ICAT

Summary

Gene Symbol: ICAT
Description: catenin beta interacting protein 1
Alias: ICAT, beta-catenin-interacting protein 1, beta-catenin-interacting protein ICAT, inhibitor of beta-catenin and Tcf-4
Species: human

Top Publications

  1. ncbi IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli
    Manisha Sharma
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute at Westmead Hospital, Westmead, New South Wales 2145, Australia
    J Biol Chem 282:8545-56. 2007
  2. ncbi The crystal structure of the beta-catenin/ICAT complex reveals the inhibitory mechanism of ICAT
    Thomas A Graham
    Department of Biological Structure, University of Washington, Seattle, WA 98195, USA
    Mol Cell 10:563-71. 2002
  3. ncbi Mutation and expression of the beta-catenin-interacting protein ICAT in human colorectal tumors
    Toru Koyama
    Second Department of Surgery, Gunma University School of Medicine, Maebashi, Laboratory of Molecular and Genetic Information, Institution of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    Jpn J Clin Oncol 32:358-62. 2002
  4. ncbi Role for ICAT in beta-catenin-dependent nuclear signaling and cadherin functions
    Cara J Gottardi
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908 0732, USA
    Am J Physiol Cell Physiol 286:C747-56. 2004
  5. ncbi Mascot file parsing and quantification (MFPaQ), a new software to parse, validate, and quantify proteomics data generated by ICAT and SILAC mass spectrometric analyses: application to the proteomics study of membrane proteins from primary human endothelia
    David Bouyssie
    Laboratoire de Protéomique et Spectrométrie de Masse des Biomolécules, Equipe Labellisée LIGUE 2006, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Toulouse, France
    Mol Cell Proteomics 6:1621-37. 2007
  6. doi Metadegradomics: toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteome
    Alain Doucet
    Centre for Blood Research, 4 401 Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 7:1925-51. 2008
  7. ncbi Identification of thioredoxin disulfide targets using a quantitative proteomics approach based on isotope-coded affinity tags
    Per Hägglund
    Enzyme and Protein Chemistry, Department of Systems Biology, Søltofts Plads, Building 224, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Proteome Res 7:5270-6. 2008
  8. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
  9. doi Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancers
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington, USA
    OMICS 13:345-54. 2009
  10. pmc Chronic ethanol feeding affects proteasome-interacting proteins
    Marie Pierre Bousquet-Dubouch
    CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Proteomics 9:3609-22. 2009

Detail Information

Publications196 found, 100 shown here

  1. ncbi IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli
    Manisha Sharma
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millennium Institute at Westmead Hospital, Westmead, New South Wales 2145, Australia
    J Biol Chem 282:8545-56. 2007
    ....
  2. ncbi The crystal structure of the beta-catenin/ICAT complex reveals the inhibitory mechanism of ICAT
    Thomas A Graham
    Department of Biological Structure, University of Washington, Seattle, WA 98195, USA
    Mol Cell 10:563-71. 2002
    ..We have determined the crystal structure at 2.5 A resolution of a complex between beta-catenin and ICAT, a protein that prevents the interaction between beta-catenin and Tcf/Lef family transcription factors...
  3. ncbi Mutation and expression of the beta-catenin-interacting protein ICAT in human colorectal tumors
    Toru Koyama
    Second Department of Surgery, Gunma University School of Medicine, Maebashi, Laboratory of Molecular and Genetic Information, Institution of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
    Jpn J Clin Oncol 32:358-62. 2002
    ..We have recently identified the ICAT gene, which encodes a small protein that interacts with beta-catenin and represses Wnt signaling.
  4. ncbi Role for ICAT in beta-catenin-dependent nuclear signaling and cadherin functions
    Cara J Gottardi
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908 0732, USA
    Am J Physiol Cell Physiol 286:C747-56. 2004
    Inhibitor of beta-catenin and TCF-4 (ICAT) is a 9-kDa polypeptide that inhibits beta-catenin nuclear signaling by binding beta-catenin and competing its interaction with the transcription factor TCF (T cell factor), but basic ..
  5. ncbi Mascot file parsing and quantification (MFPaQ), a new software to parse, validate, and quantify proteomics data generated by ICAT and SILAC mass spectrometric analyses: application to the proteomics study of membrane proteins from primary human endothelia
    David Bouyssie
    Laboratoire de Protéomique et Spectrométrie de Masse des Biomolécules, Equipe Labellisée LIGUE 2006, Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Toulouse, France
    Mol Cell Proteomics 6:1621-37. 2007
    ..search engine and performs protein quantification in the case of isotopic labeling experiments using either the ICAT or SILAC (stable isotope labeling with amino acids in cell culture) method...
  6. doi Metadegradomics: toward in vivo quantitative degradomics of proteolytic post-translational modifications of the cancer proteome
    Alain Doucet
    Centre for Blood Research, 4 401 Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Mol Cell Proteomics 7:1925-51. 2008
    ..Quantitative proteomics using stable isotope labeling, such as ICAT, isobaric tags for relative and absolute quantification (iTRAQ), and stable isotope labeling by amino acids in cell ..
  7. ncbi Identification of thioredoxin disulfide targets using a quantitative proteomics approach based on isotope-coded affinity tags
    Per Hägglund
    Enzyme and Protein Chemistry, Department of Systems Biology, Søltofts Plads, Building 224, Technical University of Denmark, DK 2800 Kgs Lyngby, Denmark
    J Proteome Res 7:5270-6. 2008
    ..reduction based on thiol-specific differential labeling with the iodoacetamide-based isotope-coded affinity tag (ICAT) reagents...
  8. ncbi Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol
    Keith Vosseller
    Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Proteomics 5:388-98. 2005
    ..BEMAD was comparable to isotope-coded affinity tags (ICAT; a commercially available differential isotopic quantification technique) in protein expression analysis, but also ..
  9. doi Quantitative proteomics analysis integrated with microarray data reveals that extracellular matrix proteins, catenins, and p53 binding protein 1 are important for chemotherapy response in ovarian cancers
    Sheng Pan
    Department of Pathology, University of Washington, Seattle, Washington, USA
    OMICS 13:345-54. 2009
    ..We applied a quantitative proteomic approach based on ICAT/MS/MS technology to analyze tissues harvested at primary debulking surgery before the initiation of combination ..
  10. pmc Chronic ethanol feeding affects proteasome-interacting proteins
    Marie Pierre Bousquet-Dubouch
    CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Proteomics 9:3609-22. 2009
    ..b>ICAT and MS/MS spectral counting, further confirmed by Western blot, showed that the levels of several PIPs were ..
  11. ncbi [Effect of C/EBPalpha on the monocytic differentiation of HL60 cells induced by NSC67657]
    Wei Jia Wang
    Department of Laboratory Medicine, Zhongshan People s Hospital, The Affiliated Hospital of Zhongshan University, Zhongshan 528402, China
    Zhonghua Zhong Liu Za Zhi 32:262-6. 2010
    ..To figure out the function of C/EBPalpha in the monocytic differentiation of HL60 cells induced by a new steroidal drug NSC67657...
  12. pmc Alpha 1-adrenoceptor activation of a non-selective cation current in rabbit portal vein by 1,2-diacyl-sn-glycerol
    R M Helliwell
    Department of Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, London, UK
    J Physiol 499:417-28. 1997
    ..transduction mechanisms involved in the activation and modulation of the noradrenaline-activated cation current (Icat) were investigated with whole-cell patch clamp techniques in rabbit portal vein smooth muscle cells. 2...
  13. ncbi ANIBAL, stable isotope-based quantitative proteomics by aniline and benzoic acid labeling of amino and carboxylic groups
    Alexandre Panchaud
    Département de Microbiologie Fondamentale, Universite de Lausanne, Quartier UNIL Sorge, Bâtiment Biophore, CH 1015 Lausanne, Switzerland
    Mol Cell Proteomics 7:800-12. 2008
    ..Although the combination of existing approaches, e.g. ICAT with isotope-coded protein labeling, is analytically feasible, it implies high costs, and the combined application ..
  14. pmc Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker
    Un Beom Kang
    Life Sciences Division, Korea Institute of Science and Technology, Seoul 136 791, Korea
    BMC Cancer 10:114. 2010
    ..It is important to discover a reliable biomarker for the detection of breast cancer. Plasma is the most ideal source for cancer biomarker discovery since many cells cross-communicate through the secretion of soluble proteins into blood...
  15. ncbi A method to identify and simultaneously determine the relative quantities of proteins isolated by gel electrophoresis
    Salvatore Sechi
    National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 6707 Democracy Blvd, Rm 611, Bethesda, MD 20892 5460, USA
    Rapid Commun Mass Spectrom 16:1416-24. 2002
    ..g. using ICAT reagents or growing cells in isotopically enriched nutrients)...
  16. pmc Proteomic analysis of nipple aspirate fluid from women with early-stage breast cancer using isotope-coded affinity tags and tandem mass spectrometry reveals differential expression of vitamin D binding protein
    Timothy M Pawlik
    Department of Surgical Oncology, The University of Texas M, d, Anderson Cancer Center, Houston, Texas, USA
    BMC Cancer 6:68. 2006
    Isotope-coded affinity tag (ICAT) tandem mass spectrometry (MS) allows for qualitative and quantitative analysis of paired protein samples...
  17. doi Quantitative proteomics for identification of cancer biomarkers
    Raghothama Chaerkady
    Institute of Bioinformatics, International Technology Park, Bangalore, India Departments of Biological Chemistry, Pathology and Oncology, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, MD, USA
    Proteomics Clin Appl 1:1080-9. 2007
    ..methods such as stable isotope labeling with amino acids in cell culture (SILAC), isotope-coded affinity tagging (ICAT), isobaric tags for relative and absolute quantitation (iTRAQ), and (18) O labeling have all been used in ..
  18. ncbi Protease degradomics: mass spectrometry discovery of protease substrates and the CLIP-CHIP, a dedicated DNA microarray of all human proteases and inhibitors
    Christopher M Overall
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Biol Chem 385:493-504. 2004
    ..In another approach we used isotope-coded affinity tag (ICAT) labeling with tandem mass spectrometry to quantitate the levels of secreted or shed extracellular proteins in MDA-..
  19. ncbi Interagency relationships among rural early intervention collaboratives
    B J Polivka
    Ohio State University College of Nursing, Columbus, 43210, USA
    Public Health Nurs 18:340-9. 2001
    ..Respondents from 42 agencies completed the 18-item Interagency Collaboration Assessment Tool (ICAT) and demographic questions. The ICAT uses a 5-point Likert-type scale to assess the five domains of the model...
  20. ncbi Angiotensin II signaling pathways mediate expression of cardiac T-type calcium channels
    Laurent Ferron
    CNRS UMR 8078, Remodelage Tissulaire et Fonctionnel, Hopital Marie Lannelongue, 133 avenue de la Resistance, 92350 Le Plessis Robinson, France
    Circ Res 93:1241-8. 2003
    Recent studies indicate that cardiac T-type Ca2+ current (ICaT) reappears in hypertrophied ventricular cells...
  21. doi A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems
    Omid Hekmat
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    J Proteome Res 7:3282-92. 2008
    An activity-based isotope-coded affinity tagging (AB-ICAT) strategy for proteome-wide quantitation of active retaining endoglycosidases has been developed...
  22. pmc Elucidation of thioredoxin target protein networks in mouse
    Cexiong Fu
    Center for Advanced Proteomics Research, Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School Cancer Center, Newark, New Jersey 07103, USA
    Mol Cell Proteomics 8:1674-87. 2009
    ..In this study, we performed an ICAT proteomics study for the identification of Trx1 target proteins from the hearts of a cardiac specific Trx1-..
  23. doi Apparent autosomal dominant keratoconus in a large Australian pedigree accounted for by digenic inheritance of two novel loci
    Kathryn P Burdon
    Department of Ophthalmology, Flinders University, Flinders Medical Centre, Bedford Park, Adelaide, SA, 5042, Australia
    Hum Genet 124:379-86. 2008
    ..Although the pedigree appears to demonstrate simple autosomal dominant inheritance, the disorder is actually genetically complex. This pedigree may provide a link between inherited forms of keratoconus and sporadic cases...
  24. pmc Comparative studies of early liver dysfunction in senescence-accelerated mouse using mitochondrial proteomics approaches
    Yashu Liu
    The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Mol Cell Proteomics 7:1737-47. 2008
    ..Here we used both two-dimensional electrophoresis- and ICAT-based mitochondrial proteomics analysis to view the liver mitochondrial protein alterations between SAMP8 and SAMR1...
  25. ncbi Potentiating actions of lanthanum on ACh-induced cation current in guinea-pig ileal smooth muscle cells
    R Inoue
    Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    J Smooth Muscle Res 34:69-81. 1998
    Potentiating actions of external lanthanum (La3+) on muscarinic receptor-activated nonselective cation current (Icat) were investigated in myocytes dissociated from the longitudinal muscle layer of guinea-pig ileum, with a whole-cell ..
  26. pmc Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding
    Georgina S Butler
    Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, Canada
    Mol Cell Biol 28:4896-914. 2008
    ..and membrane fractions with levels that were modulated by the MMPI were identified by isotope-coded affinity tag (ICAT) labeling and tandem mass spectrometry...
  27. ncbi Functional Ca2+ and Na+ channels on mouse Schwann cells cultured in serum-free medium: regulation by a diffusible factor from neurons and by cAMP
    C Beaudu-Lange
    INSERM U394, I F R 8, Bordeaux, France
    Eur J Neurosci 10:1796-809. 1998
    ..Of the Schwann cells that did not contact axons, 46.5% expressed T-type Ca2+ conductances (ICaT)...
  28. pmc Beta-catenin binds to the activation function 2 region of the androgen receptor and modulates the effects of the N-terminal domain and TIF2 on ligand-dependent transcription
    Liang Nian Song
    Department of Oncology, Lombardi Cancer Center, Georgetown University School of Medicine, Washington, D C 20007 2197, USA
    Mol Cell Biol 23:1674-87. 2003
    ..b>ICAT, an inhibitor of T-cell factor 4 (TCF-4), and E-cadherin binding to beta-catenin also blocked binding of the ..
  29. ncbi A comparative evaluation of software for the analysis of liquid chromatography-tandem mass spectrometry data from isotope coded affinity tag experiments
    Robert Moulder
    Turku Centre for Biotechnology, University of Turku and Abo Akademi University, 20521 Turku, Finland
    Proteomics 5:2748-60. 2005
    The options available for processing quantitative data from isotope coded affinity tag (ICAT) experiments have mostly been confined to software specific to the instrument of acquisition...
  30. ncbi Comprehensive proteomic analysis of protein changes during platelet storage requires complementary proteomic approaches
    Jonathan N Thon
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada
    Transfusion 48:425-35. 2008
    ..This study addresses relative differences among proteomics approaches to the analysis of the PLT storage lesion...
  31. ncbi [Quantitative analysis of nuclear proteome in apoptosis hepatoma cells induced by hydroxycamptothecin]
    Yu Rong Yan
    Chongqing University of Medical Science, Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing 400016
    Fen Zi Xi Bao Sheng Wu Xue Bao 41:120-8. 2008
    ..Nuclear proteins labelled with cleavable isotope-coded affinity tag (c-ICAT) reagent were digested and purified...
  32. pmc MaXIC-Q Web: a fully automated web service using statistical and computational methods for protein quantitation based on stable isotope labeling and LC-MS
    Chih Chiang Tsou
    Institute of Information Science and Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan 115
    Nucleic Acids Res 37:W661-9. 2009
    ..g. SILAC, ICAT and user-developed labeling methods...
  33. doi An online lifestyle diary with a persuasive computer assistant providing feedback on self-management
    Olivier A Blanson Henkemans
    Delft University of Technology, Delft, The Netherlands
    Technol Health Care 17:253-67. 2009
    ..e., the use of an online lifestyle diary called DieetInzicht.nl. The assistant is represented by an animated iCat, which shows different facial expressions and provides cooperative feedback following principles from the ..
  34. pmc Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro
    A V Tutter
    Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Genes Dev 15:3342-54. 2001
    ..LEF-1-beta-cat transactivation in vitro was also repressed by inhibitor of beta-catenin and Tcf-4 (ICAT), a physiological inhibitor of Wnt/Wg signaling that interacts with ARM repeats 11 and 12, and by the nonsteroidal ..
  35. ncbi The study of macromolecular complexes by quantitative proteomics
    Jeffrey A Ranish
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103 8904, USA
    Nat Genet 33:349-55. 2003
    ..It is based on the use of isotope-coded affinity tag (ICAT) reagents and mass spectrometry to compare the relative abundances of tryptic peptides derived from suitable pairs ..
  36. ncbi Methods for proteomics in neuroscience
    Nilesh S Tannu
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Prog Brain Res 158:41-82. 2006
    ..g., two-dimensional difference in gel electrophoresis (2D-DIGE), isotope-coded affinity tag (ICAT) and iTRAQ) are elaborated...
  37. ncbi Genomic and proteomic expression analysis of Leishmania promastigote and amastigote life stages: the Leishmania genome is constitutively expressed
    Kirk Leifso
    Immunity and Infection Research Centre, Vancouver Coastal Health Research Institute, Canada
    Mol Biochem Parasitol 152:35-46. 2007
    ..Quantitative proteomics, using the isotope coded affinity tag (ICAT) technology and mass spectrometry, was used to identify L...
  38. pmc Quantification of oxidative posttranslational modifications of cysteine thiols of p21ras associated with redox modulation of activity using isotope-coded affinity tags and mass spectrometry
    Mahadevan Sethuraman
    Vascular Biology Unit, Boston University School of Medicine, Boston, MA 02118, USA
    Free Radic Biol Med 42:823-9. 2007
    ..Here we used an isotope-coded affinity tag (ICAT) and mass spectrometry to quantitate the reversible and irreversible oxidative posttranslational thiol ..
  39. pmc Proteomics analysis of the expression of neurogranin in murine neuroblastoma (Neuro-2a) cells reveals its involvement for cell differentiation
    Nian Lin Reena Han
    Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
    Int J Biol Sci 3:263-73. 2007
    ..changes due to the expression of Ng in murine neuroblastoma (Neuro-2a) cells using isotope coded affinity tags (ICAT) combined with 2-dimensional liquid chromatography/tandem mass spectrometry (2D-LC/MS/MS)...
  40. doi Accuracy of reconstructed images from cone-beam computed tomography scans
    Manish Lamichane
    Department of Developmental Biology, School of Dental Medicine, Harvard University, Boston, Mass
    Am J Orthod Dentofacial Orthop 136:156.e1-6; discussion 156-7. 2009
    ..this study was to determine whether 2-dimensional (2D) images produced from cone-beam computed tomography (CBCT) images taken with an iCAT scanner (Imaging Sciences International, Hatfield, Pa) can substitute for traditional cephalograms.
  41. doi Automated generic analysis tools for protein quantitation using stable isotope labeling
    Wen Lian Hsu
    Institute of Information Science, Academia Sinica, Taipei, Taiwan
    Methods Mol Biol 604:257-72. 2010
    ..g., iTRAQ-labeling quantitation, and the other by MS scans, e.g., quantitation using SILAC, ICAT, or (18)O labeling...
  42. pmc Proteomic profiling of lipopolysaccharide-activated macrophages by isotope coded affinity tagging
    Kristian E Swearingen
    Department of Chemistry, University of Washington, P O Box 351700, Seattle, Washington 98195 1700, USA
    J Proteome Res 9:2412-21. 2010
    ..We performed isotope coded affinity tagging (ICAT), multidimensional liquid chromatography, and mass spectrometry to identify proteins that are differently expressed ..
  43. ncbi Wheat proteomics: relationship between fine chromosome deletion and protein expression
    Nazrul Islam
    Kihara Institute for Biological Research, Yokohama City University, Japan
    Proteomics 3:307-16. 2003
    ..to chromosome deletion, the feasibility of a new analytical approach based on isotope coded affinity tag labeling (ICAT) of peptides in tryptic digests followed by electrospray ionization mass spectrometry has been described...
  44. ncbi Minimizing resolution of isotopically coded peptides in comparative proteomics
    Roujian Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    J Proteome Res 1:139-47. 2002
    ..Heavy isotope derivatizing agents used in this work were the commercially available 2H8-ICAT reagent and 13C4-succinic anhydride...
  45. ncbi Evaluation of shotgun sequencing for proteomic analysis of human plasma using HPLC coupled with either ion trap or Fourier transform mass spectrometry
    Shiaw Lin Wu
    ThermoFinnigan, 355 River Oaks Parkway, San Jose, California, USA
    J Proteome Res 2:383-93. 2003
    ..The isotope coded affinity tag (ICAT) approach was used to quantitate successfully different levels of hGH in replicate analysis via the disulfide ..
  46. pmc Synergism between inositol phosphates and diacylglycerol on native TRPC6-like channels in rabbit portal vein myocytes
    A P Albert
    Department of Basic Medical Sciences, Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
    J Physiol 552:789-95. 2003
    In rabbit portal vein myocytes noradrenaline activates a non-selective cation current (Icat) which involves a transient receptor potential protein (TRPC6)...
  47. ncbi Quantitative proteomic and transcriptional analysis of the response to the p38 mitogen-activated protein kinase inhibitor SB203580 in transformed follicular lymphoma cells
    Zhaosheng Lin
    Associated Regional and University Pathologists ARUP Institute for Clinical and Experimental Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Mol Cell Proteomics 3:820-33. 2004
    ..Quantitative proteomic analysis using ICAT-LC-MS/MS identified 277 differentially expressed proteins at 3 h and 350 proteins at 21 h of treatment with ..
  48. ncbi Web-accessible proteome databases for microbial research
    Klaus Peter Pleissner
    Max Planck Institute for Infection Biology, Berlin, Germany
    Proteomics 4:1305-13. 2004
    ..Novel technologies such as isotope coded affinity tag (ICAT)-liquid chromatography/mass spectrometry (LC/MS) open new insights into protein alterations...
  49. ncbi L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes
    K Zorn-Pauly
    Institut für Mediziniche Physik und Biophysik, Universitat Graz, Harrachgasse 21, A 8010 Graz, Austria
    Physiol Res 53:369-77. 2004
    ..Using whole-cell patch clamp techniques ICaL and ICaT were studied by applying voltage protocols from different holding potentials (-40 and -90 mV)...
  50. ncbi Quantitative proteomics of cerebrospinal fluid from patients with Alzheimer disease
    Jing Zhang
    Department of Pathology, University of Washington School of Medicine, Seattle, WA 98104, USA
    J Alzheimers Dis 7:125-33; discussion 173-80. 2005
    ..microcapillary liquid chromatography mass spectrometry of proteins labeled with isotope-coded affinity tags (ICAT), to quantify relative changes in the proteome of human cerebrospinal fluid (CSF) obtained from the lumbar cistern...
  51. ncbi In vivo drug-response in patients with leukemic non-Hodgkin's lymphomas is associated with in vitro chemosensitivity and gene expression profiling
    Kai Uwe Chow
    University Hospital, Department of Internal Medicine II, Hematology and Oncology, Frankfurt Main, Germany
    Pharmacol Res 53:49-61. 2006
    ..and cell cycle signalling including complement lysis inhibitor (clusterin/CLU), beta-catenin interacting protein (ICAT), peroxisome proliferator activated receptor alpha (PPARalpha), TNF alpha converting enzyme (ADAM17/TACE), homeo ..
  52. pmc High-throughput identification of IMCD proteins using LC-MS/MS
    Trairak Pisitkun
    Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Physiol Genomics 25:263-76. 2006
    ..We used the isotope-coded affinity tag (ICAT) technique to identify IMCD proteins that change in abundance in response to vasopressin...
  53. ncbi Label-free protein quantification using LC-coupled ion trap or FT mass spectrometry: Reproducibility, linearity, and application with complex proteomes
    Guanghui Wang
    Proteomics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Proteome Res 5:1214-23. 2006
    ..While stable-isotope labeling (e.g., ICAT, 18O- or 15N-labeling, or AQUA) remains the core technology used in mass spectrometry-based proteomic ..
  54. ncbi Quantitative proteomic profiling of pancreatic cancer juice
    Ru Chen
    GI Division Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Proteomics 6:3871-9. 2006
    ..We used isotope-code affinity tag (ICAT) technology and MS/MS to perform quantitative protein profiling of pancreatic juice from pancreatic cancer patients ..
  55. ncbi Proteomic analysis of opsins and thyroid hormone-induced retinal development using isotope-coded affinity tags (ICAT) and mass spectrometry
    W Ted Allison
    Department of Biology, University of Victoria, Victoria, British Columbia, Canada
    Mol Vis 12:655-72. 2006
    ..We also sought to begin a characterization of proteomic changes in the retina occurring with TH treatment and address whether TH affects proliferation or photoreceptor differentiation...
  56. ncbi Protein profiling of sickle cell versus control RBC core membrane skeletons by ICAT technology and tandem mass spectrometry
    Jose Chou
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas, USA
    Cell Mol Biol Lett 11:326-37. 2006
    A proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and determine ..
  57. ncbi Morphine administration alters the profile of hippocampal postsynaptic density-associated proteins: a proteomics study focusing on endocytic proteins
    Jose A Morón
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Cell Proteomics 6:29-42. 2007
    ..We applied a quantitative proteomics analysis technique with the cleavable ICAT reagent to quantitate relative changes in protein levels of the hippocampal PSD in response to morphine ..
  58. ncbi Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling
    Michael B Major
    Howard Hughes Medical Institute, University of Washington School of Medicine, Box 357370, Seattle, WA 98195, USA
    Science 316:1043-6. 2007
    ..These data provide a possible mechanistic explanation for the tumor suppressor activity of WTX...
  59. ncbi Loss of ICAT gene function leads to arrest of ureteric bud branching and renal agenesis
    Yoshimi Hasegawa
    Department of Anatomy I, Fujita Health University School of Medicine, Toyoake, Aichi 470 1192, Japan
    Biochem Biophys Res Commun 362:988-94. 2007
    b>ICAT, inhibitor of beta-catenin and T cell factor, or Ctnnbip1, is a negative regulator of the Wnt signaling pathway that interferes with the interaction between beta-catenin and T cell factor...
  60. pmc Differential proteomics in the aging Noble rat ventral prostate
    Ying Wai Lam
    Department of Environmental Health, Division of Environmental Genetics and Molecular Toxicology, University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Proteomics 8:2750-63. 2008
    ..of Noble rats by identifying differentially expressed prostate proteins between 3- and 16-month-old animals using ICAT and MS...
  61. ncbi [17 beta-estradiol inhibits T-type calcium channels in mouse spermatogenic cells]
    Liang Lu
    The Ministry of Education Key Lab of Modern Toxicology, Nanjing Medical University, Nanjing, Jiangsu 210029, China
    Zhonghua Nan Ke Xue 14:483-8. 2008
    To investigate the effect of 17 beta-estradiol (E2) on T-type calcium currents (ICaT) in spermatogenic cells.
  62. doi Proteomic analysis of lymphoid and haematopoietic neoplasms: there's more than biomarker discovery
    Alberto Zamo
    Dip di Patologia, Sezione di Anatomia Patologica, Universita di Verona, Verona, Italy
    J Proteomics 73:508-20. 2010
    ..and tissues proteomics, and discuss the improvement allowed by the new developed proteomic strategies, such as nanofluidic systems, analysis of formalin-fixed tissues, and quantitative high throughput techniques (SILAC, ICAT, iTRAQ).
  63. doi Quantitative proteomic analysis of CMS-related changes in Honglian CMS rice anther
    Qingping Sun
    Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, People s Republic of China
    Protein J 28:341-8. 2009
    ..We used isotope-code affinity tag (ICAT) technology to perform the protein profiling of uninucleate stage rice anther and identify the CMS-HL related ..
  64. doi MSQuant, an open source platform for mass spectrometry-based quantitative proteomics
    Peter Mortensen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Center for Experimental Bioinformatics, Odense, Campusvej 55, DK 5230 Odense M, Denmark
    J Proteome Res 9:393-403. 2010
    ..g., (15)N), residue labels (e.g., SILAC and ICAT), termini labels (e.g., (18)O), functional group labels (e.g., mTRAQ), and label-free ion intensity approaches...
  65. doi Thrombus of the internal carotid artery: the contribution of computed tomographic angiography to clinical management
    George T Pisimisis
    Division of Vascular Surgery, Mayo Clinic, Rochester, MN, USA
    Vasc Endovascular Surg 44:302-4. 2010
    ..We describe 2 symptomatic patients with severe stenosis on duplex scanning and internal carotid artery thrombus (ICAT) identified on subsequent computed tomographic angiography...
  66. pmc A coactivator role of CARM1 in the dysregulation of β-catenin activity in colorectal cancer cell growth and gene expression
    Chen Yin Ou
    Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, CA, USA
    Mol Cancer Res 9:660-70. 2011
    ....
  67. doi Cone-beam computed tomography evaluation of relationship between tongue volume and lower incisor irregularity
    Tancan Uysal
    Department of Orthodontics, Faculty of Dentistry, Izmir Katip Celebi University, Izmir, Turkey
    Eur J Orthod 35:555-62. 2013
    ..Tomography was carried out using iCAT® (Imaging Sciences International, Hatfield, Pennsylvania, USA) and segmentation was carried out by using Mimics 10...
  68. ncbi Expression of E-cadherin and β-catenin in two cholangiocarcinoma cell lines (OZ and HuCCT1) with different degree of invasiveness of the primary tumor
    Yasser Abuetabh
    Department of Lab Medicine and Pathology, University of Alberta Hospital, NW, Edmonton, Alberta T6G 2B7, Canada
    Ann Clin Lab Sci 41:217-23. 2011
    ....
  69. doi Cost-effectiveness and cost-utility of beclomethasone/formoterol versus fluticasone propionate/salmeterol in patients with moderate to severe asthma
    Simone Gerzeli
    Department of Political and Social Sciences, University of Pavia, Pavia, Italy
    Clin Drug Investig 32:253-65. 2012
    ..However, this study did not provide evidence on costs and did not quantify quality-of-life parameters...
  70. doi Development of retinal pigment epithelium from human parthenogenetic embryonic stem cells and microRNA signature
    Wen bo Li
    Tianjin Medical University Eye Centre, Tianjin, China
    Invest Ophthalmol Vis Sci 53:5334-43. 2012
    ..We investigated the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into RPE cells, and identified development-regulating microRNAs (miRNAs)...
  71. doi Secretion and maturation of conotoxins in the venom ducts of Conus textile
    Rowan Dobson
    Laboratory of Mass Spectrometry, Department of Chemistry, University of Liege, Liege, Belgium
    Toxicon 60:1370-9. 2012
    ..This research uses the two mass spectrometric techniques of isotope Coded Affinity Tagging (ICAT) and label-free quantification to study each of five portions of the venom duct of Conus textile snails from New ..
  72. doi Beta-catenin and survivin expression in keratocystic odontogenic tumor (KCOT). A comparative immunohistochemical study in primary, recurrent and nevoid basal cell carcinoma syndrome (NBCCS)-associated lesions
    R Leonardi
    Department of Medical and Surgical Science II Dental Unit University of Catania, Catania, Italy
    Histol Histopathol 28:1175-84. 2013
    ....
  73. pmc Identification of thioredoxin target protein networks in cardiac tissues of a transgenic mouse
    Cexiong Fu
    Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, Pfizer Inc, Groton, CT, USA
    Methods Mol Biol 1005:181-97. 2013
    ..techniques-isobaric tags for relative and absolute quantification (iTRAQ) and redox isotope-coded affinity tags (ICAT)-to elucidate the cardiovascular redox-proteome changes and thioredoxin 1 (Trx1)-regulated protein network in ..
  74. pmc Parathyroid hormone-related protein inhibits DKK1 expression through c-Jun-mediated inhibition of β-catenin activation of the DKK1 promoter in prostate cancer
    H Zhang
    Department of Urology, School of Medicine, University of Michigan, Ann Arbor, MI, USA
    Oncogene 33:2464-77. 2014
    ..Together, these results demonstrate a novel signaling linkage between PTHrP and Wnt signaling pathways that results in downregulation of a Wnt inhibitor allowing for Wnt activity that could contribute the osteoblastic nature of PCa. ..
  75. pmc Improved identification of wheat gluten proteins through alkylation of cysteine residues and peptide-based mass spectrometry
    Ine Rombouts
    German Research Center for Food Chemistry, Leibniz Institute, Lise Meitner Strasse 34, D 85354 Freising, Germany
    Sci Rep 3:2279. 2013
    ..In addition, to the best of our knowledge, we were the first to label prolamins with isotope coded affinity tags (ICAT), which are commonly used for quantitative proteomics...
  76. doi Expression of β-catenin and cyclin D1 in Merkel cell carcinomas of the head and neck
    Claudia Lill
    Department of Otorhinolaryngology, Head and Neck Surgery, Medical University of Vienna, Waehringer Guertel 18 20, 1090, Vienna, Austria
    Wien Klin Wochenschr 125:501-7. 2013
    ..The Wnt pathway protein β-catenin and the cell cycle protein cyclin D1 are two promotors of tumor growth and are expressed in a variety of malignant neoplasms such as lymphomas, thyroid, breast cancer, and many others...
  77. doi New perspectives on β-catenin control of cell fate and proliferation in colon cancer
    Yang Yang
    Clinical College of General Hospital of Armed Police Forces, Anhui Medical University, Hefei 230032, China Department of Anorectal, General Hospital of Armed Police Forces, Beijing 100039, China
    Food Chem Toxicol 74:14-9. 2014
    ..These results show that β-catenin may serve as a good diagnosis biomarker of early-stage tumor development and a novel potential therapeutic target for colon cancer. ..
  78. doi Identifying risky drinking patterns over the course of Saturday evenings: An event-level study
    Emmanuel Kuntsche
    Addiction Switzerland, Research Institute
    Psychol Addict Behav 29:744-52. 2015
    ..completed by 164 young adults on 514 evenings via the Internet-based cell phone optimized assessment technique (ICAT). The results showed that the 2-group solution best fitted the data with a "stable low" drinking pattern (64...
  79. doi Simultaneous silencing of β-catenin and signal transducer and activator of transcription 3 synergistically induces apoptosis and inhibits cell proliferation in HepG2 liver cancer cells
    Xin Hong Wang
    Department of Gastroenterology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P R China
    Mol Med Rep 12:2263-8. 2015
    ....
  80. doi Stable isotope labelling methods in mass spectrometry-based quantitative proteomics
    Osama Chahrour
    Spectroscopy Group, Analytical Services, Almac, UK Electronic address
    J Pharm Biomed Anal 113:2-20. 2015
    ..Several different strategies involving stable isotopes label (ICAT, ICPL, IDBEST, iTRAQ, TMT, IPTL, SILAC), label-free statistical assessment approaches (MRM, SWATH) and absolute ..
  81. doi The Isotope-Coded Affinity Tag Method for Quantitative Protein Profile Comparison and Relative Quantitation of Cysteine Redox Modifications
    James Chun Yip Chan
    Department of Pharmacy, National University of Singapore, Singapore
    Curr Protoc Protein Sci 82:23.2.1-19. 2015
    The isotope-coded affinity tag (ICAT) technique has been applied to measure pairwise changes in protein expression through differential stable isotopic labeling of proteins or peptides followed by identification and quantification using a ..
  82. doi Phage Selection of Peptide Macrocycles against β-Catenin To Interfere with Wnt Signaling
    Davide Bertoldo
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne EPFL, 1015, Lausanne, Switzerland
    ChemMedChem 11:834-9. 2016
    ..The binding site of one group of ligands was mapped to the interaction region of the translational Wnt inhibitor ICAT (inhibitor of β-catenin and Tcf), which is a prime target site on β-catenin for therapeutic intervention, and to ..
  83. doi Multicenter Feasibility Study of Tumor Molecular Profiling to Inform Therapeutic Decisions in Advanced Pediatric Solid Tumors: The Individualized Cancer Therapy (iCat) Study
    Marian H Harris
    Department of Pathology, Boston Children s Hospital, Boston, Massachusetts
    JAMA Oncol . 2016
    ..Consequently, it is unknown whether clinical sequencing can be of benefit...
  84. doi Frequent inactivation of MCC/CTNNBIP1 and overexpression of phospho-beta-catenin(Y654) are associated with breast carcinoma: Clinical and prognostic significance
    Nupur Mukherjee
    Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37, S P Mukherjee Road, Kolkata, West Bengal 700026, India Electronic address
    Biochim Biophys Acta 1862:1472-84. 2016
    ..Thus, our data suggests that inactivation of MCC/CTNNBIP1 could be an important event in activation of β-catenin mediated transcription of target genes in BC. ..
  85. pmc A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells
    Annette S Flozak
    Department of Pulmonary Medicine, Feinberg School of Medicine, Northwestern University, 240 East Huron St, McGaw Pavilion, M 323, Chicago, IL, 60611, USA
    Methods Mol Biol 1481:49-60. 2016
    ..allows for simple assessment of the cadherin-free fraction of β-catenin in cells, using a GST-tagged form of ICAT (Inhibitor of β-Catenin and Tcf) as an affinity matrix...
  86. doi Interactive roles of the cerebellum and striatum in sub-second and supra-second timing: Support for an initiation, continuation, adjustment, and termination (ICAT) model of temporal processing
    Elijah A Petter
    Department of Psychology and Neuroscience, Duke University, Durham, NC, USA
    Neurosci Biobehav Rev 71:739-755. 2016
    ....
  87. ncbi Use of Transportable Radiation Detection Instruments to Assess Internal Contamination from Intakes of Radionuclides Part II: Calibration Factors and ICAT Computer Program
    Robert Anigstein
    S Cohen and Associates, 1608 Spring Hill Road, Vienna, VA 2218 HP Consulting, LLC, 20 Grand Canyon Drive, Los Alamos, NM 87544 Radiation Studies Branch, EHHE, NCEH, Centers for Disease Control and Prevention Atlanta, GA 30341 3717
    Health Phys 111:542-558. 2016
    ..These calibration factors also constitute input data to the ICAT computer program, an interactive Microsoft Windows-based software package that estimates intakes of radionuclides ..
  88. pmc CARF activates beta-catenin/TCF signaling in the hepatocellular carcinoma
    Xin Fan
    Department of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, PR China
    Oncotarget 7:80404-80414. 2016
    ..mechanism study, CARF was found to interact with beta-catenin, impaired the interaction between beta-catenin and ICAT, and activated beta-catenin/TCF signaling...
  89. doi A randomized controlled comparison of integrative cognitive-affective therapy (ICAT) and enhanced cognitive-behavioral therapy (CBT-E) for bulimia nervosa
    S A Wonderlich
    Neuropsychiatric Research Institute Department of Clinical Neuroscience, University of North Dakota School of Medicine and Health Sciences, Fargo, ND, USA
    Psychol Med 44:543-53. 2014
    The purpose of this investigation was to compare a new psychotherapy for bulimia nervosa (BN), integrative cognitive-affective therapy (ICAT), with an established treatment, 'enhanced' cognitive-behavioral therapy (CBT-E).
  90. pmc A novel GTP-dependent mechanism of ileal muscarinic metabotropic channel desensitization
    A V Zholos
    Department of Pharmacology and Clinical Pharmacology, St George s Hospital Medical School, London
    Br J Pharmacol 119:997-1005. 1996
    1. Cationic current (Icat) was evoked in single isolated smooth muscle cells either by activating muscarinic receptors with the stable muscarinic agonist, carbachol (CCh), or by dialysing cells with GTP-gamma S...
  91. ncbi The role of proteomics in toxicology: identification of biomarkers of toxicity by protein expression analysis
    Sandy Kennedy
    Oxford GlycoSciences UK Ltd, The Forum, 86 Milton Park, Abingdon, Oxfordshire OX14 4RY, UK
    Biomarkers 7:269-90. 2002
    ..The isotope-coded affinity tag (ICAT) method appears particularly promising...
  92. ncbi Controlling deuterium isotope effects in comparative proteomics
    Roujian Zhang
    Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA
    Anal Chem 74:3662-9. 2002
    ..ester-2H9, [3-(2,5-dioxopyrrolidin-1-yloxycarbonyl)-propyl]-trimethylammonium chloride-2H9, and the commercial ICAT-2H8 reagent...
  93. ncbi Affinity labeling of highly hydrophobic integral membrane proteins for proteome-wide analysis
    Michael B Goshe
    Biological Sciences Division, Pacific Northwest National Laboratory, PO Box 999, MSIN K8 98, Richland, Washington 99352, USA
    J Proteome Res 2:153-61. 2003
    ..08. The protocol described can be used with other stable isotope labeling reagents (e.g., ICAT) to enable comparative measurements to be made on differentially expressed hydrophobic membrane proteins from ..
  94. ncbi Efficient synthesis of isotopically pure isotope-coded affinity tagging reagents
    Anupama Patel
    Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, B C V6T 1Z1 Canada, and PENCE Inc Protein Engineering Network of Centers of Excellence, 750 Heritage Medical Research Center, Edmonton, Alberta T6G 2S2 Canada
    Bioconjug Chem 15:224-30. 2004
    Synthesis of an isotopically pure d8-ICAT linker, N-[(5,5,6,6,8,8,9,9-2H)-13-biotinamido-4,7,10-trioxatridecanyl] tert-butyloxy carbamide (12), has been achieved in seven steps with an overall yield of 33%...
  95. ncbi Proteomic analysis of the intestinal epithelial cell response to enteropathogenic Escherichia coli
    Philip R Hardwidge
    Biotechnology Laboratory, University of British Columbia, Wesbrook Building, 6174 University Boulevard, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 279:20127-36. 2004
    ..Host proteins were isolated and subjected to quantitative profiling using isotope-coded affinity tagging (ICAT) combined with electrospray ionization tandem mass spectrometry...
  96. ncbi Design and synthesis of visible isotope-coded affinity tags for the absolute quantification of specific proteins in complex mixtures
    Patricia Bottari
    Department of Chemistry, University of Washington, Seattle, Washington 98195, USA
    Bioconjug Chem 15:380-8. 2004
    ..We recently introduced isotope-coded affinity tags (ICAT reagents) for the relative quantification of proteins present in two or more biological samples...
  97. ncbi Femtomol sensitivity post-digest (18)O labeling for relative quantification of differential protein complex composition
    Marcus Bantscheff
    Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Rapid Commun Mass Spectrom 18:869-76. 2004
    ..Chemical labeling of complex protein mixtures by, e.g., isotope-coded affinity tags (ICAT), can offer great simplification of protein mixtures but is restricted by the accessibility of the often few ..
  98. ncbi The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana
    T P J Dunkley
    Department of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Building O, Downing Site, Cambridge CB2 1QW, UK
    Biochem Soc Trans 32:520-3. 2004
    ..For this reason, quantitative proteomics methods are being developed to enable the assignment of proteins to a specific component of the endomembrane system without the need to obtain pure organelles...
  99. ncbi Infrequent somatic mutations of the ICAT gene in various human cancers with frequent 1p-LOH and/or abnormal nuclear accumulation of beta-catenin
    Mitsuho Imai
    Department of Molecular Pathology, Tohoku University School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    Oncol Rep 12:1099-103. 2004
    ..b>ICAT, inhibitor of beta-catenin and TCF4/beta-catenin-interacting protein, was isolated and mapped to 1p36, a frequent ..
  100. ncbi A comparative proteomic strategy for subcellular proteome research: ICAT approach coupled with bioinformatics prediction to ascertain rat liver mitochondrial proteins and indication of mitochondrial localization for catalase
    Xiao Sheng Jiang
    Research Centre for Proteome Analysis, Key Lab of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China
    Mol Cell Proteomics 4:12-34. 2005
    ..To solve such a problem, we applied a high-throughput comparative proteome experimental strategy, ICAT approach performed with two-dimensional LC-MS/MS analysis, coupled with combinational usage of different ..
  101. ncbi Isotope-coded affinity tag (ICAT) approach to redox proteomics: identification and quantitation of oxidant-sensitive cysteine thiols in complex protein mixtures
    Mahadevan Sethuraman
    Vascular Biology Unit, Cardiovascular Proteomics Center, and Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA 02118, USA
    J Proteome Res 3:1228-33. 2004
    ..cysteine thiols in a complex protein mixture using a thiol-specific, acid-cleavable isotope-coded affinity tag (ICAT) reagent (Applied Biosystems, USA)...

Research Grants62

  1. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2013
    ..we will improve upon these methods by using proprietary photocleavable isotope coded affinity tagging reagents (PC-ICAT) for quantitative glycoproteomics to determine how O-GlcNAc patterns change, e.g. in normal and diseased states...
  2. Secretome Proteomics for VHL Tumor Biomarker Discovery
    Yuzuru Shiio; Fiscal Year: 2010
    ..Employing a quantitative proteomics technology, ICAT (isotope-coded affinity tag), a preliminary small- scale analysis of protein secretion from a VHL-null renal ..
  3. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2012
    ..These include isotope coded affinity tag (ICAT), multiplexed isobaric isotope reagent (iTRAQ) and metabolic labeling...
  4. FUNCTIONAL PROTEOMICS OF LEUKEMIC MARROW STROMAL CELLS
    Beerelli Seshi; Fiscal Year: 2011
    ..identify about 200 differentially expressed MPC proteins by an independent method, isotope-coded affinity tag (ICAT) labeling in conjunction with LC/MS/MS...
  5. High Performance Computational System to Support LCMS/Proteomics Analysis
    ROBERT STRAUBINGER; Fiscal Year: 2009
    ..workstations, depending on the matrix complexity and peptide modifications expected, the use of reagents such as ICAT or iTRAQ to quantify relative expression, and the proteome under study...
  6. Protein Biomarkers in Childhood Acute Myeloid Leukemia
    Antonio Bedalov; Fiscal Year: 2013
    ..Most currently available proteome profiling technologies (e.g. 2-D gels, ICAT, SILAC) have limitations that prevent their use for profiling large numbers of specimens...
  7. Potassium Channels as Macromolecular Complexes
    Thomas A Neubert; Fiscal Year: 2013
    ..Protein interactions will also be investigated using advanced proteomic approaches (ICAT &ITRAQ), which has the potential to uncover additional novel KATP channel interacting proteins...
  8. Functional Proteomics Study of Ephrin Signaling
    Thomas Neubert; Fiscal Year: 2003
    ..We propose to develop and use a new functional proteomics method based on isotope-coded affinity tag (ICAT) chemistry and liquid chromatography/mass spectrometry to identify proteins that participate in signal transduction ..
  9. 2D-HPLC-Tandem Mass Spectrometer for Proteomics
    Richard van Breemen; Fiscal Year: 2005
    ..used by many NIH-funded investigators for proteomic profiling using techniques such as isotope-coded affinity tags (ICAT)...
  10. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS J O'KEEFE; Fiscal Year: 2011
    ..b>ICAT is an intracellular protein that competitively binds ?-catenin and inhibits signaling...
  11. FUNCTIONAL PROTEOMICS OF LEUKEMIC MARROW STROMAL CELLS
    Beerelli Seshi; Fiscal Year: 2003
    ..identify about 200 differentially expressed MPC proteins by an independent method, isotope-coded affinity tag (ICAT) labeling in conjunction with LC/MS/MS...
  12. The Interactive Cholesterol Advisory Tool (ICAT)
    CHARLES SCOTT RIGBY; Fiscal Year: 2011
    ..with interactive technology to develop a highly-scalable solution-The Interactive Cholesterol Advisory Tool (ICAT)-designed to increase the initiation and maintenance of medication adherence and therapeutic lifestyle change in ..
  13. ProteomeX LC/MS System
    Michael Powell; Fiscal Year: 2004
    ..package for typical proteomics applications such as protein ID, multidimensional LC/MS, and protein profiling using ICAT reagents. The system builds on the very successful and popular LCQ Deca XP...
  14. Acquisition of a LC-MS/MS Proteomics System
    David Han; Fiscal Year: 2004
    ..Dr. David Han will utilize 3D-ICAT technology to identify and quantify apoptotic signaling proteins and engulfment ligands that are presented on ..
  15. Proteomics processing using networked instrument router*
    SANJAYA JOSHI; Fiscal Year: 2005
    ..The First Phase of study will use an LC-MS (Liquid Chromatography - Mass Spectroscopy) system that uses the ICAT (Isotope Coded Affinity Tags) technology developed at the lab of Dr...
  16. ApoE isoform-specific protein interactions in brain aging and neurodegeneration
    KATHLEEN MONTINE; Fiscal Year: 2007
    ..developed approach of high-throughput quantitative proteomic analysis combining isotope-coded affinity tagging (ICAT) with peptide analysis by LC/MS-MS allows us to identify and compare the relative abundance of apoE-associated ..
  17. Proteome-Wide Characterization of Phosphoproteins
    Richard Morrison; Fiscal Year: 2004
    ..The later phase of this project will develop methods that concomitantly combine PhIAT and ICAT labeling to identify proteins in glutamate or camptothecin treated p53+/+ cortical neurons that undergo changes in ..
  18. Novel markers for neonatal sepsis
    Ashok Reddy; Fiscal Year: 2005
    ..SELDI-TOF) mass spectrometry, (b) two-dimensional gel electrophoresis, and (c) isotope-coded affinity tagging (ICAT) to detect differentially expressed proteins...
  19. Role of COX-2 in UVB-induced beta-catenin signaling in keratinocytes
    Jill C Pelling; Fiscal Year: 2011
    ..Furthermore, we will employ a novel strategy for transdermal delivery of the [unreadable]-catenin inhibitor, ICAT, to test whether inhibition of [unreadable]-catenin signaling attenuates UVB-induced hyperplasia...
  20. Vasoregulation by IP3 receptor coupling to TRPC channels
    Adebowale Adebiyi; Fiscal Year: 2013
    ..release, and via IP3 receptor (IP3R)- and canonical transient receptor potential (TRPC) 3-dependent cation current (ICat) activation...
  21. Carol B Peterson; Fiscal Year: 2015
    ..The present study proposes to compare a new treatment for BED (Integrative Cognitive-Affective Therapy-BED;ICAT-BED) to an evidence based treatment for BED (Cognitive Behavior Therapy-Guided Self- Help;CBTgsh)...
  22. LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES
    LISA PFEFFERLE; Fiscal Year: 2005
    ..We shall start with improving the separation steps of a promising analytical method referred to as ICAT technology. Further we plan to enhance the speed of analysis of this approach by using elevated temperature...
  23. Molecular Definition of Mycobacterium paratuberculosis
    JULIA INAMINE; Fiscal Year: 2005
    ..This goal will be accomplished by four specific aims: 1) use standard proteomic methods and develop a new ICAT- based method to identify M...
  24. A Novel Implant-based Approach for Treating Retrognathic Hyperdivergent Patients
    Peter Buschang; Fiscal Year: 2007
    ..Tipping will be measured directly from dental models and 3D ICAT images...
  25. Proteomics Approaches to Interstitial Cystitis.
    Brian Liu; Fiscal Year: 2006
    ..levels of low- abundance urinary proteins in clinically annotated IC specimens with isotope-coded affinity tag (ICAT) and mass spectrometry; 4) mine proteomics data and to create predictive bioinformatics models (i.e...
  26. Mitochondrial Response to Oxidative Stress
    DANIEL BOGENHAGEN; Fiscal Year: 2006
    ..Both 2-D gel methods and quantitative isotope-coded affinity tag (ICAT) methods will be used to compare the abundance of mitochondrial proteins in control cells and cells exposed to ..
  27. Regulation of beta-Catenin Signaling by Tyrosine Phosphorylation
    Tong Chuan He; Fiscal Year: 2010
    ..second aim is to determine whether EGF regulates the interactions of P-catenin with its C-terminal binding partners ICAT and p300, by using immunoprecipitation and FRET protein interaction analysis...
  28. Mechanisms of prostate cancer prevention by lycopene
    Richard van Breemen; Fiscal Year: 2007
    ..lycopene and then investigate changes in protein expression both globally and on the sub-cellular level using the ICAT quantitative proteomics methodology...
  29. NEW METHODS FOR QUANTITATIVE GLYCOSPHINGOLIPIDOMICS
    STEVEN LEVERY; Fiscal Year: 2005
    ..In a later phase, synthesis of isotope coded affinity tags (sphingolipid ICAT, or SL-ICAT) is proposed, in order to facilitate quantitative glycosphingolipidomic profiling, analogous to the "..
  30. CHEMORECEPTION AND SIGNAL PROCESSING IN VAGAL AFFERENTS
    Daniel Weinreich; Fiscal Year: 2006
    ..and manipulated in order to clarify the signal pathway of NK-2 tachykinin receptor (NK2R)-mediated currents (Icat) unmasked by mast cell activation, or by serotonin...
  31. PSD protein expression in extinction of morphine-dependent conditioned behavior
    JOSE MORON CONCEPCION; Fiscal Year: 2007
    ..Relative levels of protein expression will be analyzed through isotope-coded affinity tag (ICAT) followed by tandem mass spectrometry (LC-MS/MS)...
  32. Diagnostic proteomic profiles for aneuploidy
    Ashok Reddy; Fiscal Year: 2004
    ..SELDI-TOF), 2-dimensional gel electrophoresis, and isotope-coded affinity tagging (ICAT) techniques to identify proteins and peptides that are differentially expressed in amniotic fluid and/or maternal ..
  33. UV-induced Immunosuppression to chemical toxicants
    Kevin Cooper; Fiscal Year: 2006
    ..broad identification of changes in the abundance of membrane-bound proteins using isotope coded affinity tagging (ICAT) and phosphoproteins using phosphoprotein isotope-coded affinity tags (PhlAT)...
  34. Finding membrane proteins in cutaneous T-cell lymphomas
    PRISKA VON HALLER; Fiscal Year: 2003
    ..the relative abundance of cell surface proteins between benign and malignant T-cells using the recently developed ICAT TM technology...
  35. A PROTEOMIC APPROACH TO THE INDENTIFICATION OF PROTEINS*
    Philip Mirkes; Fiscal Year: 2003
    ..by undertaking a quantitative analysis of complex embryo protein mixtures using isotope-coded affinity tags (ICAT)...
  36. Pathophysiology and Characterisation of NAFLD
    Michael Charlton; Fiscal Year: 2009
    ..logical extension of these experiments, we now propose to use high-throughput mass spectrometry analysis, using an ICAT tagging technique, to measure differential hepatic protein abundance, including of peroxisomal proteins, in ..
  37. MALDI-TOF MASS SPECTROMETER
    Bruce Ames; Fiscal Year: 2002
    ..with the capabilities to perform these tasks, as well as quantitative analysis using isotope coded affinity tags (ICAT), will greatly enhance our ability to simultaneously characterize multiple proteins potentially involved ..
  38. Integrative Cognitive-Affective Therapy for Bulimia Nervosa
    Stephen Wonderlich; Fiscal Year: 2009
    ..Integrative cognitive-affective therapy (ICAT) is a new, manual-based, psychological approach to the treatment of BN...
  39. PROTEOMIC MAPPING OF MYELIN AND ITS MEMBRANE SUBDOMAINS
    Rashmi Bansal; Fiscal Year: 2009
    ..changes in protein expression using Direct In-Gel Electrophoresis (DIGE) and Isotope-Coded Affinity Tag (ICAT) mass spectrometric methodologies, and manipulation of protein expression and function using antibody blocking, ..
  40. Aging of the subcellular muscle proteome
    Edgar Arriaga; Fiscal Year: 2005
    ..R21 application, our short-term goal is to use the concept of subcellular proteomes, carbonyl immuno-capture, and ICAT technology to identify (and discover) age-related carbonyl-associated proteins in muscle mitochondria...
  41. Quantifying Protein Predictors of Macrophage Responses
    Jared Roach; Fiscal Year: 2007
    ..To obtain the structure of these pathways we will use isotope-coded affinity tags (ICAT) to quantify early phosphorylation events triggered by TLR4 and TLR5, and correlate these events with specific ..
  42. Photocleavable ICAT Reagents for Quantitative Proteomics
    Jerzy Olejnik; Fiscal Year: 2004
    ..The recently introduced Istotope Coded Affinity Tags (ICAT) technique, which when used in conjunction with mass spectrometry allows changes in the abundance of specific ..
  43. Photocleavable ICAT Reagents for Quantitative Proteomics
    Mark Lim; Fiscal Year: 2007
    ..a new approach to quantitative proteomics based on the use of PhotoCleavable Isotope Coded Affinity Tags (PC-ICAT) along with mass spectrometry...
  44. QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETER - PROTEOMICS
    Carol Parker; Fiscal Year: 2002
    ..State-of-the-art proteomic methods couple 2D PAGE, or ICAT (isotope coded affinity tags) methods followed by capillary HPLC to mass spectrometry (MS) for quantitation and ..
  45. HUMAN PROTEIN DIFFERENTIAL DISPLAY ASSAY ICAT-ULC-MS/MS
    Babak Esmaeli Azad; Fiscal Year: 2001
    ..a protein differential display assay system, for proteomes of higher eukaryotes, using isotope-coded affinity tags (ICAT) and microcapillary liquid chromatography tandem mass spectrometry (uLC-MS/MS), is proposed...
  46. ACQUISITION OF A MASS SPECTROMETRY RESOURCE
    Leslie Goodwin; Fiscal Year: 2003
    ..has been designed and optimized for analysis of complex peptide and protein samples and is also well suited for ICAT (isotope-coded affinity tag) peptide quantitation...
  47. IDBEST Differential Display on Protein Chips
    LUKE SCHNEIDER; Fiscal Year: 2003
    ..Isotope tagging methods, such as ICAT, have the potential to significantly improve the precision of these methods, but cannot currently be applied to ..
  48. HAIR-BUNDLE PROTEOMICS
    Peter Gillespie; Fiscal Year: 2004
    ..In our first set of proposed experiments, we will use the isotope-coded affinity tag (ICAT) method to differentially label proteins expressed in hair bundles (comparing to hair- and supporting-cell somas, ..
  49. Melanoma Proteins in Serum and Urine
    LARRY TJOELKER; Fiscal Year: 2004
    ..proteins characteristic of different physiological conditions can be identified using isotope-coded affinity tags (ICAT) coupled with tandem mass spectrometry...
  50. B-CLL Proteins Quantified by Mass Spectrometry
    DAVID BARNIDGE; Fiscal Year: 2004
    ..As part of this proposal the candidate will take courses offered in the Mayo Graduate School on Techniques in Molecular Biology, Human Cytogenetics, and Immunology. ..
  51. Dietary Cysteine:Conceptal Response to Oxidative Stress
    Craig Harris; Fiscal Year: 2002
    ....
  52. Hindbrain Herniation in Sheep Model of Myelomeningocele
    Cornelia von Koch; Fiscal Year: 2002
    ..Furthermore, we will characterize in lambs gross pathological and axonal pathfinding anomalies usually seen in the human disease. ..
  53. TECHNOLOGY FOR AUTOMATED ANALYSIS OF PROTEOMES
    Steven Gygi; Fiscal Year: 2004
    ..Once developed such a technology will be an essential tool for biologists' attempts to interpret the linear information of genomes in terms of function, control and mechanisms of biological systems. ..
  54. Ecological Momentary Assessment of Anorexia Nervosa
    Stephen Wonderlich; Fiscal Year: 2009
    ..EMA methodology would be an excellent approach to understanding the relationship between environment and anorexic behavior, along with psychological mediators such as cognitive and emotional variables. ..
  55. THE ROLE OF ANNEXIN I DOWN-REGULATION IN PROSTATE CANCER
    David Ornstein; Fiscal Year: 2005
    ....
  56. Prostate Cancer Detection by Serum Proteomic Profiling
    David Ornstein; Fiscal Year: 2005
    ..5 to 10.0 ng/ml. Aim 4: Determine the biological variability of serum proteomic patterns by analyzing serum samples collected from the same patient on 3 consecutive days. ..
  57. The Role of C3a and C5a in BEA Induced Nephritis
    Michael Braun; Fiscal Year: 2006
    ..At the completion of this Award, the candidate will be prepared to continue as a highly productive independent investigator in the area of renal immunopathogenesis ..
  58. Emerging virulence factors in diarrheagenic E. coli pathogenesis
    PHILIP HARDWIDGE; Fiscal Year: 2006
    ..coli disease. [unreadable] [unreadable] [unreadable]..
  59. Urinary Biomarkers in Murine MPGN
    Michael Braun; Fiscal Year: 2006
    ....
  60. Proteomic Studies in Parkinson's Disease
    Jing Zhang; Fiscal Year: 2008
    ....
  61. Biomarkers for Parkinson's Disease (PD) and PD Dementia
    Jing Zhang; Fiscal Year: 2008
    ..abstract_text> ..