E2F1

Summary

Gene Symbol: E2F1
Description: E2F transcription factor 1
Alias: E2F-1, RBAP1, RBBP3, RBP3, transcription factor E2F1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, retinoblastoma-associated protein 1, retinoblastoma-binding protein 3
Species: human

Top Publications

  1. pmc E2F-dependent histone acetylation and recruitment of the Tip60 acetyltransferase complex to chromatin in late G1
    Stefan Taubert
    DNAX Research Institute, Palo Alto, California 94304, USA
    Mol Cell Biol 24:4546-56. 2004
  2. pmc Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis
    Piyali Dasgupta
    Department of Interdisciplinary Oncology, H Lee Moffitt Cancer Center and Research Institute, University of South Florida, 12902 Magnolia Dr, Tampa, FL 33612, USA
    Mol Cell Biol 24:9527-41. 2004
  3. ncbi Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter
    H Rotheneder
    Vienna Biocenter, University of Vienna, Austria
    J Mol Biol 293:1005-15. 1999
  4. pmc Cell cycle association of the retinoblastoma protein Rb and the histone demethylase LSD1 with the Epstein-Barr virus latency promoter Cp
    Charles M Chau
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    J Virol 82:3428-37. 2008
  5. doi The dark side of E2F1: in transit beyond apoptosis
    David Engelmann
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, Rostock, Germany
    Cancer Res 72:571-5. 2012
  6. pmc Rb/E2F1 regulates the innate immune receptor Toll-like receptor 3 in epithelial cells
    Manabu Taura
    Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan
    Mol Cell Biol 32:1581-90. 2012
  7. doi E2F1 in melanoma progression and metastasis
    Vijay Alla
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, Rostock, Germany
    J Natl Cancer Inst 102:127-33. 2010
  8. pmc Regulation of the cyclin E gene by transcription factor E2F1
    K Ohtani
    Department of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 92:12146-50. 1995
  9. ncbi Human ARF binds E2F1 and inhibits its transcriptional activity
    B Eymin
    Groupe de Recherche sur le Cancer du Poumon, INSERM EMI 9924, Institut Albert Bonniot, 38706 La Tronche Cedex, France
    Oncogene 20:1033-41. 2001
  10. ncbi Interaction between the pura and E2F-1 transcription factors
    Nune Darbinian
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA
    Anticancer Res 24:2585-94. 2004

Research Grants

  1. Bandana Chatterjee; Fiscal Year: 2016
  2. Kelly L Jordan-Sciutto; Fiscal Year: 2015
  3. The Role of Rb Dephosphorylation in Apoptosis
    Nancy A Krucher; Fiscal Year: 2010
  4. Brian F Clem; Fiscal Year: 2016
  5. Li Wang; Fiscal Year: 2015
  6. Transcriptional Responses in Neurodegenerative Diseases
    Robert Bowser; Fiscal Year: 2007
  7. E2F1 in Cardiac Neovascularization
    Gangjian Qin; Fiscal Year: 2013
  8. Investigating the role of the cell cycle protein E2F1 in HIV-induced neurotoxicit
    Jacob Zyskind; Fiscal Year: 2013
  9. Mip/LIN9 and Cell Proliferation
    Oscar R Colamonici; Fiscal Year: 2012
  10. Nihal Ahmad; Fiscal Year: 2016

Detail Information

Publications257 found, 100 shown here

  1. pmc E2F-dependent histone acetylation and recruitment of the Tip60 acetyltransferase complex to chromatin in late G1
    Stefan Taubert
    DNAX Research Institute, Palo Alto, California 94304, USA
    Mol Cell Biol 24:4546-56. 2004
    ..stimulation, repressive E2F4-p130-histone deacetylase complexes dissociate from, while activating species (E2F1, -2, and -3) associate with, target promoters...
  2. pmc Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis
    Piyali Dasgupta
    Department of Interdisciplinary Oncology, H Lee Moffitt Cancer Center and Research Institute, University of South Florida, 12902 Magnolia Dr, Tampa, FL 33612, USA
    Mol Cell Biol 24:9527-41. 2004
    ..It appears that Raf-1 links mitogenic signaling to Rb and that disruption of this interaction could aid in controlling proliferative disorders...
  3. ncbi Transcription factors of the Sp1 family: interaction with E2F and regulation of the murine thymidine kinase promoter
    H Rotheneder
    Vienna Biocenter, University of Vienna, Austria
    J Mol Biol 293:1005-15. 1999
    ..Co-expression of E2F-1 results in synergistic activation in 3T6 but not in SL2 cells...
  4. pmc Cell cycle association of the retinoblastoma protein Rb and the histone demethylase LSD1 with the Epstein-Barr virus latency promoter Cp
    Charles M Chau
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    J Virol 82:3428-37. 2008
    ..Several previously unknown factors were identified, including the cell cycle regulatory proteins E2F1 and Rb...
  5. doi The dark side of E2F1: in transit beyond apoptosis
    David Engelmann
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, Rostock, Germany
    Cancer Res 72:571-5. 2012
    b>E2F1 plays a critical role in cell-cycle progression and the induction of apoptosis in response to DNA damage. The latest evidence has uncovered that this tumor suppressor is most relevant for cancer progression and chemoresistance...
  6. pmc Rb/E2F1 regulates the innate immune receptor Toll-like receptor 3 in epithelial cells
    Manabu Taura
    Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Kumamoto, Japan
    Mol Cell Biol 32:1581-90. 2012
    ..TLR3 promoter analysis showed that Rb modulates the transcription factor E2F1, which directly binds to the proximal promoter of TLR3...
  7. doi E2F1 in melanoma progression and metastasis
    Vijay Alla
    Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, Rostock, Germany
    J Natl Cancer Inst 102:127-33. 2010
    ..Overexpression of the E2F1 transcription factor is common in high-grade tumors that are associated with poor patient survival...
  8. pmc Regulation of the cyclin E gene by transcription factor E2F1
    K Ohtani
    Department of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 92:12146-50. 1995
    ..We now show that the accumulation of G1 cyclins is regulated by E2F1. E2F binding sites are found in both the cyclin E and cyclin D1 promoters, both promoters are activated by E2F gene ..
  9. ncbi Human ARF binds E2F1 and inhibits its transcriptional activity
    B Eymin
    Groupe de Recherche sur le Cancer du Poumon, INSERM EMI 9924, Institut Albert Bonniot, 38706 La Tronche Cedex, France
    Oncogene 20:1033-41. 2001
    ..Among these is the transcription factor E2F1, a final effector of the RB pathway, that induces ARF expression...
  10. ncbi Interaction between the pura and E2F-1 transcription factors
    Nune Darbinian
    Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA
    Anticancer Res 24:2585-94. 2004
    ..g. pRb, is critical for G1/S progression. However ectopic E2F-1 overexpression activates p53 and inhibits growth. Previously, the multifunctional Pura protein was found to bind to E2F-1 and inhibit E2F-1 transcriptional activity...
  11. pmc Transcription factor E2F-1 is upregulated in human gastric cancer tissues and its overexpression suppresses gastric tumor cell proliferation
    Qiang Xiao
    Department of Surgery, First Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P R China
    Cell Oncol 29:335-49. 2007
    ....
  12. ncbi Transcriptional regulation of a new variant of human platelet-derived growth factor receptor alpha transcript by E2F-1
    Yusuke Minato
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama 223 8522, Japan
    Gene 403:89-97. 2007
    ..Furthermore, epigenetic modulation might be involved in the expression of the type II transcript. Our findings provide new insights into the regulatory mechanism of PDGF receptor alpha transcription in normal and tumor cells...
  13. doi Epigenetic regulation of E2F-1-dependent Bnip3 transcription and cell death by nuclear factor-κB and histone deacetylase-1
    Hongying Gang
    Department of Physiology, Faculty of Medicine, The Institute of Cardiovascular Sciences, St Boniface General Hospital Research Centre, Winnipeg, MB, R2H 2A6, Canada
    Pediatr Cardiol 32:263-6. 2011
    ..This report highlights the intricate nature between the cellular factors E2F-1 and nuclear factor-κB (NF-κB) and the epigenetic regulation of Bnip3 gene transcription by HDAC1 for cell survival of ventricular myocytes...
  14. ncbi Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathway
    Lulu Farhana
    John D Dingell VA Medical Center and Karmanos Cancer Institute, and Department Internal Medicine, Wayne State University, Detroit, Michigan 48201, USA
    Cancer Res 62:3842-9. 2002
    ..Thus, CD437 modulates the expression of E2F-1 and cyclin B through the simultaneous stimulation and inhibition of the cyclin B and E2F-1 E3 ligases, respectively...
  15. doi Acetylation status of E2F-1 has an important role in the regulation of E2F-1-mediated transactivation of tumor suppressor p73
    Toshinori Ozaki
    Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba 260 8717, Japan
    Biochem Biophys Res Commun 386:207-11. 2009
    ..Taken together, our present findings suggest that acetylation status of E2F-1 contributes to the selective activation of its target genes...
  16. pmc Inhibition of aldose reductase prevents growth factor-induced G1-S phase transition through the AKT/phosphoinositide 3-kinase/E2F-1 pathway in human colon cancer cells
    Kota V Ramana
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555 0647, USA
    Mol Cancer Ther 9:813-24. 2010
    ..Mol Cancer Ther; 9(4); 813-24. (c)2010 AACR...
  17. pmc Silencing of sodium-hydrogen exchanger 1 attenuates the proliferation, hypertrophy, and migration of pulmonary artery smooth muscle cells via E2F1
    Lunyin Yu
    Pulmonary and Critical Care Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, 02114 2696, USA
    Am J Respir Cell Mol Biol 45:923-30. 2011
    ..hypoxia-induced proliferation, hypertrophy, and migration of human PASMCs via repression of the nuclear transcription factor E2F1. This study revealed a novel mechanism underlying the regulation of hypoxic pulmonary hypertension and ..
  18. ncbi R-(+)-alpha-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1
    Michaela Artwohl
    Department of Internal Medicine III, Division of Endocrinology and Metabolism, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Am J Physiol Endocrinol Metab 293:E681-9. 2007
    ..LA's ability to inhibit apoptosis and proliferation of ECs could beneficially affect endothelial dysfunction, which precedes manifestation of late diabetic vascular complications...
  19. doi Transcriptional regulation of human fibroblast growth factor receptor 1 by E2F-1
    Mai Kanai
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama, Japan
    Gene 438:49-56. 2009
    ..Because disruption of the pRB/E2F pathway is frequently observed in tumor cells, our findings provide valuable information for studying the role of FGFR 1 in tumor progression...
  20. ncbi The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins
    S B McMahon
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014, USA
    Cell 94:363-74. 1998
    ..These data suggest that TRRAP is an essential cofactor for both the c-Myc and E1A/E2F oncogenic transcription factor pathways...
  21. pmc Analysis of promoter binding by the E2F and pRB families in vivo: distinct E2F proteins mediate activation and repression
    Y Takahashi
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA
    Genes Dev 14:804-16. 2000
    ..These findings suggest that repression and activation of E2F-responsive genes may occur through distinct E2F heterodimers that direct the sequential recruitment of enzymes able to deacetylate and then acetylate core histones...
  22. ncbi DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters
    K D Robertson
    Laboratory of Molecular Embryology, NICHD, NIH, Bethesda, Maryland, USA
    Nat Genet 25:338-42. 2000
    ..mammalian DNA methyltransferase, DNMT1, co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1 and that DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites...
  23. ncbi The MDM2 antagonist nutlin-3 sensitizes p53-null neuroblastoma cells to doxorubicin via E2F1 and TAp73
    Susan K Peirce
    Department of Pediatrics, Division of Hematology and Oncology, Aflac Cancer Center and Blood Disorders Service, Emory University School of Medicine, Atlanta, GA 30322, USA
    Int J Oncol 34:1395-402. 2009
    ..Nutlin-3 treatment upregulated TAp73 and E2F1 protein levels...
  24. pmc E2F1 mediates DNA damage and apoptosis through HCF-1 and the MLL family of histone methyltransferases
    Shweta Tyagi
    Center for Integrative Genomics, University of Lausanne, Genopode, Lausanne, Switzerland
    EMBO J 28:3185-95. 2009
    b>E2F1 is a key positive regulator of human cell proliferation and its activity is altered in essentially all human cancers. Deregulation of E2F1 leads to oncogenic DNA damage and anti-oncogenic apoptosis...
  25. pmc E2F integrates cell cycle progression with DNA repair, replication, and G(2)/M checkpoints
    Bing Ren
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genes Dev 16:245-56. 2002
    ..A subset of these targets was also bound by E2F1. Most previously identified target genes known to have roles in DNA replication and cell cycle control and ..
  26. pmc Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation
    E S Knudsen
    Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093 0322, USA
    Mol Cell Biol 17:5771-83. 1997
    ....
  27. ncbi Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage
    Alessandra Ianari
    Department of Experimental Medicine and Pathology, University of Rome La Sapienza, 00161 Rome, Italy
    J Biol Chem 279:30830-5. 2004
    b>E2F1, a member of the E2F family of transcription factors, plays a pivotal role in controlling both physiological cell-cycle progression and apoptotic cell death in response to DNA damage and oncogene activation...
  28. ncbi E2F1 uses the ATM signaling pathway to induce p53 and Chk2 phosphorylation and apoptosis
    John T Powers
    Department of Carcinogenesis, University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    Mol Cancer Res 2:203-14. 2004
    ..Deregulation of the Rb-E2F1 pathway also results in the activation of p53 and the promotion of apoptosis, and this contributes to the ..
  29. ncbi Transcriptional regulation of AKT activation by E2F
    Marie Chaussepied
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 16:831-7. 2004
    ..Moreover, at least one member of this family, E2F1, can mediate apoptotic cell death...
  30. ncbi Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation
    K Helin
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Genes Dev 7:1850-61. 1993
    ..We suggest that "E2F" is the activity that is formed when an E2F-1-related protein and a DP-1-related protein dimerize...
  31. ncbi Disruption of retinoblastoma protein function by coexpression of its C pocket fragment
    P J Welch
    Department of Biology, University of California at San Diego, La Jolla 92093 0347
    Genes Dev 9:31-46. 1995
    ..These results suggest that the inhibition of RB-binding proteins is not sufficient to suppress cell growth and that the assembly of RB-mediated protein complexes is also important for the promotion of cell-cycle arrest...
  32. ncbi Apaf-1 is a mediator of E2F-1-induced apoptosis
    Yusuke Furukawa
    Division of Stem Cell Regulation, Center for Molecular Medicine and the Department of Biochemistry, Jichi Medical School, Tochigi 329 0498, Japan
    J Biol Chem 277:39760-8. 2002
    ..These data demonstrate a novel mechanism of apoptosis in which an increase in Apaf-1 levels results in direct activation of caspase-9 without mitochondrial damage, leading to the initiation of a caspase cascade...
  33. ncbi Interactions between E2F1 and SirT1 regulate apoptotic response to DNA damage
    Chuangui Wang
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
    Nat Cell Biol 8:1025-31. 2006
    ..This report shows that the cell-cycle and apoptosis regulator E2F1 induces SirT1 expression at the transcriptional level...
  34. doi 8-Chloro-adenosine-induced E2F1 promotes p14ARF gene activation in H1299 cells through displacing Sp1 from multiple overlapping E2F1/Sp1 sites
    Hai Jun Zhang
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Xue Yuan Road 38, Beijing 100083, PR China
    J Cell Biochem 106:464-72. 2009
    ..the idea that the ARF promoter has a unique ability to distinguish between aberrant and physiological levels of E2F1. Therefore, the functional characterization of the promoter still needs to be done...
  35. pmc The retinoblastoma protein binds E2F residues required for activation in vivo and TBP binding in vitro
    C Hagemeier
    Wellcome CRC Institute, Cambridge, UK
    Nucleic Acids Res 21:4998-5004. 1993
    ..Consistent with this model, we find that the TATA box binding protein TBP can bind to the E2F activation domain in vitro in a manner indistinguishable from that of RB...
  36. ncbi Modular organization of the E2F1 activation domain and its interaction with general transcription factors TBP and TFIIH
    A Pearson
    Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
    Oncogene 15:2643-58. 1997
    The transcriptional activator E2F1 regulates the expression of genes at the G1/S boundary...
  37. ncbi Inhibition of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase pathway induces p53-independent transcriptional regulation of p21(WAF1/CIP1) in human prostate carcinoma cells
    S J Lee
    Medicine Branch, Division of Clinical Sciences, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:10618-23. 1998
    ....
  38. pmc Cumulative effect of phosphorylation of pRB on regulation of E2F activity
    V D Brown
    Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Mol Cell Biol 19:3246-56. 1999
    ..Surprisingly, no one CDK site regulated the interaction of pRB with E2F when E2F was bound to DNA. Instead, disruption of transcriptional repression resulted from accumulation of phosphate groups on the RB molecule...
  39. ncbi Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation
    X Xia
    Greenebaum Cancer Center, University of Maryland, Baltimore, Maryland 21201, USA
    J Cell Physiol 187:209-17. 2001
    ..The overexpression of Ebp1 in MCF-7 and AU565 (Rb(+)) cells inhibited the activity of the E2F1 regulated cyclin-E promoter. Ebp1 bound E2F1 indirectly via Rb in lysates of MCF-7 cells...
  40. pmc Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark
    Alexey S Revenko
    Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Sacramento, CA 95817, USA
    Mol Cell Biol 30:5260-72. 2010
    ..We report here that ANCCA directly interacts with E2F1 to E2F3 and that its N terminus interacts with both the N and C termini of E2F1...
  41. pmc E2F-1-mediated transactivation is inhibited by complex formation with the retinoblastoma susceptibility gene product
    E K Flemington
    Division of Tumor Virology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 90:6914-8. 1993
    ..These findings support a model wherein RB suppresses E2F-1-mediated transcriptional activation through direct physical association...
  42. ncbi A common E2F-1 and p73 pathway mediates cell death induced by TCR activation
    N A Lissy
    Howard Hughes Medical Institute, Department of Pathology, Washington University School of Medicine, St Louis Missouri 63110, USA
    Nature 407:642-5. 2000
    ..We conclude that TCR-AICD occurs from a late G1 cell-cycle checkpoint that is dependent on both E2F-1 and p73 activities. These observations indicate that, unlike p53, p73 serves to integrate receptor-mediated apoptotic stimuli...
  43. ncbi A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F
    K Helin
    Massachusetts General Hospital, Charlestown 02129
    Cell 70:337-50. 1992
    ..Here we describe the characterization of a cDNA clone that encodes a protein with properties of E2F. This clone, RBP3, was identified by the ability of its gene product to interact with pRB...
  44. ncbi Phosphorylation of E2F-1 by cyclin A-cdk2
    M Kitagawa
    Banyu Tsukuba Research Institute, Merck Research Laboratories, Japan
    Oncogene 10:229-36. 1995
    ..These results suggest that E2F-1 is phosphorylated by cyclin A-cdk2 in the S phase in vivo as well as in vitro and that its phosphorylation by cyclin A-cdk2 may modulate its activity...
  45. pmc E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle
    C Sardet
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142
    Proc Natl Acad Sci U S A 92:2403-7. 1995
    ..These findings suggest that E2F-4 and E2F-5 may contribute to the regulation of early G1 events including the G0/G1 transition...
  46. pmc Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation
    M Xu
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    Mol Cell Biol 14:8420-31. 1994
    ..Finally, we report that the DNA-binding activity of the E2F-1/DP-1 complex is inhibited following phosphorylation by cyclin A/CDK2...
  47. pmc p53 and E2F-1 cooperate to mediate apoptosis
    X Wu
    Department of Molecular Biology, Princeton University, NJ 08544 1014
    Proc Natl Acad Sci U S A 91:3602-6. 1994
    ..Coexpression of the wild-type p53 protein and E2F-1 in these cells resulted in a rapid loss of cell viability through a process of apoptosis. Thus, the cell cycle utilizes an interacting or communicative pathway between RB-E2F-1 and p53...
  48. pmc Genetic characterization of a mammalian protein-protein interaction domain by using a yeast reverse two-hybrid system
    M Vidal
    Massachusetts General Hospital Cancer Center, Charlestown 02129, USA
    Proc Natl Acad Sci U S A 93:10321-6. 1996
    ..We have selected such mutations in the transcription factor E2F1 that affect its ability to heterodimerize with DP1...
  49. ncbi Interaction between cell cycle regulator, E2F-1, and NF-kappaB mediates repression of HIV-1 gene transcription
    M Kundu
    Center for NeuroVirology and NeuroOncology, and Department of Neurology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 272:29468-74. 1997
    ..The results of this study suggest that NF-kappaB activity may be regulated by its interaction with the cell cycle regulatory protein, E2F-1...
  50. ncbi HIV-1 Tat elongates the G1 phase and indirectly promotes HIV-1 gene expression in cells of glial origin
    M Kundu
    Center for NeuroVirology and NeuroOncology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania 19102, USA
    J Biol Chem 273:8130-6. 1998
    ..The significance of these findings relates to the current hypothesis that indirect effects of HIV-1 infection of the CNS may contribute to the neurological complications associated with AIDS dementia complex...
  51. ncbi Expression of E2F-1 and E2F-4 is reduced in primary and metastatic breast carcinomas
    G H Ho
    Department of Surgery, Singapore General Hospital, Singapore
    Breast Cancer Res Treat 69:115-22. 2001
    ..Our results suggest that not only are the E2Fs likely to function as tumor suppressors in breast cancer, but also that their down-regulation may be important in the development of metastases...
  52. pmc Prohibitin requires Brg-1 and Brm for the repression of E2F and cell growth
    Sheng Wang
    Boston University School of Medicine, Cancer Research Center, 715 Albany Street, Boston, MA 02118, USA
    EMBO J 21:3019-28. 2002
    ....
  53. ncbi Che-1 affects cell growth by interfering with the recruitment of HDAC1 by Rb
    Tiziana Bruno
    Laboratory B, Via delle Messi d Oro 156, 00158 Rome, Italy
    Cancer Cell 2:387-99. 2002
    ..These findings indicate the existence of a pathway of Rb regulation supporting Che-1 as the cellular counterpart of DNA tumor virus oncoproteins...
  54. ncbi The E2F-1 transcription factor is negatively regulated by its interaction with the MDMX protein
    Gordon D Strachan
    Department of Pathology, University of Pennsylvania School of Dental Medicine, 4010 Locust St, Rm 312 Levy Research, Philadelphia 19104 6002, USA
    J Cell Biochem 88:557-68. 2003
    ..These data indicate that the MDMX protein is capable of associating with E2F-1 and negatively regulating its DNA binding ability...
  55. ncbi IFN-gamma inhibits human airway smooth muscle cell proliferation by modulating the E2F-1/Rb pathway
    Yassine Amrani
    Pulmonary, Allergy, and Critical Care Division, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA
    Am J Physiol Lung Cell Mol Physiol 284:L1063-71. 2003
    ....
  56. ncbi Immunohistochemical expression of the transcription factor DP-1 and its heterodimeric partner E2F-1 in non-Hodgkin lymphoma
    Joel A Chan
    Department of Pathology, Los Angeles County and University of Southern California Medical Center, Los Angeles, California, USA
    Appl Immunohistochem Mol Morphol 10:322-6. 2002
    ....
  57. ncbi Chk2 activates E2F-1 in response to DNA damage
    Craig Stevens
    Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow, G12 8QQ, UK
    Nat Cell Biol 5:401-9. 2003
    ..These results suggest a role for E2F-1 in checkpoint control and provide a plausible explanation for the tumour suppressor activity of E2F-1...
  58. ncbi Interaction and functional cooperation of the cancer-amplified transcriptional coactivator activating signal cointegrator-2 and E2F-1 in cell proliferation
    Hee Jeong Kong
    Laboratory of Molecular Growth Regulation, National Institute of Health, Bethesda, MD, USA
    Mol Cancer Res 1:948-58. 2003
    ....
  59. ncbi The emerging role of E2F-1 in the DNA damage response and checkpoint control
    Craig Stevens
    Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK
    DNA Repair (Amst) 3:1071-9. 2004
    ..In addition, emerging information suggests that E2F-1 may play a role in the detection and subsequent repair of damaged DNA...
  60. ncbi HES-1 inhibits 17beta-estradiol and heregulin-beta1-mediated upregulation of E2F-1
    Johan Hartman
    Center for Biotechnology, Department of Biosciences, Karolinska Institutet, Novum, S 141 57 Huddinge Sweden
    Oncogene 23:8826-33. 2004
    ....
  61. ncbi Apoptosis signal-regulating kinase 1 is a direct target of E2F1 and contributes to histone deacetylase inhibitor-induced apoptosis through positive feedback regulation of E2F1 apoptotic activity
    Jing Tan
    Laboratory of Molecular Pharmacology, Microarray and Information and Mathematical Sciences, Genome Institute of Singapore, Genome Building, 02 01, 60 Biopolis Street, Singapore 138672
    J Biol Chem 281:10508-15. 2006
    ..We have previously shown that histone deacetylase inhibitors (HDACIs) activate the E2F1-Bim apoptotic pathway, leading to efficient cell killing in cancer cells with deregulated E2F1 activity...
  62. ncbi E2F-1 is a critical modulator of cellular senescence in human cancer
    Chaehwa Park
    Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135 710, Korea
    Int J Mol Med 17:715-20. 2006
    ..Herein we show that E2F1, a transcription factor essential to a cell cycle progress and a main target of tumor suppressor Rb, is a critical ..
  63. ncbi p14ARF regulates E2F-1 ubiquitination and degradation via a p53-dependent mechanism
    Helen Rizos
    Westmead Institute for Cancer Research, University of Sydney at Westmead Millennium Institute, Westmead Hospital, Westmead, Australia
    Cell Cycle 6:1741-7. 2007
    ..Importantly, these data establish that the ARF-E2F-1 pathway is an extension of the p53-mdm2-ARF tumor suppressor network and is unlikely to constitute a p53-independent pathway for ARF function...
  64. ncbi Dual specificity phosphatase 1/CL100 is a direct transcriptional target of E2F-1 in the apoptotic response to oxidative stress
    Jianli Wang
    Department of Radiation Oncology, Center for Radiological Research, College of Physicians and Surgeons, Columbia University Medical Center, 630 West 168th Street, New York, NY 10032, USA
    Cancer Res 67:6737-44. 2007
    ..Interestingly, E2F-1 is acetylated following oxidative stress. Our findings show that E2F-1 is a transcriptional activator of DUSP1 and that DUSP1 is a link between E2F-1 and MAP kinases...
  65. ncbi LEF-1 activates the transcription of E2F1
    Fangfang Zhou
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Biochem Biophys Res Commun 365:149-53. 2008
    ..The present study shows for the first time that LEF-1 associates with E2F1 and further beta-catenin independently activates the E2F-responsive reporter gene by attenuating the interaction ..
  66. doi TGF-beta induces growth arrest in Burkitt lymphoma cells via transcriptional repression of E2F-1
    Lindsay C Spender
    Growth Factor Signalling Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, United Kingdom
    J Biol Chem 284:1435-42. 2009
    ..These data indicate that deregulation of c-MYC in lymphoma cells does not overcome the tumor suppressor function of TGF-beta and that repression of E2F-1 transcription is sufficient for the efficient induction of cytostasis...
  67. pmc CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability
    Anne Sophie Huart
    Cancer Research UK p53 Signal Transduction Group, University of Edinburgh, Institute of Genetics and Molecular Medicine, Division of Cancer Biology, Crewe Road South, Edinburgh EH4 2XR, Scotland, United Kingdom
    J Biol Chem 284:32384-94. 2009
    ....
  68. pmc In vivo association of E2F and DP family proteins
    C L Wu
    Massachusetts General Hospital Cancer Center, Charlestown 02129, USA
    Mol Cell Biol 15:2536-46. 1995
    ..However, the various E2F/DP complexes display strong differences in the ability to bind to either pRB or p107 in vivo, and the specificity of pRB or p107 binding is mediated by the E2F subunit...
  69. ncbi Differential regulation of E2F transactivation by cyclin/cdk2 complexes
    B D Dynlacht
    Massachusetts General Hospital Cancer Center, Charlestown 02129
    Genes Dev 8:1772-86. 1994
    ..Thus, this in vitro transcriptional assay allows activation and inactivation of E2F transcription, and our findings demonstrate how transcriptional regulation of E2F can be linked to cell cycle-dependent activation of kinases...
  70. ncbi E2F-4, a new member of the E2F transcription factor family, interacts with p107
    D Ginsberg
    Dana Farber Cancer Institute, Boston, Massachusetts
    Genes Dev 8:2665-79. 1994
    ..p107 binding not only can be linked to the regulation of E2F-4 transcriptional activity, but also to suppression of the ability of E2F-4 to transform an immortalized rodent cell line...
  71. pmc E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry
    K Moberg
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Mol Cell Biol 16:1436-49. 1996
    ..Despite this, a considerable amount of E2F-4 exists as free E2F. In G1 cells, this accounts for almost all of the free activity. Once the cells enter S phase, free E2F is composed of an equal mixture of E2F-4 and E2F-1...
  72. pmc DP-2, a heterodimeric partner of E2F: identification and characterization of DP-2 proteins expressed in vivo
    K T Rogers
    Department of Molecular Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:7594-9. 1996
    ..Thus, we conclude that DP-2 encodes a set of structurally, and perhaps functionally, distinct proteins in vivo...
  73. pmc The CBP co-activator stimulates E2F1/DP1 activity
    D Trouche
    Wellcome CRC Institute and Department of Pathology, University of Cambridge, UK
    Nucleic Acids Res 24:4139-45. 1996
    The cell cycle-regulating transcription factors E2F1/DP1 activate genes whose products are required for S phase progression...
  74. pmc Unusual proliferation arrest and transcriptional control properties of a newly discovered E2F family member, E2F-6
    S Gaubatz
    Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 95:9190-5. 1998
    ..Taken together, these data suggest that E2F-6 can regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression...
  75. pmc TRIP-Br: a novel family of PHD zinc finger- and bromodomain-interacting proteins that regulate the transcriptional activity of E2F-1/DP-1
    S I Hsu
    Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
    EMBO J 20:2273-85. 2001
    ....
  76. ncbi E2F-1 gene therapy induces apoptosis and increases chemosensitivity in human pancreatic carcinoma cells
    Mary Jane Elliott
    Department of Surgery, University of Louisville, James Graham Brown Cancer Center, Louisville, KY, USA
    Tumour Biol 23:76-86. 2002
    ..ROS and etoposide, when combined with E2F-1 overexpression, induce apoptosis in an additive manner. This chemogene combination may provide a potentially useful therapeutic strategy for advanced pancreatic cancer...
  77. pmc Identification of E-box factor TFE3 as a functional partner for the E2F3 transcription factor
    Paloma H Giangrande
    Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 23:3707-20. 2003
    ..In contrast, neither E2F1 nor E2F2 interacted with the p68 promoter under these conditions...
  78. ncbi Differential regulation of E2F1 apoptotic target genes in response to DNA damage
    Natalia Pediconi
    Laboratory of Gene Expression, Fondazione Andrea Cesalpino, University of Rome La Sapienza, Viale del Policlinico 155, 00161 Rome, Italy
    Nat Cell Biol 5:552-8. 2003
    b>E2F1, a member of the E2F family of transcription factors, in addition to its established proliferative effect, has also been implicated in the induction of apoptosis through p53-dependent and p53-independent pathways...
  79. ncbi Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence
    Masashi Narita
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 113:703-16. 2003
    ..These results provide a molecular explanation for the stability of the senescent state, as well as new insights into the action of Rb as a tumor suppressor...
  80. ncbi ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain
    Motoko Unoki
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4 6 1 Shiorokanedai, Minato ku, Tokyo 108 8639, Japan
    Oncogene 23:7601-10. 2004
    ..The data reported here suggest that ICBP90 is involved in cell proliferation by way of methylation-mediated regulation of certain genes...
  81. ncbi The central acidic domain of MDM2 is critical in inhibition of retinoblastoma-mediated suppression of E2F and cell growth
    Patima Sdek
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    J Biol Chem 279:53317-22. 2004
    ..Taken together, these results suggest that MDM2-mediated inhibition of Rb likely contributes to MDM2 oncogenic activity...
  82. ncbi E2F-1 transcription factor immunoexpression is inversely associated with tumor growth in colon adenocarcinomas
    John Bramis
    First Department and 4Second Department of Surgery, Laiko Hospital, Medical School of Athens, Athens, Greece
    Anticancer Res 24:3041-7. 2004
    ..001) and positively associated with AI (p = 0.013). In conclusion, we suggest a tumor-suppressive behavior of E2F-1 in colon carcinomas...
  83. ncbi Rb inactivation leads to E2F1-mediated DNA double-strand break accumulation
    M T Pickering
    Department of Molecular Genetics and Microbiology, Program in Immunology and Virology, UMass Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Oncogene 25:746-55. 2006
    ..We show here that pRb inactivation and the resultant deregulation of one E2F family member, E2F1, leads to DNA double-strand break (DSB) accumulation in normal diploid human cells...
  84. ncbi E2F1 induces MRN foci formation and a cell cycle checkpoint response in human fibroblasts
    F M Frame
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, 01655, USA
    Oncogene 25:3258-66. 2006
    Deregulation of the Rb/E2F pathway in human fibroblasts results in an E2F1-mediated apoptosis dependent on Atm, Nbs1, Chk2 and p53...
  85. ncbi Increased gene copy number of the transcription factor E2F1 in malignant melanoma
    Mark A Nelson
    Department of Pathology, College of Medicine, Arizona Cancer Center, University of Arizona, Tucson, Arizona 87515, USA
    Cancer Biol Ther 5:407-12. 2006
    ..We have previously shown a unique translocation in malignant melanoma cells der(12)t(12;20). The transcription factor E2F1 maps to 20q11...
  86. ncbi Stimulation of steroid receptor coactivator-3 (SRC-3) gene overexpression by a positive regulatory loop of E2F1 and SRC-3
    Paola Mussi
    Department of Molecular and Cellular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030, USA
    Mol Endocrinol 20:3105-19. 2006
    ..1, or ACTR) is a transcriptional coactivator for nuclear receptors and certain other transcription factors such as E2F1. SRC-3 is overexpressed in breast cancers, and its overexpression is sufficient to cause mammary carcinomas in vivo...
  87. ncbi E2F-1, Skp2 and cyclin E oncoproteins are upregulated and directly correlated in high-grade neuroendocrine lung tumors
    C Salon
    Equipe Bases Moléculaires de la Progression des Cancers du Poumon, Centre de Recherche INSERM U823, Institut Albert Bonniot, Grenoble Cedex, France
    Oncogene 26:6927-36. 2007
    ..0001) and E2F-1 (P=0.0001) status in NE lung tumors. Overall, our data provide the first evidence of a direct and functional interconnection between the E2F-1, Skp2 and cyclin E oncoproteins in HGNE lung carcinomas...
  88. ncbi Estrogen-regulated gene networks in human breast cancer cells: involvement of E2F1 in the regulation of cell proliferation
    Joshua D Stender
    Department of Biochemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801 3704, USA
    Mol Endocrinol 21:2112-23. 2007
    ..Biochemical analyses confirmed that E2F1 and E2F downstream target genes were increased in MCF-7 and decreased in 231ER+ cells upon estrogen treatment...
  89. ncbi Significance of E2F-1 overexpression in epithelial ovarian cancer
    D S Suh
    Department of Obstetrics and Gynecology, Medical Research Institute, School of Medicine, Pusan National University, Busan, Republic of Korea
    Int J Gynecol Cancer 18:492-8. 2008
    ..The results suggest that E2F-1 has a growth-promoting effect in EOC and that E2F-1 overexpression may provide a useful prognostic indicator for EOC...
  90. ncbi Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappaB upon TLR4 activation
    Ching Aeng Lim
    Laboratory of Immunology and Virology, Genome Institute of Singapore, 138672 Singapore
    Mol Cell 27:622-35. 2007
    ..human monocytic cells, and together with global gene expression profiling, found an overrepresentation of the E2F1-binding motif among RELA-bound loci associated with NF-kappaB target genes...
  91. doi E2F1-regulated microRNAs impair TGFbeta-dependent cell-cycle arrest and apoptosis in gastric cancer
    Fabio Petrocca
    Department of Molecular Virology, Immunology and Medical Genetics, Human Cancer Genetics Program, Ohio State University, 460 West 12th Avenue, Columbus, OH 43210, USA
    Cancer Cell 13:272-86. 2008
    Deregulation of E2F1 activity and resistance to TGFbeta are hallmarks of gastric cancer. MicroRNAs (miRNAs) are small noncoding RNAs frequently misregulated in human malignancies...
  92. doi A role for Myc in facilitating transcription activation by E2F1
    J Y Leung
    Department of Molecular Genetics and Microbiology, Duke Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, NC 27710, USA
    Oncogene 27:4172-9. 2008
    ..b>E2F1 in particular is required for the initial entry to the cell cycle from a quiescent state and is required for the ..
  93. pmc miR-17 and miR-20a temper an E2F1-induced G1 checkpoint to regulate cell cycle progression
    M T Pickering
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 1655, USA
    Oncogene 28:140-5. 2009
    ..due to an accumulation of DNA double-strand breaks, resulting from premature temporal accumulation of the E2F1 transcription factor...
  94. doi The c-MYC-interacting proapoptotic tumor suppressor BIN1 is a transcriptional target for E2F1 in response to DNA damage
    E K Cassimere
    Division of Cancer Biology, Department of Pathology, School of Medicine and Stanley S Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    Cell Death Differ 16:1641-53. 2009
    The E2F1 transcription factor, which was originally identified as a cell-cycle initiator, mediates apoptosis in response to DNA damage...
  95. pmc miR-449a and miR-449b are direct transcriptional targets of E2F1 and negatively regulate pRb-E2F1 activity through a feedback loop by targeting CDK6 and CDC25A
    Xiaojing Yang
    Cancer Biology and Pharmacology, Genome Institute of Singapore, A STAR Agency for Science, Technology, and Research, Singapore 138672
    Genes Dev 23:2388-93. 2009
    ..Here we report that E2F1 directly transactivates miR-449a/b...
  96. pmc RB·E2F1 complex mediates DNA damage responses through transcriptional regulation of ZBRK1
    Ching Chun Liao
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    J Biol Chem 285:33134-43. 2010
    ..We found that the ZBRK1 promoter contains an authentic E2F-recognition sequence that specifically binds E2F1, but not E2F4 or E2F6, together with chromatin remodeling proteins CtIP and CtBP to form a repression complex that ..
  97. doi Direct repression of the human IRF-3 promoter by E2F1
    Hua Guo Xu
    Department of Pediatrics, The First Affiliated Hospital, Nanjing Medical University, 300 Guang Zhou Road, Nanjing, Jiangsu Province 210029, China
    Immunogenetics 63:189-96. 2011
    ..Here we report that the transcription factor E2F1 negatively regulates the basal transcriptional activity of IRF-3 and deregulates IRF-3 expression at ..
  98. doi MiR-15 and miR-16 are direct transcriptional targets of E2F1 that limit E2F-induced proliferation by targeting cyclin E
    Matan Ofir
    The Mina and Everard Goodman Faculty of Life Science, Bar Ilan University, Ramat Gan 52900, Israel
    Mol Cancer Res 9:440-7. 2011
    ..we evidence that the miR-15a, miR-16-1 cluster and related miR-15b, miR-16-2 cluster comprise miRs regulated by E2F1, a pivotal transcription factor that can induce both proliferation and cell death...
  99. doi MiR-106a inhibits glioma cell growth by targeting E2F1 independent of p53 status
    Guang Yang
    Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, People s Republic of China
    J Mol Med (Berl) 89:1037-50. 2011
    ..that the low expression of miR-106a in human glioma specimens is significantly correlated with high levels of E2F1 protein and high-grade glioma...
  100. ncbi E2F: a link between the Rb tumor suppressor protein and viral oncoproteins
    J R Nevins
    Howard Hughes Medical Institute, Section of Genetics, Duke University Medical Center, Durham, NC 27710
    Science 258:424-9. 1992
    ....
  101. ncbi Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
    W G Kaelin
    Dana Farber Cancer Institute, Boston, Massachusetts
    Cell 70:351-64. 1992
    ..Therefore, RBAP-1 is a species of E2F and can bind specifically to the RB pocket...

Research Grants63

  1. Bandana Chatterjee; Fiscal Year: 2016
    ..Since metastatic prostate cancer is often associated with elevated levels of E2F1, which is a key cell cycle regulatory transcription factor, we designed this project to identify regulatory factors ..
  2. Kelly L Jordan-Sciutto; Fiscal Year: 2015
    ..In support of our hypothesis, we have observed increased E2F1 and hyper-phosphorylated pRb in the CNS of patients with HIV encephalitis, in a simian model of disease, and in ..
  3. The Role of Rb Dephosphorylation in Apoptosis
    Nancy A Krucher; Fiscal Year: 2010
    ..Finally we will investigate whether the transcription factor E2F1 plays a critical role in apoptosis induced by the cleavage of Rb...
  4. Brian F Clem; Fiscal Year: 2016
    ..2. To determine the effects of Rb1 deletion on glucose/glutamine metabolism and growth of lung adenocarcinomas in vivo. 3. To correlate the loss of Rb function with changes in the 13C-glucose utilization by human lung tumors in vivo. ..
  5. Li Wang; Fiscal Year: 2015
    ..preliminary results generated during the current grant cycle, we have uncovered a novel role for the transcription factor E2F1 in the regulation of bile acid metabolism...
  6. Transcriptional Responses in Neurodegenerative Diseases
    Robert Bowser; Fiscal Year: 2007
    ..Key protein components of the cell cycle regulated death of neurons include p53, E2F1 and the retinoblastoma protein (pRb)...
  7. E2F1 in Cardiac Neovascularization
    Gangjian Qin; Fiscal Year: 2013
    ..The E2F transcription factors are key regulators of cell growth and survival. Specifically, E2F1 is a transcriptional activator that, when overexpressed, induces quiescent cells to proliferate...
  8. Investigating the role of the cell cycle protein E2F1 in HIV-induced neurotoxicit
    Jacob Zyskind; Fiscal Year: 2013
    ..involves activation of the cell cycle regulatory machinery, notably increased expression of the transcription factor E2F1, which is known to activate transcription of genes necessary for G1 to S phase progression of the cell ..
  9. Mip/LIN9 and Cell Proliferation
    Oscar R Colamonici; Fiscal Year: 2012
    ..CDK4,6 and CDK2 are responsible for the inactivation of the repressor activity that pRB/E2F1-3 and p107,p130/E2F4,5 exert on genes required for cell cycle progression...
  10. Nihal Ahmad; Fiscal Year: 2016
    ..role in melanocytic transformation and melanoma survival via modulating its downstream regulation of p53, FoxO, and E2F1 transcription factors...
  11. Expression of Simiam Virus 40 T Antigens in Intestine
    James M Pipas; Fiscal Year: 2013
    ..cells;(2) assess the mechanisms allowing cell proliferation to proceed in normal crypts in the absence of E2F1-2-3 and, (3) determine whether the activating E2Fs (E2F1, E2F2, and E2F3a) required to establish repression of E2F-..
  12. David G Johnson; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): The E2F1 transcription factor regulates the expression of genes involved in cell cycle progression, apoptosis and differentiation...
  13. ALAN IRA FADEN; Fiscal Year: 2016
    ..of secondary injury following TBI;based upon our research and that from other groups, we hypothesize that E2F 1, 2, and 3 are involved in both cell cycle-related neuronal death (CRND) and microglial activation after brain ..
  14. Weei Chin Lin; Fiscal Year: 2016
    The transcription factor E2F1 plays a pivotal role in the control of cell proliferation and apoptosis...
  15. Thea D Tlsty; Fiscal Year: 2014
    ..Repression of p16INK4a activity in HMEC increases expression of the E2F1 transcription factor and, consequently, of several of its downstream targets such as the chromatin remodeling ..
  16. Weei Chin Lin; Fiscal Year: 2014
    ..data accumulated in our lab demonstrate a role for TopBP1 in controlling two critical transcriptional factors E2F1 and p53, especially their pro-apoptotic activities...
  17. Mechanisms of Pituitary Tumorigenesis
    Shlomo Melmed; Fiscal Year: 2012
    ..In the third Aim we will study mechanisms for Rb/E2F induction of pituitary tumors by assessing E2F1 induction of Pttg transcription and protein expression in Rb mice, in mutant human cancer cells devoid of p53 or ..
  18. OVARIAN STEROID REGULATION OF SEROTONIN IN PRIMATES
    CYNTHIA LOUISE BETHEA; Fiscal Year: 2010
    ..actions in serotonin neurons by characterizing the localization and hormonal regulation of two genes, E2F1, a pivotal transcription factor, and the GABA-Aa3 subunit...
  19. E2F-mediated Control of Vascular Growth and Remodeling
    Bruce Sullenger; Fiscal Year: 2006
    ..The first subclass, containing E2F1, E2F2 and E2F3, are transcriptional activators that induce quiescent cells to proliferate...
  20. Rakesh Kumar; Fiscal Year: 2014
    ..In addition, we will test the hypothesis that E2F1 and HSF1 transcriptional factors are determinants of Pak1 functions at the G1/S phase transition and during mitosis ..
  21. Timothy C Hallstrom; Fiscal Year: 2016
    ..Little is known about the molecular circuitry that tips E2F1 balance towards proliferation in some settings (normal growth), apoptosis in others (oncogenic stress/incipient ..
  22. Mechanisms of Tumor Suppression by ARF
    Pradip Raychaudhuri; Fiscal Year: 2009
    ..ARF induces proteolysis of E2F1 and DP1;and sequesters c-Myc in the nucleolus...
  23. Gender-Specific Role of p202 in Lupus Susceptibility
    Divaker Choubey; Fiscal Year: 2010
    ..The p202 protein (~52-k Da) is an inducible modulator of transcriptional activities of factors, such as p53, E2F1, NF-:B, and AP-1...
  24. Daitoku Sakamuro; Fiscal Year: 2015
    ..Bin1 and PARP-1 expression, we will determine how c-Myc interferes with the transcriptional activities of Miz-1 and E2F1, two transcription factors that separately activate the Bin1 promoter, and conversely, how c-Myc upregulates PARP-1 ..
  25. Deborah E Sullivan; Fiscal Year: 2014
    ..The transcription factor E2F1 that is activated by HCMV UL97 is postulated to be key to the convergent regulation of these pathways...
  26. Role of TRIP6 in Malignant Glioma Progression
    Fang Tsyr Lin; Fiscal Year: 2010
    ..In addition, TRIP6 is capable of shuttling to the nucleus to serve as a coactivator of NF-?B, AP-1 and E2F1 in the transcriptional regulation of genes involved in anti-apoptosis and cell growth...
  27. FUNCTION OF THE RETINOBLASTOMA PROTEIN
    Edward Harlow; Fiscal Year: 2002
    ..We have finished the initial characterization of these differences and in the next round of this grant will investigate the functional differences between pRB p107 and p130 in adipogenesis and in other defined differentiation systems. ..
  28. Mechanisms of Increasing Apoptosis Regulation in Developing and Mature Neurons
    Mohanish Deshmukh; Fiscal Year: 2011
    ..In Specific Aim 3 we will test whether the cell cycle- related protein E2F1, which is known to be expressed in dying neurons, is important for restoring Apaf-1 expression and permitting ..
  29. Wenyi Wei; Fiscal Year: 2016
    ..We also found that Cdh1 could affect Rb/E2F1 function...
  30. Functional roles for E2F1 in neurons: mechanisms of synaptic damage in HIV-associ
    JENHAO HARRY TING; Fiscal Year: 2012
    ..Interestingly, the cell cycle machinery, specifically E2F1, has been implicated in many neurodegenerative diseases including HAND...
  31. PTEN Deficiency and Tumor Development
    Yuxin Yin; Fiscal Year: 2012
    ..Identification of the PTEN-mitotic pathway may offer a profound implication for development of therapeutic strategies against tumorigenesis. ..
  32. Proliferation-promoting activities of pRB
    Neetu Singh; Fiscal Year: 2013
    ..Among the functions of pRB is its ability to bind E2Fs and to inhibit E2F1-mediated transcription...
  33. GOVINDASWAMY CHINNADURAI; Fiscal Year: 2015
    ....
  34. Rajesh Agarwal; Fiscal Year: 2014
    ..CPD) positive cells and further enhances p53 protein level in mouse skin epidermal cell, and selectively increases E2F1 protein level in chronic UVB exposed mouse skin...
  35. ANALYSIS OF P73 FUNCTION AND REGULATION BY E2F 1
    Meredith Irwin; Fiscal Year: 2001
    ..Ultimately, the results of the studies proposed in this application may help in the development of novel gene therapies in which the E2F-1 protein, or perhaps, p73, can be used to induce tumor cell apoptosis. ..
  36. Transcriptional Regulation of Estrogen Receptor (ER) by CARM1
    Wei Xu; Fiscal Year: 2012
    ..methyltransferase 1, is involved in the activation of a number of transcriptional factors, including NF-?B, p53, E2F1 and steroid receptors, among which transcriptional activation of estrogen receptors (ERs) by CARM1 is best ..
  37. DNA Methylation in Photoreceptor-Specific Gene Expression and Retinal Disease
    Shannath L Merbs; Fiscal Year: 2010
    ..preliminary studies show that, in the adult mouse, the region around the transcription start site of murine genes Rbp3 (PR-specific) and Rho (rod-specific) is hypomethylated in expressing photoreceptor cells and methylated in non-..
  38. The Role of E2F1 in UV-induced DNA Damage Response
    THOMAS BERTON; Fiscal Year: 2009
    ..Recently, E2F1 has been shown to play a role in the response to DNA damage...
  39. A Luciferase Fusion Library of High Throughput Profiling of Protein Stability
    Gang Lu; Fiscal Year: 2013
    ....
  40. Analysis and Intranasal Treatment of the E2F1 Neurodegenerative Paradigm
    David Marks; Fiscal Year: 2010
    ..Success of this research could revolutionize neurological therapy, and make safe, reliable and low-cost treatment options available to millions of people in the United States. ..
  41. REGULATION OF CELL STRUCTURE AND GROWTH BY E2F1
    David Hall; Fiscal Year: 2000
    ..of this proposal is to understand how cell cycle transit and cellular phenotype is controlled by the transcription factor E2F1. E2F1 is normally expressed at the end of GO/G1 phase and regulates genes whose products are needed for ..
  42. PROLIFERATION & MALIGNANT TRANSFORMATION OF MELANOCYTES
    Ruth Halaban; Fiscal Year: 2004
    ..this end we will infect wild-type or knockout mouse melanocytes, deleted for CKI p16, p2l or p27, or the transcription factor E2F1 with a tetracycline(tet)-inducible retroviral vector encoding bFGF or E2F...
  43. Lung Cancer Diagnostic Test Validation in FNA Specimens
    James Willey; Fiscal Year: 2004
    ..Using these methods in preliminary tests, the E2F1 x c-myc/p21 interactive gene expression index distinguished 28 primary lung cancer samples from 27 primary normal ..
  44. Tumor Suppressor and Oncogenic Pathways in the Placenta
    Lizhao Wu; Fiscal Year: 2007
    ..3. To examine the genetic regulatory networks employed by Rb in the placenta. E2F1, E2F2, E2F3, and Id2 can mediate Rb function in vivo, and concomitant genetic deletion of Rb and either of E2F1, ..
  45. Telomere homolog oligonucleotides for cancer therapy
    Barbara Gilchrest; Fiscal Year: 2004
    ..response and tumor suppressor pathways in cells, including those mediated by ATM, p53, p73, p95/Nbs-1, BRACA1, E2F1 and p16...
  46. CARCINOGENS AND CHROMATIN STRUCTURE AND FUNCTION
    Mark Smulson; Fiscal Year: 2002
    ....
  47. GENE EXPRESSION INDICES IN CHEMORESISTANT LUNG CANCER
    James Willey; Fiscal Year: 2003
    ..AIM 4) Automate the quantitative RT-PCR method. AIM 5) Develop an internet based databank for storage of the data acquired during this study and for storage of data acquired by other laboratories. ..
  48. DNA Damage Retinoblastoma and Cell Survival
    Arun Fotedar; Fiscal Year: 2006
    ..The relationship between the Rb-LxCxE interaction and other Rb binding proteins in the Rb survival pathway will also be clarified. These studies will impact our understanding of Rb in its cell biological functions and in human cancer. ..
  49. Regulation and Function of ECK in Apoptosis
    Yuxin Yin; Fiscal Year: 2005
    ..Characterization of ECK as a cell death receptor may reveal a new target for gene therapy or lead to the development of effective chemotherapeutical strategies in cancer treatment. ..
  50. Role of E2F1 in eugenol-mediated antiproliferative activity in melanoma cells
    Rita Ghosh; Fiscal Year: 2009
    ..i) the safest and most effective dose of eugenol will depend on the route through which it is dispensed and (ii) E2F1 is a eugenol target in melanoma cells...
  51. Rapamycin Potentiates TGFb Tumor Suppressor Function
    Brian Law; Fiscal Year: 2007
    ..growth arrest of normal mammary epithelial cells, and restores TGFbeta-induced cell cycle arrest in Myc, Ras, and E2F1 transformed epithelial cells...
  52. BIOLOGICAL ACTIVITIES OF E2F FAMILY MEMBERS
    David Johnson; Fiscal Year: 2001
    ....
  53. INTERFERON, ANIMAL VIRUSES & PROTEIN SYNTHESIS
    Peter Lengyel; Fiscal Year: 2000
    ..be correlated with the inhibition of the activity of several transcription factors, including c-Fos, c-Jun, AP2, E2F1, E2F4, MyoD, myogenin, NF-kB p50 and 65, p53 and c-Myc...
  54. INTERFERONS AND CELL GROWTH REGULATION
    Divaker Choubey; Fiscal Year: 2000
    ..For this purpose, we plan to localize the growth-inhibitory domain in p202. The proposed studies will contribute to our understanding of the cell growth-inhibitory activities of the IFNs. ..
  55. FUNCTION OF HUMAN DAMAGED DNA BINDING PROTEIN (DDB)
    Stuart Linn; Fiscal Year: 2007
    ..It binds to DNA damages and interacts with a number of protein complexes, including E2F1, p300, STAGA and DNA polymerase epsilon, as well as with transactivator proteins of transforming viruses, including ..
  56. An ORFeome fusion library approach to studying ubiquitylation
    Arthur Young; Fiscal Year: 2007
    ..In our future directions we discuss approaches to characterize proteins identified in our screens, as well as techniques to identify E3 ligases that regulate known substrates. [unreadable] [unreadable] [unreadable] [unreadable]..
  57. Death Signals and Cell Cycle Control
    IAN CRISPE; Fiscal Year: 2005
    ..If the hypothesis is substantiated, E2F-4 and E2F-1 will have been identified as specific vital targets, important in passive and activation-induced T cell death respectively. ..
  58. E2F AND APOPTOSIS
    Timothy Kowalik; Fiscal Year: 2004
    ..In support of this relationship, we have previously shown that expression of E2F1 not only leads to S phase induction, but also to a p53-dependent apoptosis...
  59. E2F-1 Cancer Gene Therapy
    Kelly McMasters; Fiscal Year: 2005
    ..These studies will enhance our understanding of E2F-1-mediated cell death and facilitate the design of improved treatment strategies...
  60. Transcriptional Control of Mcl-1 and Bok/Mtd by E2F1
    WILLIAM CRESS; Fiscal Year: 2007
    ..In contrast to p53, active E2F1 protein is frequently over expressed in cancer cells...
  61. E2F AND THE CONTROL OF APOPTOSIS
    Gustavo Leone; Fiscal Year: 2003
    ..The proposed studies will be directed at understanding the role and the mechanism of action of E2F1, E2F2 and E2F3 in the regulation of cell death, and how other signal transduction pathways important for normal ..
  62. Bin1 in E2F1-Mediated Tumor Growth Suppression
    ERICA KINNEY; Fiscal Year: 2006
    ..thesis project is to define the pathophysiological mechanisms underlying tumor suppression mediated by Bin1 and the E2F1 transcription factor at the molecular and cellular levels...
  63. E2F AND DIFFERENTIATION, APOPTOSIS, AND LEUKEMIA
    SCOTT HIEBERT; Fiscal Year: 2000
    ..Primary cultures of hematopoietic progenitor cells will be used to test the hypothesis that suicide pathways must be inactivated prior to E2F activation in multistep tumorigenesis. ..