Genomes and Genes
Gene Symbol: EPAS1
Description: endothelial PAS domain protein 1
Alias: ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-2-alpha, HIF2-alpha, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, hypoxia-inducible factor 2 alpha, member of PAS protein 2
Publications209 found, 100 shown here
- E2A-HLF-mediated cell transformation requires both the trans-activation domains of E2A and the leucine zipper dimerization domain of HLFT Yoshihara
Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Mol Cell Biol 15:3247-55. 1995The E2A-HLF fusion gene, formed by the t(17;19)(q22;p13) translocation in childhood acute pro-B-cell leukemia, encodes a hybrid protein that contains the paired trans-activation domains of E2A (E12/E47) linked to the basic region/leucine ..
- Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau proteinM Ohh
Department of Medicine, Brigham and Womens Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Cell Biol 2:423-7. 2000..These results strengthen the link between overaccumulation of HIF and development of VHL disease...
- Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancerM R Kelley
Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 702 Barnhill Drive, Indianapolis, Indiana 46202, USA
Clin Cancer Res 7:824-30. 2001..box containing genes (PAX), nuclear factor-kappaB, hypoxia-inducible factor alpha (HIF-1alpha), HIF-like factor (HLF), p53, and others. The expression of Ape1/ref-1 in prostate has not been characterized previously...
- Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutationsLianjie Li
Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Harvard Medical School, 44 Binney Street, Mayer 457, Boston, MA 02115, USA
Mol Cell Biol 27:5381-92. 2007..Therefore, quantitative differences with respect to HIF deregulation are sufficient to account for the differential risks of kidney cancer linked to VHL mutations...
- Assessment of intestinal absorption of vitexin-2''-o-rhamnoside in hawthorn leaves flavonoids in rat using in situ and in vitro absorption modelsYing ai Xu
Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai, PR China
Drug Dev Ind Pharm 34:164-70. 2008..the absorption mechanism of vitexin-2''-O-rhamnoside, the index component in hawthorn leaves flavonoids (HLF) in the rat intestine, using two different absorption models, the in situ single-pass intestinal perfusion and the ..
- Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensorJens Köditz
Department of Heart and Circulatory Physiology, Center of Physiology and Pathophysiology, Georg August University Gottingen, Gottingen, Germany
Blood 110:3610-7. 2007..These data demonstrate that PHD-dependent oxygen-sensing recruits both the hypoxia-inducible factor (HIF) and ATF-4 systems, and hence not only confers adaptive responses but also cell fate decisions...
- The peptidyl prolyl cis/trans isomerase FKBP38 determines hypoxia-inducible transcription factor prolyl-4-hydroxylase PHD2 protein stabilitySandra Barth
Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
Mol Cell Biol 27:3758-68. 2007..Downregulation of FKBP38 did not affect PHD2 mRNA levels but prolonged PHD2 protein stability, suggesting that FKBP38 is involved in PHD2 protein regulation...
- Prolyl-4-hydroxylase PHD2- and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesisM R Bordoli
Institute of Physiology and Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland
Oncogene 30:548-60. 2011..Our data suggest that PHD2/HIF-2/amphiregulin signaling has a critical role in the regulation of breast tumor progression and propose PHD2 as a potential tumor suppressor in breast cancer...
- Thymosin β4 expression correlates with lymph node metastasis through hypoxia inducible factor-α induction in breast cancerSun Young Yoon
Department of Life Science, Sookmyung Women s University, Yongsan gu, Seoul, Republic of Korea
Oncol Rep 25:23-31. 2011..Ultimately, these results highlight Tβ4 as a potentially therapeutic target in malignant cancers...
- Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cellsH Tian
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA
Genes Dev 11:72-82. 1997Here we describe the cloning and characterization of a PAS domain transcription factor termed endothelial PAS-1 (EPAS1)...
- Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathwayJ B Hogenesch
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, Illinois 60611, USA
J Biol Chem 272:8581-93. 1997..studies, yeast two-hybrid analysis, and transient transfection experiments demonstrated that MOP1 and MOP2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo...
- A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1alpha regulates the VEGF expression and is potentially involved in lung and vascular developmentM Ema
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980 77, Japan
Proc Natl Acad Sci U S A 94:4273-8. 1997..The HIF1alpha-like factor (HLF) encoded by the isolated cDNA bound the hypoxia-response element (HRE) found in enhancers of genes for ..
- The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysisP H Maxwell
Wellcome Trust Centre for Human Genetics, Oxford, UK
Nature 399:271-5. 1999..Thus, constitutive HIF-1 activation may underlie the angiogenic phenotype of VHL-associated tumours. The pVHL/HIF-1 interaction provides a new focus for understanding cellular oxygen sensing...
- Hypoxia inducible factor-alpha binding and ubiquitylation by the von Hippel-Lindau tumor suppressor proteinM E Cockman
Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom
J Biol Chem 275:25733-41. 2000....
- HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensingM Ivan
Dana Farber Cancer Institute and Brigham and Women s Hospital, Howard Hughes Medical Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA
Science 292:464-8. 2001..Because proline hydroxylation requires molecular oxygen and Fe(2+), this protein modification may play a key role in mammalian oxygen sensing...
- Contrasting effects on HIF-1alpha regulation by disease-causing pVHL mutations correlate with patterns of tumourigenesis in von Hippel-Lindau diseaseS C Clifford
Section of Medical and Molecular Genetics, Department of Paediatrics and Child Health, University of Birmingham, The Medical School, Edgbaston, Birmingham B15 2TT, UK
Hum Mol Genet 10:1029-38. 2001..Our findings are consistent with impaired ability to degrade HIF-alpha subunit being required for HAB development and RCC susceptibility...
- Asparagine hydroxylation of the HIF transactivation domain a hypoxic switchDavid Lando
Department of Molecular Biosciences Biochemistry, Adelaide University, SA 5005, Australia
Science 295:858-61. 2002..Full induction of HIF-1alpha and -2alpha, therefore, relies on the abrogation of both Pro and Asn hydroxylation, which during normoxia occur at the degradation and COOH-terminal transactivation domains, respectively...
- Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)Gerd Elvert
Zentrum für Molekulare Medizin der Universität zu Köln, Joseph Stelzmann Strasse 9, 50931 Koln, Germany
J Biol Chem 278:7520-30. 2003....
- Tat-binding protein-1, a component of the 26S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau proteinPaul G Corn
Department of Medicine, Howard Hughes Medical Institute and Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Nat Genet 35:229-37. 2003..Thus, the ability of pVHL to degrade Hif1 alpha depends in part on its interaction with TBP-1 and suggests a new mechanism for Hif1 alpha stabilization in some pVHL-deficient tumors...
- Differential roles of hypoxia-inducible factor 1alpha (HIF-1alpha) and HIF-2alpha in hypoxic gene regulationCheng Jun Hu
Abramson Family Cancer Research Institute Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA
Mol Cell Biol 23:9361-74. 2003..Importantly, HIF-1alpha (and not HIF-2alpha) stimulates glycolytic gene expression in both types of cells, clearly showing for the first time that HIF-1alpha and HIF-2alpha have unique targets...
- Inhibition of HIF2alpha is sufficient to suppress pVHL-defective tumor growthKeiichi Kondo
Department of Adult Oncology, Dana Farber Cancer Institute and Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts, USA
PLoS Biol 1:E83. 2003..These results establish that tumor suppression by pVHL is linked to regulation of HIF target genes...
- Prolonged hypoxia differentially regulates hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha expression in lung epithelial cells: implication of natural antisense HIF-1alphaTokujiro Uchida
Department of Physiology, INSERM U426, Faculte de Medecine Xavier Bichat, Universite Paris 7, 75870 Paris, Cedex 18, France
J Biol Chem 279:14871-8. 2004..This trans-regulation between HIF-1alpha and HIF-2alpha during hypoxia likely conveys target gene specificity...
- Inhibition of hypoxia-inducible factor is sufficient for growth suppression of VHL-/- tumorsMichael Zimmer
Hematology Oncology Unit, Department of Medicine, Massachusetts General Hospital and the Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA
Mol Cancer Res 2:89-95. 2004..These data suggest that deregulation of hypoxia-inducible genes in VHL-/- cells can be attributed mainly to deregulation of HIF and validate HIF as a therapeutic anticancer drug target...
- Differentiating the functional role of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha (EPAS-1) by the use of RNA interference: erythropoietin is a HIF-2alpha target gene in Hep3B and Kelly cellsChristina Warnecke
Department of Nephrology and Medical Intensive Care, Virchow Clinic, Charite University Medicine, Berlin, Germany
FASEB J 18:1462-4. 2004..In conclusion, we found a remarkably restricted target gene specificity of the HIFalpha subunits, which can be overcome in cells with perturbations in the pVHL/HIF system and under forced expression...
- Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factorRebecca J Appelhoff
Henry Wellcome Bldg of Genomic Medicine, Roosevelt Drive, Headington, Oxford, UK
J Biol Chem 279:38458-65. 2004....
- Activity of hypoxia-inducible factor 2alpha is regulated by association with the NF-kappaB essential modulatorCameron P Bracken
School of Molecular and Biomedical Science and the Centre for the Molecular Genetics of Development, University of Adelaide, Adelaide, South Australia, 5005, Australia
J Biol Chem 280:14240-51. 2005....
- Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinomaRaju R Raval
The Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford OX3 7BN, United Kingdom
Mol Cell Biol 25:5675-86. 2005..These findings indicate that therapeutic approaches to targeting of the HIF system, at least in this setting, will need to take account of HIF isoform-specific functions...
- The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletesJennifer Henderson
Department of Molecular and Clinical Genetics, Royal Prince Alfred Hospital and Central Clinical School, The University of Sydney K25, Camperdown, 2050, Australia
Hum Genet 118:416-23. 2005b>EPAS1 is a gene involved in complex oxygen sensing. It is expressed in microvascular endothelial cells, lung epithelial cells, cardiac myocytes and the brain...
- A family with erythrocytosis establishes a role for prolyl hydroxylase domain protein 2 in oxygen homeostasisMelanie J Percy
Department of Haematology, Belfast City Hospital, Belfast BT9 7AB, Northern Ireland, United Kingdom
Proc Natl Acad Sci U S A 103:654-9. 2006..Our findings indicate that PHD2 is critical for normal regulation of HIF in humans...
- Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environmentCameron P Bracken
School of Molecular and Biomedical Science, University of Adelaide, South Australia
J Biol Chem 281:22575-85. 2006..Interestingly, the initial stabilization of HIF-alpha prior to transactivation up-regulation predicted from in vitro derived hydroxylation data is only true for a subset of cells...
- Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alphaToshiaki Shinojima
Department of Urology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
Carcinogenesis 28:529-36. 2007..HIF-1alpha may therefore represent an important target molecule for RCC therapy; however, HIF-2alpha should be targeted in HIF-1alpha defective renal cancer cells...
- Recruitment of HIF-1alpha and HIF-2alpha to common target genes is differentially regulated in neuroblastoma: HIF-2alpha promotes an aggressive phenotypeLinda Holmquist-Mengelbier
Division of Molecular Medicine, Department of Laboratory Medicine, Lund University, University Hospital MAS, SE 205 02 Malmo, Sweden
Cancer Cell 10:413-23. 2006..Our results demonstrate the relevance of HIF-2alpha in neuroblastoma progression and have general tumor biological implications...
- Iron-regulatory proteins limit hypoxia-inducible factor-2alpha expression in iron deficiencyMayka Sanchez
Molecular Medicine Partnership Unit, Im Neuenheimer Feld 153, 69120 Heidelberg, Germany
Nat Struct Mol Biol 14:420-6. 2007..element (IRE) in the 5' untranslated region of the messenger RNA encoding endothelial PAS domain protein-1, EPAS1 (also called hypoxia-inducible factor-2alpha, HIF2alpha)...
- HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activityJohn D Gordan
Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA
Cancer Cell 11:335-47. 2007..Enhanced c-Myc activity likely contributes to HIF-2alpha-mediated neoplastic progression following loss of the VHL tumor suppressor and influences the behavior of hypoxic tumor cells...
- Stromal expression of hypoxia regulated proteins is an adverse prognostic factor in colorectal carcinomasArjen H G Cleven
Departments of Pathology, GROW Research Institute, University Hospital Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands
Cell Oncol 29:229-40. 2007..We studied the role of protein expression of hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, carbonic anhydrase 9 (CA9) and glucose transporter 1 (GLUT1) in patients with colorectal adenocarcinomas...
- Novel exon 12 mutations in the HIF2A gene associated with erythrocytosisMelanie J Percy
Department of Haematology, Belfast City Hospital, Belfast, United Kingdom
Blood 111:5400-2. 2008..This raises the possibility of HIF2A mutations being associated with other cases of erythrocytosis...
- The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factorsChristina Warnecke
Department of Nephrology and Hypertension, University Clinic Erlangen, Germany
Exp Cell Res 314:2016-27. 2008..Thus, HIF-2alpha effects are modulated by a number of intrinsic and extrinsic factors which may be most relevant in tumour cells...
- Autosomal dominant erythrocytosis and pulmonary arterial hypertension associated with an activating HIF2 alpha mutationDaniel P Gale
Blood 112:919-21. 2008
- Familial erythrocytosis arising from a gain-of-function mutation in the HIF2A gene of the oxygen sensing pathwayMelanie J Percy
Department of Haematology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, UK
Ulster Med J 77:86-8. 2008..Continued study of idiopathic cases of erythrocytosis with raised serum Epo will reveal whether HIF-2 alpha will be a major cause of erythrocytosis on par with VHL...
- Differential dependence of hypoxia-inducible factors 1 alpha and 2 alpha on mTORC1 and mTORC2Alfredo Toschi
Department of Biological Sciences, Hunter College, The City University of New York, New York, New York 10021, USA
J Biol Chem 283:34495-9. 2008..Constitutive expression of HIF1 alpha and HIF2 alpha has been linked to a number of human cancers, especially renal cell carcinoma (RCC), in which HIF2 alpha ..
- HIF-1alpha and HIF-2alpha have divergent roles in colon cancerTakaaki Imamura
Gastrointestinal Unit, Massachusetts General Hospital, Boston, MA 02114, USA
Int J Cancer 124:763-71. 2009..In particular, HIF-1alpha promoted the growth of SW480 colon cancer cells but HIF-2alpha appeared to restrain growth. Consequently, therapeutic approaches that target HIF may need to consider these isoform-specific properties...
- Hypoxia-inducible factor-1alpha and -2alpha are expressed in most rectal cancers but only hypoxia-inducible factor-1alpha is associated with prognosisS Rasheed
Department of Surgery, St Mark s Hospital, Harrow, Middlesex, UK
Br J Cancer 100:1666-73. 2009..Our results indicate that HIF-1alpha, but not HIF-2alpha, might be used as a marker of prognosis, in addition to methods currently used, to enhance patient management...
- Regulation of hypoxia-inducible factor 2alpha signaling by the stress-responsive deacetylase sirtuin 1Elhadji M Dioum
Veterans Affairs North Texas Health Care System, Department of Medicine, 4500 South Lancaster Road, Dallas, TX 75216, USA
Science 324:1289-93. 2009..Thus, Sirt1 promotes HIF-2 signaling during hypoxia and likely other environmental stresses...
- HIF2alpha inhibition promotes p53 pathway activity, tumor cell death, and radiation responsesJessica A Bertout
Abramson Family Cancer Research Institute, Abramson Cancer Center, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 106:14391-6. 2009..These results correlate with altered p53 phosphorylation and target gene expression in untreated human tumor samples and show that HIF2alpha likely contributes to tumor cell survival including during radiation therapy...
- Human cancers converge at the HIF-2alpha oncogenic axisAleksandra Franovic
Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
Proc Natl Acad Sci U S A 106:21306-11. 2009....
- Prolyl hydroxylases 2 and 3 act in gliomas as protective negative feedback regulators of hypoxia-inducible factorsAnne Theres Henze
Institute of Neuropathology, Giessen, Germany
Cancer Res 70:357-66. 2010....
- The NADPH oxidase subunit p22phox inhibits the function of the tumor suppressor protein tuberinKaren Block
Department of Medicine, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
Am J Pathol 176:2447-55. 2010..Our data provide the first evidence that p22(phox)-based Nox oxidases maintain HIF-2alpha protein expression through inactivation of tuberin and downstream activation of ribosomal protein S6 kinase 1/4E-BP1 pathway...
- Natural selection on EPAS1 (HIF2alpha) associated with low hemoglobin concentration in Tibetan highlandersCynthia M Beall
Department of Anthropology, Case Western Reserve University, Cleveland, OH 44106 7125, USA
Proc Natl Acad Sci U S A 107:11459-64. 2010..m) with closely related lowland Han revealed a genome-wide significant divergence across eight SNPs located near EPAS1. This gene encodes the transcription factor HIF2alpha, which stimulates production of red blood cells and thus ..
- Sequencing of 50 human exomes reveals adaptation to high altitudeXin Yi
BGI Shenzhen, Shenzhen 518083, China
Science 329:75-8. 2010..The strongest signal of natural selection came from endothelial Per-Arnt-Sim (PAS) domain protein 1 (EPAS1), a transcription factor involved in response to hypoxia...
- A genome-wide search for signals of high-altitude adaptation in TibetansShuhua Xu
Chinese Academy of Sciences and Max Planck Society Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Mol Biol Evol 28:1003-11. 2011..2 and 2p21. EGLN1 (or HIFPH2, MIM 606425) and EPAS1 (or HIF2A, MIM 603349), both related to hypoxia-inducible factor, were found most differentiated in the two ..
- Hypoxia-inducible factors and the response to hypoxic stressAmar J Majmundar
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Mol Cell 40:294-309. 2010..These transcription factors function in normal tissue homeostasis and impinge on critical aspects of disease progression and recovery. Insights from basic HIF biology are being translated into pharmaceuticals targeting the HIF pathway...
- Genetic variations in Tibetan populations and high-altitude adaptation at the HimalayasYi Peng
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Kunming, China
Mol Biol Evol 28:1075-81. 2011..combination with the reported data, we identified strong signals of selective sweep in two hypoxia-related genes, EPAS1 and EGLN1...
- Cardiopulmonary function in two human disorders of the hypoxia-inducible factor (HIF) pathway: von Hippel-Lindau disease and HIF-2alpha gain-of-function mutationFederico Formenti
Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Rd, Oxford, OX1 3PT, UK
FASEB J 25:2001-11. 2011....
- Opposite effects of HIF-1α and HIF-2α on the regulation of IL-8 expression in endothelial cellsUrszula Florczyk
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
Free Radic Biol Med 51:1882-92. 2011..However, despite stabilization of both isoforms in hypoxia the effect of HIF-1 is predominant, and downregulation of IL-8 expression in hypoxia is caused by attenuation of Nrf2 and c-Myc...
- HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cellsShiro Koizume
Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
Nucleic Acids Res 40:5389-401. 2012..These results suggest the presence of a stress-responsive transcription mediated by the HIF2α/Sp1/HDAC4 network and explain how CCC shed their procoagulant activity under hypoxia...
- The acetylase/deacetylase couple CREB-binding protein/Sirtuin 1 controls hypoxia-inducible factor 2 signalingRui Chen
Department of Medicine, Veterans Affairs North Texas Health Care System, Dallas, TX 75216, USA
J Biol Chem 287:30800-11. 2012..Maximal CBP/HIF-2 signaling requires intact CBP acetyltransferase activity in both Hep3B cells as well as in mice...
- Somatic HIF2A gain-of-function mutations in paraganglioma with polycythemiaZhengping Zhuang
Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
N Engl J Med 367:922-30. 2012..Here we report two novel somatic gain-of-function mutations in the gene encoding hypoxia-inducible factor 2α (HIF2A) in two patients, one presenting with paraganglioma and the other with paraganglioma and somatostatinoma, both of ..
- Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomasChunzhang Yang
Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20894, USA
Blood 121:2563-6. 2013..Here, we report 2 additional unique HIF2A mutations, which disrupt the hydroxylation domain recognized by prolyl hydroxylase domain-2 containing protein, ..
- Regulation of HIF prolyl hydroxylases by hypoxia-inducible factorsOlga Aprelikova
Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
J Cell Biochem 92:491-501. 2004..These data further advance our understanding of the differential role of HIF factors and putative feedback loop in HIF regulation...
- Hypoxia-inducible factor-2alpha correlates to distant recurrence and poor outcome in invasive breast cancerKarolina Helczynska
Center for Molecular Pathology, Department of Laboratory Medicine, University Hospital MAS, Malmo, Sweden
Cancer Res 68:9212-20. 2008..At prolonged hypoxia, HIF-2alpha remained accumulated, whereas HIF-1alpha protein levels decreased, in agreement with the oxygen level and time-dependent induction of HIFs recently reported in neuroblastoma...
- Determination and comparison of specific activity of the HIF-prolyl hydroxylasesJason R Tuckerman
Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN, UK
FEBS Lett 576:145-50. 2004....
- The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylasesPeppi Koivunen
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, University of Oulu, FIN 90014, Oulu, Finland
J Biol Chem 281:28712-20. 2006..Even this value is high compared with tissue O(2) levels, indicating that HIF-P4Hs are effective oxygen sensors...
- Tumor cell invasion of von Hippel Lindau renal cell carcinoma cells is mediated by membrane type-1 matrix metalloproteinaseBrenda L Petrella
Department of Medicine, Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, NH, USA
Mol Cancer 5:66. 2006..The aim of this study was to investigate the contribution of MT1-MMP to the invasive potential of RCC cells using in vitro type I collagen degradation and invasion assays...
- A novel heterozygous HIF2AM535I mutation reinforces the role of oxygen sensing pathway disturbances in the pathogenesis of familial erythrocytosisMaurizio Martini
Department of Pathology, Catholic University, Rome, Italy
Haematologica 93:1068-71. 2008b>HIF2A transcription factor plays a central role in the regulation of the hypoxia responding pathway in mammalian cells, by modulating erythropoiesis and angiogenesis...
- Heterogeneity in binding and gene-expression regulation by HIF-2alphaShiro Koizume
Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
Biochem Biophys Res Commun 371:251-5. 2008..These results demonstrate that, unlike HIF-1alpha, HIF-2alpha differentially binds and regulates transcription under hypoxia...
- Clock genes in the heart: characterization and attenuation with hypertrophyM E Young
Department of Internal Medicine, Division of Cardiology, University of Texas-Houston Medical School, Houston, TX 77030, USA
Circ Res 88:1142-50. 2001..heart all mammalian homologues of known Drosophila clock genes (bmal1, clock, cry1, cry2, per1, per2, per3, dbp, hlf, and tef) show circadian patterns of expression and that the induction of clock output genes (the PAR [rich in ..
- One hundred sixteen cases of acute liver failure treated with MARSG Novelli
Policlinico Umberto I, University of Rome La Sapienza, Rome, Italy
Transplant Proc 37:2557-9. 2005..The mean length of application was about 9 hours (range: 8-20). MARS treatment was carried out in HLF patients with continuous acute-on-chronic hepatic failure dialisate flow similar to continuous veno venus ..
- PDP1, a novel Drosophila PAR domain bZIP transcription factor expressed in developing mesoderm, endoderm and ectoderm, is a transcriptional regulator of somatic muscle genesS C Lin
Department of Biochemistry and Molecular Biology M C536, University of Illinois College of Medicine, Chicago 60612, USA
Development 124:4685-96. 1997..a novel transcription factor that is highly homologous to the PAR subfamily of mammalian bZIP transcription factors HLF, DBP and VBP/TEF...
- Thoracic myelopathy caused by ossification of the ligamentum flavum. Clinicopathologic study and surgical treatmentK Okada
Department of Orthopaedic Surgery, Kagawa Medical School, Japan
Spine (Phila Pa 1976) 16:280-7. 1991..cells were found in the increased and swollen collagen fibers forming the hypertrophic ligamentum flavum (HLF). Ossification extended along the superficial layer of HLF...
- Inhibitory effects of lactoferrin on growth and biofilm formation of Porphyromonas gingivalis and Prevotella intermediaHiroyuki Wakabayashi
Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, 5 1 83 Higashihara, Zama, Kanagawa 228 8583, Japan
Antimicrob Agents Chemother 53:3308-16. 2009..gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >or=130 microg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >or=6 microg/ml of bLF-..
- Human lactoferrin activates NF-kappaB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide-stimulated TLR4 signalingKen Ando
Tokyo University of Pharmacy and Life Sciences, Japan
FEBS J 277:2051-66. 2010..Here we reveal the signal transduction pathway responsible for human LF (hLF)-triggered nuclear factor-kappaB (NF-kappaB) activation...
- Variation in antimicrobial activity of lactoferricin-derived peptides explained by structure modellingSebastien Farnaud
Randall Division of Cell and Molecular Biophysics, King s College London, New Hunt s House, Guy s Campus, St Thomas Street, London SE1 1UL, UK
FEMS Microbiol Lett 238:221-6. 2004..differences observed between the bovine lactoferrin (BLf) and LfcinB were also found between the human lactoferrin (HLf) and the LfcinH...
- Antimicrobial mechanism of action of transferrins: selective inhibition of H+-ATPaseMaría T Andrés
Laboratory of Oral Microbiology, School of Stomatology, Oviedo, Spain
Antimicrob Agents Chemother 54:4335-42. 2010..In anaerobiosis, P. aeruginosa cells were not susceptible to lactoferrin (hLf) or transferrin (hTf)...
- Intravenous infusion of erythromycin inhibits CXC chemokine production, but augments neutrophil degranulation in whole blood stimulated with Streptococcus pneumoniaeM J Schultz
Laboratory of Experimental Internal Medicine, Department of Intensive Care, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
J Antimicrob Chemother 46:235-40. 2000..products bactericidal/permeability increasing protein (BPI), human neutrophil elastase (HNE) and human lactoferrin (HLF) upon stimulation with S. pneumoniae...
- Hispanic Labor Friends Initiative: supporting vulnerable womenCambria Jones Hazard
College of Nursing, Brigham Young University, Provo, UT, USA
MCN Am J Matern Child Nurs 34:115-21. 2009..To evaluate the qualitative aspects of the Hispanic Labor Friends Initiative...
- Effects of Echinostoma caproni infection on the neutral and polar lipids of intestinal and non-intestinal organs in the BALB/c mouse as determined by high-performance thin-layer chromatographyJessica Counihan
Department of Chemistry, Lafayette College, Easton, PA 18042, USA
Parasitol Res 107:947-53. 2010..Lipids were determined by high-performance thin-layer chromatography (HPTLC) with Analtech 10 x 20 cm HPTLC-HLF silica gel plates...
- Comprehensive characterization of the site-specific N-glycosylation of wild-type and recombinant human lactoferrin expressed in the milk of transgenic cloned cattleTian Yu
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People s Republic of China
Glycobiology 21:206-24. 2011..Here we determined the site-specific N-glycosylation profile of human lactoferrin (hLF) and recombinant human lactoferrin (rhLF) expressed in the milk of transgenic cloned cattle...
- Recombinant human lactoferrin treatment for global health issues: iron deficiency and acute diarrheaDelia R Bethell
Ventria Bioscience, 4110 N Freeway Boulevard, Sacramento, California 95834, USA
Biometals 17:337-42. 2004..of the diarrheal associated organisms: rotavirus, ETEC, cholera, salmonella, and shigella by human lactoferrin (hLF) and human lysozyme...
- Inhibition of poly adenosine diphosphate-ribose polymerase decreases hepatocellular carcinoma growth by modulation of tumor-related gene expressionRosa Quiles-Perez
San Cecilio University Hospital, Granada, Spain
Hepatology 51:255-66. 2010..growth factor receptor-1, VEGFR1], epidermal growth factor receptor [EPAS1]/hypoxia-inducible factor 2 [HIF2A], EGLN1 [PHD2], epidermal growth factor receptor [EGFR], MYC, JUND, SPP1 [OPN], hepatocyte growth factor [HGF]) ..
- HIF-2alpha regulates murine hematopoietic development in an erythropoietin-dependent mannerMarzia Scortegagna
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8573, USA
Blood 105:3133-40. 2005..We previously reported that mice lacking wild-type HIF-2alpha, encoded by the EPAS1 gene, exhibit pancytopenia. In this study, we have characterized the etiology of this hematopoietic phenotype...
- [Mammary gland dual-expression construct containing prolactin and foreign DNAs can promote the expression of foreign proteins in mammary cells]Feng Tao Liu
Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai 200 040, China
Yi Chuan 26:567-73. 2004To investigate the feasibility in the promotion of the expression of foreign proteins, such as human lactinferrin (hLF) and thrombopoietin (TPO), in the transgenic animals-mammary gland bioreactor by bovine prolactin (bPRL), two types of ..
- Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytesS Ikushima
Department of Experimental Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Proc Natl Acad Sci U S A 94:2609-14. 1997The E2A-HLF (hepatic leukemia factor) oncoprotein, generated in pro-B lymphocytes by fusion of the trans-activation domain of E2A to the basic region/leucine zipper (bZIP) domain of HLF, functions as an anti-apoptotic transcription ..
- Environmental impacts on offspring survival during the lambing period in central PatagoniaF Coronato
Centro Nacional Patagónico CONICET, Puerto Madryn, Argentina
Int J Biometeorol 43:113-8. 1999..2-week averages of temperature (Tf), wind speed (Vf) and total precipitation (Pf), the average heat loss in sheep (HLf) was 40.40 - 2.07Tf + 5.64Vf + 0.04Pf. (r2 = 0.95)...
- 99mTc-Labeled UBI 29-41 peptide for monitoring the efficacy of antibacterial agents in mice infected with Staphylococcus aureusPeter H Nibbering
Department of Infectious Diseases, Leiden University Medical Center C5 P, PO Box 9600, 2300 RC Leiden, The Netherlands
J Nucl Med 45:321-6. 2004....
- Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAsNikolay G Kolev
Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06536, USA
Genes Dev 19:2583-92. 2005..cell extracts requires the U7 small nuclear ribonucleoprotein (U7 snRNP) and an unidentified heat-labile factor (HLF)...
- The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in miceMichele M Hickey
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
J Clin Invest 120:827-39. 2010..HIF-2alpha activity was increased in lungs from VhlR/R mice, and heterozygosity for Hif2a, but not Hif1a, genetically suppressed both the polycythemia and pulmonary hypertension in the VhlR/R mice...
- Effect of carrier fluid viscosity on retention time and resolution in gravitational field-flow fractionationSeungho Lee
Department of Chemistry, Hannam University, Daejeon, South Korea
Anal Chem 83:3343-51. 2011..focused layers in the channel thickness where force due to gravity is exactly opposed by hydrodynamic lift forces (HLF)...
- Different elution modes and field programming in gravitational field-flow fractionation: field programming using density and viscosity gradientsJana Plocková
Institute of Analytical Chemistry, AS CR, 611 42 Brno, Czech Republic
J Chromatogr A 1118:253-60. 2006..e. factors influencing hydrodynamic lift forces (HLF)...
- Mycoplasma fermentans and TNF-beta interact to amplify immune-modulating cytokines in human lung fibroblastsJames P Fabisiak
Department of Environmental and Occupational Health, Center for Free Radical and Antioxidant Health, University of Pittsburgh Graduate School of Public Health, College of Medicine, Pittsburgh, PA 15219 3130, USA
Am J Physiol Lung Cell Mol Physiol 291:L781-93. 2006..Human lung fibroblasts (HLF) were exposed to live M...
- Fine mapping of a major QTL influencing morphine preference in C57BL/6 and DBA/2 mice using congenic strainsGlenn A Doyle
Department of Psychiatry, Center for Neurobiology and Behavior, University of Pennsylvania, Philadelphia, PA 19104, USA
Neuropsychopharmacology 33:2801-9. 2008..Here, we report the refinement of the Chr. 10 QTL in subcongenic strains of D2.B6-Mop2 congenic mice described previously...
- DNA-binding specificity of the PAR basic leucine zipper protein VBP partially overlaps those of the C/EBP and CREB/ATF families and is influenced by domains that flank the core basic regionN B Haas
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
Mol Cell Biol 15:1923-32. 1995The PAR subfamily of basic leucine zipper (bZIP) factors comprises three proteins (VBP/TEF, DBP, and HLF) that have conserved basic regions flanked by proline- and acidic-amino-acid-rich (PAR) domains and functionally compatible leucine ..
- DNA-binding and transcriptional regulatory properties of hepatic leukemia factor (HLF) and the t(17;19) acute lymphoblastic leukemia chimera E2A-HLFS P Hunger
Department of Pathology, Stanford University School of Medicine, California 94305
Mol Cell Biol 14:5986-96. 1994The t(17;19) translocation in acute lymphoblastic leukemias results in creation of E2A-hepatic leukemia factor (HLF) chimeric proteins that contain the DNA-binding and protein dimerization domains of the basic leucine zipper (bZIP) ..
- Effect of ovotransferrin and lactoferrins on Chlamydophila psittaci adhesion and invasion in HD11 chicken macrophagesDelphine Sylvie Anne Beeckman
Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium
Vet Res 38:729-39. 2007The effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular pathogen Chlamydophila (Cp...
- Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactionsDonatella Aldinucci
Experimental Oncology 2, Centro di Riferimento Oncologico, I R C C S, Aviano PN, Italy
Int J Cancer 122:769-76. 2008..CD40 engagement and also by coculturing L-1236 cells on primary stromal fibroblasts from HL-involved lymph nodes (HLF). Coculture experiments indicated that a direct contact of H-RS cells induces HLF cells to produce CCL5...
- The growth inhibition of hepatocellular and cholangiocellular carcinoma cells by gemcitabine and the roles of extracellular signal-regulated and checkpoint kinasesKazuya Matsumoto
Second Department of Internal Medicine, Tottori University School of Medicine, Tottori, Japan
Oncol Rep 20:863-72. 2008..After HCC cells (HepG2, Hep3B, HLF and PLC/PRF/5) and CCC cells (HuCCT-1) were treated with gemcitabine, cellular growth, cell cycle, nuclear ..
- Lactoferrin and prevention of late-onset sepsis in the pre-term neonatesP Manzoni
Neonatal Intensive Care Units of S Anna Hospital, Turin, Italy
Early Hum Dev 86:59-61. 2010..Human (hLF) and bovine lactoferrin (bLF) share a high (77%) amino-acid homology, and the same N-terminal peptide responsible ..
- Simultaneous determination of vitexin-4''-O-glucoside, vitexin-2''-O-rhamnoside, rutin and vitexin from hawthorn leaves flavonoids in rat plasma by UPLC-ESI-MS/MSWenjun Zhang
College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, PR China
J Chromatogr B Analyt Technol Biomed Life Sci 878:1837-44. 2010..VRH), rutin (RUT) and vitexin (VIT) in rat plasma after intravenous administration of hawthorn leaves flavonoids (HLF)...
- Non-ionic surfactant vesicles in pulmonary glucocorticoid delivery: characterization and interaction with human lung fibroblastsCarlotta Marianecci
Department of Drug Chemistry and Technologies, Faculty of Pharmacy, University Sapienza, Rome, Italy
J Control Release 147:127-35. 2010..Primary human lung fibroblast (HLF) cells were used for in vitro investigation of vesicle tolerability, carrier-cell interaction, intracellular drug ..
- The loss of circadian PAR bZip transcription factors results in epilepsyFrederic Gachon
Department of Molecular Biology, National Center of Competence Research Frontiers in Genetics, Sciences III, University of Geneva, CH 1211 Geneva 4, Switzerland
Genes Dev 18:1397-412. 2004DBP (albumin D-site-binding protein), HLF (hepatic leukemia factor), and TEF (thyrotroph embryonic factor) are the three members of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family...
- Interleukin-6 receptor superantagonist Sant7 inhibits TGF-beta-induced proliferation of human lung fibroblastsL Gallelli
Department of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, Italy
Cell Prolif 41:393-407. 2008..the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either IL-6 or TGF-beta1, the effects on phosphorylation of mitogen-activated protein kinases (MAPK) ..
- Expression of myelin basic protein (MBP) epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonal antibodiesR Chignola
Department of Pathology and Neurology Section, University of Verona, Italy
Clin Exp Immunol 122:429-36. 2000..30 MoAbs were selected on the basis of their different binding properties to: hLB-MBP, human lipid-free-MBP (hLF-MBP) and bovine lipid-free-MBP (bLF-MBP)...
- Frank S Lee; Fiscal Year: 2014..a family with hereditary erythrocytosis (increased red blood cell mass) due to a G537W missense mutation in the HIF2A gene, and another family with erythrocytosis due to a P317R missense mutation in the PHD2 gene...
- Yatrik M Shah; Fiscal Year: 2016..Hypoxia-inducible factors (HIFs) are heterodimeric transcription factors consisting of an alpha subunit (HIF1a or HIF2a) and beta subunit (aryl hydrocarbon nuclear translocator (ARNT))...
- THERAPY FOR GASTROINTESTINAL DISORDERSElizabeth Dial; Fiscal Year: 1999The main objective of this research proposal is to determine the efficacy of recombinant human lactoferrin (hLF) to treat gastrointestinal disorders induced by Helicobacter pylori infection or NSAID toxicity...
- Robert G Roeder; Fiscal Year: 2016..domain of E2A to the DNA- binding domain of pre-B-cell leukemia factor 1 (PBX1) or hepatic leukemia factor (HLF), generating fusion proteins (E2A-PBX1 and E2A-HLF) that result in pediatric acute lymphoblastic leukemias (ALL) ..
- Christopher A Pennell; Fiscal Year: 2015..Additionally, we will test the hypothesis that HIF2a is the molecular switch that induces expression of TLR2 ligands...
- Mechanism of Redox Regulation by Ape1/Ref-1Millie M Georgiadis; Fiscal Year: 2010..function that serves to reduce a number of transcription factors including AP-1 (Fos/Jun), HIF-1a, NF-kB, PAX, HLF, p53, and others (reviewed in 1)...
- Hamid M Said; Fiscal Year: 2016..studies, a yeast two-hybrid system was used to screen a human intestinal cDNA library and two proteins (PDZD11 and EPAS1) that interact with hSMVT were identified...
- Karen M Lyons; Fiscal Year: 2015..cartilage and nucleus pulposus (NP), and that they mediate their effects in part through regulation of HIF1a and HIF2a, and in part by acting as escorts/chaperones for ECM proteins...
- Analysis of Cell Death Regulation in C. elegansBarbara Conradt; Fiscal Year: 2012..We have also identified a three component pathway, the dnj- 11/MIDA1, ces-2/HLF, ces-1/Snail pathway, which is required for the asymmetric division of the NSM neuroblast and the exclusion of the ..
- E Jason Abel; Fiscal Year: 2016..Our project is significant because RCC progression is driven by HIF signaling and increased understanding of the NF-?B/HIF2? axis may allow development of new therapies for this incurable disease. ..
- KLF2 Mediated HIF-1 Regulation and Macrophage ActivationGANAPATI HOLANAGADDA MAHABALESHWAR; Fiscal Year: 2013..Recent studies have identified Hypoxia-inducible Factor 1alpha(HlF-1 alpha) as a key regulator of macrophage activation function...
- Hypoxia-inducible factors in alcoholic liver diseaseGyongyi Szabo; Fiscal Year: 2010..These hypotheses will be tested in the following Specific Aims: AIM1 To evaluate and compare the role of HIF1a and HIF2a in the pathogenesis of alcoholic steatosis by investigating a) Whether an upregulation of HIF1alpha and/or ..
- REGULATORY MECHANISMS IN SURFACTANT SYNTHESISCAROLE MENDELSON; Fiscal Year: 2002..the levels of the O2-regulated TFs NF-kappaB, its inhibitory partner IkappaB, HIF-1alpha and the related protein EPAS1/HLF in lung tissues of fetal baboons during development and after organ culture in 2 percent vs...
- PREDOCTORAL FELLOWSHIPS FOR STUDENTS WITH DISABILITIESShawn Terkhorn; Fiscal Year: 2006..proteins, the oxygen sensor prolyl hydroxylase (PHD) and the transcription factor, hypoxia inducible factor (HlF). Work in progress suggests that PHDs sense oxygen by modification of specific proline residues in HIF...
- PARTICULATE CHROMIUM TOXICITY AND CARCINOGENESISSteven Patierno; Fiscal Year: 2003..and soluble hexavalent chromium compounds with normal human small airway epithelial cells (HSAE) and fibroblasts (HLF), which are representative target cells for chromium toxicity and carcinogenesis...
- Radioprotective Role of Slug in Bone Marrow CellsWen Shu Wu; Fiscal Year: 2005..Slug was identified as a functional target of the E2A-HLF fusion oncoprotein in pro-B-cell acute leukemias, in which the E2A-HLF fusion gene was generated by the t(17:19)(..
- Hypoxic Sensing Transcription Factor EPAS1 in MiceJoseph Garcia; Fiscal Year: 2004..Endothelial PAS domain protein 1 (EPAS1), the second hypoxic-responsive member of the PAS family, is expressed at high levels in vascular endothelial ..
- Genetic Modifiers of p53Susan Evans; Fiscal Year: 2005..By genome-wide chromosomal mapping analyses, we identified loci containing modifiers of p53, mop1 and mop2, centromeric and telomeric to p53 on mouse chromosome 11 that causes embryonic lethality when combined with a p53 ..
- APOPTOSIS OF CARDIAC MUSCLE AS A TARGET FOR GENE THERAPYMichael Schneider; Fiscal Year: 2002..or baculovirus inhibitor of Apoptosis Proteins (IAPs); and a dominant- negative form of hepatic leukemia factor (HLF), a mammalian homologue for the transcription factor CES-2, a conserved cell death specification protein in ..
- Survival Signaling After Genotoxic InsultSusan Ceryak; Fiscal Year: 2009..pharmacological and genetic approaches, by using relevant model systems of human diploid lung fibroblasts (HLF), and human large airway epithelial cells (HLAE) and studying the involvement of key signaling components of the ..
- Hypoxia-inducible factor1a in multistage carcinogenesisJeffrey Arbeit; Fiscal Year: 2006..3.1. Create Kl4-E6HIF-1alpha or K14-E6:HlF-1alpha delta ODD double transgenic mice and determine alterations in the biology of skin and cervical ..
- BRAIN GENOMIC RESPONSE TO HYPOXIAFrank Sharp; Fiscal Year: 2002..Aim #3. Examine the genomic response to chronic hypoxia in HlF-1a knockout mice compared to wild type mice...
- Poor competition in hematopoietic stem/progenitor pools favors tumorigenesisJames DeGregori; Fiscal Year: 2009..To test our hypothesis, we will introduce the oncogenic Bcr-Abl and E2a-Hlf translocation products into mouse BM stem cells using retroviral transduction, and then reconstitute hematopoiesis ..
- Hypoxia-Inducible Factor 1 and Oxygen HomeostasisGregg Semenza; Fiscal Year: 2009..2. To investigate the role of HlF-1 in vascular progenitor cells...
- Synergistic activation of PTGS2 and CXCL8 by Ni and microbial stimuliKelly Brant; Fiscal Year: 2008..expression of PTGS2 and the immune-modulating chemokine CXCL8(IL-8) in cultured human lung fibroblast cells (HLF)...
- Mammalian Lactoferrin Receptors: Structure and FunctionBo Lonnerdal; Fiscal Year: 2007..Overall, our understanding of the physiological significance of Lf and its receptor will be increased. ..
- Signaling Pathways in Renal FibrogenesisH Schnaper; Fiscal Year: 2008..By elucidating the signaling events underlying kidney fibrosis we hope to better understand fibrogenic mechanisms and how to interrupt them. ..
- Particulate Matter/Mycoplasma Stress InteractionsJames Fabisiak; Fiscal Year: 2008..organism capable of latent/subclinical infection or colonization to deliberately infect human lung fibroblasts (HLF) in vitro in order to study the molecular and cellular basis for the synergistic interactions with residual oil fly ..
- A Novel Regulator of Oxygen HomeostasisFrank Lee; Fiscal Year: 2009..We anticipate that these studies will define a new pathway by which cells respond to hypoxia. ..
- Hypoxia inducible transcription in antitumor strategiesAndrew Kung; Fiscal Year: 2005..2. Search for small molecules that specifically perturb hypoxia- responsive transcription. 3. Attempt to create a mechanism-based imaging method for studying the effect of hypoxia-response inhibitors in animal tumors. ..
- ESTROGEN AND ENDOTHELIAL MIGRATION AND DIFFERENTIATIONH Schnaper; Fiscal Year: 2002..These studies will elucidate regulatory effects of estradiol on endothelial cell function. ..
- PATHOPHYSIOLOGY OF RENAL TUBULAR CELLSFrank Lee; Fiscal Year: 2002....
- MECHANISMS OF ENDOTHELIAL GAPDH REGULATION BY HYPOXIAKRISTA GRAVEN; Fiscal Year: 2002..abstract_text> ..
- PHARMACOGENOMIC STUDY OF ANTICONVULSANT THERAPYTHOMAS FERRARO; Fiscal Year: 2003....
- OXIDATIVE DNA DAMAGE AND REPAIR IN CNS CELLSMark Kelley; Fiscal Year: 2003..abstract_text> ..
- Functional Studies of VHL by Conditional Gene TargetingVolker Haase; Fiscal Year: 2003..Volker H. Haase, who is an Assistant Professor of Medicine in the University of Pennsylvania's Renal Division. Dr. Michael Madaio, Professor of Medicine, will function as Dr. Haase's mentor for this grant. ..
- VHL FUNCTION BY CONDITIONAL GENE TARGETINGVolker Haase; Fiscal Year: 2003..He is absolutely committed to a career centered on basic research in an academic Nephrology division applying the techniques learned during the award period. ..
- Renal Cell Injury in DiabetesKaren Block; Fiscal Year: 2008..Elucidating these events may allow future modulation of the enzymatic complex(es) to prevent oxidative stress implicated in cellular injury in diabetes. [unreadable] [unreadable]..
- TGF-beta and Cytoskeletal Signaling in Mesangial CellH Schnaper; Fiscal Year: 2005..These studies will determine whether increased cytoskeletal tension is required for RGTPase effects on collagen expression. ..
- Kidneys Fail:Translating basic mechanisms into therapiesH Schnaper; Fiscal Year: 2005..Our past experience is that these more social events are an important part of encouraging collaboration within and between the renal and non-renal communities. ..
- Molecular Mechanisms of the Hypoxic ResponseFrank Lee; Fiscal Year: 2006..We anticipate that the proposed studies will enlarge our understanding of both the molecular response to hypoxia and the role of a novel postranslational modification--proline hydroxylation. ..
- MOLECULAR MECHANISMS OF RENAL INJURYVolker Haase; Fiscal Year: 2007..Data generated from this grant would be used to guide a grant proposal for 5-year NIH funding. [unreadable] [unreadable] [unreadable]..
- Therapeutic/Mechanistic Role of Ape1 in Germ Cell TumorsMark Kelley; Fiscal Year: 2007..If any mutants are shown to sensitize the GCT cell lines to chemo-/IR agents, as expected, this will set the stage for future gene therapy approaches to sensitize GCT's to therapy. ..
- Hypoxic signaling in hepatocytesVolker Haase; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Imbalancing DNA BER to enhance Ovarian Tumor SensitivityMark Kelley; Fiscal Year: 2008..abstract_text> ..