F2

Summary

Gene Symbol: F2
Description: coagulation factor II, thrombin
Alias: RPRGL2, THPH1, prothrombin, prepro-coagulation factor II, prothrombin B-chain, serine protease
Species: human

Top Publications

  1. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
  2. ncbi Role of thrombin and its major cellular receptor, protease-activated receptor-1, in pulmonary fibrosis
    D C Howell
    Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, University College London, Rayne Institute, 5 University Street, London WC1E 6JJ, U K
    Biochem Soc Trans 30:211-6. 2002
  3. doi D-dimer and prothrombin fragment 1 + 2 predict venous thromboembolism in patients with cancer: results from the Vienna Cancer and Thrombosis Study
    Cihan Ay
    Clinical Division of Haematology and Haemostaseology, Department of Medicine I, Medical University of Vienna, Waehringer GuerA 1090 Vienna, Austria
    J Clin Oncol 27:4124-9. 2009
  4. pmc PAR-1 and thrombin: the ties that bind the microenvironment to melanoma metastasis
    Maya Zigler
    Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
    Cancer Res 71:6561-6. 2011
  5. ncbi Influence of coagulation factor, vitamin K epoxide reductase complex subunit 1, and cytochrome P450 2C9 gene polymorphisms on warfarin dose requirements
    Christina L Aquilante
    Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL 32610, USA
    Clin Pharmacol Ther 79:291-302. 2006
  6. ncbi Platelet glycoprotein Ib alpha binds to thrombin anion-binding exosite II inducing allosteric changes in the activity of thrombin
    C Q Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:6161-8. 2001
  7. doi p38 MAPK controls prothrombin expression by regulated RNA 3' end processing
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology, and Immunology, University of Heidelberg, Heidelberg, Germany
    Mol Cell 41:298-310. 2011
  8. ncbi Protease-activated receptor-1 signaling by activated protein C in cytokine-perturbed endothelial cells is distinct from thrombin signaling
    Matthias Riewald
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:19808-14. 2005
  9. pmc N-linked glycosylation of protease-activated receptor-1 second extracellular loop: a critical determinant for ligand-induced receptor activation and internalization
    Antonio G Soto
    Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, California 92093 0636, USA
    J Biol Chem 285:18781-93. 2010
  10. ncbi Plasminogen activator inhibitor-1 and vitronectin promote the cellular clearance of thrombin by low density lipoprotein receptor-related proteins 1 and 2
    S Stefansson
    Biochemistry Department, J H Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    J Biol Chem 271:8215-20. 1996

Research Grants

  1. COMMON VARIANTS IN CANDIDATE GENES AND PREMATURE MI RISK
    Stephen Schwartz; Fiscal Year: 2001
  2. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    ALEXANDRA ROSE LUCAS; Fiscal Year: 2010
  3. Yves A DeClerck; Fiscal Year: 2016
  4. Mechanisms linking the hemostatic protease thrombin to arthritic disease
    Matthew J Flick; Fiscal Year: 2013
  5. PETER ROLF PANIZZI; Fiscal Year: 2016
  6. Membrane Receptors Regulating Megakaryocyte Endocytosis of Factor V from Plasma
    BETH BOUCHARD; Fiscal Year: 2012
  7. Alireza R Rezaie; Fiscal Year: 2015
  8. Engineering a chimeric serpin as a preventive therapeutic for ischemic stroke
    JILL C RAU; Fiscal Year: 2010
  9. William P Fay; Fiscal Year: 2016
  10. Albert Mulenga; Fiscal Year: 2014

Detail Information

Publications290 found, 100 shown here

  1. ncbi A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
    S R Poort
    Hemostasis and Thrombosis Research Center, Leiden University Hospital, The Netherlands
    Blood 88:3698-703. 1996
    We have examined the prothrombin gene as a candidate gene for venous thrombosis in selected patients with a documented familial history of venous thrombophilia...
  2. ncbi Role of thrombin and its major cellular receptor, protease-activated receptor-1, in pulmonary fibrosis
    D C Howell
    Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, University College London, Rayne Institute, 5 University Street, London WC1E 6JJ, U K
    Biochem Soc Trans 30:211-6. 2002
    ..Targeting the profibrotic effects of coagulation proteases warrants further evaluation as a potential therapeutic strategy for fibrotic lung disease...
  3. doi D-dimer and prothrombin fragment 1 + 2 predict venous thromboembolism in patients with cancer: results from the Vienna Cancer and Thrombosis Study
    Cihan Ay
    Clinical Division of Haematology and Haemostaseology, Department of Medicine I, Medical University of Vienna, Waehringer GuerA 1090 Vienna, Austria
    J Clin Oncol 27:4124-9. 2009
    ..The aim of this study was to investigate D-dimer and prothrombin fragment 1 + 2 (F 1 + 2), which reflect activation of blood coagulation and fibrinolysis, for prediction of ..
  4. pmc PAR-1 and thrombin: the ties that bind the microenvironment to melanoma metastasis
    Maya Zigler
    Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
    Cancer Res 71:6561-6. 2011
    ..Inhibiting such interactions by targeting PAR-1 could potentially be a useful therapeutic modality for melanoma patients...
  5. ncbi Influence of coagulation factor, vitamin K epoxide reductase complex subunit 1, and cytochrome P450 2C9 gene polymorphisms on warfarin dose requirements
    Christina L Aquilante
    Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL 32610, USA
    Clin Pharmacol Ther 79:291-302. 2006
    ....
  6. ncbi Platelet glycoprotein Ib alpha binds to thrombin anion-binding exosite II inducing allosteric changes in the activity of thrombin
    C Q Li
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:6161-8. 2001
    ....
  7. doi p38 MAPK controls prothrombin expression by regulated RNA 3' end processing
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology, and Immunology, University of Heidelberg, Heidelberg, Germany
    Mol Cell 41:298-310. 2011
    ..Although induced in many (patho-)physiological conditions, the underlying mechanisms controlling prothrombin expression remained enigmatic...
  8. ncbi Protease-activated receptor-1 signaling by activated protein C in cytokine-perturbed endothelial cells is distinct from thrombin signaling
    Matthias Riewald
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:19808-14. 2005
    ..These data may explain how APC can be therapeutically protective through the EPCR-PAR1 signaling despite ongoing thrombin generation due to disseminated intravascular coagulopathy...
  9. pmc N-linked glycosylation of protease-activated receptor-1 second extracellular loop: a critical determinant for ligand-induced receptor activation and internalization
    Antonio G Soto
    Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, California 92093 0636, USA
    J Biol Chem 285:18781-93. 2010
    ....
  10. ncbi Plasminogen activator inhibitor-1 and vitronectin promote the cellular clearance of thrombin by low density lipoprotein receptor-related proteins 1 and 2
    S Stefansson
    Biochemistry Department, J H Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    J Biol Chem 271:8215-20. 1996
    ..This pathway is potentially important for the regulation of the potent biological activities of thrombin, particularly at sites of vascular injury...
  11. pmc Caveolin-1 scaffold domain interacts with TRPC1 and IP3R3 to regulate Ca2+ store release-induced Ca2+ entry in endothelial cells
    Premanand C Sundivakkam
    Dept of Pharmacology M C 868 College of Medicine, Univ of Illinois 835 South Wolcott Ave, Chicago, IL 60612, USA
    Am J Physiol Cell Physiol 296:C403-13. 2009
    ..These findings suggest that CSD interacts with TRPC1 and IP(3)R3 and thereby regulates Ca(2+) store release-induced Ca(2+) entry in endothelial cells...
  12. ncbi Relations of Budd-Chiari syndrome to prothrombin gene mutation
    Guo Ling Lin
    Department of General Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
    Hepatobiliary Pancreat Dis Int 3:214-8. 2004
    ..This study was to explore whether prothrombin gene mutation (FII G20210A) takes part in the pathogenesis of BCS and to investigate their correlativity.
  13. ncbi The V3 loops of the HIV-1 and HIV-2 surface glycoproteins contain proteolytic cleavage sites: a possible function in viral fusion?
    G J Clements
    Celltech Ltd, Slough, Berkshire, England
    AIDS Res Hum Retroviruses 7:3-16. 1991
    ..We suggest that cleavage of the V3 loop by a cell surface or endosomal proteinase occurs during the HIV-cell fusion reaction, and that neutralizing antibodies directed against the V3 loop might act by inhibition of this reaction...
  14. pmc Essential role of cofilin-1 in regulating thrombin-induced RelA/p65 nuclear translocation and intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells
    Fabeha Fazal
    Department of Pediatrics Neonatology, Lung Biology and Disease Program, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 284:21047-56. 2009
    ..Together, these data show that cofilin-1 occupies a central position in RhoA-actin pathway mediating nuclear translocation of RelA/p65 and expression of ICAM-1 in endothelial cells...
  15. doi Thrombin enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts; possible involvement in the pathogenesis of preeclampsia
    Yin Zhao
    Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan
    Fertil Steril 98:917-21. 2012
    ..To investigate the possible impact of thrombin on soluble Fms-like tyrosine kinase 1 (sFlt-1) expression in trophoblasts...
  16. pmc Anticoagulant activity of a unique sulfated pyranosic (1->3)-β-L-arabinan through direct interaction with thrombin
    Paula V Fernandez
    Catedra de Quimica de Biomoleculas, Departamento de Biologia Aplicada y Alimentos, Facultad de Agronomia, Universidad de Buenos Aires, Av San Martin 4453, 1417 Buenos Aires, Argentina
    J Biol Chem 288:223-33. 2013
    ..These results show that this novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity by a mechanism different from those found previously for other sulfated polysaccharides and glycosaminoglycans...
  17. ncbi The interaction of thrombin with fibrinogen. A structural basis for its specificity
    M T Stubbs
    Max Planck Institut fur Biochemie, Martinsried bei Munchen, Federal Republic of Germany
    Eur J Biochem 206:187-95. 1992
    ..The multiple interactions between thrombin and fibrinogen provide an explanation for the narrow specificity of thrombin. Structural grounds can be put forward for certain congenital clotting disorders...
  18. ncbi Relationships between fibrinogen, plasminogen activator inhibitor-1, and their gene polymorphisms in current smokers with essential hypertension
    Maria Jastrzebska
    Department of Clinical Biochemistry and Laboratory Diagnostics, Pomeranian Medical University, Al Powstancow Wlkp 72, 70 111 Szczecin, Poland
    Thromb Res 110:339-44. 2003
    ....
  19. pmc Protease activated receptor 1 (PAR-1) activation by thrombin is protective in human pulmonary artery endothelial cells if endothelial protein C receptor is occupied by its natural ligand
    Jong Sup Bae
    Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, 1100 S Grand Blvd, St Louis, MO 63104, USA
    Thromb Haemost 100:101-9. 2008
    ..These findings may have important ramifications for understanding the mechanism of the participation of the vascular PAR-1 in pathophysiology of the inflammatory disorders...
  20. ncbi Low-density lipoprotein receptor-related protein 1 polymorphism 663 C > T affects clotting factor VIII activity and increases the risk of venous thromboembolism
    R Vormittag
    Division of Haematology and Haemostaseology, Department of Internal Medicine I, Medical University of Vienna, Vienna, Austria
    J Thromb Haemost 5:497-502. 2007
    ..A multifunctional endocytic receptor, low-density lipoprotein receptor-related protein 1 (LRP1), mediates cellular uptake and subsequent degradation of FVIII and may contribute to variations in FVIII levels...
  21. ncbi The role of plasminogen activator inhibitor-1 polymorphism, factor-V-Leiden, and prothrombin-20210 mutations in pulmonary thromboembolism
    I Kivilcim Oguzulgen
    Department of Pulmonary Medicine, Gazi University School of Medicine, Ankara, Turkey
    Clin Appl Thromb Hemost 15:73-7. 2009
    ..association of plasminogen activator inhibitor-1 gene polymorphism and its coexistence with factor-V-Leiden and prothrombin-20210 mutations in pulmonary thromboembolism...
  22. ncbi Osteopontin, a novel substrate for matrix metalloproteinase-3 (stromelysin-1) and matrix metalloproteinase-7 (matrilysin)
    R Agnihotri
    Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074, USA
    J Biol Chem 276:28261-7. 2001
    ..We predict that interaction of MMPs with OPN at tumor and wound healing sites in vivo may be a mechanism of regulation of OPN bioactivity...
  23. ncbi High prevalence of the prothrombin gene mutation in women with intrauterine growth retardation, abruptio placentae and second trimester loss
    M J Kupferminc
    Department of Obstetrics and Gynecology, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Israel
    Acta Obstet Gynecol Scand 79:963-7. 2000
    ..Our objective was to investigate the association between pregnancy complications and the guanine 20210 adenine (G20210A) mutation in prothrombin gene.
  24. ncbi Factor V Leiden and prothrombin gene G20210A mutations in Italian patients with Behçet's disease and deep vein thrombosis
    Mauro Silingardi
    Ospedale di Reggio Emilia, Regio Emilia, Italy
    Arthritis Rheum 51:177-83. 2004
    To evaluate the frequency and type of vascular lesions and to study the association of factor V gene G1691A (Leiden) and prothrombin gene G20210A polymorphisms with venous thrombosis in Italian patients with Behçet's disease (BD).
  25. pmc Thrombin induces tumor invasion through the induction and association of matrix metalloproteinase-9 and beta1-integrin on the cell surface
    A Reza Radjabi
    Department of Obstetrics and Gynecology Section of Gynecologic Oncology, Center for Integrative Science, University of Chicago, Chicago, IL 60637, USA
    J Biol Chem 283:2822-34. 2008
    The procoagulatory serine protease, thrombin, is known to induce invasion and metastasis in various cancers, but the mechanisms by which it promotes tumorigenesis are poorly understood...
  26. doi Inherited thrombophilic abnormalities and risk of portal vein thrombosis. a meta-analysis
    Francesco Dentali
    Department of Clinical Medicine, University of Insubria, Viale Borri 57, Varese, 21100, Italy
    Thromb Haemost 99:675-82. 2008
    ..It was the purpose of this study to assess the risk of PVT associated with factor V Leiden (FVL) and G20210A prothrombin mutation (PTM)...
  27. ncbi Thrombin inhibits tumor cell growth in association with up-regulation of p21(waf/cip1) and caspases via a p53-independent, STAT-1-dependent pathway
    Y Q Huang
    New York University Medical School and Kaplan Cancer Center, New York, New York 10016, USA
    J Biol Chem 275:6462-8. 2000
    ..These properties of thrombin rely on its action as a serine protease, which cleaves the N-terminal region of a 7-transmembrane G protein receptor (protease-activated receptor, ..
  28. ncbi Protease nexin-1 interacts with thrombomodulin and modulates its anticoagulant effect
    Marie Christine Bouton
    Unité INSERM U698, CHU Xavier Bichat, Paris, France
    Circ Res 100:1174-81. 2007
    ..Furthermore, PN-1 decreased the cofactor activity of TM on thrombin activable fibrinolysis inhibitor activation by thrombin. These data show for the first time that PN-1 forms complexes with TM and modulates its anticoagulant activity...
  29. ncbi Multimerin 1 binds factor V and activated factor V with high affinity and inhibits thrombin generation
    Samira B Jeimy
    Department of Pathology and Molecular Medicine, Health Sciences Center, McMaster University, 1200 Main St West, Hamilton, Ontario, Canada, L8N 3Z5
    Thromb Haemost 100:1058-67. 2008
    ..The high affinity binding of FV to MMRN1 may facilitate the costorage of the two proteins in platelet alpha-granules. As a consequence, MMRN1 release during platelet activation may limit platelet dependent thrombin generation in vivo...
  30. doi Role of Rac 1 and cAMP in endothelial barrier stabilization and thrombin-induced barrier breakdown
    Y Baumer
    Institute of Anatomy and Cell Biology, University of Wurzburg, Würzburg 97070, Germany
    J Cell Physiol 220:716-26. 2009
    ..Taken together, these data indicate that decrease of cAMP and Rac 1 activity may be an important step in inflammatory barrier disruption...
  31. pmc Long range communication between exosites 1 and 2 modulates thrombin function
    Nicolas S Petrera
    Department of Medicine, McMaster University, and Henderson Research Center, Hamilton, Ontario L8V 1C3, Canada
    J Biol Chem 284:25620-9. 2009
    ..When gamma(A)/gamma(A)-fibrinogen was clotted with thrombin in the presence of HD22, gamma'-peptide, or prothrombin fragment 2 there was a dose-dependent and saturable decrease in thrombin binding to the resultant fibrin clots...
  32. ncbi Reciprocal modulation of astrocyte stellation by thrombin and protease nexin-1
    K P Cavanaugh
    Department of Biochemistry, College of Medicine, University of California, Irvine 92717
    J Neurochem 54:1735-43. 1990
    ..Moreover, several other mitogens for astrocytes did not reverse dibutyryl cyclic AMP-induced stellation. Thus, the stellation reversal effect of thrombin is distinct from the mitogenic response...
  33. ncbi Interaction of coagulation defects and cardiovascular risk factors: increased risk of myocardial infarction associated with factor V Leiden or prothrombin 20210A
    C J Doggen
    Department of Clinical Epidemiology, Leiden University Hospital, Netherlands
    Circulation 97:1037-41. 1998
    A genetic variation located in the 3'-untranslated region of the prothrombin gene (prothrombin 20210 G-->A) was recently described as a risk factor for venous thrombosis...
  34. ncbi Proexosite-1 on prothrombin is a factor Va-dependent recognition site for the prothrombinase complex
    Lin Chen
    Edward A Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:27564-9. 2003
    ..exosite-1 to the catalytic function of thrombin has been studied extensively, their role in the specificity of prothrombin recognition by factor Xa in the prothrombinase complex (factor Xa, factor Va, phosphatidylcholine/..
  35. ncbi Intercellular adhesion molecule-1 (ICAM-1) expression is upregulated by thrombin in human monocytes and THP-1 cells in vitro and in pregnant subjects in vivo
    Peter Clark
    University Department of Obstetrics and Gynecology, Royal Infirmary, Glasgow, Scotland, UK
    Thromb Haemost 89:1043-51. 2003
    ....
  36. ncbi Role of prothrombin fragment 1 in the pathway of regulatory exosite I formation during conversion of human prothrombin to thrombin
    Patricia J Anderson
    Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 278:44489-95. 2003
    b>Prothrombin (Pro) activation by factor Xa generates the thrombin catalytic site and exosites I and II...
  37. ncbi Thrombin activates envelope glycoproteins of HIV type 1 and enhances fusion
    Hong Ling
    Division of Allergy and Infectious Diseases, Department of Internal Medicine, Graduate School of Medicine, Tohoku University, 1 1Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    Microbes Infect 6:414-20. 2004
    ..001) was observed. In conclusion, thrombin, one of the major inflammatory molecules in blood, facilitates HIV-induced cell fusion, probably by activating gp120...
  38. ncbi Thrombin modulates the expression of a set of genes including thrombospondin-1 in human microvascular endothelial cells
    Joseph N McLaughlin
    Department of Pharmacology, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 444 Robinson Research Building, 23rd Avenue South at Pierce, Nashville, TN 37232, USA
    J Biol Chem 280:22172-80. 2005
    ....
  39. ncbi The interaction of fragment 1 of prothrombin with the membrane surface is a prerequisite for optimum expression of factor Va cofactor activity within prothrombinase
    Michael A Bukys
    Cleveland State University, Department of Chemistry, OH 44115, USA
    Thromb Haemost 99:511-22. 2008
    Incorporation of factor (F) Va into prothrombinase directs prothrombin activation by FXa through the meizothrombin pathway, characterized by initial cleavage at Arg(320)...
  40. pmc Molecular basis of thrombin recognition by protein C inhibitor revealed by the 1.6-A structure of the heparin-bridged complex
    Wei Li
    Department of Haematology, Division of Structural Medicine, Thrombosis Research Unit, Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    Proc Natl Acad Sci U S A 105:4661-6. 2008
    ..These data significantly improve our understanding of the cofactor-dependent roles of PCI in hemostasis...
  41. ncbi Nucleotide sequence of the gene for human prothrombin
    S J Degen
    Children s Hospital Research Foundation, Cincinnati, Ohio
    Biochemistry 26:6165-77. 1987
    A human genomic DNA library was screened for the gene coding for human prothrombin with a cDNA coding for the human protein. Eighty-one positive lambda phage were identified, and three were chosen for further characterization...
  42. ncbi Polymorphisms in the prothrombin gene and their association with plasma prothrombin levels
    H Ceelie
    Haemostasis and Thrombosis Research Centre, Dept of Haematology, Leiden University Medical Centre, The Netherlands
    Thromb Haemost 85:1066-70. 2001
    To find genetic causes of high plasma prothrombin levels, an established prothrombotic risk factor. we searched for sequence variations in the prothrombin gene...
  43. ncbi Thrombin functions during tissue factor-induced blood coagulation
    Kathleen E Brummel
    Department of Biochemistry, University of Vermont, College of Medicine, Burlington, VT 05405, USA
    Blood 100:148-52. 2002
    ..time with inhibitors, the soluble phases were analyzed for thrombin-antithrombin III (TAT) complex formation, prothrombin fragments, platelet activation (osteonectin release), factor Va generation, fibrinopeptide (FP) A and FPB ..
  44. ncbi The molecular basis of thrombin allostery revealed by a 1.8 A structure of the "slow" form
    James A Huntington
    Department of Haematology, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Hills Road, CB2 2XY, Cambridge, United Kingdom
    Structure 11:469-79. 2003
    ..These residues constitute an allosteric switch, which is flipped directly through sodium binding, resulting in the fast form with an open active site...
  45. ncbi Role of factor V Leiden and prothrombin 20210A in patients with retinal artery occlusion
    M Weger
    Department of Ophthalmology Karl Franzens University Graz, Austria
    Eye (Lond) 17:731-4. 2003
    ..Numerous studies have shown that two genetic variants, factor V Leiden and prothrombin 20210A, cause a procoagulant state...
  46. ncbi The intronic prothrombin 19911A>G polymorphism influences splicing efficiency and modulates effects of the 20210G>A polymorphism on mRNA amount and expression in a stable reporter gene assay system
    Nicolas von Ahsen
    Georg August University, Department of Clinical Chemistry, Robert Koch Str 40, 37075 Gottingen, Germany
    Blood 103:586-93. 2004
    The common prothrombin gene cleavage site mutation 20210G>A is associated with elevated prothrombin levels and thrombosis...
  47. ncbi G20210A is a functional mutation in the prothrombin gene; effect on protein levels and 3'-end formation
    H Ceelie
    Hemostasis and Thrombosis Research Center, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands
    J Thromb Haemost 2:119-27. 2004
    The prothrombin G20210A mutation is associated with increased plasma prothrombin levels and risk of thrombosis...
  48. ncbi Genetic thrombophilias and preeclampsia: a meta-analysis
    Julie Lin
    Renal Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Obstet Gynecol 105:182-92. 2005
    ..single nucleotide polymorphism (SNP), the methylene tetrahydrofolate reductase (MTHFR) 677 C-T SNP, and the prothrombin 20210 G-A SNP and the risk of preeclampsia, by conducting a meta-analysis of all case-control studies with data ..
  49. ncbi [Factor V Leiden (G1691A) and prothrombin-G20210A alleles among patients with deep venous thrombosis and in the general population from Spain]
    Francesc Frances
    Unidad de Epidemiología Genética y Molecular, Departament de Medicina Preventiva, Universitat de Valencia, Espana
    Rev Med Chil 134:13-20. 2006
    Factor V leiden and the -G20210A variant of prothrombin gene are associated to a higher risk of deep venous thrombosis.
  50. ncbi Screening for thrombophilia in high-risk situations: systematic review and cost-effectiveness analysis. The Thrombosis: Risk and Economic Assessment of Thrombophilia Screening (TREATS) study
    O Wu
    Division of Developmental Medicine, University of Glasgow, UK
    Health Technol Assess 10:1-110. 2006
    ..To evaluate the relative cost-effectiveness of universal and selective VTE history-based screening for thrombophilia compared with no screening in the three high-risk patient groups...
  51. ncbi Sialylation of urinary prothrombin fragment 1 is implicated as a contributory factor in the risk of calcium oxalate kidney stone formation
    Dawn Webber
    Department of Chemistry, University of Cape Town, South Africa
    FEBS J 273:3024-37. 2006
    ..A previous study involving urinary prothrombin fragment 1 from both populations demonstrated superior inhibitory activity associated with the protein from the ..
  52. ncbi Prothrombin gene 20209 C >T along with the first description of a homozygous polymorphism at the 3' downstream region +4 C >T in the Turkish population
    Hilal Ozdag
    Department of Ankara University, Pediatric Molecular Genetics, Cayyolu 06810, Ankara, Turkey
    Lab Hematol 12:131-3. 2006
    Mutation screening studies related with thrombosis revealed that prothrombin 20210A allele of the prothrombin gene leads to an increase in plasma prothrombin levels...
  53. pmc Splicing factors stimulate polyadenylation via USEs at non-canonical 3' end formation signals
    Sven Danckwardt
    Department of Pediatric Oncology, Hematology and Immunology, University of Heidelberg, Germany
    EMBO J 26:2658-69. 2007
    The prothrombin (F2) 3' end formation signal is highly susceptible to thrombophilia-associated gain-of-function mutations...
  54. ncbi Inheritance and perinatal consequences of inherited thrombophilia in Greece
    Marina Karakantza
    Department of Internal Medicine, Division of Hematology, University of Patras Medical School, Patras, Greece
    Int J Gynaecol Obstet 100:124-9. 2008
    ..To investigate the impact of inherited thrombophilic factors on the gestational outcome of unselected pregnant women...
  55. ncbi Glycosylation of prothrombin fragment 1 governs calcium oxalate crystal nucleation and aggregation, but not crystal growth
    Dawn Webber
    Department of Chemistry, University of Cape Town, Private Bag, Rondebosch, Cape Town, 7701, South Africa
    Urol Res 35:277-85. 2007
    ..In several cases, this has been attributed to glycosylation of the proteins as evidenced by urinary prothrombin fragment 1 where there is a correlation between sialylation and calcium oxalate kidney stone disease...
  56. doi Factor V Leiden is associated with pre-eclampsia but not with fetal growth restriction: a genetic association study and meta-analysis
    T Dudding
    Hunter Genetics, Hunter New England Health Service, NSW, Australia
    J Thromb Haemost 6:1869-75. 2008
    ..factor V gene, the 1691G to A change (rs6025) located on chromosome 1q23 (factor V Leiden, FVL), and the other in the prothrombin gene, 20210G to A change (rs1799963) on chromosome 11p11-q12 (PT). These results, however, are conflicting.
  57. doi The effects of exosite occupancy on the substrate specificity of thrombin
    Natasha May Yoke Ng
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
    Arch Biochem Biophys 489:48-54. 2009
    ..Thus, exosite I ligands do affect the activity and specificity of thrombin, but not greatly enough to explain the altered substrate profile of the enzyme when complexed with thrombomodulin...
  58. doi Apolipoprotein C-III predicts cardiovascular mortality in severe coronary artery disease and is associated with an enhanced plasma thrombin generation
    O Olivieri
    Department of Clinical and Experimental Medicine, Unit of Internal Medicine, University of Verona, Verona, Italy
    J Thromb Haemost 8:463-71. 2010
    ..Apolipopoprotein C-III (apo C-III) plays a pivotal role in controlling plasma triglyceride (TG) and contributes to the atherogenic properties of TG-rich lipoproteins...
  59. pmc Brain endothelial cells synthesize neurotoxic thrombin in Alzheimer's disease
    Xiangling Yin
    Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Am J Pathol 176:1600-6. 2010
    ....
  60. doi Combined measurement of preoperative α-fetoprotein and des-γ-carboxy prothrombin predicts recurrence after curative resection in patients with hepatitis-B-related hepatocellular carcinoma
    Young Eun Chon
    Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
    Int J Cancer 131:2332-41. 2012
    Alpha-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are widely used complementary tumor markers for hepatocellular carcinoma (HCC)...
  61. ncbi Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation
    T K Vu
    Department of Medicine, University of California, San Francisco 94143 0524
    Cell 64:1057-68. 1991
    ..These data reveal a novel signaling mechanism in which thrombin cleaves its receptor's amino-terminal extension to create a new receptor amino terminus that functions as a tethered ligand and activates the receptor...
  62. ncbi The structure of a complex of recombinant hirudin and human alpha-thrombin
    T J Rydel
    Department of Chemistry, Michigan State University, East Lansing 48824
    Science 249:277-80. 1990
    ..In all, 27 of the 65 residues of hirudin have contacts less than 4.0 A with thrombin (10 ion pairs and 23 hydrogen bonds). Such abundant interactions may account for the high affinity and specificity of hirudin...
  63. ncbi The roles of protein C and thrombomodulin in the regulation of blood coagulation
    C T Esmon
    Howard Hughes Medical Institute, Oklahoma Medical Research Foundation, Oklahoma City 73104
    J Biol Chem 264:4743-6. 1989
  64. pmc The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment
    W Bode
    Max Planck Institut fur Biochemie, Martinsried, FRG
    EMBO J 8:3467-75. 1989
    ..The narrow active site cleft, with a more polar base and hydrophobic rims, extends towards the arginine-rich surface of loop Lys70-Glu80 that probably represents part of the anionic binding region for hirudin and fibrinogen...
  65. ncbi Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively
    N J Royle
    Somat Cell Mol Genet 13:285-92. 1987
    The gene for human prothrombin, or factor II (F2) has been assigned to 11p11-q12 by the combined use of a panel of somatic cell hybrid DNAs and in situ hybridization, using both cDNA and genomic probes...
  66. ncbi A common prothrombin variant (20210 G to A) increases the risk of myocardial infarction in young women
    F R Rosendaal
    Department of Clinical Epidemiology, University Hospital Leiden, The Netherlands
    Blood 90:1747-50. 1997
    ..These results, in which the effect of major coronary risk factors is enhanced fourfold to sixfold by the prothrombin variant, are similar to those previously reported for another genetic clotting abnormality, factor V Leiden...
  67. ncbi The factor XIII V34L polymorphism accelerates thrombin activation of factor XIII and affects cross-linked fibrin structure
    R A Ariëns
    Unit of Molecular Vascular Medicine, University of Leeds School of Medicine, UK
    Blood 96:988-95. 2000
    ..The structural differences were confirmed by electron microscopy. These results demonstrate that Val34Leu accelerates activation of FXIII by thrombin and consequently affects the structure of the cross-linked fibrin clot...
  68. ncbi Abdominal venous thrombosis in neonates and infants: role of prothrombotic risk factors - a multicentre case-control study. For the Childhood Thrombophilia Study Group
    C Heller
    Department of Paediatrics, University of Frankfurt, Germany
    Br J Haematol 111:534-9. 2000
    The factor V (FV) G1691A mutation, the prothrombin (PT) G20210A variant, the methylenetetrahydrofolate reductase (MTHFR) T677T genotype, together with fasting homocysteine (HCY) concentration, lipoprotein (Lp)(a), anti-thrombin (AT), ..
  69. ncbi G20210A prothrombin gene polymorphism and prothrombin activity in subjects with or without angiographically documented coronary artery disease
    C Russo
    Department of Clinical and Experimental Medicine, University of Verona, Verona, Italy
    Circulation 103:2436-40. 2001
    G20210A prothrombin mutation has been associated with high prothrombin levels and an increased risk of venous thrombosis...
  70. ncbi Thrombin induces increased expression and secretion of angiopoietin-2 from human umbilical vein endothelial cells
    Yao Qi Huang
    Department of Medicine and Kaplan Cancer Center, New York University Medical School, New York, NY 10016, USA
    Blood 99:1646-50. 2002
    ..Thus, thrombin-induced tumorigenesis and metastasis is associated with enhanced Ang-2 protein synthesis and secretion via enhanced transcription of Ang-2. This could help explain how thrombin promotes angiogenesis...
  71. ncbi Frequency of factor V, prothrombin and methylenetetrahydrofolate reductase gene variants in preeclampsia
    Angela V D'Elia
    Department of Biomedical Science and Technology, University of Udine, Udine, Italy
    Gynecol Obstet Invest 53:84-7. 2002
    The association between thrombophilic variants (Leiden mutation of the factor V gene, G20210A mutation of the prothrombin gene and C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene) with preeclampsia was ..
  72. ncbi Prothrombotic genotypes are not associated with pre-eclampsia and gestational hypertension: results from a large population-based study and systematic review
    E R Morrison
    Department of Medicine and Therapeutics, University of Aberdeen, Scotland
    Thromb Haemost 87:779-85. 2002
    ..The polymorphisms studied were: G1691A in Factor V (Factor V Leiden; FVL), prothrombin G20210A, methylenetetrahydrofolate reductase (MTHFR) C677T, plasminogen activator inhibitor-1 4G/5G and the ..
  73. ncbi Prothrombin G20210A is a bifunctional gene polymorphism
    Angela M Carter
    Academic Unit of Molecular Vascular Medicine, University of Leeds, United Kingdom
    Thromb Haemost 87:846-53. 2002
    The G20210A polymorphism has been shown to alter the efficiency of prothrombin mRNA processing. Here we show that the G20210A mutation also alters prothrombin mRNA stability...
  74. ncbi Factor V gene G1691A mutation, prothrombin gene G20210A mutation, and MTHFR gene C677T mutation are not risk factors for pulmonary thromboembolism in Chinese population
    Yanhui Lu
    Division of Cardio Pulmonary Circulation, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 100037, Beijing, People s Republic of China
    Thromb Res 106:7-12. 2002
    A mutation in coagulant factor V gene, a substitution in the 3' untranslated region of prothrombin gene, and a variant in 5,10-methylenetetrahydrofolate reductase (MTHFR) gene have been reported to be related to venous thromboembolism in ..
  75. ncbi Des-gamma-carboxy prothrombin in cancer and non-cancer liver tissue of patients with hepatocellular carcinoma
    Wei Tang
    Hepato Biliary Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Int J Oncol 22:969-75. 2003
    Des-gamma-carboxy prothrombin (DCP), also known as protein induced by vitamin K absence or antagonist II absence (PIVKA-II), has been considered as a useful serum tumor marker for hepatocellular carcinoma (HCC)...
  76. ncbi Modulation of alpha-thrombin function by distinct interactions with platelet glycoprotein Ibalpha
    Reha Celikel
    Roon Research Center for Arteriosclerosis and Thrombosis, Division of Experimental Thrombosis and Hemostasis, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 301:218-21. 2003
    ..These interactions may modulate alpha-thrombin function by mediating GpIbalpha clustering and cleavage of protease-activated receptors, which promote platelet activation, while limiting fibrinogen clotting through blockade of exosite I...
  77. ncbi Crystal structure of the GpIbalpha-thrombin complex essential for platelet aggregation
    John J Dumas
    Department of Chemical and Screening Sciences, Wyeth, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    Science 301:222-6. 2003
    ..The details of these interactions reconcile GpIbalpha-thrombin binding modes that are presently controversial,highlighting two distinct interfaces that are potential targets for development of novel antithrombotic drugs...
  78. ncbi [Occurrence of gene mutations in factor V Leiden, prothrombin and methylenetetrahydrofolate reductase in patients with pre-eclampsia]
    M Prochazka
    Klinika porodnictví a gynekologie LF UP v Olomouci
    Ceska Gynekol 68:162-6. 2003
    The aim of the study was to detect the incidence of thrombophilia FV Leiden, prothrombin G20210A, MTHFR C677T, resistance to activated protein C, and deficiency of protein C and S in pregnant or puerperal women with preeclampsia and ..
  79. pmc Prothrombin gene variants in non-Caucasians with fetal loss and intrauterine growth retardation
    Iris Schrijver
    Department of Pathology, Stanford University Medical Center, Stanford, California 94305, USA
    J Mol Diagn 5:250-3. 2003
    ..Two recently identified prothrombin gene mutations (20209C>T and 20221C>T) have been observed in non-Caucasian patients with thrombosis...
  80. ncbi Hyperprothrombinemia associated with prothrombin G20210A mutation inhibits plasma fibrinolysis through a TAFI-mediated mechanism
    Mario Colucci
    Department of Biomedical Sciences, Section of General Pathology, University of Bari, Italy
    Blood 103:2157-61. 2004
    The prothrombin gene mutation G20210A is a common risk factor for thrombosis and is associated with increased prothrombin levels. However, the mechanism whereby hyperprothrombinemia predisposes to thrombosis remains unclear...
  81. pmc G20210A prothrombin gene polymorphism and coronary ischaemic syndromes: a phenotype-specific meta-analysis of 12 034 subjects
    F Burzotta
    Institute of Cardiology, Catholic University, Rome, Italy
    Heart 90:82-6. 2004
    To investigate the possible link between the G20210A prothrombin gene variant and different forms of ischaemic heart disease.
  82. ncbi The significance of circulating factor IXa in blood
    Saulius Butenas
    Department of Biochemistry, University of Vermont, Burlington, Vermont 05405 0068, USA
    J Biol Chem 279:22875-82. 2004
    ....
  83. ncbi The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations
    Sven Danckwardt
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Blood 104:428-35. 2004
    The functional analysis of the common prothrombin 20210 G>A(F2 20210(*)A) mutation has recently revealed gain of function of 3'end processing as a novel genetic mechanism predisposing to human disease...
  84. ncbi Molecular dissection of Na+ binding to thrombin
    Agustin O Pineda
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 279:31842-53. 2004
    ..The role of the water network uncovered in this study establishes a new paradigm for the allosteric regulation of thrombin and other Na(+)-activated enzymes involved in blood coagulation and the immune response...
  85. ncbi Meta-analysis of genetic studies in ischemic stroke: thirty-two genes involving approximately 18,000 cases and 58,000 controls
    Juan P Casas
    Centre for Clinical Pharmacology, British Heart Foundation Laboratories at University College London, London, England
    Arch Neurol 61:1652-61. 2004
    ..33; 95% CI, 1.12-1.58), methylenetetrahydrofolate reductase C677T (OR, 1.24; 95% CI, 1.08-1.42), prothrombin G20210A (OR, 1.44; 95% CI, 1.11-1.86), and angiotensin-converting enzyme insertion/deletion (OR, 1...
  86. ncbi PAR1 is a matrix metalloprotease-1 receptor that promotes invasion and tumorigenesis of breast cancer cells
    Adrienne Boire
    Molecular Oncology Research Institute, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Cell 120:303-13. 2005
    ..These results demonstrate that MMP-1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion...
  87. ncbi The G20210A prothrombin-gene mutation and the plasminogen activator inhibitor (PAI-1) 5G/5G genotype are associated with early onset of severe preeclampsia
    A Gerhardt
    Department of Obstetrics and Gynecology, Heinrich Heine University Medical Center, Dusseldorf, Germany
    J Thromb Haemost 3:686-91. 2005
    ..9, 95% confidence interval (CI) 0.4, 2.2; prothrombin mutation, OR 1.9, 95% CI 0.5, 7.0; methylentetrahydrofolate reductase 677TT genotype, OR 0.8, 95% CI 0.4, 1...
  88. ncbi A case control study on the contribution of factor V-Leiden, prothrombin G20210A, and MTHFR C677T mutations to the genetic susceptibility of deep venous thrombosis
    Wassim Y Almawi
    Al Jawhara Center for Molecular Medicine, Genetics, and Inherited Diseases, Arabian Gulf University, Manama, Bahrain
    J Thromb Thrombolysis 19:189-96. 2005
    Insofar as the inherited prothrombotic single nucleotide polymorphisms (SNPs) factor V G1691A (FV-Leiden), prothrombin (PRT) G20210A, and methylenetetrahydrofolate reductase (MTHFR), C677T are inherited risk factors of venous ..
  89. ncbi The relationship of the factor V Leiden mutation and pregnancy outcomes for mother and fetus
    Donna Dizon-Townson
    National Institute of Child Health and Human Development Maternal Fetal Medicine Units Network, Bethesda, Maryland, USA
    Obstet Gynecol 106:517-24. 2005
    ....
  90. ncbi Low frequency of factor V Leiden and prothrombin G20210A mutations in patients with hepatic venous outflow tract obstruction in northern India: a case-control study
    Sirish I Kumar
    Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow
    Indian J Gastroenterol 24:211-5. 2005
    Factor V Leiden (FVL) and prothrombin gene (G20210A) mutations are known to be associated with venous thromboembolism. Several studies have shown an association of these mutations with hepatic venous outflow tract obstruction (HVOTO)...
  91. ncbi Prevalence of factor V Leiden and prothrombin G20210A mutations in Chinese patients with deep venous thrombosis and pulmonary embolism
    Z J Jun
    China Japan Union Hospital, Ji Lin University, Changchun, China
    Clin Lab Haematol 28:111-6. 2006
    ..Several genetic risk factors, especially factor V Leiden and prothrombin G20210A mutations have been reported to be related to VTE in Caucasians, but the relationship remains ..
  92. ncbi The relationship between thrombophilic mutations and preeclampsia: a prospective case-control study
    Ahmet Yalinkaya
    Dicle University School of Medicine, Department of Obstetrics and Gynecology, Diyarbakir, Turkey
    Ann Saudi Med 26:105-9. 2006
    ..The aim of this study was to determine the relationship between thrombophilia and preeclamptic patients in our region...
  93. ncbi Functional analysis of two prothrombin 3'-untranslated region variants: the C20209T variant, mainly found among African-Americans, and the C20209A variant
    H H A G M van der Putten
    J Thromb Haemost 4:2285-7; author reply 2288-9. 2006
  94. ncbi Hyperhomocysteinaemia and factor V Leiden mutation are associated with Budd-Chiari syndrome
    Yusuf Colak
    Department of Gastroenterology, Ege University Medical School, Izmir, Turkey
    Eur J Gastroenterol Hepatol 18:917-20. 2006
    ..We aimed to study the role of hyperhomocysteinaemia, factor V Leiden mutation and G20210A prothrombin gene mutation in the pathogenesis of the syndrome.
  95. ncbi Relationship between polymorphisms in thrombophilic genes and preeclampsia in a Brazilian population
    C A Dalmáz
    Departamento de Genetica, Instituto de Biociencias, Universidade Federal do Rio Grande do Sul, Caixa Postal 15053, 91 501 970 Porto Alegre, RS, Brazil
    Blood Cells Mol Dis 37:107-10. 2006
    ..07, 95% confidence interval (CI) 0.99-4.30; prothrombin mutation (F II) (GA or AA genotypes) OR 8.11, 95% CI 0.89-73.92; factor V Leiden (FV Leiden) OR 3.94, 95% CI 0...
  96. ncbi Inherited thrombophilias and pre-eclampsia in Brazilian women
    Luci Maria SantAna Dusse
    Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
    Eur J Obstet Gynecol Reprod Biol 134:20-3. 2007
    ..The aim of the present study was to compare the distribution of G1691A, G20210A and C677T mutations in pre-eclamptic Brazilian women and in matched control women with an uncomplicated normal pregnancy...
  97. ncbi Thrombin generation and fibrin clot structure
    Alisa S Wolberg
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, 816A Brinkhous Bullitt Building, CB 7525, Chapel Hill, NC 27599 7525, United States
    Blood Rev 21:131-42. 2007
    ..These findings suggest that studies explicitly evaluating fibrin formation during in situ thrombin generation are warranted to explain and fully appreciate mechanisms of normal and abnormal fibrin clot formation in vivo...
  98. ncbi Homozygosity for the C46T polymorphism of the F12 gene is a risk factor for venous thrombosis during the first pregnancy
    E Cochery-Nouvellon
    Haematology Laboratory, University Hospital, Nimes, France
    J Thromb Haemost 5:700-7. 2007
    A first thromboembolic event during pregnancy and puerperium is predisposed to by polymorphisms G1691A in the factor V gene (F5) (F5G1691A) and G20210A in the prothrombin gene (F2) (F2G20210A).
  99. ncbi Atypical mutations of the prothrombin gene at positions 20,209 and 20,218, and a novel mutation at position 20,219. Report on 10 patients
    C Flaujac
    J Thromb Haemost 5:1064-8. 2007
  100. ncbi CYP2C9 and VKORC1 genetic polymorphism analysis might be necessary in patients with Factor V Leiden and prothrombin gene G2021A mutation(s)
    Allen Leung
    Molecular Pathology Laboratory, Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467, USA
    Diagn Mol Pathol 16:184-6. 2007
    ..Factor V Leiden gene (most common) and the prothrombin G20210A gene mutation are inherited mild to moderate risk factors for hypercoagulability...

Research Grants67

  1. COMMON VARIANTS IN CANDIDATE GENES AND PREMATURE MI RISK
    Stephen Schwartz; Fiscal Year: 2001
    ....
  2. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    ALEXANDRA ROSE LUCAS; Fiscal Year: 2010
    ..to provide 'proof of principal'for therapeutic intervention with a new class of anti-inflammatory serine protease inhibitor (serpin) therapeutics...
  3. Yves A DeClerck; Fiscal Year: 2016
    ..provided by applicant): Plasminogen activator inhibitor-1 (PAI-1) is a member of the family of endogenous serine protease inhibitors (serpins) that controls the activation of plasminogen into plasmin by tissue and urokinase type ..
  4. Mechanisms linking the hemostatic protease thrombin to arthritic disease
    Matthew J Flick; Fiscal Year: 2013
    ..Activation of the hemostatic system and the subsequent conversion of prothrombin to the active serine protease thrombin is a prominent feature of RA...
  5. PETER ROLF PANIZZI; Fiscal Year: 2016
    ..Labeling ProT's serine protease active site with a fluorochrome allowed us to detect and monitor S...
  6. Membrane Receptors Regulating Megakaryocyte Endocytosis of Factor V from Plasma
    BETH BOUCHARD; Fiscal Year: 2012
    ..catalyzed by the prothrombinase complex, a 1:1, Ca2+-dependent complex of the cofactor factor Va and the serine protease factor Xa bound to activated platelets adhered to sites of vascular injury...
  7. Alireza R Rezaie; Fiscal Year: 2015
    DESCRIPTION (provided by applicant): The proteolytic activity of coagulation proteases is regulated by the serine protease inhibitors (serpins) in plasma...
  8. Engineering a chimeric serpin as a preventive therapeutic for ischemic stroke
    JILL C RAU; Fiscal Year: 2010
    ..Thrombin is a serine protease that is the ultimate enzyme in the coagulation cascade...
  9. William P Fay; Fiscal Year: 2016
    ..Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor and a major regulator of fibrinolysis and cell migration...
  10. Albert Mulenga; Fiscal Year: 2014
    ..are identification, characterization and validation of Amblyomma americanum and Ixodes scapularis tick saliva serine protease inhibitors (serpins) as vaccine target antigens for immunization of white tailed deer against tick ..
  11. Novel Protective Antiapoptotic Action of Alpha 1-Antitrypsin In Emphysema
    Rubin M Tuder; Fiscal Year: 2011
    ..in patients with alpha-1 antitrypsin (A1AT) deficiency emphasizes the role of A1AT as an elastase inhibitor, serine protease inhibitors (Serpins), including A1 AT, have broader biological effects beyond their classical action as ..
  12. Michael R Kanost; Fiscal Year: 2016
    ..sexta genome sequence and new developments in proteomics technology make possible the investigation of serine protease webs in M. sexta hemolymph, using biochemical systems approaches...
  13. Neuroprotection with serpins during cardiac surgery
    Richard A Jonas; Fiscal Year: 2010
    ..Aprotinin, a "SERPIN" (serine protease inhibitor), reduces bleeding after cardiopulmonary bypass (CPB)...
  14. Characterization of the An. gambiae LL3/SRPN6 pathway in response to Plasmodium i
    RYAN CHAD SMITH; Fiscal Year: 2012
    ..Recent studies have demonstrated that a mosquito serine protease inhibitor, serpin 6 (SRPN6), limits parasite success during the invasion of the midgut and salivary gland ..
  15. Alternaria proteases and protease defenses in asthma
    Michael O Daines; Fiscal Year: 2010
    ..There are endogenous inhibitors of proteases, including SERine Protease INhibitors (Serpins) and Stefin protease inhibitors, which are differentially upregulated with Alternaria ..
  16. Michael O Daines; Fiscal Year: 2015
    ..There are endogenous inhibitors of proteases, including SERine Protease INhibitors (Serpins) and Stefin protease inhibitors...
  17. RECOMBINANT SERPINS AND NEUTROPHILS IN ACUTE LUNG INJURY
    Michael Plotnick; Fiscal Year: 2000
    ..program which will contribute to our understanding of the biochemical properties and biological functions of serine protease inhibitor (serpins) as well as provide me with a foundation for a career as an independent investigator in ..
  18. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2004
    ..An imbalance between serine protease activity and inhibition generates biochemical cascades that often establish and propagate the ..
  19. Cross-talk Between Coagulation, Inflammation, and Immunity
    Lawrence L Leung; Fiscal Year: 2013
    ..that prochemerin, a novel chemoattractant whose activity is dependent on C-terminal proteolytic cleavage by serine protease(s), can be activated by sequential cleavages by plasmin and CPB, thus establishing a new substrate for CPB ..
  20. Fabeha Fazal; Fiscal Year: 2014
    ..applicant): The overall objective of the proposed studies is to address the mechanisms by which thrombin, a serine protease released during clotting initiated by sepsis or vascular injury, regulates the expression of adhesive protein ..
  21. Patrick D Lyden; Fiscal Year: 2016
    ..Thrombin is a serine protease that plays a critical role in the coagulation cascade...
  22. 2012 New Insights into Coagulation
    Eleanor Pollak; Fiscal Year: 2012
    ..Krishnaswamy, PhD and Rodney Camire, PhD will detail the workings of the scientific basis for the effective serine protease-based medications...
  23. Thomas H Smith; Fiscal Year: 2015
    ..Thrombin is a serine protease mediates hemostasis, thrombosis, and inflammatory responses to vascular injury and contributes to the ..
  24. TIMOTHY OLEARY; Fiscal Year: 2015
    ..The anticoagulant activity of heparin is largely derived from its action on the serine protease inhibitr antithrombin (AT)...
  25. G-protein-induced Signaling of PMN-mediated Lung Injury
    Arshad Rahman; Fiscal Year: 2010
    ..studies in this renewal application is to define the mechanisms by which the procoagulant thrombin, a serine protease released during clotting initiated by sepsis or vascular injury, regulates the expression of adhesion ..
  26. PROTEINASE SYSTEMS IN INSECT HEMOLYMPH
    Michael R Kanost; Fiscal Year: 2010
    Extracellular serine protease cascades modulate protective responses to limit bleeding and infection in vertebrate and invertebrate animals, but our understanding of these pathways in insects is rudimentary at best...
  27. EILEEN REMOLD-O'DONNELL; Fiscal Year: 2014
    ..We will determine whether the require NSP is located on the cell surface by the use of non-permeable serine protease inhibitors...
  28. NAPS2 Continuation Genome Wide Association Study of Pancreatitis
    DAVID CLEMENT WHITCOMB; Fiscal Year: 2011
    ..We demonstrated strong epistasis between CFTR and serine protease inhibitor Kazal type 1 (SPINK1) variants as a model of complex traits...
  29. Kristin Michel; Fiscal Year: 2014
    ..The long-term goal is to develop a malaria vector control methodology based on a new insecticide target, the serine protease inhibitor serpin (SRPN)2 from the African malaria mosquito, Anopheles gambiae s.s....
  30. Regulation of Pal-1 in Atherosclerosis and Thrombosis
    Yolanda M Fortenberry; Fiscal Year: 2013
    ..and hypertension. Elevated plasma levels of the serine protease inhibitor, plasminogen activatoMnhibitor-1, (PAI- 1) are correlated with these'risk factors and:several ..
  31. Indranil Biswas; Fiscal Year: 2015
    ..The Clp family is composed of a small cytoplasmic serine protease, called ClpP, and various ATPases. S...
  32. A novel diagnostic biomarker for hepatocellular carcinoma and cholangiocarcinoma
    Xuanyong Lu; Fiscal Year: 2012
    ..Background and Objective: Recent studies have suggested that levels of serine protease inhibitor Kazal (SPIK) is highly related to HCC/ICC progression and over- expression of SPIK is correlated to ..
  33. Anti-Inflammatory mechanisms of soybean-derived Bowman-Birk protease inhibitor
    Abdolmohamad Rostami; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Bowman-Birk Inhibitor (BBI) is an orally active serine protease inhibitor derived from soybeans. BBI concentrate (BBIC) is an extract of soybeans enriched in BBI...
  34. Robert P Schleimer; Fiscal Year: 2016
    ..that epithelial cells from individuals with CRS express dramatically lower levels of the barrier-preserving serine protease inhibitor SPINK5 and molecules in the epidermal differentiation complex (EDC), including S100A7 (psoriasin), ..
  35. Exploration of novel pan-HPV treatments to block development of AIDS-associated c
    Wijbe Martin Kast; Fiscal Year: 2010
    ..Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor found in mucosal fluids of the genital tract and has been shown to inhibit infection of macrophages ..
  36. Chen Yong Lin; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Matriptase is a serine protease that is expressed in the epithelial components of essentially all epithelial tissues including the mammary gland...
  37. A Role of Novel Serpin Maspinin Prostate Tumor/Bone Interaction
    Shijie Sheng; Fiscal Year: 2012
    ..Maspin is a novel epithelial-specific serine protease inhibitor (serpin)...
  38. Dynamic Networks and Mechanisms of Allosteric Communication in Proteins
    Andrew L Lee; Fiscal Year: 2012
    ..The serine protease inhibitor eglin c is a good example of this: conservative mutations in eglin c lead to long-range dynamic ..
  39. CHARLES SCOTT CRAIK; Fiscal Year: 2016
    ..All HHVs express a dimeric serine protease that is essential to the viral life cycle...
  40. Development of Antiviral Therapeutics for Dengue: Inhibitors of Viral Protease
    Radhakrishnan Padmanabhan; Fiscal Year: 2010
    ..approach for identification and further development of lead molecules already in hand against DENV serine protease (DENV NSSpro) and RNA-dependent RNA polymerase (DENV RdRP)...
  41. Developing the Synthetic Utility of the Copper-Catalyzed Diboration of Aldehydes
    Timothy B Clark; Fiscal Year: 2010
    ..One advantage of the proposed methodology is to provide access to new -aminoboronate esters and acids, known serine protease inhibitors and an essential component of Bortezomib (Velcade), an FDA-approved treatment of multiple myeloma...
  42. Barry M Trost; Fiscal Year: 2016
    ..The serine protease inhibitors, the aeruginosins, have potential as antiviral agents...
  43. Regulation of C. elegans Sperm Differentiation
    GILLIAN STANFIELD; Fiscal Year: 2012
    ..Our preliminary studies have identified a protease inhibitor, SWM-1, and serine protease, TRY-5, that appear to function antagonistically to regulate sperm activation in males...
  44. Ivory Dean; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Maspin is an epithelial-specific serine protease inhibitor (serpin) with tumor suppressive function. Exogenously added maspin inhibits tumor cell invasion and motility in vitro...
  45. Dengue and West Nile Viral Protease Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2005
    ..The two-component dengue viral serine protease, required in this process has been expressed in E...
  46. Hepcidin-based screening for infantile iron deficiency
    Mark D Fleming; Fiscal Year: 2010
    ..Recently, we identified biallelic mutations in the liver-specific transmembrane serine protease, TMPRSS6, in individuals with the phenotype opposite to hereditary hemochromatosis-a disorder termed iron ..
  47. CHIH CHANG KEVIN WEI; Fiscal Year: 2014
    ..effect, BK is chemotactic for inflammatory cells, in particular mast cells (MCs), which are the source of the serine protease chymase...
  48. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..NS3, in association with NS2B, is a serine protease that is required for polyprotein processing, a key early event in the virus life cycle...
  49. ELIZABETH ANN EKLUND; Fiscal Year: 2016
    ..We identified GAS2 as another relevant Icsbp-target-gene. Gas2 inhibits calpain;a serine protease with substrates that include bcate-nin, Stat3, Stat5 and Xiap...
  50. Mechanistic Characterization and Genetic Analysis of Systemic Iron Homeostasis
    Xin Du; Fiscal Year: 2011
    ..Mask phenotype was traced to a splicing error affecting the type II transmembrane serine protease 6 (TMPRSS6)...
  51. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..Of these effectors, the serine protease chlamydial protease-like activity factor (CPAF) is an enzyme that has emerged as a central virulence factor ..
  52. Prolylcarboxypeptidase is a Risk Factor for Cardiovascular Disease
    Alvin H Schmaier; Fiscal Year: 2013
    ..PRCP degrades angiotensin II (AngII) and bradykinin 1-8. We identified that the serine protease PRCP as a cell- surface plasma prekallikrein activator independent of factor XIIa...
  53. Clinical trial of self complementary AAV8-mediated gene transfer for hemophilia B
    Andrew M Davidoff; Fiscal Year: 2013
    ..X-linked recessive bleeding disorder that results from a defect in the factor IX (FIX) gene which encodes a serine protease critical for appropriate fibrin clot formation...
  54. Robert J Parmer; Fiscal Year: 2016
    ..Results have emerged that document a critical role for serine protease-mediated cleavage of ENaC subunits in ENaC activation, and recent studies suggest that plasmin is a key ..
  55. Inflammatory Proteases, Ubiquitin Proteasome System, and Myocyte Death
    Abdelkarim Sabri; Fiscal Year: 2012
    ..We have shown that the neutrophil derived serine protease, cathepsin G (CG), induces myocyte death by anoikis that involves downregulation of proteins that maintain ..
  56. Structure and function analysis of C1/C1q and MBL using a novel peptide inhibitor
    NEEL KUMAR KRISHNA; Fiscal Year: 2012
    ..1 will characterize the interactions between the CPP and C1q/MBL and measure functional effects on cognate serine protease activation by: (a) defining the interaction domains of CPP/C1q and CPP/MBL via binding (ELISA) assays, ..
  57. Effect of Macrolide Treatment on a Novel Pathway of Neutrophilic Inflammation in
    J Edwin Blalock; Fiscal Year: 2010
    ..process initially involving matrix metalloproteases (MMP)-8 and/or MMP-9 with prolyl endopeptidase (PE), a serine protease, catalyzing the final reaction...
  58. Francis J Castellino; Fiscal Year: 2015
    ..Through this overal mechanism, M and M-like proteins enhance the activation of Pg to the serine protease, plasmin (Pm), by bacterial or host Pg activators, thus creating a proteolytic bacterial microenvironment ..
  59. Nature of mamalian Cutaneous Permeablilty Barrier
    Peter M Elias; Fiscal Year: 2012
    ..enzyme Kallikrein 7 (Klk7) and loss-of-function mutations in SPINK5, which encodes a key epidermal serine protease (SP) inhibitor, lymphoepithelial Kazal-type trypsin inhibitor (LEKTI)...
  60. ELUCIDATING THE FUNCTION OF MATRIPTASE-ACTIVATED PDGF D IN BONE METASTATIC PROSTA
    ABDO JAMAL NAJY; Fiscal Year: 2012
    ..in epithelial cells as an inactive dimer and is proteolytically activated by the epithelial specific serine protease, matriptase...
  61. Qingyu Wu; Fiscal Year: 2015
    ..In this study, we will try to understand how corin works and if we can develop new corin-based methods to diagnose and treat high blood pressure during pregnancy. 1 ..
  62. Pneumococcal capsule and host innate immunity
    Moon H Nahm; Fiscal Year: 2012
    ..targets acetyl groups, and initiates the lectin-dependent complement cascade by activating MBL- associated serine protease-2 (MASP-2)...
  63. Ahsan Husain; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Chymase, a multifunctional mast cell serine protease, generates angiotensin (Ang) II and activates pro-matrix metalloproteinase (MMP)-2/9, in vivo, in the mouse heart...
  64. EXTRAVASCULAR THROMBIN REGULATION BY HEPARIN COFACTOR II
    FRANK CHURCH; Fiscal Year: 2004
    ..Our long-term goal is to understand the interactions between the "glycosaminoglycan (GAG)-dependent" serine protease inhibitor (serpin) heparin cofactor II (HCII), GAGs and thrombin and attempt to define how these interactions ..
  65. The Omi/HtrA2 Signal Transduction Pathway
    EMAD ALNEMRI; Fiscal Year: 2009
    ..Recent results from our lab and others suggested that the mitochondrial serine protease Omi/HtrA2 is a sensor of mitochondrial stress...
  66. Investigation of protein interactions in intrinsic blood coagulation pathway
    Divi Venkateswarlu; Fiscal Year: 2007
    ..Intrinsic pathway of blood coagulation involves the proteolytic activation of zymogenic factor X by serine protease IXa in the presence of phospholipids and divalent metal ions...