding the importance of DNA DSB repair proteins and mechanisms in the response of human cells to high-LET radiation, is far from being complete...
  83. pmc Ku70 is required for late B cell development and immunoglobulin heavy chain class switching
    J P Manis
    Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    J Exp Med 187:2081-9. 1998
    ..b>Ku70 and Ku80 form a DNA end-binding complex required for DNA double strand break repair and V(D)J recombination...
  84. ncbi Reduced DNA double strand breaks in chlorambucil resistant cells are related to high DNA-PKcs activity and low oxidative stress
    Istvan Boldogh
    Department of Microbiology and Immunology, Sealy Center for Molecular Sciences, University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA
    Toxicology 193:137-52. 2003
    ..The decreased kinase activity of DNA-PKcs was not due to a change in its amount or the levels of Ku70 and Ku86, their subcellular distribution, cell cycle progression or caspase-mediated degradation of DNA-PK...
  85. doi Ku, Artemis, and ataxia-telangiectasia-mutated: signalling networks in DNA damage
    Tomohiro Morio
    Department of Pediatrics and Developmental Biology, Tokyo Medical and Dental University, Graduate School of Medicine, Tokyo 113 8519, Japan
    Int J Biochem Cell Biol 40:598-603. 2008
    ..Once the DNA is damaged, the DNA repair protein Ku70/80 translocates into the nucleus, a process which may be mediated by ataxia-telangiectasia-mutated, a member of the ..
  86. ncbi Bcr-Abl-independent imatinib-resistant K562 cells show aberrant protein acetylation and increased sensitivity to histone deacetylase inhibitors
    Sang Min Lee
    Department of Biochemistry, Research Center for Ischemic Tissue Regeneration, College of Medicine, Pusan National University, Pusan 602 739, South Korea
    J Pharmacol Exp Ther 322:1084-92. 2007
    ..resistance mechanism in these variants also included aberrant acetylation of nonhistone proteins such as p53, Ku70, and Hsp90 that was due to upregulation of histone deacetylases (HDACs) and down-regulation of histone ..
  87. ncbi The DNA-dependent protein kinase interacts with DNA to form a protein-DNA complex that is disrupted by phosphorylation
    Dennis Merkle
    Department of Biochemistry and Molecular Biology, University of Calgary, 3300 Hospital Drive, Calgary AB, T2N 4N1, Canada
    Biochemistry 41:12706-14. 2002
    ..DNA-PK is composed of a large catalytic subunit, DNA-PKcs, and a heterodimer of Ku70 and Ku80 subunits...
  88. pmc Regulation of telomere length by fatty acid elongase 3 in yeast. Involvement of inositol phosphate metabolism and Ku70/80 function
    Suriyan Ponnusamy
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 283:27514-24. 2008
    ....
  89. doi Mitomycin C modulates DNA-double strand break repair genes in cervical carcinoma cells
    Yun Hee Kang
    Laboratory of Cell and Immuno biochemistry, Department of Bioscience and Biotechnology, Bio Molecular Informatics Center, Konkuk University, Hwayang Dong 1, Gwangjin gu, Seoul, 143 701, South Korea
    Amino Acids 39:1291-8. 2010
    ..Three spots have not been identified in the database. Ku70-binding protein (KUB3), MHC class I antigen, MHC class I chain-related protein A or multi-PDZ domain protein 1, ..
  90. ncbi Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae
    Tadashi Takahashi
    Noda Institute for Scientific Research, 399 Noda, 278 0037, Noda City, Chiba, Japan
    Mol Genet Genomics 275:460-70. 2006
    ..that DNA integration occurs predominantly through a nonhomologous end joining pathway where two ku genes, namely, ku70 and ku80, play a key role...
  91. pmc The Drosophila melanogaster DmRAD54 gene plays a crucial role in double-strand break repair after P-element excision and acts synergistically with Ku70 in the repair of X-ray damage
    R Kooistra
    Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Biol 19:6269-75. 1999
    ..P-element excision involves the formation of two DSBs which have identical 3' overhangs of 17 nucleotides. The crucial role of homologous recombination in the repair of these DSBs may be related to the very specific nature of the breaks...
  92. ncbi Modulation of Ku70/80, clusterin/ApoJ isoforms and Bax expression in indocyanine-green-mediated photo-oxidative cell damage
    Federico Ricci
    Section of Ophthalmology, University of Rome Tor Vergata, Rome, Italy
    Ophthalmic Res 39:164-73. 2007
    ..Cell viability and death induction were examined, as well as the modulation of proteins involved in cell death and DNA repair...
  93. pmc Alternative pathways for the repair of RAG-induced DNA breaks
    David M Weinstock
    Department of Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell Biol 26:131-9. 2006
    ..As expected, V(D)J recombination was substantially impaired in cells deficient for the NHEJ components Ku70, XRCC4, and DNA-PKcs...
  94. pmc Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70
    Cristian Boboila
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:3034-9. 2010
    ..In normal cells, many CSR junctions are mediated by classical nonhomologous end-joining (C-NHEJ), which employs the Ku70/80 complex for DSB recognition and XRCC4/DNA ligase 4 for ligation...
  95. ncbi 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis
    Kuniyoshi Iwabuchi
    Department of Biochemistry, Kanazawa Medical University, Daigaku 1 1, Uchinada, Kahoku gun, Ishikawa 920 0293, Japan
    Genes Cells 11:935-48. 2006
    ..These results demonstrate that the 53BP1-dependent repair pathway is important for survival of cells irradiated with IR during the G1 phase of the cell cycle...
  96. ncbi Differential regulation of Ku gene expression in etiolated mung bean hypocotyls by auxins
    Pei Feng Liu
    Department of Life Sciences and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsin Chu, Taiwan 30043, Republic of China
    Biochim Biophys Acta 1769:443-54. 2007
    ..In this study, the cDNAs encoding Ku70 (VrKu70) and Ku80 (VrKu80) were isolated from mung bean (Vigna radiata L.) hypocotyls...
  97. doi A role for endogenous and radiation-induced DNA double-strand breaks in p53-dependent apoptosis during cortical neurogenesis
    Hongzhe Li
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Radiat Res 169:513-22. 2008
    ..the neural developmental effects of acute radiation exposure in mouse littermate embryos harboring mutations in the Ku70 and p53 genes...
  98. doi A highly efficient gene-targeting system for Aspergillus parasiticus
    P K Chang
    Southern Regional Research Center, Agricultural Research Service, U S Department of Agriculture, New Orleans, LA 70124, USA
    Lett Appl Microbiol 46:587-92. 2008
    ..To establish a system that greatly increases the gene-targeting frequency in Aspergillus parasiticus...
  99. pmc Ku80 participates in the targeting of retroviral transgenes to the chromatin of CHO cells
    Christel Masson
    LBPA, CNRS, E N S Cachan, 61 Avenue du President Wilson, 94235 Cachan, France
    J Virol 81:7924-32. 2007
    The heterodimer Ku70/80 Ku is the DNA-binding component of the DNA-PK complex required for the nonhomologous end-joining pathway...
  100. pmc Interaction of a cyclin E fragment with Ku70 regulates Bax-mediated apoptosis
    Suparna Mazumder
    Department of Cancer Biology, Cleveland Clinic, Cleveland, OH 44195, USA
    Mol Cell Biol 27:3511-20. 2007
    ..Here we identify Ku70 as a specific p18-cyclin E-interacting partner...
  101. ncbi The non-homologous end-joining pathway is not involved in the radiosensitization of mammalian cells by heat shock
    Joseph R Dynlacht
    Department of Radiation Oncology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    J Cell Physiol 196:557-64. 2003
    ..Several reports indicate that the Ku70 and Ku80 subunits of the mammalian DNA-dependent protein kinase (DNA-PK), a complex involved in NHEJ, appear to be ..

Research Grants63

  1. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
    ..e., for SSBR, PARP, XRCC1 and polynucleotide kinase;for DSBR, RAD family proteins, XRCC2 and 3, BRCA1 and 2, Ku70 and 86, and DNAPK)...
  2. Cdk-2 Independent role of cyclin E in Cell survival
    Alexandru Almasan; Fiscal Year: 2013
    ..Our hypothesis, supported by preliminary data, is that CycE function is mediated by its interaction with Ku70, which we have identified as a novel p18CycE-binding partner...
  3. CryoEM Structural Studies of DNA-PKcs and Nonhomologous End Joining Complexes
    Phoebe L Stewart; Fiscal Year: 2013
    ..The Ku70/80 heterodimer is the first protein to recognize and bind DNA ends at double strand breaks and recruits DNA-PKcs to ..
  4. Mechanisms of acquired temozolomide resistance in glioblastoma multiforme
    Jann N Sarkaria; Fiscal Year: 2013
    ..Whole exome-seq and RNA-seq in TMZ-resistant models without MGMT elevation identified mutations in DNA ligase IV, Ku70 and Ku80, which are key components in the non-homologous end-joining (NHEJ) DNA repair pathway...
  5. Ku70 Binding Protein-5 (KUB5), a Novel Factor in Nonhomologous End Joining
    David A Boothman; Fiscal Year: 2013
    ..Using yeast two hybrid analyses, we identified Ku70 binding protein #5 [(Kub5)/Hera], a highly conserved human homolog of yeast rtt103 that terminates RNA ..
  6. Pathway Choice of DNA Double-Strand Break Repair
    David J Chen; Fiscal Year: 2013
    ..The NHEJ pathway utilizes several enzymes, including Ku70/80 and DNA-PKcs, that capture both DNA ends, bring them together in a synaptic complex, and facilitate direct ..
  7. Characterization of V(D)J cleavage and repair complexes
    PATRICK SWANSON; Fiscal Year: 2009
    ..biochemical analysis, and used these RAG preparations to obtain evidence that full-length RAG1 associates with the Ku70/Ku80 complex involved in NHEJ, possibly through novel interaction partners...
  8. Bax Activation Mechanism in the Cytosol
    Shigemi Matsuyama; Fiscal Year: 2012
    ..However, the molecular mechanism controlling Bax activation is not yet understood. We found that Ku70, a 70 kDa protein, binds and inhibits Bax in the cytosol of non-apoptotic cells...
  9. Role of the Werner syndrome protein complex in the metabolism of chromosome ends
    Lucio Comai; Fiscal Year: 2013
    ..Our biochemical studies have shown that WRN operates as a functional unit with the Ku70/80 heterodimer and have further demonstrated that loss of WRN function causes the production of telomeric circles ..
  10. PATHOGENESIS OF HEPATOCELLULAR CARCINOMA
    Nelson Fausto; Fiscal Year: 2010
    ..We have shown that the lack of the Ku70 component of NHEJ repair pathway accelerates HCC development in mice injected with diethylnitrosamine (DEN), and ..
  11. DNA-PKcs Phosphorylation on Hematopoietic Stem Cells Genome Maintenance
    Benjamin Ping Chi Chen; Fiscal Year: 2013
    ..by applicant): The DNA dependent protein kinase catalytic subunit (DNA-PKcs) and its DNA-binding partner the Ku70/80 heterodimer are the key components of the non-homologous end-joining (NHEJ) pathway...
  12. The Roles of Conserved outer-membrane proteins in SFG rickettsia pathogenesis
    Juan J Martinez; Fiscal Year: 2013
    ..We have identified the first rickettsial protein-host cell receptor pair (rOmpB-Ku70) that plays an important role in the establishment of a successful infection within target human cells...
  13. Role of VprBP in B cell development and V(D)J recombination
    Patrick C Swanson; Fiscal Year: 2013
    ..We have identified two NHEJ repair factors, Ku70 and Ku80, and components of an E3 ubiquitin ligase complex as interaction partners with full-length RAG1...
  14. Defining the role of a novel E3 ubiquitin ligase in V(D)J recombination
    Patrick C Swanson; Fiscal Year: 2011
    ..We have identified two NHEJ repair factors, Ku70 and Ku80, and components of an E3 ubiquitin ligase complex as interaction partners with full-length RAG1...
  15. Ku regulates non-homologous end joining pathways in human somatic cells
    Eric A Hendrickson; Fiscal Year: 2013
    ..We will focus heavily, but not exclusively, on the heterodimeric (Ku70 and Ku86 subunits) Ku DNA end-binding complex and LIGIII (DNA ligase III)...
  16. Mechansim of activation of innate immunity by ISS-DNA
    Wen Ming Chu; Fiscal Year: 2013
    ..In addition, recent discoveries demonstrate that Ku70 is a mammalian receptor for Rickettsia conorii, and HMGB1 acts as a crucial cytokine that mediates the response to ..
  17. ATP-Ubiquitin Dependent Proteolysis
    Arthur L Haas; Fiscal Year: 2011
    ..model for polyubiquitin chain formation by Mdm2, and examine the in vitro conjugating activity of MdmX with Ku70 and Ku80, bona fide substrates of this ligase...
  18. Non-Homologous End Joining Repair in Humans
    Suk Hee Lee; Fiscal Year: 2013
    ..Finally, we will examine whether Metnase directly interacts with the Ku70/80 complex...
  19. KU, TELOMERE MAINTENANCE AND CELLULAR SENESCENCE
    David Chen; Fiscal Year: 2004
    The goal of this proposal is to study the Ku heterodimer's (Ku70/80) role in telomere maintenance and cellular senescence in mammals...
  20. IDENTITY AND FUNCTION OF VERTEBRATE TELOMERE PROTEINS
    Carolyn Price; Fiscal Year: 2002
    ..We will investigate whether Ku70 and Rad54 are required for telomere maintenance and whether these proteins are telomere components...
  21. Pathogenesis of Muscular Dystrophies
    Jeffrey Boone Miller; Fiscal Year: 2013
    ..that aberrant activation of cell death - mediated by the pro-death protein Bax and its cytosolic binding partner Ku70 - appears to be a pathogenetic mechanism in at least three human muscular dystrophies: Congenital Muscular ..
  22. DNA STRAND BREAKS AND THE GENETIC BASIS OF LYMPHOMAS
    Gilbert Chu; Fiscal Year: 2001
    ..DSB repair requires four genes: XRCC4, XRCC5 (Ku86), XRCC6 (Ku70), and XRCC7 (DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase), the latter of which is mutated in ..
  23. ROLE OF DNA DEPENDENT PROTEIN KINASE IN DNA REPAIR
    Paul Labhart; Fiscal Year: 2002
    ..encoding the three components of the DNA-dependent protein kinase (DNA-PKcs), the 470-kD catalytic subunit and the Ku70/80 heterodimer...
  24. Humoral immunodeficiency in cancer-prone SENCAR mice
    Andrea Bottaro; Fiscal Year: 2001
    ..cycle control, such as the cell cycle checkpoint regulator, atm (which is mutated in ataxia telangiectasia) and the Ku70 DNA repair factor...
  25. TOPOISOMERASE DRUG ACTIONS AT NUCLEAR MATRIX DNA DOMAIN
    Daniel Fernandes; Fiscal Year: 2003
    ..Additional experiments are designed to quantitate the degrees of drug-induced RPA32 hyperphosphorylation and Ku70-DNA-Pkcs binding to replicating and nonreplicating DNA...
  26. Capillary DNA Sequencer/Genetic Analyzer
    LESTER CASHDOLLAR; Fiscal Year: 2003
    ..and Characterization of Human Homologs of Murine Histocompatibility Antigens, H60, H4, and H71; New Function of KU70 in Apoptosis Regulation; Role of PLCy1 in B Cell Development and Function...
  27. HOXBOXES, HOMEODOMAINS, AND OSTEOBLAST GENE EXPRESSION
    Dwight Towler; Fiscal Year: 2007
    ..osteopontin). Specific aims are: Aim1: To identify the molecular interactions between Msx2, Runx2, and Ku70 that control calvarial osteoblast gene expression, using the OC promoter as a model for study...
  28. In Vivo Image Guidance for Gene-Radiation Therapy
    Gloria Li; Fiscal Year: 2007
    ..The tumor's response to radiation will then be evaluated. In pilot studies, we have shown that antisense Ku70 or a dominant negative Ku70 fragment significantly radiosensitizes hypoxic tumor cells in vitro and in vivo, and ..
  29. Redox Control of Radiation Response
    John Biaglow; Fiscal Year: 2006
    ..protein thiols, including; pp59fyn, a cytoplasmic signaling protein; caspase-3, a pro-apoptotic caspase; and Ku70, a component of the DNA damage recognition and repair machinery...
  30. EPITHELIAL CELLS IN LUNG INFLAMMATION
    Peter Kao; Fiscal Year: 2003
    ..The CsA-sensitive purine-box regulator in HBE and T-cells contains subunits, NF45, NF90, Ku70, Ku80, and the DNA-dependent protein kinase, catalytic subunit...
  31. ROLES OF DNA PK IN DNA DOUBLE STRAND BREAK REPAIR
    David Chen; Fiscal Year: 2001
    ..e., V(D)J recombination. These three proteins are Ku70, Ku80 and DNAPKcs (460 kDa)...
  32. Function domains of Ku proteins as molecular targets
    Fanqing Chen; Fiscal Year: 2003
    ..Specifically, we will focus on the Ku70 and Ku80 proteins (Ku70/80 heterodimers), which are critical components in the repair of ionizing radiation-induced ..
  33. GENETIC VARIATION IN HUMAN NHEJ DNA REPAIR GENES
    Carl Anderson; Fiscal Year: 2005
    ..at least five proteins that appear to be unique to the NHEJ mechanism, the three components of DNA-PK (DNA-PKcs, Ku70, Ku80), the double-stranded DNA activated protein kinase, and DNA ligase IV and XRCC4...
  34. DEAF-1 Interactions and Protein Modifications in Prostate Cells
    Jodi Huggenvik; Fiscal Year: 2009
    ..DEAF-1 interacts with proteins associated with apoptosis or cell survival (Ku70, p53 and HSP90), and DEAF-1 is increased and/or stabilized in the human prostate cancer cell line PC3 following ..
  35. High Resolution Gold Labels for EM
    James Hainfeld; Fiscal Year: 2007
    ..positions in several important protein complexes: The DNA repair complexes consisting of subcomplexes: human Ku70/Ku80/DNA-PK complex, human ligase IV/Xrcc4 complex, human RAD50/Mre11/Nbs1 complex, and the human chromatin ..
  36. KARP 1 V(D)J RECOMBINATION AND DNA REPAIR
    ERIC HENDRICKSON; Fiscal Year: 2001
    ..In particular, the DNA-dependent protein kinase catalytic subunit, Ku86, Ku70, XR-1 and DNA ligase IV gene products all appear to be required for both of these processes...
  37. Mouse Model for Chemical and Radiation Mutagenesis
    Jay Tischfield; Fiscal Year: 2006
    ..5. To introduce Ku70 deficiency to test whether or not NHEJ and HR have overlapping functions. 6...
  38. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    BRENDA SALERNO; Fiscal Year: 2006
    ..IV/XRCC4 complex and the DNA-dependent Protein Kinase (DNA-PK), which is formed by DNA-PKcs, and the heterodimer Ku70/80...
  39. XIP8, AN X RAY INDUCIBLE KU70 BINDING PROTEIN
    David Boothman; Fiscal Year: 2001
    ..It is hypothesized that the induction and overexpression of the nuclear form of XIP8/TRPM-2/ApoJ after IR inhibits Ku70/Ku8O DNA end-binding, ATPase and/or helicase functions, resulting in loss of DNA- PK activity and nonhomologous DSB ..
  40. NMR Studies of Ku70 and Ku80 Domains
    Yuan Chen; Fiscal Year: 2005
    ..The proteins that are central to the major pathway of double-strand-break repair process are Ku70 (69.8 kD) and Ku80 (82.7 kD)...
  41. Transgenic Mouse for DNA Damage Sensing and Signaling
    David Chen; Fiscal Year: 2005
    ..We have generated in the lab or have acquired mice that are knockouts for Ku70, Ku80, DNA-PKcs, Ku80/DNA-PKcs, and ATM...
  42. Mechanisms of Cellular Transduction with AAV Vectors
    Daniel Miller; Fiscal Year: 2006
    ..Finally, we plan to disrupt genes involved in homologous recombination and DSBR (Rad52, Rad54, and Ku70) using homologous integration of AAV vectors and define cellular pathways involved in homologous and non-homologous ..
  43. Granzyme a Pathway of CTL-Mediated Cell Death
    Judy Lieberman; Fiscal Year: 2008
    ..abstract_text> ..
  44. HIV FITNESS, VIRULENCE AND EVOLUTION
    Warner Greene; Fiscal Year: 2003
    ....
  45. CD8 CELL DYSFUNCTION IN HIV INFECTION
    Judy Lieberman; Fiscal Year: 2005
    ..In vitro experiments will be performed to dissect the relative importance of signaling and perforin defects in impaired HIV-specific cytotoxicity and to explore what factors regulate and can reverse impaired CD8 T cell function. ..
  46. Exploring How HTLV-1 Tax Induces T Cell Leukemia
    Warner Greene; Fiscal Year: 2006
    ..Through these combined in vivo and in vitro experiments, we hope to gain key insights into the molecular events underlying HTLV-I Tax mediated leukemogenesis. ..
  47. DNA PK IN RADIATION DAMAGE REPAIR AND LYMPHOMAGENESIS
    Gloria Li; Fiscal Year: 2002
    ..of a 465-kDA catalytic subunit, DNA-PKcs, and a 70 kDA and an 86-kDa heterodimeric DNA-targeting component, Ku70 and Ku80...
  48. Mechanism of DNA Damage Recognition in Higher Eukaryotes
    Suk Hee Lee; Fiscal Year: 2005
    ..Various mutants of damage recognition factors (RPA and XPC) will be used to examine any unique role these proteins possess in 3' and 5' incising. ..
  49. FUNCTION OF PCNA IN CELLULAR DNA REPAIR
    Yoshihiro Matsumoto; Fiscal Year: 2001
    ..abstract_text> ..
  50. Microglia and the Neuropathogenesis of HIV Disease
    Warner Greene; Fiscal Year: 2006
    ..This work should both define the utility of this new experimental system for studying neuropathogenesis and provide new information about the pathologic events that may contribute to impairment of neurologic function in HIV disease. ..
  51. Molecular Mechanisms in the Treatment of Neuroblastoma
    Valerie Castle; Fiscal Year: 2005
    ..abstract_text> ..
  52. OXIDATIVE STRESS, APOPTOSIS AND DRUG RESISTANCE
    Istvan Boldogh; Fiscal Year: 2003
    ..abstract_text> ..
  53. HYPERTHERMIA AND THE FUNCTION OF HSP70
    Gloria Li; Fiscal Year: 2007
    ..which Ku suppresses heat-induction of hsp70 We will test a hypothesis, inferred from our preliminary studies, that Ku70 overexpression leads to hyper- methylation of CpGs within the hsp70 promoter, and that this hypermethylation in ..
  54. MOLECULAR MECHANISMS OF NHEJ IN MAMMALIAN CELLS
    LESLYN HANAKAHI; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  55. Regulation and Action of APOBEC3G
    Warner Greene; Fiscal Year: 2009
    ..With such understanding, new therapeutic strategies for inhibiting HIV growth could emerge. ..
  56. ANALYSIS OF THE ROLE OF VPR IN HIV PATHOGENESIS
    Warner Greene; Fiscal Year: 2004
    ..abstract_text> ..
  57. Signal Transduction by Cytokine Receptors in Vivo
    Warner Greene; Fiscal Year: 2004
    ..The findings should also point to new, rational therapeutic avenues for reversing these conditions in some settings. ..
  58. ROLE OF CD30: CD153-PATHOGENESIS OF RHEUMATOID ARTHRITIS
    Andrea Cerutti; Fiscal Year: 2003
    ..In addition to elucidating the pathogenesis of RA, the experiments outlined in this application should allow to devise new strategies aimed at reducing the production of pathogenic antibodies, including rheumatoid factors. ..
  59. The Role of the Mre11 Complex in Heat-radiosensitization
    JOSEPH DYNLACHT; Fiscal Year: 2007
    ..We will also determine the role of HR in heat-radiosensitization using silencing RNA (siRNA) targeting technology or conditional mutants deficient in specific NHEJ or HR proteins. [unreadable] [unreadable]..
  60. MECHANISM OF BASE EXCISION REPAIR IN HIGHER EUKARYOTES
    Yoshihiro Matsumoto; Fiscal Year: 2003
    ..However, since the 5'-incised AP site is a unique DNA structure in this repair system, this study will add a new insight to the functions of these versatile proteins. ..
  61. DNA REPAIR/V(D)J RECOMBINATION--FUNCTIONAL STUDY
    Guillermo Taccioli; Fiscal Year: 2001
    ....