Genomes and Genes
Gene Symbol: DPH1
Description: diphthamide biosynthesis 1
Alias: DEDSSH, DPH2L, DPH2L1, OVCA1, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1, DPH-like 1, DPH1 homolog, DPH2-like 1, S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1, candidate tumor suppressor in ovarian cancer 1, diphthamide biosynthesis protein 1, diphthamide biosynthesis protein 2 homolog-like 1, diphtheria toxin resistance protein 1, diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1, ovarian cancer-associated gene 1 protein, ovarian tumor suppressor candidate 1
- Loss of diphthamide pre-activates NF-κB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factorSebastian Stahl
Roche Pharma Research and Early Development, Large Molecule Research, Roche Innovation Center Penzberg, 82377 Penzberg, Germany
Proc Natl Acad Sci U S A 112:10732-7. 2015..This modification is synthesized by seven dipthamide biosynthesis proteins (DPH1-DPH7) and is conserved among eukaryotes and archaea...
- Dph3 is an electron donor for Dph1-Dph2 in the first step of eukaryotic diphthamide biosynthesisMin Dong
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States
J Am Chem Soc 136:1754-7. 2014..Previous genetic studies showed this step requires four proteins in eukaryotes, Dph1-Dph4. However, the exact molecular functions for the four proteins are unknown...
- Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesisZhewang Lin
Department of Chemistry and Chemical Biology and Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853, United States
J Am Chem Soc 136:6179-82. 2014..The biosynthesis of diphthamide was proposed to occur in three steps requiring seven proteins, Dph1-7, in eukaryotes. The functional assignments of Dph1-5 in the first and second step have been well established...
- Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker syndromeYi Ru Yu
Department of Life Sciences and Institute of Genome Sciences
Hum Mol Genet 23:5579-96. 2014b>OVCA1/DPH1 (OVCA1) encodes a component of the diphthamide biosynthesis pathway and is located on chromosome 17p13.3. Deletions in this region are associated with Miller-Dieker syndrome (MDS)...
- The diphthamide modification pathway from Saccharomyces cerevisiae--revisitedRaffael Schaffrath
Department of Genetics, University of Leicester, Leicester, LE1 7RH, UK Institut für Biologie, Abteilung Mikrobiologie, Universitat Kassel, 34132, Kassel, Germany
Mol Microbiol 94:1213-26. 2014..However, recent progress in dissecting the diphthamide gene network (DPH1-DPH7) from the budding yeast Saccharomyces cerevisiae has significantly advanced our understanding of the ..
- Accelerating novel candidate gene discovery in neurogenetic disorders via whole-exome sequencing of prescreened multiplex consanguineous familiesAnas M Alazami
Department of Genetics, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
Cell Rep 10:148-61. 2015..PTPN23, TAF6, TBCK, FAM177A1, KIAA1109, MTSS1L, XIRP1, KCTD3, CHAF1B, ARV1, ISCA2, PTRH2, GEMIN4, MYOCD, PDPR, DPH1, NUP107, TMEM92, EPB41L4A, and FAM120AOS)...
- Redox status of high-mobility group box 1 performs a dual role in angiogenesis of colorectal carcinomaLingyin Zhu
State Key Laboratory for Oncogenes and Related Genes, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Cancer Institute, Shanghai Institute of Digestive Disease, Shanghai, China
J Cell Mol Med 19:2128-35. 2015..the endothelial cells (ECs), at-HMGB1 or ds-HMGB1 was added to cell medium, further with their special inhibitors (DPH1.1 mAb and 2G7 mAb) and antibodies of corresponding receptors (RAGE Ab and TLR4 Ab)...
- Matching two independent cohorts validates DPH1 as a gene responsible for autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomaliesCatrina M Loucks
Department of Medical Genetics, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
Hum Mutat 36:1015-9. 2015..2015) identified 33 putative candidate disease genes for neurogenetic disorders. One such gene was DPH1, in which a homozygous missense mutation was associated with a 3C syndrome-like phenotype in four patients from a ..
- Methylation of the DPH1 promoter causes immunotoxin resistance in acute lymphoblastic leukemia cell line KOPN-8Xiaobo Hu
Laboratory of Molecular Biology, National Cancer Institute NCI, National Institutes of Health NIH, Bethesda, MD, USA
Leuk Res 37:1551-6. 2013..HA22-resistant KOPN-8 cells and found that HA22 cannot inactivate elongation factor-2 (EF2) due to low levels of DPH1 RNA and protein. Resistance was associated with methylation of the CpG island in the DPH1 promoter...
- Identification of genes induced by BRCA1 in breast cancer cellsArzu Atalay
Department of Molecular Biology and Genetics, Faculty of Science, Bilkent University, 06533 Ankara, Turkey
Biochem Biophys Res Commun 299:839-46. 2002..interacting protein ERBIN, meningioma-associated protein MAC30, and a candidate ovarian tumour-suppressor OVCA1. Northern and Western blot analyses confirmed that the expression of these five genes are up-regulated following ..
- Insights into diphthamide, key diphtheria toxin effectorWael Abdel-Fattah
Institut fur Biologie, FG Mikrobiologie, Universitat Kassel, Kassel, Germany
Toxins (Basel) 5:958-68. 2013..In budding yeast, diphthamide formation involves seven genes, DPH1-DPH7...
- The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction networkShanow Uthman
Department of Genetics, University of Leicester, Leicester, United Kingdom
PLoS Genet 9:e1003334. 2013..In Saccharomyces cerevisiae, the initial steps of diphthamide biosynthesis are well characterized and require the DPH1-DPH5 genes. However, the last pathway step-amidation of the intermediate diphthine to diphthamide-is ill-defined...
- IAPs contain an evolutionarily conserved ubiquitin-binding domain that regulates NF-kappaB as well as cell survival and oncogenesisMads Gyrd-Hansen
The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
Nat Cell Biol 10:1309-17. 2008..Our data identify IAPs as ubiquitin-binding proteins that contribute to ubiquitin-mediated cell survival, NF-kappaB signalling and oncogenesis...
- High throughput functional genomics: identification of novel genes with tumor suppressor phenotypesKerstin Koenig-Hoffmann
Xantos Biomedicine AG, Munich, Germany
Int J Cancer 113:434-9. 2005..TSGs or associated with tumor inhibitory activities (prostate differentiation factor, hRAS-like suppressor 3, DPH2L1-like and the metastasis inhibitor Kiss1) were confirmed in their proposed TSG-like phenotype by functionally ..
- OVCA1: emerging as a bona fide tumor suppressorMichael Rugaard Jensen
Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy
Genes Dev 18:245-8. 2004
- Protein degradation: recognition of ubiquitinylated substratesRasmus Hartmann-Petersen
August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen, Denmark
Curr Biol 14:R754-6. 2004A cell-free system has been developed in budding yeast that provides direct evidence that the Dsk2/Dph1, Rad23/Rhp23 and Rpn10/Pus1 multi-ubiquitin-binding proteins, long implicated in substrate recognition and presentation to the 26S ..
- Diphthamide modification on eukaryotic elongation factor 2 is needed to assure fidelity of mRNA translation and mouse developmentShihui Liu
Microbial Pathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 109:13817-22. 2012..Therefore, in contrast to mouse embryonic fibroblasts (MEFs) from OVCA1(-/-) mice, eEF2(G717R/G717R) MEFs retain full activity in polypeptide elongation and have normal growth rates...
- Pharmacokinetics and organ distribution of diarylheptanoid phytoestrogens from Curcuma comosa in ratsJian Su
Center for Research and Development of Herbal Health Products, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
J Nat Med 66:468-75. 2012..HPLC was used to measure the concentration of three major compounds, (6E)-1,7-diphenylhept-6-en-3-one (DPH1), (4E,6E)-1,7-diphenylhepta-4,6-dien-3-ol (DPH2), and (6E)-1,7-diphenylhept-6-en-3-ol (DPH3), which were found to ..
- OVCA1 inhibits the proliferation of epithelial ovarian cancer cells by decreasing cyclin D1 and increasing p16Fandou Kong
Department of Gynecology and Obstetrics, The First Affiliated Hospital, Dalian Medical University, Dalian, China
Mol Cell Biochem 354:199-205. 2011b>OVCA1, a tumor suppressor gene, is deleted or lower expressed in about 80% of ovarian cancer. Over expression of OVCA1 in human ovarian cancer A2780 cells inhibits cell proliferation and arrests cells in G1 stage...
- A dominant-negative approach that prevents diphthamide formation confers resistance to Pseudomonas exotoxin A and diphtheria toxinVincent Roy
Le Centre de Recherche en Cancérologie de l Université Laval, L Hôtel Dieu de Québec, Centre Hospitalier Universitaire de Quebec, Quebec, Canada
PLoS ONE 5:e15753. 2010..It is well documented that Dph1 and Dph2 are involved in the first biochemical step of diphthamide formation and that these two proteins interact ..
- Myosin V spatially regulates microtubule dynamics and promotes the ubiquitin-dependent degradation of the fission yeast CLIP-170 homologue, Tip1Rebeca Martín-García
School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK
J Cell Sci 122:3862-72. 2009..Myo52 facilitates microtubule catastrophe by enhancing Tip1 removal from the plus end of growing microtubules at the cell tips. There, Myo52 and the ubiquitin receptor, Dph1, work in concert to target Tip1 for degradation.
- Gene silencing in the marine diatom Phaeodactylum tricornutumValentina De Riso
Laboratory of Ecology and Evolution of Plankton, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
Nucleic Acids Res 37:e96. 2009..silencing of a GUS reporter gene expressed in transgenic lines, as well as the knockdown of endogenous phytochrome (DPH1) and cryptochrome (CPF1) genes...
- Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse developmentShihui Liu
Bacterial Toxins and Therapeutics Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Cell Biol 26:3835-41. 2006..Diphthamide biosynthesis is carried out by five highly conserved proteins, Dph1 to Dph5, and an as-yet-unidentified amidating enzyme...
- Diphthamide modification of eEF2 requires a J-domain protein and is essential for normal developmentTom R Webb
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, UK
J Cell Sci 121:3140-5. 2008..This enigmatic modification occurs in all eukaryotes and is produced in yeast by the action of five gene products, DPH1 to DPH5...
- A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3Christian Bär
Biologicum, Institut fur Genetik, Martin Luther Universitat Halle Wittenberg, Weinbergweg 10, D 06120 Halle Saale, Germany
Mol Microbiol 69:1221-33. 2008..The latter phenotype applies to all diphthamide mutants (dph1-dph5) tested and Kti11/Dph3 physically interacts with diphthamide synthesis factors Dph1 and Dph2, presumably as ..
- The diphthamide modification on elongation factor-2 renders mammalian cells resistant to ricinPradeep K Gupta
Laboratory of Bacterial Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell Microbiol 10:1687-94. 2008..Five proteins Dph1-Dph5 are required for the biosynthesis of diphthamide...
- Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3Carlos Cardoso
Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA
Am J Hum Genet 72:918-30. 2003....
- Identification of two candidate tumor suppressor genes on chromosome 17p13.3D C Schultz
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
Cancer Res 56:1997-2002. 1996..loss mapping and positional cloning methods, we have recently identified two novel genes, which we refer to as OVCA1 and OVCA2, that map to 17p13.3...
- Deletion mapping on chromosome 17p in medulloblastomaE Steichen Gersdorf
Department of Pediatrics, University of Innsbruck, Austria
Br J Cancer 76:1284-7. 1997..Candidate genes within this region are HIC-1, a potential tumour-suppressor gene, and DPH2L, a gene that has been cloned from the ovarian critical region...
- Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombeX He
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
J Cell Sci 111:1635-47. 1998..In the same screen which led to the isolation of mad2 and mph1, we also isolated dph1, a cDNA that encodes a protein 46% identical to an S. cerevisiae SPB duplication protein, Dsk2p...
- Cloning and localization of a human diphthamide biosynthesis-like protein-2 gene, DPH2L2D C Schultz
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, 19111, USA
Genomics 52:186-91. 1998Sequence analysis of the candidate tumor suppressor OVCA1 revealed extensive sequence identity and similarity to proteins from a diverse number of species, including the yeast diphthamide biosynthesis protein-2, dph2, which suggested ..
- Identification of XDRP1; a Xenopus protein related to yeast Dsk2p binds to the N-terminus of cyclin A and inhibits its degradationM Funakoshi
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812 8582, Japan
EMBO J 18:5009-18. 1999..significant homology in its C-terminal 50 residues to Saccharomyces cerevisiae Dsk2 and Schizosaccharomyces pombe dph1. XDRP1 is a nuclear phosphoprotein in Xenopus cells, and its phosphorylation is mediated by cyclin A-dependent ..
- Expression of OVCA1, a candidate tumor suppressor, is reduced in tumors and inhibits growth of ovarian cancer cellsW Bruening
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
Cancer Res 59:4973-83. 1999Loss of all or part of one copy of chromosome 17p is very common in ovarian and breast tumors. OVCA1 is a candidate tumor suppressor gene mapping to a highly conserved region on chromosome 17p13...
- Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressorA M Salicioni
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
Genomics 69:54-62. 2000The OVCA1 gene is a candidate for the breast and ovarian tumor suppressor gene at chromosome 17p13.3. To help determine the function(s) of OVCA1, we used a yeast two-hybrid screening approach to identify OVCA1-associating proteins...
- Cloning, structure, and expression of the mouse Ovca1 geneC M Chen
Department of Molecular Genetics, University of Texas, M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA
Biochem Biophys Res Commun 286:1019-26. 2001We report the isolation of the mouse Ovca1 gene, the orthologue of human OVCA1/DPH2L1, a putative tumor suppressor associated with ovarian cancer. Mouse Ovca1 contains at least 13 exons and spans approximately 17 kb...
- OVCA2 is downregulated and degraded during retinoid-induced apoptosisAmanda H Prowse
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
Int J Cancer 99:185-92. 2002..We have previously identified 2 genes, termed OVCA1 and OVCA2, which are located in a small region showing a high frequency of allelic loss in breast and ovarian ..
- Ubiquitin binding proteins protect ubiquitin conjugates from disassemblyRasmus Hartmann-Petersen
August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen O, Denmark
FEBS Lett 535:77-81. 2003..Here we report that three ubiquitin binding proteins, Rhp23, Dph1 and Pus1, from fission yeast can protect multiubiquitin conjugates against deubiquitination...
- Ovca1, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethalityMin Liang
Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA
Genes Chromosomes Cancer 47:315-25. 2008..We discovered a polymorphic change (321P-->321S) of Ovca1 within the Mop2 locus of CE/J mice...
- Molecular heterogeneity in human osteosarcoma demonstrated by enriched mRNAs isolated by directional tag PCR subtraction cloningOle K Olstad
Department of Medical Biochemistry, Norwegian Radium Hospital, 0310 N Oslo, Norway
Anticancer Res 23:2201-16. 2003..Alpha enolase, G-s-alpha, GPR85, DAF, RPL35A, GIF, TAPA-1, ANAPC11, DCI, hsp27, MRPS7 homolog, eIF p110 subunit, DPH2L, HMG-14, FB1 protein, chondroitin-6-sulphonase, calgizzarin, RNA polymerase II subunit, RPL13A, DHS, gp96, HHP2, ..
- Ovca1 regulates cell proliferation, embryonic development, and tumorigenesisChun Ming Chen
Department of Molecular Genetics, University of Texas, MD Anderson Cancer Center, Houston, Texas 77030, USA
Genes Dev 18:320-32. 2004Loss of OVCA1/DPH2L1 correlates with ovarian and breast cancer. To study its in vivo role, we generated Ovca1 mutant alleles in mice. Ovca1 heterozygotes spontaneously develop cancer...
- Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2Shihui Liu
Microbial Pathogenesis Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell Biol 24:9487-97. 2004..The human tumor suppressor gene OVCA1, previously identified as homologous to yeast DPH2, is shown to actually be HsDPH1...
- Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2Yoshitaka Nobukuni
Department of Human Genetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8553, Japan
J Biol Chem 280:10572-7. 2005b>OVCA1 is a tumor suppressor identified by positional cloning from chromosome 17p13.3, a hot spot for chromosomal aberration in breast and ovarian cancers...
- OVCA1: tumor suppressor geneChun Ming Chen
Faculty of Life Sciences, National Yang Ming University, 155 Li Nong Street, Section 2, Shihpai, Taipei 112, Taiwan
Curr Opin Genet Dev 15:49-54. 2005OVCA1, also known as DPH2L1, is a tumor suppressor gene associated with ovarian carcinoma and other tumors. Ovca1 homozygous mutant mice die at birth with developmental delay and cell-autonomous proliferation defects...
- [Chromosome arm 17p13.3: could HIC1 be the one ?]Valérie Chopin
ERI 8 INSERM, Signalisation des facteurs de croissance dans le cancer du sein, Protéomique fonctionnelle, UPRES EA 1033, IFR 118, Batiment SN3, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
Med Sci (Paris) 22:54-61. 2006..3, HIC1 (hypermethylated in cancer 1) and OVCA1 (ovarian cancer gene 1)...
- White spot syndrome virus open reading frame 222 encodes a viral E3 ligase and mediates degradation of a host tumor suppressor via ubiquitinationFang He
Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Republic of Singapore
J Virol 80:3884-92. 2006..revealed that WSSV222 interacts with a shrimp tumor suppressor-like protein (TSL) sharing 60% identity with human OVCA1. To better characterize the interaction of WSSV222 and TSL in vivo, we established a stable TSL-expressing cell ..
- A cDNA from the ovarian cancer critical region of deletion on chromosome 17p13.3N J Phillips
Department of Pathology, Saint Louis University School of Medicine, MO 63110, USA
Cancer Lett 102:85-90. 1996..This gene, named DPH2L (diphthamide biosynthesis protein 2-like), is expressed in multiple tissues and stages of development.
- Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 -/- epithelial ovarian cancersStefano Indraccolo
Istituto Oncologico Veneto, Padova, Italy
Eur J Cancer 42:1475-83. 2006..We derived two ovarian cancer cell lines, termed PD-OVCA1 and PD-OVCA2, both from patients previously treated with chemotherapy, that propagate in SCID mice as well as in ..