EIF4G1

Summary

Gene Symbol: EIF4G1
Description: eukaryotic translation initiation factor 4 gamma 1
Alias: EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18, eukaryotic translation initiation factor 4 gamma 1, EIF4-gamma, eIF-4-gamma 1, eucaryotic translation initiation factor 4G
Species: human

Top Publications

  1. ncbi Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation
    Thomas C Vary
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Endocrinol Metab 290:E631-42. 2006
  2. pmc HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage
    Alfredo Castello
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Nicolas Cabrera 1, Universidad Autonoma de Madrid, Cantoblanco, Spain
    PLoS ONE 4:e7997. 2009
  3. ncbi In vitro cleavage of eIF4GI but not eIF4GII by HIV-1 protease and its effects on translation in the rabbit reticulocyte lysate system
    Theophile Ohlmann
    LaboRetro, INSERM U412, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
    J Mol Biol 318:9-20. 2002
  4. ncbi Modulating HIV-1 RNA processing and utilization
    Meredith McLaren
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario
    Front Biosci 13:5693-707. 2008
  5. pmc Structure of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G: molecular mechanisms of a tumor suppressor
    Lorna C Waters
    Department of Biochemistry, Henry Wellcome Building, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 286:17270-80. 2011
  6. pmc Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E
    A Haghighat
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    EMBO J 14:5701-9. 1995
  7. pmc Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma
    Luxia Tu
    Cancer Research Institute, Key Lab for Transcriptomics and Proteomics of Human Fatal Diseases, Ministry of Education and Guangdong Province, Southern Medical University, Guangdong Province, PR China
    Mol Cancer 9:78. 2010
  8. pmc The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins
    S Mader
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 15:4990-7. 1995
  9. pmc Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A
    H Imataka
    Department of Biochemistry and McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 17:6940-7. 1997
  10. pmc A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation
    H Imataka
    Department of Biochemistry and McGill Cancer Centre, McGill University, Drummond Street 3655, Montreal, Quebec, Canada H3G 1Y6
    EMBO J 17:7480-9. 1998

Research Grants

  1. IAN J MOHR; Fiscal Year: 2016
  2. Leonidas C Platanias; Fiscal Year: 2016
  3. David A Campbell; Fiscal Year: 2014
  4. Michael D Dennis; Fiscal Year: 2014
  5. Richard E Davis; Fiscal Year: 2015
  6. Translational partitioning of the SL RNA
    David A Campbell; Fiscal Year: 2010
  7. Effects of diet and diabetes on gene expression in the liver
    Michael D Dennis; Fiscal Year: 2013
  8. Nathaniel J Moorman; Fiscal Year: 2016
  9. Carl D Novina; Fiscal Year: 2015
  10. Antifibrotic Drug Discovery in Acute Lung Injury
    Peter Bitterman; Fiscal Year: 2007

Detail Information

Publications262 found, 100 shown here

  1. ncbi Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation
    Thomas C Vary
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Am J Physiol Endocrinol Metab 290:E631-42. 2006
    ..Meal feeding enhanced the assembly of the active eIF4G.eIF4E complex, which returned to basal levels within 3 h of removal of food...
  2. pmc HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage
    Alfredo Castello
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Nicolas Cabrera 1, Universidad Autonoma de Madrid, Cantoblanco, Spain
    PLoS ONE 4:e7997. 2009
    ..This is the case of human immunodeficiency virus type 1 protease (HIV-1 PR), which hydrolyzes eIF4GI and poly(A)-binding protein (PABP)...
  3. ncbi In vitro cleavage of eIF4GI but not eIF4GII by HIV-1 protease and its effects on translation in the rabbit reticulocyte lysate system
    Theophile Ohlmann
    LaboRetro, INSERM U412, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
    J Mol Biol 318:9-20. 2002
    ..Among these proteins, eIF4GI, which is targeted by picornaviral proteases, makes a bridge between the mRNA cap structure (via eIF4E) and the 40 ..
  4. ncbi Modulating HIV-1 RNA processing and utilization
    Meredith McLaren
    Department of Molecular Genetics, University of Toronto, Toronto, Ontario
    Front Biosci 13:5693-707. 2008
    ..The observations made underline the multiple fate decisions that must be made at each stage of the viral RNA metabolic pathway and highlight potential new avenues for controlling HIV-1 replication...
  5. pmc Structure of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G: molecular mechanisms of a tumor suppressor
    Lorna C Waters
    Department of Biochemistry, Henry Wellcome Building, University of Leicester, Lancaster Road, Leicester LE1 9HN, United Kingdom
    J Biol Chem 286:17270-80. 2011
    ..is recruitment of the 43S preinitation complex to the 5' mRNA cap by the eIF4F complex (eIF4A, eIF4E, and eIF4G)...
  6. pmc Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E
    A Haghighat
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    EMBO J 14:5701-9. 1995
    ..eIF-4E forms a complex with two other polypeptides, eIF-4A and p220, that promote 40S ribosome binding to mRNA...
  7. pmc Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma
    Luxia Tu
    Cancer Research Institute, Key Lab for Transcriptomics and Proteomics of Human Fatal Diseases, Ministry of Education and Guangdong Province, Southern Medical University, Guangdong Province, PR China
    Mol Cancer 9:78. 2010
    The aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival ..
  8. pmc The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins
    S Mader
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 15:4990-7. 1995
    ..These results shed light on the mechanisms of eIF-4F assembly and on the translational regulation by insulin and growth factors...
  9. pmc Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A
    H Imataka
    Department of Biochemistry and McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 17:6940-7. 1997
    Mammalian translation initiation factor 4F (eIF4F) consists of three subunits, eIF4A, eIF4E, and eIF4G. eIF4G interacts directly with both eIF4A and eIF4E...
  10. pmc A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation
    H Imataka
    Department of Biochemistry and McGill Cancer Centre, McGill University, Drummond Street 3655, Montreal, Quebec, Canada H3G 1Y6
    EMBO J 17:7480-9. 1998
    ..Using 5' rapid amplification of cDNA, we have extended the known human eIF4GI open reading frame from the N-terminus by 156 amino acids...
  11. pmc Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E
    S Pyronnet
    Department of Biochemistry and McGill Cancer Cancer Center, McGill University, 3655 Drummond Street, Montreal, Quebec, H3G 1Y6 Canada
    EMBO J 18:270-9. 1999
    Human eukaryotic translation initiation factor 4E (eIF4E) binds to the mRNA cap structure and interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex...
  12. ncbi eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation
    A C Gingras
    Department of Biochemistry McGill University, Montreal, Quebec, Canada
    Annu Rev Biochem 68:913-63. 1999
    ..structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction with poly(A)-binding protein (eIF4G)...
  13. pmc Multiple portions of poly(A)-binding protein stimulate translation in vivo
    N K Gray
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin Madison, Madison, WI 53706, USA
    EMBO J 19:4723-33. 2000
    ..Our results establish that oocytes contain the machinery necessary to support PAB-mediated translation and suggest that PAB may be an important participant in translational regulation during early development...
  14. pmc HIV-1 protease cleaves eukaryotic initiation factor 4G and inhibits cap-dependent translation
    I Ventoso
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 98:12966-71. 2001
    ..Purified HIV-1 protease directly cleaves eIF4GI at positions 678, 681, and 1086, separating the three domains of this initiation factor...
  15. ncbi Cleavage of eIF4G by HIV-1 protease: effects on translation
    Celia Perales
    Centro de Biologi a Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autonoma CSIC, Cantoblanco 28049, Madrid, Spain
    FEBS Lett 533:89-94. 2003
    We have recently reported that HIV-1 protease (PR) cleaves the initiation factor of translation eIF4GI [Ventoso et al., Proc. Natl. Acad. Sci. USA 98 (2001) 12966-12971]...
  16. pmc Characterization of a novel RNA-binding region of eIF4GI critical for ribosomal scanning
    Déborah Prévôt
    LaboRetro, Inserm Unité de Virologie Humaine U 412, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
    EMBO J 22:1909-21. 2003
    The eukaryotic translation initiation factor eIF4GI binds several proteins and acts as a scaffold to promote preinitiation complex formation on the mRNA molecule (48S)...
  17. pmc The eukaryotic translation initiation factor 4GI is cleaved by different retroviral proteases
    Enrique Alvarez
    Centro de Biologia Molecular CSIC UAM, Facultad de Ciencias, Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain
    J Virol 77:12392-400. 2003
    ..In the present work, cleavage of both eIF4GI and eIF4GII has been analyzed by employing the proteases encoded within the genomes of several members of the ..
  18. pmc Sequestration of TRAF2 into stress granules interrupts tumor necrosis factor signaling under stress conditions
    Woo Jae Kim
    National Research Laboratory, Postech Biotech Center, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Hyoja Dong San 31, Pohang, Kyungbuk 790 784, Korea
    Mol Cell Biol 25:2450-62. 2005
    ..However, the link between these two physiological phenomena has not been clearly elucidated. Here we report that eIF4GI, which is a scaffold protein interacting with many translation factors, interacts with TRAF2, a signaling molecule ..
  19. pmc Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells
    Eileen Connolly
    Department of Microbiology, New York University School of Medicine, New York, New York 10016, USA
    Mol Cell Biol 26:3955-65. 2006
    ..Breast cancer cells therefore acquire resistance to hypoxia by uncoupling oxygen-responsive signaling pathways from mTOR function, eliminating inhibition of protein synthesis mediated by 4E-BP1 and eEF2...
  20. pmc Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit
    Aaron K LeFebvre
    Department of Biochemistry and Molecular Biology and Research Core Facility, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USA
    J Biol Chem 281:22917-32. 2006
    ..The role of mammalian eIF3 in assembly of the 48 S complex occurs through high affinity binding to eIF4G. Interactions of eIF4G with eIF4E, eIF4A, eIF3, poly(A)-binding protein, and Mnk1/2 have been mapped to discrete ..
  21. pmc p97/DAP5 is a ribosome-associated factor that facilitates protein synthesis and cell proliferation by modulating the synthesis of cell cycle proteins
    Sang Hyun Lee
    Cancer Research Institute and Comprehensive Cancer Center, University of California, San Francisco, CA 94115, USA
    EMBO J 25:4008-19. 2006
    ..In contrast, overexpression of the C-terminal two-thirds of eukaryotic initiation factor 4GI (eIF4GI), a region that shares significant homology with p97, or the N-terminal MIF4G domain of p97 markedly inhibits ..
  22. pmc Specific isoforms of translation initiation factor 4GI show differences in translational activity
    Mark J Coldwell
    Department of Biochemistry, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QG, United Kingdom
    Mol Cell Biol 26:8448-60. 2006
    The eukaryotic initiation factor (eIF) 4GI gene locus (eIF4GI) contains three identified promoters, generating alternately spliced mRNAs, yielding a total of five eIF4GI protein isoforms...
  23. pmc PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains
    Chikako Suzuki
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:3274-9. 2008
    ..We found that the PDCD4 MA3 domains compete with the eIF4G MA3 domain and RNA for eIF4A binding...
  24. pmc eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy
    Francisco Ramírez-Valle
    Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 181:293-307. 2008
    ..We show that depletion of initiation factor eIF4GI only modestly reduces overall protein synthesis in cells, but phenocopies nutrient starvation or inhibition of ..
  25. doi Lentiviral RNAs can use different mechanisms for translation initiation
    Emiliano P Ricci
    Unité de Virologie Humaine, Ecole Normale Superieure de Lyon, IFR 128, Lyon, F 69364, France
    Biochem Soc Trans 36:690-3. 2008
    ..Our results show that HIV-1 is able to drive the synthesis of the Gag polyprotein both by a classical cap-dependent mechanism and an IRES, whereas HIV-2 and SIV appear to use exclusively an IRES mechanism...
  26. pmc Requirement of RNA binding of mammalian eukaryotic translation initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E with the mRNA cap
    Akiko Yanagiya
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 29:1661-9. 2009
    ..Here, we used recombinant proteins to reconstitute the cap binding of the mammalian eIF4E-eIF4GI complex to investigate the importance of the RNA-binding region of eIF4GI for cap interaction with eIF4E...
  27. pmc Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation
    Assen Marintchev
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Cell 136:447-60. 2009
    ..Free eIF4A is a poor helicase and requires the accessory proteins eIF4G and eIF4H...
  28. doi Essential role for eIF4GI overexpression in the pathogenesis of inflammatory breast cancer
    Deborah Silvera
    Department of Microbiology, New York University School of Medicine, New York, 10016, USA
    Nat Cell Biol 11:903-8. 2009
    ..the unique pathogenic properties of IBC result in part from overexpression of the translation initiation factor eIF4GI in most IBCs...
  29. pmc A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation
    Kyoung Mi Kim
    School of Life Sciences and Biotechnology, Korea University, Seoul 136 701, Republic of Korea
    Genes Dev 23:2033-45. 2009
    ....
  30. pmc Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation
    Marc R Fabian
    Department of Biochemistry, McGill University, Montreal, QC H3G 1Y6, Canada
    Mol Cell 35:868-80. 2009
    ..Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation...
  31. pmc Translation initiator EIF4G1 mutations in familial Parkinson disease
    Marie Christine Chartier-Harlin
    Universite Lille Nord de France
    Am J Hum Genet 89:398-406. 2011
    ..3614G>A (p.Arg1205His) in eukaryotic translation initiation factor 4-gamma (EIF4G1). Subsequent sequence and genotype analysis identified EIF4G1 c.1505C>T (p.Ala502Val), c.2056G>T (p...
  32. doi Variants in eukaryotic translation initiation factor 4G1 in sporadic Parkinson's disease
    Eva C Schulte
    Neurologische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universitat Munchen, Ismaningerstr 22, 81675 Munich, Germany
    Neurogenetics 13:281-5. 2012
    Recently, mutations in eukaryotic translation initiation factor 4G1 (EIF4G1) were reported as a rare cause of familial Parkinson's disease (PD)...
  33. pmc DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs
    Ricardo Soto-Rifo
    Human Virology Department, INSERM U, Lyon, France
    EMBO J 31:3745-56. 2012
    ..by the DEAD-box helicase DDX3 which can directly bind to the 5' of the target mRNA where it clamps the entry of eIF4F through an eIF4G and Poly A-binding protein cytoplasmic 1 (PABP) double interaction...
  34. pmc A novel functional human eukaryotic translation initiation factor 4G
    A Gradi
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada
    Mol Cell Biol 18:334-42. 1998
    ..To date, however, only one functional eIF4G polypeptide, referred to here as eIF4GI, has been identified in mammals...
  35. ncbi Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo
    W Li
    Division of Molecular Physiology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
    J Biol Chem 276:29111-5. 2001
    ..These include eIF4G, a modular scaffolding protein, and eIF4A, an RNA helicase, of which two closely related forms are known in ..
  36. pmc Generation of multiple isoforms of eukaryotic translation initiation factor 4GI by use of alternate translation initiation codons
    Marshall P Byrd
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 22:4499-511. 2002
    Eukaryotic translation initiation factor 4GI (eIF4GI) is an essential protein that is the target for translational regulation in many cellular processes and viral systems...
  37. ncbi Up-regulation of PDCD4 in senescent human diploid fibroblasts
    Min Ji Kang
    Department of Biochemistry and Molecular Biology and Ilchun Molecular Medicine Institute, Seoul National University College of Medicine, 28 Yongondong, Chongnogu, Seoul 110 799, Republic of Korea
    Biochem Biophys Res Commun 293:617-21. 2002
    ..domain sharing with death associated protein 5 (DAP5) and a component of eukaryotic translation initiation factor (eIF4G) complex and it might also work as a tumor suppressor...
  38. ncbi Frequent overexpression of the genes FXR1, CLAPM1 and EIF4G located on amplicon 3q26-27 in squamous cell carcinoma of the lung
    Nicole Comtesse
    Department of Human Genetics, Medical School, Saarland University, Homburg, Germany
    Int J Cancer 120:2538-44. 2007
    ..Three genes, namely FXR1, CLAPM1 and EIF4G, are most frequently overexpressed in the center of the amplified domain in squamous cell carcinomas...
  39. pmc Study of the genetic variability in a Parkinson's Disease gene: EIF4G1
    Arianna Tucci
    Department of Molecular Neuroscience and Reta Lila Weston Laboratories, Institute of Neurology, Queen Square House, Queen Square, London WC1N 3BG, United Kingdom
    Neurosci Lett 518:19-22. 2012
    ..and colleagues [2] recently reported mutations in the eukaryotic translation initiation factor 4-gamma (EIF4G1) gene in families with parkinsonism. Large-scale screening found two mutations (p.R1205H and p...
  40. ncbi Regulation of the rapamycin and FKBP-target 1/mammalian target of rapamycin and cap-dependent initiation of translation by the c-Abl protein-tyrosine kinase
    V Kumar
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 275:10779-87. 2000
    ..These findings with the c-Abl tyrosine kinase represent the first demonstration of a negative physiologic regulator of RAFT1-mediated 5' cap-dependent translation...
  41. pmc Hybrid proteins between Pseudomonas aeruginosa exotoxin A and poliovirus 2Apro cleave p220 in HeLa cells
    I Novoa
    , , , Spain
    J Virol 70:3319-24. 1996
    Cleavage of p220, a component of the initiation factor eIF-4F, has been correlated with the inhibition of host translation during poliovirus infection...
  42. ncbi Acute oral leucine administration stimulates protein synthesis during chronic sepsis through enhanced association of eukaryotic initiation factor 4G with eukaryotic initiation factor 4E in rats
    Thomas C Vary
    Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey, PA 17033, USA
    J Nutr 137:2074-9. 2007
    ..Increased assembly of the active eIF4G-eIF4E complex was associated with a robust rise in phosphorylation of eIF4G(Ser(1108)) and a decreased assembly of ..
  43. doi Nitric oxide mediates NMDA-induced persistent inhibition of protein synthesis through dephosphorylation of eukaryotic initiation factor 4E-binding protein 1 and eukaryotic initiation factor 4G proteolysis
    Valerie Petegnief
    Departamento de Isquemia Cerebral y Neurodegeneración, Instituto de Investigaciones Biomédicas de Barcelona, CSIC IDIBAPS, Rossello 161, Barcelona 08036, Spain
    Biochem J 411:667-77. 2008
    ..In conclusion, our data show that NO mediates NMDA-induced persistent translation inhibition and suggest that deficient eIF4F activity contributes to this process.
  44. ncbi Age-associated decrease in contraction-induced activation of downstream targets of Akt/mTor signaling in skeletal muscle
    Katsuhiko Funai
    Nutrition, Exercise Physiology, and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, 711 Washington St, Boston, MA 02111, USA
    Am J Physiol Regul Integr Comp Physiol 290:R1080-6. 2006
    ..effect of age on the association of eukaryotic initiation factor 4E (eIF4E) with eukaryotic initiation factor 4G (eIF4G), as well as the activity of its binding protein (4E-BP1) and the activity of glycogen synthase kinase-3 (GSK-3) ..
  45. pmc Oral leucine enhances myocardial protein synthesis in rats acutely administered ethanol
    Thomasc Vary
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, PA 17033, USA
    J Nutr 139:1439-44. 2009
    ..eIF4E complex. Increased assembly of the active eIF4G.eIF4E complex was associated with a 130% rise in phosphorylation of eIF4G(Ser(1108)) and a decreased assembly ( ..
  46. pmc A new translational regulator with homology to eukaryotic translation initiation factor 4G
    H Imataka
    Department of Biochemistry and McGill Cancer Centre, McGill University, Montreal, Quebec, Canada
    EMBO J 16:817-25. 1997
    ..Eukaryotic translation initiation factor 4F (eIF4F) is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G...
  47. pmc DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death
    N Levy-Strumpf
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Mol Cell Biol 17:1615-25. 1997
    ..that it codes for a 97-kDa protein that is highly homologous to eukaryotic translation initiation factor 4G (eIF4G, also known as p220)...
  48. ncbi Identification of caspase 3-mediated cleavage and functional alteration of eukaryotic initiation factor 2alpha in apoptosis
    W E Marissen
    Department of Microbiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA
    J Biol Chem 275:9314-23. 2000
    ..Recently, the cleavage of eukaryotic translation initiation factor 4G (eIF4G) by caspase 3 was described as a possible event contributing to translation inhibition...
  49. pmc Detailed analysis of the requirements of hepatitis A virus internal ribosome entry segment for the eukaryotic initiation factor complex eIF4F
    A M Borman
    Unité Postulante de Régulation de la Traduction Eucaryote et Virale, CNRS URA 1966, Institut Pasteur, 75724 Paris Cedex 15, France
    J Virol 75:7864-71. 2001
    ..Since these proteinases both cleave eukaryotic initiation factor 4G (eIF4G) and HAV IRES activity could be rescued in vitro by addition of eIF4F to proteinase-treated extracts, it was ..
  50. ncbi Cytokine-triggered decreases in levels of phosphorylated eukaryotic initiation factor 4G in skeletal muscle during sepsis
    Thomas C Vary
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Shock 26:631-6. 2006
    ..The extent of phosphorylated eIF4G was reduced by more than 50% (P< 0.05) and 68% (P < 0...
  51. pmc The eukaryotic initiation factor (eIF) 4G HEAT domain promotes translation re-initiation in yeast both dependent on and independent of eIF4A mRNA helicase
    Ryosuke Watanabe
    Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 285:21922-33. 2010
    ..eIF4G is the major adaptor subunit of eIF4F that binds the cap-binding subunit eIF4E and the mRNA helicase eIF4A and is also required for re-initiation in ..
  52. ncbi Interference with interaction between eukaryotic translation initiation factor 4G and poly(A)-binding protein in Xenopus oocytes leads to inhibition of polyadenylated mRNA translation and oocyte maturation
    M Wakiyama
    Institute for Biomolecular Science, Gakushuin University, 1 5 1 Mejiro, Toshima ku, Tokyo 171 8588, Japan
    J Biochem 130:737-40. 2001
    ..It was shown recently that the expression of an eIF4GI mutant defective in PABP binding in Xenopus oocytes reduces polyadenylated mRNA translation and dramatically ..
  53. ncbi Chronic alcohol feeding impairs mTOR(Ser 2448) phosphorylation in rat hearts
    Thomas C Vary
    Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Alcohol Clin Exp Res 32:43-51. 2008
    ....
  54. pmc Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E
    Yikun Li
    Department of Hematology and Medical Oncology, Emory University School of Medicine and Winship Cancer Institute, Atlanta, GA 30322, USA
    Neoplasia 12:848-55. 2010
    ..in PP2A siRNA-transfected cells than in control siRNA-transfected cells, indicating an increased cap binding of eIF4F complex...
  55. ncbi Immunohistochemical mapping of total and phosphorylated eukaryotic initiation factor 4G in rat hippocampus following global brain ischemia and reperfusion
    D J DeGracia
    Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA
    Neuroscience 139:1235-48. 2006
    Partial proteolysis and phosphorylation of the translation initiation factor eukaryotic initiation factor 4G (eIF4G) occur in reperfused brain, but the contribution of eIF4G alterations to brain injury has not been established...
  56. doi Competitive and noncompetitive binding of eIF4B, eIF4A, and the poly(A) binding protein to wheat translation initiation factor eIFiso4G
    Shijun Cheng
    Department of Biochemistry, University of California, Riverside, California 92521 0129, USA
    Biochemistry 49:8251-65. 2010
    Eukaryotic translation initiation factor 4G (eIF4G) functions to organize the assembly of initiation factors required for the recruitment of a 40S ribosomal subunit to an mRNA and for interacting with the poly(A) binding protein (PABP)...
  57. pmc A novel function of the MA-3 domains in transformation and translation suppressor Pdcd4 is essential for its binding to eukaryotic translation initiation factor 4A
    Hsin Sheng Yang
    Gene Regulation Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA
    Mol Cell Biol 24:3894-906. 2004
    ..appears in several translation initiation factors, including human eukaryotic translation initiation factor 4G (eIF4G) and DAP-5/NAT1/p97, as well as in the tumor suppressor Pdcd4...
  58. ncbi Mapping of functional domains in eukaryotic protein synthesis initiation factor 4G (eIF4G) with picornaviral proteases. Implications for cap-dependent and cap-independent translational initiation
    B J Lamphear
    Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130 3932, USA
    J Biol Chem 270:21975-83. 1995
    ..S ribosomal subunit during translational initiation requires the association of eukaryotic initiation factor 4G (eIF4G; formerly eIF-4 gamma and p220) with other initiation factors, notably eIF4E, eIF4A, and eIF3...
  59. ncbi Intact eukaryotic initiation factor 4G is required for hepatitis A virus internal initiation of translation
    A M Borman
    CNRS URA 1966, Institut Pasteur, Paris, France
    Virology 237:129-36. 1997
    ..common cellular target for the 2A and Lb proteinases, eukaryotic initiation factor (eIF) 4G, a component of the eIF4F cap-binding protein complex...
  60. pmc The C terminus of initiation factor 4E-binding protein 1 contains multiple regulatory features that influence its function and phosphorylation
    Xuemin Wang
    Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 23:1546-57. 2003
    Eukaryotic initiation factor 4E (eIF4E) binds the mRNA cap structure and forms eIF4F complexes that recruit 40S subunits to the mRNA...
  61. pmc High affinity RNA for mammalian initiation factor 4E interferes with mRNA-cap binding and inhibits translation
    Kiyotaka Mochizuki
    Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    RNA 11:77-89. 2005
    The eukaryotic translation initiation factor 4F (eIF4F) consists of three polypeptides (eIF4A, eIF4G, and eIF4E) and is responsible for recruiting ribosomes to mRNA...
  62. ncbi Activation of p53 stimulates proteasome-dependent truncation of eIF4E-binding protein 1 (4E-BP1)
    Constantina Constantinou
    Translational Control Group, Centre for Molecular and Metabolic Signalling, Division of Basic Medical Sciences, St George s, University of London, Cranmer Terrace, London SW17 0RE, U K
    Biol Cell 100:279-89. 2008
    ..Its ability to associate with initiation factors eIF4G and eIF4A, forming the eIF4F complex, brings the mRNA to the 43S complex during the initiation of translation...
  63. doi Blocking eukaryotic initiation factor 4F complex formation does not inhibit the mTORC1-dependent activation of protein synthesis in cardiomyocytes
    Brandon P H Huang
    Department of Biochemistry and Molecular Biology, University of British Columbia and Diabetes Research Group, Life Sciences Institute, Vancouver, Canada
    Am J Physiol Heart Circ Physiol 296:H505-14. 2009
    ..of eukaryotic initiation factor 4E complex (eIF4E)-binding protein 1 (4E-BP1) from eIF4E, leading to increased eIF4F complex formation...
  64. ncbi Rapamycin limits formation of active eukaryotic initiation factor 4F complex following meal feeding in rat hearts
    Thomas C Vary
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    J Nutr 137:1857-62. 2007
    ..herein examined the potential role for a rapamycin-sensitive signaling pathway in increasing formation of active eIF4G-eIF4E complex during meal feeding...
  65. ncbi Eukaryotic translation initiation factor 4E induced progression of primary human mammary epithelial cells along the cancer pathway is associated with targeted translational deregulation of oncogenic drivers and inhibitors
    Ola Larsson
    Department of Medicine, University of Minnesota, 420 Delaware Street, Minneapolis, MN 55455, USA
    Cancer Res 67:6814-24. 2007
    ..redirection of translational control by constitutive activation of eukaryotic translation initiation factor 4F (eIF4F), the cap-dependent translation initiation apparatus, is an obligatory step in oncogenesis; however, its mechanism ..
  66. doi Effects of poly(A)-binding protein on the interactions of translation initiation factor eIF4F and eIF4F.4B with internal ribosome entry site (IRES) of tobacco etch virus RNA
    Mateen A Khan
    Department of Chemistry, Hunter College and the Graduate Center of the City University of New York, New York, NY 10065, USA
    Biochim Biophys Acta 1779:622-7. 2008
    ..etch virus to promote cap-independent translation led us to investigate the effects of PABP on the interaction of eIF4F with PK1...
  67. ncbi eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap-independent translation, and translation of structured mRNAs
    D R Gallie
    Department of Biochemistry, University of California, Riverside, California 92521 0129, USA
    J Biol Chem 276:36951-60. 2001
    ..differences between plant eIF4G proteins, referred to as eIF4G and eIFiso4G, when present as subunits of eIF4F and eIFiso4F, respectively...
  68. pmc Carbon- and nitrogen-quality signaling to translation are mediated by distinct GATA-type transcription factors
    F G Kuruvilla
    Department of Chemistry and Chemical Biology, Howard Hughes Medical Institute, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 98:7283-8. 2001
    ..In their absence, processes caused by rapamycin or poor nutrients are diminished: translation repression, eIF4G protein loss, transcriptional down-regulation of proteins involved in translation, and RNA polymerase I/III ..
  69. pmc Paip1 interacts with poly(A) binding protein through two independent binding motifs
    Guylaine Roy
    Department of Biochemistry and McGill Cancer Centre, McGill University, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 22:3769-82. 2002
    ..The poly(A) binding protein (PABP) interacts with eukaryotic initiation factor 4G (eIF4G), a component of the eIF4F complex, which binds to the 5' cap structure...
  70. pmc Evidence for variation in the optimal translation initiation complex: plant eIF4B, eIF4F, and eIF(iso)4F differentially promote translation of mRNAs
    Laura K Mayberry
    Department of Chemistry and Biochemistry and the Institute for Cell and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    Plant Physiol 150:1844-54. 2009
    ..would facilitate the multiple interactions of eIF4B with mRNA as well as other initiation factors (eIF4A, eIF4G, PABP, and eIF3)...
  71. ncbi Thermal injury impairs cardiac protein synthesis and is associated with alterations in translation initiation
    Charles H Lang
    Department of Cellular and Molecular Physiology H166, Penn State College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Regul Integr Comp Physiol 286:R740-50. 2004
    ..increased binding of the translational repressor 4E-BP1 with eIF4E, 2) a decreased amount of eIF4E associated with eIF4G, and 3) a decreased amount of the hyperphosphorylated gamma-form of 4E-BP1...
  72. ncbi Cleavage of eukaryotic translation initiation factor 4GII correlates with translation inhibition during apoptosis
    W E Marissen
    Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, OK 73104, USA
    Cell Death Differ 7:1234-43. 2000
    Eukaryotic translation initiation factor 4G (eIF4G), which has two homologs known as eIF4GI and eIF4GII, functions in a complex (eIF4F) which binds to the 5' cap structure of cellular mRNAs and facilitates binding of capped mRNA to 40S ..
  73. ncbi A tale of two termini: a functional interaction between the termini of an mRNA is a prerequisite for efficient translation initiation
    D R Gallie
    Department of Biochemistry, University of California, Riverside, CA 92521 0129, USA
    Gene 216:1-11. 1998
    ..of which is to increase the affinity of PABP for poly(A) and to increase the affinity of the cap-binding complex, eIF4F (of which eIF4G is a subunit) for the 5' cap structure...
  74. pmc The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs
    Adam Wallace
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Mol Cell Biol 30:1958-70. 2010
    Eukaryotic mRNA translation begins with recruitment of the 40S ribosome complex to the mRNA 5' end through the eIF4F initiation complex binding to the 5' m(7)G-mRNA cap...
  75. pmc Translation initiation factor 4B homodimerization, RNA binding, and interaction with Poly(A)-binding protein are enhanced by zinc
    Shijun Cheng
    Department of Biochemistry, University of California, Riverside, California 92521 0129, USA
    J Biol Chem 283:36140-53. 2008
    ..eIF) 4B promotes the RNA-dependent ATP hydrolysis activity and ATP-dependent RNA helicase activity of eIF4A and eIF4F during translation initiation...
  76. pmc Regulation of host translational machinery by African swine fever virus
    Alfredo Castello
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS Pathog 5:e1000562. 2009
    ..Phosphorylation of eIF4E, eIF4GI and 4E-BP1 is important to enhance viral protein production, but is not essential for ASFV infection as observed ..
  77. doi Enhance tumor radiosensitivity by intracellular delivery of eukaryotic translation initiation factor 4E binding proteins
    Shuang Tian
    Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, No 17 Changle Western Road, Xi an 710032, China
    Med Hypotheses 76:246-8. 2011
    ..in a broad array of human malignancies, controls the assembly of eukaryotic translation initiation factor 4F (eIF4F) complex through regulation of eIF4E binding proteins (4E-BPs) phosphorylation...
  78. pmc Activity of the hepatitis A virus IRES requires association between the cap-binding translation initiation factor (eIF4E) and eIF4G
    I K Ali
    Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom
    J Virol 75:7854-63. 2001
    The question of whether translation initiation factor eIF4E and the complete eIF4G polypeptide are required for initiation dependent on the IRES (internal ribosome entry site) of hepatitis A virus (HAV) has been examined using in vitro ..
  79. ncbi Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and eIFiso4F
    Mateen A Khan
    Department of Chemistry, Hunter College and the Graduate Center of the City University of New York, New York, NY 10065, USA
    J Biol Chem 283:1340-9. 2008
    ..To understand the molecular basis of these effects, we have characterized the interaction of VPg with eIF4F, eIFiso4F, and a structured RNA derived from tobacco etch virus (TEV RNA)...
  80. doi Differential contribution of the MTOR and MNK pathways to the regulation of mRNA translation in meiotic and postmeiotic mouse male germ cells
    Valeria Messina
    Section of Anatomy, Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy
    Biol Reprod 83:607-15. 2010
    ..All translation factors analyzed were expressed in germ cells throughout spermatogenesis. However, while EIF4G and PABP1 (poly[A]-binding protein 1) were more abundant in postmeiotic cells, MTOR and its target EIF4EBP1 (4E-..
  81. pmc Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sites
    Sylvain de Breyne
    Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
    Proc Natl Acad Sci U S A 106:9197-202. 2009
    ..V of type 1 IRESs, which is adjacent to the Yn-Xm-AUG motif, specifically interacts with the central domain of eIF4G. The position and orientation of eIF4G relative to the Yn-Xm-AUG motif is analogous in type 1 and 2 IRESs...
  82. ncbi IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis
    Thomas C Vary
    Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey, PA 17033, USA
    Am J Physiol Regul Integr Comp Physiol 290:R313-21. 2006
    ..4E-BP1 complex. Assembly of the active eIF4F complex (as assessed by the association eIF4G with eIF4E) was increased twofold by IGF-I in the perfusate...
  83. pmc A novel mechanism for Bcr-Abl action: Bcr-Abl-mediated induction of the eIF4F translation initiation complex and mRNA translation
    S Prabhu
    The Division of Hematology Oncology, University of California, Irvine, CA 92697, USA
    Oncogene 26:1188-200. 2007
    ..and primary CML cells indicated that Bcr-Abl and mTORC1 induced formation of the translation initiation complex, eIF4F. This was characterized by reduced 4E-BP1 binding and increased eIF4G binding to eIF4E, two events that lead to ..
  84. doi Therapeutic inhibition of MAP kinase interacting kinase blocks eukaryotic initiation factor 4E phosphorylation and suppresses outgrowth of experimental lung metastases
    Bruce W Konicek
    Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    Cancer Res 71:1849-57. 2011
    ..This liberates eIF4E and allows binding to eIF4G. eIF4E can then be phosphorylated at serine 209 by the MAPK-interacting kinases (Mnk), which also interact with ..
  85. doi Inhibition of ovarian cancer growth by a tumor-targeting peptide that binds eukaryotic translation initiation factor 4E
    Song Yi Ko
    Department of Systems Biology, University of Texas M D Anderson Cancer Center, and Cancer Biology Program, Graduate School of Biomedical Sciences, University of Texas Health Sciences Center, Houston, Texas, USA
    Clin Cancer Res 15:4336-47. 2009
    ..initiation factor eIF4E from 4EBP inhibitory binding proteins, and its engagement to the scaffolding protein eIF4G. eIF4E is a candidate target for cancer therapy because it is overexpressed or activated in many types of tumors ..
  86. pmc Novel translational control in Arc-dependent long term potentiation consolidation in vivo
    Debabrata Panja
    Department of Biomedicine and Bergen Mental Health Research Center, University of Bergen, Jonas Lies vei 91, 5009 Bergen, Norway
    J Biol Chem 284:31498-511. 2009
    ..expression, blocked changes in eIF4E and eIF2alpha phosphorylation state, and prevented initiation complex (eIF4F) formation...
  87. ncbi Characterization of the two eIF4A-binding sites on human eIF4G-1
    N L Korneeva
    Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport 71130 3932, USA
    J Biol Chem 276:2872-9. 2001
    Eukaryotic translation initiation factor 4G-1 (eIF4G) plays a critical role in the recruitment of mRNA to the 43 S preinitiation complex...
  88. pmc Control of eIF4E cellular localization by eIF4E-binding proteins, 4E-BPs
    Liwei Rong
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
    RNA 14:1318-27. 2008
    Eukaryotic initiation factor (eIF) 4E, the mRNA 5'-cap-binding protein, mediates the association of eIF4F with the mRNA 5'-cap structure to stimulate cap-dependent translation initiation in the cytoplasm...
  89. pmc Linking the 3' poly(A) tail to the subunit joining step of translation initiation: relations of Pab1p, eukaryotic translation initiation factor 5b (Fun12p), and Ski2p-Slh1p
    A Searfoss
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0830, USA
    Mol Cell Biol 21:4900-8. 2001
    ..be due to an interaction of the poly(A) binding protein, Pab1p, with eukaryotic translation initiation factor 4G (eIF4G)...
  90. ncbi Cleavage of translation initiation factor 4G (eIF4G) during anti-Fas IgM-induced apoptosis does not require signalling through the p38 mitogen-activated protein (MAP) kinase
    S J Morley
    Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK
    FEBS Lett 438:41-8. 1998
    ..In this study, we show that activation of the Fas/CD95 receptor complex in Jurkat cells induces the degradation of eIF4G, the inhibition of total protein synthesis and cell death...
  91. pmc Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism
    A C Gingras
    Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, H3G 1Y6, Canada
    Genes Dev 13:1422-37. 1999
    ..The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure...
  92. doi Regulation of cap-dependent translation initiation in the early stage porcine parthenotes
    Andrej Susor
    Institute of Animal Physiology and Genetics, Libechov, Czech Republic
    Mol Reprod Dev 75:1716-25. 2008
    ..EIF4F is a three subunit complex consisting of eIF4A (RNA helicase), eIF4E (mRNA cap binding protein), and eIF4G (bridging protein)...
  93. doi Analysis of mRNA associated factors during bovine oocyte maturation and early embryonic development
    Corinna Siemer
    Research Institute for the Biology of Farm Animals FBN Dummerstorf, 18196 Dummerstorf, Germany
    Mol Reprod Dev 76:1208-19. 2009
    ..model of translational initiation, we have analyzed components of the 5 -mRNA cap-binding complex eIF4F (eIF4E, eIF4G, eIF4A), the eIF4E repressor 4E-BP1, and 3 -mRNA poly-(A) tail-associated proteins (PABP1 and 3, PAIP1 and 2, ..
  94. pmc TIF4631 and TIF4632: two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor 4F) contain an RNA recognition motif-like sequence and carry out an essential function
    C Goyer
    Department of Biochemistry, McGill University, Montreal, Quøebec, Canada
    Mol Cell Biol 13:4860-74. 1993
    ..p150 is presumed to be the yeast homolog of the p220 component of mammalian eIF-4F...
  95. ncbi Characterization of wild-type and Ser53 mutant eukaryotic initiation factor 4E overexpression in mammalian cells
    R J Kaufman
    Genetics Institute, Cambridge, Massachusetts 02140
    J Biol Chem 268:11902-9. 1993
    ..These results demonstrate that phosphorylation of eIF-4E at residue 53 is not required for interaction with p220 and suggest that Ser53 phosphorylation may not be required for cap-dependent translation initiation in this system.
  96. pmc Structure of the foot-and-mouth disease virus leader protease: a papain-like fold adapted for self-processing and eIF4G recognition
    A Guarné
    Centre d Investigació i Desenvolupament CSIC, Jordi Girona Salgado 18 26, E 08034 Barcelona, Spain
    EMBO J 17:7469-79. 1998
    ..host cell protein synthesis by specific proteolysis of a cellular protein: the eukaryotic initiation factor 4G (eIF4G)...
  97. pmc Poliovirus 2A protease induces apoptotic cell death
    D Goldstaub
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 20:1271-7. 2000
    ..Upon induction, cleavage of the eukaryotic translation initiation factor 4GI (eIF4GI) and eIF4GII is observed, with the latter being cleaved in a somewhat slower kinetics...
  98. pmc Glucose and amino acids modulate translation factor activation by growth factors in PC12 cells
    M Kleijn
    Department of Anatomy and Physiology, MSI Wellcome Trust Building, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, UK
    Biochem J 347:399-406. 2000
    ..In nutrient-deprived cells, an increase in the phosphorylation of eIF4E, and the assembly of the eIF4F complex by EGF and NGF, coincided with the activation of protein synthesis...
  99. pmc Cell cycle-regulated phosphorylation of p220(NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription
    T Ma
    Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Genes Dev 14:2298-313. 2000
    ..E/Cdk2 controls S-phase events, we examined the subcellular localization of the cyclin E/Cdk2 interacting protein p220(NPAT) and its regulation by phosphorylation. p220 is localized to discrete nuclear foci...
  100. pmc Possible mechanisms involved in the down-regulation of translation during transient global ischaemia in the rat brain
    C Martín De La Vega
    Departamento de Investigacion, Hospital Ramon y Cajal, Ctra Colmenar Km 9, 28034 Madrid, Spain
    Biochem J 357:819-26. 2001
    ..and it was lower in the CA1-subfield than in the RH, roughly paralleling the observed decrease in eIF4E and eIF4G levels...
  101. pmc Drosophila Cup is an eIF4E-binding protein that functions in Smaug-mediated translational repression
    Meryl R Nelson
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 23:150-9. 2004
    ..that Smaug interacts with the Cup protein and that Cup is an eIF4E-binding protein that blocks the binding of eIF4G to eIF4E. Cup mediates an indirect interaction between Smaug and eIF4E, and Smaug function in vivo requires Cup...

Research Grants71

  1. IAN J MOHR; Fiscal Year: 2016
    ..We specifically propose to i) determine how HCMV infection affects eIF4F-core and associated components;ii) define the mechanism(s) by which PABP abundance is controlled translationally ..
  2. Leonidas C Platanias; Fiscal Year: 2016
    ..and regulates cap-dependent mRNA translation via control of the eukaryotic initiation factor 4E (eIF4E) and the eIF4F complex...
  3. David A Campbell; Fiscal Year: 2014
    ..the role and function of TbeIF4E5, which binds to three variants of the trypanosome translation initiation factor eIF4G and is otherwise uncharacterized...
  4. Michael D Dennis; Fiscal Year: 2014
    ..pathway and O-GlcNAcylation of key components of the mRNA cap-binding complex, including eIF4E binding protein 1, eIF4G, eIF4A, and poly(A)-binding protein, under conditions of diabetes-induced hyperglycemia...
  5. Richard E Davis; Fiscal Year: 2015
    ..the "SL effect");3) Efficient translation of the m2,2,7G-SL mRNAs requires adaptations in Ascaris eIF4E-3 and eIF4G translation initiation proteins;4) We determined the crystal structures of Ascaris eIF4E-3 bound to the two ..
  6. Translational partitioning of the SL RNA
    David A Campbell; Fiscal Year: 2010
    ..is a new addition to our hypothesis on the maturation of SL RNA involving a 'mask'protein that prevents eIF4F translation-initiation complex formation on the unspliced substrate...
  7. Effects of diet and diabetes on gene expression in the liver
    Michael D Dennis; Fiscal Year: 2013
    ..inhibit cap-dependent translation by binding to the cap-binding protein eIF4E and preventing its association with eIF4G. 5'-cap binding by eIF4E is typically thought of as the rate-limiting step in translation initiation, and as ..
  8. Nathaniel J Moorman; Fiscal Year: 2016
    ..Previous studies have shown that HCMV induces the expression and activity of the eIF4F translation initiation complex, which is required for the translation of capped mRNAs...
  9. Carl D Novina; Fiscal Year: 2015
    ..Third, eIF4E has been implicated in miRNA-mediated repression and robust increases in eIF4F (a complex of eIF4E, eIF4A and eIF4G) inhibit miRNA function...
  10. Antifibrotic Drug Discovery in Acute Lung Injury
    Peter Bitterman; Fiscal Year: 2007
    ..by the level and activity of the mRNA cap-binding apparatus, eukaryotic translation initiation factor 4F (eIF4F)...
  11. Christopher S Fraser; Fiscal Year: 2016
    ..to the 40S ribosomal subunit, promoting mRNA recruitment through an insulin-regulated interaction with the eIF4G subunit of the cap-binding complex (eIF4E, eIF4G and eIF4A)...
  12. WYATT ALLEN MILLER; Fiscal Year: 2016
    ..i) the Barley yellow dwarf virus-like translation element (BTE) which binds and requires initiation factor eIF4G and not eIF4E;and (ii) the Panicum mosaic virus-like translation element (PTE), which binds and requires eIF4E - a ..
  13. mTOR Signaling Pathways
    Thomas W Sturgill; Fiscal Year: 2010
    ..factor, we discovered a dramatic rapamycin-sensitive action of insulin to stimulate the association of eIF3 and eIF4G. Aim 1 is to investigate this novel and potentially important effect of insulin...
  14. X-RAY STRUCTURES OF EUKARYOTIC TRANSLATION FACTORS
    STEPHEN BURLEY; Fiscal Year: 2003
    ..the structures and mechanisms of action of an important subset of the remaining translation initiation factors [eIF4G, eIF4A, eIF2, IF2, eIF3, eIF1A, eIF2B, eIF5, the poly (A)-binding protein (PABP), and a PABP-interacting protein]...
  15. Assays for Discovery of Translation Inhibitors
    Gerhard Wagner; Fiscal Year: 2009
    ..The main mechanism used by most cellular mRNAs, named cap dependent translation, requires cap binding complex eIF4F. The eIF4F complex composed of the cap binding subunit eIF4E, the RNA helicase eIF4A, and the scaffolding protein ..
  16. Yingjie Chen; Fiscal Year: 2014
    ..eIF4E is the central component of the cap-dependent translation initiation complex (eIF4F)...
  17. Tatyana V Pestova; Fiscal Year: 2016
    ..In Aim 1, we will investigate the mechanistic aspects of entry of eIF4F-bound capped mRNAs into the mRNA-binding cleft of the 40S subunit during attachment of 43S complexes by ..
  18. Development of Translation Initiation Inhibitors and their Anti-Cancer Activity
    Michael Chorev; Fiscal Year: 2012
    ..throughput screening of chemical libraries inhibits eIF4E/eIF4G interaction that is essential for the formation of eIF4F complex (a critical regulatory step in the translation initiation cascade) is an inhibitor of cap-dependent ..
  19. Translational Apparatus as a Target for Drug Discovery
    Vitaly Polunovsky; Fiscal Year: 2004
    ..We have recently discovered that inhibiting assembly of the cap-dependent translation initiation apparatus eIF4F, (a trimolecular complex of eIF4G, eIF4A and eIF4E) in Ras Vl2 transformed cells by transfer of the gene encoding ..
  20. Control of elF4F complex by Vesicular Stomatitis Virus
    John Connor; Fiscal Year: 2007
    ..strand RNA virus, vesicular stomatitis virus (VSV), interacts with the translation initiation factor complex eIF4F. eIF4F is a multi-protein complex, and its function is know to be the target of many viruses such as poliovirus, ..
  21. Joan A Steitz; Fiscal Year: 2014
    ..The probable existence of additional eIF4A/eIF4G-like pairs will be explored and their contributions to critical steps in gene expression assigned...
  22. Molecular determinants of host-cell interactions required for arenavirus replicat
    Jack H Nunberg; Fiscal Year: 2013
    ..We posit that N acts as a surrogate to bind m7G-capped viral mRNA and recruit the eIF4G scaffold protein, in order to gain privileged access to ribosomes...
  23. REGULATION OF RETROTRANSPOSITION IN S. CEREVISIAE
    M Joan Curcio; Fiscal Year: 2013
    ..We have recently found that eIF4G1 and two ribosomal protein paralogs are required for accumulation of TyA, the major capsid protein, while Xrn1 ..
  24. Amino Acids and Muscle Protein Synthesis
    Robert Wolfe; Fiscal Year: 2006
    ..an mTOR-independent (ie, rapamycin- insensitive) signaling pathway resulting in an increased phosphorylation of eIF4G. We will investigate a series of specific hypotheses related to these general proposals using a porcine model that ..
  25. Dissecting Translational Regulation by Genome-Wide Mapping of Initiation Factors
    OLIVIA SELFRIDGE RISSLAND; Fiscal Year: 2013
    ..To test my central hypothesis that the miRNA-mediated translational repression acts by disrupting eIF4G binding, I will map eIF4G-mRNA interactions transcriptome-wide and determine how these interactions are affected ..
  26. Clyde L Denis; Fiscal Year: 2016
    ..The 21S/28S complexes (about 0.5 to 1.4 MDa depending on their shape) contained at least eRF1, eIF4E, eIF4G1, PAB1, HRP1, and mRNA...
  27. Translational Control in IPF
    Peter B Bitterman; Fiscal Year: 2011
    ..that autonomy is conferred by deregulation of the cap-dependent translation initiation machinery, designated eIF4F. In normal cells, eIF4F receives signals from matrix and growth factor receptors and their downstream ..
  28. CONTROL OF TRANSLATION BY POLIOVIRUS PROTEINASE
    Richard Lloyd; Fiscal Year: 1999
    ..Further, it is believed that inactivation of CBP complex results from cleavage of the p220 component of the complex...
  29. Gerhard Wagner; Fiscal Year: 2015
    ..mechanisms by which the small ribosomal particle is recruited to mRNA, mediated by the initiation factors eIF4E, eIF4G and the regulatory phosphoprotein 4EBP-1...
  30. IRES-mediated translation initiation on viral mRNAs
    CHRISTOPHER ULRICH TRISTRAM HELLEN; Fiscal Year: 2013
    ..Type 2 picornavirus IRESs such as the encephalomyocarditis virus IRES bind specifically to eIF4G/eIF4A: we will investigate how these factors promote recruitment of 43S complexes to the initiation codon, how ..
  31. Scot R Kimball; Fiscal Year: 2016
    ..and specific changes in protein expression patterns, the translational control mechanisms mediated by eIF2 and eIF4F, and the activation state of nutrient- sensing and insulin receptor signaling pathways in the liver of nondiabetic ..
  32. MARC ARRIBAS-LAYTON; Fiscal Year: 2014
    ..mRNAs in the cytoplasm are associated with a 5'cap, the cap binding translation initiation complex eIF4F, and a polyA tail...
  33. NEW TRANSLATION INITIATION INHIBITORS FOR CANCER THERAPY
    Jose Halperin; Fiscal Year: 2004
    ..The structure and the interactions of many translation initiation factors including eIF4E and eiF4G have been elucidated mainly by Dr. G. Wagner, a third member of this NCDDG...
  34. ANDREW CALEB HSIEH; Fiscal Year: 2016
    ..synthesis including the PI3K-AKT-mTOR and MYC oncogenic signaling pathways converge on the cap-binding complex, eIF4F, to regulate global protein synthesis and the translation of specific mRNAs...
  35. Regulation of Protein Synthesis in Neonatal Sepsis
    RENAN ORELLANA; Fiscal Year: 2009
    ..the phosphorylation of eIF4E, 4E-BP1, p70S6 kinase and protein kinase B (PKB), the association of eIF4E with eIF4G and 4E-BP1, and the activation of NFkappaB signaling...
  36. Bernard Roizman; Fiscal Year: 2014
    ..iv) Earlier reports indicated that VHS binds 3 components of eIF4F complex, i.e. eIF4H, eIF4B and eIF4AII and VP16...
  37. DAVID ERIC WEINBERG; Fiscal Year: 2016
    ..proposal will test the hypothesis that translational efficiency (TE) is largely explained by recruitment of the eIF4F cap- binding complex, which is the first step of initiation...
  38. Characterization of hantavirus N protein-mediated translation mechanism
    MOHAMMAD AYOUB MIR; Fiscal Year: 2013
    ..translation mechanisms known to date, and facilitates the translation of capped mRNAs without the requirement of eIF4F cap binding complex...
  39. Ras-Induced Autophagic Growth-Arrest in Immortalized Human Epithelial Cells
    JOSE GOMEZ-GARCIA; Fiscal Year: 2011
    ..Aim 3. Determine whether activation of c-Myc or eIF4F, suppression of p53 or p16Ink4 - or combinations of these oncogenic interventions - contribute to the cell-context ..
  40. Translational Control of Breast Cancer
    Vitaly A Polunovsky; Fiscal Year: 2010
    ..is exerted by elF4E, which is the rate limiting component of the cap-dependent translation initiation apparatus, eIF4F. When activated by extracellular signals or metabolic cues, elF4E selectively stimulates translation of messages ..
  41. Deborah Silvera; Fiscal Year: 2014
    ..overarching goal of this proposal is to elucidate the role of altered activity of translation initiation complex eIF4F, and the efficacy of inhibition of individual members of the eIF4F complex in the selective translation, survival, ..
  42. Jens Lykke-Andersen; Fiscal Year: 2015
    ..component of the Lsm1-7 complex in decapping and PB formation, 2) How does the Dcp2 decapping complex remodel the eIF4F cap-binding complex to gain access to the mRNA cap, and 3) what is the role of mRNA tailing in decapping and decay ..
  43. Factors affecting mRNA deadenylation
    Clyde L Denis; Fiscal Year: 2010
    ..also established that TIF defects accelerate deadenylation dependent on these two domains of PAB1, and the TIF, eIF4G, make contacts to the RRM1 and P domain...
  44. DAVID MORSE LIVINGSTON; Fiscal Year: 2016
    ..It encodes an 1863 residue nuclear protein, p220. p220 makes contributions to multiple forms of genome integrity control, among which is an ability to support the ..
  45. Matthias Gromeier; Fiscal Year: 2016
    ..2) Elucidate the mechanism of IRES-mediated translation controlled by eIF4G. We identified signal transduction pathways that converge on the central scaffold of the translation apparatus and ..
  46. Eckhard Jankowsky; Fiscal Year: 2015
    ..In Aim 1 we will determine how biochemical characteristics of Ded1p and the interaction with the co-factor eIF4G1 affect Ded1p's function in translation initiation, using a combination of molecular biological and ..
  47. POLIOVIRUS-INDUCED ALTERATIONS IN PROTEIN SYNTHESIS
    DIANE ETCHISON; Fiscal Year: 1991
    ..activation of a protease, the second is the specific cleavage of the cellular cap- binding complex subunit, p220, and the third may be the specific stimulation of viral mRNA translation...
  48. STRUCTURE/FUNCTION OF PICORNAVIRUS RNA GENOMES
    Peter Sarnow; Fiscal Year: 2002
    ..Various forms of the translation initiation factor eIF4G have been implicated to be important in translation initiation by both the conventional 5' end-dependent scanning ..
  49. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..Overall, the research design will establish the mechanism by which myocardial protein synthesis is reduced in response to alcohol abuse. ..
  50. Myocardial Protein Synthesis After Thermal Injury
    Charles Lang; Fiscal Year: 2005
    ..phosphorylation status of the various 4E-binding proteins and p7OS6 kinase, and quantitating the degradation of eIF4G; (4) to determine the mechanism by which burn impairs peptide-chain elongation in heart, by quantitating the rate ..
  51. Antiretroviral-Induced Defects in Muscle Protein Synthesis
    Charles H Lang; Fiscal Year: 2010
    ..Such data is needed to both realize the full potential and avoid possible pitfalls of this drug in the long-term treatment of HIV-infected individuals. ..
  52. Alcohol-Induced Regulation of Hepatic Protein Synthesis
    THOMAS VARY; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  53. MECHANISMS OF TRANSLATION CONTROL DURING APOPTOSIS
    Richard Lloyd; Fiscal Year: 2007
    ..This investigation will focus on eIF4G, 4E-BP1 which regulate mRNA selection and eIF4B, which likely functions in 5'-3' interactions in ..
  54. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    Scot R Kimball; Fiscal Year: 2010
    ....
  55. Analysis of BRCA1/non-coding RNA interactions
    Shihua Lu; Fiscal Year: 2007
    ..The p220 product of BRCA1 is dedicated, in part, to genome integrity control...
  56. The Unfolded Protein Response After Brain Ischemia
    DONALD DEGRACIA; Fiscal Year: 2006
    ..eukaryotic initiation factor 2 (eIF2alpha), and the proteolytic fragmentation of eukaryotic initiation factor 4G (eIF4G)...
  57. Regulation of translation initiation in eukaryotes
    Vincent P Mauro; Fiscal Year: 2010
    ..g. for the production of therapeutic proteins, and new avenues for analyzing the potential contributions of mis-regulation to disease. ..
  58. Role of RNA Structural Switches in the Replication of a (+)-Strand RNA Virus
    ANNE ELIZABETH SIMON; Fiscal Year: 2010
    ....
  59. REGULATION OF ADIPOCYTE PROTEIN SYNTHESIS BY AMINO ACIDS
    Christopher Lynch; Fiscal Year: 2007
    ..The proposed studies will elucidate how leucine regulates mTOR and the roles of leucine stimulation of mTOR in adipose tissue physiology and obesity. ..
  60. Hepatocyte Proliferation During Development: Role of p38
    PHILIP GRUPPUSO; Fiscal Year: 2006
    ..We anticipate that these studies will provide novel insights into the regulation of hepatocyte proliferation during normal development and in the mature rat. ..
  61. Form and Motion Integration
    Anthony Norcia; Fiscal Year: 2008
    ..abstract_text> ..
  62. IGF-1 Receptor Signaling in Mammary Gland Development
    Darryl Hadsell; Fiscal Year: 2006
    ..abstract_text> ..
  63. GERM LINE ESTABLISHMENT IN DROSOPHILA MELANOGASTER
    Paul Lasko; Fiscal Year: 2009
    ..Finally, in the 5th aim the relationship between Tud, a protein essential downstream of Vas for germ line establishment, Cup, a translational regulator, TACC, a microtubule binding protein, and Dynein heavy chain will be examined. ..
  64. STRUCTURE/FUNCTION OF YEAST TRANSLATION FACTOR EIF4G
    Alan Sachs; Fiscal Year: 2001
    ..outlines experiments whose goals are to understand how each of these proteins as well as RNA interacts with yeast eIF4G1 and with each other...
  65. Screening for Small Molecule Inhibitors of Eukaryotic Translation Initiation
    Jerry Pelletier; Fiscal Year: 2007
    ..molecules that alter eIF4F complex integrity, by disrupting the association between two of its subunits, eIF4E and eIF4G. Following the initial identification of compounds that show activity in the HTS assay, false-positives will be ..
  66. Hantavirus RNA Encapsidation
    Antonito Panganiban; Fiscal Year: 2006
    ..abstract_text> ..
  67. NORMAL AND ABNORMAL DEVELOPMENT OF SPATIAL VISION
    Anthony Norcia; Fiscal Year: 2008
    ..This study will also determine whether nasalward/temporal biases previously reported for large field uniform stimuli propagate to the figure/ground segmentation process. ..
  68. Leptin modulation of insulin action in the islet b-cell
    Rohit Kulkarni; Fiscal Year: 2005
    ..abstract_text> ..
  69. Role of Vpu in HIV1 Particle Exit
    Antonito Panganiban; Fiscal Year: 2004
    ..Additional genetic experiments will be carried out to determine the nature of second site revertants that arise following the propagation of primary Vpu mutants. ..
  70. DEVELOPMENT OF FORM, RIVALRY AND BINOCULARITY
    Anthony Norcia; Fiscal Year: 2003
    ....
  71. Cis- and Trans-acting Factors in HCV Gene Expression
    Shelton Bradrick; Fiscal Year: 2008
    ..abstract_text> ..