DTNB

Summary

Gene Symbol: DTNB
Description: dystrobrevin beta
Alias: dystrobrevin beta, DTN-B, beta-dystrobrevin
Species: human

Top Publications

  1. ncbi Role of -SH groups in rat sugar intestinal transport in vivo
    N Diez
    Departamento de Fisiología y Nutrición, Universidad de Navarra, Pamplona, Spain
    Rev Esp Fisiol 48:127-32. 1992
  2. pmc Cloning and expression of the gene encoding the Thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and biochemical characterization of the enzyme
    D S Burdette
    Biochemistry Department, Michigan State University, East Lansing 48824, USA
    Biochem J 316:115-22. 1996
  3. ncbi Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex
    E Sundstrom
    Division of Geriatric Medicine, Neurotec, Karolinska Institutet, Stockholm, Sweden
    Pharmacol Toxicol 88:342-8. 2001
  4. ncbi Interleukin-8, nitric oxide and glutathione status in proliferative vitreoretinopathy and proliferative diabetic retinopathy
    Erdogan Cicik
    Department of Ophthalmology, Istanbul University, Cerrahpasa Medical Faculty, Istanbul, Turkey
    Ophthalmic Res 35:251-5. 2003
  5. ncbi Potentiation of acid-sensing ion channels by sulfhydryl compounds
    Jun Hyeong Cho
    Dept of Neuroscience, The Ohio State University, Columbus, OH 43210, USA
    Am J Physiol Cell Physiol 292:C2161-74. 2007
  6. doi MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation
    Angela Lanciotti
    Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Neurobiol Dis 37:581-95. 2010
  7. ncbi beta-dystrobrevin, a new member of the dystrophin family. Identification, cloning, and protein associations
    M F Peters
    Department of Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7545, USA
    J Biol Chem 272:31561-9. 1997
  8. pmc beta-dystrobrevin, a member of the dystrophin-related protein family
    D J Blake
    Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 95:241-6. 1998
  9. pmc Reinvestigation of the dysbindin subunit of BLOC-1 (biogenesis of lysosome-related organelles complex-1) as a dystrobrevin-binding protein
    Ramin Nazarian
    Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Biochem J 395:587-98. 2006
  10. ncbi Identification and characterization of a novel member of the dystrobrevin gene family
    A A Puca
    Telethon Institute of Genetics and Medicine TIGEM, Milan, Italy
    FEBS Lett 425:7-13. 1998

Research Grants

Detail Information

Publications220 found, 100 shown here

  1. ncbi Role of -SH groups in rat sugar intestinal transport in vivo
    N Diez
    Departamento de Fisiología y Nutrición, Universidad de Navarra, Pamplona, Spain
    Rev Esp Fisiol 48:127-32. 1992
    ..of p-chloromercuribenzoic acid (pCMB), either alone or in the presence of 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), on the 1 mM galactose absorption by in vivo perfused rat intestine has been studied. At 0...
  2. pmc Cloning and expression of the gene encoding the Thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and biochemical characterization of the enzyme
    D S Burdette
    Biochemistry Department, Michigan State University, East Lansing 48824, USA
    Biochem J 316:115-22. 1996
    ..The enzyme was inactivated by chemical modification with dithionitrobenzoate (DTNB) and diethylpyrocarbonate, indicating that Cys and His residues are involved in catalysis...
  3. ncbi Polyamine and redox modulation of [3H]MK-801 binding to N-methyl-D-aspartate receptors in the spinal cord and cerebral cortex
    E Sundstrom
    Division of Geriatric Medicine, Neurotec, Karolinska Institutet, Stockholm, Sweden
    Pharmacol Toxicol 88:342-8. 2001
    ..2 mM 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) in the cerebral cortex while there was only a partial effect in the spinal cord...
  4. ncbi Interleukin-8, nitric oxide and glutathione status in proliferative vitreoretinopathy and proliferative diabetic retinopathy
    Erdogan Cicik
    Department of Ophthalmology, Istanbul University, Cerrahpasa Medical Faculty, Istanbul, Turkey
    Ophthalmic Res 35:251-5. 2003
    ....
  5. ncbi Potentiation of acid-sensing ion channels by sulfhydryl compounds
    Jun Hyeong Cho
    Dept of Neuroscience, The Ohio State University, Columbus, OH 43210, USA
    Am J Physiol Cell Physiol 292:C2161-74. 2007
    ..This effect was reversed by the oxidizing agent DTNB in hASIC1a, supporting the hypothesis that DTT also impacts ASICs via a redox-sensitive site...
  6. doi MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation
    Angela Lanciotti
    Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Neurobiol Dis 37:581-95. 2010
    ..Because pathological mutations prevent MLC1 membrane expression, the identification of substances regulating MLC1 intracellular trafficking is potentially relevant for the therapy of MLC...
  7. ncbi beta-dystrobrevin, a new member of the dystrophin family. Identification, cloning, and protein associations
    M F Peters
    Department of Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7545, USA
    J Biol Chem 272:31561-9. 1997
    ....
  8. pmc beta-dystrobrevin, a member of the dystrophin-related protein family
    D J Blake
    Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 95:241-6. 1998
    ..This finding may be relevant to the cognitive dysfunction affecting many patients with Duchenne muscular dystrophy...
  9. pmc Reinvestigation of the dysbindin subunit of BLOC-1 (biogenesis of lysosome-related organelles complex-1) as a dystrobrevin-binding protein
    Ramin Nazarian
    Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Biochem J 395:587-98. 2006
    ..Taken together, these results suggest that dysbindin assembled into BLOC-1 is not a physiological binding partner of the dystrobrevins, likely due to engagement of its dystrobrevin-binding region in interactions with other subunits...
  10. ncbi Identification and characterization of a novel member of the dystrobrevin gene family
    A A Puca
    Telethon Institute of Genetics and Medicine TIGEM, Milan, Italy
    FEBS Lett 425:7-13. 1998
    ..Immunostaining studies indicate that DTN-B and DTN expression is absent in affected muscle fibers from DMD patients and carriers...
  11. pmc No selenium required: reactions catalyzed by mammalian thioredoxin reductase that are independent of a selenocysteine residue
    Adam P Lothrop
    Department of Biochemistry, University of Vermont, 89 Beaumont Avenue, Given Building Room B413, Burlington, Vermont 05405, USA
    Biochemistry 48:6213-23. 2009
    ..Tat protein, H(2)O(2), lipid hydroperoxides, vitamin K, ubiquinone, juglone, ninhydrin, alloxan, dehydroascorbate, DTNB, lipoic acid/lipoamide, S-nitrosoglutathione, selenodiglutathione, selenite, methylseleninate, and selenocystine...
  12. doi Advantages of the WRAIR whole blood cholinesterase assay: comparative analysis to the micro-Ellman, Test-mate ChE, and Michel (DeltapH) assays
    Julian R Haigh
    Walter Reed Army Institute of Research, Division of Biochemistry, Silver Spring, MD 20910 7500, USA
    Chem Biol Interact 175:417-20. 2008
    ..These samples were measured using four different ChE assays: (i) colorimetric microEllman (using DTNB at 412 nm), (ii) Test-mate ChE field kit (also based on the Ellman assay), (iii) Michel (delta pH), and (iv) the ..
  13. pmc Flavodoxin:quinone reductase (FqrB): a redox partner of pyruvate:ferredoxin oxidoreductase that reversibly couples pyruvate oxidation to NADPH production in Helicobacter pylori and Campylobacter jejuni
    Martin St Maurice
    Department of Medicine, Division of Infectious Diseases, University of Virginia Health Systems, 409 Lane Road, Charlottesville, VA 22908, USA
    J Bacteriol 189:4764-73. 2007
    ..or benzoquinone and weak activity with cytochrome c, molecular oxygen, and 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB)...
  14. doi Assessment of acetylcholinesterase activity in Clarias gariepinus as a biomarker of organophosphate and carbamate exposure
    Robinson H Mdegela
    Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro, Tanzania
    Ecotoxicology 19:855-63. 2010
    ..and brain homogenates of unexposed and exposed fish using Ellman's method and 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) chromophore...
  15. pmc DNA damage, apoptosis and cell cycle changes induced by fluoride in rat oral mucosal cells and hepatocytes
    Ling Fei He
    Department of Dental Medicine, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei Province, China
    World J Gastroenterol 12:1144-8. 2006
    ..To study the effect of fluoride on oxidative stress, DNA damage and apoptosis as well as cell cycle of rat oral mucosal cells and hepatocytes...
  16. pmc Thiol/disulfide exchange is required for membrane fusion directed by the Newcastle disease virus fusion protein
    Surbhi Jain
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    J Virol 81:2328-39. 2007
    ..We found that inhibitors of cell surface thiol/disulfide isomerase activity--5'5-dithio-bis(2-nitrobenzoic acid) (DTNB), bacitracin, and anti-protein disulfide isomerase antibody--inhibited cell-cell fusion and virus entry but had no ..
  17. ncbi Modulation of [3H]dopamine release by glutathione in mouse striatal slices
    Réka Janáky
    Brain Research Center, University of Tampere Medical School, Tampere, Finland
    Neurochem Res 32:1357-64. 2007
    ..5,5'-Dithio-bis-2-nitrobenzoate (DTNB) and L-cystine were also inhibitory, while dithiothreitol (DTT) and L-cysteine enhanced the K(+)-evoked release...
  18. ncbi Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin
    Emadeldin H E Konozy
    Departamento de Biologia Celular, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, 14049 900 Ribeirao Preto, Brazil
    Biochimie 84:1035-43. 2002
    ..Chemical modification studies of both EiLL and Erythrina indica seed lectin (EiSL) with phenylglyoxal, DEP and DTNB revealed an absence of arginine, histidine and cysteine, respectively, in or near the ligand-binding site of both ..
  19. ncbi Essential cysteines in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from Escherichia coli: analysis by chemical modification and site-directed mutagenesis
    H M Salleh
    Interdepartmental Program in Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109 1065, USA
    Biochemistry 35:8942-7. 1996
    ..8-P) and inorganic phosphate (Pi) was inactivated by the thiol-modifying reagents 5,5-dithiobis (2-nitrobenzoate) (DTNB) and methyl methanethiosulfonate (MMTS)...
  20. ncbi Redox modulation of peripheral T-type Ca2+ channels in vivo: alteration of nerve injury-induced thermal hyperalgesia
    Slobodan M Todorovic
    Department of Anesthesiology, University of Virginia Health System, Mail Box 800710, Charlottesville, VA 22908 0710, USA
    Pain 109:328-39. 2004
    ..Here, we studied the effects of locally applied redox agents (L-cysteine and 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) on thermal hyperalgesia in animals with neuropathic pain due to chronic constrictive injury (CCI) of the sciatic ..
  21. ncbi Role of cysteine 337 and cysteine 340 in flavoprotein that functions as NADH oxidase from Amphibacillus xylanus studied by site-directed mutagenesis
    K Ohnishi
    Department of Agricultural Chemistry, Tokyo University of Agriculture, Japan
    J Biol Chem 270:5812-7. 1995
    ..The NADH:5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) oxidoreductase activities of all mutant enzymes were less than 3% of the activity of wild-type enzyme...
  22. ncbi H(2)O(2) modulates purinergic-dependent calcium signalling in osteoblast-like cells
    Paola D'Andrea
    Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Universita degli Studi di Trieste, Trieste, Italy
    Cell Calcium 43:457-68. 2008
    ..On the other hand, the membrane-impermeant thiol oxidant DTNB induced calcium oscillations that were inhibited by incubating the cells with suramin or apyrase...
  23. ncbi Chemical modification of specific active site amino acid residues of Enterobacter aerogenes glycerol dehydrogenase
    Anjali Pandey
    Department of Chemistry, Indian Institute of Technology, Kanpur
    J Enzyme Inhib Med Chem 17:49-53. 2002
    ..b>DTNB titration showed the presence of only a single cysteine residue per monomer of G1DH...
  24. ncbi Acetyl-CoA hydrolase activity and function in Ascaris suum muscle mitochondria
    Z S de Mata
    Department of Biological Sciences, University of Notre Dame, IN 46556, USA
    Comp Biochem Physiol B Biochem Mol Biol 116:379-83. 1997
    ..However, when incubated in the presence of 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), acetyl-CoA or propionyl-CoA are hydrolyzed to form free coenzyme A...
  25. pmc Role of beta-dystrobrevin in nonmuscle dystrophin-associated protein complex-like complexes in kidney and liver
    N Y Loh
    Department of Human Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, United Kingdom
    Mol Cell Biol 21:7442-8. 2001
    ..the role of beta-dystrobrevin in the function of nonmuscle tissues, we generated beta-dystrobrevin-deficient (dtnb(-/-)) mice by gene targeting. dtnb(-/-) mice are healthy, fertile, and normal in appearance...
  26. doi Differential cold-adaptation among protein components of the thioredoxin system in the psychrophilic eubacterium Pseudoalteromonas haloplanktis TAC 125
    Roberta Cotugno
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, Via S Pansini 5, 80131, Napoli, Italy
    Mol Biosyst 5:519-28. 2009
    ..In particular, rPhTrxR catalyses the NADPH dependent reduction of DTNB and rPhTrx provokes the insulin precipitation in the presence of DTT...
  27. pmc Dystrobrevin and dystrophin family gene expression in zebrafish
    Sabrina Böhm
    Department of Medical and Molecular Genetics, Guy s Campus, King s College London, London SE1 9RT, UK
    Gene Expr Patterns 8:71-8. 2008
    ..beta-dystrobrevin (dtnb) is expressed in the pronephric duct and widely at low levels...
  28. ncbi The sulfonylurea-inhibited NADH oxidase activity of HeLa cell plasma membranes has properties of a protein disulfide-thiol oxidoreductase with protein disulfide-thiol interchange activity
    D J Morre
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA
    J Bioenerg Biomembr 30:477-87. 1998
    ..Direct assay of protein thiols by reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB; Ellman's reagent), suggests that protein disulfides may be the natural electron acceptors for NADH oxidation by ..
  29. ncbi Detection of glutathione reductase after electrophoresis on native or sodium dodecyl sulfate polyacrylamide gels
    Wen Chi Hou
    Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan
    Electrophoresis 25:2926-31. 2004
    ..9) containing 4.0 mM oxidized glutathione (GSSG), 1.5 mM NADPH, and 2 mM 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) for 20 min. The GR activity was negatively stained in the dark by a solution containing 1...
  30. ncbi Comparison of the conformational stability of the non-native alpha-helical intermediate of thiol-modified beta-lactoglobulin upon interaction with sodium n-alkyl sulfates at two different pH
    J Chamani
    Department of Biology, Faculty of Science, Islamic Azad University Mashhad Branch, Mashhad, Iran
    J Colloid Interface Sci 299:636-46. 2006
    ..This thiol group was specifically reacted with DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) at pH 7...
  31. ncbi Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    J Vet Med Sci 69:627-32. 2007
    ..GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3...
  32. doi Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages
    Celio X C Santos
    Vascular Biology Laboratory, Heart Institute InCor, University of Sao Paulo School of Medicine, CEP 05403 000 São Paulo, Brazil
    J Leukoc Biol 86:989-98. 2009
    ..but not amastigote phagocytosis was inhibited significantly by macrophage incubation with thiol/PDI inhibitors DTNB, bacitracin, phenylarsine oxide, and neutralizing PDI antibody in a parasite redox-dependent way...
  33. ncbi Chemical modification of beta-glucosidase from Trichoderma reesei QM 9414
    I de la Mata
    Departamento de Bioquimica y Biologia Molecular I, Facultad de Ciencias Quimicas, Universidad Complutense de Madrid, Spain
    J Biochem 114:754-9. 1993
    ..002 mM-1.min-1. Treatment of the modified enzyme with 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) showed that one cysteine residue was essential for activity. At pH 5...
  34. ncbi Depolarization of isolated brain nerve endings by nitric oxide donors: membrane mechanisms
    A A Mongin
    Albany Medical College A 60, Albany, NY, 12208, USA
    Biochemistry (Mosc) 63:662-70. 1998
    ..The effects of NO donors were mimicked by SH-modifying reagents including 5, 5'-dithio-bis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM)...
  35. ncbi [Differences of acetylcholinesterase level in variety classes and strains of Culex pipiens pallens]
    Shi Gen Li
    Department of Parasitology, Jining Medical College, Jining 272013, China
    Zhejiang Da Xue Xue Bao Yi Xue Ban 38:511-4. 2009
    ..To investigate the diversity of acetylcholinesterase (AChE) activity in variety classes and strains of Culex pipiens pallens and provide a basis for the insecticide-resistance detection of mosquito by biochemical method...
  36. ncbi Dibucaine inhibition of serum cholinesterase
    Babiker Elamin
    Department of Biochemistry, College of Science, King Saud University, P O Box 2455, Riyadh 11451, Saudi Arabia
    J Biochem Mol Biol 36:149-53. 2003
    ..The enzyme was assayed colorimetrically by the reaction of 5,5'-dithiobis-[2-nitrobenzoic acid] (DTNB) with the free sulfhydryl groups of thiocholine that were produced by the enzyme reaction with butrylthiocholine (..
  37. ncbi Purification and catalytic properties of glutathione transferase from the hepatopancreas of crayfish macrobrachium vollenhovenii (herklots)
    Isaac Olusanjo Adewale
    Department of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeria
    J Biochem Mol Toxicol 18:332-44. 2004
    ..Chemical modification studies with DTNB revealed that two sulphydryl groups per dimer were essential to the activity of the enzymes...
  38. pmc Uptake and reduction of alpha-lipoic acid by human erythrocytes
    James M May
    Department of Medicine, Vanderbilt University School of Medicine, 715 Preston Research Building, Vanderbilt University School of Medicine, Nashville, TN 37232 6303, USA
    Clin Biochem 40:1135-42. 2007
    ..In this work we targeted the antioxidant capacity of pyridine nucleotide disulfide reductases of these cells by measuring their ability to reduce the disulfide alpha-lipoic acid...
  39. doi Allicin and disulfiram enhance platelet integrin alphaIIbbeta3-fibrinogen binding
    Yoav Manaster
    Goldschleger Eye Research Institute and Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Israel
    Thromb Res 124:477-82. 2009
    ..The present study is aimed to support the hypothesis that prevention of specific alphaIIbbeta3 intra-molecular disulfide bond formation increases receptor-ligand binding activity...
  40. doi Sinapinic acid can replace ascorbate in the biotin switch assay
    Vasantha Madhuri Kallakunta
    Department of Chemistry and Biochemistry, 275 1, EH, University of Windsor, 401 Sunset Avenue, Windsor, Ontario N9B3P4, Canada
    Biochim Biophys Acta 1800:23-30. 2010
    ..We report a modification of the biotin switch assay where sinapinic acid is utilized instead of ascorbate to eliminate potential artifacts in the detection of S-nitrosated proteins...
  41. doi Evaluation of flow injection analysis for determination of cholinesterase activities in biological material
    Jiri Cabal
    Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
    Chem Biol Interact 187:225-8. 2010
    ..flowing to dialyzer; reaction product (thiocholine) is dialyzed and mixed with 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) transported to flow spectrophotometer. Flowing of all materials is realised using peristaltic pump...
  42. ncbi Purification and some properties of a chloramphenicol acetyltransferase mediated by plasmids from Vibrio anguillarum
    S Masuyoshi
    Laboratory of Drug Resistance in Bacteria, Gunma University School of Medicine, Japan
    J Biochem 104:131-5. 1988
    ..Enzyme activity was inhibited by HgCl2, p-chloromercuribenzoate (p-CMB), 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), and ethylendiaminotetraacetic acid (EDTA). The half life at 53 degrees C was approximately 100 min.
  43. ncbi Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes
    G Gao
    Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
    J Biol Chem 269:8234-9. 1994
    ..Incorporation of either 1 mol of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), 0.6 mol of iodo[3H]acetate, or 0...
  44. ncbi Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli
    M Liu
    State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Academia Sinica, China
    Biochemistry 38:11006-11. 1999
    ..Titration of sulfhydryl groups in ArgRS by 5, 5'-dithiobis(2-nitro benzoic acid) (DTNB) suggested that a disulfide bond was not formed in the enzyme and that, in the native condition, two DTNB-sensitive ..
  45. ncbi Hydrogen sulfide as a novel nociceptive messenger
    Atsufumi Kawabata
    Division of Physiology and Pathophysiology, Department of Pharmacy, School of Pharmacy, Kinki University, Higashi Osaka 577 8502, Japan
    Pain 132:74-81. 2007
    ..The H(2)S-evoked hyperalgesia was blocked by 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB), an oxidizing agent, or ethosuximide and mibefradil, T-type Ca(2+) channel inhibitors...
  46. ncbi Mono- (Ag, Hg) and di- (Cu, Hg) valent metal ions effects on the activity of jack bean urease. Probing the modes of metal binding to the enzyme
    Barbara Krajewska
    Jagiellonian University, Faculty of Chemistry, 30 060, Krakow, Ingardena 3, Poland
    J Enzyme Inhib Med Chem 23:535-42. 2008
    ..The titration carried out with DTNB was done to examine the involvement of urease thiol groups in metal ion binding...
  47. pmc Molecular docking studies of dithionitrobenzoic acid and its related compounds to protein disulfide isomerase: computational screening of inhibitors to HIV-1 entry
    Uthaman Gowthaman
    Bioinformatics Centres of BTISnet at Pondicherry University and IIT Delhi, India
    BMC Bioinformatics 9:S14. 2008
    ..This study attempts to perceive the mode of binding of dithionitrobenzoic acid (DTNB), and its structurally related compounds on PDI enzyme.
  48. doi Purification and characterization of a thiol amylase over produced by a non-cereal non-leguminous plant, Tinospora cordifolia
    Abhishek Mukherjee
    Department of Biotechnology, Heritage Institute of Technology, Anandapur, East Kolkata Township, Kolkata 700 107, West Bengal, India
    Carbohydr Res 345:2731-5. 2010
    ..Activity was stable in the pH range of 4-7 and at temperatures up to 60°C. PCMB, iodoacetic acid, iodoacetamide, DTNB, and heavy metal ions Hg(2+)>Ag(+)>Cd(2+) inhibited enzyme activity while Ca(2+) improved both activity and ..
  49. ncbi Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli
    M Yamaguchi
    J Biochem 86:325-33. 1979
    ..Similarly, other sulfhydryl reagents such as p-hydroxymercuribenzoate (PMB), 5,5'-dithiobis(2-nitrobenzoate) (DTNB), and iodoacetate inhibited the decarboxylase activities and activated the reductase activities to various extents...
  50. ncbi Low- and high-Km transport of dinitrophenyl glutathione in inside out vesicles from human erythrocytes
    T P Akerboom
    Institut fur Physiologische Chemie I, Universitat Dusseldorf, Germany
    Biochim Biophys Acta 1103:115-9. 1992
    ..inhibited by fluoride, vanadate, p-chloromercuribenzoate (pCMB) and bis(4-nitrophenyl)dithio-3,3'-dicarboxylate (DTNB). The low-Km uptake process was less sensitive to the inhibitory action of DTNB as compared to the high-Km process...
  51. ncbi The widespread distribution of alpha-N-trimethylalanine as the N-terminal amino acid of light chains from vertebrate striated muscle myosins
    G D Henry
    Eur J Biochem 148:75-82. 1985
    ..in all vertebrate striated muscle light chains of the A1-type (LC1, 'essential' light chains) and LC2-type ('DTNB'-light chains, 'phosphorylatable' light chains) but was absent from all invertebrate muscle and vertebrate smooth ..
  52. ncbi HPLC-based method using sample pre-column clean-up for the determination of methanethiol and ethanethiol in parenteral amino acid solutions
    P C do Nascimento
    Department of Chemistry, Federal University of Santa Maria, Brazil
    Fresenius J Anal Chem 370:92-6. 2001
    ..Simultaneous derivatization and elution with DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) enabled further discrimination between MT and ET by reversed-phase HPLC with ..
  53. ncbi Redox-based control of the gamma heavy chain ATPase from Chlamydomonas outer arm dynein
    Alistair Harrison
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    Cell Motil Cytoskeleton 52:131-43. 2002
    ..Mutational and biochemical dissection of the outer arm revealed that ATPase activation in response to DTNB was an exclusive property of the gamma heavy chain, and that enzymatic enhancement was modulated by the presence of ..
  54. ncbi Isolation and properties of cytoplasmic alpha-glycerol 3-phosphate dehydrogenase from the pectoral muscle of the fruit bat, Eidolon helvum
    Femi Kayode Agboola
    Department of Biochemistry, Obafemi Awolowo University, Ile Ife, Nigeria
    J Biochem Mol Biol 36:159-66. 2003
    ..9 +/- 0.2 x 10(4)s(-1). The treatment of the enzyme with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) under denaturing conditions indicated that there were a total of eight cysteine residues, while only two of these ..
  55. ncbi Soluble human core 2 beta6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity
    Xiaojing Yang
    Department of Medicine, Human Mobility Research Center, Queen s University, Kingston, Ontario, Canada
    Biochim Biophys Acta 1648:62-74. 2003
    ..Sulfhydryl reagents such as 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and N-ethylmaleimide (NEM) inactivated the enzyme, and the inactivation was partially prevented by prior addition ..
  56. ncbi [Effect of sodium nitroprusside on Na+, K+-ATPase of rat myocardium and renal cortex]
    O V Akopova
    Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv
    Ukr Biokhim Zh 75:54-9. 2003
    ..The Na-pump activation as well as the increase in the number of DTNB-reactive thiol groups was abolished after the detergent treatment (0...
  57. ncbi Purification, tandem mass characterization, and inhibition studies of oxidosqualene-lanosterol cyclase enzyme from bovine liver
    Tung Kung Wu
    Department of Biological Science and Technology, National Chiao Tung University, 300 Hsin Chu, Taiwan, China
    Arch Biochem Biophys 421:42-53. 2004
    ..79 microM, respectively. Results from DTNB modification and DTNB coupled with Ro48-8071 competition study suggest that two sulfhydryl groups are involved in ..
  58. ncbi [Malondialdehyde and free thiol groups from smokers' plasma]
    Cornelia Mircea
    Disciplina de Toxicologie, Facultatea de Farmacie, Universitatea de Mecină şi Famacie Gr T Popa Iaşi
    Rev Med Chir Soc Med Nat Iasi 107:184-7. 2003
    ..The determinations was effectuated with 2-thiobarbituric acid for malondialdehyde and with 5,5'-DTNB for thiol groups. The results was expressed in mmol/L for malondialdehyde and mmol/ml for free thiol groups...
  59. ncbi Inhibition of 5-amino levulinic acid dehydratase activity by arsenic in excised etiolated maize leaf segments during greening
    Meeta Jain
    School of Biochemistry, Devi Ahilya University, Takshashila Campus, Khandwa Road, Indore 452 017, India
    J Plant Physiol 161:251-5. 2004
    ..The % inhibition of enzyme activity was also reduced by in vivo supply of DTNB. The enzyme activity was reduced substantially by in vitro inclusion of LA, both in the absence and presence of As...
  60. ncbi Multi-step analysis of Hg2+ ion inhibition of jack bean urease
    Barbara Krajewska
    Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30 060 Krakow, Poland
    J Inorg Biochem 98:1160-8. 2004
    ..of thiol groups in urease-Hg(2+) complexes with a cysteine selective agent 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)...
  61. ncbi Cysteine-321 of human brain GABA transaminase is involved in intersubunit cross-linking
    Chang Sik Yoon
    Department of Genetic Engineering and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon 200 702, Korea
    Mol Cells 18:214-9. 2004
    ..Reaction of 1.5 sulfhydryl groups per wild type dimer with 5,5 cent-dithiobis-2-nitrobenzoic acid (DTNB) produced about 95% loss of activity. No reactive -SH groups were detected in the mutant enzymes...
  62. ncbi Acid sensing ionic channels: modulation by redox reagents
    Fedorenko Andrey
    Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev, Ukraine
    Biochim Biophys Acta 1745:1-6. 2005
    ..potentiates proton-activated currents, while the oxidizing reagent 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) causes their inhibition...
  63. ncbi Distinct response to ionizing radiation of human prostate cell lines
    Venera Cardile
    Department of Physiological Sciences, University of Catania, V le A Doria 6, I 95125 Catania, Italy
    Oncol Rep 14:981-5. 2005
    ..diacetate bis acetomethyl ester (DCFH-DA), GSH levels by 2,2'-dinitro-5,5'-dithio-benzoic acid (DTNB), and lipoperoxidation by thiobarbituric acid reactive substances (TBARS) analysis...
  64. ncbi Mercury(II) ion-selective polymeric membrane sensors for analysis of mercury in hazardous wastes
    Saad S M Hassan
    Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt
    Anal Sci 22:877-81. 2006
    ..These are based on the use of 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) and tricyclazole (TCZ) as neutral carriers in plasticized poly(vinyl chloride) membranes...
  65. ncbi Calcium-dependent conformational changes in guanylate cyclase-activating protein 2 monitored by cysteine accessibility
    Andreas Helten
    Biochemistry Group, IBU, Faculty V, University of Oldenburg, D 26111 Oldenburg, Germany
    Biochem Biophys Res Commun 356:687-92. 2007
    ..in wild type and mutant GCAP2 forms with the thiol-modifying reagent 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)...
  66. pmc Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1
    Yoshiyuki Ishii
    Center for Pathogen Genomics, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8640, Japan
    Virol J 4:110. 2007
    ..Although the authentic virions cannot be studied with cultured cells, surrogate pseudovirions consisting of capsid and reporter plasmid are available for studies dealing with infectivity...
  67. doi Increased chemosensitivity and elevated reactive oxygen species are mediated by glutathione reduction in glutamine deprived neuroblastoma cells
    Sakurako Izaki
    Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama City, 236 0004, Japan
    J Cancer Res Clin Oncol 134:761-8. 2008
    ..To evaluate the effects of glutamine concentrations, cell growth, GSH levels, oxidative stress, and chemosensitivity were evaluated in neuroblastoma cell lines...
  68. doi Investigation of the interactions of polyhedral borane anions with serum albumins
    William Jefferson McVey
    Department of Chemistry and Biochemistry, Texas State University, San Marcos, 601 University Drive, San Marcos, TX 78666, USA
    J Inorg Biochem 102:943-51. 2008
    ..for disulfide bond formation was confirmed by examining the reactions of the [B(20)H(17)SH](4-) ion with both DTNB and reduced glutathione...
  69. doi Determination of total and oxidized glutathione in human whole blood with a sequential injection analysis system
    André R T S Araujo
    REQUIMTE, Departamento de Quimica Fisica, Faculdade de Farmacia da Universidade do Porto, Rua Anibal Cunha, 164, 4099 030 Porto, Portugal
    Talanta 74:1511-9. 2008
    ..The determination was based on the DTNB-GSSG reductase recycling assay, which couples the specificity of the GSSG reductase (GR) with an amplification of ..
  70. pmc Inactivation of an invertebrate acetylcholinesterase by sulfhydryl reagents: a reconsideration of the implications for insecticide design
    M Rowland
    Department of Biology, Birmingham Southern College, Birmingham, AL 35254, USA
    Chem Biol Interact 175:73-5. 2008
    ..cholinesterase 2 from amphioxus, by the sulfhydryl reagents 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM)...
  71. doi Redox modulation at the peripheral site alters nociceptive transmission in vivo
    Flavia C Meotti
    Department of Physiological Science, Federal University of Santa Catarina, Florianopolis, Brazil
    Clin Exp Pharmacol Physiol 36:272-7. 2009
    ..groups in footpad tissue were quantified using a colourimetric reaction with 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB). 2...
  72. doi Protective role of ortho-substituted Mn(III) N-alkylpyridylporphyrins against the oxidative injury induced by tert-butylhydroperoxide
    Ana S Fernandes
    iMed UL, Faculty of Pharmacy, University of Lisbon, Lisboa, Portugal
    Free Radic Res 44:430-40. 2010
    ..MTT and crystal violet assays), intracellular O(2)*- generation (dihydroethidium assay) and glutathione status (DTNB and monochlorobimane assays). MnPs per se did not show cytotoxicity (up to 25 microM, 24 h)...
  73. doi Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysis
    Sandeep Kumar
    School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India
    Plant Physiol Biochem 48:746-50. 2010
    ..The native urease incubated with 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) showed exponential increase in the absorbance, thereby revealing the presence of -SH groups...
  74. doi Characterization of Deinococcus radiophilus thioredoxin reductase active with both NADH and NADPH
    Hee Jeong Seo
    Department of Microbiology, Chungbuk National University, Cheongju 361 763, Republic of Korea
    J Microbiol 48:637-43. 2010
    ..2 μM and 192 μM/min. The Km and Vmax for 5, 5'-dithio-bis-2-nitrobenzoic acid (DTNB, a substituted substrate for thioredoxin) are 463 μM and 756 μM/min, respectively...
  75. doi Site-directed mutagenesis of mouse glutathione transferase P1-1 unlocks masked cooperativity, introduces a novel mechanism for 'ping pong' kinetic behaviour, and provides further structural evidence for participation of a water molecule in proton abstract
    Gavin McManus
    School of Biochemistry and Immunology, Trinity College, Dublin, Ireland
    FEBS J 278:273-81. 2011
    ..6. Only a small proportion of C14 is reactive with 5,5'-dithiobis-(2-nitrobenoic acid) (DTNB) at pH 9 in the C47A/C169A double mutant...
  76. ncbi Identification and conformer analysis of a novel redox-active motif, Pro-Ala-Ser-Cys-Cys-Ser, in Drosophila thioredoxin reductase by semiempirical molecular orbital calculation
    Mitsuhiko Kuwahara
    Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
    Biosci Biotechnol Biochem 75:516-21. 2011
    ..4/min for DTNB and 13.2/min for E. coli trx) compared to the Sec-to-Cys mutant...
  77. pmc Atomic level characterization of the nonproton ligand-sensing domain of ASIC3 channels
    Ye Yu
    Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    J Biol Chem 286:24996-5006. 2011
    ..in coordinating GMQ responses are also critical for activation of the ASIC3(E79C) mutant by thiol-reactive compound DTNB. Thus, a range of complementary techniques provide independent evidence for the structural details of the GMQ-..
  78. doi Quantitative measurements of glutathione in yeast cell lysate using 1H NMR
    Steve Y Rhieu
    Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
    Anal Bioanal Chem 405:4963-8. 2013
    ..The level of GSH measured by (1)H NMR was in good agreement with the concentration measured by the DTNB-GSSG reductase recycling assay...
  79. doi Hydrogen peroxide-induced reduction of delayed rectifier potassium current in hippocampal neurons involves oxidation of sulfhydryl groups
    Sonia M K Hasan
    Department of Physiology, Faculty of Medicine, Kuwait University, P O Box 24923, Safat 13110, Kuwait
    Brain Res 1520:61-9. 2013
    ..Application of the sulfhydryl-oxidizing agent 5,5 dithio-bis-nitrobenzoic acid (DTNB) mimicked the effect of H2O2...
  80. pmc NrdH Redoxin enhances resistance to multiple oxidative stresses by acting as a peroxidase cofactor in Corynebacterium glutamicum
    Mei Ru Si
    State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A and F University, Yangling, Shaanxi, People s Republic of China
    Appl Environ Microbiol 80:1750-62. 2014
    ..the disulfides in the class Ib ribonucleotide reductases (RNR), insulin and 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), by exclusively receiving electrons from thioredoxin reductase...
  81. doi Influence of analgesic active 3-[4-(3-trifluoromethyl-phenyl)-piperazin-1-yl]-dihydrofuran-2-one on the antioxidant status, glucose utilization and lipid accumulation in some in vitro and ex vivo assays
    Kinga Sałat
    Department of Pharmacodynamics
    Toxicol Mech Methods 24:204-11. 2014
    ....
  82. pmc Micro-method for the determination of glutathione in human blood
    Daniela Giustarini
    Department of Life Sciences, Laboratory of Pharmacology and Toxicology, University of Siena, Via A Moro 4, 53100 Siena, Italy Electronic address
    J Chromatogr B Analyt Technol Biomed Life Sci 964:191-4. 2014
    ..GSH is then measured in the clear supernatant by colorimetry using DTNB, i.e., 5,5'-dithio-bis(2-nitrobenzoic acid), in aqueous solution at pH 7.8...
  83. doi Inactivation of sortase A mediated by metal ATCUN complexes
    Insiya Fidai
    Evans Laboratory of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH, 43210, USA
    J Biol Inorg Chem 19:1327-39. 2014
    ..Results obtained from a DTNB assay support oxidation of the Cys-184 residue. LC-MS/MS also suggested oxidation of the Arg-197 containing peptide...
  84. doi Kinetic and thermodynamic studies on the disulfide-bond reducing potential of hydrogen sulfide
    Anita Vasas
    Department of Molecular Immunology and Toxicology, National Institute of Oncology, Ráth György utca 7 9, Budapest 1122, Hungary Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1, Debrecen 4010, Hungary
    Nitric Oxide 46:93-101. 2015
    ..Using DTNB (5,5'-dithiobis-(2-nitrobenzoic acid), also known as Ellman's reagent) as a model disulfide we conducted a ..
  85. pmc Poststroke depression as a factor adversely affecting the level of oxidative damage to plasma proteins during a brain stroke
    Natalia Cichoń
    Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141 143, 90 236 Lodz, Poland
    Oxid Med Cell Longev 2015:408745. 2015
    ..Additionally, we checked differences in proteins thiol groups by spectrophotometric assay based on reaction between DTNB and thiols. We also evaluated catalase activity in erythrocytes measured as ability to decompose H2O2...
  86. ncbi Structural properties of cyanase. Denaturation, renaturation, and role of sulfhydryls and oligomeric structure in catalytic activity
    R M Little
    J Biol Chem 262:10120-6. 1987
    ..The one sulfhydryl group per subunit does not react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) unless cyanase is denatured...
  87. ncbi Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer
    Myun K Han
    Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007, USA
    Biochemistry 41:3468-76. 2002
    ..This protein contains two cysteine residues per monomer. Studies of reactions with DTNB show that both cysteines are reactive and exhibit biphasic reaction kinetics...
  88. ncbi Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimer
    Sergio Enriquez-Flores
    Laboratorio de Bioquímica Genética y Dirección de Investigación, Instituto Nacional de Pediatria, 04530 Mexico, DF, Mexico
    Mol Biochem Parasitol 157:179-86. 2008
    ..the dimer to stable monomers were reached when four Cys were derivatized by 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB). The effects of DTNB were completely abolished when GlTIM was first treated with MMTS...
  89. ncbi Interaction of substrates and inhibitors with the homoserine dehydrogenase of kinase-inactivated aspartokinase I
    J K Wright
    Biochemistry 16:1541-8. 1977
    ..7). Threonine still protected the SH groups of I against 5,5'-dithiobis(2-nitrobenzoate) (DTNB) reaction but the number of SH groups reacting with thiol reagents (DTNB) was reduced by 1-2 per subunit in the ..
  90. ncbi Effect of p-chloromercuribenzoate on Clostridium perfringens beta toxin
    J Sakurai
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan
    Toxicon 30:323-30. 1992
    ..Treatment of the toxin with N-ethylmaleimide (NEM), 5,5'-dithio-bis(2-nitro-benzoic acid) (DTNB), o-iodosobenzoate (OIBA) and metal ions such as Cu2+ and Ag+ decreased the lethal activity, but PCMB did not ..
  91. ncbi LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides
    W S Harris
    Department of Medicine, University of Kansas Medical Center, Kansas City 66103
    Lipids 25:341-3. 1990
    5,5-Dithiobis-(2-nitrobenzoic acid) (DTNB) and p-chloro-mercuriphenylsulfonic acid (PCMPS) are well-known sulfhydryl inhibitors that are used to inhibit lecithin: cholesterol acyltransferase (LCAT)...
  92. ncbi Specific binding of a novel compound, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) to the active site of cAMP-dependent protein kinase
    M Hagiwara
    Mol Pharmacol 31:523-8. 1987
    ..F3GA but did not afford any protection against the covalent modification of 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl), suggesting that the binding site of H-8 does not involve the ..
  93. ncbi A cysteine in the C-terminal region of alanyl-tRNA synthetase is important for aminoacylation activity
    M X Wu
    Department of Biochemistry, Loma Linda University School of Medicine, California 92350
    Biochemistry 33:12260-6. 1994
    ..residues with p-(hydroxymercuri)phenylsulfonic acid, monobromobimane, and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)...
  94. ncbi Electrospray mass spectrometry of alpha and beta chains of selected hemoglobins and their TNBA and TNB conjugates
    M Adamczyk
    Department of Chemistry D9NM, Abbott Laboratories, Abbott Park, Illinois 60064 USA
    Bioconjug Chem 8:400-6. 1997
    ..prepared for 14 of the globins using 2 traditional colorimetric derivatizing reagents, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and trinitrobenzenesulfonic acid (TNBS), and the mass of each conjugate was determined by mass spectrometry.
  95. ncbi Synthesis of 5,5'-dithiobis(2-nitrobenzamides) as alternative substrates for trypanothione reductase and thioredoxin reductase: a microtiter colorimetric assay for inhibitor screening
    E Davioud-Charvet
    Institut de Biologie de Lille Institut Pasteur de Lille, URA CNRS 1309, 1 rue du Professeur Calmette, Lille Cedex, 59021, France
    Anal Biochem 268:1-8. 1999
    ..As with 5, 5'-dithiobis(2-nitrobenzoate) (DTNB), which can be reduced by TRs and TrxRs, the new compounds are converted to their corresponding chromophoric ..
  96. ncbi Metabolism of oxidized phosphatidylcholines formed in oxidized low density lipoprotein by lecithin-cholesterol acyltransferase
    H Itabe
    Department of Microbiology and Molecular Pathology, School of Medicine, Fukuoka University, Fukuoka, 814 0180, Japan
    J Biochem 126:153-61. 1999
    ..The addition of dithio-bis(nitrobenzoate) (DTNB), an LCAT inhibitor, or the inactivation of LCAT activity by treating the plasma at 56 degrees C for 30 min ..
  97. ncbi Differential mechanisms of urethral smooth muscle relaxation by several NO donors and nitric oxide
    A Garcia-Pascual
    Departamento de Fisiologia, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
    Naunyn Schmiedebergs Arch Pharmacol 360:80-91. 1999
    ..Oxidation of sarcolemmal thiol groups with 5-5'-dithio-bis[2-nitrobenzoic acid] (DTNB; 0...
  98. ncbi Irreversible activation of cyclic nucleotide-gated ion channels by sulfhydryl-reactive derivatives of cyclic GMP
    R L Brown
    Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, Oregon 97209, USA
    Biochemistry 39:432-41. 2000
    ..of the patch with the sulfhydryl-blocking reagents N-ethylmaleimide (NEM) and bis-dithionitrobenzoic acid (DTNB) prevented covalent activation; the effect of DTNB was reversed by reduction with DTT...
  99. ncbi Redox modulation of T-type calcium channels in rat peripheral nociceptors
    S M Todorovic
    Department of Anesthesiology, Washington University School of Medicine, 63110, St Louis, MO, USA
    Neuron 31:75-85. 2001
    ..This hyperalgesia is blocked by the oxidizing agent 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the T channel antagonist mibefradil. DTNB alone and in combination with mibefradil induces thermal analgesia...
  100. ncbi Elevation of brain glutathione by gamma-glutamylcysteine ethyl ester protects against peroxynitrite-induced oxidative stress
    Jennifer Drake
    Department of Chemistry and Center of Membrane Sciences, University of Kentucky, Lexington, Kentucky, 40506, USA
    J Neurosci Res 68:776-84. 2002
    ..injection of GCEE to gerbils resulted in a 41% increase in brain total GSH levels in vivo as determined by the DTNB-GSH reductase recycling method...
  101. ncbi A general strategy for site-specific double labeling of globular proteins for kinetic FRET studies
    V Ratner
    Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
    Bioconjug Chem 13:1163-70. 2002
    ..of the reaction of each engineered cysteine residue with a reference reagent [5,5'-dithiobis(2-nitrobenzoic acid) (DTNB)] were determined...

Research Grants23

  1. MULTIPLE CLASSES OF CA2+ CHANNELS - SENSORY TRANSMISSION
    SLOBODAN TODOROVIC; Fiscal Year: 2004
    ..abstract_text> ..
  2. CONTRIBUTION OF COLLAGEN TO VENTRICULAR STIFFNESS
    JAMES CAULFIELD; Fiscal Year: 1990
    ..rat hearts in vitro or dog hearts in vivo with oxidized glutathione or 5, 5'- Dithio-bis (2 nitrobenzoic) acid (DTNB) to effect removal of tissue collagen...
  3. Antioxidant Interaction of Selenium and Vitamins C & E
    James May; Fiscal Year: 2008
    ..Using this approach, it will be possible to relate the crucial, mitochondrial functions of selenium and vitamins C and E to the antioxidant defenses of the whole animal. ..
  4. Antioxidant Functions of Lipoic Acid
    James May; Fiscal Year: 2004
    ..Despite the widespread use of lipoic acid, these studies are necessary to establish the biological basis and rationale for the clinical use of the agent. ..
  5. Antioxidant vitamins in models of Alzheimer's Disease
    James May; Fiscal Year: 2008
    ..If antioxidant vitamins ameliorate toxicity in cell and animal models of Alzheimer's disease, then oxidant stress is involved in disease progression, and antioxidant vitamin supplements may be beneficial. ..
  6. ApoA-I Helical Domains and Cardiovascular Aging
    JOHN BIELICKI; Fiscal Year: 2005
    ..These studies are relevant for devising novel strategies to combat cardiovascular aging. ..
  7. REDOX PHARMACOLOGY OF T CHANNELS IN DRG NEURONS
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..In addition, our results suggest an unexplored avenue for the development of potential novel therapies for pain control. ..
  8. Prenatal Virally Induced Brain Disorder in Mouse
    SEYYED FATEMI; Fiscal Year: 2009
    ..In addition, it is expected that the results will provide clues that will lead to fundamental advances in our knowledge of the pathogenesis of schizophrenia and, therefore, of how it can be prevented and treated. ..
  9. Varenicline and Smoking Cessation in Schizophrenia
    SEYYED HOSSEIN FATEMI; Fiscal Year: 2010
    ..Such outcomes will be significant, because they will offer a new treatment for smoking cessation in this vulnerable population. ..
  10. Methamphetamine Neurotoxicity and Microglial Activation
    DONALD KUHN; Fiscal Year: 2009
    ..A better understanding of the mechanisms by which METH causes persistent damage to the DA neuronal system could lead to more targeted therapies and would open newer lines of investigation into this and other neurotoxic drugs of abuse. ..
  11. Redox Regulation of Cardiac Electrical Remodeling
    GEORGE ROZANSKI; Fiscal Year: 2009
    ..PHS 398 (Rev. 09/04) Page 2 Principal Investigator/Program Director (Last, first, middle): Rozanski, George, J ..
  12. ABCA1 agonist peptides for therapeutic use
    JOHN BIELICKI; Fiscal Year: 2008
    ..The research is likely to have a high impact on public health, since the therapeutics will be amenable to the widespread treatment of atherosclerosis. [unreadable] [unreadable] [unreadable] [unreadable]..
  13. Molecular Neurobiology of Drug Abuse
    DONALD KUHN; Fiscal Year: 2006
    ..Kuhn will remain extensively engaged in scientific mentoring activities. ..
  14. Acetylcholinesterase: A Comparative Biochemical Approach
    Leo Pezzementi; Fiscal Year: 2005
    ..abstract_text> ..
  15. TSQ Quantum ESI-MS Surveyor Bundle
    David Ford; Fiscal Year: 2004
    ..This requested instrumentation will remedy this deficit in our otherwise well-equipped facility. ..
  16. PLASMALOGEN CATABOLISM DURING MYOCARDIAL ISCHEMIA
    David Ford; Fiscal Year: 2003
    ..Specific Aim 3 is to test the hypothesis that myocardial ischemia/reperfusion injury is mediated, in part, by plasmalogen degradation by reactive chlorinating species released from activated neutrophils. ..
  17. PKC SIGNALING AND THE TREATMENT OF BIPOLAR DISORDER
    DONALD KUHN; Fiscal Year: 2002
    ....
  18. PRESERVATION OF HDL FUNCTION DURING EARLY ATHEROGENESIS
    JOHN BIELICKI; Fiscal Year: 2002
    ..Moreover, the research will uncover novel mechanisms for preserving HDL/LCAT function that can be utilized therapeutically to fight the onset of cardiovascular disease. ..
  19. Calorimetry Work Station
    SHERWIN LEHRER; Fiscal Year: 2002
    ..iii) measure binding constants and characterize the thermodynamics of binding equilibria. ITC avoids the unavoidable perturbation of fluorescence labels and is more quantitatively than ELISA assays of binding. ..
  20. Anesthetics block neuronal voltage-gated Ca2+ currents
    SLOBODAN TODOROVIC; Fiscal Year: 2009
    ..abstract_text> ..
  21. TROPOMYOSIN AND THE REGULATION OF MUSCLE CONTRACTION
    SHERWIN LEHRER; Fiscal Year: 2009
    ..These measurements between specific labeled amino acid residues will provide information about the dynamics of the changes between the components involved in the regulatory process. ..
  22. NEUROSCIENCE TRAINING IN DRUG ABUSE
    DONALD KUHN; Fiscal Year: 2004
    ..abstract_text> ..
  23. MICROARRAY ANALYSIS OF HUMAN COCAINE ADDICTS
    DONALD KUHN; Fiscal Year: 2002
    ..Microarray-based scanning of the human genome has the potential to establish those genes or gene clusters that define the addictive phenotype, leading the way to proteomic-based studies of the bases of addiction. ..