GLUT4

Summary

Gene Symbol: GLUT4
Description: solute carrier family 2 member 4
Alias: GLUT4, solute carrier family 2, facilitated glucose transporter member 4, GLUT-4, glucose transporter type 4, insulin-responsive, insulin-responsive glucose transporter type 4, solute carrier family 2 (facilitated glucose transporter), member 4
Species: human

Top Publications

  1. ncbi Evidence for involvement of protein kinase C (PKC)-zeta and noninvolvement of diacylglycerol-sensitive PKCs in insulin-stimulated glucose transport in L6 myotubes
    G Bandyopadhyay
    J. A. Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Endocrinology 138:4721-31. 1997
  2. ncbi Decreased expression of the GLUT4 glucose transporter protein in adipose tissue during pregnancy
    S Okuno
    First Department of Internal Medicine, Nagasaki University School of Medicine, Japan
    Horm Metab Res 27:231-4. 1995
  3. ncbi Insulin regulation of vascular smooth muscle glucose transport in insulin-sensitive and resistant rats
    W J Banz
    Department of Nutrition, University of Tennessee, Knoxville, USA
    Horm Metab Res 28:271-5. 1996
  4. ncbi Impaired regulation of glucose transporter 4 gene expression in insulin resistance associated with in utero undernutrition
    D Jaquet
    INSERM, U 457, Hopital Robert Debre, 75019 Paris, France
    J Clin Endocrinol Metab 86:3266-71. 2001
  5. doi Impact of metformin and rosiglitazone treatment on glucose transporter 4 mRNA expression in women with polycystic ovary syndrome
    Mojca Jensterle
    Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Zaloska 7, SI 1000 Ljubljana, Slovenia
    Eur J Endocrinol 158:793-801. 2008
  6. ncbi Molecular scanning of insulin-responsive glucose transporter (GLUT4) gene in NIDDM subjects
    W H Choi
    Charles A Dana Research Institute, Boston, Massachusetts
    Diabetes 40:1712-8. 1991
  7. ncbi Identification of a novel gene encoding an insulin-responsive glucose transporter protein
    M J Birnbaum
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
    Cell 57:305-15. 1989
  8. ncbi Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver
    E D Abel
    Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 409:729-33. 2001
  9. ncbi Insulin signalling and the regulation of glucose and lipid metabolism
    A R Saltiel
    Life Sciences Institute, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA
    Nature 414:799-806. 2001
  10. ncbi Regulated transport of the glucose transporter GLUT4
    Nia J Bryant
    Garvan Institute of Medical Research, 384 Victoria Road, Darlinghurst, New South Wales 2010, Australia
    Nat Rev Mol Cell Biol 3:267-77. 2002

Research Grants

  1. Effect of exposure to organochlorine compounds on the development of obesity and
    GEORGE E HOWELL; Fiscal Year: 2011
  2. The Role of Myosin in Vesicle Trafficking
    Yashomati M Patel; Fiscal Year: 2010
  3. The role of exposure to bioaccumulative organochlorine compounds on the occurrenc
    GEORGE E HOWELL; Fiscal Year: 2010
  4. Timothy E McGraw; Fiscal Year: 2016
  5. Jingshi Shen; Fiscal Year: 2016
  6. Regulatory Mechanisms of GLUT4 Exocytosis
    Jingshi Shen; Fiscal Year: 2012
  7. REGULATION OF PROTEIN TRAFFICKING IN ADIPOCYTES
    Mike M Mueckler; Fiscal Year: 2013
  8. Barbara B Kahn; Fiscal Year: 2015
  9. Mechanisms of Membrane-Based Insulin Resistance &Therapeutic Reversal Strategies
    Jeffrey S Elmendorf; Fiscal Year: 2012
  10. Insulin Action on GLUT4 and FOXO1 in the Brain
    Garrett Heinrich; Fiscal Year: 2012

Detail Information

Publications384 found, 100 shown here

  1. ncbi Evidence for involvement of protein kinase C (PKC)-zeta and noninvolvement of diacylglycerol-sensitive PKCs in insulin-stimulated glucose transport in L6 myotubes
    G Bandyopadhyay
    J. A. Haley Veterans Hospital and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa 33612, USA
    Endocrinology 138:4721-31. 1997
    ..there was full retention of PKC-zeta, as well as insulin-stimulated glucose transport and translocation of GLUT4 and GLUT1 to the plasma membrane...
  2. ncbi Decreased expression of the GLUT4 glucose transporter protein in adipose tissue during pregnancy
    S Okuno
    First Department of Internal Medicine, Nagasaki University School of Medicine, Japan
    Horm Metab Res 27:231-4. 1995
    ..We therefore investigated the GLUT4 glucose transporter protein in adipose tissues from the pregnant women with normal glucose tolerance and from ..
  3. ncbi Insulin regulation of vascular smooth muscle glucose transport in insulin-sensitive and resistant rats
    W J Banz
    Department of Nutrition, University of Tennessee, Knoxville, USA
    Horm Metab Res 28:271-5. 1996
    ..Accordingly, we measured insulin regulated glucose transport and GLUT4 protein in aortic tissue from insulin-resistant and sensitive rats in order to document classical insulin action ..
  4. ncbi Impaired regulation of glucose transporter 4 gene expression in insulin resistance associated with in utero undernutrition
    D Jaquet
    INSERM, U 457, Hopital Robert Debre, 75019 Paris, France
    J Clin Endocrinol Metab 86:3266-71. 2001
    ..of insulin receptor, insulin receptor substrate-1, p85alpha phosphatidylinositol 3-kinase, glucose transporter-4 (GLUT4), hexokinase II, and glycogen synthase was studied in skeletal muscle at baseline and after a 3-h euglycemic ..
  5. doi Impact of metformin and rosiglitazone treatment on glucose transporter 4 mRNA expression in women with polycystic ovary syndrome
    Mojca Jensterle
    Department of Endocrinology, Diabetes and Metabolic Diseases, University Medical Centre, Zaloska 7, SI 1000 Ljubljana, Slovenia
    Eur J Endocrinol 158:793-801. 2008
    ..state of the polycystic ovary syndrome (PCOS) was found to be associated with a decreased glucose transporter GLUT4 expression in the insulin target tissues...
  6. ncbi Molecular scanning of insulin-responsive glucose transporter (GLUT4) gene in NIDDM subjects
    W H Choi
    Charles A Dana Research Institute, Boston, Massachusetts
    Diabetes 40:1712-8. 1991
    ..the prevalence of mutations in the gene encoding the major insulin-responsive facilitative glucose transporter (GLUT4) in patients with non-insulin-dependent diabetes mellitus (NIDDM)...
  7. ncbi Identification of a novel gene encoding an insulin-responsive glucose transporter protein
    M J Birnbaum
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115
    Cell 57:305-15. 1989
    ....
  8. ncbi Adipose-selective targeting of the GLUT4 gene impairs insulin action in muscle and liver
    E D Abel
    Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 409:729-33. 2001
    ..The glucose transporter GLUT4 mediates insulin-stimulated glucose uptake in adipocytes and muscle by rapidly moving from intracellular storage ..
  9. ncbi Insulin signalling and the regulation of glucose and lipid metabolism
    A R Saltiel
    Life Sciences Institute, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA
    Nature 414:799-806. 2001
    ..But recent studies have shown that numerous other hormones and signalling events attenuate insulin action, and are important in type 2 diabetes...
  10. ncbi Regulated transport of the glucose transporter GLUT4
    Nia J Bryant
    Garvan Institute of Medical Research, 384 Victoria Road, Darlinghurst, New South Wales 2010, Australia
    Nat Rev Mol Cell Biol 3:267-77. 2002
    In muscle and fat cells, insulin stimulates the delivery of the glucose transporter GLUT4 from an intracellular location to the cell surface, where it facilitates the reduction of plasma glucose levels...
  11. ncbi Reduced expression of PGC-1 and insulin-signaling molecules in adipose tissue is associated with insulin resistance
    A Hammarstedt
    The Lundberg Laboratory for Diabetes Research, Department of Internal Medicine, Sahlgrenska University Hospital, SE 413 45 Goteborg, Sweden
    Biochem Biophys Res Commun 301:578-82. 2003
    ..The combined reduction of PGC-1 and insulin signaling molecules in adipose tissue implicates adipose tissue dysfunction which, in turn, can impair the systemic insulin response in the insulin-resistant subjects...
  12. pmc GLUT4 recycles via a trans-Golgi network (TGN) subdomain enriched in Syntaxins 6 and 16 but not TGN38: involvement of an acidic targeting motif
    Annette M Shewan
    Institute for Molecular Biosciences, University of Queensland, St Lucia, Brisbane, QLD 4072, Australia
    Mol Biol Cell 14:973-86. 2003
    Insulin stimulates glucose transport in fat and muscle cells by triggering exocytosis of the glucose transporter GLUT4. To define the intracellular trafficking of GLUT4, we have studied the internalization of an epitope-tagged version of ..
  13. ncbi 5-amino-imidazole carboxamide riboside increases glucose transport and cell-surface GLUT4 content in skeletal muscle from subjects with type 2 diabetes
    Heikki A Koistinen
    Department of Surgical Sciences, Karolinska Hospital, Karolinska Institutet, von Eulers vag 4, II tr, SE 171 77 Stockholm, Sweden
    Diabetes 52:1066-72. 2003
    ..We determined in vitro effects of insulin and/or AICAR exposure on glucose transport and cell-surface GLUT4 content in skeletal muscle from nondiabetic men and men with type 2 diabetes...
  14. pmc Regulation of the human GLUT4 gene promoter: interaction between a transcriptional activator and myocyte enhancer factor 2A
    John B Knight
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Room 853 BMSB, Oklahoma City, OK 73190, USA
    Proc Natl Acad Sci U S A 100:14725-30. 2003
    The GLUT4 gene is subject to complex tissue-specific and metabolic regulation, with a profound impact on insulin-mediated glucose disposal...
  15. ncbi Exercise training increases glycogen synthase activity and GLUT4 expression but not insulin signaling in overweight nondiabetic and type 2 diabetic subjects
    Christine Y Christ-Roberts
    Department of Physiology, University of Texas Health Center at San Antonio, San Antonio, TX 78229, USA
    Metabolism 53:1233-42. 2004
    ..The present study was undertaken to determine how improved insulin signaling, GLUT4 expression, and glycogen synthase activity contribute to this improvement...
  16. ncbi Sortilin is essential and sufficient for the formation of Glut4 storage vesicles in 3T3-L1 adipocytes
    Jun Shi
    Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Dev Cell 9:99-108. 2005
    Impaired translocation of the glucose transporter isoform 4 (Glut4) to the plasma membrane in fat and skeletal muscle cells may represent a primary defect in the development of type 2 diabetes mellitus...
  17. ncbi Full intracellular retention of GLUT4 requires AS160 Rab GTPase activating protein
    Lorena Eguez
    Department of Biochemistry, Weill Cornell Medical School, New York, New York 10021, USA
    Cell Metab 2:263-72. 2005
    Insulin controls glucose flux into muscle and fat by regulating the trafficking of GLUT4 between the interior and surface of cells...
  18. ncbi Lentiviral short hairpin ribonucleic acid-mediated knockdown of GLUT4 in 3T3-L1 adipocytes
    Wei Liao
    Department of Medicine, Division of Endocrinology and Metabolism, University of California, San Diego, La Jolla, 92093, USA
    Endocrinology 147:2245-52. 2006
    ..We chose to target GLUT4 to validate this approach...
  19. doi Development of a novel GLUT4 translocation assay for identifying potential novel therapeutic targets for insulin sensitization
    Franklin Liu
    Metabolic Disorders Diabetes, Merck and Co, Inc, Rahway, NJ 07065, USA
    Biochem J 418:413-20. 2009
    b>GLUT4 (glucose transporter 4) plays important roles in glucose homoeostasis in vivo. GLUT4 expression and function are diminished in diabetic human and animal subjects...
  20. pmc Case-control analysis of SNPs in GLUT4, RBP4 and STRA6: association of SNPs in STRA6 with type 2 diabetes in a South Indian population
    Anup Kumar Nair
    Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, Kerala, India
    PLoS ONE 5:e11444. 2010
    The inverse relationship between GLUT4 and RBP4 expression is known to play a role in the pathogenesis of type 2 diabetes. Elevated levels of RBP4 were shown to cause insulin resistance in muscles and liver...
  21. pmc Insulin-regulated trafficking of GLUT4 requires ubiquitination
    Christopher A Lamb
    Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Traffic 11:1445-54. 2010
    ..of insulin binding its receptor on fat and muscle cells is translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the cell surface where it serves to clear glucose from the bloodstream...
  22. ncbi Insulin receptor and insulin-responsive glucose transporter (GLUT 4) mutations and polymorphisms in a Welsh type 2 (non-insulin-dependent) diabetic population
    S O'Rahilly
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, UK
    Diabetologia 35:486-9. 1992
    We have recently examined the exons encoding the insulin receptor tyrosine kinase domain and GLUT 4 in 30 subjects with Type 2 (non-insulin-dependent) diabetes mellitus using a molecular scanning approach...
  23. pmc The sentrin-conjugating enzyme mUbc9 interacts with GLUT4 and GLUT1 glucose transporters and regulates transporter levels in skeletal muscle cells
    F Giorgino
    Istituto di Clinica Medica, Endocrinologia e Malattie Metaboliche, University of Bari School of Medicine, I 70124 Bari, Italy
    Proc Natl Acad Sci U S A 97:1125-30. 2000
    Glucose transport in insulin-regulated tissues is mediated by the GLUT4 and GLUT1 transporters...
  24. ncbi Enhanced basal activation of mitogen-activated protein kinases in adipocytes from type 2 diabetes: potential role of p38 in the downregulation of GLUT4 expression
    Christian J Carlson
    Insulin Signaling, Metabolic Diseases Division, Global Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, IL 60064, USA
    Diabetes 52:634-41. 2003
    ..phosphorylation status of these MAP kinases was significantly elevated and was associated with decreased IRS-1 and GLUT4 in these fat cells...
  25. ncbi Regulation of GLUT4 expression in vivo and in vitro
    Ann Louise Olson
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Oklahoma City, OK 73190, USA
    Front Biosci 8:s401-9. 2003
    The GLUT4 gene is subject to complex tissue-specific and metabolic regulation that has a profound impact on insulin-mediated glucose disposal...
  26. doi AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5
    Sean L McGee
    Department of Physiology, The University of Melbourne, 3010, Australia
    Diabetes 57:860-7. 2008
    Insulin resistance associated with obesity and diabetes is ameliorated by specific overexpression of GLUT4 in skeletal muscle. The molecular mechanisms regulating skeletal muscle GLUT4 expression remain to be elucidated...
  27. doi Farnesoid X receptor induces GLUT4 expression through FXR response element in the GLUT4 promoter
    Hong Shen
    Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
    Cell Physiol Biochem 22:1-14. 2008
    b>GLUT4, the main insulin-responsive glucose transporter, plays a critical role in maintaining systemic glucose homeostasis and is subject to complicated metabolic regulation...
  28. ncbi Characterization of the molecular mode of action of the sulfonylurea, glimepiride, at adipocytes
    G Muller
    Hoechst AG Frankfurt, Germany
    Horm Metab Res 28:469-87. 1996
    ..potency compared to glibenclamide with respect to stimulation of glucose transport, glucose transporter isoform 4 (GLUT4) translocation and lipid and glycogen synthesis in normal and insulin-resistant adipocytes and in muscle cells, as ..
  29. ncbi Voluntary running improves glucose tolerance and insulin resistance in female spontaneously hypertensive rats
    T L LaPier
    Department of Physical and Occupational Therapy, Idaho State University, Pocatello 83209, USA
    Am J Hypertens 14:708-15. 2001
    ..Hexokinase activity and GLUT4 content were elevated 1.4- and 2...
  30. pmc Activation of protein kinase C zeta induces serine phosphorylation of VAMP2 in the GLUT4 compartment and increases glucose transport in skeletal muscle
    L Braiman
    Faculty of Life Sciences, Gonda-Goldschmied Center, Bar-Ilan University, Ramat-Gan 52900, Israel
    Mol Cell Biol 21:7852-61. 2001
    ..stimulates glucose uptake into skeletal muscle tissue mainly through the translocation of glucose transporter 4 (GLUT4) to the plasma membrane. The precise mechanism involved in this process is presently unknown...
  31. ncbi Inhibition of insulin-induced glucose uptake by atypical protein kinase C isotype-specific interacting protein in 3T3-L1 adipocytes
    K Kotani
    Second Department of Internal Medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan
    J Biol Chem 275:26390-5. 2000
    ..of ASIP inhibited insulin stimulation of both glucose uptake and translocation of the glucose transporter GLUT4 to the plasma membrane, but it did not inhibit glucose uptake induced by either growth hormone or hyperosmolarity ..
  32. ncbi Differential effects of palmitate on glucose uptake in rat-1 fibroblasts and 3T3-L1 adipocytes
    I Usui
    First Department of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan
    Horm Metab Res 31:546-52. 1999
    ..Palmitate treatment did not significantly affect basal and insulin-stimulated GLUT4 translocation...
  33. ncbi Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes
    R Hernandez
    Departamento de Bioquimica y Biologia Molecular II, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
    FEBS Lett 494:225-31. 2001
    ..In consequence, ML-9 precluded insulin stimulation of glucose uptake and GLUT4 translocation to plasma membrane (determined by Western blot), without any effect on the basal glucose uptake...
  34. ncbi The effect of in vivo thyroxine treatment on insulin receptors, glucose transport and GLUT4 in rat adipocytes
    M Fickova
    Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovakia
    Horm Metab Res 29:16-9. 1997
    ..of insulin receptors, decreased insulin stimulatory effect on glucose transport and less glucose transporter (GLUT4) protein in plasma membranes after insulin stimulation as those of controls...
  35. ncbi Expression of GLUT4 glucose transporter protein in adipose tissue and skeletal muscle from streptozotocin-induced diabetic pregnant rats
    K Yamada
    Department of Obstetrics and Gynecology, Mie University School of Medicine, Edobashi, Tsu, Japan
    Horm Metab Res 31:508-13. 1999
    Depletion of GLUT4, the primary glucose transporter protein in adipose tissue and skeletal muscle, is reported to contribute to insulin resistance in pregnancy or diabetes...
  36. ncbi Defective insulin-induced GLUT4 translocation in skeletal muscle of high fat-fed rats is associated with alterations in both Akt/protein kinase B and atypical protein kinase C (zeta/lambda) activities
    F Tremblay
    Department of Physiology, Laval University Hospital Research Center, Quebec, Canada
    Diabetes 50:1901-10. 2001
    ..Insulin-stimulated glucose transport was impaired ( approximately 40-60%) in muscles of high fat-fed rats. Muscle GLUT4 expression was significantly lower in these animals ( approximately 40%, P < 0...
  37. ncbi Insulin activates protein kinases C-zeta and C-lambda by an autophosphorylation-dependent mechanism and stimulates their translocation to GLUT4 vesicles and other membrane fractions in rat adipocytes
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and the Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:25308-16. 1999
    ..C (PKC)-zeta/lambda activity in plasma membranes and microsomes and (b) immunoreactive PKC-zeta and PKC-lambda in GLUT4 vesicles...
  38. ncbi Protein kinase C-zeta as a downstream effector of phosphatidylinositol 3-kinase during insulin stimulation in rat adipocytes. Potential role in glucose transport
    M L Standaert
    J A Haley Veterans Hospital Research Service and Departments of Internal Medicine and Biochemistry Molecular Biology, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 272:30075-82. 1997
    ..Also, in adipocytes transiently expressing hemagglutinin antigen-tagged GLUT4, co-transfection of wild-type or constitutive PKC-zeta stimulated hemagglutinin antigen-GLUT4 translocation, ..
  39. ncbi Biphasic alteration of glucose transport in 3T3-L1 cells during differentiation to the adipocyte-like phenotype
    D Ziehm
    , RWTH Aachen, Germany
    Horm Metab Res 25:71-6. 1993
    Glucose transport activity and subcellular distribution of glucose transporters, GLUT1 and GLUT4 were studied in non-confluent (NCF), confluent (CF), and differentiated 3T3-L1 cells (A)...
  40. ncbi Muscle contractile activity modulates GLUT4 protein content in the absence of insulin
    K Kawanaka
    Division of Health Promotion, National Institute of Health and Nutrition, Shinjuku-ku, Tokyo, Japan
    Horm Metab Res 28:75-80. 1996
    We examined whether muscle contractile activity directly modulates GLUT4 protein content in rat skeletal muscle without the participation of insulin action or via amplified insulin action...
  41. ncbi Okadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 274:14074-8. 1999
    Okadaic acid, an inhibitor of protein phosphatases 1 and 2A, is known to provoke insulin-like effects on GLUT4 translocation and glucose transport, but the underlying mechanism is obscure...
  42. ncbi Direct effects of glimepiride on protein expression of cardiac glucose transporters
    J Eckel
    Laboratory of Molecular Cardiology, Diabetes Research Institute, , Germany
    Horm Metab Res 28:508-11. 1996
    ..on basal and insulin-stimulated glucose uptake and on the expression of the transporter isoforms GLUT1 and GLUT4. Initial rates of 3-O-methylglucose transport in freshly isolated cardiocytes were not acutely affected by ..
  43. ncbi The role of TNF-alpha in human adipose tissue: prevention of weight gain at the expense of insulin resistance?
    F Hube
    German Diabetes Research Institute at the Heinrich Heine University, Dusseldorf
    Horm Metab Res 31:626-31. 1999
    ..receptor (PPAR)-gamma2, lipoprotein lipase (LPL), glycerol-3-phosphate dehydrogenase (GPDH) and GLUT4. Moreover, TNF promotes lipolysis in mature adipocytes and, subsequently, a reversion of the adipocyte phenotype...
  44. ncbi Insulin and PIP3 activate PKC-zeta by mechanisms that are both dependent and independent of phosphorylation of activation loop (T410) and autophosphorylation (T560) sites
    M L Standaert
    J. A. Haley Veterans' Hospital Research Service, and Department of Internal Medicine, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    Biochemistry 40:249-55. 2001
    ..As functional correlates, insulin effects on epitope-tagged GLUT4 translocation were compromised by expression of T410A-PKC-zeta, T560A-PKC-zeta, T410E/T560A, and T410A/T560E-PKC-..
  45. pmc Evidence for defects in the trafficking and translocation of GLUT4 glucose transporters in skeletal muscle as a cause of human insulin resistance
    W T Garvey
    Department of Medicine, Medical University of South Carolina and Ralph H Johnson Veterans Affairs Medical Center, Charleston, South Carolina 29425, USA
    J Clin Invest 101:2377-86. 1998
    ..Since muscle GLUT4 glucose transporter levels are normal in type 2 diabetes, we have tested the hypothesis that insulin resistance is ..
  46. ncbi Caudal hindbrain lactate infusion alters glucokinase, SUR1, and neuronal substrate fuel transporter gene expression in the dorsal vagal complex, lateral hypothalamic area, and ventromedial nucleus hypothalamus of hypoglycemic male rats
    Kamlesh V Vavaiya
    Department of Basic Pharmaceutical Sciences, College of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71209, USA
    Brain Res 1176:62-70. 2007
    ..qPCR was used to measure MCT2, GLUT3, GLUT4, GK, and SUR1 transcripts in the microdissected DVC, VMH, and LHA from groups of male rats treated by continuous ..
  47. ncbi Muscle- and fibre type-specific expression of glucose transporter 4, glycogen synthase and glycogen phosphorylase proteins in human skeletal muscle
    Jens R Daugaard
    Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
    Pflugers Arch 447:452-6. 2004
    The muscle- and fibre type-specific expression of skeletal muscle glucose transporter 4 (GLUT4), glycogen synthase (GS) and glycogen phosphorylase (GP) was investigated in six young male subjects...
  48. ncbi PAX3/forkhead homolog in rhabdomyosarcoma oncoprotein activates glucose transporter 4 gene expression in vivo and in vitro
    Michal Armoni
    Institute of Endocrinology, Diabetes and Metabolism, Rambam Medical Center, Technion Israel Institute of Technology, Haifa 31096, Israel
    J Clin Endocrinol Metab 87:5312-24. 2002
    ..In muscle tissues, glucose transport is primarily mediated by GLUT4. However, the mechanisms that regulate GLUT4 gene expression in tumor tissues are largely unknown...
  49. pmc Stimulation of GLUT4 (glucose transporter isoform 4) storage vesicle formation by sphingolipid depletion
    Zhi jie Cheng
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Biochem J 427:143-50. 2010
    Insulin stimulates glucose transport in fat and skeletal muscle cells primarily by inducing the translocation of GLUT4 (glucose transporter isoform 4) to the PM (plasma membrane) from specialized GSVs (GLUT4 storage vesicles)...
  50. pmc Insulin-induced translocation of the glucose transporter GLUT4 in cardiac muscle: studies on the role of small-molecular-mass GTP-binding proteins
    I Uphues
    Laboratory of Molecular Cardiology, Diabetes Research Institute, Dusseldorf, Germany
    Biochem J 301:177-82. 1994
    ..functional relationship between small-molecular-mass G-proteins and the insulin-responsive glucose transporter GLUT4. Proteins were separated by SDS/PAGE and transferred to nitrocellulose membranes...
  51. pmc Failure of insulin-regulated recruitment of the glucose transporter GLUT4 in cardiac muscle of obese Zucker rats is associated with alterations of small-molecular-mass GTP-binding proteins
    I Uphues
    Laboratory of Molecular Cardiology, Diabetes Research Institute, Dusseldorf, Germany
    Biochem J 311:161-6. 1995
    ..used to study the expression, subcellular distribution and insulin-induced recruitment of the glucose transporter GLUT4 and to elucidate possible molecular alterations of the translocation process...
  52. ncbi Subcellular localization of glucose transporter 4 in the hypothalamic arcuate nucleus of ob/ob mice under basal conditions
    Tadasuke Komori
    Department of Anatomy and Neurobiology, Wakayama Medical University, 811 1 Kimiidera, Wakayama 641 8509, Japan
    Brain Res 1049:34-42. 2005
    ..Although GLUT4 is abundant in the hypothalamus as well as in these peripheral tissues, little is known about the role of GLUT4 in ..
  53. pmc Expression of the glucose transporter GLUT4 in the muscular dystrophic mdx mouse
    C Olichon-Berthe
    INSERM U 145, Faculte de Medecine, Nice, France
    Biochem J 291:257-61. 1993
    ..Western blot analysis with C-terminal-specific anti-peptide antibodies, we investigated the glucose transporters GLUT4 in the different muscle tissues and GLUT1 in brain...
  54. ncbi Effect of vanadate on glucose transporter (GLUT4) intrinsic activity in skeletal muscle plasma membrane giant vesicles
    S Kristiansen
    Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, Denmark
    Biochim Biophys Acta 1282:71-5. 1996
    ..This might be due to vanadate-induced decreased intrinsic activity of GLUT4 accompanying GLUT4 translocation...
  55. ncbi Discrete structural domains determine differential endoplasmic reticulum to Golgi transit times for glucose transporter isoforms
    R C Hresko
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110
    J Biol Chem 269:32110-9. 1994
    The rate of movement of the glucose transporter isoforms Glut1 and Glut4 from the endoplasmic reticulum (ER) to the Golgi apparatus was investigated by pulse labeling and monitoring endoglycosidase H resistance in mRNA-injected Xenopus ..
  56. ncbi Cloning of a pig glucose transporter 4 cDNA fragment: use in developing a sensitive ribonuclease protection assay for quantifying low-abundance glucose transporter 4 mRNA in porcine adipose tissue
    P Y Chiu
    Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802
    J Anim Sci 72:1196-203. 1994
    A 246-bp fragment of porcine glucose transporter 4 (GLUT4) cDNA was cloned by polymerase chain reaction (PCR) from porcine adipose tissue RNA...
  57. doi Chronic ethanol consumption resulting in the downregulation of insulin receptor-beta subunit, insulin receptor substrate-1, and glucose transporter 4 expression in rat cardiac muscles
    Tian Limin
    Department of Endocrinology, Provincial Hospital affiliated to Shandong University, Wenhua Road, Jinan 250021, China
    Alcohol 43:51-8. 2009
    ..of insulin receptor beta subunit (IR-beta), insulin receptor substrate-1 (IRS-1), and glucose transporter 4 (Glut4) in rat cardiac muscle...
  58. ncbi Prenatal expression of the GLUT4 glucose transporter in the mouse
    S J Vannucci
    Department of Pediatrics and Neuroscience and Anatomy, Hershey Medical Center, Penn State University College of Medicine, 17033, USA
    Dev Neurosci 22:274-82. 2000
    The GLUT4 glucose transporter is primarily expressed in skeletal muscle, heart and adipose tissue, where its expression is postnatal, coincident with the acquisition of insulin-regulated glucose transport...
  59. ncbi Insulin regulates the membrane arrival, fusion, and C-terminal unmasking of glucose transporter-4 via distinct phosphoinositides
    Manabu Ishiki
    Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8
    J Biol Chem 280:28792-802. 2005
    Insulin increases glucose uptake into muscle via glucose transporter-4 (GLUT4) translocation to the cell membrane, but the regulated events in GLUT4 traffic are unknown...
  60. pmc Golgin-160 is required for the Golgi membrane sorting of the insulin-responsive glucose transporter GLUT4 in adipocytes
    Dumaine Williams
    Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY 11794, USA
    Mol Biol Cell 17:5346-55. 2006
    ..accumulation of the insulin-responsive amino peptidase (IRAP) and the insulin-regulated glucose transporter (GLUT4) at the plasma membrane concomitant with enhanced glucose uptake in the basal state...
  61. ncbi [Effects of Trimetazidine upon ventricular remodeling and GLUT4 in diabetic rats after myocardial infarction]
    Rui Ying Zhang
    Department of Cardiovascular, First Hospital, Harbin Medical University, Harbin150001, China
    Zhonghua Yi Xue Za Zhi 89:1240-5. 2009
    The aim of study is to investigate the changes of structure and function of heart in diabetic rat after myocardial infarction, and to study the expression of the GLUT4 and the effects of trimetazidine on the ventricular remodeling.
  62. pmc Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular traffic
    Danielle N Gross
    Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    Mol Cell Biol 24:7151-62. 2004
    ..glucose uptake in the NIH-PPAR gamma cells is their virtual lack of the insulin-responsive glucose transporter, Glut4. The NIH-PPAR gamma cells express functionally active components of the insulin receptor-signaling pathway (the ..
  63. ncbi Cell surface labeling of glucose transporter isoform GLUT4 by bis-mannose photolabel. Correlation with stimulation of glucose transport in rat adipose cells by insulin and phorbol ester
    G D Holman
    Department of Biochemistry, University of Bath, United Kingdom
    J Biol Chem 265:18172-9. 1990
    ..We have used this reagent in combination with immunoprecipitation by specific antibodies against the GLUT4 and GLUT1 glucose transporter isoforms to estimate the relative abundance of these two transporters on the surface ..
  64. ncbi Cardiac and adipose tissue abnormalities but not diabetes in mice deficient in GLUT4
    E B Katz
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461 1602, USA
    Nature 377:151-5. 1995
    The insulin-sensitive glucose transporter, GLUT4, is the most abundant facilitative glucose transporter in muscle and adipose tissue, the major sites for postprandial glucose disposal...
  65. ncbi Muscle subcellular localization and recruitment by insulin of glucose transporters and Na+-K+-ATPase subunits in transgenic mice overexpressing the GLUT4 glucose transporter
    T Ramlal
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    Diabetes 45:1516-23. 1996
    Insulin-stimulated glucose uptake in skeletal muscle is mediated through the GLUT4 glucose transporter. Transgenic (TG) mice overexpressing human GLUT4 in skeletal muscle show an increased ability to handle a glucose load...
  66. ncbi Insulin-sensitive glucose transporter transcript levels in calf muscles assessed with a bovine GLUT4 cDNA fragment
    J F Hocquette
    INRA, Laboratoire Croissance et Métabolismes des Herbivores, Theix, France
    Int J Biochem Cell Biol 28:795-806. 1996
    Previous studies have shown that the expression of the insulin-sensitive glucose transporter (GLUT4) is lower in oxidative muscles than in glycolytic muscles in bovines and goats in contrast to observations in rats...
  67. ncbi Molecular cloning and mRNA expression of the bovine insulin-responsive glucose transporter (GLUT4)
    H Abe
    Department of Animal Nutrition, National Institute of Animal Industry, Tsukuba, Japan
    J Anim Sci 75:182-8. 1997
    Insulin-response glucose transporter GLUT4 is a member of the glucose transporter family (GLUT) and is present exclusively in muscle and adipose tissue. It is a target of insulin action in humans and rodents...
  68. pmc Peripheral but not hepatic insulin resistance in mice with one disrupted allele of the glucose transporter type 4 (GLUT4) gene
    L Rossetti
    Department of Medicine, Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Clin Invest 100:1831-9. 1997
    Glucose transporter type 4 (GLUT4) is insulin responsive and is expressed in striated muscle and adipose tissue...
  69. ncbi Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA
    Yoshinori Seki
    Animal Nutrition, Division of Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai 981 8555, Japan
    Gen Comp Endocrinol 133:80-7. 2003
    Identification of insulin-responsive glucose transporter proteins, GLUT4 and GLUT8, was attempted in chickens that characteristically are hyperglycemic and insulin resistant...
  70. ncbi Expression of the insulin-responsive glucose transporter GLUT4 in adipocytes is dependent on liver X receptor alpha
    Knut Tomas Dalen
    Institute for Nutrition Research, University of Oslo, N 0316 Oslo, Norway
    J Biol Chem 278:48283-91. 2003
    The insulin-responsive glucose transporter GLUT4 plays a crucial role in insulin-mediated facilitated glucose uptake into adipose tissue and muscle, and impaired expression of GLUT4 has been linked to obesity and diabetes...
  71. ncbi Calpain system regulates muscle mass and glucose transporter GLUT4 turnover
    Kenichi Otani
    Department of Medicine, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    J Biol Chem 279:20915-20. 2004
    ..Levels of the glucose transporter GLUT4 were increased more than 3-fold in the transgenic mice by Western blotting while mRNA levels for GLUT4 and myocyte ..
  72. ncbi Delayed response of insulin-stimulated fluorine-18 deoxyglucose uptake in glucose transporter-4-null mice hearts
    Marcus V Simoes
    Nuklearmedizinische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universitat Munchen, Munich, Germany
    J Am Coll Cardiol 43:1690-7. 2004
    We sought to evaluate the time course of insulin-stimulated myocardial glucose uptake (MGU) in mice that had undergone ablation of glucose transporter-4 (GLUT4).
  73. ncbi Intracellular delivery of phosphatidylinositol (3,4,5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat cells without increasing glucose uptake
    Gary Sweeney
    Programme in Cell Biology, Hospital for Sick Children, and Department of Biology, York University, Toronto, Ontario, Canada
    J Biol Chem 279:32233-42. 2004
    Insulin stimulates glucose uptake into muscle and fat cells by translocating glucose transporter 4 (GLUT4) to the cell surface, with input from phosphatidylinositol (PI) 3-kinase and its downstream effector Akt/protein kinase B...
  74. ncbi Regulation of insulin-responsive aminopeptidase expression and targeting in the insulin-responsive vesicle compartment of glucose transporter isoform 4-deficient cardiomyocytes
    E Dale Abel
    Division of Endocrinology, Metabolism and Diabetes, Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Mol Endocrinol 18:2491-501. 2004
    In adipocytes and cardiac or skeletal muscle, glucose transporter isoform 4 (GLUT4) is targeted to insulin-responsive intracellular membrane vesicles (IRVs) that contain several membrane proteins, including insulin-responsive ..
  75. ncbi Genetic and nongenetic determinants of skeletal muscle glucose transporter 4 messenger ribonucleic acid levels and insulin action in twins
    Heidi Storgaard
    Steno Diabetes Center, Niels Steensens Vej 2, 2820 Gentofte, Denmark
    J Clin Endocrinol Metab 91:702-8. 2006
    ..uptake in skeletal muscle is mediated through translocation of the insulin-sensitive glucose transporter 4 (GLUT4)-containing vesicles to the plasma membrane...
  76. pmc Identification of amino acid residues within the C terminus of the Glut4 glucose transporter that are essential for insulin-stimulated redistribution to the plasma membrane
    Xiao Mei Song
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 283:12571-85. 2008
    The Glut4 glucose transporter undergoes complex insulin-regulated subcellular trafficking in adipocytes...
  77. pmc Long-term ethanol exposure inhibits glucose transporter 4 expression via an AMPK-dependent pathway in adipocytes
    Li Feng
    Provincial Hospital affiliated to Shandong University, Institute of Endocrinology, Shandong Academy of Clinical Medicine, Ji Nan, China
    Acta Pharmacol Sin 31:329-40. 2010
    ..protein kinase (AMPK) and myocyte enhancer factor 2 isoforms (MEF2A, D) as mediators of the effects of ethanol on glucose transporter 4 (GLUT4) expression are unclear. We studied the effects of ethanol in adipocytes in vivo and in vitro.
  78. doi An inositol 1,4,5-triphosphate (IP3)-IP3 receptor pathway is required for insulin-stimulated glucose transporter 4 translocation and glucose uptake in cardiomyocytes
    A E Contreras-Ferrat
    Centro Estudios Moleculares de la Célula, Facultad de Medicina, and Departamento de Bioquímica y Biología Molecular, Universidad de Chile, Olivos 1007, Santiago 838 0492, Chile
    Endocrinology 151:4665-77. 2010
    ..mechanisms, and their influence on glucose uptake via SLC2 family of facilitative glucose transporter 4 (GLUT4)...
  79. doi Dimethyl sulfoxide enhances GLUT4 translocation through a reduction in GLUT4 endocytosis in insulin-stimulated 3T3-L1 adipocytes
    Marion Berenguer
    INSERM U895, Mediterranean Research Center for Molecular Medicine C3M, Nice, France
    Biochimie 93:697-709. 2011
    Insulin increases muscle and fat cell glucose uptake by inducing the translocation of glucose transporter GLUT4 from intracellular compartments to the plasma membrane...
  80. pmc Glycemic improvement in diabetic db/db mice by overexpression of the human insulin-regulatable glucose transporter (GLUT4)
    E M Gibbs
    Department of Metabolic Diseases, Pfizer, Inc, Groton, Connecticut 06340, USA
    J Clin Invest 95:1512-8. 1995
    The effects of increased GLUT4 (insulin-regulatable muscle/fat glucose transporter) expression on glucose homeostasis in a genetic model of non-insulin-dependent diabetes mellitus were determined by expressing a human GLUT4 transgene (..
  81. ncbi Control of exercise-stimulated muscle glucose uptake by GLUT4 is dependent on glucose phosphorylation capacity in the conscious mouse
    Patrick T Fueger
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 279:50956-61. 2004
    Previous work suggests that normal GLUT4 content is sufficient for increases in muscle glucose uptake (MGU) during exercise because GLUT4 overexpression does not increase exercise-stimulated MGU...
  82. ncbi The Krüppel-like factor KLF15 regulates the insulin-sensitive glucose transporter GLUT4
    Susan Gray
    Cardiovascular Division, Brigham and Women s Hospital, Thorn Building, 20 Shattuck Street, Boston, MA 02115, USA
    J Biol Chem 277:34322-8. 2002
    ..Recent studies suggest that insulin resistance is primarily caused by a defect in glucose transport. GLUT4 is the main insulin-responsive glucose transporter and is expressed predominantly in muscle and adipose tissues...
  83. pmc Regulation of multisite phosphorylation and 14-3-3 binding of AS160 in response to IGF-1, EGF, PMA and AICAR
    Kathryn M Geraghty
    MRC Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 407:231-41. 2007
    AS160 (Akt substrate of 160 kDa) mediates insulin-stimulated GLUT4 (glucose transporter 4) translocation, but is widely expressed in insulin-insensitive tissues lacking GLUT4...
  84. ncbi GLUT4 overexpression in db/db mice dose-dependently ameliorates diabetes but is not a lifelong cure
    J T Brozinick
    Experimental Diabetes, Metabolism and Nutrition Section, DB NIDDK, National Institutes of Health, Bethesda, Maryland, USA
    Diabetes 50:593-600. 2001
    We previously reported that overexpression of GLUT4 in lean, nondiabetic C57BL/KsJ-lepr(db/+) (db/+) mice resulted in improved glucose tolerance associated with increased basal and insulin-stimulated glucose transport in isolated ..
  85. ncbi Acyl-coenzyme A dehydrogenases are localized on GLUT4-containing vesicles via association with insulin-regulated aminopeptidase in a manner dependent on its dileucine motif
    Hideki Katagiri
    Division of Molecular Metabolism and Diabetes, Department of Internal Medicine, Tohoku University Graduate School of Medicine, Sendai 980 8574, Japan
    Mol Endocrinol 16:1049-59. 2002
    Insulin-regulated aminopeptidase (IRAP, also termed vp165) is known to be localized on the GLUT4-containing vesicles and to be recruited to the plasma membrane after stimulation with insulin...
  86. pmc Phosphatidylinositol 4-kinase, but not phosphatidylinositol 3-kinase, is present in GLUT4-containing vesicles isolated from rat skeletal muscle
    S Kristiansen
    Copenhagen Muscle Research Centre, August Krogh Institute, 13 Universitetsparken, DK 2100 Copenhagen, Denmark
    Biochem J 335:351-6. 1998
    ..stimulates the rate of glucose transport into muscle and adipose cells by translocation of glucose transporter (GLUT4)-containing vesicles from an intracellular storage pool to the surface membrane...
  87. pmc In vitro analysis of the glucose-transport system in GLUT4-null skeletal muscle
    J W Ryder
    Department of Clinical Physiology, Karolinska Hospital, S 171 76, Stockholm, Sweden
    Biochem J 342:321-8. 1999
    We have characterized the glucose-transport system in soleus muscle from female GLUT4-null mice to determine whether GLUT1, 3 or 5 account for insulin-stimulated glucose-transport activity. Insulin increased 2-deoxyglucose uptake 2...
  88. ncbi Effects of caudal fourth ventricular lactate infusion on hypoglycemia-associated MCT2, GLUT3, GLUT4, GCK, and sulfonylurea receptor-1 gene expression in the ovariectomized female rat LHA and VMH: impact of estradiol
    Kamlesh V Vavaiya
    Department of Basic Pharmaceutical Sciences, College of Pharmacy, The University of Louisiana at Monroe, Monroe, LA 71209, USA
    J Mol Neurosci 34:121-9. 2008
    ..Neuronal glucose (GLUT3, GLUT4) and monocarboxylate (MCT2) transporter genes are expressed in both sites, suggesting that glucose and lactate, a ..
  89. doi Glucosamine-induced endoplasmic reticulum stress affects GLUT4 expression via activating transcription factor 6 in rat and human skeletal muscle cells
    G A Raciti
    Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita di Napoli Federico II, Via Sergio Pansini, 5, Naples 80131, Italy
    Diabetologia 53:955-65. 2010
    ..Here we sought to evaluate the possible role of endoplasmic reticulum (ER) stress in the induction of insulin resistance by glucosamine in skeletal muscle cells...
  90. pmc GLUT4 trafficking in insulin-stimulated rat adipose cells: evidence that heterotrimeric GTP-binding proteins regulate the fusion of docked GLUT4-containing vesicles
    C M Ferrara
    Experimental Diabetes, Metabolism, and Nutrition Section, Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1420, USA
    Biochem J 343:571-7. 1999
    ..isoproterenol)] decrease, steady-state insulin-stimulated glucose transport activity and cell-surface GLUT4 in isolated rat adipose cells without changing plasma membrane GLUT4 content...
  91. ncbi The adipocyte plasma membrane caveolin functional/structural organization is necessary for the efficient endocytosis of GLUT4
    Satoshi Shigematsu
    Department of Physiology and Biophysics, The University of Iowa, Iowa City 52242, USA
    J Biol Chem 278:10683-90. 2003
    ..well established that insulin stimulation of glucose uptake requires the translocation of intracellular localized GLUT4 protein to the cell surface membrane...
  92. ncbi The insulin-regulated aminopeptidase: a companion and regulator of GLUT4
    Susanna R Keller
    University of Virginia, Department of Internal Medicine Division of Endocrinology, PO Box 801409, Charlottesville VA 22908, USA
    Front Biosci 8:s410-20. 2003
    ..cells as a major protein in intracellular vesicles that also harbor the insulin-responsive glucose transporter GLUT4. IRAP, like GLUT4, predominantly localizes to these intracellular vesicles under basal conditions...
  93. ncbi Association of N-ethylmaleimide sensitive fusion (NSF) protein and soluble NSF attachment proteins-alpha and -gamma with glucose transporter-4-containing vesicles in primary rat adipocytes
    C C Mastick
    Department of Cell Biology, Parke Davis Pharmaceutical Research Division, Warner Lambert Company, Ann Arbor, Michigan 48105, USA
    Endocrinology 138:2391-7. 1997
    ..protein (NSF) and soluble NSF attachment proteins (SNAP)-containing fusion complexes in glucose transporter-4 (GLUT4) membrane trafficking, the subcellular distributions of NSF, alpha-SNAP, and gamma-SNAP in primary rat adipocytes ..
  94. ncbi Glucose transporter levels in a male spontaneous non-insulin-dependent diabetes mellitus rat of the Otsuka Long-Evans Tokushima Fatty strain
    K Toide
    Second Tokushima Institute of New Drug Research, Otsuka Pharmaceutical Co, Ltd, Japan
    Diabetes Res Clin Pract 38:151-60. 1997
    ..GLUT) in the development of diabetes in this model, we examined the action of insulin on the translocation of GLUT4 and GLUT1 in isolated adipocytes, and the GLUT4 protein levels in muscles...
  95. ncbi The antioxidant tempol inhibits cardiac hypertrophy in the insulin-resistant GLUT4-deficient mouse in vivo
    R H Ritchie
    Molecular Pharmacology Laboratory, Wynn Department of Metabolic Cardiology, Baker Heart Research Institute, St Kilda Road Central, Melbourne, VIC 8008, Australia
    J Mol Cell Cardiol 42:1119-28. 2007
    ..Mice with cardiac GLUT4 deletion (GLUT4-knockout), superimposed on global GLUT4 suppression (GLUT4-knockdown) were administered tempol for ..
  96. ncbi Insulin, but not contraction, activates Akt/PKB in isolated rat skeletal muscle
    J T Brozinick
    Howard Hughes Medical Institute, Department of Medicine and The Cox Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    J Biol Chem 273:14679-82. 1998
    ..that is independent of insulin, but the two pathways may converge downstream at the level of stimulation of GLUT4 translocation...
  97. ncbi Igf1 promotes longitudinal bone growth by insulin-like actions augmenting chondrocyte hypertrophy
    J Wang
    Developmental Endocrinology Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    FASEB J 13:1985-90. 1999
    ..The expression of the insulin-sensitive glucose transporter, GLUT4, is significantly decreased and the insulin-regulated enzyme glycogen synthase kinase 3beta (GSK3) is hypo-..
  98. ncbi Molecular aspects of lipoic acid in the prevention of diabetes complications
    L Packer
    Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90098 9121, USA
    Nutrition 17:888-95. 2001
    ..To develop a better understanding of the preventative and therapeutic potentials of LA, much of the current interest is focused on elucidating its molecular mechanisms in redox dependent gene expression...
  99. ncbi Indinavir uncovers different contributions of GLUT4 and GLUT1 towards glucose uptake in muscle and fat cells and tissues
    A Rudich
    Programme in Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, ON, Canada
    Diabetologia 46:649-58. 2003
    Insulin-dependent glucose influx in skeletal muscle and adipocytes is believed to rely largely on GLUT4, but this has not been confirmed directly...
  100. ncbi Enhanced insulin-stimulated glycogen synthesis in response to insulin, metformin or rosiglitazone is associated with increased mRNA expression of GLUT4 and peroxisomal proliferator activator receptor gamma co-activator 1
    L Al-Khalili
    Department of Surgical Science, Karolinska Institute, Stockholm, Sweden
    Diabetologia 48:1173-9. 2005
    ..The aim of this study was to determine the effect of several antidiabetic agents on insulin-stimulated glycogen synthesis, as well as on mRNA expression...
  101. ncbi GLUT4 facilitative glucose transporter specifically and differentially contributes to agonist-induced vascular reactivity in mouse aorta
    James L Park
    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Arterioscler Thromb Vasc Biol 25:1596-602. 2005
    We hypothesized that GLUT4 is a predominant facilitative glucose transporter in vascular smooth muscle cells (VSMCs), and GLUT4 is necessary for agonist-induced VSMC contraction.

Research Grants68

  1. Effect of exposure to organochlorine compounds on the development of obesity and
    GEORGE E HOWELL; Fiscal Year: 2011
    ..preliminary data indicate that exposure to oxychlordane or DDE decreases insulin stimulated glucose transporter 4 (Glut4) translocation in rat skeletal muscle cells...
  2. The Role of Myosin in Vesicle Trafficking
    Yashomati M Patel; Fiscal Year: 2010
    ..Insulin mediates the translocation and fusion of insulin-responsive glucose transporter (GLUT4)-containing vesicles to the plasma membrane;this process is critical for proper glucose homeostasis...
  3. The role of exposure to bioaccumulative organochlorine compounds on the occurrenc
    GEORGE E HOWELL; Fiscal Year: 2010
    ..If so, the present study will provide mechanisms through which these compounds promote this disease and will establish exposure to certain organochlorine compounds as a risk factor for the development of type 2 diabetes. ..
  4. Timothy E McGraw; Fiscal Year: 2016
    ..Insulin regulates glucose flux into fat and muscle cells by controlling the amount of the Glut4 glucose transporter in the plasma membrane...
  5. Jingshi Shen; Fiscal Year: 2016
    ..is to promote glucose uptake into muscle and adipose tissues, a process mediated by the glucose transporter GLUT4. Upon insulin stimulation, GLUT4 is relocated from intracellular storage vesicles to the cell surface through ..
  6. Regulatory Mechanisms of GLUT4 Exocytosis
    Jingshi Shen; Fiscal Year: 2012
    ..is to promote glucose uptake into muscle and adipose tissues, a process mediated by the glucose transporter GLUT4. Upon insulin stimulation, GLUT4 is relocated from intracellular storage vesicles to the cell surface through ..
  7. REGULATION OF PROTEIN TRAFFICKING IN ADIPOCYTES
    Mike M Mueckler; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The Glut4 glucose transporter catalyzes the rate-limiting step in postprandial whole body glucose disposal...
  8. Barbara B Kahn; Fiscal Year: 2015
    ..We showed that the down regulation of Glut4 in adipocytes that occurs in obesity and type 2 diabetes plays an important role in the insulin resistance in ..
  9. Mechanisms of Membrane-Based Insulin Resistance &Therapeutic Reversal Strategies
    Jeffrey S Elmendorf; Fiscal Year: 2012
    ..are an important, unappreciated derangement in insulin resistance contributing to impaired glucose transporter GLUT4 regulation by insulin...
  10. Insulin Action on GLUT4 and FOXO1 in the Brain
    Garrett Heinrich; Fiscal Year: 2012
    ..Insulin promotes glucose uptake through translocation of the facilitative glucose transporter 4 (GLUT4)...
  11. Functional mapping of SNARE-dependent trafficking and fusion
    Jeffrey E Pessin; Fiscal Year: 2013
    ..trafficking, insulin-stimulated translocation and fusion of the insulin-responsive glucose transporter (GLUT4) from intracellular storage sites to the plasma membrane in adipocytes...
  12. CHC22 CLATHRIN FUNCTION IN HUMAN GLUCOSE METABOLISM
    FRANCES BRODSKY; Fiscal Year: 2009
    ..Recent work from the laboratory indicates that CHC22 is involved in intracellular sequestration of the GLUT4 glucose transporter...
  13. Role of TUG in the Formation of Insulin-Responsive GLUT4 Vesicles
    Charisse Orme; Fiscal Year: 2011
    In type 2 diabetes, impaired insulin signaling leads to reduced GLUT4 trafficking and thus decreased glucose uptake in fat and muscle...
  14. The Role of the Rab GAP AS 160 in adipocyte Metabolism
    MELISSA N LANSEY; Fiscal Year: 2010
    ..substrate and Rab GTPase- acfivafing protein (GAP) AS160 in regulafing the trafficking of the glucose transporter GLUT4 and in thus controlling glucose uptake in primary adipocytes...
  15. Investigating the Mechanism by which Glut4 Storage Vesicle (GSV) Fusion is Regula
    MATTHEW MOLSKI; Fiscal Year: 2013
    ..Healthy muscle and adipose cells maintain correct glucose homeostasis by trafficking the Glucose transporter 4 (Glut4) to the plasma membrane in response to insulin stimulation...
  16. The Role of RAB10 in Adipocyte Glucose Transport
    LEIGH AMANDA SADACCA; Fiscal Year: 2013
    ..glucose transporters;in adipocytes and muscle cells, the primary transporter is the glucose transporter 4 (GLUT4)...
  17. Walter J Koch; Fiscal Year: 2016
    ..Specifically, GRK2 has been shown to inhibit the translocation of the glucose transporter, GLUT4, to cell membranes with consequential insulin resistance...
  18. Sharona E Gordon; Fiscal Year: 2016
    ..One of the best-studied examples, trafficking of the Glut4 glucose transporter to the plasma membrane of adipocytes and muscle cells in response to insulin, has revealed a ..
  19. Timothy E McGraw; Fiscal Year: 2016
    ..studies are based on my lab's expertise, in large part developed through studies of the insulin-regulated GLUT4 glucose transport, in the studies of regulated membrane protein trafficking...
  20. ZHEN YUE JIANG; Fiscal Year: 2016
    ..is known that activation of the PI 3-kinase - Akt2 pathway is required for insulin-stimulated glucose transporter GLUT4 translocation from intracellular storage to the plasma membrane (PM) although the molecular mechanism is not fully ..
  21. Susanna R Keller; Fiscal Year: 2014
    ..the maintenance of glucose homeostasis is the control of the subcellular distribution of the glucose transporter GLUT4 in muscle and fat cells...
  22. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  23. Adipose Tissue Branched-Chain Amino Acid Metabolism and Glucose Homeostasis
    Mark A Herman; Fiscal Year: 2010
    In insulin resistant states such as obesity and type 2 diabetes, expression of the insulin-responsive Glut4 glucose transporter is down-regulated in adipose tissue, but not in muscle...
  24. Gene and environment interaction and insulin resistance
    Raquel Villegas; Fiscal Year: 2013
    ..the insulin receptor, INSR, and insulin receptor substrates 1 and 2, IRS1 and IRS2), glucose transporter 4 (GLUT4), and genes identified from GWAS of T2D quantitative traits with insulin resistance in the context of gene-..
  25. INSULIN ACTION IN MUSCLE AND FAT CELLS
    JAMES CARLTON GARRISON; Fiscal Year: 2010
    ..Glucose transport and oxidation, lipid and glycogen synthesis, GLUT4 translocation, and several parameters of insulin signaling will be measured after increasing or decreasing levels ..
  26. SUBCELLULAR LOCALIZATION OF PI 3-KINASE SIGNALING
    Michael P Czech; Fiscal Year: 2011
    ..We focus on insulin-mediated GLUT4 glucose transporter translocation to the plasma membrane of adipocytes as our model system, a seminal problem in ..
  27. High content screening assay for activators of glucose transporter GLUT4
    ZHEN YUE JIANG; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Insulin stimulates glucose disposal through enhancing glucose transporter GLUT4 translocation from intracellular storage to the cell surface where GLUT4 moves glucose into the cells for metabolism...
  28. TUG-mediated GLUT4 glucose transporter trafficking and regulation by post-transla
    JONATHAN PHILIP BELMAN; Fiscal Year: 2012
    ..Glucose transporter 4 (GLUT4) is the major insulin responsive glucose transporter...
  29. The role of proteing TUG in glucose homeostasis
    WHITNEY NICOLE HARRIS; Fiscal Year: 2011
    TUG, Tether containing and UBX domain for GLUT4, is a novel protein that is necessary to sequester GLUT4 away from the plasma membrane during basal states...
  30. Gregory D Cartee; Fiscal Year: 2016
    ..in old rats the effects of CR and exercise, alone and in combination, on insulin-stimulated glucose transport and GLUT4 translocation in skeletal muscle;2) Identify in old rats the specific insulin signaling events in skeletal muscle ..
  31. ARF regulators in endocytic transport
    Victor W Hsu; Fiscal Year: 2013
    ..have identified Grp1 as the GEF that acts on ARF6 for the endocytic recycling of the glucose transporter type 4 (glut4), a process that is regulated by insulin...
  32. Thomas L Clemens; Fiscal Year: 2016
    ..a hyperinsulinemic-euglycemic clamp on circulating undercarboxylated OC levels in both wild-type and mice lacking Glut4 in osteoblasts...
  33. Frances M Brodsky; Fiscal Year: 2016
    ..that CHC22 participates uniquely in retrograde transport from endosomes to the TGN and thereby targets the GLUT4 glucose transporter to the insulin-responsive GLUT4 storage compartment (GSC) in human muscle and fat cells...
  34. GLUT8 Overexpression in Cardioprotection
    Maureen J Charron; Fiscal Year: 2010
    ..We have shown that mice without the insulin responsive glucose transporter, GLUT4 (G4 Null), have hypertrophied hearts that unexpectedly take up more than normal amounts of glucose and exhibit ..
  35. NR4A Orphan Receptors And Insulin Resistance
    W Timothy Garvey; Fiscal Year: 2012
    ..studies will address our preliminary data indicating that NR4A3 modulates insulin-stimulated glucose transport, GLUT4 translocation, and insulin-mediated phosphorylation of signaling molecules...
  36. Molecular Genetics of Regulated Protein Delivery of the Plasma Membrane
    CHRIS ALAN KAISER; Fiscal Year: 2010
    ..For example, the GLUT4 glucose transporter is delivered to the plasma membrane of fat and muscle cells in response to insulin, and ..
  37. INSULIN RECEPTORS AND THE GLUCOSE TRANSPORT SYSTEM
    JERROLD MICHAEL OLEFSKY; Fiscal Year: 2010
    ..are to elucidate the molecular and biochemical events in insulin'sintracellularsignalingpathway leading to GLUT4 translocation and glucose transport and to explore new mechanisms of insulin resistance...
  38. Exercise Regulation of Glucose Metabolism in Skeletal Muscle
    Laurie J Goodyear; Fiscal Year: 2012
    ..We will also determine how AMPK regulates the temporal and spatial dynamics of GLUT4 translocation in the before and after exercise using a novel imaging method that has not previously been used in ..
  39. Impact of Inflammation on the Control of Muscle Glucose Uptake
    Owen P McGuinness; Fiscal Year: 2013
    ..Tissues will be analyzed for glycogen synthesis, insulin signaling, oxidative stress and GLUT4 translocation. Our specific aims are to determine: 1...
  40. Debbie C Thurmond; Fiscal Year: 2016
    ..will be tested in three specific aims: 1) Determine the requirement for Munc18c-Doc2b interaction in regulating GLUT4 translocation and whole body glucose homeostasis;2) Delineate the role of Munc18-Doc2b protein complexes in ..
  41. The function and regulation of TBC1D1 in skeletal muscle metabolism.
    MELISSA ANNE CHAMBERS; Fiscal Year: 2011
    ..TBC1D1 has been identified as an obesity candidate gene in humans that potentially regulates GLUT4 translocation in adipocytes...
  42. ACTIVATION OF HEXOSE TRANSPORT BY INSULIN
    Paul F Pilch; Fiscal Year: 2012
    ..of a vesicular pool of glucose transporters from an intracellular storage site to the cell surface, and it is the Glut4 transporter isoform that functions in this regard...
  43. Engineering an in vitro model of adipose tissue formation and metabolism
    Kyongbum Lee; Fiscal Year: 2010
    ..The initial knockdown targets are: glucose transporter GLUT4;lipogenic enzymes ACC, FAS and AWAT;and lipolysis enzyme lipase...
  44. MOLECULAR BASIS OF INSULIN RESISTANCE
    Maureen J Charron; Fiscal Year: 2010
    ..Human and rodent studies provide strong support for a genetic predisposition to these pathologies. The GLUT4+/- mouse model (G4+/-) represents an example of an `At Risk'individual as male G4+/- mice develop insulin ..
  45. Cytoskeletal Regulation of Erythrocyte Glucose Transporter-1
    Athar H Chishti; Fiscal Year: 2012
    ..We will also investigate the binding of dematin and adducin with GLUT4, and investigate their functional effects on GLUT4 recycling and glucose uptake in adipocytes...
  46. Domenico Accili; Fiscal Year: 2016
    ..The PI presents data putatively identifying an "insulin-sensitive" neuron population, characterized by Glut4 expression;as well as a novel gut epithelial progenitor cell with the unique ability to give rise to bona fide ..
  47. Johnathan D Tune; Fiscal Year: 2016
    ..g. cAMP, PKA, p38 MAPK) and the regulation of myocardial glucose and fatty acid transporters (e.g. GLUT4, FAT/CD36) in lean vs. obese/MetS swine...
  48. Lakshman Segar; Fiscal Year: 2015
    ..Specific Aim 2 will determine the mechanism by which systemic insulin resistance and/or GLUT4 deficiency alter injury-induced neointimal growth and PDGF- vs insulin-induced glucose transport...
  49. Jheem D Medh; Fiscal Year: 2014
    ..of LPL results in a change in the expression pattern of various genes involved in insulin signaling including Glut4, PI3Kinase, acetyl coA carboxylase, etc...
  50. PI-3 kinase effectors in insulin-responsive systems
    Silvia Corvera; Fiscal Year: 2010
    ..glucose uptake in adipocytes, by testing whether insulin activation of Akt2 is impaired, or whether GLUT4 trafficking is impaired in the absence of WDFY2...
  51. Metabolic Signals Regulating GLUT4 Expression in vivo
    Ann Louise Olson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): GLUT4 is the isoform principally responsible for insulin-mediated glucose uptake in mammalian tissues. Glucose homeostasis is sensitive to changes in GLUT4 levels...
  52. JONATHAN BOGAN; Fiscal Year: 2016
    ..provided by applicant): Abstract Insulin stimulates glucose uptake in muscle by mobilizing intracellular GLUT4 storage vesicles (GSVs), which fuse at the cell surface and insert GLUT4 glucose transporters into the sarcolemma...
  53. Regulation of insulin sensitivity by TUG acetylation
    JONATHAN BOGAN; Fiscal Year: 2013
    ..test the overall hypothesis that acetylation regulates insulin sensitivity directly, by controlling the number of GLUT4 glucose transporters that reside in an intracellular, insulin-responsive pool...
  54. Genome-wide Association Study: Fetal Hemoglobin Phenotypes in Sickle Cell Disease
    Betty Pace; Fiscal Year: 2009
    ..aerobic metabolism, [unreadable]-oxidation, and mitochondrial biogenesis inclusive of PGC-1[unreadable], GLUT4, hexokinase, citrate synthase, beta-hydroxyacyl CoA dehydrogenase, and the serine phosphorylation of insulin ..
  55. Role of SH2-B in glucose metabolism
    Liangyou Rui; Fiscal Year: 2012
    ..Determine whether SH2B1 mediates insulin- stimulated glucose uptake in adipocytes by promoting GLUT4 trafficking. Aim 2...
  56. Insulin Sensitivity using Aerobic Interval Conditioning (ISAIC)
    Conrad P Earnest; Fiscal Year: 2010
    ..aerobic metabolism, [unreadable]-oxidation, and mitochondrial biogenesis inclusive of PGC-1[unreadable], GLUT4, hexokinase, citrate synthase, beta-hydroxyacyl CoA dehydrogenase, and the serine phosphorylation of insulin ..
  57. Alan R Saltiel; Fiscal Year: 2016
    ..Insulin stimulates glucose uptake in these cells by increasing the concentration of the facilitativ transporter Glut4 at the cell surface, and stimulates storage by increasing the conversion of glucose into lipid...
  58. Potassium channels in myotonic dystrophy type 1
    Christine Beeton; Fiscal Year: 2012
    ..3 and Kv1.5 channels. We have found that a blocker of both Kv1.3 and Kv1.5 channels improved GLUT4 translocation to the plasma membrane of human DM1 myoblasts in response to insulin. Blocking Kv1...
  59. Alvin C Powers; Fiscal Year: 2016
    ..studied after transplantation into immunodeficient mice that have genetic or dietary forms of insulin resistance (GLUT4 knockout, ob/ob mice, and high fat feeding)...
  60. Keyong Du; Fiscal Year: 2014
    ..Akt signaling;and c) ClipR-59 is complexed with AS160, a Rab-GAP protein that connects insulin signaling and Glut4 vesicles...
  61. Alan R Saltiel; Fiscal Year: 2016
    ..The exocyst complex was found in our laboratory to play a role in the regulation of glucose transport, targeting Glut4 vesicles to sites of docking and fusion in fat cells...
  62. Muhammad Abdul-Ghani; Fiscal Year: 2016
    ..glucose uptake (IMGU) with the stepped euglycemic insulin clamp, (ii) the protein content of insulin dependent (GLUT4/GLUT12) and glucose-mediated glucose transporters (GLUT1/GLUT3/GLUT11) and gene expression in skeletal muscle in ..
  63. ADIPOCYTE PROTEIN SECRETION AND INSULIN ACTION
    Harvey Lodish; Fiscal Year: 2007
    ..compartments in 3T3-L1 adipocytes including vesicles which transport membrane associated proteins like GLUT4, secreted proteins where secretion is stimulated by insulin such as ARCP30 (adipocyte complement related protein ..
  64. Role of Rab GAPs AS160 and Tbc1d1 in GLUT4 translocation and glucose homeostasis
    SUSANNA KELLER; Fiscal Year: 2009
    ..under fasting and fed conditions is the proper control of the subcellular distribution of the glucose transporter GLUT4 in muscle and fat cells...
  65. Insulin Signaling to GLUT4 and the Actin Cytoskeleton
    Michael Czech; Fiscal Year: 2007
    ..b>GLUT4 glucose transporters mediate the important physiological effect of insulin to stimulate glucose transport into fat ..
  66. Glucose Meatbolism in the Human Ovary
    MAUREEN CHARRON; Fiscal Year: 2004
    b>GLUT4 is the main insulin responsive glucose transporter in adipose and muscle which plays an important role in regulating glucose metabolism...
  67. GLUCOSE TRANSPORTER EXPRESSION IN TRANSGENIC MICE
    ANN OLSON; Fiscal Year: 1999
    The overall goal of this research is to examine the tissue-specific and hormonal/metabolic regulation of the GLUT4 facilitative glucose transporter gene in adipose tissue, heart and skeletal muscle...
  68. Mechanism of GLUT4 Inhibition by HIV Protease Inhibitor
    Paul Hruz; Fiscal Year: 2005
    ..The recent discovery that HIV protease inhibitors are capable of selectively inhibiting GLUT4, the major insulin responsive glucose transporter, is the first report that it is possible to selectively and ..