Genomes and Genes
Gene Symbol: H3
Description: histone cluster 2 H3 family member c
Alias: H3.2, H3/M, H3F2, H3FM, H3FN, histone H3.2, H3 histone family, member M, H3 histone, family 2, histone 2, H3c, histone H3/m, histone cluster 2, H3c
- 14-3-3 proteins recognize a histone code at histone H3 and are required for transcriptional activationStefan Winter
Max F Perutz Laboratories, Vienna Biocenter, Medical University of Vienna, Vienna, Austria
EMBO J 27:88-99. 2008Interphase phosphorylation of S10 at histone H3 is linked to transcriptional activation of a specific subset of mammalian genes like HDAC1. Recently, 14-3-3 proteins have been described as detectors for this phosphorylated histone H3 form...
- Acetylation by histone acetyltransferase CREB-binding protein/p300 of STAT6 is required for transcriptional activation of the 15-lipoxygenase-1 geneP Shankaranarayanan
Eicosanoid Research Division, Department of Gynaecology, University Medical Centre Benjamin Franklin, Free University Berlin, D 12200 Berlin, Germany
J Biol Chem 276:42753-60. 2001..After 9-11 h, when histones become acetylated, STAT6 binding sites may be demasked so that the phosphorylated and acetylated transcription factor can bind to activate gene transcription...
- DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNASteen K T Ooi
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA
Nature 448:714-7. 2007..Peptide interaction assays showed that DNMT3L specifically interacts with the extreme amino terminus of histone H3; this interaction was strongly inhibited by methylation at lysine 4 of histone H3 but was insensitive to ..
- Phosphorylation of histone H3 Thr-45 is linked to apoptosisPaul J Hurd
Wellcome Trust and Cancer Research UK, Gurdon Institute and Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
J Biol Chem 284:16575-83. 2009..Here, we identify threonine 45 of histone H3 (H3T45) as a site of phosphorylation in vivo...
- The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignmentJun Dai
Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 19:472-88. 2005..Thr 3 of histone H3 is highly conserved, but the significance of its phosphorylation is unclear, and the identity of the corresponding ..
- Recognition of multivalent histone states associated with heterochromatin by UHRF1 proteinNataliya Nady
Ontario Cancer Institute, and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 1L7, Canada
J Biol Chem 286:24300-11. 2011..hemimethylated CpG sites, which are specifically recognized through its SRA domain and also interacts with histone H3 trimethylated on lysine 9 (H3K9me3) in an unspecified manner...
- Mitotic histone H3 phosphorylation by vaccinia-related kinase 1 in mammalian cellsTae Hong Kang
Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790 784, Republic of Korea
Mol Cell Biol 27:8533-46. 2007..In mammals, aurora B is believed to be the mitotic histone H3 Ser10 kinase; however, it is not sufficient to phosphorylate H3 Ser10 with aurora B alone...
- Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensationH Goto
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
J Biol Chem 274:25543-9. 1999Histone H3 (H3) phosphorylation at Ser(10) occurs during mitosis in eukaryotes and was recently shown to play an important role in chromosome condensation in Tetrahymena...
- Organismal differences in post-translational modifications in histones H3 and H4Benjamin A Garcia
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA
J Biol Chem 282:7641-55. 2007..Using a combination of mass spectrometric and immunobiochemical approaches, we show that the PTM profile of histone H3 differs significantly among the various model organisms examined...
- Methylation of histone H3 lysine 9 creates a binding site for HP1 proteinsM Lachner
Research Institute of Molecular Pathology, The Vienna Biocenter, Vienna, Austria
Nature 410:116-20. 2001..Here we show that mammalian methyltransferases that selectively methylate histone H3 on lysine 9 (Suv39h HMTases) generate a binding site for HP1 proteins--a family of heterochromatic adaptor molecules ..
- Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damageHengbin Wang
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Kaul Human Genetics Building Room 402A, 720 South 20th Street, 35294, USA
Mol Cell 22:383-94. 2006..Here, we report the purification and functional characterization of a histone H3 and H4 ubiquitin ligase complex, CUL4-DDB-ROC1...
- Activating signal cointegrator 2 belongs to a novel steady-state complex that contains a subset of trithorax group proteinsYoung Hwa Goo
Department of Life Science, Pohang University of Science and Technology, Korea
Mol Cell Biol 23:140-9. 2003..a highly conserved 130- to 140-amino-acid motif termed the SET domain, which was recently implicated in histone H3 lysine-specific methylation activities...
- Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinaseUte Preuss
Institute of Genetics, University of Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
Nucleic Acids Res 31:878-85. 2003..interacting protein (ZIP) kinase, is a nuclear serine/threonine-specific kinase that phosphorylates core histones H3 and H4, and myosine light chain in vitro...
- IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencingFeng Zhu
Science Park Research Division, The University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA
Mol Cell 27:214-27. 2007..Trimethylated histone H3 lysine 9 (H3-K9) was found to associate with the histone trimethyltransferase Suv39h1 and DNA methyltransferase ..
- Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assemblyBin Kang
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
Genes Dev 25:1359-64. 2011Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3...
- Recognition of enhancer element-specific histone methylation by TIP60 in transcriptional activationKwang Won Jeong
Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, USA
Nat Struct Mol Biol 18:1358-65. 2011..BRG1, leading to increased recruitment of histone methyltransferase MLL1 and increased monomethylation of histone H3 at Lys4...
- Nuclear trafficking, histone cleavage and induction of apoptosis by the meningococcal App and MspA autotransportersAhmed S Khairalla
School of Life Sciences, University of Nottingham, Nottingham, UK
Cell Microbiol 17:1008-20. 2015..whereas in vitro clipping assays confirmed the ability of both proteins to proteolytically cleave the core histone H3. Finally, we show that App and MspA induce a dose-dependent increase in DC death via caspase-dependent apoptosis...
- Acetylation of HIV-1 integrase by p300 regulates viral integrationAnna Cereseto
Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology ICGEB, Trieste, Italy
EMBO J 24:3070-81. 2005..This is the first demonstration that HIV-1 IN activity is specifically regulated by post-translational modification...
- ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formationPanagiota Karagianni
Department of Medicine, Harvard Medical School and Massachusetts General Hospital Cancer Center, Building 149, Room 7 213, 13th Street, Charlestown, MA 02129, USA
Mol Cell Biol 28:705-17. 2008Methylation of histone H3 on lysine 9 is critical for diverse biological processes including transcriptional repression, heterochromatin formation, and X inactivation...
- Combinatorial readout of histone H3 modifications specifies localization of ATRX to heterochromatinSebastian Eustermann
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK
Nat Struct Mol Biol 18:777-82. 2011..wild-type ATRX protein targets pericentric and telomeric heterochromatin for deposition of the histone variant H3.3 by means of a largely unknown mechanism...
- Structures of human nucleosomes containing major histone H3 variantsHiroaki Tachiwana
Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo 162 8480, Japan
Acta Crystallogr D Biol Crystallogr 67:578-83. 2011..In the nucleosome, two copies of each core histone, H2A, H2B, H3 and H4, form a histone octamer which wraps 146 base pairs of DNA around itself...
- Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactionsAlexander J Ruthenburg
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA
Cell 145:692-706. 2011..The second PHD finger of human BPTF is known to specifically recognize histone H3 when methylated on lysine 4 (H3K4me2/3)...
- Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenasesWei Xu
State Key Laboratory of Genetic Engineering, School of Life Sciences, Shanghai Medical School, Fudan University, Shanghai 20032, China
Cancer Cell 19:17-30. 2011..Hence, tumor-derived IDH1 and IDH2 mutations reduce α-KG and accumulate an α-KG antagonist, 2-HG, leading to genome-wide histone and DNA methylation alterations...
- Loss of a DNA binding site within the tail of prelamin A contributes to altered heterochromatin anchorage by progerinFrancine Bruston
Unit of Functional and Adaptive Biology BFA affiliated with CNRS, Universite Paris Diderot Paris 7, Paris, France
FEBS Lett 584:2999-3004. 2010..We show that progerin tail has a reduced DNA/chromatin binding capacity and modified trimethylated H3K27 binding pattern, offering a molecular mechanism for heterochromatin alterations related to HGPS...
- Retroviral cyclin enhances cyclin-dependent kinase-8 activityJoel Rovnak
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA
J Virol 86:5742-51. 2012..activity of immune affinity-purified CDK8 in vitro for RNA polymerase II carboxy-terminal domain (CTD) and histone H3 substrates. PP2A also enhances CDK8 kinase activity in vitro for the CTD but not for histone H3...
- RNF4 interacts with both SUMO and nucleosomes to promote the DNA damage responseLynda M Groocock
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA
EMBO Rep 15:601-8. 2014..Furthermore, RNF4 nucleosome-targeting is crucially required for the repair of TRF2-depleted dysfunctional telomeres by 53BP1-mediated non-homologous end joining. ..
- UHRF1 double tudor domain and the adjacent PHD finger act together to recognize K9me3-containing histone H3 tailSi Xie
Department of Biochemistry, The University of Hong Kong, Hong Kong, China
J Mol Biol 415:318-28. 2012..Here, we report the high-resolution crystal structure of UHRF1 PHD finger in complex with N-terminal tail of histone H3. We found that the preceding zinc-Cys4 knuckle is indispensable for the PHD finger of UHRF1 to recognize the first ..
- Mitotic phosphorylation of histone H3 threonine 80Sharra L Hammond
Department of Biochemistry and Molecular Biology University of Texas MD Anderson Cancer Center Houston, TX USA Department of Molecular and Cellular Biology Baylor College of Medicine Houston, Texas USA
Cell Cycle 13:440-52. 2014..Among the proteins that are phosphorylated during mitosis is histone H3, which is heavily phosphorylated on its N-terminal tail...
- Uhrf1-dependent H3K23 ubiquitylation couples maintenance DNA methylation and replicationAtsuya Nishiyama
Department of Cell Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467 8601, Japan
Nature 502:249-53. 2013..Here we show that Uhrf1-dependent histone H3 ubiquitylation has a prerequisite role in the maintenance DNA methylation...
- Expanding cofactor repertoire of protein lysine methyltransferase for substrate labelingKabirul Islam
Molecular Pharmacology and Chemistry Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States
ACS Chem Biol 6:679-84. 2011..This approach is thus suitable for substrate characterization of G9a and expected to further serve as a starting point to evolve other PKMTs to utilize a similar set of cofactors...
- A positive feedback loop involving Haspin and Aurora B promotes CPC accumulation at centromeres in mitosisFangwei Wang
Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Curr Biol 21:1061-9. 2011Haspin phosphorylates histone H3 at Thr3 (H3T3ph) during mitosis [1, 2], providing a chromatin binding site for the chromosomal passenger complex (CPC) at centromeres to regulate chromosome segregation [3-5]...
- Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligaseLi Lan
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 287:12036-49. 2012..The partial eviction of H3 from the nucleosomes is dependent on ubiquitinated H2A Lys-119/Lys-120...
- Identification and characterization of nardilysin as a novel dimethyl H3K4-binding protein involved in transcriptional regulationJing Li
Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China
J Biol Chem 287:10089-98. 2012..Thus, our study has identified a novel H3K4me2-binding protein and revealed a role of NRDc in transcriptional regulation...
- ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndromeShigeki Iwase
Division of Newborn Medicine, Department of Medicine, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA
Nat Struct Mol Biol 18:769-76. 2011..Here we identify ADD(ATRX) as a previously unknown histone H3-binding module, whose binding is promoted by lysine 9 trimethylation (H3K9me3) but inhibited by lysine 4 ..
- CCAAT displacement protein/cut homolog recruits G9a histone lysine methyltransferase to repress transcriptionHitomi Nishio
Department of Pediatrics, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
Proc Natl Acad Sci U S A 101:11257-62. 2004..with CDP/cut, and CDP/cut is directly associated with an increase in the methylation in vivo of Lys-9 in histone H3 within the CDP/cut-regulatory region of the p21(waf1/cdi1) promoter...
- The plasticity of WDR5 peptide-binding cleft enables the binding of the SET1 family of histone methyltransferasesPamela Zhang
Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada
Nucleic Acids Res 40:4237-46. 2012..KMTs), which includes MLL1-5, SET1A and SET1B, catalyzes the methylation of lysine-4 (Lys-4) on histone H3. Recent reports have demonstrated that a three-subunit complex composed of WD-repeat protein-5 (WDR5), ..
- Structural insight into recognition of methylated histone tails by retinoblastoma-binding protein 1Weibin Gong
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
J Biol Chem 287:8531-40. 2012....
- Controlling nuclear JAKs and STATs for specific gene activation by IFNγEzra N Noon-Song
Department of Microbiology and Cell Science, University of Florida, P O Box 110700, Gainesville, FL 32611 0700, USA
Biochem Biophys Res Commun 410:648-53. 2011..that activated Janus kinases pJAK2 and pJAK1 also played a role in gene activation by phosphorylation of histone H3 on tyrosine 41. This study addresses the question of the role of activated JAKs in specific gene activation by IFNγ...
- JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptorKenichi Yamane
Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Cell 125:483-95. 2006..Thus, our work identifies a histone demethylase and links its function to hormone-dependent transcriptional activation...
- The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylationLipeng Wu
Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
Mol Cell 43:132-44. 2011..H2B K34ub by MSL1/2 directly regulates H3 K4 and K79 methylation through trans-tail crosstalk both in vitro and in cells...
- Enzyme kinetic studies of histone demethylases KDM4C and KDM6A: towards understanding selectivity of inhibitors targeting oncogenic histone demethylasesJan B L Kristensen
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark
FEBS Lett 585:1951-6. 2011..This demonstrates that despite very similar active site topologies, selectivity between Jumonji family histone demethylases can be obtained even with small molecule ligands...
- Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylationScott B Rothbart
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Nat Struct Mol Biol 19:1155-60. 2012..Here we show that UHRF1 association with methylated histone H3 Lys9 (H3K9) is required for DNA methylation maintenance...
- Histone demethylation mediated by the nuclear amine oxidase homolog LSD1Yujiang Shi
Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Cell 119:941-53. 2004..LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription...
- Nucleosomal H2B ubiquitylation with purified factorsJaehoon Kim
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
Methods 54:331-8. 2011....
- Biophysical analysis and small-angle X-ray scattering-derived structures of MeCP2-nucleosome complexesChenghua Yang
Department of Biochemistry and Molecular Biology and Howard Hughes Medical Institute, Colorado State University, Fort Collins, CO 80523 1870, USA
Nucleic Acids Res 39:4122-35. 2011..We present ab initio envelope reconstructions of nucleosomes and their complexes with MeCP2 from SAXS data. SAXS studies also revealed unexpected sequence-dependent conformational variability in the nucleosomes themselves...
- Condensin association with histone H2A shapes mitotic chromosomesKenji Tada
Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
Nature 474:477-83. 2011..Our results elucidate the molecular basis for the spatiotemporal regulation of mitotic chromosome architecture, which is crucial for chromosome partitioning...
- Two histone marks establish the inner centromere and chromosome bi-orientationYuya Yamagishi
Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113 0032, Japan
Science 330:239-43. 2010..Here, we show that the phosphorylation of histone H3-threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 (H2A-S121) ..
- Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1Kyohei Arita
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
Proc Natl Acad Sci U S A 109:12950-5. 2012..crystal structure of the linked histone reader modules of UHRF1 in complex with the amino-terminal tail of histone H3. Our structural and biochemical data provide the basis for combinatorial readout of unmodified Arg-2 (H3-R2) and ..
- Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNAYongxin Yu
Molecular Biology Institute and Department of Biological Chemistry, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USA
Mol Cell 46:7-17. 2012..Of the modified sites, histone H3 lysine 56 (H3K56) is unique in that it lies within the globular core domain near the entry-exit sites of the ..
- TdIF2 is a nucleolar protein that promotes rRNA gene promoter activityKotaro Koiwai
Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, Japan
Genes Cells 16:748-64. 2011..In addition, TdIF2 promotes upstream binding factor (UBF) acetylation in vivo. Thus, TdIF2 might promote hrDNAP activity by suppressing Tip60's HAT activity and promoting UBF acetylation...
- Solution structure of human Brg1 bromodomain and its specific binding to acetylated histone tailsWeiqun Shen
School of Life Sciences, and Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230026, People s Republic of China
Biochemistry 46:2100-10. 2007..0 and 3.6 mM, respectively. In this study the dominant substrate was H3-AcK14 (KD approximately 1.2 mM). Mutagenesis analysis reveals several residues important for the binding specificity...
- Biochemical characterization of human SET and MYND domain-containing protein 2 methyltransferaseJiaquan Wu
Oncology Innovative Medicines Unit, AstraZeneca Pharmaceuticals, 35 Gatehouse Drive, Waltham, Massachusetts 02451, USA
Biochemistry 50:6488-97. 2011..Taken together, these results demonstrated the functional importance of distinct domains in the SMYD family of proteins and further advanced our understanding of the catalytic mechanism of this family...
- Alpha-synuclein acts in the nucleus to inhibit histone acetylation and promote neurotoxicityEirene Kontopoulos
Department of Pathology, Brigham and Women s Hospital, Program in Neuroscience, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Hum Mol Genet 15:3012-23. 2006..Alpha-synuclein binds directly to histones, reduces the level of acetylated histone H3 in cultured cells and inhibits acetylation in histone acetyltransferase assays...
- A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chainAdam J L Cook
Institut Curie, Centre de Recherche, Paris F 75248, France
Mol Cell 44:918-27. 2011..that the histone chaperone nuclear autoantigenic sperm protein (NASP) protects a reservoir of soluble histones H3-H4...
- Nuclear epidermal growth factor receptor modulates cellular radio-sensitivity by regulation of chromatin accessKlaus Dittmann
Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, University of Tubingen, Germany
Radiother Oncol 99:317-22. 2011..Nuclear EGFR is involved in cellular stress management and regulation of cellular radio-sensitivity. The aim of this study was to elucidate the molecular mode of nuclear EGFR action...
- Structural and histone binding ability characterizations of human PWWP domainsHong Wu
Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
PLoS ONE 6:e18919. 2011..While Tudor, chromodomain and MBT domains have long been known to bind methylated histones, PWWP was shown to exhibit histone binding ability only until recently...
- And-1 is required for the stability of histone acetyltransferase Gcn5Y Li
Department of Biochemistry and Molecular Biology, The George Washington University Medical School, Washington, DC 20037, USA
Oncogene 31:643-52. 2012..domain-containing protein) has remarkable capability to regulate the stability of Gcn5 proteins and thereby histone H3 acetylation. We find that And-1 forms a complex with both histone H3 and Gcn5...
- 6-alkylsalicylates are selective Tip60 inhibitors and target the acetyl-CoA binding siteMassimo Ghizzoni
Department of Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen A Deusinglaan 1, 9713 AV, Groningen, The Netherlands
Eur J Med Chem 47:337-44. 2012..In addition, these HAT inhibitors effectively inhibited acetyltransferase activity of nuclear extracts on the histone H3 and H4 at micromolar concentrations.
- Crosstalk between leukemia-associated proteins MOZ and MLL regulates HOX gene expression in human cord blood CD34+ cellsJ Paggetti
Inserm UMR 866, Faculty of Medicine, University of Burgundy, Dijon, France
Oncogene 29:5019-31. 2010..We also found that WDR5, an adaptor protein essential for lysine 4 trimethylation of histone H3 (H3K4me3) by MLL, colocalizes and interacts with MOZ...
- The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5Matthieu Lacroix
Institut de Genetique Moleculaire de Montpellier, Universite de Montpellier, Montpellier 34293, France
EMBO Rep 9:452-8. 2008..PRMT5 bound to COPR5 methylates histone H4 (R3) preferentially when compared with histone H3 (R8), suggesting that COPR5 modulates the substrate specificity of nuclear PRMT5-containing complexes, at least ..
- Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1Ho Geun Yoon
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
EMBO J 22:1336-46. 2003..Together, our data reveal the roles of HDAC3 and TBL/TBLR1 and provide evidence for the functional importance of histone interaction in repression mediated by SMRT-N-CoR complexes...
- Histone demethylation by a family of JmjC domain-containing proteinsYu Ichi Tsukada
Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nature 439:811-6. 2006..protein, JHDM1 (JmjC domain-containing histone demethylase 1), that specifically demethylates histone H3 at lysine 36 (H3-K36)...
- Expression patterns and post-translational modifications associated with mammalian histone H3 variantsSandra B Hake
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 281:559-68. 2006..Interest in non-allelic histone variants has been renewed, in part because of recent work on H3 (and other) histone variants. However, only in mammals do three non-centromeric H3 variants (H3.1, H3.2, and H3...
- Modifications of human histone H3 variants during mitosisBenjamin A Garcia
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA
Biochemistry 44:13202-13. 2005Phosphorylation of histone H3 is a hallmark event in mitosis and is associated with chromosome condensation...
- Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alphaHong Seok Choi
Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA
J Biol Chem 280:13545-53. 2005The mitogen-activated protein kinase cascades elicit modification of chromatin proteins such as histone H3 by phosphorylation concomitant with gene activation...
- Functional characterization of a human histone gene cluster duplicationCorey D Braastad
Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655 0002, USA
Gene 342:35-40. 2004..Levels of messenger RNAs for duplicated histone H4 genes are high relative to those for non-duplicated H4 genes. Our data suggest that transcriptionally robust histone H4 genes may have been preferentially duplicated during evolution...
- Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaksYentram Huyen
Wistar Institute, Philadelphia, Pennsylvania 19104 4268, USA
Nature 432:406-11. 2004..In vitro, the 53BP1 tandem tudor domain bound histone H3 methylated on Lys 79 using residues that form the walls of the pocket; these residues were also required for ..
- Ligand-dependent activation of the farnesoid X-receptor directs arginine methylation of histone H3 by CARM1Meenakshisundaram Ananthanarayanan
Department of Pediatrics, Mount Sinai School of Medicine, New York, New York 10029, USA
J Biol Chem 279:54348-57. 2004..This methyltransferase activity is directed specifically to arginine 17 of histone H3. We demonstrate that FXR is directly associated with co-activator-associated arginine methyltransferase 1 (CARM1) ..
- Human PAD4 regulates histone arginine methylation levels via demethyliminationYanming Wang
Department of Genetic Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
Science 306:279-83. 2004..PAD4 targets multiple sites in histones H3 and H4, including those sites methylated by coactivators CARM1 (H3 Arg17) and PRMT1 (H4 Arg3)...
- Phosphorylation of histone H2A inhibits transcription on chromatin templatesYe Zhang
Department of Pathology, Harvard Medical School, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 279:21866-72. 2004..Furthermore, we found that acetylation of histone H3 by the p300 and CREB-binding protein associated factor, PCAF, suppressed the kinase-dependent inhibition of ..
- Structural basis for the methylation site specificity of SET7/9Jean Francois Couture
Department of Biological Chemistry, University of Michigan, 1301 Catherine Road, Ann Arbor, Michigan 48109 0606, USA
Nat Struct Mol Biol 13:140-6. 2006Human SET7/9 is a protein lysine methyltransferase (PKMT) that methylates histone H3, the tumor suppressor p53 and the TBP-associated factor TAF10...
- BRCA1 supports XIST RNA concentration on the inactive X chromosomeShridar Ganesan
The Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
Cell 111:393-405. 2002..These observations suggest that loss of BRCA1 in female cells may lead to Xi perturbation and destabilization of its silenced state...
- Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensationHidemasa Goto
Division of Biochemistry, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya, Aichi464 8681, Japan
Genes Cells 7:11-7. 2002Histone H3 (H3) phosphorylation plays important roles in mitotic chromosome condensation. We reported that H3 phosphorylation occurs at Ser28, as well as at Ser10 during mitosis, at least in mammals...
- Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivatorsN Shikama
Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom
Mol Cell Biol 20:8933-43. 2000..These results argue that NAP is a functionally important component of the p300 coactivator complex and suggest that NAP may serve as a point of integration between transcriptional coactivators and chromatin...
- The genomic organization of the histone clusters on human 6p21.3J Ahn
Yale Child Health Research Center, Department of Pediatrics, Yale University School of Medicine, 464 Congress Avenue, New Haven, Connecticut 06520 8081, USA
Mamm Genome 10:768-70. 1999
- Prothymosin alpha binds histones in vitro and shows activity in nucleosome assembly assayC Díaz-Jullien
Departamento de Bioquimica y Biologia Molecular, Facultad de Biologia, Universidad de Santiago, Spain
Biochim Biophys Acta 1296:219-27. 1996..Pro T alpha and histones in solution) indicated that Pro T alpha has higher affinity for core histones (particularly H3 and H4) than for H1...
- Fine mapping of the human pentraxin gene region on chromosome 1q23M T Walsh
Department of Genetics and Biotechnology Institute, University of Dublin, Trinity College, Dublin, Ireland
Immunogenetics 44:62-9. 1996..In addition, it contains the histone H3F2 and H4F2 genes and the gene for erythroid alpha-spectrin (SPTA1). The gene order is cen...
- Human spleen histone H3. Isolation and amino acid sequenceY Ohe
J Biochem 90:1205-11. 1981The amino acid sequence of human spleen histone H3 was investigated as a study in histone evolution, following previous investigations of human spleen histone H2B [Ohe, Y., Hayashi, H., & Iwai, K. (1979) J. Biochem...
- A major human histone gene cluster on the long arm of chromosome 1L Green
Science 226:838-40. 1984..blot analysis of DNA's from a series of mouse-human somatic cell hybrids with 32P-labeled cloned human H4 and H3 histone DNA as probes...
- Human CpG binding protein interacts with MLL1, MLL2 and hSet1 and regulates Hox gene expressionKhairul I Ansari
Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, Texas 76019, USA
Biochim Biophys Acta 1779:66-73. 2008Human encodes several histone H3-Lysine 4 (H3K4) specific methyl-transferases (HMTs) such as MLL1 (mixed lineage leukemia 1), MLL2, MLL3, hSet1 etc, that play critical roles in gene expression...
- Histone H3-K56 acetylation is important for genomic stability in mammalsJian Yuan
Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, MN 555902, USA
Cell Cycle 8:1747-53. 2009Histone H3 lysine 56 acetylation (H3K56Ac) has recently been identified and shown to be important for genomic stability in yeast. However, whether or not H3K56 acetylation occurs in mammals is not clear...
- HBO1 HAT complexes target chromatin throughout gene coding regions via multiple PHD finger interactions with histone H3 tailNehme Saksouk
Laval University Cancer Research Center, Hôtel Dieu de Québec CHUQ, Quebec City, QC, Canada
Mol Cell 33:257-65. 2009..Biochemical and functional analyses indicate that these domains interact with histone H3 N-terminal tail region, but with a different specificity toward its methylation status...
- Sustained JNK1 activation is associated with altered histone H3 methylations in human liver cancerQingshan Chang
Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
J Hepatol 50:323-33. 2009..It remains unclear whether JNK activation plays an important role in human HCC and, if so, how JNK signaling contributes to the initiation or progression of HCC...
- Histone H3K4me3 binding is required for the DNA repair and apoptotic activities of ING1 tumor suppressorP V Peña
Department of Pharmacology, University of Colorado Health Sciences Center, 12801 East 17th Avenue, Aurora, CO 80045 0511, USA
J Mol Biol 380:303-12. 2008..apoptotic activities of ING1 require the interaction of the C-terminal plant homeodomain (PHD) finger with histone H3 trimethylated at Lys4 (H3K4me3)...
- Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tailsYing Liu
Biochemistry 47:6403-17. 2008..With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K12 and H3-AcK9/K14 peptides. Solution structure of the second bromodomain (BD) is reported in this research...
- Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3Keyna Kobza
Departments of Nutrition and Health Sciences, University of Nebraska at Lincoln, Lincoln, NE, USA
BMB Rep 41:310-5. 2008..Incubation of recombinant BirA ligase with H3-based synthetic peptides showed that lysines 4, 9, 18, and 23 in histone H3 are the targets for the biotinylation by ..
- Atrophin recruits HDAC1/2 and G9a to modify histone H3K9 and to determine cell fatesLei Wang
Department of Physiology and Biophysics, UMDNJ Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA
EMBO Rep 9:555-62. 2008..and that both the ELM2 and SANT domains orchestrate molecular events that lead to a stable methylation of histone H3-lysine 9...
- Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequencesF Marashi
Biochem Cell Biol 64:277-89. 1986We examined the structural and functional properties of a human H3 histone gene promoter. The complete nucleotide sequence of an H3 structural gene and 515 nucleotides of 5' and 100 nucleotides of 3' flanking sequences were determined...
- Molecular basis of histone H3K4me3 recognition by ING4Alicia Palacios
Structural Biology Unit, CIC bioGUNE, Parque Tecnologico de Bizkaia, Edificio 800, 48160 Derio, Spain
J Biol Chem 283:15956-64. 2008..Our results illustrate the versatility of PHD fingers as readers of the histone code...
- A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylationHeinz Neumann
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, England, UK
Mol Cell 36:153-63. 2009..With these designer nucleosomes, we demonstrate that, in contrast to the prevailing dogma, acetylation of H3 K56 does not directly affect the compaction of chromatin and has modest effects on remodeling by SWI/SNF and RSC...
- PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylationDawin Hyllus
Institute of Molecular Biology and Tumor Research IMT, Philipps University of Marburg, 35032 Marburg, Germany
Genes Dev 21:3369-80. 2007..We show here that PRMT6 methylates histone H3 at R2 and histones H4/H2A at R3 in vitro...
- Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulationEric Metzger
Universitäts Frauenklinik und Zentrale Klinische Forschung, Klinikum der Universität Freiburg, Breisacherstrasse 66, 79106 Freiburg, Germany
Nat Cell Biol 10:53-60. 2008..Here we show that protein-kinase-C-related kinase 1 (PRK1) phosphorylates histone H3 at threonine 11 (H3T11) upon ligand-dependent recruitment to androgen receptor target genes...
- Recognition of unmethylated histone H3 lysine 4 links BHC80 to LSD1-mediated gene repressionFei Lan
Department of Pathology, Harvard Medical School, 77 Ave Louis Pasteur, Boston, Massachusetts 02115, USA
Nature 448:718-22. 2007..LSD1 is a lysine-specific histone demethylase that represses transcription by demethylating histone H3 on lysine 4 (ref. 1)...
- PIM1-dependent phosphorylation of histone H3 at serine 10 is required for MYC-dependent transcriptional activation and oncogenic transformationAlessio Zippo
Dipartimento di Biologia Molecolare Università di Siena, Via Fiorentina 1, 53100 Siena, Italy
Nat Cell Biol 9:932-44. 2007..PIM1 to the E boxes of the MYC-target genes FOSL1 (FRA-1) and ID2, and PIM1 phosphorylates serine 10 of histone H3 (H3S10) on the nucleosome at the MYC-binding sites, contributing to their transcriptional activation...
- Identification of histone H3 lysine 36 acetylation as a highly conserved histone modificationStephanie A Morris
Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
J Biol Chem 282:7632-40. 2007..Here we identify and characterize histone H3K36 acetylation (H3K36ac). By mass spectrometric analyses of H3 purified from Tetrahymena thermophila and Saccharomyces cerevisiae (yeast), we find that H3K36 can be acetylated or ..
- Histone acetylation-independent transcription stimulation by a histone chaperoneKohsuke Kato
Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
Nucleic Acids Res 35:705-15. 2007..These results suggest that TAF-I remodel the chromatin structure through its interaction with the core domain of the histones, including the histone fold, and this mechanism is independent of the histone acetylation status...
- Quantitative proteomic analysis of post-translational modifications of human histonesHans Christian Beck
Danish Technological Institute, Holbergsvej 10, DK 6000 Kolding, Denmark
Mol Cell Proteomics 5:1314-25. 2006..A total of 32 acetylations, methylations, and ubiquitinations were located in the human histones H2A, H2B, H3, and H4, including seven novel modifications...
- Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17Feng Miao
Gonda Diabetes Center, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
Mol Endocrinol 20:1562-73. 2006..the coactivators p160 and cAMP response element binding protein-binding protein (CBP) and methylation of histone H3 at arginine 17 (H3-R17)...