EPAS1

Summary

Gene Symbol: EPAS1
Description: endothelial PAS domain protein 1
Alias: ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-2-alpha, HIF2-alpha, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, hypoxia-inducible factor 2 alpha, member of PAS protein 2
Species: human

Top Publications

  1. pmc E2A-HLF-mediated cell transformation requires both the trans-activation domains of E2A and the leucine zipper dimerization domain of HLF
    T Yoshihara
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Mol Cell Biol 15:3247-55. 1995
  2. ncbi Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein
    M Ohh
    Department of Medicine, Brigham and Womens Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Cell Biol 2:423-7. 2000
  3. ncbi Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer
    M R Kelley
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 702 Barnhill Drive, Indianapolis, Indiana 46202, USA
    Clin Cancer Res 7:824-30. 2001
  4. pmc Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutations
    Lianjie Li
    Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Harvard Medical School, 44 Binney Street, Mayer 457, Boston, MA 02115, USA
    Mol Cell Biol 27:5381-92. 2007
  5. doi Assessment of intestinal absorption of vitexin-2''-o-rhamnoside in hawthorn leaves flavonoids in rat using in situ and in vitro absorption models
    Ying ai Xu
    Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, PR China
    Drug Dev Ind Pharm 34:164-70. 2008
  6. ncbi Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensor
    Jens Köditz
    Department of Heart and Circulatory Physiology, Center of Physiology and Pathophysiology, Georg August University Gottingen, Gottingen, Germany
    Blood 110:3610-7. 2007
  7. pmc The peptidyl prolyl cis/trans isomerase FKBP38 determines hypoxia-inducible transcription factor prolyl-4-hydroxylase PHD2 protein stability
    Sandra Barth
    Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Mol Cell Biol 27:3758-68. 2007
  8. doi Prolyl-4-hydroxylase PHD2- and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesis
    M R Bordoli
    Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland
    Oncogene 30:548-60. 2011
  9. ncbi Thymosin β4 expression correlates with lymph node metastasis through hypoxia inducible factor-α induction in breast cancer
    Sun Young Yoon
    Department of Life Science, Sookmyung Women s University, Yongsan gu, Seoul, Republic of Korea
    Oncol Rep 25:23-31. 2011
  10. ncbi Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells
    H Tian
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Genes Dev 11:72-82. 1997

Research Grants

  1. Frank S Lee; Fiscal Year: 2014
  2. Yatrik M Shah; Fiscal Year: 2016
  3. THERAPY FOR GASTROINTESTINAL DISORDERS
    Elizabeth Dial; Fiscal Year: 1999
  4. Robert G Roeder; Fiscal Year: 2016
  5. Christopher A Pennell; Fiscal Year: 2015
  6. Mechanism of Redox Regulation by Ape1/Ref-1
    Millie M Georgiadis; Fiscal Year: 2010
  7. Hamid M Said; Fiscal Year: 2016
  8. Karen M Lyons; Fiscal Year: 2015
  9. Analysis of Cell Death Regulation in C. elegans
    Barbara Conradt; Fiscal Year: 2012
  10. E Jason Abel; Fiscal Year: 2016

Detail Information

Publications209 found, 100 shown here

  1. pmc E2A-HLF-mediated cell transformation requires both the trans-activation domains of E2A and the leucine zipper dimerization domain of HLF
    T Yoshihara
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Mol Cell Biol 15:3247-55. 1995
    The E2A-HLF fusion gene, formed by the t(17;19)(q22;p13) translocation in childhood acute pro-B-cell leukemia, encodes a hybrid protein that contains the paired trans-activation domains of E2A (E12/E47) linked to the basic region/leucine ..
  2. ncbi Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein
    M Ohh
    Department of Medicine, Brigham and Womens Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Cell Biol 2:423-7. 2000
    ..These results strengthen the link between overaccumulation of HIF and development of VHL disease...
  3. ncbi Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer
    M R Kelley
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 702 Barnhill Drive, Indianapolis, Indiana 46202, USA
    Clin Cancer Res 7:824-30. 2001
    ..box containing genes (PAX), nuclear factor-kappaB, hypoxia-inducible factor alpha (HIF-1alpha), HIF-like factor (HLF), p53, and others. The expression of Ape1/ref-1 in prostate has not been characterized previously...
  4. pmc Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutations
    Lianjie Li
    Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Harvard Medical School, 44 Binney Street, Mayer 457, Boston, MA 02115, USA
    Mol Cell Biol 27:5381-92. 2007
    ..Therefore, quantitative differences with respect to HIF deregulation are sufficient to account for the differential risks of kidney cancer linked to VHL mutations...
  5. doi Assessment of intestinal absorption of vitexin-2''-o-rhamnoside in hawthorn leaves flavonoids in rat using in situ and in vitro absorption models
    Ying ai Xu
    Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, PR China
    Drug Dev Ind Pharm 34:164-70. 2008
    ..the absorption mechanism of vitexin-2''-O-rhamnoside, the index component in hawthorn leaves flavonoids (HLF) in the rat intestine, using two different absorption models, the in situ single-pass intestinal perfusion and the ..
  6. ncbi Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensor
    Jens Köditz
    Department of Heart and Circulatory Physiology, Center of Physiology and Pathophysiology, Georg August University Gottingen, Gottingen, Germany
    Blood 110:3610-7. 2007
    ..These data demonstrate that PHD-dependent oxygen-sensing recruits both the hypoxia-inducible factor (HIF) and ATF-4 systems, and hence not only confers adaptive responses but also cell fate decisions...
  7. pmc The peptidyl prolyl cis/trans isomerase FKBP38 determines hypoxia-inducible transcription factor prolyl-4-hydroxylase PHD2 protein stability
    Sandra Barth
    Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Mol Cell Biol 27:3758-68. 2007
    ..Downregulation of FKBP38 did not affect PHD2 mRNA levels but prolonged PHD2 protein stability, suggesting that FKBP38 is involved in PHD2 protein regulation...
  8. doi Prolyl-4-hydroxylase PHD2- and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesis
    M R Bordoli
    Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland
    Oncogene 30:548-60. 2011
    ..Our data suggest that PHD2/HIF-2/amphiregulin signaling has a critical role in the regulation of breast tumor progression and propose PHD2 as a potential tumor suppressor in breast cancer...
  9. ncbi Thymosin β4 expression correlates with lymph node metastasis through hypoxia inducible factor-α induction in breast cancer
    Sun Young Yoon
    Department of Life Science, Sookmyung Women s University, Yongsan gu, Seoul, Republic of Korea
    Oncol Rep 25:23-31. 2011
    ..Ultimately, these results highlight Tβ4 as a potentially therapeutic target in malignant cancers...
  10. ncbi Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells
    H Tian
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Genes Dev 11:72-82. 1997
    Here we describe the cloning and characterization of a PAS domain transcription factor termed endothelial PAS-1 (EPAS1)...
  11. ncbi Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway
    J B Hogenesch
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Biol Chem 272:8581-93. 1997
    ..studies, yeast two-hybrid analysis, and transient transfection experiments demonstrated that MOP1 and MOP2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo...
  12. pmc A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1alpha regulates the VEGF expression and is potentially involved in lung and vascular development
    M Ema
    Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980 77, Japan
    Proc Natl Acad Sci U S A 94:4273-8. 1997
    ..The HIF1alpha-like factor (HLF) encoded by the isolated cDNA bound the hypoxia-response element (HRE) found in enhancers of genes for ..
  13. ncbi The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
    P H Maxwell
    Wellcome Trust Centre for Human Genetics, Oxford, UK
    Nature 399:271-5. 1999
    ..Thus, constitutive HIF-1 activation may underlie the angiogenic phenotype of VHL-associated tumours. The pVHL/HIF-1 interaction provides a new focus for understanding cellular oxygen sensing...
  14. ncbi Hypoxia inducible factor-alpha binding and ubiquitylation by the von Hippel-Lindau tumor suppressor protein
    M E Cockman
    Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom
    J Biol Chem 275:25733-41. 2000
    ....
  15. ncbi HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing
    M Ivan
    Dana Farber Cancer Institute and Brigham and Women s Hospital, Howard Hughes Medical Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
    Science 292:464-8. 2001
    ..Because proline hydroxylation requires molecular oxygen and Fe(2+), this protein modification may play a key role in mammalian oxygen sensing...
  16. ncbi Contrasting effects on HIF-1alpha regulation by disease-causing pVHL mutations correlate with patterns of tumourigenesis in von Hippel-Lindau disease
    S C Clifford
    Section of Medical and Molecular Genetics, Department of Paediatrics and Child Health, University of Birmingham, The Medical School, Edgbaston, Birmingham B15 2TT, UK
    Hum Mol Genet 10:1029-38. 2001
    ..Our findings are consistent with impaired ability to degrade HIF-alpha subunit being required for HAB development and RCC susceptibility...
  17. ncbi Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch
    David Lando
    Department of Molecular Biosciences Biochemistry, Adelaide University, SA 5005, Australia
    Science 295:858-61. 2002
    ..Full induction of HIF-1alpha and -2alpha, therefore, relies on the abrogation of both Pro and Asn hydroxylation, which during normoxia occur at the degradation and COOH-terminal transactivation domains, respectively...
  18. ncbi Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)
    Gerd Elvert
    Zentrum für Molekulare Medizin der Universität zu Köln, Joseph Stelzmann Strasse 9, 50931 Koln, Germany
    J Biol Chem 278:7520-30. 2003
    ....
  19. ncbi Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein
    Paul G Corn
    Department of Medicine, Howard Hughes Medical Institute and Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Genet 35:229-37. 2003
    ..Thus, the ability of pVHL to degrade Hif1 alpha depends in part on its interaction with TBP-1 and suggests a new mechanism for Hif1 alpha stabilization in some pVHL-deficient tumors...
  20. pmc Differential roles of hypoxia-inducible factor 1alpha (HIF-1alpha) and HIF-2alpha in hypoxic gene regulation
    Cheng Jun Hu
    Abramson Family Cancer Research Institute Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    Mol Cell Biol 23:9361-74. 2003
    ..Importantly, HIF-1alpha (and not HIF-2alpha) stimulates glycolytic gene expression in both types of cells, clearly showing for the first time that HIF-1alpha and HIF-2alpha have unique targets...
  21. pmc Inhibition of HIF2alpha is sufficient to suppress pVHL-defective tumor growth
    Keiichi Kondo
    Department of Adult Oncology, Dana Farber Cancer Institute and Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 1:E83. 2003
    ..These results establish that tumor suppression by pVHL is linked to regulation of HIF target genes...
  22. ncbi Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alpha
    Tokujiro Uchida
    Department of Physiology, INSERM U426, Faculte de Medecine Xavier Bichat, Universite Paris 7, 75870 Paris, Cedex 18, France
    J Biol Chem 279:14871-8. 2004
    ..This trans-regulation between HIF-1alpha and HIF-2alpha during hypoxia likely conveys target gene specificity...
  23. ncbi Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumors
    Michael Zimmer
    Hematology Oncology Unit, Department of Medicine, Massachusetts General Hospital and the Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA
    Mol Cancer Res 2:89-95. 2004
    ..These data suggest that deregulation of hypoxia-inducible genes in VHL-/- cells can be attributed mainly to deregulation of HIF and validate HIF as a therapeutic anticancer drug target...
  24. ncbi Differentiating the functional role of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha (EPAS-1) by the use of RNA interference: erythropoietin is a HIF-2alpha target gene in Hep3B and Kelly cells
    Christina Warnecke
    Department of Nephrology and Medical Intensive Care, Virchow Clinic, Charite University Medicine, Berlin, Germany
    FASEB J 18:1462-4. 2004
    ..In conclusion, we found a remarkably restricted target gene specificity of the HIFalpha subunits, which can be overcome in cells with perturbations in the pVHL/HIF system and under forced expression...
  25. ncbi Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor
    Rebecca J Appelhoff
    Henry Wellcome Bldg of Genomic Medicine, Roosevelt Drive, Headington, Oxford, UK
    J Biol Chem 279:38458-65. 2004
    ....
  26. ncbi Activity of hypoxia-inducible factor 2alpha is regulated by association with the NF-kappaB essential modulator
    Cameron P Bracken
    School of Molecular and Biomedical Science and the Centre for the Molecular Genetics of Development, University of Adelaide, Adelaide, South Australia, 5005, Australia
    J Biol Chem 280:14240-51. 2005
    ....
  27. pmc Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinoma
    Raju R Raval
    The Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford OX3 7BN, United Kingdom
    Mol Cell Biol 25:5675-86. 2005
    ..These findings indicate that therapeutic approaches to targeting of the HIF system, at least in this setting, will need to take account of HIF isoform-specific functions...
  28. ncbi The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletes
    Jennifer Henderson
    Department of Molecular and Clinical Genetics, Royal Prince Alfred Hospital and Central Clinical School, The University of Sydney K25, Camperdown, 2050, Australia
    Hum Genet 118:416-23. 2005
    b>EPAS1 is a gene involved in complex oxygen sensing. It is expressed in microvascular endothelial cells, lung epithelial cells, cardiac myocytes and the brain...
  29. pmc A family with erythrocytosis establishes a role for prolyl hydroxylase domain protein 2 in oxygen homeostasis
    Melanie J Percy
    Department of Haematology, Belfast City Hospital, Belfast BT9 7AB, Northern Ireland, United Kingdom
    Proc Natl Acad Sci U S A 103:654-9. 2006
    ..Our findings indicate that PHD2 is critical for normal regulation of HIF in humans...
  30. ncbi Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment
    Cameron P Bracken
    School of Molecular and Biomedical Science, University of Adelaide, South Australia
    J Biol Chem 281:22575-85. 2006
    ..Interestingly, the initial stabilization of HIF-alpha prior to transactivation up-regulation predicted from in vitro derived hydroxylation data is only true for a subset of cells...
  31. ncbi Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha
    Toshiaki Shinojima
    Department of Urology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    Carcinogenesis 28:529-36. 2007
    ..HIF-1alpha may therefore represent an important target molecule for RCC therapy; however, HIF-2alpha should be targeted in HIF-1alpha defective renal cancer cells...
  32. ncbi Recruitment of HIF-1alpha and HIF-2alpha to common target genes is differentially regulated in neuroblastoma: HIF-2alpha promotes an aggressive phenotype
    Linda Holmquist-Mengelbier
    Division of Molecular Medicine, Department of Laboratory Medicine, Lund University, University Hospital MAS, SE 205 02 Malmo, Sweden
    Cancer Cell 10:413-23. 2006
    ..Our results demonstrate the relevance of HIF-2alpha in neuroblastoma progression and have general tumor biological implications...
  33. ncbi Iron-regulatory proteins limit hypoxia-inducible factor-2alpha expression in iron deficiency
    Mayka Sanchez
    Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
    Nat Struct Mol Biol 14:420-6. 2007
    ..element (IRE) in the 5' untranslated region of the messenger RNA encoding endothelial PAS domain protein-1, EPAS1 (also called hypoxia-inducible factor-2alpha, HIF2alpha)...
  34. pmc HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity
    John D Gordan
    Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    Cancer Cell 11:335-47. 2007
    ..Enhanced c-Myc activity likely contributes to HIF-2alpha-mediated neoplastic progression following loss of the VHL tumor suppressor and influences the behavior of hypoxic tumor cells...
  35. pmc Stromal expression of hypoxia regulated proteins is an adverse prognostic factor in colorectal carcinomas
    Arjen H G Cleven
    Departments of Pathology, GROW Research Institute, University Hospital Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands
    Cell Oncol 29:229-40. 2007
    ..We studied the role of protein expression of hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, carbonic anhydrase 9 (CA9) and glucose transporter 1 (GLUT1) in patients with colorectal adenocarcinomas...
  36. pmc Novel exon 12 mutations in the HIF2A gene associated with erythrocytosis
    Melanie J Percy
    Department of Haematology, Belfast City Hospital, Belfast, United Kingdom
    Blood 111:5400-2. 2008
    ..This raises the possibility of HIF2A mutations being associated with other cases of erythrocytosis...
  37. doi The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors
    Christina Warnecke
    Department of Nephrology and Hypertension, University Clinic Erlangen, Germany
    Exp Cell Res 314:2016-27. 2008
    ..Thus, HIF-2alpha effects are modulated by a number of intrinsic and extrinsic factors which may be most relevant in tumour cells...
  38. doi Autosomal dominant erythrocytosis and pulmonary arterial hypertension associated with an activating HIF2 alpha mutation
    Daniel P Gale
    Blood 112:919-21. 2008
  39. pmc Familial erythrocytosis arising from a gain-of-function mutation in the HIF2A gene of the oxygen sensing pathway
    Melanie J Percy
    Department of Haematology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK
    Ulster Med J 77:86-8. 2008
    ..Continued study of idiopathic cases of erythrocytosis with raised serum Epo will reveal whether HIF-2 alpha will be a major cause of erythrocytosis on par with VHL...
  40. pmc Differential dependence of hypoxia-inducible factors 1 alpha and 2 alpha on mTORC1 and mTORC2
    Alfredo Toschi
    Department of Biological Sciences, Hunter College, The City University of New York, New York, New York 10021, USA
    J Biol Chem 283:34495-9. 2008
    ..Constitutive expression of HIF1 alpha and HIF2 alpha has been linked to a number of human cancers, especially renal cell carcinoma (RCC), in which HIF2 alpha ..
  41. pmc HIF-1alpha and HIF-2alpha have divergent roles in colon cancer
    Takaaki Imamura
    Gastrointestinal Unit, Massachusetts General Hospital, Boston, MA 02114, USA
    Int J Cancer 124:763-71. 2009
    ..In particular, HIF-1alpha promoted the growth of SW480 colon cancer cells but HIF-2alpha appeared to restrain growth. Consequently, therapeutic approaches that target HIF may need to consider these isoform-specific properties...
  42. pmc Hypoxia-inducible factor-1alpha and -2alpha are expressed in most rectal cancers but only hypoxia-inducible factor-1alpha is associated with prognosis
    S Rasheed
    Department of Surgery, St Mark s Hospital, Harrow, Middlesex, UK
    Br J Cancer 100:1666-73. 2009
    ..Our results indicate that HIF-1alpha, but not HIF-2alpha, might be used as a marker of prognosis, in addition to methods currently used, to enhance patient management...
  43. doi Regulation of hypoxia-inducible factor 2alpha signaling by the stress-responsive deacetylase sirtuin 1
    Elhadji M Dioum
    Veterans Affairs North Texas Health Care System, Department of Medicine, 4500 South Lancaster Road, Dallas, TX 75216, USA
    Science 324:1289-93. 2009
    ..Thus, Sirt1 promotes HIF-2 signaling during hypoxia and likely other environmental stresses...
  44. pmc HIF2alpha inhibition promotes p53 pathway activity, tumor cell death, and radiation responses
    Jessica A Bertout
    Abramson Family Cancer Research Institute, Abramson Cancer Center, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 106:14391-6. 2009
    ..These results correlate with altered p53 phosphorylation and target gene expression in untreated human tumor samples and show that HIF2alpha likely contributes to tumor cell survival including during radiation therapy...
  45. pmc Human cancers converge at the HIF-2alpha oncogenic axis
    Aleksandra Franovic
    Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
    Proc Natl Acad Sci U S A 106:21306-11. 2009
    ....
  46. doi Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factors
    Anne Theres Henze
    Institute of Neuropathology, Giessen, Germany
    Cancer Res 70:357-66. 2010
    ....
  47. pmc The NADPH oxidase subunit p22phox inhibits the function of the tumor suppressor protein tuberin
    Karen Block
    Department of Medicine, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    Am J Pathol 176:2447-55. 2010
    ..Our data provide the first evidence that p22(phox)-based Nox oxidases maintain HIF-2alpha protein expression through inactivation of tuberin and downstream activation of ribosomal protein S6 kinase 1/4E-BP1 pathway...
  48. pmc Natural selection on EPAS1 (HIF2alpha) associated with low hemoglobin concentration in Tibetan highlanders
    Cynthia M Beall
    Department of Anthropology, Case Western Reserve University, Cleveland, OH 44106 7125, USA
    Proc Natl Acad Sci U S A 107:11459-64. 2010
    ..m) with closely related lowland Han revealed a genome-wide significant divergence across eight SNPs located near EPAS1. This gene encodes the transcription factor HIF2alpha, which stimulates production of red blood cells and thus ..
  49. pmc Sequencing of 50 human exomes reveals adaptation to high altitude
    Xin Yi
    BGI Shenzhen, Shenzhen 518083, China
    Science 329:75-8. 2010
    ..The strongest signal of natural selection came from endothelial Per-Arnt-Sim (PAS) domain protein 1 (EPAS1), a transcription factor involved in response to hypoxia...
  50. doi A genome-wide search for signals of high-altitude adaptation in Tibetans
    Shuhua Xu
    Chinese Academy of Sciences and Max Planck Society Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Mol Biol Evol 28:1003-11. 2011
    ..2 and 2p21. EGLN1 (or HIFPH2, MIM 606425) and EPAS1 (or HIF2A, MIM 603349), both related to hypoxia-inducible factor, were found most differentiated in the two ..
  51. pmc Hypoxia-inducible factors and the response to hypoxic stress
    Amar J Majmundar
    Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell 40:294-309. 2010
    ..These transcription factors function in normal tissue homeostasis and impinge on critical aspects of disease progression and recovery. Insights from basic HIF biology are being translated into pharmaceuticals targeting the HIF pathway...
  52. doi Genetic variations in Tibetan populations and high-altitude adaptation at the Himalayas
    Yi Peng
    State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, China
    Mol Biol Evol 28:1075-81. 2011
    ..combination with the reported data, we identified strong signals of selective sweep in two hypoxia-related genes, EPAS1 and EGLN1...
  53. pmc Cardiopulmonary function in two human disorders of the hypoxia-inducible factor (HIF) pathway: von Hippel-Lindau disease and HIF-2alpha gain-of-function mutation
    Federico Formenti
    Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Rd, Oxford, OX1 3PT, UK
    FASEB J 25:2001-11. 2011
    ....
  54. pmc Opposite effects of HIF-1α and HIF-2α on the regulation of IL-8 expression in endothelial cells
    Urszula Florczyk
    Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
    Free Radic Biol Med 51:1882-92. 2011
    ..However, despite stabilization of both isoforms in hypoxia the effect of HIF-1 is predominant, and downregulation of IL-8 expression in hypoxia is caused by attenuation of Nrf2 and c-Myc...
  55. pmc HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells
    Shiro Koizume
    Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
    Nucleic Acids Res 40:5389-401. 2012
    ..These results suggest the presence of a stress-responsive transcription mediated by the HIF2α/Sp1/HDAC4 network and explain how CCC shed their procoagulant activity under hypoxia...
  56. pmc The acetylase/deacetylase couple CREB-binding protein/Sirtuin 1 controls hypoxia-inducible factor 2 signaling
    Rui Chen
    Department of Medicine, Veterans Affairs North Texas Health Care System, Dallas, TX 75216, USA
    J Biol Chem 287:30800-11. 2012
    ..Maximal CBP/HIF-2 signaling requires intact CBP acetyltransferase activity in both Hep3B cells as well as in mice...
  57. pmc Somatic HIF2A gain-of-function mutations in paraganglioma with polycythemia
    Zhengping Zhuang
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    N Engl J Med 367:922-30. 2012
    ..Here we report two novel somatic gain-of-function mutations in the gene encoding hypoxia-inducible factor 2α (HIF2A) in two patients, one presenting with paraganglioma and the other with paraganglioma and somatostatinoma, both of ..
  58. pmc Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas
    Chunzhang Yang
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20894, USA
    Blood 121:2563-6. 2013
    ..Here, we report 2 additional unique HIF2A mutations, which disrupt the hydroxylation domain recognized by prolyl hydroxylase domain-2 containing protein, ..
  59. ncbi Regulation of HIF prolyl hydroxylases by hypoxia-inducible factors
    Olga Aprelikova
    Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Cell Biochem 92:491-501. 2004
    ..These data further advance our understanding of the differential role of HIF factors and putative feedback loop in HIF regulation...
  60. doi Hypoxia-inducible factor-2alpha correlates to distant recurrence and poor outcome in invasive breast cancer
    Karolina Helczynska
    Center for Molecular Pathology, Department of Laboratory Medicine, University Hospital MAS, Malmo, Sweden
    Cancer Res 68:9212-20. 2008
    ..At prolonged hypoxia, HIF-2alpha remained accumulated, whereas HIF-1alpha protein levels decreased, in agreement with the oxygen level and time-dependent induction of HIFs recently reported in neuroblastoma...
  61. ncbi Determination and comparison of specific activity of the HIF-prolyl hydroxylases
    Jason R Tuckerman
    Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN, UK
    FEBS Lett 576:145-50. 2004
    ....
  62. ncbi The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases
    Peppi Koivunen
    Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014, Oulu, Finland
    J Biol Chem 281:28712-20. 2006
    ..Even this value is high compared with tissue O(2) levels, indicating that HIF-P4Hs are effective oxygen sensors...
  63. pmc Tumor cell invasion of von Hippel Lindau renal cell carcinoma cells is mediated by membrane type-1 matrix metalloproteinase
    Brenda L Petrella
    Department of Medicine, Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, NH, USA
    Mol Cancer 5:66. 2006
    ..The aim of this study was to investigate the contribution of MT1-MMP to the invasive potential of RCC cells using in vitro type I collagen degradation and invasion assays...
  64. doi A novel heterozygous HIF2AM535I mutation reinforces the role of oxygen sensing pathway disturbances in the pathogenesis of familial erythrocytosis
    Maurizio Martini
    Department of Pathology, Catholic University, Rome, Italy
    Haematologica 93:1068-71. 2008
    b>HIF2A transcription factor plays a central role in the regulation of the hypoxia responding pathway in mammalian cells, by modulating erythropoiesis and angiogenesis...
  65. doi Heterogeneity in binding and gene-expression regulation by HIF-2alpha
    Shiro Koizume
    Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
    Biochem Biophys Res Commun 371:251-5. 2008
    ..These results demonstrate that, unlike HIF-1alpha, HIF-2alpha differentially binds and regulates transcription under hypoxia...
  66. ncbi Clock genes in the heart: characterization and attenuation with hypertrophy
    M E Young
    Department of Internal Medicine, Division of Cardiology, University of Texas-Houston Medical School, Houston, TX 77030, USA
    Circ Res 88:1142-50. 2001
    ..heart all mammalian homologues of known Drosophila clock genes (bmal1, clock, cry1, cry2, per1, per2, per3, dbp, hlf, and tef) show circadian patterns of expression and that the induction of clock output genes (the PAR [rich in ..
  67. ncbi One hundred sixteen cases of acute liver failure treated with MARS
    G Novelli
    Policlinico Umberto I, University of Rome La Sapienza, Rome, Italy
    Transplant Proc 37:2557-9. 2005
    ..The mean length of application was about 9 hours (range: 8-20). MARS treatment was carried out in HLF patients with continuous acute-on-chronic hepatic failure dialisate flow similar to continuous veno venus ..
  68. ncbi PDP1, a novel Drosophila PAR domain bZIP transcription factor expressed in developing mesoderm, endoderm and ectoderm, is a transcriptional regulator of somatic muscle genes
    S C Lin
    Department of Biochemistry and Molecular Biology M C536, University of Illinois College of Medicine, Chicago 60612, USA
    Development 124:4685-96. 1997
    ..a novel transcription factor that is highly homologous to the PAR subfamily of mammalian bZIP transcription factors HLF, DBP and VBP/TEF...
  69. ncbi Thoracic myelopathy caused by ossification of the ligamentum flavum. Clinicopathologic study and surgical treatment
    K Okada
    Department of Orthopaedic Surgery, Kagawa Medical School, Japan
    Spine (Phila Pa 1976) 16:280-7. 1991
    ..cells were found in the increased and swollen collagen fibers forming the hypertrophic ligamentum flavum (HLF). Ossification extended along the superficial layer of HLF...
  70. pmc Inhibitory effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermedia
    Hiroyuki Wakabayashi
    Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, 5 1 83 Higashihara, Zama, Kanagawa 228 8583, Japan
    Antimicrob Agents Chemother 53:3308-16. 2009
    ..gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >or=130 microg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >or=6 microg/ml of bLF-..
  71. doi Human lactoferrin activates NF-kappaB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide-stimulated TLR4 signaling
    Ken Ando
    Tokyo University of Pharmacy and Life Sciences, Japan
    FEBS J 277:2051-66. 2010
    ..Here we reveal the signal transduction pathway responsible for human LF (hLF)-triggered nuclear factor-kappaB (NF-kappaB) activation...
  72. ncbi Variation in antimicrobial activity of lactoferricin-derived peptides explained by structure modelling
    Sebastien Farnaud
    Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, St Thomas Street, London SE1 1UL, UK
    FEMS Microbiol Lett 238:221-6. 2004
    ..differences observed between the bovine lactoferrin (BLf) and LfcinB were also found between the human lactoferrin (HLf) and the LfcinH...
  73. pmc Antimicrobial mechanism of action of transferrins: selective inhibition of H+-ATPase
    María T Andrés
    Laboratory of Oral Microbiology, School of Stomatology, Oviedo, Spain
    Antimicrob Agents Chemother 54:4335-42. 2010
    ..In anaerobiosis, P. aeruginosa cells were not susceptible to lactoferrin (hLf) or transferrin (hTf)...
  74. ncbi Intravenous infusion of erythromycin inhibits CXC chemokine production, but augments neutrophil degranulation in whole blood stimulated with Streptococcus pneumoniae
    M J Schultz
    Laboratory of Experimental Internal Medicine, Department of Intensive Care, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
    J Antimicrob Chemother 46:235-40. 2000
    ..products bactericidal/permeability increasing protein (BPI), human neutrophil elastase (HNE) and human lactoferrin (HLF) upon stimulation with S. pneumoniae...
  75. doi Hispanic Labor Friends Initiative: supporting vulnerable women
    Cambria Jones Hazard
    College of Nursing, Brigham Young University, Provo, UT, USA
    MCN Am J Matern Child Nurs 34:115-21. 2009
    ..To evaluate the qualitative aspects of the Hispanic Labor Friends Initiative...
  76. doi Effects of Echinostoma caproni infection on the neutral and polar lipids of intestinal and non-intestinal organs in the BALB/c mouse as determined by high-performance thin-layer chromatography
    Jessica Counihan
    Department of Chemistry, Lafayette College, Easton, PA 18042, USA
    Parasitol Res 107:947-53. 2010
    ..Lipids were determined by high-performance thin-layer chromatography (HPTLC) with Analtech 10 x 20 cm HPTLC-HLF silica gel plates...
  77. doi Comprehensive characterization of the site-specific N-glycosylation of wild-type and recombinant human lactoferrin expressed in the milk of transgenic cloned cattle
    Tian Yu
    State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People s Republic of China
    Glycobiology 21:206-24. 2011
    ..Here we determined the site-specific N-glycosylation profile of human lactoferrin (hLF) and recombinant human lactoferrin (rhLF) expressed in the milk of transgenic cloned cattle...
  78. ncbi Recombinant human lactoferrin treatment for global health issues: iron deficiency and acute diarrhea
    Delia R Bethell
    Ventria Bioscience, 4110 N Freeway Boulevard, Sacramento, California 95834, USA
    Biometals 17:337-42. 2004
    ..of the diarrheal associated organisms: rotavirus, ETEC, cholera, salmonella, and shigella by human lactoferrin (hLF) and human lysozyme...
  79. doi Inhibition of poly adenosine diphosphate-ribose polymerase decreases hepatocellular carcinoma growth by modulation of tumor-related gene expression
    Rosa Quiles-Perez
    San Cecilio University Hospital, Granada, Spain
    Hepatology 51:255-66. 2010
    ..growth factor receptor-1, VEGFR1], epidermal growth factor receptor [EPAS1]/hypoxia-inducible factor 2 [HIF2A], EGLN1 [PHD2], epidermal growth factor receptor [EGFR], MYC, JUND, SPP1 [OPN], hepatocyte growth factor [HGF]) ..
  80. ncbi HIF-2alpha regulates murine hematopoietic development in an erythropoietin-dependent manner
    Marzia Scortegagna
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8573, USA
    Blood 105:3133-40. 2005
    ..We previously reported that mice lacking wild-type HIF-2alpha, encoded by the EPAS1 gene, exhibit pancytopenia. In this study, we have characterized the etiology of this hematopoietic phenotype...
  81. ncbi [Mammary gland dual-expression construct containing prolactin and foreign DNAs can promote the expression of foreign proteins in mammary cells]
    Feng Tao Liu
    Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200 040, China
    Yi Chuan 26:567-73. 2004
    To investigate the feasibility in the promotion of the expression of foreign proteins, such as human lactinferrin (hLF) and thrombopoietin (TPO), in the transgenic animals-mammary gland bioreactor by bovine prolactin (bPRL), two types of ..
  82. pmc Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes
    S Ikushima
    Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 94:2609-14. 1997
    The E2A-HLF (hepatic leukemia factor) oncoprotein, generated in pro-B lymphocytes by fusion of the trans-activation domain of E2A to the basic region/leucine zipper (bZIP) domain of HLF, functions as an anti-apoptotic transcription ..
  83. ncbi Environmental impacts on offspring survival during the lambing period in central Patagonia
    F Coronato
    Centro Nacional Patagónico CONICET, Puerto Madryn, Argentina
    Int J Biometeorol 43:113-8. 1999
    ..2-week averages of temperature (Tf), wind speed (Vf) and total precipitation (Pf), the average heat loss in sheep (HLf) was 40.40 - 2.07Tf + 5.64Vf + 0.04Pf. (r2 = 0.95)...
  84. ncbi 99mTc-Labeled UBI 29-41 peptide for monitoring the efficacy of antibacterial agents in mice infected with Staphylococcus aureus
    Peter H Nibbering
    Department of Infectious Diseases, Leiden University Medical Center C5 P, PO Box 9600, 2300 RC Leiden, The Netherlands
    J Nucl Med 45:321-6. 2004
    ....
  85. pmc Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAs
    Nikolay G Kolev
    Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06536, USA
    Genes Dev 19:2583-92. 2005
    ..cell extracts requires the U7 small nuclear ribonucleoprotein (U7 snRNP) and an unidentified heat-labile factor (HLF)...
  86. pmc The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice
    Michele M Hickey
    Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    J Clin Invest 120:827-39. 2010
    ..HIF-2alpha activity was increased in lungs from VhlR/R mice, and heterozygosity for Hif2a, but not Hif1a, genetically suppressed both the polycythemia and pulmonary hypertension in the VhlR/R mice...
  87. ncbi Effect of carrier fluid viscosity on retention time and resolution in gravitational field-flow fractionation
    Seungho Lee
    Department of Chemistry, Hannam University, Daejeon, South Korea
    Anal Chem 83:3343-51. 2011
    ..focused layers in the channel thickness where force due to gravity is exactly opposed by hydrodynamic lift forces (HLF)...
  88. ncbi Different elution modes and field programming in gravitational field-flow fractionation: field programming using density and viscosity gradients
    Jana Plocková
    Institute of Analytical Chemistry, AS CR, 611 42 Brno, Czech Republic
    J Chromatogr A 1118:253-60. 2006
    ..e. factors influencing hydrodynamic lift forces (HLF)...
  89. pmc Mycoplasma fermentans and TNF-beta interact to amplify immune-modulating cytokines in human lung fibroblasts
    James P Fabisiak
    Department of Environmental and Occupational Health, Center for Free Radical and Antioxidant Health, University of Pittsburgh Graduate School of Public Health, College of Medicine, Pittsburgh, PA 15219 3130, USA
    Am J Physiol Lung Cell Mol Physiol 291:L781-93. 2006
    ..Human lung fibroblasts (HLF) were exposed to live M...
  90. doi Fine mapping of a major QTL influencing morphine preference in C57BL/6 and DBA/2 mice using congenic strains
    Glenn A Doyle
    Department of Psychiatry, Center for Neurobiology and Behavior, University of Pennsylvania, Philadelphia, PA 19104, USA
    Neuropsychopharmacology 33:2801-9. 2008
    ..Here, we report the refinement of the Chr. 10 QTL in subcongenic strains of D2.B6-Mop2 congenic mice described previously...
  91. pmc DNA-binding specificity of the PAR basic leucine zipper protein VBP partially overlaps those of the C/EBP and CREB/ATF families and is influenced by domains that flank the core basic region
    N B Haas
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Mol Cell Biol 15:1923-32. 1995
    The PAR subfamily of basic leucine zipper (bZIP) factors comprises three proteins (VBP/TEF, DBP, and HLF) that have conserved basic regions flanked by proline- and acidic-amino-acid-rich (PAR) domains and functionally compatible leucine ..
  92. pmc DNA-binding and transcriptional regulatory properties of hepatic leukemia factor (HLF) and the t(17;19) acute lymphoblastic leukemia chimera E2A-HLF
    S P Hunger
    Department of Pathology, Stanford University School of Medicine, California 94305
    Mol Cell Biol 14:5986-96. 1994
    The t(17;19) translocation in acute lymphoblastic leukemias results in creation of E2A-hepatic leukemia factor (HLF) chimeric proteins that contain the DNA-binding and protein dimerization domains of the basic leucine zipper (bZIP) ..
  93. ncbi Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophages
    Delphine Sylvie Anne Beeckman
    Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium
    Vet Res 38:729-39. 2007
    The effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular pathogen Chlamydophila (Cp...
  94. ncbi Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions
    Donatella Aldinucci
    Experimental Oncology 2, Centro di Riferimento Oncologico, I R C C S, Aviano PN, Italy
    Int J Cancer 122:769-76. 2008
    ..CD40 engagement and also by coculturing L-1236 cells on primary stromal fibroblasts from HL-involved lymph nodes (HLF). Coculture experiments indicated that a direct contact of H-RS cells induces HLF cells to produce CCL5...
  95. ncbi The growth inhibition of hepatocellular and cholangiocellular carcinoma cells by gemcitabine and the roles of extracellular signal-regulated and checkpoint kinases
    Kazuya Matsumoto
    Second Department of Internal Medicine, Tottori University School of Medicine, Tottori, Japan
    Oncol Rep 20:863-72. 2008
    ..After HCC cells (HepG2, Hep3B, HLF and PLC/PRF/5) and CCC cells (HuCCT-1) were treated with gemcitabine, cellular growth, cell cycle, nuclear ..
  96. doi Lactoferrin and prevention of late-onset sepsis in the pre-term neonates
    P Manzoni
    Neonatal Intensive Care Units of S Anna Hospital, Turin, Italy
    Early Hum Dev 86:59-61. 2010
    ..Human (hLF) and bovine lactoferrin (bLF) share a high (77%) amino-acid homology, and the same N-terminal peptide responsible ..
  97. doi Simultaneous determination of vitexin-4''-O-glucoside, vitexin-2''-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC-ESI-MS/MS
    Wenjun Zhang
    College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China
    J Chromatogr B Analyt Technol Biomed Life Sci 878:1837-44. 2010
    ..VRH), rutin (RUT) and vitexin (VIT) in rat plasma after intravenous administration of hawthorn leaves flavonoids (HLF)...
  98. doi Non-ionic surfactant vesicles in pulmonary glucocorticoid delivery: characterization and interaction with human lung fibroblasts
    Carlotta Marianecci
    Department of Drug Chemistry and Technologies, Faculty of Pharmacy, University Sapienza, Rome, Italy
    J Control Release 147:127-35. 2010
    ..Primary human lung fibroblast (HLF) cells were used for in vitro investigation of vesicle tolerability, carrier-cell interaction, intracellular drug ..
  99. pmc The loss of circadian PAR bZip transcription factors results in epilepsy
    Frederic Gachon
    Department of Molecular Biology, National Center of Competence Research Frontiers in Genetics, Sciences III, University of Geneva, CH 1211 Geneva 4, Switzerland
    Genes Dev 18:1397-412. 2004
    DBP (albumin D-site-binding protein), HLF (hepatic leukemia factor), and TEF (thyrotroph embryonic factor) are the three members of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family...
  100. doi Interleukin-6 receptor superantagonist Sant7 inhibits TGF-beta-induced proliferation of human lung fibroblasts
    L Gallelli
    Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, Italy
    Cell Prolif 41:393-407. 2008
    ..the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either IL-6 or TGF-beta1, the effects on phosphorylation of mitogen-activated protein kinases (MAPK) ..
  101. pmc Expression of myelin basic protein (MBP) epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonal antibodies
    R Chignola
    Department of Pathology and Neurology Section, University of Verona, Italy
    Clin Exp Immunol 122:429-36. 2000
    ..30 MoAbs were selected on the basis of their different binding properties to: hLB-MBP, human lipid-free-MBP (hLF-MBP) and bovine lipid-free-MBP (bLF-MBP)...

Research Grants47

  1. Frank S Lee; Fiscal Year: 2014
    ..a family with hereditary erythrocytosis (increased red blood cell mass) due to a G537W missense mutation in the HIF2A gene, and another family with erythrocytosis due to a P317R missense mutation in the PHD2 gene...
  2. Yatrik M Shah; Fiscal Year: 2016
    ..Hypoxia-inducible factors (HIFs) are heterodimeric transcription factors consisting of an alpha subunit (HIF1a or HIF2a) and beta subunit (aryl hydrocarbon nuclear translocator (ARNT))...
  3. THERAPY FOR GASTROINTESTINAL DISORDERS
    Elizabeth Dial; Fiscal Year: 1999
    The main objective of this research proposal is to determine the efficacy of recombinant human lactoferrin (hLF) to treat gastrointestinal disorders induced by Helicobacter pylori infection or NSAID toxicity...
  4. Robert G Roeder; Fiscal Year: 2016
    ..domain of E2A to the DNA- binding domain of pre-B-cell leukemia factor 1 (PBX1) or hepatic leukemia factor (HLF), generating fusion proteins (E2A-PBX1 and E2A-HLF) that result in pediatric acute lymphoblastic leukemias (ALL) ..
  5. Christopher A Pennell; Fiscal Year: 2015
    ..Additionally, we will test the hypothesis that HIF2a is the molecular switch that induces expression of TLR2 ligands...
  6. Mechanism of Redox Regulation by Ape1/Ref-1
    Millie M Georgiadis; Fiscal Year: 2010
    ..function that serves to reduce a number of transcription factors including AP-1 (Fos/Jun), HIF-1a, NF-kB, PAX, HLF, p53, and others (reviewed in 1)...
  7. Hamid M Said; Fiscal Year: 2016
    ..studies, a yeast two-hybrid system was used to screen a human intestinal cDNA library and two proteins (PDZD11 and EPAS1) that interact with hSMVT were identified...
  8. Karen M Lyons; Fiscal Year: 2015
    ..cartilage and nucleus pulposus (NP), and that they mediate their effects in part through regulation of HIF1a and HIF2a, and in part by acting as escorts/chaperones for ECM proteins...
  9. Analysis of Cell Death Regulation in C. elegans
    Barbara Conradt; Fiscal Year: 2012
    ..We have also identified a three component pathway, the dnj- 11/MIDA1, ces-2/HLF, ces-1/Snail pathway, which is required for the asymmetric division of the NSM neuroblast and the exclusion of the ..
  10. E Jason Abel; Fiscal Year: 2016
    ..Our project is significant because RCC progression is driven by HIF signaling and increased understanding of the NF-?B/HIF2? axis may allow development of new therapies for this incurable disease. ..
  11. KLF2 Mediated HIF-1 Regulation and Macrophage Activation
    GANAPATI HOLANAGADDA MAHABALESHWAR; Fiscal Year: 2013
    ..Recent studies have identified Hypoxia-inducible Factor 1alpha(HlF-1 alpha) as a key regulator of macrophage activation function...
  12. Hypoxia-inducible factors in alcoholic liver disease
    Gyongyi Szabo; Fiscal Year: 2010
    ..These hypotheses will be tested in the following Specific Aims: AIM1 To evaluate and compare the role of HIF1a and HIF2a in the pathogenesis of alcoholic steatosis by investigating a) Whether an upregulation of HIF1alpha and/or ..
  13. REGULATORY MECHANISMS IN SURFACTANT SYNTHESIS
    CAROLE MENDELSON; Fiscal Year: 2002
    ..the levels of the O2-regulated TFs NF-kappaB, its inhibitory partner IkappaB, HIF-1alpha and the related protein EPAS1/HLF in lung tissues of fetal baboons during development and after organ culture in 2 percent vs...
  14. PREDOCTORAL FELLOWSHIPS FOR STUDENTS WITH DISABILITIES
    Shawn Terkhorn; Fiscal Year: 2006
    ..proteins, the oxygen sensor prolyl hydroxylase (PHD) and the transcription factor, hypoxia inducible factor (HlF). Work in progress suggests that PHDs sense oxygen by modification of specific proline residues in HIF...
  15. PARTICULATE CHROMIUM TOXICITY AND CARCINOGENESIS
    Steven Patierno; Fiscal Year: 2003
    ..and soluble hexavalent chromium compounds with normal human small airway epithelial cells (HSAE) and fibroblasts (HLF), which are representative target cells for chromium toxicity and carcinogenesis...
  16. Radioprotective Role of Slug in Bone Marrow Cells
    Wen Shu Wu; Fiscal Year: 2005
    ..Slug was identified as a functional target of the E2A-HLF fusion oncoprotein in pro-B-cell acute leukemias, in which the E2A-HLF fusion gene was generated by the t(17:19)(..
  17. Hypoxic Sensing Transcription Factor EPAS1 in Mice
    Joseph Garcia; Fiscal Year: 2004
    ..Endothelial PAS domain protein 1 (EPAS1), the second hypoxic-responsive member of the PAS family, is expressed at high levels in vascular endothelial ..
  18. Genetic Modifiers of p53
    Susan Evans; Fiscal Year: 2005
    ..By genome-wide chromosomal mapping analyses, we identified loci containing modifiers of p53, mop1 and mop2, centromeric and telomeric to p53 on mouse chromosome 11 that causes embryonic lethality when combined with a p53 ..
  19. APOPTOSIS OF CARDIAC MUSCLE AS A TARGET FOR GENE THERAPY
    Michael Schneider; Fiscal Year: 2002
    ..or baculovirus inhibitor of Apoptosis Proteins (IAPs); and a dominant- negative form of hepatic leukemia factor (HLF), a mammalian homologue for the transcription factor CES-2, a conserved cell death specification protein in ..
  20. Survival Signaling After Genotoxic Insult
    Susan Ceryak; Fiscal Year: 2009
    ..pharmacological and genetic approaches, by using relevant model systems of human diploid lung fibroblasts (HLF), and human large airway epithelial cells (HLAE) and studying the involvement of key signaling components of the ..
  21. Hypoxia-inducible factor1a in multistage carcinogenesis
    Jeffrey Arbeit; Fiscal Year: 2006
    ..3.1. Create Kl4-E6HIF-1alpha or K14-E6:HlF-1alpha delta ODD double transgenic mice and determine alterations in the biology of skin and cervical ..
  22. BRAIN GENOMIC RESPONSE TO HYPOXIA
    Frank Sharp; Fiscal Year: 2002
    ..Aim #3. Examine the genomic response to chronic hypoxia in HlF-1a knockout mice compared to wild type mice...
  23. Poor competition in hematopoietic stem/progenitor pools favors tumorigenesis
    James DeGregori; Fiscal Year: 2009
    ..To test our hypothesis, we will introduce the oncogenic Bcr-Abl and E2a-Hlf translocation products into mouse BM stem cells using retroviral transduction, and then reconstitute hematopoiesis ..
  24. Hypoxia-Inducible Factor 1 and Oxygen Homeostasis
    Gregg Semenza; Fiscal Year: 2009
    ..2. To investigate the role of HlF-1 in vascular progenitor cells...
  25. Synergistic activation of PTGS2 and CXCL8 by Ni and microbial stimuli
    Kelly Brant; Fiscal Year: 2008
    ..expression of PTGS2 and the immune-modulating chemokine CXCL8(IL-8) in cultured human lung fibroblast cells (HLF)...
  26. Mammalian Lactoferrin Receptors: Structure and Function
    Bo Lonnerdal; Fiscal Year: 2007
    ..Overall, our understanding of the physiological significance of Lf and its receptor will be increased. ..
  27. Signaling Pathways in Renal Fibrogenesis
    H Schnaper; Fiscal Year: 2008
    ..By elucidating the signaling events underlying kidney fibrosis we hope to better understand fibrogenic mechanisms and how to interrupt them. ..
  28. Particulate Matter/Mycoplasma Stress Interactions
    James Fabisiak; Fiscal Year: 2008
    ..organism capable of latent/subclinical infection or colonization to deliberately infect human lung fibroblasts (HLF) in vitro in order to study the molecular and cellular basis for the synergistic interactions with residual oil fly ..
  29. A Novel Regulator of Oxygen Homeostasis
    Frank Lee; Fiscal Year: 2009
    ..We anticipate that these studies will define a new pathway by which cells respond to hypoxia. ..
  30. Hypoxia inducible transcription in antitumor strategies
    Andrew Kung; Fiscal Year: 2005
    ..2. Search for small molecules that specifically perturb hypoxia- responsive transcription. 3. Attempt to create a mechanism-based imaging method for studying the effect of hypoxia-response inhibitors in animal tumors. ..
  31. ESTROGEN AND ENDOTHELIAL MIGRATION AND DIFFERENTIATION
    H Schnaper; Fiscal Year: 2002
    ..These studies will elucidate regulatory effects of estradiol on endothelial cell function. ..
  32. PATHOPHYSIOLOGY OF RENAL TUBULAR CELLS
    Frank Lee; Fiscal Year: 2002
    ....
  33. MECHANISMS OF ENDOTHELIAL GAPDH REGULATION BY HYPOXIA
    KRISTA GRAVEN; Fiscal Year: 2002
    ..abstract_text> ..
  34. PHARMACOGENOMIC STUDY OF ANTICONVULSANT THERAPY
    THOMAS FERRARO; Fiscal Year: 2003
    ....
  35. OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLS
    Mark Kelley; Fiscal Year: 2003
    ..abstract_text> ..
  36. Functional Studies of VHL by Conditional Gene Targeting
    Volker Haase; Fiscal Year: 2003
    ..Volker H. Haase, who is an Assistant Professor of Medicine in the University of Pennsylvania's Renal Division. Dr. Michael Madaio, Professor of Medicine, will function as Dr. Haase's mentor for this grant. ..
  37. VHL FUNCTION BY CONDITIONAL GENE TARGETING
    Volker Haase; Fiscal Year: 2003
    ..He is absolutely committed to a career centered on basic research in an academic Nephrology division applying the techniques learned during the award period. ..
  38. Renal Cell Injury in Diabetes
    Karen Block; Fiscal Year: 2008
    ..Elucidating these events may allow future modulation of the enzymatic complex(es) to prevent oxidative stress implicated in cellular injury in diabetes. [unreadable] [unreadable]..
  39. TGF-beta and Cytoskeletal Signaling in Mesangial Cell
    H Schnaper; Fiscal Year: 2005
    ..These studies will determine whether increased cytoskeletal tension is required for RGTPase effects on collagen expression. ..
  40. Kidneys Fail:Translating basic mechanisms into therapies
    H Schnaper; Fiscal Year: 2005
    ..Our past experience is that these more social events are an important part of encouraging collaboration within and between the renal and non-renal communities. ..
  41. Molecular Mechanisms of the Hypoxic Response
    Frank Lee; Fiscal Year: 2006
    ..We anticipate that the proposed studies will enlarge our understanding of both the molecular response to hypoxia and the role of a novel postranslational modification--proline hydroxylation. ..
  42. MOLECULAR MECHANISMS OF RENAL INJURY
    Volker Haase; Fiscal Year: 2007
    ..Data generated from this grant would be used to guide a grant proposal for 5-year NIH funding. [unreadable] [unreadable] [unreadable]..
  43. Therapeutic/Mechanistic Role of Ape1 in Germ Cell Tumors
    Mark Kelley; Fiscal Year: 2007
    ..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
  44. Hypoxic signaling in hepatocytes
    Volker Haase; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  45. Imbalancing DNA BER to enhance Ovarian Tumor Sensitivity
    Mark Kelley; Fiscal Year: 2008
    ..abstract_text> ..