Genomes and Genes
Gene Symbol: histone H3
Description: H3 histone family member 3A
Alias: H3.3A, H3F3, histone H3.3, H3 histone, family 3A
Publications132 found, 100 shown here
- HIV-1 Tat protein induces IL-10 production in monocytes by classical and alternative NF-kappaB pathwaysKaoutar Leghmari
Laboratoire d Immuno Virologie, EA 3038, Universite Paul Sabatier, 118, route de Narbonne, Bâtiment 4R3, F 31062 Toulouse Cedex, France
Eur J Cell Biol 87:947-62. 2008..assay experiments, after Tat treatment, revealed IKKalpha and CBP/p300 recruitment to the IL-10 promoter and histone H3 phosphorylation (Ser 10) and acetylation (Lys 14) in this region, presumably leading to chromatin remodeling...
- Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesisHideaki Tagami
Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
Cell 116:51-61. 2004Deposition of the major histone H3 (H3.1) is coupled to DNA synthesis during DNA replication and possibly DNA repair, whereas histone variant H3.3 serves as the replacement variant for the DNA-synthesis-independent deposition pathway...
- Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell divisionCheok Man Chow
Gene Regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, London W12 0NN, UK
EMBO Rep 6:354-60. 2005Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states...
- Expression patterns and post-translational modifications associated with mammalian histone H3 variantsSandra B Hake
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 281:559-68. 2006..1 and H3.2 in mammalian cells should not be treated as equivalent proteins...
- Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURFHaitao Li
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 442:91-5. 2006..For example, histone H3 trimethylated at lysine 4 (H3K4me3) is associated with transcription start sites of active genes, but the ..
- PTMs on H3 variants before chromatin assembly potentiate their final epigenetic stateAlejandra Loyola
CNRS UMR 218, Institut Curie, Paris 75248, France
Mol Cell 24:309-16. 2006..Although the set of initial modifications present on H3.1 is permissive for further modifications, in H3.3 a subset cannot be K9me3. Thus, initial modifications impact final PTMs within chromatin...
- Organismal differences in post-translational modifications in histones H3 and H4Benjamin A Garcia
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA
J Biol Chem 282:7641-55. 2007..Using a combination of mass spectrometric and immunobiochemical approaches, we show that the PTM profile of histone H3 differs significantly among the various model organisms examined...
- H3.3/H2A.Z double variant-containing nucleosomes mark 'nucleosome-free regions' of active promoters and other regulatory regionsChunyuan Jin
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
Nat Genet 41:941-5. 2009..Other combinations of variants have different distributions, consistent with distinct roles for histone variants in the modulation of gene expression...
- Distinct factors control histone variant H3.3 localization at specific genomic regionsAaron D Goldberg
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Cell 140:678-91. 2010The incorporation of histone H3 variants has been implicated in the epigenetic memory of cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3...
- The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3Pascal Drané
IGMBC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch F 67400, France
Genes Dev 24:1253-65. 2010The histone variant H3.3 marks active chromatin by replacing the conventional histone H3.1. In this study, we investigate the detailed mechanism of H3.3 replication-independent deposition...
- Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeresPeter W Lewis
Laboratory of Chromatin Biology and Epigenetics, Rockefeller University, 1230 York Ave, New York, NY 10065, USA
Proc Natl Acad Sci U S A 107:14075-80. 2010..These data demonstrate that Daxx functions as an H3.3-specific chaperone and facilitates the deposition of H3.3 at heterochromatin loci in the context of the ATRX-Daxx complex...
- Structures of human nucleosomes containing major histone H3 variantsHiroaki Tachiwana
Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 162 8480, Japan
Acta Crystallogr D Biol Crystallogr 67:578-83. 2011..In humans, eight histone H3 variants, H3.1, H3.2, H3.3, H3T, H3.5, H3.X, H3.Y and CENP-A, have been reported to date...
- Dynamics of histone H3 deposition in vivo reveal a nucleosome gap-filling mechanism for H3.3 to maintain chromatin integrityDominique Ray-Gallet
Centre de Recherche, Institut Curie, 75248 Paris, France
Mol Cell 44:928-41. 2011..3, and HIRA association with Pol II ensures local H3.3 enrichment at specific sites. We discuss the importance of this H3.3 deposition as a salvage pathway to maintain chromatin integrity...
- Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastomaJeremy Schwartzentruber
McGill University and Genome Quebec Innovation Centre, Montreal, Quebec H3A 1A4, Canada
Nature 482:226-31. 2012..This is, to our knowledge, the first report to highlight recurrent mutations in a regulatory histone in humans, and our data suggest that defects of the chromatin architecture underlie paediatric and young adult GBM pathogenesis...
- K27M mutation in histone H3.3 defines clinically and biologically distinct subgroups of pediatric diffuse intrinsic pontine gliomasDong Anh Khuong-Quang
Department of Human Genetics, McGill University, Montreal, QC, Canada
Acta Neuropathol 124:439-47. 2012..Here, we investigated clinical and biological impacts of histone H3.3 mutations. Forty-two DIPGs were tested for H3.3 mutations. Wild-type versus mutated (K27M-H3...
- DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognitionSimon J Elsässer
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, New York 10065, USA
Nature 491:560-5. 2012..3. Here we report the crystal structures of the DAXX histone-binding domain with a histone H3.3-H4 dimer, including mutants within DAXX and H3...
- Hotspot mutations in H3F3A and IDH1 define distinct epigenetic and biological subgroups of glioblastomaDominik Sturm
Division of Pediatric Neurooncology, German Cancer Research Center DKFZ Heidelberg, 69120 Heidelberg, Germany
Cancer Cell 22:425-37. 2012..We have recently identified recurrent H3F3A mutations affecting two critical amino acids (K27 and G34) of histone H3.3 in one-third of pediatric GBM...
- Structure of the variant histone H3.3-H4 heterodimer in complex with its chaperone DAXXChao Pei Liu
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
Nat Struct Mol Biol 19:1287-92. 2012Mammalian histone H3.3 is a variant of the canonical H3.1 essential for genome reprogramming in fertilized eggs and maintenance of chromatin structure in neuronal cells. An H3...
- DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending deposition into chromatinErwan Delbarre
Stem Cell Epigenetics Laboratory, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway
Genome Res 23:440-51. 2013..3-H4) dimer rather than as a tetramer. Our data support a model of DAXX-mediated recruitment of (H3.3-H4) dimers to PML bodies, which may function as triage centers for H3.3 deposition into chromatin by distinct chaperones...
- H3.3 G34R mutations in pediatric primitive neuroectodermal tumors of central nervous system (CNS-PNET) and pediatric glioblastomas: possible diagnostic and therapeutic implications?Marco Gessi
Institute of Neuropathology, University of Bonn Medical Center, Sigmund Freud Strasse 25, 53127 Bonn, Germany
J Neurooncol 112:67-72. 2013..recently have been exon sequenced with evidence that approximately 30 % of cases harbour mutations of the histone H3.3 gene...
- Evaluation of histone 3 lysine 27 trimethylation (H3K27me3) and enhancer of Zest 2 (EZH2) in pediatric glial and glioneuronal tumors shows decreased H3K27me3 in H3F3A K27M mutant glioblastomasSriram Venneti
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Brain Pathol 23:558-64. 2013..Sixteen genes encode histone H3, each variant differing in only a few amino acids...
- H3F3A K27M mutation in pediatric CNS tumors: a marker for diffuse high-grade astrocytomasGerrit H Gielen
Institute of Neuropathology, University of Bonn Medical Center, Sigmund Freud Strasse 25, D 53105 Bonn, Germany
Am J Clin Pathol 139:345-9. 2013..Exon sequencing has identified a mutation in K27M of the histone H3.3 gene (H3F3A K27M and G34R/V) in about 20% of pediatric glioblastomas, but it remains to be seen whether these ..
- Inhibition of PRC2 activity by a gain-of-function H3 mutation found in pediatric glioblastomaPeter W Lewis
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065, USA
Science 340:857-61. 2013..pediatric gliomas has identified missense mutations Lys27Met (K27M) and Gly34Arg/Val (G34R/V) in genes encoding histone H3.3 (H3F3A) and H3.1 (HIST3H1B)...
- The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expressionKui Ming Chan
Department of Biochemistry and Molecular Biology, University of California at San Francisco, San Francisco, California 94143, USA
Genes Dev 27:985-90. 2013..have identified a Lys 27-to-methionine (K27M) mutation at one allele of H3F3A, one of the two genes encoding histone H3 variant H3.3, in 60% of high-grade pediatric glioma cases...
- Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomasSebastian Bender
Division of Pediatric Neurooncology, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany Department of Pediatric Oncology, Hematology, and Immunology, Heidelberg University Hospital, 69120 Heidelberg, Germany
Cancer Cell 24:660-72. 2013....
- H3F3A K27M mutations in thalamic gliomas from young adult patientsKoki Aihara
Corresponding authors Akitake Mukasa, MD, PhD, Department of Neurosurgery, The University of Tokyo Hospital, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan Hiroyuki Aburatani, MD, PhD, Genome Science Division, Research Center for Advanced Science and Technology RCAST, The University of Tokyo, 4 6 1 Komaba, Meguro ku, Tokyo 153 8904, Japan
Neuro Oncol 16:140-6. 2014Mutations in H3F3A, which encodes histone H3.3, commonly occur in pediatric glioblastoma...
- Mislocalization of the centromeric histone variant CenH3/CENP-A in human cells depends on the chaperone DAXXNicolas Lacoste
Institut Curie, Centre de Recherche, Paris75248, France CNRS, UMR3664, Paris75248, France Equipe Labellisée Ligue Contre le Cancer, UMR3664, Paris 75248, France UPMC, UMR3664, Paris 75248, France
Mol Cell 53:631-44. 2014..Our findings illustrate how changes in histone variant levels can disrupt chromatin dynamics and suggests a possible mechanism for cell resistance to anticancer treatments. ..
- Arsenite-induced phosphorylation of histone H3 at serine 10 is mediated by Akt1, extracellular signal-regulated kinase 2, and p90 ribosomal S6 kinase 2 but not mitogen- and stress-activated protein kinase 1Zhiwei He
Hormel Institute, University of Minnesota, Austin 55912, USA
J Biol Chem 278:10588-93. 2003..Here, we provide evidence that arsenite can induce phosphorylation of histone H3 at serine 10 in a time- and dose-dependent manner in JB6 Cl 41 cells...
- The good, the bad and the ugly: epigenetic mechanisms in glioblastomaHelena Caren
UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, United Kingdom
Mol Aspects Med 34:849-62. 2013..3/H3F3A. These constitute the 'good, the bad and the ugly' of epigenetic mechanisms in cancer...
- The proteomic investigation of chromatin functional domains reveals novel synergisms among distinct heterochromatin componentsMonica Soldi
Department of Experimental Oncology, European Institute of Oncology IEO, Via Adamello 16, Milan, Italy
Mol Cell Proteomics 12:764-80. 2013..X and WICH, both enriched in silent domains. Chromatin proteomics expands the arsenal of tools for deciphering how all the distinct protein components act together to enforce a given region-specific chromatin status...
- Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of boneSam Behjati
1 Cancer Genome Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK 2 Department of Paediatrics, University of Cambridge, Cambridge, UK 3
Nat Genet 45:1479-82. 2013..Here, however, we report exquisite tumor type specificity for different histone H3.3 driver alterations. In 73 of 77 cases of chondroblastoma (95%), we found p...
- A Cul4 E3 ubiquitin ligase regulates histone hand-off during nucleosome assemblyJunhong Han
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Cell 155:817-29. 2013..Here, we show that yeast Rtt101(Mms1) E3 ubiquitin ligase preferentially binds and ubiquitylates new histone H3 acetylated at lysine 56...
- Molecular genetics of gliomasChristina L Appin
From the Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA
Cancer J 20:66-72. 2014..Other lower-grade gliomas of childhood, such as pilocytic astrocytoma and pleomorphic xanthoastrocytoma, are characterized by BRAF mutations or activating gene rearrangements involving BRAF. ..
- Giant cell tumor of bone: current treatment optionsKeith M Skubitz
Department of Medicine, University of Minnesota Medical School, and the Masonic Cancer Center, Box 286, University Hospital, Minneapolis, MN, USA
Curr Treat Options Oncol 15:507-18. 2014..the malignant cell appears to be a mutation in H3F3A leading to a substitution of Gly34 to either Trp or Leu in Histone H3.3. This change presumably alters the methylation of the protein, and thus, its effect on gene expression...
- The highly prevalent H3F3A mutation in giant cell tumours of bone is not shared by sporadic central giant cell lesion of the jawsCarolina Cavalieri Gomes
Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Oral Surg Oral Med Oral Pathol Oral Radiol 118:583-5. 2014..Gly34 Trp or p.Gly34 Leu in H3F3A, one of two genes for histone H3.3 located on chromosome 1...
- Altered global histone-trimethylation code and H3F3A-ATRX mutation in pediatric GBMPankaj Pathak
Department of Pathology, All India Institute of Medical Sciences AIIMS, New Delhi, India
J Neurooncol 121:489-97. 2015..Hence therapies for patients with particular combinations of histone modifications present opportunity to design innovative patient-tailored treatment protocols...
- ATRX tolerates activity-dependent histone H3 methyl/phos switching to maintain repetitive element silencing in neuronsKyung Min Noh
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065
Proc Natl Acad Sci U S A 112:6820-7. 2015..g., histone H3 lysine 9 tri-methylation (H3K9me3)]...
- Histone variant H3.3 provides the heterochromatic H3 lysine 9 tri-methylation mark at telomeresMaheshi Udugama
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Nucleic Acids Res 43:10227-37. 2015..3K9 trimethylation in heterochromatin formation at telomeres. It provides insights into H3.3 function in maintaining integrity of mammalian constitutive heterochromatin, adding to its role in mediating transcription memory in the genome. ..
- Histone H3F3A and HIST1H3B K27M mutations define two subgroups of diffuse intrinsic pontine gliomas with different prognosis and phenotypesDavid Castel
UMR8203 Vectorologie et Thérapeutiques Anticancéreuses, CNRS, Gustave Roussy, Univ Paris Sud, Université Paris Saclay, 94805, Villejuif, France
Acta Neuropathol 130:815-27. 2015..b>Histone H3 genes mutations were assessed by immunochemistry and direct sequencing, whilst global gene expression profiling ..
- Symplastic/pseudoanaplastic giant cell tumor of the boneJudy Sarungbam
Department of Pathology, Memorial Sloan Kettering Cancer Center MSKCC, 1275 York Avenue, New York, NY, 10065, USA
Skeletal Radiol 45:929-35. 2016..Recently, somatic driver mutations of histone H3.3 exclusively in H3F3A have been described in GCTB...
- K27M mutation in H3F3A in ganglioglioma grade I with spontaneous malignant transformation extends the histopathological spectrum of the histone H3 oncogenic pathwayNatacha Joyon
Neuropathologie, Laboratoire Escourolle, Hôpitaux Universitaires Pitié Salpêtrière Charles Foix, AP HP, Paris, France
Neuropathol Appl Neurobiol . 2016..The most frequent genetic alterations in ganglioglioma are BRAF p.V600E mutation (40-60%), gain of chromosomes 7 (23%) and 5 (18%) (1-4). This article is protected by copyright. All rights reserved...
- Pediatric thalamic glioma with H3F3A K27M mutation, which was detected before and after malignant transformation: a case reportKenichi Ishibashi
Department of Neurosurgery, Osaka City General Hospital, 2 13 22 Miyakojima Hondori, Miyakojima ku, Osaka, 534 0021, Japan
Childs Nerv Syst 32:2433-2438. 2016b>Histone H3.3 (H3F3A) mutation in the codon for lysine 27 (K27M) has been found as driver mutations in pediatric glioblastoma and has been suggested to play critical roles in the pathogenesis of thalamic gliomas and diffuse intrinsic ..
- Clinical, Imaging, Histopathological and Molecular Characterization of Anaplastic GangliogliomaMarc Zanello
From the Department of Neurosurgery MZ, ED, Bd, JP, Department of Neuropathology, Sainte Anne Hospital, Paris, France MP, AT E, FC, FA, PV, Department of Biochemistry, Paul Brousse Hospital, Paris, France RS Department of Pediatric Neurosurgery, Necker Enfants Malades Hospital, Paris, France SP, CS R, MZ, Paris Descartes University, Paris, France MZ, MP, AT E, SP, ED, Bd, FC, FA, CS R, MZ, J FM, JP, PV, Department of Medical Biology and Pathology LL, Department of Neurooncology, UK
J Neuropathol Exp Neurol 75:971-980. 2016..K27M H3F3A mutation (17%). AGGs are malignant neoplasms requiring aggressive oncological treatment. In the perspective of targeted therapies, AGGs should be screened for BRAF V600E, hTERT, ATRX, and mutations of histone genes...
- Histone variant H3F3A promotes lung cancer cell migration through intronic regulationSeong Min Park
Specific Organs Cancer Branch, Research Institute, National Cancer Center, Goyang, Gyeonggi 10408, Republic of Korea
Nat Commun 7:12914. 2016Although several somatic single nucleotide variations in histone H3.3 have been investigated as cancer drivers, other types of aberration have not been well studied. Here, we demonstrate that overexpression of H3F3A, encoding H3...
- H3F3 mutation status of giant cell tumors of the bone, chondroblastomas and their mimics: a combined high resolution melting and pyrosequencing approachThibault Kervarrec
Department of Pathology, University Hospital of Tours, avenue de la Republique, Chambray les Tours, France
Mod Pathol . 2017Behjati et al recently described recurrent mutations of H3F3 genes in giant cell tumors of the bone and chondroblastomas...
- The role of histone modifications and telomere alterations in the pathogenesis of diffuse gliomas in adults and childrenJulieann Lee
Division of Neuropathology, Department of Pathology, University of California, San Francisco, 505 Parnassus Ave, Room M 551, Box 0102, San Francisco, CA, 94143, USA
J Neurooncol . 2017..Mutations including K27M and G34R/V that affect critical amino acids within the N-terminal tail of the histone H3 variants, H3.3 and H3...
- H3.5 is a novel hominid-specific histone H3 variant that is specifically expressed in the seminiferous tubules of human testesRaphael Schenk
HELIOS Children s Hospital, HELIOS Klinikum Wuppertal, Research Laboratory, Witten Herdecke University, Germany
Chromosoma 120:275-85. 2011..Six human histone H3 variants are known to date, not counting CenH3 variants: H3.1, H3.2, H3.3 and the testis-specific H3...
- Levels of potential oral cancer salivary mRNA biomarkers in oral cancer patients in remission and oral lichen planus patientsYi Shing Lisa Cheng
Department of Diagnostic Sciences, Texas A and M University Baylor College of Dentistry, 3302 Gaston Ave, Dallas, TX, USA
Clin Oral Investig 18:985-93. 2014....
- Discovery, identification and sequence analysis of RNAs selected for very short or long poly A tail in immature bovine oocytesMaella Gohin
Centre de Recherche en Biologie de la Reproduction, Faculté des sciences de l agriculture et de l alimentation, Departement des Sciences Animales, 2440 Bl Hochelaga, Pavillon INAF, Universite Laval, Quebec, QC, Canada G1V 0A6
Mol Hum Reprod 20:127-38. 2014..Moreover, we identified deadenylated RNAs in bovine oocytes that may be stored for the long-term process of early embryo development and described a conserved motif enriched in the 3'UTR of deadenylated RNAs...
- The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomesRobert Huether
1 Department of Computational Biology, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA 2
Nat Commun 5:3630. 2014..Collectively, our results help to define the landscape of mutations in epigenetic regulatory genes in paediatric cancer and yield a valuable new database for investigating the role of epigenetic dysregulations in cancer. ..
- Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3Yasuhiro Arimura
Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
Sci Rep 4:7115. 2014The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3...
- Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissuesChiara Romani
Angelo Nocivelli Institute of Molecular Medicine, Division of Gynecologic Oncology, University of Brescia, Brescia, Italy
PLoS ONE 9:e113781. 2014..In conclusion, our results recommend the use of PPIA as a single reference gene to be considered for improved reliability of normalization in gene expression studies involving endometrial tumor samples at different tumor degrees. ..
- Contribution of the two genes encoding histone variant h3.3 to viability and fertility in miceMichelle C W Tang
Department of Zoology, The University of Melbourne, Melbourne, Victoria, Australia Genetics Theme, Murdoch Children s Research Institute, Parkville, Victoria, Australia
PLoS Genet 11:e1004964. 2015..Much remains to be learned of H3.3 functions in germ cell development. Histone H3.3 presents a unique genetic paradigm in that two conventional intron-containing genes encode the identical ..
- The activities of four constitutively expressed promoters in single-copy transgenic rice plants for two homozygous generationsSeung Woon Bang
Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Pyeongchang, 232 916, Korea
Planta 241:1529-41. 2015We have characterized four novel constitutive promoters ARP1, H3F3, HSP and H2BF3 that are active in all tissues/stages of transgenic plants and stable over two homozygous generations...
- Diagnostic, prognostic and predictive relevance of molecular markers in gliomasSebastian Brandner
Division of Neuropathology, The National Hospital for Neurology and Neurosurgery, University College London NHS Foundation Trust, London, UK
Neuropathol Appl Neurobiol 41:694-720. 2015..In a tumour-specific topic, the role of mitogen-activated protein kinase pathway mutations in the pathogenesis of pilocytic astrocytomas will be covered. ..
- Biomarker-driven diagnosis of diffuse gliomasChristina L Appin
Department of Pathology and Laboratory Medicine, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA
Mol Aspects Med 45:87-96. 2015..These gliomas often harbor mutations or activating gene rearrangements in BRAF. ..
- Detecting the H3F3A mutant allele found in high-grade pediatric glioma by real-time PCRRay Zhang
Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 1st SW, Rochester, MN, 55905, USA
J Neurooncol 126:27-36. 2016..80% of DIPG cases and 70% of midline glioblastomas contain a mutation at one allele of the H3F3A gene (encoding histone H3 variant H3.3), replacing the lysine 27 with methionine (K27M)...
- Mutation Analysis of H3F3A and H3F3B as a Diagnostic Tool for Giant Cell Tumor of Bone and ChondroblastomaArjen H G Cleven
Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
Am J Surg Pathol 39:1576-83. 2015....
- Diffuse Midline Gliomas with Histone H3-K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic AlterationsDavid A Solomon
Division of Neuropathology, Department of Pathology, University of California, San Francisco, CA
Brain Pathol 26:569-80. 2016Somatic mutations of the H3F3A and HIST1H3B genes encoding the histone H3 variants, H3.3 and H3.1, were recently identified in high-grade gliomas arising in the thalamus, pons and spinal cord of children and young adults...
- Molecular classification of gliomasKenta Masui
Department of Pathology, Tokyo Women s Medical University, Shinjku ku, Tokyo, Japan Ludwig Institute for Cancer Research, University of California San Diego, La Jolla, CA, USA
Handb Clin Neurol 134:97-120. 2016..Finally, we illustrate how advances in molecular diagnostics affect novel strategies of targeted therapy, thereby raising new challenges and identifying new leads for personalized treatment of glioma patients. ..
- Molecular Diagnostic and Prognostic Subtyping of Gliomas in Tunisian PopulationSaoussen Trabelsi
Department of Cytogenetics, Molecular Genetics and Reproductive Biology, Farhat Hached University Hospital, Street Ibn ELJAZZAR, 4000, Sousse, Tunisia
Mol Neurobiol . 2016..Furthermore, by exhibiting a remarkable methylation profile, it emphasised the oncogenic power of G34R mutation connecting gliomagenesis and chromatin regulation...
- [Molecular Genetics as Best Evidence in Glioma Diagnostics]Kenta Masui
Department of Pathology, Tokyo Women s Medical University
Brain Nerve 68:253-61. 2016....
- Identification of citrullinated peptides in the synovial fluid of patients with rheumatoid arthritis using LC-MALDI-TOF/TOFFei Wang
Institute of Clinical Laboratory Science, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002, China
Clin Rheumatol 35:2185-94. 2016..Interestingly, citrullinated histone H3.3 (H3F3A) was found in OA controls, but not in RA groups...
- Recent Advances on the Molecular Pathology of Glial Neoplasms in Children and AdultsFausto J Rodriguez
Division of Neuropathology, Johns Hopkins University School of Medicine, Baltimore, Maryland Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland Electronic address
J Mol Diagn 18:620-34. 2016..Here, we summarize current concepts in molecular testing for glial tumors, including recent findings by large-scale discovery efforts and technologic advances that are affecting routine diagnostic work. ..
- Co-occurrence of Histone H3 K27M and BRAF V600E mutations in paediatric midline grade I gangliogliomaMélanie Pages
Department of Neuropathology, Sainte Anne Hospital, Paris, France
Brain Pathol . 2016..This article is protected by copyright. All rights reserved...
- Bithalamic Gliomas May Be Molecularly Distinct From Their Unilateral High-Grade CounterpartsAlberto Broniscer
Department of Oncology, St Jude Children s Research Hospital, Memphis, TN, USA
Brain Pathol . 2016..This finding suggests that unilateral and bithalamic high-grade gliomas may represent two distinct molecular entities. This article is protected by copyright. All rights reserved...
- Recurrent somatic alterations of FGFR1 and NTRK2 in pilocytic astrocytomaDavid T W Jones
Division of Pediatric Neurooncology, German Cancer Research Center DKFZ, Heidelberg, Germany
Nat Genet 45:927-32. 2013..Our findings thus identify new potential therapeutic targets in distinct subsets of pilocytic astrocytoma and childhood glioblastoma. ..
- Molecular neuro-oncology in clinical practice: a new horizonMichael Weller
Department of Neurology, University Hospital Zurich, Zurich, Switzerland
Lancet Oncol 14:e370-9. 2013..large-scale molecular profiling approaches have identified new mutations in gliomas, affecting IDH1, IDH2, H3F3, ATRX, and CIC, which has allowed subclassification of gliomas into distinct molecular subgroups with ..
- Comprehensive analysis of transcriptome variation uncovers known and novel driver events in T-cell acute lymphoblastic leukemiaZeynep Kalender Atak
Laboratory of Computational Biology, Center for Human Genetics, KU Leuven, Leuven, Belgium
PLoS Genet 9:e1003997. 2013....
- Di- and tri- but not monomethylation on histone H3 lysine 36 marks active transcription of genes involved in flowering time regulation and other processes in Arabidopsis thalianaLin Xu
Institut de Biologie Moléculaire des Plantes CNRS, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
Mol Cell Biol 28:1348-60. 2008..Together our results reveal that the deposition of H3K36 methylation is finely regulated, possibly to cope with the complex regulation of growth and development in higher eukaryotes...
- Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assemblyBin Kang
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
Genes Dev 25:1359-64. 2011b>Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3...
- Recognition of unmodified histone H3 by the first PHD finger of bromodomain-PHD finger protein 2 provides insights into the regulation of histone acetyltransferases monocytic leukemic zinc-finger protein (MOZ) and MOZ-related factor (MORF)Su Qin
Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
J Biol Chem 286:36944-55. 2011..Here we show that the first PHD finger of BRPF2 specifically recognizes the N-terminal tail of unmodified histone H3 (unH3) and report the solution structures of this PHD finger both free and in complex with the unH3 peptide...
- A comparative molecular dynamics study of methylation state specificity of JMJD2AOzlem Ulucan
Center for Computational Biology and Bioinformatics and College of Engineering, Koc University, Istanbul, Turkey
PLoS ONE 6:e24664. 2011..Our study provides new insights into how JMJD2A discriminates between its substrates from both a structural and dynamical point of view...
- Histone chaperone FACT coordinates nucleosome interaction through multiple synergistic binding eventsDuane D Winkler
Howard Hughes Medical Institute and the Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523 1870, USA
J Biol Chem 286:41883-92. 2011..Together, the data reveal that specific FACT subunits synchronize interactions with various target sites on individual nucleosomes to generate a high affinity binding event and promote reorganization...
- CBX3 regulates efficient RNA processing genome-wideAndrea Smallwood
Ludwig Institute for Cancer Research, La Jolla, California 92093, USA
Genome Res 22:1426-36. 2012..In addition, we observed defective recruitment of splicing factors, including SNRNP70, to CBX3 target genes. Collectively, our data suggest a role for CBX3 in aiding in efficient cotranscriptional RNA processing...
- Frequent ATRX mutations and loss of expression in adult diffuse astrocytic tumors carrying IDH1/IDH2 and TP53 mutationsXiao Yang Liu
Department of Human Genetics, McGill University, Montreal, QC, Canada
Acta Neuropathol 124:615-25. 2012..Combined alteration of these genes may contribute to drive the neoplastic growth in a major subset of diffuse astrocytomas in adults...
- CLN5 and CLN8 protein association with ceramide synthase: biochemical and proteomic approachesSaria El Haddad
Department of Pediatric, American University of Beirut, Beirut, Lebanon
Electrophoresis 33:3798-809. 2012..We explore the role of the CLN5/CLN8 proteins in ceramide species specific sphingolipid de novo synthesis, and suggest that CLN5/CLN8 proteins are more closely related than previously believed...
- Mutations in SETD2 and genes affecting histone H3K36 methylation target hemispheric high-grade gliomasAdam M Fontebasso
Division of Experimental Medicine, McGill University and McGill University Health Centre, Montreal, QC, Canada
Acta Neuropathol 125:659-69. 2013Recurrent mutations affecting the histone H3.3 residues Lys27 or indirectly Lys36 are frequent drivers of pediatric high-grade gliomas (over 30% of HGGs)...
- Whole-genome sequencing identifies genetic alterations in pediatric low-grade gliomasJinghui Zhang
Department of Computational Biology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
Nat Genet 45:602-12. 2013..Focusing on the therapeutically challenging diffuse LGGs, our study of 151 tumors has discovered genetic alterations and potential therapeutic targets across the entire range of pediatric LGGs and LGGNTs...
- G34, another connection between MYCN and a pediatric tumorMiller Huang
University of California San Francisco, San Francisco, CA, USA
Cancer Discov 3:484-6. 2013..In this issue of Cancer Discovery, Bjerke and colleagues identify mutations at G34 in H3F3A that result in elevated expression of MYCN as a potential mechanism in gliomagenesis...
- Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAsD Wells
Proc Natl Acad Sci U S A 82:2834-8. 1985..Hybridization of subsegments of the cDNA to human genomic DNA reveals a complex multigene family. The differences in the structures of basal and cell-cycle histone genes suggest a model to explain the differences in their expression...
- Unusual structure, evolutionary conservation of non-coding sequences and numerous pseudogenes characterize the human H3.3 histone multigene familyD Wells
Nucleic Acids Res 15:2871-89. 1987..We also present the sequence of three related processed human H3.3 pseudogenes and provide evidence demonstrating that most of the 20 to 30 copies of the H3.3 gene within the human genome are in fact processed pseudogenes...
- Human spleen histone H3. Isolation and amino acid sequenceY Ohe
J Biochem 90:1205-11. 1981The amino acid sequence of human spleen histone H3 was investigated as a study in histone evolution, following previous investigations of human spleen histone H2B [Ohe, Y., Hayashi, H., & Iwai, K. (1979) J. Biochem...
- The human replacement histone H3.3B gene (H3F3B)W Albig
Institut fur Biochemie und Molekulare Zellbiologie, Georg August Universitat Gottingen, Germany
Genomics 30:264-72. 1995..This localization of the H3.3B gene and its solitary arrangement contrast with the majority of the replication-dependent histone genes, which form a large cluster on chromosome 6 and a second cluster on chromosome 1...
- The Groucho/transducin-like enhancer of split transcriptional repressors interact with the genetically defined amino-terminal silencing domain of histone H3A Palaparti
Center for Neuronal Survival, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
J Biol Chem 272:26604-10. 1997..and far Western blotting studies show further that native Groucho/TLE proteins interact specifically with histone H3 and not with other core histones...
- Localization of the human H3F3A histone gene to 1q41, outside of the normal histone gene clustersX Lin
Department of Biology, University of Houston, Texas 77204, USA
Genomics 46:526-8. 1997
- Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensationH Goto
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
J Biol Chem 274:25543-9. 1999b>Histone H3 (H3) phosphorylation at Ser(10) occurs during mitosis in eukaryotes and was recently shown to play an important role in chromosome condensation in Tetrahymena...
- Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14W S Lo
Molecular Genetics Program, Wistar Institute, Philadelphia, Pennsylvania 19024, USA
Mol Cell 5:917-26. 2000..We examined the relationship between serine 10 phosphorylation and lysine 14 acetylation in histone H3 and have found that, in vitro, several HAT enzymes displayed increased activity on H3 peptides bearing phospho-..
- Methylation of histone H3 lysine 9 creates a binding site for HP1 proteinsM Lachner
Research Institute of Molecular Pathology, The Vienna Biocenter, Vienna, Austria
Nature 410:116-20. 2001..Here we show that mammalian methyltransferases that selectively methylate histone H3 on lysine 9 (Suv39h HMTases) generate a binding site for HP1 proteins--a family of heterochromatic adaptor ..
- MAP kinases mediate UVB-induced phosphorylation of histone H3 at serine 28S Zhong
Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
J Biol Chem 276:12932-7. 2001b>Histone H3 phosphorylation is related closely to chromatin remodeling and chromosome condensation. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines...
- Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tailsY Zhang
Department of Biochemistry and Biophysics, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, North Carolina 27599 7295, USA
Genes Dev 15:2343-60. 2001
- Methylation at arginine 17 of histone H3 is linked to gene activationUta Maria Bauer
Wellcome CRC Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
EMBO Rep 3:39-44. 2002The nuclear hormone receptor co-activator CARM1 has the potential to methylate histone H3 at arginine residues in vitro. The methyltransferase activity of CARM1 is necessary for its co-activator functions in transient transfection assays...
- Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complexPhilip Zegerman
Wellcome Cancer Research UK Institute, Tennis Court Rd, Cambridge, United Kingdom
J Biol Chem 277:11621-4. 2002..proteins, such as GCN5, bind acetyl lysines and the chromodomain protein, HP1, binds methyl lysine 9 of histone H3. Here we show that the previously characterized transcriptional repressor complex NuRD (nucleosome remodeling ..
- Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensationHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi464 8681, Japan
Genes Cells 7:11-7. 2002b>Histone H3 (H3) phosphorylation plays important roles in mitotic chromosome condensation. We reported that H3 phosphorylation occurs at Ser28, as well as at Ser10 during mitosis, at least in mammals...
- Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinaseUte Preuss
Institute of Genetics, University of Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
Nucleic Acids Res 31:878-85. 2003..Interestingly, Dlk/ZIP kinase phosphorylated histone H3 at a novel site, Thr11, rather than Ser10, which is characteristic of mitotic chromosomes...
- MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14Ana Soloaga
MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK
EMBO J 22:2788-97. 2003..by the rapid induction of immediate-early (IE) genes, which occurs concomitantly with the phosphorylation of histone H3 and the high-mobility-group protein HMG-14...
- Development of a competitive reverse-transcription polymerase chain reaction for quantitative measurement of sulfotransferase subfamily 1A enzyme in human hepatoma cellsHannelore Dassow
Institute of Clinical Pharmacology, Faculty of Medicine, University of Leipzig, Leipzig, Germany
Anal Biochem 319:314-7. 2003
- Differential expression of human replacement and cell cycle dependent H3 histone genesDerk Frank
Institut fur Biochemie und Molekulare Zellbiologie, Abteilung Molekularbiologie, Universitat Gottingen, Humboldtallee 23, D 37073 Gottingen, Germany
Gene 312:135-43. 2003..3A gene expression. The possible functional relevance of the differences between the two H3.3 genes in structure and expression is discussed...
- Human PAD4 regulates histone arginine methylation levels via demethyliminationYanming Wang
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
Science 306:279-83. 2004..Moreover, PAD4 activity is linked with the transcriptional regulation of estrogen-responsive genes in MCF-7 cells. These data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones...
- Mechanism of Gene Silencing by DNA MethylationYi Zhang; Fiscal Year: 2005..The proposed studies will provide a thorough characterization of the MeCP1 complex. They will also reveal the potential targets and repression mechanism of the MeCP1 complex. ..
- Structure and Function of CTCF: Mouse Model StudiesGalina Filippova; Fiscal Year: 2007..1 where CTCF maps. The health-relatedness is that the identified mechanisms can be targeted for both cancer prevention and therapeutic intervention. ..
- Role of histone methyltransferases hDOTIL in leukemogenesisYi Zhang; Fiscal Year: 2009..domain, a signature HMTase motif, and their ability to methylate lysine 79 located within the globular domain of histone H3. Toward understanding the biological function of hDOTIL, we set out to identify its functional partners...
- Mechanisms of Polycomb SilencingYi Zhang; Fiscal Year: 2010..5. .Determine the transcriptional effect and understand the mechanism of H3K27 methylation and H2A ubiquitylation using a reconstituted chromatin transcription system. More description in proposal. ..