histone H3

Summary

Gene Symbol: histone H3
Description: H3 histone family member 3A
Alias: H3.3A, H3F3, histone H3.3, H3 histone, family 3A
Species: human

Top Publications

  1. doi HIV-1 Tat protein induces IL-10 production in monocytes by classical and alternative NF-kappaB pathways
    Kaoutar Leghmari
    Laboratoire d Immuno Virologie, EA 3038, Universite Paul Sabatier, 118, route de Narbonne, Bâtiment 4R3, F 31062 Toulouse Cedex, France
    Eur J Cell Biol 87:947-62. 2008
  2. ncbi Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
    Hideaki Tagami
    Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Cell 116:51-61. 2004
  3. pmc Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division
    Cheok Man Chow
    Gene Regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, London W12 0NN, UK
    EMBO Rep 6:354-60. 2005
  4. ncbi Expression patterns and post-translational modifications associated with mammalian histone H3 variants
    Sandra B Hake
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 281:559-68. 2006
  5. pmc Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 442:91-5. 2006
  6. ncbi PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state
    Alejandra Loyola
    CNRS UMR 218, Institut Curie, Paris 75248, France
    Mol Cell 24:309-16. 2006
  7. ncbi Organismal differences in post-translational modifications in histones H3 and H4
    Benjamin A Garcia
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA
    J Biol Chem 282:7641-55. 2007
  8. pmc H3.3/H2A.Z double variant-containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regions
    Chunyuan Jin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Genet 41:941-5. 2009
  9. pmc Distinct factors control histone variant H3.3 localization at specific genomic regions
    Aaron D Goldberg
    Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 140:678-91. 2010
  10. pmc The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3
    Pascal Drané
    IGMBC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch F 67400, France
    Genes Dev 24:1253-65. 2010

Research Grants

Scientific Experts

Detail Information

Publications132 found, 100 shown here

  1. doi HIV-1 Tat protein induces IL-10 production in monocytes by classical and alternative NF-kappaB pathways
    Kaoutar Leghmari
    Laboratoire d Immuno Virologie, EA 3038, Universite Paul Sabatier, 118, route de Narbonne, Bâtiment 4R3, F 31062 Toulouse Cedex, France
    Eur J Cell Biol 87:947-62. 2008
    ..assay experiments, after Tat treatment, revealed IKKalpha and CBP/p300 recruitment to the IL-10 promoter and histone H3 phosphorylation (Ser 10) and acetylation (Lys 14) in this region, presumably leading to chromatin remodeling...
  2. ncbi Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
    Hideaki Tagami
    Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Cell 116:51-61. 2004
    Deposition of the major histone H3 (H3.1) is coupled to DNA synthesis during DNA replication and possibly DNA repair, whereas histone variant H3.3 serves as the replacement variant for the DNA-synthesis-independent deposition pathway...
  3. pmc Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division
    Cheok Man Chow
    Gene Regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, London W12 0NN, UK
    EMBO Rep 6:354-60. 2005
    Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states...
  4. ncbi Expression patterns and post-translational modifications associated with mammalian histone H3 variants
    Sandra B Hake
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 281:559-68. 2006
    ..1 and H3.2 in mammalian cells should not be treated as equivalent proteins...
  5. pmc Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
    Haitao Li
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 442:91-5. 2006
    ..For example, histone H3 trimethylated at lysine 4 (H3K4me3) is associated with transcription start sites of active genes, but the ..
  6. ncbi PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state
    Alejandra Loyola
    CNRS UMR 218, Institut Curie, Paris 75248, France
    Mol Cell 24:309-16. 2006
    ..Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin...
  7. ncbi Organismal differences in post-translational modifications in histones H3 and H4
    Benjamin A Garcia
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA
    J Biol Chem 282:7641-55. 2007
    ..Using a combination of mass spectrometric and immunobiochemical approaches, we show that the PTM profile of histone H3 differs significantly among the various model organisms examined...
  8. pmc H3.3/H2A.Z double variant-containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regions
    Chunyuan Jin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    Nat Genet 41:941-5. 2009
    ..Other combinations of variants have different distributions, consistent with distinct roles for histone variants in the modulation of gene expression...
  9. pmc Distinct factors control histone variant H3.3 localization at specific genomic regions
    Aaron D Goldberg
    Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 140:678-91. 2010
    The incorporation of histone H3 variants has been implicated in the epigenetic memory of cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3...
  10. pmc The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3
    Pascal Drané
    IGMBC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch F 67400, France
    Genes Dev 24:1253-65. 2010
    The histone variant H3.3 marks active chromatin by replacing the conventional histone H3.1. In this study, we investigate the detailed mechanism of H3.3 replication-independent deposition...
  11. pmc Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres
    Peter W Lewis
    Laboratory of Chromatin Biology and Epigenetics, Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:14075-80. 2010
    ..These data demonstrate that Daxx functions as an H3.3-specific chaperone and facilitates the deposition of H3.3 at heterochromatin loci in the context of the ATRX-Daxx complex...
  12. doi Structures of human nucleosomes containing major histone H3 variants
    Hiroaki Tachiwana
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 162 8480, Japan
    Acta Crystallogr D Biol Crystallogr 67:578-83. 2011
    ..In humans, eight histone H3 variants, H3.1, H3.2, H3.3, H3T, H3.5, H3.X, H3.Y and CENP-A, have been reported to date...
  13. doi Dynamics of histone H3 deposition in vivo reveal a nucleosome gap-filling mechanism for H3.3 to maintain chromatin integrity
    Dominique Ray-Gallet
    Centre de Recherche, Institut Curie, 75248 Paris, France
    Mol Cell 44:928-41. 2011
    ..3, and HIRA association with Pol II ensures local H3.3 enrichment at specific sites. We discuss the importance of this H3.3 deposition as a salvage pathway to maintain chromatin integrity...
  14. doi Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma
    Jeremy Schwartzentruber
    McGill University and Genome Quebec Innovation Centre, Montreal, Quebec H3A 1A4, Canada
    Nature 482:226-31. 2012
    ..This is, to our knowledge, the first report to highlight recurrent mutations in a regulatory histone in humans, and our data suggest that defects of the chromatin architecture underlie paediatric and young adult GBM pathogenesis...
  15. pmc K27M mutation in histone H3.3 defines clinically and biologically distinct subgroups of pediatric diffuse intrinsic pontine gliomas
    Dong Anh Khuong-Quang
    Department of Human Genetics, McGill University, Montreal, QC, Canada
    Acta Neuropathol 124:439-47. 2012
    ..Here, we investigated clinical and biological impacts of histone H3.3 mutations. Forty-two DIPGs were tested for H3.3 mutations. Wild-type versus mutated (K27M-H3...
  16. pmc DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognition
    Simon J Elsässer
    Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, New York 10065, USA
    Nature 491:560-5. 2012
    ..3. Here we report the crystal structures of the DAXX histone-binding domain with a histone H3.3-H4 dimer, including mutants within DAXX and H3...
  17. doi Hotspot mutations in H3F3A and IDH1 define distinct epigenetic and biological subgroups of glioblastoma
    Dominik Sturm
    Division of Pediatric Neurooncology, German Cancer Research Center DKFZ Heidelberg, 69120 Heidelberg, Germany
    Cancer Cell 22:425-37. 2012
    ..We have recently identified recurrent H3F3A mutations affecting two critical amino acids (K27 and G34) of histone H3.3 in one-third of pediatric GBM...
  18. pmc Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXX
    Chao Pei Liu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
    Nat Struct Mol Biol 19:1287-92. 2012
    Mammalian histone H3.3 is a variant of the canonical H3.1 essential for genome reprogramming in fertilized eggs and maintenance of chromatin structure in neuronal cells. An H3...
  19. pmc DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatin
    Erwan Delbarre
    Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
    Genome Res 23:440-51. 2013
    ..3-H4) dimer rather than as a tetramer. Our data support a model of DAXX-mediated recruitment of (H3.3-H4) dimers to PML bodies, which may function as triage centers for H3.3 deposition into chromatin by distinct chaperones...
  20. doi H3.3 G34R mutations in pediatric primitive neuroectodermal tumors of central nervous system (CNS-PNET) and pediatric glioblastomas: possible diagnostic and therapeutic implications?
    Marco Gessi
    Institute of Neuropathology, University of Bonn Medical Center, Sigmund Freud Strasse 25, 53127 Bonn, Germany
    J Neurooncol 112:67-72. 2013
    ..recently have been exon sequenced with evidence that approximately 30 % of cases harbour mutations of the histone H3.3 gene...
  21. pmc Evaluation of histone 3 lysine 27 trimethylation (H3K27me3) and enhancer of Zest 2 (EZH2) in pediatric glial and glioneuronal tumors shows decreased H3K27me3 in H3F3A K27M mutant glioblastomas
    Sriram Venneti
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
    Brain Pathol 23:558-64. 2013
    ..Sixteen genes encode histone H3, each variant differing in only a few amino acids...
  22. doi H3F3A K27M mutation in pediatric CNS tumors: a marker for diffuse high-grade astrocytomas
    Gerrit H Gielen
    Institute of Neuropathology, University of Bonn Medical Center, Sigmund Freud Strasse 25, D 53105 Bonn, Germany
    Am J Clin Pathol 139:345-9. 2013
    ..Exon sequencing has identified a mutation in K27M of the histone H3.3 gene (H3F3A K27M and G34R/V) in about 20% of pediatric glioblastomas, but it remains to be seen whether these ..
  23. pmc Inhibition of PRC2 activity by a gain-of-function H3 mutation found in pediatric glioblastoma
    Peter W Lewis
    Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065, USA
    Science 340:857-61. 2013
    ..pediatric gliomas has identified missense mutations Lys27Met (K27M) and Gly34Arg/Val (G34R/V) in genes encoding histone H3.3 (H3F3A) and H3.1 (HIST3H1B)...
  24. pmc The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression
    Kui Ming Chan
    Department of Biochemistry and Molecular Biology, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 27:985-90. 2013
    ..have identified a Lys 27-to-methionine (K27M) mutation at one allele of H3F3A, one of the two genes encoding histone H3 variant H3.3, in 60% of high-grade pediatric glioma cases...
  25. doi Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas
    Sebastian Bender
    Division of Pediatric Neurooncology, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital, 69120 Heidelberg, Germany
    Cancer Cell 24:660-72. 2013
    ....
  26. pmc H3F3A K27M mutations in thalamic gliomas from young adult patients
    Koki Aihara
    Corresponding authors Akitake Mukasa, MD, PhD, Department of Neurosurgery, The University of Tokyo Hospital, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan Hiroyuki Aburatani, MD, PhD, Genome Science Division, Research Center for Advanced Science and Technology RCAST, The University of Tokyo, 4 6 1 Komaba, Meguro ku, Tokyo 153 8904, Japan
    Neuro Oncol 16:140-6. 2014
    Mutations in H3F3A, which encodes histone H3.3, commonly occur in pediatric glioblastoma...
  27. doi Mislocalization of the centromeric histone variant CenH3/CENP-A in human cells depends on the chaperone DAXX
    Nicolas Lacoste
    Institut Curie, Centre de Recherche, Paris75248, France CNRS, UMR3664, Paris75248, France Equipe Labellisée Ligue Contre le Cancer, UMR3664, Paris 75248, France UPMC, UMR3664, Paris 75248, France
    Mol Cell 53:631-44. 2014
    ..Our findings illustrate how changes in histone variant levels can disrupt chromatin dynamics and suggests a possible mechanism for cell resistance to anticancer treatments. ..
  28. ncbi Arsenite-induced phosphorylation of histone H3 at serine 10 is mediated by Akt1, extracellular signal-regulated kinase 2, and p90 ribosomal S6 kinase 2 but not mitogen- and stress-activated protein kinase 1
    Zhiwei He
    Hormel Institute, University of Minnesota, Austin 55912, USA
    J Biol Chem 278:10588-93. 2003
    ..Here, we provide evidence that arsenite can induce phosphorylation of histone H3 at serine 10 in a time- and dose-dependent manner in JB6 Cl 41 cells...
  29. pmc The good, the bad and the ugly: epigenetic mechanisms in glioblastoma
    Helena Caren
    UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, United Kingdom
    Mol Aspects Med 34:849-62. 2013
    ..3/H3F3A. These constitute the 'good, the bad and the ugly' of epigenetic mechanisms in cancer...
  30. pmc The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin components
    Monica Soldi
    Department of Experimental Oncology, European Institute of Oncology IEO, Via Adamello 16, Milan, Italy
    Mol Cell Proteomics 12:764-80. 2013
    ..X and WICH, both enriched in silent domains. Chromatin proteomics expands the arsenal of tools for deciphering how all the distinct protein components act together to enforce a given region-specific chromatin status...
  31. pmc Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone
    Sam Behjati
    1 Cancer Genome Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK 2 Department of Paediatrics, University of Cambridge, Cambridge, UK 3
    Nat Genet 45:1479-82. 2013
    ..Here, however, we report exquisite tumor type specificity for different histone H3.3 driver alterations. In 73 of 77 cases of chondroblastoma (95%), we found p...
  32. pmc A Cul4 E3 ubiquitin ligase regulates histone hand-off during nucleosome assembly
    Junhong Han
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Cell 155:817-29. 2013
    ..Here, we show that yeast Rtt101(Mms1) E3 ubiquitin ligase preferentially binds and ubiquitylates new histone H3 acetylated at lysine 56...
  33. doi Molecular genetics of gliomas
    Christina L Appin
    From the Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA
    Cancer J 20:66-72. 2014
    ..Other lower-grade gliomas of childhood, such as pilocytic astrocytoma and pleomorphic xanthoastrocytoma, are characterized by BRAF mutations or activating gene rearrangements involving BRAF. ..
  34. doi Giant cell tumor of bone: current treatment options
    Keith M Skubitz
    Department of Medicine, University of Minnesota Medical School, and the Masonic Cancer Center, Box 286, University Hospital, Minneapolis, MN, USA
    Curr Treat Options Oncol 15:507-18. 2014
    ..the malignant cell appears to be a mutation in H3F3A leading to a substitution of Gly34 to either Trp or Leu in Histone H3.3. This change presumably alters the methylation of the protein, and thus, its effect on gene expression...
  35. doi The highly prevalent H3F3A mutation in giant cell tumours of bone is not shared by sporadic central giant cell lesion of the jaws
    Carolina Cavalieri Gomes
    Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    Oral Surg Oral Med Oral Pathol Oral Radiol 118:583-5. 2014
    ..Gly34 Trp or p.Gly34 Leu in H3F3A, one of two genes for histone H3.3 located on chromosome 1...
  36. doi Altered global histone-trimethylation code and H3F3A-ATRX mutation in pediatric GBM
    Pankaj Pathak
    Department of Pathology, All India Institute of Medical Sciences AIIMS, New Delhi, India
    J Neurooncol 121:489-97. 2015
    ..Hence therapies for patients with particular combinations of histone modifications present opportunity to design innovative patient-tailored treatment protocols...
  37. pmc ATRX tolerates activity-dependent histone H3 methyl/phos switching to maintain repetitive element silencing in neurons
    Kyung Min Noh
    Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065
    Proc Natl Acad Sci U S A 112:6820-7. 2015
    ..g., histone H3 lysine 9 tri-methylation (H3K9me3)]...
  38. pmc Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeres
    Maheshi Udugama
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
    Nucleic Acids Res 43:10227-37. 2015
    ..3K9 trimethylation in heterochromatin formation at telomeres. It provides insights into H3.3 function in maintaining integrity of mammalian constitutive heterochromatin, adding to its role in mediating transcription memory in the genome. ..
  39. pmc Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypes
    David Castel
    UMR8203 Vectorologie et Thérapeutiques Anticancéreuses, CNRS, Gustave Roussy, Univ Paris Sud, Université Paris Saclay, 94805, Villejuif, France
    Acta Neuropathol 130:815-27. 2015
    ..b>Histone H3 genes mutations were assessed by immunochemistry and direct sequencing, whilst global gene expression profiling ..
  40. pmc Symplastic/pseudoanaplastic giant cell tumor of the bone
    Judy Sarungbam
    Department of Pathology, Memorial Sloan Kettering Cancer Center MSKCC, 1275 York Avenue, New York, NY, 10065, USA
    Skeletal Radiol 45:929-35. 2016
    ..Recently, somatic driver mutations of histone H3.3 exclusively in H3F3A have been described in GCTB...
  41. doi K27M mutation in H3F3A in ganglioglioma grade I with spontaneous malignant transformation extends the histopathological spectrum of the histone H3 oncogenic pathway
    Natacha Joyon
    Neuropathologie, Laboratoire Escourolle, Hôpitaux Universitaires Pitié Salpêtrière Charles Foix, AP HP, Paris, France
    Neuropathol Appl Neurobiol . 2016
    ..The most frequent genetic alterations in ganglioglioma are BRAF p.V600E mutation (40-60%), gain of chromosomes 7 (23%) and 5 (18%) (1-4). This article is protected by copyright. All rights reserved...
  42. ncbi Pediatric thalamic glioma with H3F3A K27M mutation, which was detected before and after malignant transformation: a case report
    Kenichi Ishibashi
    Department of Neurosurgery, Osaka City General Hospital, 2 13 22 Miyakojima Hondori, Miyakojima ku, Osaka, 534 0021, Japan
    Childs Nerv Syst 32:2433-2438. 2016
    b>Histone H3.3 (H3F3A) mutation in the codon for lysine 27 (K27M) has been found as driver mutations in pediatric glioblastoma and has been suggested to play critical roles in the pathogenesis of thalamic gliomas and diffuse intrinsic ..
  43. ncbi Clinical, Imaging, Histopathological and Molecular Characterization of Anaplastic Ganglioglioma
    Marc Zanello
    From the Department of Neurosurgery MZ, ED, Bd, JP, Department of Neuropathology, Sainte Anne Hospital, Paris, France MP, AT E, FC, FA, PV, Department of Biochemistry, Paul Brousse Hospital, Paris, France RS Department of Pediatric Neurosurgery, Necker Enfants Malades Hospital, Paris, France SP, CS R, MZ, Paris Descartes University, Paris, France MZ, MP, AT E, SP, ED, Bd, FC, FA, CS R, MZ, J FM, JP, PV, Department of Medical Biology and Pathology LL, Department of Neurooncology, UK
    J Neuropathol Exp Neurol 75:971-980. 2016
    ..K27M H3F3A mutation (17%). AGGs are malignant neoplasms requiring aggressive oncological treatment. In the perspective of targeted therapies, AGGs should be screened for BRAF V600E, hTERT, ATRX, and mutations of histone genes...
  44. doi Histone variant H3F3A promotes lung cancer cell migration through intronic regulation
    Seong Min Park
    Specific Organs Cancer Branch, Research Institute, National Cancer Center, Goyang, Gyeonggi 10408, Republic of Korea
    Nat Commun 7:12914. 2016
    Although several somatic single nucleotide variations in histone H3.3 have been investigated as cancer drivers, other types of aberration have not been well studied. Here, we demonstrate that overexpression of H3F3A, encoding H3...
  45. doi H3F3 mutation status of giant cell tumors of the bone, chondroblastomas and their mimics: a combined high resolution melting and pyrosequencing approach
    Thibault Kervarrec
    Department of Pathology, University Hospital of Tours, avenue de la Republique, Chambray les Tours, France
    Mod Pathol . 2017
    Behjati et al recently described recurrent mutations of H3F3 genes in giant cell tumors of the bone and chondroblastomas...
  46. pmc The role of histone modifications and telomere alterations in the pathogenesis of diffuse gliomas in adults and children
    Julieann Lee
    Division of Neuropathology, Department of Pathology, University of California, San Francisco, 505 Parnassus Ave, Room M 551, Box 0102, San Francisco, CA, 94143, USA
    J Neurooncol . 2017
    ..Mutations including K27M and G34R/V that affect critical amino acids within the N-terminal tail of the histone H3 variants, H3.3 and H3...
  47. doi H3.5 is a novel hominid-specific histone H3 variant that is specifically expressed in the seminiferous tubules of human testes
    Raphael Schenk
    HELIOS Children s Hospital, HELIOS Klinikum Wuppertal, Research Laboratory, Witten Herdecke University, Germany
    Chromosoma 120:275-85. 2011
    ..Six human histone H3 variants are known to date, not counting CenH3 variants: H3.1, H3.2, H3.3 and the testis-specific H3...
  48. pmc Levels of potential oral cancer salivary mRNA biomarkers in oral cancer patients in remission and oral lichen planus patients
    Yi Shing Lisa Cheng
    Department of Diagnostic Sciences, Texas A and M University Baylor College of Dentistry, 3302 Gaston Ave, Dallas, TX, USA
    Clin Oral Investig 18:985-93. 2014
    ....
  49. doi Discovery, identification and sequence analysis of RNAs selected for very short or long poly A tail in immature bovine oocytes
    Maella Gohin
    Centre de Recherche en Biologie de la Reproduction, Faculté des sciences de l agriculture et de l alimentation, Departement des Sciences Animales, 2440 Bl Hochelaga, Pavillon INAF, Universite Laval, Quebec, QC, Canada G1V 0A6
    Mol Hum Reprod 20:127-38. 2014
    ..Moreover, we identified deadenylated RNAs in bovine oocytes that may be stored for the long-term process of early embryo development and described a conserved motif enriched in the 3'UTR of deadenylated RNAs...
  50. pmc The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomes
    Robert Huether
    1 Department of Computational Biology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA 2
    Nat Commun 5:3630. 2014
    ..Collectively, our results help to define the landscape of mutations in epigenetic regulatory genes in paediatric cancer and yield a valuable new database for investigating the role of epigenetic dysregulations in cancer. ..
  51. pmc Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
    Yasuhiro Arimura
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Sci Rep 4:7115. 2014
    The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3...
  52. pmc Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues
    Chiara Romani
    Angelo Nocivelli Institute of Molecular Medicine, Division of Gynecologic Oncology, University of Brescia, Brescia, Italy
    PLoS ONE 9:e113781. 2014
    ..In conclusion, our results recommend the use of PPIA as a single reference gene to be considered for improved reliability of normalization in gene expression studies involving endometrial tumor samples at different tumor degrees. ..
  53. pmc Contribution of the two genes encoding histone variant h3.3 to viability and fertility in mice
    Michelle C W Tang
    Department of Zoology, The University of Melbourne, Melbourne, Victoria, Australia Genetics Theme, Murdoch Children s Research Institute, Parkville, Victoria, Australia
    PLoS Genet 11:e1004964. 2015
    ..Much remains to be learned of H3.3 functions in germ cell development. Histone H3.3 presents a unique genetic paradigm in that two conventional intron-containing genes encode the identical ..
  54. doi The activities of four constitutively expressed promoters in single-copy transgenic rice plants for two homozygous generations
    Seung Woon Bang
    Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Pyeongchang, 232 916, Korea
    Planta 241:1529-41. 2015
    We have characterized four novel constitutive promoters ARP1, H3F3, HSP and H2BF3 that are active in all tissues/stages of transgenic plants and stable over two homozygous generations...
  55. doi Diagnostic, prognostic and predictive relevance of molecular markers in gliomas
    Sebastian Brandner
    Division of Neuropathology, The National Hospital for Neurology and Neurosurgery, University College London NHS Foundation Trust, London, UK
    Neuropathol Appl Neurobiol 41:694-720. 2015
    ..In a tumour-specific topic, the role of mitogen-activated protein kinase pathway mutations in the pathogenesis of pilocytic astrocytomas will be covered. ..
  56. doi Biomarker-driven diagnosis of diffuse gliomas
    Christina L Appin
    Department of Pathology and Laboratory Medicine, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA
    Mol Aspects Med 45:87-96. 2015
    ..These gliomas often harbor mutations or activating gene rearrangements in BRAF. ..
  57. pmc Detecting the H3F3A mutant allele found in high-grade pediatric glioma by real-time PCR
    Ray Zhang
    Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 1st SW, Rochester, MN, 55905, USA
    J Neurooncol 126:27-36. 2016
    ..80% of DIPG cases and 70% of midline glioblastomas contain a mutation at one allele of the H3F3A gene (encoding histone H3 variant H3.3), replacing the lysine 27 with methionine (K27M)...
  58. doi Mutation Analysis of H3F3A and H3F3B as a Diagnostic Tool for Giant Cell Tumor of Bone and Chondroblastoma
    Arjen H G Cleven
    Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
    Am J Surg Pathol 39:1576-83. 2015
    ....
  59. doi Diffuse Midline Gliomas with Histone H3-K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic Alterations
    David A Solomon
    Division of Neuropathology, Department of Pathology, University of California, San Francisco, CA
    Brain Pathol 26:569-80. 2016
    Somatic mutations of the H3F3A and HIST1H3B genes encoding the histone H3 variants, H3.3 and H3.1, were recently identified in high-grade gliomas arising in the thalamus, pons and spinal cord of children and young adults...
  60. doi Molecular classification of gliomas
    Kenta Masui
    Department of Pathology, Tokyo Women s Medical University, Shinjku ku, Tokyo, Japan Ludwig Institute for Cancer Research, University of California San Diego, La Jolla, CA, USA
    Handb Clin Neurol 134:97-120. 2016
    ..Finally, we illustrate how advances in molecular diagnostics affect novel strategies of targeted therapy, thereby raising new challenges and identifying new leads for personalized treatment of glioma patients. ..
  61. ncbi Molecular Diagnostic and Prognostic Subtyping of Gliomas in Tunisian Population
    Saoussen Trabelsi
    Department of Cytogenetics, Molecular Genetics and Reproductive Biology, Farhat Hached University Hospital, Street Ibn ELJAZZAR, 4000, Sousse, Tunisia
    Mol Neurobiol . 2016
    ..Furthermore, by exhibiting a remarkable methylation profile, it emphasised the oncogenic power of G34R mutation connecting gliomagenesis and chromatin regulation...
  62. doi [Molecular Genetics as Best Evidence in Glioma Diagnostics]
    Kenta Masui
    Department of Pathology, Tokyo Women s Medical University
    Brain Nerve 68:253-61. 2016
    ....
  63. pmc Identification of citrullinated peptides in the synovial fluid of patients with rheumatoid arthritis using LC-MALDI-TOF/TOF
    Fei Wang
    Institute of Clinical Laboratory Science, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China
    Clin Rheumatol 35:2185-94. 2016
    ..Interestingly, citrullinated histone H3.3 (H3F3A) was found in OA controls, but not in RA groups...
  64. doi Recent Advances on the Molecular Pathology of Glial Neoplasms in Children and Adults
    Fausto J Rodriguez
    Division of Neuropathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland Electronic address
    J Mol Diagn 18:620-34. 2016
    ..Here, we summarize current concepts in molecular testing for glial tumors, including recent findings by large-scale discovery efforts and technologic advances that are affecting routine diagnostic work. ..
  65. doi Co-occurrence of Histone H3 K27M and BRAF V600E mutations in paediatric midline grade I ganglioglioma
    Mélanie Pages
    Department of Neuropathology, Sainte Anne Hospital, Paris, France
    Brain Pathol . 2016
    ..This article is protected by copyright. All rights reserved...
  66. doi Bithalamic Gliomas May Be Molecularly Distinct From Their Unilateral High-Grade Counterparts
    Alberto Broniscer
    Department of Oncology, St Jude Children s Research Hospital, Memphis, TN, USA
    Brain Pathol . 2016
    ..This finding suggests that unilateral and bithalamic high-grade gliomas may represent two distinct molecular entities. This article is protected by copyright. All rights reserved...
  67. pmc Recurrent somatic alterations of FGFR1 and NTRK2 in pilocytic astrocytoma
    David T W Jones
    Division of Pediatric Neurooncology, German Cancer Research Center DKFZ, Heidelberg, Germany
    Nat Genet 45:927-32. 2013
    ..Our findings thus identify new potential therapeutic targets in distinct subsets of pilocytic astrocytoma and childhood glioblastoma. ..
  68. doi Molecular neuro-oncology in clinical practice: a new horizon
    Michael Weller
    Department of Neurology, University Hospital Zurich, Zurich, Switzerland
    Lancet Oncol 14:e370-9. 2013
    ..large-scale molecular profiling approaches have identified new mutations in gliomas, affecting IDH1, IDH2, H3F3, ATRX, and CIC, which has allowed subclassification of gliomas into distinct molecular subgroups with ..
  69. pmc Comprehensive analysis of transcriptome variation uncovers known and novel driver events in T-cell acute lymphoblastic leukemia
    Zeynep Kalender Atak
    Laboratory of Computational Biology, Center for Human Genetics, KU Leuven, Leuven, Belgium
    PLoS Genet 9:e1003997. 2013
    ....
  70. pmc Di- and tri- but not monomethylation on histone H3 lysine 36 marks active transcription of genes involved in flowering time regulation and other processes in Arabidopsis thaliana
    Lin Xu
    Institut de Biologie Moléculaire des Plantes CNRS, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
    Mol Cell Biol 28:1348-60. 2008
    ..Together our results reveal that the deposition of H3K36 methylation is finely regulated, possibly to cope with the complex regulation of growth and development in higher eukaryotes...
  71. pmc Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly
    Bin Kang
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Genes Dev 25:1359-64. 2011
    b>Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3...
  72. pmc Recognition of unmodified histone H3 by the first PHD finger of bromodomain-PHD finger protein 2 provides insights into the regulation of histone acetyltransferases monocytic leukemic zinc-finger protein (MOZ) and MOZ-related factor (MORF)
    Su Qin
    Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
    J Biol Chem 286:36944-55. 2011
    ..Here we show that the first PHD finger of BRPF2 specifically recognizes the N-terminal tail of unmodified histone H3 (unH3) and report the solution structures of this PHD finger both free and in complex with the unH3 peptide...
  73. pmc A comparative molecular dynamics study of methylation state specificity of JMJD2A
    Ozlem Ulucan
    Center for Computational Biology and Bioinformatics and College of Engineering, Koc University, Istanbul, Turkey
    PLoS ONE 6:e24664. 2011
    ..Our study provides new insights into how JMJD2A discriminates between its substrates from both a structural and dynamical point of view...
  74. pmc Histone chaperone FACT coordinates nucleosome interaction through multiple synergistic binding events
    Duane D Winkler
    Howard Hughes Medical Institute and the Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523 1870, USA
    J Biol Chem 286:41883-92. 2011
    ..Together, the data reveal that specific FACT subunits synchronize interactions with various target sites on individual nucleosomes to generate a high affinity binding event and promote reorganization...
  75. pmc CBX3 regulates efficient RNA processing genome-wide
    Andrea Smallwood
    Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
    Genome Res 22:1426-36. 2012
    ..In addition, we observed defective recruitment of splicing factors, including SNRNP70, to CBX3 target genes. Collectively, our data suggest a role for CBX3 in aiding in efficient cotranscriptional RNA processing...
  76. doi Frequent ATRX mutations and loss of expression in adult diffuse astrocytic tumors carrying IDH1/IDH2 and TP53 mutations
    Xiao Yang Liu
    Department of Human Genetics, McGill University, Montreal, QC, Canada
    Acta Neuropathol 124:615-25. 2012
    ..Combined alteration of these genes may contribute to drive the neoplastic growth in a major subset of diffuse astrocytomas in adults...
  77. doi CLN5 and CLN8 protein association with ceramide synthase: biochemical and proteomic approaches
    Saria El Haddad
    Department of Pediatric, American University of Beirut, Beirut, Lebanon
    Electrophoresis 33:3798-809. 2012
    ..We explore the role of the CLN5/CLN8 proteins in ceramide species specific sphingolipid de novo synthesis, and suggest that CLN5/CLN8 proteins are more closely related than previously believed...
  78. pmc Mutations in SETD2 and genes affecting histone H3K36 methylation target hemispheric high-grade gliomas
    Adam M Fontebasso
    Division of Experimental Medicine, McGill University and McGill University Health Centre, Montreal, QC, Canada
    Acta Neuropathol 125:659-69. 2013
    Recurrent mutations affecting the histone H3.3 residues Lys27 or indirectly Lys36 are frequent drivers of pediatric high-grade gliomas (over 30% of HGGs)...
  79. pmc Whole-genome sequencing identifies genetic alterations in pediatric low-grade gliomas
    Jinghui Zhang
    Department of Computational Biology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
    Nat Genet 45:602-12. 2013
    ..Focusing on the therapeutically challenging diffuse LGGs, our study of 151 tumors has discovered genetic alterations and potential therapeutic targets across the entire range of pediatric LGGs and LGGNTs...
  80. pmc G34, another connection between MYCN and a pediatric tumor
    Miller Huang
    University of California San Francisco, San Francisco, CA, USA
    Cancer Discov 3:484-6. 2013
    ..In this issue of Cancer Discovery, Bjerke and colleagues identify mutations at G34 in H3F3A that result in elevated expression of MYCN as a potential mechanism in gliomagenesis...
  81. pmc Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs
    D Wells
    Proc Natl Acad Sci U S A 82:2834-8. 1985
    ..Hybridization of subsegments of the cDNA to human genomic DNA reveals a complex multigene family. The differences in the structures of basal and cell-cycle histone genes suggest a model to explain the differences in their expression...
  82. pmc Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene family
    D Wells
    Nucleic Acids Res 15:2871-89. 1987
    ..We also present the sequence of three related processed human H3.3 pseudogenes and provide evidence demonstrating that most of the 20 to 30 copies of the H3.3 gene within the human genome are in fact processed pseudogenes...
  83. ncbi Human spleen histone H3. Isolation and amino acid sequence
    Y Ohe
    J Biochem 90:1205-11. 1981
    The amino acid sequence of human spleen histone H3 was investigated as a study in histone evolution, following previous investigations of human spleen histone H2B [Ohe, Y., Hayashi, H., & Iwai, K. (1979) J. Biochem...
  84. ncbi The human replacement histone H3.3B gene (H3F3B)
    W Albig
    Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat Gottingen, Germany
    Genomics 30:264-72. 1995
    ..This localization of the H3.3B gene and its solitary arrangement contrast with the majority of the replication-dependent histone genes, which form a large cluster on chromosome 6 and a second cluster on chromosome 1...
  85. ncbi The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3
    A Palaparti
    Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
    J Biol Chem 272:26604-10. 1997
    ..and far Western blotting studies show further that native Groucho/TLE proteins interact specifically with histone H3 and not with other core histones...
  86. ncbi Localization of the human H3F3A histone gene to 1q41, outside of the normal histone gene clusters
    X Lin
    Department of Biology, University of Houston, Texas 77204, USA
    Genomics 46:526-8. 1997
  87. ncbi Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation
    H Goto
    Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
    J Biol Chem 274:25543-9. 1999
    b>Histone H3 (H3) phosphorylation at Ser(10) occurs during mitosis in eukaryotes and was recently shown to play an important role in chromosome condensation in Tetrahymena...
  88. ncbi Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14
    W S Lo
    Molecular Genetics Program, Wistar Institute, Philadelphia, Pennsylvania 19024, USA
    Mol Cell 5:917-26. 2000
    ..We examined the relationship between serine 10 phosphorylation and lysine 14 acetylation in histone H3 and have found that, in vitro, several HAT enzymes displayed increased activity on H3 peptides bearing phospho-..
  89. ncbi Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
    M Lachner
    Research Institute of Molecular Pathology, The Vienna Biocenter, Vienna, Austria
    Nature 410:116-20. 2001
    ..Here we show that mammalian methyltransferases that selectively methylate histone H3 on lysine 9 (Suv39h HMTases) generate a binding site for HP1 proteins--a family of heterochromatic adaptor ..
  90. ncbi MAP kinases mediate UVB-induced phosphorylation of histone H3 at serine 28
    S Zhong
    Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
    J Biol Chem 276:12932-7. 2001
    b>Histone H3 phosphorylation is related closely to chromatin remodeling and chromosome condensation. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines...
  91. ncbi Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails
    Y Zhang
    Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
    Genes Dev 15:2343-60. 2001
  92. pmc Methylation at arginine 17 of histone H3 is linked to gene activation
    Uta Maria Bauer
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    EMBO Rep 3:39-44. 2002
    The nuclear hormone receptor co-activator CARM1 has the potential to methylate histone H3 at arginine residues in vitro. The methyltransferase activity of CARM1 is necessary for its co-activator functions in transient transfection assays...
  93. ncbi Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex
    Philip Zegerman
    Wellcome Cancer Research UK Institute, Tennis Court Rd, Cambridge, United Kingdom
    J Biol Chem 277:11621-4. 2002
    ..proteins, such as GCN5, bind acetyl lysines and the chromodomain protein, HP1, binds methyl lysine 9 of histone H3. Here we show that the previously characterized transcriptional repressor complex NuRD (nucleosome remodeling ..
  94. ncbi Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation
    Hidemasa Goto
    Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi464 8681, Japan
    Genes Cells 7:11-7. 2002
    b>Histone H3 (H3) phosphorylation plays important roles in mitotic chromosome condensation. We reported that H3 phosphorylation occurs at Ser28, as well as at Ser10 during mitosis, at least in mammals...
  95. pmc Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase
    Ute Preuss
    Institute of Genetics, University of Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
    Nucleic Acids Res 31:878-85. 2003
    ..Interestingly, Dlk/ZIP kinase phosphorylated histone H3 at a novel site, Thr11, rather than Ser10, which is characteristic of mitotic chromosomes...
  96. pmc MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14
    Ana Soloaga
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
    EMBO J 22:2788-97. 2003
    ..by the rapid induction of immediate-early (IE) genes, which occurs concomitantly with the phosphorylation of histone H3 and the high-mobility-group protein HMG-14...
  97. ncbi Development of a competitive reverse-transcription polymerase chain reaction for quantitative measurement of sulfotransferase subfamily 1A enzyme in human hepatoma cells
    Hannelore Dassow
    Institute of Clinical Pharmacology, Faculty of Medicine, University of Leipzig, Leipzig, Germany
    Anal Biochem 319:314-7. 2003
  98. ncbi Differential expression of human replacement and cell cycle dependent H3 histone genes
    Derk Frank
    Institut fur Biochemie und Molekulare Zellbiologie, Abteilung Molekularbiologie, Universitat Gottingen, Humboldtallee 23, D 37073 Gottingen, Germany
    Gene 312:135-43. 2003
    ..3A gene expression. The possible functional relevance of the differences between the two H3.3 genes in structure and expression is discussed...
  99. ncbi Human PAD4 regulates histone arginine methylation levels via demethylimination
    Yanming Wang
    Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Science 306:279-83. 2004
    ..Moreover, PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells. These data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones...

Research Grants4

  1. Mechanism of Gene Silencing by DNA Methylation
    Yi Zhang; Fiscal Year: 2005
    ..The proposed studies will provide a thorough characterization of the MeCP1 complex. They will also reveal the potential targets and repression mechanism of the MeCP1 complex. ..
  2. Structure and Function of CTCF: Mouse Model Studies
    Galina Filippova; Fiscal Year: 2007
    ..1 where CTCF maps. The health-relatedness is that the identified mechanisms can be targeted for both cancer prevention and therapeutic intervention. ..
  3. Role of histone methyltransferases hDOTIL in leukemogenesis
    Yi Zhang; Fiscal Year: 2009
    ..domain, a signature HMTase motif, and their ability to methylate lysine 79 located within the globular domain of histone H3. Toward understanding the biological function of hDOTIL, we set out to identify its functional partners...
  4. Mechanisms of Polycomb Silencing
    Yi Zhang; Fiscal Year: 2010
    ..5. .Determine the transcriptional effect and understand the mechanism of H3K27 methylation and H2A ubiquitylation using a reconstituted chromatin transcription system. More description in proposal. ..