EMP2

Summary

Gene Symbol: EMP2
Description: epithelial membrane protein 2
Alias: XMP, epithelial membrane protein 2
Species: human

Top Publications

  1. ncbi Epithelial membrane protein-2 is expressed in discrete anatomical regions of the eye
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA
    Exp Mol Pathol 74:106-12. 2003
  2. ncbi Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory, University of California, Los Angeles, CA 90095, USA
    Dev Biol 287:336-45. 2005
  3. ncbi Knockdown of the tetraspan protein epithelial membrane protein-2 inhibits implantation in the mouse
    Madhuri Wadehra
    Molecular Biology Institute, Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Dev Biol 292:430-41. 2006
  4. ncbi Epithelial membrane protein 2 modulates infectivity of Chlamydia muridarum (MoPn)
    Kaori Shimazaki
    Molecular Biology Institute, Los Angeles, CA 90095, USA
    Microbes Infect 9:1003-10. 2007
  5. pmc Steroid hormone regulation of EMP2 expression and localization in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Reprod Biol Endocrinol 6:15. 2008
  6. pmc FAK activation and the role of epithelial membrane protein 2 (EMP2) in collagen gel contraction
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:462-9. 2009
  7. pmc Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines
    Kaori Shimazaki
    Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Clin Cancer Res 14:7367-77. 2008
  8. pmc Functional consequences of interactions between FAK and epithelial membrane protein 2 (EMP2)
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:4949-56. 2009
  9. pmc Epithelial membrane protein-2 is a novel therapeutic target in ovarian cancer
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Clin Cancer Res 16:3954-63. 2010
  10. pmc Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e19945. 2011

Research Grants

  1. TRACY RUTH DANIELS-WELLS; Fiscal Year: 2014
  2. LIZBETH K HEDSTROM; Fiscal Year: 2016
  3. Friedhelm Hildebrandt; Fiscal Year: 2016
  4. Emp2: Potential Control Of Pathology In Retinal Pigment Epithelium
    Lynn Gordon; Fiscal Year: 2009
  5. IMP DEHYDROGENASE FROM PNEUMOCYSTIS AS A DRUG TARGET
    SHERRY QUEENER; Fiscal Year: 2000
  6. Madhuri Wadehra; Fiscal Year: 2016
  7. Novel Therapies to Prevent Blindness Caused by Proliferative Vitreoretinopathy
    Lynn K Gordon; Fiscal Year: 2013
  8. Targeting EMP2 in Endometrial Cancer
    Madhuri Wadehra; Fiscal Year: 2010
  9. BIOSYNTHESIS AND INTERCONVERSION OF PURINES
    JOSEPH GOTS; Fiscal Year: 1980
  10. MAPPING NUCLEOTIDE SITES OF E. COLI GMP SYNTHETASE
    OWEN MOE; Fiscal Year: 2000

Scientific Experts

Detail Information

Publications138 found, 100 shown here

  1. ncbi Epithelial membrane protein-2 is expressed in discrete anatomical regions of the eye
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA
    Exp Mol Pathol 74:106-12. 2003
    Epithelial membrane protein-2 (EMP2) is a member of the four transmembrane superfamily (TM4SF) and is thought to mediate trafficking of diverse proteins such as alpha6beta1 integrin and MHC class I to lipid raft microdomains...
  2. ncbi Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory, University of California, Los Angeles, CA 90095, USA
    Dev Biol 287:336-45. 2005
    The four-transmembrane protein epithelial membrane protein-2 (EMP2) was recently identified as an endometrial protein necessary for blastocyst implantation, but the mechanism of this role is uncertain...
  3. ncbi Knockdown of the tetraspan protein epithelial membrane protein-2 inhibits implantation in the mouse
    Madhuri Wadehra
    Molecular Biology Institute, Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Dev Biol 292:430-41. 2006
    ..In this study, we demonstrate that the tetraspan protein epithelial membrane protein-2 (EMP2) is involved in these molecular interactions during implantation...
  4. ncbi Epithelial membrane protein 2 modulates infectivity of Chlamydia muridarum (MoPn)
    Kaori Shimazaki
    Molecular Biology Institute, Los Angeles, CA 90095, USA
    Microbes Infect 9:1003-10. 2007
    ..Epithelial membrane protein-2 (EMP2), a 4-transmembrane protein, is highly expressed in epithelial cells in sites of chlamydial infections...
  5. pmc Steroid hormone regulation of EMP2 expression and localization in the endometrium
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Reprod Biol Endocrinol 6:15. 2008
    The tetraspan protein epithelial membrane protein-2 (EMP2), which mediates surface display of diverse proteins, is required for endometrial competence in blastocyst implantation, and is uniquely correlated with poor survival from ..
  6. pmc FAK activation and the role of epithelial membrane protein 2 (EMP2) in collagen gel contraction
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:462-9. 2009
    ..Since EMP2 is highly expressed in retinal pigment epithelium, this study investigates the role of EMP2 in collagen gel ..
  7. pmc Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines
    Kaori Shimazaki
    Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Clin Cancer Res 14:7367-77. 2008
    Endometrial cancer is the most common gynecologic malignancy. One promising biomarker is epithelial membrane protein 2 (EMP2), and its expression is an independent prognostic indicator for tumors with poor clinical outcome expression...
  8. pmc Functional consequences of interactions between FAK and epithelial membrane protein 2 (EMP2)
    Shawn A Morales
    Department of Pathology and Laboratory Medicine, University of California, Los Angeles, California, USA
    Invest Ophthalmol Vis Sci 50:4949-56. 2009
    Collagen gel contraction by ARPE-19 is controlled by epithelial membrane protein 2 (EMP2) through focal adhesion kinase (FAK) activation. The purpose of this study was to test the role of EMP2 in the cellular context of FAK activation.
  9. pmc Epithelial membrane protein-2 is a novel therapeutic target in ovarian cancer
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Clin Cancer Res 16:3954-63. 2010
    The tetraspan protein epithelial membrane protein-2 (EMP2) has been shown to regulate the surface display and signaling from select integrin pairs, and it was recently identified as a prognostic biomarker in human endometrial cancer...
  10. pmc Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e19945. 2011
    ..One recent biomarker strongly associated with disease progression and survival is epithelial membrane protein-2 (EMP2), a tetraspan protein known to associate with and modify surface expression of certain integrin isoforms...
  11. ncbi Tritrichomonas foetus: a strategy for structure-based inhibitor design of a protozoan inosine-5'-monophosphate dehydrogenase
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine 92697, USA
    Exp Parasitol 87:203-11. 1997
    ..The enzyme catalyzes the NAD-dependent oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), the committed step in guanosine monophosphate (GMP) biosynthesis...
  12. ncbi Asp338 controls hydride transfer in Escherichia coli IMP dehydrogenase
    K M Kerr
    Departments of Biochemistry and Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 39:9804-10. 2000
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD(+). This reaction involves the formation of a covalent adduct with an active site Cys...
  13. ncbi Crystal structure of Escherichia coli xanthine phosphoribosyltransferase
    S Vos
    Department of Biochemistry, The University of Queensland, Brisbane, Australia
    Biochemistry 36:4125-34. 1997
    ..1-pyrophosphate (PRib-PP) to the 6-oxopurine bases guanine, xanthine, and hypoxanthine to form GMP, XMP, and IMP, respectively...
  14. ncbi The first committed step reaction of caffeine biosynthesis: 7-methylxanthosine synthase is closely homologous to caffeine synthases in coffee (Coffea arabica L.)
    Kouichi Mizuno
    Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, 010 0195, Akita, Japan
    FEBS Lett 547:56-60. 2003
    ..CmXRS1 was specific for xanthosine and xanthosine 5'-monophosphate (XMP) could not be used as a substrate. The K(m) value for xanthosine was 73.7 microM...
  15. ncbi Chromosomal mapping of Tmp (Emp1), Xmp (Emp2), and Ymp (Emp3), genes encoding membrane proteins related to Pmp22
    I Ben-Porath
    Department of Genetics, Hebrew University, Jerusalem, Israel
    Genomics 49:443-7. 1998
    ..in the Charcot-Marie-Tooth neuropathy, and three novel genes: TMP, XMP, and YMP (HGMW-approved symbols EMP1, EMP2 and EMP3, respectively)...
  16. ncbi The tetraspan protein EMP2 increases surface expression of class I major histocompatibility complex proteins and susceptibility to CTL-mediated cell death
    Madhuri Wadehra
    Department of Pathology and Laboratory Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1722, USA
    Clin Immunol 107:129-36. 2003
    ..This study characterizes a new molecule affecting MHC1 expression and CTL cytotoxicity. Epithelial membrane protein 2 (EMP2) is a tetraspan protein recently identified for its role in suppressing B lymphoma ..
  17. ncbi Isolation and sequence of a cDNA encoding mouse IMP dehydrogenase
    A A Tiedeman
    Seattle Biomedical Research Institute, WA 98109 1651
    Gene 97:289-93. 1991
    Inosinic acid (IMP) dehydrogenase (IMPD) catalyzes the conversion of IMP to XMP as the first committed step in GMP biosynthesis de novo...
  18. pmc Active-site modification of native and mutant forms of inosine 5'-monophosphate dehydrogenase from Escherichia coli K12
    H J Gilbert
    Biochem J 191:533-41. 1980
    ..Inactivation by Cl-IMP was retarded by ligands that bind at the IMP-binding site. Their effectiveness was IMP>XMP>GMP>>AMP. NAD(+) did not protect the enzyme from modification...
  19. ncbi IMP dehydrogenase: mechanism of action and inhibition
    L Hedstrom
    MS 009, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    Curr Med Chem 6:545-60. 1999
    Inosine monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP with the concomitant reduction of NAD to NADH. This reaction is the rate-limiting step in guanine nucleotide biosynthesis...
  20. ncbi Xanthosine and xanthine. Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems
    Gerasim Stoychev
    Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Poland
    Eur J Biochem 269:4048-57. 2002
    ..Attention is directed to the fact that the structural properties of the neutral and ionic forms of XMP, Xao and Xan are also of key importance in many other enzyme systems, such as IMP dehydrogenase, some nucleic acid ..
  21. pmc De novo GMP synthesis is required for axon guidance in Drosophila
    Hong Long
    McGill Centre for Research in Neuroscience and Department of Neurology and Neurosurgery, McGill University Health Centre, Montreal, Quebec H3G 1A4, Canada
    Genetics 172:1633-42. 2006
    ..were observed when the raspberry (ras) gene, encoding for inosine monophosphate dehydrogenase catalyzing the IMP-to-XMP conversion in GMP de novo synthesis, was mutated...
  22. doi Faster exact maximum parsimony search with XMP
    W Timothy J White
    Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand
    Bioinformatics 27:1359-67. 2011
    ..This is, in part, because currently available programs fail to take advantage of parallelism...
  23. pmc Bacillus anthracis inosine 5'-monophosphate dehydrogenase in action: the first bacterial series of structures of phosphate ion-, substrate-, and product-bound complexes
    Magdalena Makowska-Grzyska
    Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637, USA
    Biochemistry 51:6148-63. 2012
    ..form and in complex with its substrate, inosine 5'-monophosphate (IMP), and product, xanthosine 5'-monophosphate (XMP). This is the first example of a bacterial IMPDH in more than one state from the same organism...
  24. pmc Epithelial membrane protein 2 controls VEGF expression in ARPE-19 cells
    Shawn A Morales
    Department of Ophthalmology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California 90095, USA
    Invest Ophthalmol Vis Sci 54:2367-72. 2013
    ..b>EMP2 is highly expressed in the RPE and has been shown to regulate FAK activation, which is implicated in VEGF ..
  25. doi Density functional theory study of the interaction of vinyl radical, ethyne, and ethene with benzene, aimed to define an affordable computational level to investigate stability trends in large van der Waals complexes
    Andrea Maranzana
    Dipartimento di Chimica, Università di Torino, Corso Massimo D Azeglio 48, I 10125 Torino, Italy
    J Chem Phys 139:244306. 2013
    ..3) Based on an additivity assumption of (i) the estimated MP2 energy at the complete basis set limit [EMP2/CBS] and (ii) the higher-order correlation energy effects in passing from MP2 to CCSD(T) at the aug-cc-pVTZ basis ..
  26. ncbi The tetraspan protein epithelial membrane protein-2 interacts with beta1 integrins and regulates adhesion
    Madhuri Wadehra
    Molecular Biology Institute, The David Geffen School of Medicine at UCLA and Jonsson Comprehensive Cancer Center, 108ee Le Conte Avenue, Los Angeles, CA 90095, USA
    J Biol Chem 277:41094-100. 2002
    ..Epithelial membrane protein-2 (EMP2), a GAS3/PMP22 family member, was recently identified as a putative tumor suppressor gene...
  27. pmc The tetraspan protein EMP2 modulates the surface expression of caveolins and glycosylphosphatidyl inositol-linked proteins
    Madhuri Wadehra
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Mol Biol Cell 15:2073-83. 2004
    ..Epithelial membrane protein-2 (EMP2), a member of the four-transmembrane superfamily, facilitates plasma membrane delivery of certain integrins...
  28. ncbi Determination of inosine monophosphate dehydrogenase activity in human CD4+ cells isolated from whole blood during mycophenolic acid therapy
    Nils Tore Vethe
    Department of Medical Biochemistry, Rikshospitalet University Hospital, Oslo, Norway
    Ther Drug Monit 28:608-13. 2006
    ..NAD; co-factor) were added to cell lysates, and IMPDH activity was quantified as the xanthosine 5'-monophosphate (XMP) production rate (pmol/10 cells/min) determined by liquid chromatography after hydrolytic cleavage to xanthine...
  29. doi Achieving the CCSD(T) Basis-Set Limit in Sizable Molecular Clusters: Counterpoise Corrections for the Many-Body Expansion
    Ryan M Richard
    Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, United States
    J Phys Chem Lett 4:2674-80. 2013
    ..A triples correction, δCCSD(T) = ECCSD(T) - EMP2, can be estimated accurately and efficiently as well...
  30. ncbi Action of the active metabolites of tiazofurin and ribavirin on purified IMP dehydrogenase
    Y Yamada
    Laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis 46223
    Biochemistry 27:2193-6. 1988
    ..where IMP binds to the enzyme first, followed by NAD+; NADH dissociates from the ternary complex first and then XMP is released...
  31. ncbi Nucleotide sequence of the guaA gene encoding GMP synthetase of Escherichia coli K12
    A A Tiedeman
    J Biol Chem 260:8676-9. 1985
    ..3.4.1), a glutamine amido-transferase encoded by the guaA gene, catalyzes the synthesis of GMP from XMP. The guaA gene was subcloned from the Clarke and Carbon (Clarke, L., and Carbon, J...
  32. ncbi Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase
    A Jong
    Department of Pediatrics and Microbiology, University of Southern California School of Medicine, Los Angeles 90033
    Arch Biochem Biophys 304:197-204. 1993
    ..dUMP as phosphate acceptors with high activity; to a lesser extent, it can also use IMP, GMP, dGMP, 5-iodo-dUMP, XMP, and dTMP as substrates...
  33. ncbi Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase
    S Vos
    Centre for Protein Structure, Function and Engineering, University of Queensland, Australia
    J Struct Biol 116:330-4. 1996
    ..4.2.22) from Escherichia coli is a purine salvage enzyme which synthesizes the nucleotides GMP, XMP, and IMP...
  34. ncbi Isolation and characterization of three novel serine protease genes from Xenopus laevis
    K Yamada
    Graduate School of Human Informatics, Nagoya University, Furo cho, Chikusa ku, 464 8601, Nagoya, Japan
    Gene 252:209-16. 2000
    ..Xesp-1 is likely to be a secreted protein that functions in the extracellular space. Xesp-2 and XMP-SP1 are likely to be type II membrane proteases with multidomain structures...
  35. ncbi Metabolic fate of AMP, IMP, GMP and XMP in the cytosol of rat brain: an experimental and theoretical analysis
    A Torrecilla
    Departamento de Bioquimica, Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Facultad de Medicina, Madrid, Spain
    J Neurochem 76:1291-307. 2001
    A systematic study of the metabolic fate of AMP, IMP, GMP and XMP (NMP) in the presence of cytosol from rat brain is here presented; the kinetics of both disappearance of NMP, and appearance of their degradation products was followed by ..
  36. ncbi A mutational analysis of the active site of human type II inosine 5'-monophosphate dehydrogenase
    Olga Futer
    Vertex Pharmaceuticals, Inc, 130 Waverly Street, Cambridge, MA 02139 4242, USA
    Biochim Biophys Acta 1594:27-39. 2002
    The oxidation of IMP to XMP is the rate-limiting step in the de novo synthesis of guanine ribonucleotides. This NAD-dependent reaction is catalyzed by the enzyme inosine monophosphate dehydrogenase (IMPDH)...
  37. ncbi Crystal structure of Tritrichomonas foetus inosine monophosphate dehydrogenase in complex with the inhibitor ribavirin monophosphate reveals a catalysis-dependent ion-binding site
    Glen L Prosise
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 3900, USA
    J Biol Chem 277:50654-9. 2002
    ..The enzyme binds IMP and the cofactor NAD(+) in random order, IMP is converted to XMP, NAD(+) is reduced to NADH, and finally, NADH and then XMP are released sequentially...
  38. pmc Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis
    Suneng Fu
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 14:3119-32. 2002
    ..Recessive Mutator transposon mutations at the maize empty pericarp2 (emp2) locus led to dramatically increased expression of heat shock genes, retarded embryo development, and early-stage ..
  39. ncbi Structural insights into the avian AICAR transformylase mechanism
    Dennis W Wolan
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochemistry 41:15505-13. 2002
    ..was determined as a complex with the AICAR Tfase substrate AICAR, as well as with an IMP cyclohydrolase inhibitor, XMP, to 1.93 A resolution...
  40. ncbi Induction of apoptosis in IL-3-dependent hematopoietic cell lines by guanine nucleotide depletion
    Jing Jin Gu
    Lineberger Comprehensive Cancer Center, Department of Pharmacology, University of North Carolina at Chapel Hill, 27599, USA
    Blood 101:4958-65. 2003
    ..dehydrogenase (IMPDH) is a rate-limiting enzyme that catalyzes the conversion of IMP to xanthosine monophosphate (XMP) at the branch point of purine nucleotide biosynthesis, leading to the generation of guanine nucleotides...
  41. pmc Clonal mosaic analysis of EMPTY PERICARP2 reveals nonredundant functions of the duplicated HEAT SHOCK FACTOR BINDING PROTEINs during maize shoot development
    Suneng Fu
    Plant Biology Department, University of Georgia, Athens, Georgia 30602, USA
    Genetics 167:1381-94. 2004
    The paralogous maize proteins EMPTY PERICARP2 (EMP2) and HEAT SHOCK FACTOR BINDING PROTEIN2 (HSBP2) each contain a single recognizable motif: the coiled-coil domain...
  42. ncbi Evidence for the involvement of cytosolic 5'-nucleotidase (cN-II) in the synthesis of guanine nucleotides from xanthosine
    Catia Barsotti
    Department of Physiology and Biochemistry, University of Pisa, Via San Zeno 51, 56127 Pisa, Italy
    J Biol Chem 280:13465-9. 2005
    ..In rat brain extracts and in intact LoVo cells, xanthosine is salvaged to XMP via the phosphotransferase activity of cytosolic 5'-nucleotidase...
  43. ncbi Estimated MP2 and CCSD(T) interaction energies of n-alkane dimers at the basis set limit: comparison of the methods of Helgaker et al. and Feller
    Seiji Tsuzuki
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8568, Japan
    J Chem Phys 124:114304. 2006
    ..The estimated MP2 interaction energies of methane, ethane, and propane dimers at the basis set limit [EMP2(limit)] by the method of Helgaker et al. [J. Chem. Phys...
  44. doi Biochemical characterization of recombinant guaA-encoded guanosine monophosphate synthetase (EC 6.3.5.2) from Mycobacterium tuberculosis H37Rv strain
    Tathyana Mar A Franco
    Instituto Nacional de Ciência e Tecnologia em Tuberculose INCT TB, Centro de Pesquisas em Biologia Molecular e Funcional CPBMF, Pontificia Universidade Catolica do Rio Grande do Sul PUCRS, Porto Alegre, RS, Brazil
    Arch Biochem Biophys 517:1-11. 2012
    ..The conversion of XMP to GMP is catalyzed by guaA-encoded GMP synthetase (GMPS), and deletions in the Shiguella flexneri guaBA operon led ..
  45. doi Synthesis of 9-phosphonoalkyl and 9-phosphonoalkoxyalkyl purines: evaluation of their ability to act as inhibitors of Plasmodium falciparum, Plasmodium vivax and human hypoxanthine-guanine-(xanthine) phosphoribosyltransferases
    Michal Cesnek
    Institute of Organic Chemistry and Biochemistry, Academy of Sciences of Czech Republic, Flemingovo nam 2, 166 10 Prague 6, Czech Republic
    Bioorg Med Chem 20:1076-89. 2012
    ..phosphoribosyltransferase [HG(X)PRT], catalyses the synthesis of the purine nucleoside monophosphates, IMP, GMP or XMP essential for DNA/RNA production...
  46. pmc Positron emission tomography imaging of endometrial cancer using engineered anti-EMP2 antibody fragments
    Maoyong Fu
    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Mol Imaging Biol 15:68-78. 2013
    As imaging of the cell surface tetraspan protein epithelial membrane protein-2 (EMP2) expression in malignant tumors may provide important prognostic and predictive diagnostic information, the goal of this study is to determine if ..
  47. pmc Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase from Thermotoga maritima
    Khaldeyah Awwad
    Chemistry and Biochemistry, California State University East Bay, 25800 Carlos Bee Boulevard, Hayward, CA 94542, USA
    Acta Crystallogr D Biol Crystallogr 69:184-93. 2013
    ..triphosphate (XTP) into inosine monophosphate (IMP), deoxyinosine monophosphate (IMP) and xanthosine monophosphate (XMP), respectively. The k(cat)/K(m) values determined at 323 and 353 K fall between 1.31 × 10(4) and 7...
  48. doi Substrate specificity and oligomerization of human GMP synthetase
    Martin Welin
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 17177 Stockholm, Sweden
    J Mol Biol 425:4323-33. 2013
    ..domain generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate (XMP) to form GMP...
  49. pmc Rationale and preclinical efficacy of a novel anti-EMP2 antibody for the treatment of invasive breast cancer
    Maoyong Fu
    Authors Affiliations Departments of Pathology and Laboratory Medicine, Surgery, Division of Surgical Oncology, Ophthalmology, and Microbiology, Immunology, and Molecular Genetics, and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA Department of Surgery, Greater Los Angeles Veterans Affairs Healthcare System Department of Pathology, Charles Drew University, Los Angeles, California Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York and Institute of Experimental Biology and Medicine, University of Buenos Aires, Buenos Aires, Argentina
    Mol Cancer Ther 13:902-15. 2014
    ..we show evidence for a novel target in human breast cancer, the tetraspan protein epithelial membrane protein-2 (EMP2)...
  50. pmc Epithelial membrane protein-2 (EMP2) activates Src protein and is a novel therapeutic target for glioblastoma
    Yu Qin
    From the Departments of Ophthalmology and
    J Biol Chem 289:13974-85. 2014
    ..In this study, we have identified the tetraspan protein epithelial membrane protein-2 (EMP2) as a potential target for glioblastoma (GBM) killing...
  51. pmc Mutations in EMP2 cause childhood-onset nephrotic syndrome
    Heon Yung Gee
    Division of Nephrology, Department of Medicine, Boston Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Am J Hum Genet 94:884-90. 2014
    ..We thereby detected biallelic mutations in EMP2 (epithelial membrane protein 2) in four individuals from three unrelated families affected by SRNS or SSNS...
  52. pmc Recipient pretransplant inosine monophosphate dehydrogenase activity in nonmyeloablative hematopoietic cell transplantation
    Meagan J Bemer
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington School of Pharmacy, University of Washington, Seattle, Washington
    Biol Blood Marrow Transplant 20:1544-52. 2014
    ..of guanosine nucleotides and catalyzes the oxidation of inosine 5'-monophosphate to xanthosine 5'-monophosphate (XMP)...
  53. pmc A novel cofactor-binding mode in bacterial IMP dehydrogenases explains inhibitor selectivity
    Magdalena Makowska-Grzyska
    From the Center for Structural Genomics of Infectious Diseases, Computational Institute, University of Chicago, Chicago, Illinois 60637
    J Biol Chem 290:5893-911. 2015
    ..IMPDH catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD(+), which is the pivotal step in the biosynthesis of guanine nucleotides...
  54. doi Sensitive and validated LC-MS/MS methods to evaluate mycophenolic acid pharmacokinetics and pharmacodynamics in hematopoietic stem cell transplant patients
    Misaki Kawanishi
    Department of Clinical Pharmacy and Education, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan
    Biomed Chromatogr 29:1309-16. 2015
    ..dehydrogenase activity was calculated by measuring concentrations of produced xanthosine-5'-monophosphate (XMP) and intracellular adenosine-5'-monophosphate after incubation of peripheral blood mononuclear cell (PBMC) lysates...
  55. pmc Regulation of FAK Activity by Tetraspan Proteins: Potential Clinical Implications in Cancer
    Yu Qin
    Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA
    Crit Rev Oncog 20:391-405. 2015
    ..include select tetraspanins such as CD151 and CD9 as well as the GAS3 family members epithelial membrane protein-2 (EMP2) and peripheral myelin protein-22 (PMP22)...
  56. pmc Review of the GAS3 Family of Proteins and their Relevance to Cancer
    Negin Ashki
    Department of Ophthalmology, Jules Stein Eye Institute, Los Angeles, CA
    Crit Rev Oncog 20:435-47. 2015
    ..Finally, the impact of targeting select members of the family such as PMP22 and EMP2 is discussed.
  57. pmc Epithelial Membrane Protein-2 in Human Proliferative Vitreoretinopathy and Epiretinal Membranes
    David G Telander
    Department of Ophthalmology University of California, Davis, Sacramento, California, United States
    Invest Ophthalmol Vis Sci 57:3112-7. 2016
    To determine the level of epithelial membrane protein-2 (EMP2) expression in preretinal membranes from surgical patients with proliferative vitreoretinopathy (PVR) or epiretinal membranes (ERMs)...
  58. doi Epithelial Membrane Protein 2 and β1 integrin signaling regulate APC-mediated processes
    Alyssa C Lesko
    Department of Biological Science, Harper Cancer Research Institute, University of Notre Dame, United States
    Exp Cell Res 350:190-198. 2017
    ..cyst size and inverts polarity independent of Wnt signaling, and upregulates the tetraspan protein, Epithelial Membrane Protein 2 (EMP2). Herein, we show that APC loss increases β1 integrin expression and migration of MDCK cells...
  59. doi Ultrafast Electronic Deactivation Dynamics of Xanthosine Monophosphate
    Katharina Röttger
    Institute of Physical Chemistry, Christian Albrechts University Kiel, Olshausenstr 40, 24098 Kiel, Germany
    Molecules 22:. 2017
    ..on the excited-state dynamics of the rare nucleotide xanthosine monophosphate as a function of deprotonation state (XMP vs...
  60. doi Determination of inosine 5'-monophosphate dehydrogenase activity in red blood cells of thiopurine-treated patients using HPLC
    Audrey Beringer
    Universite de Lyon, Universite Lyon 1, UMR CNRS 5305, Pharmacie Clinique, Pharmacocinétique et Évaluation du Médicament, Lyon, France Hospices civils de Lyon, Groupement Hospitalier Edouard Herriot, Laboratoire de Biologie Médicale Multi Sites du CHU de Lyon, unité de Pharmacocinétique Clinique, Lyon, France
    J Chromatogr B Analyt Technol Biomed Life Sci . 2017
    ..activity was evaluated by enzymatic conversion of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP)...
  61. ncbi Human GMP synthetase
    T Page
    Int J Biochem 16:117-20. 1984
    ..The Km values were determined to be 4.9 microM for XMP, 270 microM for ATP, and 340 microM for glutamine...
  62. ncbi The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog
    K M Kerr
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Biochemistry 36:13365-73. 1997
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD+; the enzyme is activated by K+. This reaction is the rate-limiting step in de novo guanine nucleotide biosynthesis...
  63. ncbi Crystal structure of a ternary complex of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase: NAD+ orients the active site loop for catalysis
    Lu Gan
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 41:13309-17. 2002
    Inosine 5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP with the reduction of NAD(+), which is the rate-limiting step in the biosynthesis of guanine nucleotides. IMPDH is a promising target for chemotherapy...
  64. ncbi The immunosuppressive agent mizoribine monophosphate forms a transition state analogue complex with inosine monophosphate dehydrogenase
    Lu Gan
    Department of Biochemistry and Chemistry and the Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 42:857-63. 2003
    ..This enzyme catalyzes the oxidation of IMP to XMP with the concomitant reduction of NAD via a covalent intermediate at Cys319 (E-XMP)...
  65. ncbi Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism
    Glen L Prosise
    Department of Molecular Biology and Biochemistry University of California, Irvine, CA 92697 3900 USA
    J Mol Biol 326:517-27. 2003
    ..foetus have been solved: with its substrate IMP, IMP and the inhibitor mycophenolic acid (MPA), the product XMP with MPA, and XMP with the cofactor NAD(+). In addition, a potassium ion has been located at the dimer interface...
  66. ncbi Structural properties of the neutral and monoanionic forms of xanthosine, highly relevant to their substrate properties with various enzyme systems
    Jarosław Poznański
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Nucleosides Nucleotides Nucleic Acids 22:249-63. 2003
    ..These findings are highly relevant to the modes of binding, and to the substrate properties, of Xan, Xao and its 5'-phosphate (XMP) in numerous enzyme systems, hitherto virtually ignored, and illustrated by several concrete examples.
  67. ncbi Substitution of the conserved Arg-Tyr dyad selectively disrupts the hydrolysis phase of the IMP dehydrogenase reaction
    Yollete V Guillen Schlippe
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 43:4511-21. 2004
    Inosine 5'-monophosphate dehydrogenase (IMPDH) catalyzes the oxidation of IMP to XMP via the covalent E-XMP* intermediate (E-XMP*), with the concomitant reduction of NAD(+)...
  68. ncbi Acid-base properties of xanthosine 5'-monophosphate (XMP) and of some related nucleobase derivatives in aqueous solution: micro acidity constant evaluations of the (N1)H versus the (N3)H deprotonation ambiguity
    Salah S Massoud
    Departement Chemie, Anorganische Chemie, Universitat Basel, Spitalstrasse 51, 4056 Basel, Switzerland
    Chemistry 10:5129-37. 2004
    The first acidity constant of fully protonated xanthosine 5'-monophosphate, that is, of H3(XMP)+, was estimated by means of a micro acidity constant scheme and the following three deprotonations of the H2(XMP)+/- (pKa=0.97), H(XMP)- (5...
  69. ncbi Validation and application of a high-performance liquid chromatographic-based assay for determination of the inosine 5'-monophosphate dehydrogenase activity in erythrocytes
    Philipe N Khalil
    Department of Surgery, Downtown Medical Centre, Ludwig Maximilians University, D 80336 Munich, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 842:1-7. 2006
    ..erythrocyte separation/haemolysis and assay conditions and a distinct segregation of xanthosine 5'-monophosphate (XMP) from the clear supernatant after precipitation...
  70. ncbi Kinetic and biochemical characterization of Plasmodium falciparum GMP synthetase
    Javaid Yousuf Bhat
    Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore 560064, India
    Biochem J 409:263-73. 2008
    ..GMPS, a G-type amidotransferase, catalyses the amination of XMP to GMP with the reaction occurring in two domains, the GAT (glutamine amidotransferase) and ATPPase (ATP ..
  71. doi Substrate-induced conformational changes in Plasmodium falciparum guanosine monophosphate synthetase
    Javaid Y Bhat
    Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
    FEBS J 278:3756-68. 2011
    ..amidotransferase that incorporates ammonia derived from glutamine into the nucleotide xanthosine 5'-monophosphate (XMP) to form guanosine 5'-monophosphate (GMP)...
  72. pmc Defects in purine nucleotide metabolism lead to substantial incorporation of xanthine and hypoxanthine into DNA and RNA
    Bo Pang
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:2319-24. 2012
    ..we observed large increases (up to 600-fold) in hypoxanthine in both DNA and RNA in cells unable to convert IMP to XMP or AMP (IMP dehydrogenase, guaB; adenylosuccinate synthetase, purA, and ADE12), and unable to remove dITP/ITP and ..
  73. pmc Identification of novel Mt-Guab2 inhibitor series active against M. tuberculosis
    Veeraraghavan Usha
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom
    PLoS ONE 7:e33886. 2012
    ..IMPDH exclusively catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) in the presence of the cofactor nicotinamide adenine dinucleotide (NAD(+))...
  74. pmc EMP2 regulates angiogenesis in endometrial cancer cells through induction of VEGF
    L K Gordon
    Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Oncogene 32:5369-76. 2013
    ..In this study, we define a novel function for epithelial membrane protein-2 (EMP2) in the control of angiogenesis...
  75. pmc Substrate activation and conformational dynamics of guanosine 5'-monophosphate synthetase
    Justin C Oliver
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, United States
    Biochemistry 52:5225-35. 2013
    ..responsible for the last step in the guanosine branch of the pathway, the amination of xanthosine 5'-monophosphate (XMP)...
  76. doi IPH-926 lobular breast cancer cells are triple-negative but their microarray profile uncovers a luminal subtype
    Matthias Christgen
    Institute of Pathology, Hannover Medical School, Hannover, Germany
    Cancer Sci 104:1726-30. 2013
    ..Quantitative real-time RT-PCR confirmed enhanced expression of luminal marker genes (AGR2, CLU, CA12, EMP2, CLDN3) and low or absent expression of basal marker genes (KRT5, CD44, CAV1, VIM)...
  77. ncbi Positional isotope exchange and kinetic experiments with Escherichia coli guanosine-5'-monophosphate synthetase
    W von der Saal
    Biochemistry 24:5343-50. 1985
    ..The initial velocity patterns of MgATP, XMP, and either NH3 or glutamine (as nitrogen source) were consistent with the ordered addition of MgATP followed by ..
  78. ncbi Differential effects of bactericidal/permeability-increasing protein (BPI) analogues on retinal neovascularization and retinal pericyte growth
    Ravi K Rauniyar
    Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    Invest Ophthalmol Vis Sci 43:503-9. 2002
    ..retinopathies, the antiangiogenic activity of a human recombinant 21-kDa modified N-terminal fragment of BPI (rBPI(21)), which has the biological properties of the holoprotein, and a peptidomimetic (XMP.Z) derived from BPI were examined.
  79. ncbi Guanidine derivatives rescue the Arg418Ala mutation of Tritrichomonas foetus IMP dehydrogenase
    Yollete V Guillen Schlippe
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA
    Biochemistry 44:16695-700. 2005
    IMP dehydrogenase (IMPDH) catalyzes the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) and the reduction of NAD(+)...
  80. pmc Loss of epithelial membrane protein-2 expression confers an independent prognosticator in nasopharyngeal carcinoma: a cohort study
    Yi Hsien Chen
    Department of Emergency Medicine, Chi Mei Medical Center, Tainan, Taiwan
    BMJ Open 2:e000900. 2012
    To evaluate the expression of epithelial membrane protein-2 (EMP2) protein and its clinicopathological associations in patients with nasopharyngeal carcinoma.
  81. ncbi Analysis of purine nucleotides in lymphocytes from healthy subjects and AIDS patients
    A Tabucchi
    Istituto di Biochimica e di Enzimologia, , Italy
    Biomed Pharmacother 46:25-9. 1992
    The most important purine nucleotides (NAD, AMP, IMP, GMP, XMP, ADP, ATP, GDP, GTP) were analyzed by HPLC in the lymphocytes of healthy subjects and HIV-1 seropositive patients at different stages of the disease (ARC-AIDS)...
  82. ncbi A novel mechanism of mycophenolic acid resistance in the protozoan parasite Tritrichomonas foetus
    L Hedstrom
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143
    Biochem Pharmacol 39:151-60. 1990
    ..Xanthine incorporation via XMP provided an IMP dehydrogenase independent route to guanine nucleotides that would enable the parasite to become ..
  83. ncbi Mycophenolic acid and thiazole adenine dinucleotide inhibition of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase: implications on enzyme mechanism
    L Hedstrom
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143
    Biochemistry 29:849-54. 1990
    ..dehydrogenase (IMPDH) catalyzes the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) with the conversion of NAD to NADH...
  84. ncbi Increased activity, amount, and altered kinetic properties of IMP dehydrogenase from mycophenolic acid-resistant neuroblastoma cells
    S D Hodges
    Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada
    J Biol Chem 264:18137-41. 1989
    ..4 nM to 3.4 microM for the enzyme from NB versus NB-Myco cells, and the Ki for XMP was increased 4-fold from 78 to 336 microM...
  85. ncbi Dual effects of pyrazofurin and 3-deazauridine upon pyrimidine and purine biosynthesis in mouse L1210 leukemia
    M E Sant
    Department of Biochemistry, University of Sydney, Australia
    Cancer Res 49:2645-50. 1989
    ..concentrations of N-succino-AICAR (SAICAR), AICAR, 5-formamidoimidazole-4-carboxamide ribotide (FAICAR), IMP, XMP, and GMP at later times indicate that AICAR transformylase is not significantly inhibited in cultured cells; rather ..
  86. ncbi Purification and characterization of GMP synthetase from Yoshida sarcoma ascites cells
    K Hirai
    Division of Protein Metabolism, Osaka University
    J Biochem 102:893-902. 1987
    ..Its isoelectric point was estimated to be 5.6. The Km values of this enzyme for XMP, ATP, and glutamine were calculated to be 4.6, 120, and 300 microM, respectively...
  87. ncbi Allelic differences in the VHOx-1 gene explain the absence of a B cell clonal dominance in the primary response of C57BL/6 mice to phthalate
    D B Winter
    Department of Molecular Immunology, Rosewell Park Cancer Institute, Unit of the New York State Department of Health, Buffalo 14263, USA
    J Immunol 155:2445-52. 1995
    ..A highly conserved Id (CRIXmp-1) associated with the murine (BALB/c) humoral immune response to the hapten phthalate (Xmp) is conspicuously absent in C57BL/6 mice...
  88. ncbi Biochemical characterization of human GMP synthetase
    J Nakamura
    Institute of Biochemistry and Cell Biology, Syntex Discovery Research, Palo Alto, California 94304, USA
    J Biol Chem 270:7347-53. 1995
    ..Interaction of GMP synthetase with xanthosine 5'-monophosphate (XMP), a substrate, exhibits sigmoidal kinetics with a Hill coefficient of 1.48 +/- 0.07...
  89. ncbi Preliminary X-ray analysis of Escherichia coli GMP synthetase: determination of anomalous scattering factors for a cysteinyl mercury derivative
    J J Tesmer
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    Proteins 18:394-403. 1994
    ..GMPS catalyzes the conversion of XMP to GMP in the final step of de novo guanine nucleotide biosynthesis, and is a member of the glutamine ..
  90. ncbi Inactivation of inosine 5'-monophosphate dehydrogenase by the antiviral agent 5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide 5'-monophosphate
    W Wang
    Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Biochemistry 35:95-101. 1996
    ..IMPDH converts inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) with concomitant conversion of NAD+ to NADH...
  91. ncbi Inhibition of IMPDH by mycophenolic acid: dissection of forward and reverse pathways using capillary electrophoresis
    M A Fleming
    Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139 4211, USA
    Biochemistry 35:6990-7. 1996
    ..Slow inhibition, without substrate turnover, was achieved by incubating free enzyme with excess XMP and MPA...
  92. ncbi Characterization of a tumor-associated gene, a member of a novel family of genes encoding membrane glycoproteins
    I Ben-Porath
    Department of Genetics, Hebrew University of Jerusalem, Givat Ram, Israel
    Gene 183:69-75. 1996
    ..In our search for human sequences homologous to TMP and PMP22, we identified two new genes which we have named XMP and YMP...
  93. ncbi High level expression of XMP aminase in Escherichia coli and its application for the industrial production of 5'-guanylic acid
    T Fujio
    Planning and Development Department Food, Liquor and Food Division, Kyowa Hakko Kogya Co, Ltd, Tokyo, Japan
    Biosci Biotechnol Biochem 61:840-5. 1997
    To improve the efficiency of the enzymatic conversion of 5'-xanthylic acid (XMP) to 5'-guanylic acid (GMP), we attempted to increase the activity of the conversion enzyme, XMP aminase (GMP synthetase) encoded by the guaA gene in ..
  94. ncbi Crystal structure of Tritrichomonas foetus inosine-5'-monophosphate dehydrogenase and the enzyme-product complex
    F G Whitby
    Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, 94143 0446, USA
    Biochemistry 36:10666-74. 1997
    ..The enzyme catalyzes the oxidation of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), the committed step in de novo guanosine monophosphate (GMP) biosynthesis...
  95. ncbi Metabolic effects of thiopurine derivatives against human CCRF-CEM leukaemia cells
    R Z Shi
    Department of Biochemistry, University of Sydney, NSW, Australia
    Int J Biochem Cell Biol 30:885-95. 1998
    ....
  96. ncbi N2-hydroxyguanosine 5'-monophosphate is a time-dependent inhibitor of Escherichia coli guanosine monophosphate synthetase
    M L Deras
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907 1333, USA
    Biochemistry 38:303-10. 1999
    ..One untested hypothesis was that hydroxylamine serves as a substrate and intercepts a xanthosine 5'-monophosphate- (XMP-) adenylate intermediate in the enzyme active site...
  97. ncbi Acid-base catalysis in the chemical mechanism of inosine monophosphate dehydrogenase
    G D Markham
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Biochemistry 38:4433-40. 1999
    Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the K+-dependent reaction IMP + NAD + H2O --> XMP + NADH + H+ which is the rate-limiting step in guanine nucleotide biosynthesis...
  98. ncbi Regional localization of the human epithelial membrane protein genes 1, 2, and 3 (EMP1, EMP2, EMP3) to 12p12.3, 16p13.2, and 19q13.3
    T Liehr
    Institute of Human Genetics, Schwabachanlage 10, Erlangen, D 91054, Germany
    Genomics 58:106-8. 1999
  99. ncbi Structure-based identification of a novel NTPase from Methanococcus jannaschii
    K Y Hwang
    Structural Biology Center, Korea Institute of Science and Technology, Seoul, South Korea
    Nat Struct Biol 6:691-6. 1999
    ..analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
  100. pmc Broad-spectrum antiviral activity of the IMP dehydrogenase inhibitor VX-497: a comparison with ribavirin and demonstration of antiviral additivity with alpha interferon
    W Markland
    Vertex Pharmaceuticals Inc, Cambridge, Massachusetts 02139 4242, USA
    Antimicrob Agents Chemother 44:859-66. 2000
    ..catalyzes an essential step in the de novo biosynthesis of guanine nucleotides, namely, the conversion of IMP to XMP. The major event occurring in cells exposed to competitive IMPDH inhibitors such as ribavirin or uncompetitive ..
  101. ncbi Crystal structure at 2.4 A resolution of Borrelia burgdorferi inosine 5'-monophosphate dehydrogenase: evidence of a substrate-induced hinged-lid motion by loop 6
    F M McMillan
    The Rosenstiel Basic Medical Sciences Research Center and Department of Biochemistry, Brandeis University, Waltham, Massachussetts 02454, USA
    Biochemistry 39:4533-42. 2000
    The conversion of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) is the committed and rate-limiting reaction in de novo guanine nucleotide biosynthesis...

Research Grants18

  1. TRACY RUTH DANIELS-WELLS; Fiscal Year: 2014
    ..Therefore, additional therapeutic strategies are urgently needed. The epithelial membrane protein-2 (EMP2) is a tetraspan membrane protein involved in cell adhesion, invasion, and signal transduction through the ..
  2. LIZBETH K HEDSTROM; Fiscal Year: 2016
    ..IMPDH catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) with the concomitant reduction of NAD+...
  3. Friedhelm Hildebrandt; Fiscal Year: 2016
    ..we identified by HM, WEC and MPS mutation of CUBN and ARHGDIA as novel single-gene causes of SRNS, and mutation of EMP2 as the first recognized cause of SSNS...
  4. Emp2: Potential Control Of Pathology In Retinal Pigment Epithelium
    Lynn Gordon; Fiscal Year: 2009
    ..Recent studies support the hypothesis that blockade of a specific protein, epithelial membrane protein 2 (EMP2), or its downstream signaling pathway, may control the biologic response of RPE cells and ..
  5. IMP DEHYDROGENASE FROM PNEUMOCYSTIS AS A DRUG TARGET
    SHERRY QUEENER; Fiscal Year: 2000
    ..carinii IMPDH, creating an insurmountable metabolic blockade because salvage pathways cannot supply adequate XMP or GMP to support proliferation of the organism. Specific aims are: 1) Purify recombinant P...
  6. Madhuri Wadehra; Fiscal Year: 2016
    ..molecular image based therapy to assess the localization and extent of breast cancer based on epithelial protein-2 (EMP2) expression...
  7. Novel Therapies to Prevent Blindness Caused by Proliferative Vitreoretinopathy
    Lynn K Gordon; Fiscal Year: 2013
    ..Recent studies support the hypothesis that control EMP2 (epithelial membrane protein 2), or its downstream signaling pathway, may change the biologic response of RPE cells and prevent or ..
  8. Targeting EMP2 in Endometrial Cancer
    Madhuri Wadehra; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The tetraspan protein EMP2 is a novel biomarker for endometrial cancer prognosis and survival...
  9. BIOSYNTHESIS AND INTERCONVERSION OF PURINES
    JOSEPH GOTS; Fiscal Year: 1980
    ..formation of the first intermediate, phosphoribosylamine, 2) the IMP-cyclohydrolase-transformylase complex, 3) IMP dehydrogenase, 4) XMP aminase, 5) GMP reductase, 6) purine phosphoribosyltransferases and 7) purine nucleoside kinases.
  10. MAPPING NUCLEOTIDE SITES OF E. COLI GMP SYNTHETASE
    OWEN MOE; Fiscal Year: 2000
    ..The ATP pyrophosphatase domain provides binding sites for the nucleotides ATP and XMP and catalyzes the formation of an adenyl-XMP intermediate that reacts with ammonia produced on the glutamine ..
  11. Abnormal EMPS Expression Affects Pregnancy Outcome
    Jonathan Braun; Fiscal Year: 2006
    ..We recently identified, on the surface of uterine epithelium, a four transmembrane protein, epithelial membrane protein 2 (EMP2) that is required for successful implantation...
  12. STRUCTURE/FUNCTION RELATIONSHIPS OF GUANYLATE KINASE
    Honggao Yan; Fiscal Year: 1999
    ..redesign the GMP site by mutagenesis so that the enzyme catalyzes phosphoryl transfer from ATP specifically to IMP, XMP, or AMP...
  13. ROLE OF THE TETRASPANIN EMP2 IN EARLY REPRODUCTION
    Carmen Williams; Fiscal Year: 2002
    ..on the surface of mouse eggs, embryos, and uterine epithelium, a novel tetraspanin protein called epithelial membrane protein 2 (EMP2)...
  14. IMP Dehydrogenase and the Hydra of Cancer Chemotherapy
    GEORGE MARKHAM; Fiscal Year: 2008
    ..the rate-limiting step in guanine nucleotide biosynthesis, the oxidation of IMP to xanthosine monophosphate (XMP)...
  15. The mechanism of signal transduction in photoreceptors
    Hartmut Luecke; Fiscal Year: 2006
    ..5. Determine the crystal structure of the primary Anabaena photoreceptor in the ground and signaling state. 6. Determine the structure of the complex of the Anabaena photoreceptor with its putative transducer. [unreadable] [unreadable]..
  16. Structure-Function Studies of Annexin I and II
    Hartmut Luecke; Fiscal Year: 2006
    ..5) Co-crystallize annexin with t-PA and solve the structure of this complex after characterizing complex formation in solution. ..
  17. STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLE
    Hartmut Luecke; Fiscal Year: 2002
    ....
  18. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2008
    ..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..