hSlu7

Summary

Gene Symbol: hSlu7
Description: SLU7 homolog, splicing factor
Alias: 9G8, hSlu7, pre-mRNA-splicing factor SLU7, SLU7 splicing factor homolog, splicing factor, step II splicing factor SLU7, zinc knuckle motif containing
Species: human

Top Publications

  1. ncbi Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
    J Y Chen
    Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
    Mol Cell 7:227-32. 2001
  2. ncbi The RNA splicing factor hSlu7 is required for correct 3' splice-site choice
    K Chua
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 402:207-10. 1999
  3. pmc PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels
    S Blanton
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington 40506 0225
    Mol Cell Biol 12:3939-47. 1992
  4. ncbi Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2
    T B Deb
    Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 271:2206-12. 1996
  5. ncbi Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5
    T Maeda
    Department of Radiology and Radiation Oncology, Osaka University School of Dentistry, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    Carcinogenesis 20:2341-4. 1999
  6. ncbi Age-dependent differential expression of fibronectin variants in skin and airway mucosal wounds
    Ha Sheng Li-Korotky
    Division of Pediatric Otolaryngology, Children s Hospital of Pittsburgh, 8100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Arch Otolaryngol Head Neck Surg 133:919-24. 2007
  7. pmc Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae
    C Geoffrey Burns
    Howard Hughes Medical Institute, Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Mol Cell Biol 22:801-15. 2002
  8. pmc Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production
    Sandrine Jacquenet
    Laboratoire de Médecine et Thérapeutique Moléculaire, INSERM CIC9501, 15 rue du Bois de la Champelle, 54500 Vandoeuvre les Nancy, France
    Retrovirology 2:33. 2005
  9. ncbi Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome
    A Wang
    Division of Respiratory Research, Hospital for Sick Children, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Hum Mol Genet 6:2117-26. 1997
  10. pmc Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing
    D Frank
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143
    Mol Cell Biol 12:5197-205. 1992

Research Grants

  1. SPLICING OF MRNA PRECURSORS
    Michael R Green; Fiscal Year: 2013
  2. Transcriptional elongation and splicing in human genes in situ
    Richard A Padgett; Fiscal Year: 2013
  3. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
  4. Phillip A Sharp; Fiscal Year: 2016
  5. The Role of RNA Splicing Factors in Retinal Degeneration
    Michael Farkas; Fiscal Year: 2012
  6. CATHERINE JU YING WU; Fiscal Year: 2016
  7. Red Cell Band 4.1 - Developmental Changes in RNA Splicing
    John G Conboy; Fiscal Year: 2010
  8. OLGA ANCZUKOW-CAMARDA; Fiscal Year: 2014
  9. REGULATION OF SPLICE SITE CHOICE IN C. ELEGANS
    ALAN ZAHLER; Fiscal Year: 2009
  10. Eric A Pierce; Fiscal Year: 2016

Detail Information

Publications248 found, 100 shown here

  1. ncbi Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
    J Y Chen
    Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
    Mol Cell 7:227-32. 2001
    ..Thus, in addition to unwinding RNA, the DExD/H box proteins may affect RNA-RNA rearrangements by antagonizing specific RNA-stabilizing proteins...
  2. ncbi The RNA splicing factor hSlu7 is required for correct 3' splice-site choice
    K Chua
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 402:207-10. 1999
    ..Here we describe a metazoan splicing factor (hSlu7) that is required for selection of the correct AG...
  3. pmc PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels
    S Blanton
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington 40506 0225
    Mol Cell Biol 12:3939-47. 1992
    An essential pre-mRNA splicing factor, the product of the PRP38 gene, has been genetically identified in a screen of temperature-sensitive mutants of Saccharomyces cerevisiae...
  4. ncbi Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2
    T B Deb
    Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India
    J Biol Chem 271:2206-12. 1996
    ..sequence has complete homology with the cDNA sequence of a protein P-32, co-purified with the human pre-mRNA splicing factor SF2 (Krainer, A.R., Mayeda, A., Kozak, D., and Binns, G. (1991) Cell 66, 383-394)...
  5. ncbi Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5
    T Maeda
    Department of Radiology and Radiation Oncology, Osaka University School of Dentistry, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    Carcinogenesis 20:2341-4. 1999
    ..Gene expression of three splicing regulatory factors, alternative splicing factor/splicing factor 2 (ASF/SF2), heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2) and the 65 kDa subunit of ..
  6. ncbi Age-dependent differential expression of fibronectin variants in skin and airway mucosal wounds
    Ha Sheng Li-Korotky
    Division of Pediatric Otolaryngology, Children s Hospital of Pittsburgh, 8100 Rangos Research Center, 3460 Fifth Avenue, Pittsburgh, PA 15213, USA
    Arch Otolaryngol Head Neck Surg 133:919-24. 2007
    ..From a rabbit model of airway mucosal wound healing, we isolated and cloned an RNA splicing factor, SRp20, that was coexpressed with Fn1 complementary DNA and suppressed in fetal wounds but induced in ..
  7. pmc Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae
    C Geoffrey Burns
    Howard Hughes Medical Institute, Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Mol Cell Biol 22:801-15. 2002
    ....
  8. pmc Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production
    Sandrine Jacquenet
    Laboratoire de Médecine et Thérapeutique Moléculaire, INSERM CIC9501, 15 rue du Bois de la Champelle, 54500 Vandoeuvre les Nancy, France
    Retrovirology 2:33. 2005
    ..we used a human cell line expressing high levels of complete HIV-1 and either one of the ASF/SF2, SC35, and 9G8 SR proteins...
  9. ncbi Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome
    A Wang
    Division of Respiratory Research, Hospital for Sick Children, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Hum Mol Genet 6:2117-26. 1997
    ..Hprp3p is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p...
  10. pmc Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing
    D Frank
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143
    Mol Cell Biol 12:5197-205. 1992
    ....
  11. pmc The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA
    S Teigelkamp
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 13:888-97. 1994
    The RNA helicase-like splicing factor PRP2 interacts only transiently with spliceosomes. To facilitate analysis of interactions of PRP2 with spliceosomal components, PRP2 protein was stalled in splicing complexes by two different methods...
  12. pmc The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins
    M Golovkin
    Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    Plant Cell 10:1637-48. 1998
    ..SRZ proteins are similar to human 9G8 splicing factor because they contain a zinc knuckle, precipitate with 65% ammonium sulfate, and cross-react with the 9G8 ..
  13. ncbi Identification of a pre-mRNA splicing factor, arginine/serine-rich 3 (Sfrs3), and its co-expression with fibronectin in fetal and postnatal rabbit airway mucosal and skin wounds
    Ha Sheng Li-Korotky
    Division of Pediatric Otolaryngology, Children s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA
    Biochim Biophys Acta 1762:34-45. 2006
    ..One encodes a rabbit fibronectin gene, Fn1, and another shares a high sequence homology to a human pre-mRNA splicing factor, arginine/serine-rich 3 (Sfrs3), coding for a RNA binding protein, SRp20...
  14. doi Poly(ADP-ribose) binds to the splicing factor ASF/SF2 and regulates its phosphorylation by DNA topoisomerase I
    Maria Malanga
    Department of Structural and Functional Biology, University Federico II, Via Cinthia, Monte S Angelo, Naples, Italy
    J Biol Chem 283:19991-8. 2008
    ..We found that, in the presence of both DNA and the alternative splicing factor/splicing factor 2 (ASF/SF2, a prototypical SR-protein), poly(ADP-ribose) affected topoisomerase I substrate ..
  15. pmc Evaluation of splicing efficiency in lymphoblastoid cell lines from patients with splicing-factor retinitis pigmentosa
    Lenka Ivings
    Section of Ophthalmology and Neuroscience, Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds, United Kingdom
    Mol Vis 14:2357-66. 2008
    ..The aim of this study was to use lymphoblast cell lines derived from RP patients to determine whether mutations in two of these splicing factors, PRPF8 and PRPF31, cause measurable deficiencies in pre-mRNA splicing...
  16. pmc RNA binding activity of heterodimeric splicing factor U2AF: at least one RS domain is required for high-affinity binding
    D Z Rudner
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3204, USA
    Mol Cell Biol 18:4004-11. 1998
    The pre-mRNA splicing factor U2AF (U2 small nuclear ribonucleoprotein particle [snRNP] auxiliary factor) plays a critical role in 3' splice site selection...
  17. pmc Functional and phenotypical comparison of myofibroblasts derived from biopsies and bronchoalveolar lavage in mild asthma and scleroderma
    Kristoffer Larsen
    Experimental Medical Science, Division of Vascular and Airway Research, Lund University, S 221 84 Lund, Sweden
    Respir Res 7:11. 2006
    ..Furthermore, we have compared phenotypic alterations in activated fibroblasts from BALF and bronchial biopsies from patients with mild asthma and SSc that may account for the distinct fibrotic responses...
  18. doi Subcutaneous fat shows higher thyroid hormone receptor-alpha1 gene expression than omental fat
    Francisco J Ortega
    Department of Diabetes, Endocrinology and Nutrition, Institut d Investigació Biomédica de Girona and CIBEROBN CB06 03 010, Instituto de Salud Carlos III, Girona, Spain
    Obesity (Silver Spring) 17:2134-41. 2009
    ..TR alpha, TR alpha 1, and TR alpha 2 mRNA levels, and the gene expressions of arginine-serine-rich, splicing factor 2 (SF2), heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1), heterogeneous nuclear ribonucleoprotein A1 (..
  19. pmc Vitamin A metabolite, all-trans-retinoic acid, mediates alternative splicing of protein kinase C deltaVIII (PKCdeltaVIII) isoform via splicing factor SC35
    Hercules Apostolatos
    James A Haley Veterans Hospital, Tampa, Florida 33612, USA
    J Biol Chem 285:25987-95. 2010
    ..Overexpression and knockdown of the splicing factor SC35 (i.e. SRp30b) indicated that it is involved in PKCdeltaVIII alternative splicing...
  20. ncbi Modulation of DNA topoisomerase I activity by p53
    C Gobert
    Rhone Poulenc Rorer SA, Centre de Recherche de Vitry Alfortville, Vitry sur Seine, France
    Biochemistry 35:5778-86. 1996
    ..topoisomerase I activity as measured by relaxation of supercoiled DNA and by phosphorylation of SR protein splicing factor. The increase in catalytic activity occurs in parallel with the nuclear accumulation of p53, resulting in ..
  21. ncbi Polypyrimidine tract-binding protein-associated splicing factor is a negative regulator of transcriptional activity of the porcine p450scc insulin-like growth factor response element
    R J Urban
    Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555 1060, USA
    Mol Endocrinol 14:774-82. 2000
    ..In this study, we showed that polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF), an RNA-binding component of spliceosomes, binds to the IGFRE...
  22. ncbi SAP155 Binds to ceramide-responsive RNA cis-element 1 and regulates the alternative 5' splice site selection of Bcl-x pre-mRNA
    Autumn Massiello
    Department of Biochemistry, Rm 2 016, Sanger Hall, Virginia Commonwealth University, 1101 East Marshall St, P O Box 980614, Richmond, Virginia 23298 0614, USA
    FASEB J 20:1680-2. 2006
    ..In this study, mass spectrometric analysis identified the splicing factor SAP155, as an RNA trans-acting factor binding to the purine-rich CRCE 1...
  23. pmc Assessment of SNPs associated with the human glucocorticoid receptor in primary open-angle glaucoma and steroid responders
    John H Fingert
    Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA
    Mol Vis 16:596-601. 2010
    ..Several arginine/serine-rich splicing factor (SR) proteins have been implicated in alternative splicing of the GR...
  24. pmc Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects
    D Z Rudner
    Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Proc Natl Acad Sci U S A 93:10333-7. 1996
    The essential eukaryotic pre-mRNA splicing factor U2AF (U2 small nuclear ribonucleoprotein auxiliary factor) is required to specify the 3' splice at an early step in spliceosome assembly...
  25. ncbi Association of the rat heterogeneous nuclear RNA-ribonucleoprotein F with TATA-binding protein
    T Yoshida
    Department of Biology, Faculty of Science, Chiba University, Chiba, Japan
    FEBS Lett 457:251-4. 1999
    Heterogeneous nuclear ribonucleoprotein F (hnRNP-F) has been shown to be a pre-mRNA splicing factor. Recent studies have uncovered the coordination of synthesis of pre-mRNA and its processing, including post-transcriptional modification ..
  26. pmc Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors
    Karen R Thickman
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    J Mol Biol 356:664-83. 2006
    ..complexes between the large subunit of the U2 small nuclear RNA auxiliary factor (U2AF65) with the splicing factor 1 (SF1) or the spliceosomal component SF3b155 are exchanged during a critical, ATP-dependent step of pre-mRNA ..
  27. pmc The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site
    X D Fu
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 89:1725-9. 1992
    The splicing factor SC35 is required for the first step of the splicing reaction and for the assembly of the earliest ATP-dependent complex detected by native gel electrophoresis (A complex)...
  28. pmc A conserved Drosophila transportin-serine/arginine-rich (SR) protein permits nuclear import of Drosophila SR protein splicing factors and their antagonist repressor splicing factor 1
    Eric Allemand
    Institut de Genetique Moleculaire, Unité Mixte Recherche 5535 du Centre National de la Recherche Scientifique, l Institut Fédératif de Recherches 24, F34293 Montpellier, France
    Mol Biol Cell 13:2436-47. 2002
    ..proteins prompted us to examine the mechanism by which Drosophila SR proteins and their antagonist repressor splicing factor 1 (RSF1) are imported into nucleus...
  29. pmc Novel splicing factor RBM25 modulates Bcl-x pre-mRNA 5' splice site selection
    Anyu Zhou
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Mol Cell Biol 28:5924-36. 2008
    ..These data suggest a novel mode for Bcl-x(S) 5' ss activation in which binding of RBM25 with exonic element CGGGCA may stabilize the pre-mRNA-U1 snRNP through interactions with hLuc7A...
  30. ncbi Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1
    An Boudrez
    Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B 3000 Leuven, Belgium
    J Biol Chem 277:31834-41. 2002
    ..expressed nuclear protein that functions both as a regulator of protein Ser/Thr phosphatase-1 and as a splicing factor. The N-terminal part of NIPP1 consists of a phosphothreonine-interacting Forkhead-associated (FHA) domain...
  31. pmc Alternative splicing regulation during C. elegans development: splicing factors as regulated targets
    Sergio Barberan-Soler
    Department of MCD Biology, University of California Santa Cruz, Santa Cruz, California, United States of America
    PLoS Genet 4:e1000001. 2008
    ..Among the genes with the most developmentally regulated alternatively splicing was the hnRNP F/H splicing factor homolog, W02D3.11 - now named hrpf-1...
  32. ncbi Relative amounts of antagonistic splicing factors, hnRNP A1 and ASF/SF2, change during neoplastic lung growth: implications for pre-mRNA processing
    Laura K Zerbe
    Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, Colorado 802262, USA
    Mol Carcinog 41:187-96. 2004
    ..Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and alternative splicing factor/splicing factor 2 (ASF/SF2) act antagonistically to modulate splice site selection...
  33. pmc The splicing factor Prp43p, a DEAH box ATPase, functions in ribosome biogenesis
    Nina B Leeds
    Department of Molecular Genetics and Cell Biology, University of Chicago, 920 E 58th St, Chicago, Illinois 60637, USA
    Mol Cell Biol 26:513-22. 2006
    ..efficient biogenesis of both small- and large-subunit rRNAs requires the DEAH box ATPase Prp43p, a pre-mRNA splicing factor. By steady-state analysis, a cold-sensitive prp43 mutant accumulates 35S pre-rRNA and depletes 20S, 27S, and ..
  34. doi Interplay between polypyrimidine tract binding protein-associated splicing factor and human myometrial progesterone receptors
    A J Tyson-Capper
    The Medical School, Institute of Cellular Medicine, Newcastle University, 3rd Floor, William Leech Building, Newcastle upon Tyne, UK
    J Mol Endocrinol 43:29-41. 2009
    ..that i) the human splicing and transcription factor, polypyrimidine tract binding protein-associated splicing factor (PSF), is spatially and temporally regulated in the myometrium during pregnancy and labour; ii) PSF ..
  35. ncbi Expression of Galectin-3 and Galectin-7 in thyroid malignancy as potential diagnostic indicators
    T H Than
    Department of Paraclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Lot 77 Sect 22, Jalan Tun Ahmad Zaidi Adruce, Kuching 93150, Malaysia
    Singapore Med J 49:333-8. 2008
    ..Galectins are beta-galactoside-binding proteins with Gal-3 being a redundant pre-mRNA splicing factor. They are supposed to be p53-related regulators in cell growth and apoptosis, being either anti-apoptotic or ..
  36. ncbi A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor
    D S Horowitz
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 11:139-51. 1997
    We have identified a human splicing factor required for the second step of pre-mRNA splicing. This new protein, hPrp18, is 30% identical to the yeast splicing factor Prp18...
  37. pmc Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43
    S Gee
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 94:11803-7. 1997
    Yeast splicing factor Prp43, a DEAH box protein of the putative RNA helicase/RNA-dependent NTPase family, is a splicing factor that functions late in the pre-mRNA splicing pathway to facilitate spliceosome disassembly...
  38. ncbi SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome
    J Rappsilber
    Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    J Biol Chem 276:31142-50. 2001
    ..The data indicate that SPF30 is an essential human splicing factor that may act to dock the U4/U5/U6 tri-snRNP to the A complex during spliceosome assembly or, alternatively, ..
  39. ncbi Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation
    K Harada
    Department of Immunology, Kurume University School of Medicine, Kurume, Fukuoka, Japan
    Int J Cancer 93:623-8. 2001
    ..a 2-hybrid screening in yeast for proteins interacting with SART3, and this method yielded a pre-mRNA splicing factor (RNA-binding protein prevalent during the S phase or RNA-binding protein with a serine-rich domain [RNPS1]) ..
  40. ncbi A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women and rhesus monkeys
    G Y Nie
    Prince Henry s Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia
    Reproduction 124:209-17. 2002
    b>Splicing factor SC35 is an essential component of the spliceosome, the cellular apparatus that removes introns from pre-mRNA to provide alternatively spliced isoforms...
  41. ncbi The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation
    Felicie F Andersen
    Department of Molecular and Structural Biology, University of Aarhus, C F Møllers Alle, Denmark
    J Mol Biol 322:677-86. 2002
    ..We demonstrate that the splicing factor ASF/SF2 inhibits relaxation by interfering with the DNA cleavage and/or DNA binding steps of human ..
  42. pmc CRS1, a chloroplast group II intron splicing factor, promotes intron folding through specific interactions with two intron domains
    Oren Ostersetzer
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Plant Cell 17:241-55. 2005
    ..We present a biochemical analysis of the interactions between a nuclear-encoded group II splicing factor and its chloroplast intron target...
  43. pmc The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site
    Katrin de Graaf
    Institute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany
    BMC Biochem 7:7. 2006
    ..DYRK1A is a nuclear protein kinase that has been localized to the splicing factor compartment...
  44. ncbi Survey of conserved alternative splicing events of mRNAs encoding SR proteins in land plants
    Kei Iida
    Faculty of Bio Science, Nagahama Institute of Bio Science and Technology, Siga, Japan
    Mol Biol Evol 23:1085-94. 2006
    ..These conserved AS events were found in the plant-novel-SR protein, SC35-like (SCL), and two-Zn-knuckles-type 9G8 subfamilies...
  45. ncbi Whitesnake/sfpq is required for cell survival and neuronal development in the zebrafish
    Laura Anne Lowery
    Whitehead Institute for Biomedical Research, and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Dev Dyn 236:1347-57. 2007
    ..We show that the whitesnake mutant corresponds to the sfpq (splicing factor, proline/glutamine rich) gene, encoding the PSF protein (polypyrimidine tract-binding protein-associated ..
  46. pmc Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex
    Juana Maria Gonzalez-Santos
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON, Canada
    Hum Mol Genet 17:225-39. 2008
    Mutations in PRPF3, a gene encoding the essential pre-mRNA splicing factor Hprp3p, have been identified in patients with autosomal dominant retinitis pigmentosa type 18 (RP18)...
  47. pmc Disease mechanism for retinitis pigmentosa (RP11) caused by missense mutations in the splicing factor gene PRPF31
    Susan E Wilkie
    University College London Institute of Ophthalmology, London, United Kingdom
    Mol Vis 14:683-90. 2008
    Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance...
  48. doi Decreased levels of the RNA splicing factor Prpf3 in mice and zebrafish do not cause photoreceptor degeneration
    John J Graziotto
    F M Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, Philadelphia, Pennsylvania, USA
    Invest Ophthalmol Vis Sci 49:3830-8. 2008
    ..Mutations in PRPF3 have been implicated in retinitis pigmentosa (RP) 18 through an unknown mechanism. The authors created and characterized Prpf3 knockout mice and zebrafish to determine whether RP18 is a result of haploinsufficiency...
  49. pmc Regulation of human neurotropic JC virus replication by alternative splicing factor SF2/ASF in glial cells
    Ilker Kudret Sariyer
    Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 6:e14630. 2011
    ....
  50. ncbi The Cdk9 and cyclin T subunits of TAK/P-TEFb localize to splicing factor-rich nuclear speckle regions
    C H Herrmann
    Dept of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    J Cell Sci 114:1491-503. 2001
    ..These results suggest that cyclin T1 functions to recruit its binding partners to nuclear speckles and raises the possibility that nuclear speckles are a site of TAK/P-TEFb function...
  51. ncbi A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice
    A Takahashi
    Department of Biochemistry, Department of Ophthalmology, Nagoya University School of Medicine, Tsurumai cho, Showa Ku, Nagoya 466 8550, Japan
    J Biochem 129:599-606. 2001
    Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68...
  52. ncbi Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40
    Silke Wiesner
    Structural Biology Programme, EMBL Heidelberg, Meyerhofstr 1, 69117 Heidelberg, Germany
    J Mol Biol 324:807-22. 2002
    The yeast splicing factor pre-mRNA processing protein 40 (Prp40) comprises two N-terminal WW domains, separated by a ten-residue linker, and six consecutive FF domains...
  53. ncbi Cloning of a novel RNA binding polypeptide (RA301) induced by hypoxia/reoxygenation
    N Matsuo
    Department of Anatomy and Neuroscience, Osaka University Medical School, Suita City, Japan
    J Biol Chem 270:28216-22. 1995
    ..showed two ribonucleoprotein domains and serine/arginine-rich domains, suggestive of their function as RNA splicing factor. Northern analysis displayed striking induction only in cultured astrocytes within 15 min of reoxygenation ..
  54. ncbi The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex
    H V Colot
    Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, Massachusetts 02254, USA
    Genes Dev 10:1699-708. 1996
    ..They contain pre-mRNA, U1 snRNP, and the splicing factor Mud2p and probably correspond to the mammalian E complexes, which contain pre-mRNA, U1 snRNP, and the ..
  55. ncbi Genetic depletion reveals an essential role for an SR protein splicing factor in vertebrate cells
    S M Mount
    Department of Microbiology, University of Maryland, College Park, USA
    Bioessays 19:189-92. 1997
    ..for genetic depletion of the chicken B cell line DT40 has revealed that the SR protein ASF/SF2 (alternative splicing factor/splicing factor 2) is essential for viability in these cells(1)...
  56. ncbi Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
    N Abovich
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 89:403-12. 1997
    ..Msl5p (renamed BBP for branchpoint bridging protein) has a mammalian ortholog, the splicing factor SF1...
  57. pmc A role for SRp54 during intron bridging of small introns with pyrimidine tracts upstream of the branch point
    C F Kennedy
    Verna and Marrs McLean Department of Biochemistry, Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 18:5425-34. 1998
    One of the earliest steps in pre-mRNA recognition involves binding of the splicing factor U2 snRNP auxiliary factor (U2AF or MUD2 in Saccharomyces cerevisiae) to the 3' splice site region...
  58. ncbi Identification of a regulated pathway for nuclear pre-mRNA turnover
    C Bousquet-Antonelli
    Wellcome Trust Centre for Cell Biology ICMB, University of Edinburgh, Scotland
    Cell 102:765-75. 2000
    ..When splicing was inhibited by mutation of a splicing factor, inhibition of turnover resulted in 20- to 50-fold accumulation of pre-mRNAs, accompanied by increased mRNA ..
  59. ncbi Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a c
    Yue Zhao
    Department of Medicine and Bioregulatory Science 3rd Department of Internal Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    J Biol Chem 277:30031-9. 2002
    ..to that of a protein that binds to U5 small nuclear ribonucleoprotein particle, a human homologue of yeast splicing factor Prp6p, involved in spliceosome...
  60. pmc p68 RNA helicase is an essential human splicing factor that acts at the U1 snRNA-5' splice site duplex
    Zhi Ren Liu
    Program in Cell and Molecular Biosciences, Department of Animal Sciences, Auburn University, 210 Upchurch Hall, Auburn, AL 36849, USA
    Mol Cell Biol 22:5443-50. 2002
    ..Furthermore, depletion of p68 RNA helicase arrested spliceosome assembly at the prespliceosome stage, suggesting that p68 may play a role in the transition from prespliceosome to spliceosome...
  61. pmc PSF and p54nrb bind a conserved stem in U5 snRNA
    Rui Peng
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA
    RNA 8:1334-47. 2002
    PTB-associated splicing factor (PSF) has been implicated in both early and late steps of pre-mRNA splicing, but its exact role in this process remains unclear...
  62. pmc Exonic splicing enhancer-dependent selection of the bovine papillomavirus type 1 nucleotide 3225 3' splice site can be rescued in a cell lacking splicing factor ASF/SF2 through activation of the phosphatidylinositol 3-kinase/Akt pathway
    Xuefeng Liu
    HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:2105-15. 2003
    ..Two exonic splicing enhancers, each containing two ASF/SF2 (alternative splicing factor/splicing factor 2) binding sites, are located between the two 3' splice sites and have been identified as ..
  63. pmc FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35
    Janet Chusainow
    Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    RNA 11:1201-14. 2005
    ..U2 snRNP Auxiliary Factor (U2AF) is an essential pre-mRNA splicing factor complex, comprising 35-kDa (U2AF35) and 65-kDa (U2AF65) subunits...
  64. ncbi Human splicing factor SPF45 (RBM17) confers broad multidrug resistance to anticancer drugs when overexpressed--a phenotype partially reversed by selective estrogen receptor modulators
    William L Perry
    Lilly Research Laboratories, Eli Lilly and Co, Indianapolis, Indiana 46285, USA
    Cancer Res 65:6593-600. 2005
    The splicing factor SPF45 (RBM17) is frequently overexpressed in many solid tumors, and stable expression in HeLa cells confers resistance to doxorubicin and vincristine...
  65. ncbi Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa
    Vladimir Pena
    AG Röntgenkristallographie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Mol Cell 25:615-24. 2007
    ..We conclude that the expanded Prp8 Jab1/MPN domain represents a pseudoenzyme converted into a protein-protein interaction platform and that dysfunction of this platform underlies Retinitis pigmentosa...
  66. pmc Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay
    Thomas Rio Frio
    Department of Medical Genetics, University of Lausanne, Lausanne, Switzerland
    J Clin Invest 118:1519-31. 2008
    Dominant mutations in the gene encoding the mRNA splicing factor PRPF31 cause retinitis pigmentosa, a hereditary form of retinal degeneration...
  67. doi The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors
    Natasa Skoko
    International Centre for Genetic Engineering and Biotechnology ICGEB, Padriciano 99, Trieste, Italy
    FEBS Lett 582:2231-6. 2008
    ..6792C>G mutation observing that this concurrently decreases the affinity for the positive splicing factor YB-1 and increases the affinity for the negative splicing factors, hnRNPA1, hnRNPA2 and a new player in these ..
  68. pmc Adenovirus serotype 5 L4-22K and L4-33K proteins have distinct functions in regulating late gene expression
    Susan J Morris
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom
    J Virol 83:3049-58. 2009
    ..MLTU expression is regulated at transcriptional and posttranscriptional levels. The L4-33K protein acts as a splicing factor to upregulate several MLTU splice acceptor sites as the late phase progresses...
  69. doi Plant-specific SR-related protein atSR45a interacts with spliceosomal proteins in plant nucleus
    Noriaki Tanabe
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, Japan
    Plant Mol Biol 70:241-52. 2009
    ..with themselves, other SR proteins (atSR45 and atSCL28), and PRP38-like protein, a homologue of the splicing factor essential for cleavage of the 5' splice site...
  70. doi Localization of poly(A)(+) RNA and mRNA export factors in interchromatin granule clusters of two-cell mouse embryos
    Irina Bogolyubova
    Laboratory of Cell Morphology, Institute of Cytology RAS, Tikhoretsky Avenue 4, 194064 St Petersburg, Russia
    Cell Tissue Res 338:271-81. 2009
    Interchromatin granule clusters (IGCs), also known as nuclear speckles, splicing factor compartments, or SC35-domains, are one of the most universal nuclear organelles of the cell...
  71. doi Monoclonal antibody that recognizes a domain on heterogeneous nuclear ribonucleoprotein K and PTB-associated splicing factor
    Gema Garcia-Jurado
    Departamento de Genetica, Universidad de Cordoba, Campus de Rabanales, Cordoba, Spain
    Hybridoma (Larchmt) 30:53-9. 2011
    ..Here we report the generation of one monoclonal antibody GG6H9.1C3 that recognized both hnRNP K and PSF proteins using Western blot analysis, flow cytometry, and immunocytochemistry...
  72. pmc Transcriptome instability in colorectal cancer identified by exon microarray analyses: Associations with splicing factor expression levels and patient survival
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, PO Box 4953 Nydalen, NO 0424 Oslo, Norway
    Genome Med 3:32. 2011
    ..In the present study we analyze genome-wide disruption of pre-mRNA splicing, and propose transcriptome instability as a characteristic that is analogous to genomic instability on the transcriptome level...
  73. doi TDP-43: new aspects of autoregulation mechanisms in RNA binding proteins and their connection with human disease
    Emanuele Buratti
    International Centre for Genetic Engineering and Biotechnology ICGEB, Trieste, Italy
    FEBS J 278:3530-8. 2011
    ..decay have also been described to play a role for other RNA binding protein factors: serine-arginine-rich splicing factor 1 (SRSF1) and transactive response DNA binding protein 43 kDa (TDP-43)...
  74. pmc PSF controls expression of histone variants and cellular viability in thymocytes
    Florian Heyd
    Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, 422 Curie Blvd, Philadelphia, PA 19104 6059, USA
    Biochem Biophys Res Commun 414:743-9. 2011
    ..to investigate the function of the essential protein PSF (polypyrimidine tract binding protein-associated splicing factor) in thymocytes...
  75. pmc WT1 mutants reveal SRPK1 to be a downstream angiogenesis target by altering VEGF splicing
    Elianna M Amin
    Centre for Research in Biomedicine, University of the West of England, Bristol, UK
    Cancer Cell 20:768-80. 2011
    ..Altered VEGF splicing was reversed by wild-type WT1, knockdown of SRSF1, or SRPK1 and inhibition of SRPK1, which prevented in vitro and in vivo angiogenesis and associated tumor growth...
  76. doi Annexin A2 and PSF proteins interact with p53 IRES and regulate translation of p53 mRNA
    Arandkar Sharathchandra
    Department of Microbiology and Cell Biology Indian Institute of Science Bangalore, India
    RNA Biol 9:1429-39. 2012
    ..In the current study, using RNA affinity approach we have identified Annexin A2 and PTB associated Splicing Factor (PSF/SFPQ) as novel ITAFs for p53 IRESs...
  77. pmc U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs
    Jermaine L Jenkins
    Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Nucleic Acids Res 41:3859-73. 2013
    ..The essential pre-mRNA splicing factor U2AF(65) is faced with the paradoxical tasks of accurately targeting polypyrimidine (Py) tracts preceding 3' ..
  78. pmc Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly
    Shataparna Banerjee
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
    Mol Cell Biol 33:3125-36. 2013
    ..The data imply dynamic substrate-splicing factor relationships in multiintron transcripts...
  79. doi Phosphorylation drives a dynamic switch in serine/arginine-rich proteins
    Shengqi Xiang
    Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen 37077, Germany
    Structure 21:2162-74. 2013
    ..Here, we show that phosphorylation switches the RS domain of the serine/arginine-rich splicing factor 1 from a fully disordered state to a partially rigidified arch-like structure...
  80. doi Coupling between transcription and alternative splicing
    Ignacio E Schor
    Laboratorio de Fisiologia y Biologia Molecular, Departmento de Fisiologia, Biologia Molecular y Celular, IFIBYNE CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pab II, 20 Piso, Buenos Aires, 1428, Argentina
    Cancer Treat Res 158:1-24. 2013
    ..efficiency and kinetics by directing the nascent pre-mRNA into proper spliceosome assembly and favoring splicing factor recruitment...
  81. pmc Emerging functions of SRSF1, splicing factor and oncoprotein, in RNA metabolism and cancer
    Shipra Das
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
    Mol Cancer Res 12:1195-204. 2014
    Serine/Arginine Splicing Factor 1 (SRSF1) is the archetype member of the SR protein family of splicing regulators...
  82. pmc Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway
    Bahityar Rahmutulla
    Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba City, Chiba, Japan
    Oncotarget 5:2404-17. 2014
    ..FIR and FIRΔexon2 form homo- or heterodimers that complex with SAP155. SAP155, a subunit of the essential splicing factor 3b subcomplex in the spliceosome, is required for proper P27Kip1 pre-mRNA splicing, and P27Kip1 arrests cells ..
  83. pmc Regulation of human adenovirus alternative RNA splicing by the adenoviral L4-33K and L4-22K proteins
    Roberta Biasiotto
    Department of Medical Biochemistry and Microbiology, Science for Life Laboratory Uppsala University, Uppsala 75123, Sweden
    Int J Mol Sci 16:2893-912. 2015
    ..L4-33K is a viral alternative RNA splicing factor that controls L1 alternative splicing via an interaction with the cellular protein kinases Protein Kinase A (..
  84. doi Upregulation of SYF2 Relates to Retinal Ganglion Cell Apoptosis and Retinal Glia Cell Proliferation After Light-Induced Retinal Damage
    Aimin Sang
    Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, China
    J Mol Neurosci 56:480-90. 2015
    SYF2 (SYF2 homologue, RNA splicing factor), also known as CCNDBP1-interactor or p29, belongs to the SYF2 family, which are involved in pre-mRNA splicing and cell cycle progression...
  85. pmc A germline mutation in SRRM2, a splicing factor gene, is implicated in papillary thyroid carcinoma predisposition
    Jerneja Tomsic
    Department of Molecular Virology, Immunology and Medical Genetics, Ohio State University Wexner Medical Center and Comprehensive Cancer Center, Ohio State University, Columbus, Ohio, United States of America
    Sci Rep 5:10566. 2015
    ..The results confirmed a higher ratio of inclusion of exons in mutation carriers. These data suggest that the S346F mutation in SRRM2 predisposes to PTC by affecting alternative splicing of unidentified downstream target genes...
  86. doi Genome-wide lentiviral shRNA screen identifies serine/arginine-rich splicing factor 2 as a determinant of oncolytic virus activity in breast cancer cells
    S T Workenhe
    Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, Institute for Infectious Disease Research, McMaster University, Hamilton, ON, Canada
    Oncogene 35:2465-74. 2016
    ..oncolysis, this study used a pooled genome-wide short hairpin RNA library and found that depletion of the splicing factor arginine-rich splicing factor 2 (SRSF2) leads to enhanced cytotoxicity of breast cancer cells by KM100...
  87. pmc Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family
    Y Cavaloc
    Unite 184 de Biologie Moleculaire et de Genie Genetique de l INSERM, Faculte de Medecine, Strasbourg, France
    EMBO J 13:2639-49. 1994
    By adopting a monoclonal antibody approach, we have identified a novel splicing factor of 35 kDa which we have termed 9G8...
  88. pmc A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site
    O Gozani
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 18:4752-60. 1998
    ..The splicing factor U2AF binds to the pyrimidine tract at the 3' splice site in the earliest spliceosomal complex, E, and is ..
  89. pmc Assembly of smooth muscle myosin by the 38k protein, a homologue of a subunit of pre-mRNA splicing factor-2
    T Okagaki
    Department of Pharmacology, Gunma University School of Medicine, Maebashi, Gunma 371 8511, Japan
    J Cell Biol 148:653-63. 2000
    ..not homologous to telokin, but to human p32, which was originally found in nuclei as a subunit of pre-mRNA splicing factor-2. Western blotting showed that the protein was expressed in various smooth muscles...
  90. ncbi Computational analysis of alternative splicing using EST tissue information
    Hanqing Xie
    Compugen Inc 7 Centre Drive, Jamesburg, New Jersey 08831, USA
    Genomics 80:326-30. 2002
    ..Several genes, including one encoding a possible pre-mRNA splicing factor, displayed cancer-specific alternative splicing...
  91. ncbi Molecular architecture of the multiprotein splicing factor SF3b
    Monika M Golas
    Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Science 300:980-4. 2003
    The splicing factor SF3b is a multiprotein complex essential for the accurate excision of introns from pre-messenger RNA...
  92. ncbi The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis
    Jin Xu
    Department of Neurology, Caritas St Elizabeth s Center, Tufts University School of Medicine, Boston, MA 02135, USA
    Hum Mol Genet 14:1231-41. 2005
    ..dopaminergic neuronal cells are the nuclear proteins p54nrb and pyrimidine tract-binding protein-associated splicing factor (PSF), two multifunctional regulators of transcription and RNA metabolism...
  93. ncbi Mass spectroscopy identifies the splicing-associated proteins, PSF, hnRNP H3, hnRNP A2/B1, and TLS/FUS as interacting partners of the ZNF198 protein associated with rearrangement in myeloproliferative disease
    Chitta S Kasyapa
    Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Exp Cell Res 309:78-85. 2005
    ..P splicing factor (PSF) was identified as one of the proteins and this interaction was confirmed by Western blotting...
  94. pmc Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing
    Junlin Qi
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center and Genes and Development Graduate Program, The University of Texas Graduate School of Biomedical Sciences, Houston, TX 77030 4009, USA
    Nucleic Acids Res 34:6256-63. 2006
    The splicing factor Transformer-2 (Tra2) is expressed almost ubiquitously in Drosophila adults, but participates in the tissue-specific regulation of splicing in several RNAs...
  95. pmc Subcellular transport of EKLF and switch-on of murine adult beta maj globin gene transcription
    Yu Chiau Shyu
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China
    Mol Cell Biol 27:2309-23. 2007
    ..is colocalized the PML oncogene domain nuclear body, and concentrated with EKLF, RNA polymerase II, and the splicing factor SC35...
  96. pmc The maize zmsmu2 gene encodes a putative RNA-splicing factor that affects protein synthesis and RNA processing during endosperm development
    Taijoon Chung
    Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, USA
    Plant Physiol 144:821-35. 2007
    ....
  97. pmc Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways
    Jérôme Barbier
    INSERM U685, Equipe Avenir, Hopital Saint Louis, 1 avenue Claude Vellefaux, Paris 75010, France
    Mol Cell Biol 27:7315-33. 2007
    ..camptothecin effects on H-ras gene expression were p53 dependent and involved in part modulation of the SC35 splicing factor. Interestingly, a long-term treatment with camptothecin as well as p53 overexpression for 24 h resulted in ..
  98. ncbi The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha
    Maria Buxade
    Division of Molecular Physiology, College of Life Sciences, University of Dundee, Dundee, UK
    J Biol Chem 283:57-65. 2008
    ..in vitro, and identified one such protein as PSF (the PTB (polypyrimidine tract-binding protein)-associated splicing factor). Mnks phosphorylate PSF at two sites in vitro, and our data show that PSF is an Mnk substrate in vivo...
  99. pmc Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway
    Isabelle Ote
    Laboratory of Virology and Immunology, GIGA R, University of Liege ULg, Liege, Belgium
    PLoS ONE 4:e7882. 2009
    ..and immunoprecipitation analysis, we showed that IE4 interacts with three shuttling SR proteins, namely ASF/SF2, 9G8 and SRp20...
  100. doi The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression
    Teresa Ezponda
    Department of Pathology, Clinica Universidad de Navarra, Pamplona, Spain
    Clin Cancer Res 16:4113-25. 2010
    SF2/ASF is a splicing factor recently described as an oncoprotein...
  101. doi Stress induced subcellular distribution of ALG-2, RBM22 and hSlu7
    Aleksandra Janowicz
    Department of Biochemistry, School of Molecular and Systems Biology, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 1813:1045-9. 2011
    ..b>hSlu7, another spliceosomal nuclear protein, known to interact with RBM22 in yeast, has been shown to translocate to the ..

Research Grants63

  1. SPLICING OF MRNA PRECURSORS
    Michael R Green; Fiscal Year: 2013
    ..The U2 snRNP Auxiliary Factor (U2AF) is an essential splicing factor, originally identified in our laboratory, that binds to the polypyrimidine (Py)-tract/3'splice site and ..
  2. Transcriptional elongation and splicing in human genes in situ
    Richard A Padgett; Fiscal Year: 2013
    ..We propose to use a variation of our previous experiment to investigate the temporal order of splicing factor binding to long introns in order to test current theories of exon definition...
  3. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
    ..For example, the gene encoding the splicing factor SF2/ASF is a proto-oncogene that can be activated by gene amplification in breast cancer...
  4. Phillip A Sharp; Fiscal Year: 2016
    ..ANG1), important in angiogenesis and structurally a member of the ribonuclease family, and SRp54, an RNA splicing factor known to be important for inclusion of exon 10 in the tau protein...
  5. The Role of RNA Splicing Factors in Retinal Degeneration
    Michael Farkas; Fiscal Year: 2012
    ..This data is consistent with results from studies of another form of disease caused by mutations in an RNA splicing factor. In this case, the neurologic disorder spinal muscular atrophy (SMA), which is caused by mutations in the ..
  6. CATHERINE JU YING WU; Fiscal Year: 2016
    ....
  7. Red Cell Band 4.1 - Developmental Changes in RNA Splicing
    John G Conboy; Fiscal Year: 2010
    ..pre-mRNA splicing in differentiating erythroid cells, focusing on cis-regulatory sequences and trans-acting splicing factor proteins that regulate erythroid stage-specific switches in exon splicing...
  8. OLGA ANCZUKOW-CAMARDA; Fiscal Year: 2014
    ..We have previously demonstrated that overexpression of the splicing factor SRSF1 can transform human mammary epithelial cells in vitro and in vivo...
  9. REGULATION OF SPLICE SITE CHOICE IN C. ELEGANS
    ALAN ZAHLER; Fiscal Year: 2009
    ..2- To determine the mechanism of action of the pre-mRNA splicing factor MEC-8 in regulation of pre-mRNA splicing...
  10. Eric A Pierce; Fiscal Year: 2016
    ..long-term objectives of the proposed research are to improve our understanding of the pathogenesis of the RNA splicing factor forms of retinitis pigmentosa (RP) so that therapies for these blinding disorders can be developed...
  11. Exon recognition during constitutive pre-mRNA splicing
    Lawrence A Chasin; Fiscal Year: 2013
    ..After establishment of the methodology using one model RBP (the pre- mRNA splicing factor Fox-2), the method will be extended to additional related splicing factors...
  12. The Identification and Manipulation of RNA Splicing Factor Regulators in Spinal M
    MALLORY ANN HAVENS; Fiscal Year: 2013
    ..abstract_text> ..
  13. Marisa Wong Medina; Fiscal Year: 2014
    ..These findings lead to the hypotheses that intracellular cholesterol levels regulate splicing factor(s) to generate coordinated changes in alternative splicing of multiple genes involved in cholesterol ..
  14. ASF/SF2: Phosphorylation and RNA Binding
    Gourisankar Ghosh; Fiscal Year: 2012
    Alternating splicing factor/splicing factor 2 (ASF/SF2), a member of the serine-arginine (SR) protein family, plays important roles both in constitutive and alternative pre-mRNA splicing...
  15. Understanding the structure and function of splicing factor Prp8
    Rui Zhao; Fiscal Year: 2012
    ..Mutations in splicing factor Prp8, for example, cause a severe form of human genetic disorder Retinitis Pigmentosa...
  16. LIOR S PACHTER; Fiscal Year: 2015
    ..in Aims 1 and 2 by using computational and biochemical approaches to analyze cis- regulatory sequences and splicing factor proteins that direct these splicing networks...
  17. James L Manley; Fiscal Year: 2015
    ..Protein purification and MS have identified the evolutionarily conserved splicing factor Prp5 as a strong candidate...
  18. Intravenous Protein Therapy for Myotonic Dystrophy Type 1
    Richard Weisbart; Fiscal Year: 2010
    ..within the transcribed DMPK mRNA avidly binds, sequesters and inactivates the MBNL1 protein, an mRNA splicing factor that removes fetal exons from mRNA templates (9- 13)...
  19. Regulation of Ovarian Cancer Multidrug Resistance by MAP Kinases
    Scott T Eblen; Fiscal Year: 2013
    ..Alternative mRNA splicing affects 74% of the human genome. Alterations in alternative mRNA splicing factor expression occur in cancer and can strongly influence apoptotic responses...
  20. Xiang Dong Fu; Fiscal Year: 2016
    ..The proposed research along this line has clear disease relevance. ..
  21. The Role of Alternative Splicing Factor Sfrs10 in Neural Development
    Rahul N Kanadia; Fiscal Year: 2010
    ....
  22. Global analysis of hnRNPLL-mediated alternative pre-mRNA splicing in lymphocytes.
    Shalini Oberdoerffer; Fiscal Year: 2009
    ..L-like (hnRNPLL) as an inducible, critical regulator of activation- induced CD45 alternative splicing in a splicing factor RNA interference (RNAi) screen...
  23. STORMY JO CHAMBERLAIN; Fiscal Year: 2015
    ..SNURF-SNRPN, including some of the downstream non-coding exons, as a target for FOX2, a tissue specific splicing factor in human embryonic stem cells (hESCs)...
  24. Programmed Changes in Alternative Splicing Within the Erythroid Transcriptome
    JOHN CONBOY; Fiscal Year: 2007
    ..alternative splicing program in human and mouse models, and (2) Functionally assess the role of selected splicing factor proteins in erythroid differentiation by measuring splicing changes that occur in response to RNAi-mediated ..
  25. Deep Sequencing Analysis of mRNA Isoform Expression Changes in Myotonic Dystrophy
    David Housman; Fiscal Year: 2010
    ..gene fold into hairpins that accumulate in nuclear foci, resulting in effective depletion of the alternative splicing factor Muscleblind (MBNL1) and hyperactivation of the splicing factor CUG Binding Protein 1 (CUGBP1)...
  26. FUNCTION OF MAMMALIAN PREMRNA SPLICING FACTORS
    Jane Wu; Fiscal Year: 2000
    ..factors, one identified by its structural characteristics and two isolated by their interactions with a known splicing factor. Experiments are also proposed to investigate the possible roles of the RS domain containing proteins in ..
  27. FUNCTION OF SNRNP PARTICLES IN PRE-MRNA SPLICING
    Brian Rymond; Fiscal Year: 2007
    ..First, that a spliceosome cycle occurs within cells and progresses through a series of splicing factor-dependent assembly and disassembly steps...
  28. The Role of Alternative Splicing Factor Sfrs10 in Neural Development
    Rahul N Kanadia; Fiscal Year: 2013
    ..Extenstion of this work in my laboratory will provide me with preliminary data that 1 intend to use for an ROl grant that should launch my independent career in science. ..
  29. Ilker Kudret Sariyer; Fiscal Year: 2016
    ..We recently identified the alternative splicing factor, SF2/ASF, as a potential regulator of JCV as its overexpression in glial cells strongly suppresses viral gene ..
  30. VAISHALI MOULTON; Fiscal Year: 2016
    ..Among these proteins, a promising candidate is the serine arginine (SR) family member Alternative Splicing Factor/Splicing Factor 2 (ASF/SF2)...
  31. Melissa J Moore; Fiscal Year: 2016
    ..Single Molecule Spectroscopy (CoSMoS) approach, one can directly observe the detailed kinetics of snRNP and splicing factor association/dissociation with pre-mRNA over the full course of spliceosome assembly and splicing...
  32. Houng Wei Tsai; Fiscal Year: 2015
    ..Recently, we have identified splicing factor, suppressor of white-apricot homolog (Drosophila) (Sfswap), also known as splicing factor arginine/serine-..
  33. WOODRING ERIK WRIGHT; Fiscal Year: 2015
    ..to reduce the amount of functional protein produced will be used: 1) Oligonucleotides directed against splicing factor binding sites have been shown to be able to cause exon-skipping, and are in clinical trials for a number of ..
  34. Maria L Spletter; Fiscal Year: 2014
    ..I propose to investigate the splicing factor Arrest to gain mechanistic insight into how Salm induced alternative splicing may dictate the development of ..
  35. Functional Analysis of CDKp110 Protein Kinase
    Jill M Lahti; Fiscal Year: 2010
    ..This function is unique among the CDKs, with the RS-rich general pre-mRNA splicing factors RNPS1 and 9G8 identified as bona fide interactors. CDK11p110/p58-null mouse embryos fail to develop past E3...
  36. Splicing factors as therapeutic targets for the treatment of ovarian cancer
    William T Beck; Fiscal Year: 2012
    ..In this proposal, we focus on one splicing factor, PTB, and one tumor type, EOC, and hypothesize that PTB is a druggable therapeutic target in this disease...
  37. Samuel C Dudley; Fiscal Year: 2016
    ..angiotensin II (AngII) can signal pathological SCN5a splicing regulation by inducing expression of the mRNA splicing factor, CROP/hLuc7A, and its co-factor RBM25, which alter SCN5a splicing regulation by interacting with one or more ..
  38. CARBOHYDRATE BINDING PROTEIN 35
    John Wang; Fiscal Year: 2006
    ..of pre-mRNA in a cell-free assay, depletion and reconstitution experiments demonstrated that galectin-3 was a splicing factor. In the course of these studies, we found another member of the galectin family, galectin-1 (Mr -14,000), was ..
  39. STACEY DENISE WAGNER; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): Muscleblind-like 1 (MBNL1) is an alternative splicing factor whose improper sequestration is a primary component of the genetic disease myotonic dystrophy (DM)...
  40. Examining role of splicing factor mutations in myelodysplastic syndrome (PQ11)
    Shalini Sharma; Fiscal Year: 2013
    ..The goal of this project is to determine the molecular effects of MDS mutations on splicing factor function and to identify genome-wide splicing aberrations they induce during hematopoiesis that contribute to ..
  41. RNA/PROTEIN INTERACTIONS IN PREMRNA SPLICING REGULATION
    Ravinder Singh; Fiscal Year: 2003
    ..In the past, we defined the mechanism by which SXL regulates alternative splicing by antagonizing the known splicing factor U2AF65. Specificity is an underlying theme in biological regulation...
  42. GENERAL PRE-MRNA SPLICING FACTORS IN DROSOPHILA
    Donald Rio; Fiscal Year: 2001
    ..the mechanisms involved in the function of the conserved arginine-serine (R/S) domain containing pre-mRNA splicing factor U2 snRNP auxiliary factor (U2AF) in the fruit fly, Drosophila melanogaster...
  43. JAMES T BRENNA; Fiscal Year: 2016
    ..A newly described FADS1 AT has desaturase function and new siRNA data show that a specific splicing factor (SF), PTB (hnRNP I) modulates FADS AT abundance...
  44. PROCESSING OF PRECURSORS TO MRNA IN DROSPHILA
    STEPHEN MOUNT; Fiscal Year: 1999
    ..Additional tests of the dominant B52 allele will involve testing its ability to function as a basal splicing factor in complementation of S100 extracts from Drosophila or Hela cells and tests of its ability to alter 5' splice ..
  45. GENETICS OF YEAST PREMRNA SPLICING
    John Woolford; Fiscal Year: 1999
    ..Spp2p is the first protein identified that may interact with a DEAD(H) box splicing factor. Studies of Spp2p are designed to help understand how Prp2p and Spp2p assemble into the spliceosome and ..
  46. Dong Er Zhang; Fiscal Year: 2016
    Project Summary/Abstract Recent large-scale sequencing efforts have revealed prevalent mutations in splicing factor-encoded genes in bone marrow failure diseases, such as myelodysplastic syndromes (MDS) and related disorders...
  47. THYROID/RETINOIC ACID RECEPTOR INTERACTING PROTEINS
    Mukul Mathur; Fiscal Year: 2004
    ..The 105 kDa protein is a splicing factor known as PSF (PTB associated splicing factor)...
  48. HUMAN CYTOMEGALOVIRUS TEGUMENT PROTEINS
    Thomas E Shenk; Fiscal Year: 2013
    ..in epithelial cells, and we have shown that it binds to the cellular SF2/ASF protein, a multi-functional splicing factor. We will investigate the host range function of pUS22 in epithelial cells, and we will explore the ..
  49. MARK ANTHONY CLEMENTZ; Fiscal Year: 2014
    ..hypothesis is that N86-hnRNPU affects cellular factors involved in mRNA export such as TAP and the SR protein 9G8. The later has been shown to act as an export adaptor for TAP-mediated export and bind to HIV-1 mRNAs...
  50. Mechanism of SR Protein Binding to the Splicing Kinase SRPK1
    RYAN MATTHEW PLOCINIK; Fiscal Year: 2011
    ..goal is to understand the SRPK1-ASF/SF2 assembly mechanism and to establish fundamental principles for splicing factor recognition and biological control within the larger SR protein family...
  51. Pre-mRNA Splicing in S. pombe: Genetics and Genomics
    JO WISE; Fiscal Year: 2004
    ..cell extracts have led to a model in which enhancer-bound SR proteins contact the heterodimeric general splicing factor U2AF and stabilize its association with weak 3' splice sites...
  52. The Fate of dsRNA in the Nucleus
    Gordon Carmichael; Fiscal Year: 2006
    ..This complex contains the inosine-specific RNA binding protein p54nrb, the splicing factor PSF and the inner nuclear matrix structural protein matrin 3...
  53. U2AF AND SPLICE SITE RECOGNITION IN C ELEGANS
    Thomas Blumenthal; Fiscal Year: 2003
    ..The genes, uaf-1 and uaf-2, encode large and small subunits of the essential splicing factor U2AF, which are responsible for recognition of this 3' splice site consensus...
  54. Investigating mechanisms of skeletal muscle degeneration in Myotonic Dystrophy
    James P Orengo; Fiscal Year: 2010
    ..In Specific Aim 3 a splicing factor, CUGBP1, which regulates developmental alternative splicing patterns in post-natal development, will be ..
  55. Regulation of Polyadenylation: the U1A/U1snRNP Paradigm
    Samuel Gunderson; Fiscal Year: 2007
    ..the first funding period of this grant we: 1) characterized a unique form of polyA tail regulation by the U1A splicing factor, 2) discovered that U1A, via binding non-consensus pre-mRNA sequences, can regulate polyA tail addition to ..
  56. GENE EXPRESSION IN HUMAN PARASITIC NEMATODES
    TIMOTHY NILSEN; Fiscal Year: 2009
    ..Second, the evidence indicates that SL-30 makes essential contacts with the splicing factor SF1/BBP, a protein that plays a bridging role between 5'and 3'splice sites in cis-splicing...
  57. STAT FACTOR AND SIGNAL TRANSDUCTION AND CANCER
    Xin Yuan Fu; Fiscal Year: 2000
    ..James Manley's laboratory at Columbia University, where he was the first to identify the alternative splicing factor, ASF. When he was a postdoctoral fellow with Dr...
  58. Splice site recognition and protein assembly
    GWENDOLYN JANG; Fiscal Year: 2009
    ..Subsequent experiments will investigate the effects of SR/hnRNP proteins on splicing factor-splice site interactions using established regulatory elements and artificial recruitment strategies in a ..
  59. Structural Analyses of Fission Yeast Splicing Complexes
    KATHLEEN GOULD; Fiscal Year: 2006
    ..By targeting the splicing factor Cdc5p for tandem affinity purification, we have purified a stable 37S spliceosomal-like particle from the ..
  60. BIAcore 3000 System
    LESLIE GRIFFITH; Fiscal Year: 2002
    ..interactions of IMPPDH; regulation of exonuclease ssDNA binding by auxiliary subunits; characterization of splicing factor protein-protein interactions; measurement of Ab:Ag affinities during B cell differentiation; identification ..
  61. RNA DYNAMICS IN THE SPLICEOSOME
    DAVID BROW; Fiscal Year: 2006
    ..e., RNA chaperones). Aim 2 will focus on the function of splicing factor Prp24, a prime candidate for a U6 RNA chaperone, in unwinding of the U4/U6 complex during spliceosome ..
  62. SPLICING ENHANCER REGULATION OF GROWTH HORMONE ISOFORMS
    Fritz Rottman; Fiscal Year: 1999
    ..2. The mechanism whereby the exon specific enhancer (ESE)-splicing factor complex proceeds to activate suboptimal splice sites will be defined by determining the additional splicing ..
  63. MOLECULAR MECHANISMS WHEREBY CTG EXPANSION RESULTS IN DM
    Sita Reddy; Fiscal Year: 2004
    ..breaks in the CTG repeat tracts or pre-mRNA splicing abnormalities resulting from the titration of the splicing factor CUG-BP by the expanded CUG repeats are the molecular mechanism whereby expanded CTG/CUG repeats result in DM ..