DNA ligase IV

Summary

Gene Symbol: DNA ligase IV
Description: DNA ligase 4
Alias: LIG4S, DNA ligase 4, DNA joinase, DNA ligase IV, DNA repair enzyme, ligase IV, DNA, ATP-dependent, polydeoxyribonucleotide synthase [ATP] 4, polynucleotide ligase, sealase
Species: human

Top Publications

  1. pmc Dysbindin-1, a schizophrenia-related protein, functionally interacts with the DNA- dependent protein kinase complex in an isoform-dependent manner
    Satoko Oyama
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro ku, Tokyo, Japan
    PLoS ONE 4:e4199. 2009
  2. pmc Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination
    Y F Wei
    Human Genome Sciences, Inc, Rockville, Maryland 20850 3338, USA
    Mol Cell Biol 15:3206-16. 1995
  3. ncbi Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells
    U Grawunder
    Washington University School of Medicine, Division of Molecular Oncology, Department of Pathology, St Louis, Missouri 63110, USA
    Nature 388:492-5. 1997
  4. ncbi Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV
    S E Critchlow
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 7:588-98. 1997
  5. ncbi DNA ligase IV binds to XRCC4 via a motif located between rather than within its BRCT domains
    U Grawunder
    University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles 90033, USA
    Curr Biol 8:873-6. 1998
  6. ncbi DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes
    U Grawunder
    University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles 90033, USA
    Mol Cell 2:477-84. 1998
  7. ncbi Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient
    E Riballo
    MRC Cell Mutation Unit, University of Sussex, Brighton, BN1 9RR, UK
    Curr Biol 9:699-702. 1999
  8. ncbi Cellular and biochemical impact of a mutation in DNA ligase IV conferring clinical radiosensitivity
    E Riballo
    Medical Research Council, Cell Mutation Unit, University of Sussex, Brighton BN1 9RR, United Kingdom
    J Biol Chem 276:31124-32. 2001
  9. ncbi Crystal structure of an Xrcc4-DNA ligase IV complex
    B L Sibanda
    Department of Biochemistry, Cambridge University, Tennis Court Road, Cambridge CB2 1GA, UK
    Nat Struct Biol 8:1015-9. 2001
  10. ncbi DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency
    M O'Driscoll
    Genome Damage and Stability Unit, University of Sussex, Brighton, East Sussex, BN1 9RR, United Kingdom
    Mol Cell 8:1175-85. 2001

Research Grants

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
  2. SAMY LEWIZ HABIB; Fiscal Year: 2016
  3. Leona D Samson; Fiscal Year: 2016
  4. DNA Damage and Neurodegeneration in the Aging Brain
    Bruce A Yankner; Fiscal Year: 2010
  5. Sandeep Burma; Fiscal Year: 2015
  6. Catherine T Yan; Fiscal Year: 2016
  7. Impact of genetic variation on response to GO therapy in COG-AML clinical trials
    ROLAND BRUNO WALTER; Fiscal Year: 2013
  8. Alan E Tomkinson; Fiscal Year: 2014
  9. The Roles of Trm9 and tRNA Methylation in the DNA Damage Response
    Thomas J Begley; Fiscal Year: 2010
  10. SHEILA SUE DAVID; Fiscal Year: 2016

Detail Information

Publications213 found, 100 shown here

  1. pmc Dysbindin-1, a schizophrenia-related protein, functionally interacts with the DNA- dependent protein kinase complex in an isoform-dependent manner
    Satoko Oyama
    Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Meguro ku, Tokyo, Japan
    PLoS ONE 4:e4199. 2009
    ....
  2. pmc Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination
    Y F Wei
    Human Genome Sciences, Inc, Rockville, Maryland 20850 3338, USA
    Mol Cell Biol 15:3206-16. 1995
    ..A fourth DNA ligase (DNA ligase IV) has been purified from human cells and shown to be identical to the 96-kDa DNA ligase by unique agreement ..
  3. ncbi Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells
    U Grawunder
    Washington University School of Medicine, Division of Molecular Oncology, Department of Pathology, St Louis, Missouri 63110, USA
    Nature 388:492-5. 1997
    ..Here we show that DNA ligase IV co-immunoprecipitates with XRCC4 and that these two proteins specifically interact with one another in a yeast ..
  4. ncbi Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV
    S E Critchlow
    Wellcome CRC Institute, Cambridge, UK
    Curr Biol 7:588-98. 1997
    ..These features are shared by cells lacking components of the DNA-dependent protein kinase (DNA-PK). Although the XRCC4 gene has been cloned, the function(s) of XRCC4 in DNA end-joining has remained elusive...
  5. ncbi DNA ligase IV binds to XRCC4 via a motif located between rather than within its BRCT domains
    U Grawunder
    University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles 90033, USA
    Curr Biol 8:873-6. 1998
    ..It has recently been demonstrated that DNA ligase IV interacts with and is catalytically stimulated by the XRCC4 protein [2,3], which is essential for DNA double-..
  6. ncbi DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes
    U Grawunder
    University of Southern California School of Medicine, Norris Comprehensive Cancer Center, Department of Pathology, Los Angeles 90033, USA
    Mol Cell 2:477-84. 1998
    ..Here we show that the targeted disruption of both DNA ligase IV alleles in a human pre-B cell line renders the cells sensitive to ionizing radiation and ablates V(D)J ..
  7. ncbi Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient
    E Riballo
    MRC Cell Mutation Unit, University of Sussex, Brighton, BN1 9RR, UK
    Curr Biol 9:699-702. 1999
    ..non-homologous end-joining (NHEJ), a process that involves the DNA-dependent protein kinase [1] [2], XRCC4 and DNA ligase IV [3] [4] [5] [6]...
  8. ncbi Cellular and biochemical impact of a mutation in DNA ligase IV conferring clinical radiosensitivity
    E Riballo
    Medical Research Council, Cell Mutation Unit, University of Sussex, Brighton BN1 9RR, United Kingdom
    J Biol Chem 276:31124-32. 2001
    b>DNA ligase IV functions in DNA non-homologous end-joining, in V(D)J recombination, and during brain development...
  9. ncbi Crystal structure of an Xrcc4-DNA ligase IV complex
    B L Sibanda
    Department of Biochemistry, Cambridge University, Tennis Court Road, Cambridge CB2 1GA, UK
    Nat Struct Biol 8:1015-9. 2001
    A complex of two proteins, Xrcc4 and DNA ligase IV, plays a fundamental role in DNA non-homologous end joining (NHEJ), a cellular function required for double-strand break repair and V(D)J recombination...
  10. ncbi DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency
    M O'Driscoll
    Genome Damage and Stability Unit, University of Sussex, Brighton, East Sussex, BN1 9RR, United Kingdom
    Mol Cell 8:1175-85. 2001
    b>DNA ligase IV functions in DNA nonhomologous end-joining and V(D)J recombination...
  11. ncbi Variants in DNA double-strand break repair genes and breast cancer susceptibility
    Bettina Kuschel
    Cancer Research UK Department of Oncology, University of Cambridge, Strangeways Research Laboratory, Worts Causeway, Cambridge CB1 8RN, UK
    Hum Mol Genet 11:1399-407. 2002
    ..If these results can be confirmed, understanding the functional basis should improve our understanding of the role of DNA repair in breast carcinogenesis...
  12. ncbi DNA ligase IV-dependent NHEJ of deprotected mammalian telomeres in G1 and G2
    Agata Smogorzewska
    Laboratory for Cell Biology and Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Curr Biol 12:1635-44. 2002
    ..However, the molecular nature of these fusions and the mechanism by which they occur have not been elucidated...
  13. pmc Genetic variants of NHEJ DNA ligase IV can affect the risk of developing multiple myeloma, a tumour characterised by aberrant class switch recombination
    P L Roddam
    Academic Unit of Haematology and Oncology, University of Leeds, Algernon Firth Building, Leeds, West Yorkshire LS2 9JT, UK
    J Med Genet 39:900-5. 2002
    ..We investigated the potential impact that previously undescribed polymorphisms identified within NHEJ DNA ligase IV (LIG4) have upon predisposition to several lymphoproliferative disorders, including leukaemia, lymphoma, and ..
  14. ncbi Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment
    Patrick Calsou
    Institut de Pharmacologie et de Biologie Structurale, CNRS UMR 5089, 205 route de Narbonne, 31077, Cedex 4, Toulouse, France
    J Mol Biol 326:93-103. 2003
    ..These findings extend the current knowledge of the assembly of NHEJ repair proteins on DNA termini and substantiate the hypothesis of a scaffolding role of DNA-PK towards other components of the NHEJ DNA repair process...
  15. pmc In vitro and in vivo interactions of DNA ligase IV with a subunit of the condensin complex
    Marcin R Przewloka
    Los Alamos National Laboratory, Biosciences Division, New Mexico 87545, USA
    Mol Biol Cell 14:685-97. 2003
    Several findings have revealed a likely role for DNA ligase IV, and interacting protein XRCC4, in the final steps of mammalian DNA double-strand break repair...
  16. pmc Impact of DNA ligase IV on the fidelity of end joining in human cells
    Julianne Smith
    UMR 218 CNRS, Institut Curie Recherche, 26 Rue d Ulm, 75248 Paris, France
    Nucleic Acids Res 31:2157-67. 2003
    A DNA ligase IV (LIG4)-null human pre-B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay...
  17. ncbi Breast cancer risk associated with genotypic polymorphism of the nonhomologous end-joining genes: a multigenic study on cancer susceptibility
    Yi Ping Fu
    Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan
    Cancer Res 63:2440-6. 2003
    ....
  18. ncbi Polymorphisms in DNA double-strand break repair genes and breast cancer risk in the Nurses' Health Study
    Jiali Han
    Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Carcinogenesis 25:189-95. 2004
    ..The gene-environment interaction data suggest that the subtle effects of some of these variants on breast cancer risk may be magnified in the presence of certain exposures...
  19. ncbi Analysis of DNA ligase IV mutations found in LIG4 syndrome patients: the impact of two linked polymorphisms
    Pierre Marie Girard
    Genome Damage and Stability Centre, University of Sussex, East Sussex BN1 9RQ, UK
    Hum Mol Genet 13:2369-76. 2004
    LIG4 syndrome patients have hypomorphic mutations in DNA ligase IV. Although four of the five identified patients display immunodeficiency and developmental delay, one patient was developmentally normal...
  20. ncbi Association of amino acid substitution polymorphisms in DNA repair genes TP53, POLI, REV1 and LIG4 with lung cancer risk
    Tokuki Sakiyama
    Center for Medical Genomics, National Cancer Center Research Institute, Tokyo, Japan
    Int J Cancer 114:730-7. 2005
    ..The present results suggest that these 4 SNPs function as genetic factors underlying lung cancer susceptibility by modulating activities to maintain the genome integrity of each individual...
  21. ncbi Genetic polymorphisms of selected DNA repair genes, estrogen and progesterone receptor status, and breast cancer risk
    Kyoung Mu Lee
    Department of Preventive Medicine, Seoul National University College of Medicine, Chongno Gu, Seoul, Korea
    Clin Cancer Res 11:4620-6. 2005
    ..To evaluate the role of genetic polymorphisms of DNA repair genes in breast cancer, we conducted a hospital-based case-control study of Korean women...
  22. pmc A new type of radiosensitive T-B-NK+ severe combined immunodeficiency caused by a LIG4 mutation
    Mirjam van der Burg
    Department of Immunology and Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands
    J Clin Invest 116:137-45. 2006
    ..We present a patient with a new type of radiosensitive T-B-NK+ SCID with a defect in DNA ligase IV (LIG4)...
  23. ncbi XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining
    Peter Ahnesorg
    The Gurdon Institute, Cambridge University, UK
    Cell 124:301-13. 2006
    ..Central to NHEJ is the protein complex containing DNA Ligase IV and XRCC4...
  24. ncbi Polymorphisms in DNA double-strand break repair genes and risk of breast cancer: two population-based studies in USA and Poland, and meta-analyses
    Montserrat Garcia-Closas
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services, 6120 Executive Boulevard, Room 7076, Rockville, MD 20952 7234, USA
    Hum Genet 119:376-88. 2006
    ..Evaluation of potential underlying gene-gene interactions or associations in population subgroups will require even larger sample sizes...
  25. ncbi Identification of a novel motif in DNA ligases exemplified by DNA ligase IV
    Caterina Marchetti
    Genome Damage and Stability Centre, University of Sussex, BN9 1RQ UK
    DNA Repair (Amst) 5:788-98. 2006
    b>DNA ligase IV is an essential protein that functions in DNA non-homologous end-joining, the major mechanism that rejoins DNA double-strand breaks in mammalian cells...
  26. pmc Risk of non-Hodgkin lymphoma (NHL) in relation to germline variation in DNA repair and related genes
    Deirdre A Hill
    Cancer Center and Department of Internal Medicine, UNM School of Medicine, 1 University of New Mexico, MSC 10 5550, Albuquerque, NM 87131 0001, USA
    Blood 108:3161-7. 2006
    ..001), and less likely to have the LIG4 (DNA ligase IV) 9 Ile/Ile (OR = 0.5; 95% CI = 0.3 to 0.9) than T/T genotype (P trend = ...
  27. ncbi Interaction of the Ku heterodimer with the DNA ligase IV/Xrcc4 complex and its regulation by DNA-PK
    Silvia Costantini
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I 34012 Trieste, Italy
    DNA Repair (Amst) 6:712-22. 2007
    ..we characterize the interaction between two key components of the NHEJ machinery, the Ku heterodimer and the DNA ligase IV/Xrcc4 complex...
  28. ncbi Polymorphisms in the DNA ligase IV gene might influence the risk of acute lymphoblastic leukemia in children
    J Andreae
    Leukemia 21:2226-7. 2007
  29. pmc Modes of interaction among yeast Nej1, Lif1 and Dnl4 proteins and comparison to human XLF, XRCC4 and Lig4
    Rajashree A Deshpande
    Department of Pathology, University of Michigan Medical School, Room 2065 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109 2200, USA
    DNA Repair (Amst) 6:1507-16. 2007
    The nonhomologous end joining (NHEJ) pathway of double-strand break repair depends on DNA ligase IV and its interacting partner protein XRCC4 (Lif1 in yeast)...
  30. doi Polymorphisms of LIG4 and XRCC4 involved in the NHEJ pathway interact to modify risk of glioma
    Yanhong Liu
    State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China
    Hum Mutat 29:381-9. 2008
    ..Further dendrogram and graph analysis indicated a more-than-additive effect among these three loci. These results suggested that these variants may contribute to glioma susceptibility...
  31. pmc DNA-ligase IV and DNA-protein kinase play a critical role in deficient caspases activation in apoptosis-resistant cancer cells by using doxorubicin
    Claudia Friesen
    Institute of Legal Medicine, University of Ulm, 89075 Ulm, Germany
    Mol Biol Cell 19:3283-9. 2008
    ..Failure in caspases activation using doxorubicin depends on loss of DNA damage and is due to higher rates of NHEJ-DNA-DBS-repair...
  32. pmc Validating genetic risk associations for ovarian cancer through the international Ovarian Cancer Association Consortium
    C L Pearce
    Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Br J Cancer 100:412-20. 2009
    ..CYP3A4 encodes a key enzyme in oestrogen metabolism and our finding between rs2740574 and risk of ovarian cancer suggests that this pathway may be involved in ovarian carcinogenesis. Additional follow-up is warranted...
  33. pmc DNA repair gene polymorphisms and risk of pancreatic cancer
    Donghui Li
    Department of Gastrointestinal Medical Oncology, M D Anderson Cancer Center, 1515 Holcombe Boulevard, Box 426, Houston, TX 77030, USA
    Clin Cancer Res 15:740-6. 2009
    ..The current research was undertaken to examine the association between genetic variations in DNA repair and pancreatic cancer risk...
  34. doi Acute normal tissue reactions in head-and-neck cancer patients treated with IMRT: influence of dose and association with genetic polymorphisms in DNA DSB repair genes
    Joke Werbrouck
    Department of Basic Medical Sciences, Ghent University, Ghent, Belgium
    Int J Radiat Oncol Biol Phys 73:1187-95. 2009
    ..1843A>G, c.562-14A>G, c.722C>T), Rad51 (c.-3429G>C, c.-3392G>T), Lig4 (c.26C>T, c.1704T>C), Ku70 (c.-1310C>G), and Ku80 (c.2110-2408G>A) and the occurrence of acute reactions after radiotherapy...
  35. pmc Structural and functional interaction between the human DNA repair proteins DNA ligase IV and XRCC4
    Pei Yu Wu
    CNRS, IPBS Institut de Pharmacologie et de Biologie Structurale, Toulouse, France
    Mol Cell Biol 29:3163-72. 2009
    ..It relies on the XRCC4/DNA ligase IV complex to reseal DNA strands...
  36. doi Lung cancer susceptibility and prognosis associated with polymorphisms in the nonhomologous end-joining pathway genes: a multiple genotype-phenotype study
    Ruo Chia Tseng
    Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Cancer 115:2939-48. 2009
    ..The authors of this report postulated that polymorphisms in genes of the nonhomologous end-joining (NHEJ) pathway, which is the major DSB repair pathway in mammalian cells, may modulate lung cancer susceptibility and prognosis...
  37. doi A novel mutation in a family with DNA ligase IV deficiency syndrome
    Sule Unal
    Division of Pediatric Hematology, Faculty of Medicine, Department of Pediatrics, Hacettepe University, 06100 Sihhiye, Ankara, Turkey
    Pediatr Blood Cancer 53:482-4. 2009
    b>DNA ligase IV deficiency syndrome (LIG4 syndrome) is a rare autosomal recessive disorder characterized by microcephaly, growth retardation, low birth weight, dysmorphic facial findings, immunodeficiency, pancytopenia, and ..
  38. pmc Distinct kinetics of human DNA ligases I, IIIalpha, IIIbeta, and IV reveal direct DNA sensing ability and differential physiological functions in DNA repair
    Xi Chen
    Department of Radiation Oncology and Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    DNA Repair (Amst) 8:961-8. 2009
    ..step of the next ligation reaction and suggests that, unless there is an in vivo mechanism to reactivate DNA ligase IV/XRCC4 following phosphodiester bond formation, the cellular NHEJ capacity will be determined by the number of ..
  39. doi Electron microscopy of Xrcc4 and the DNA ligase IV-Xrcc4 DNA repair complex
    María A Recuero-Checa
    Centro de Investigaciones Biologicas CIB, Consejo Superior de Investigaciones Cientificas CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
    DNA Repair (Amst) 8:1380-9. 2009
    The DNA ligase IV-Xrcc4 complex is responsible for the ligation of broken DNA ends in the non-homologous end-joining (NHEJ) pathway of DNA double strand break repair in mammals...
  40. pmc Polymorphisms of LIG4, BTBD2, HMGA2, and RTEL1 genes involved in the double-strand break repair pathway predict glioblastoma survival
    Yanhong Liu
    Departments of Epidemiology, Neuro Oncology, and Pathology, TheUniversity of Texas MD Anderson CancerCente, Houston, TX 77030, USA
    J Clin Oncol 28:2467-74. 2010
    ..Therefore, identifying the genetic variants that influence this small number of unusually long-term survivors may provide important insight into tumor biology and treatment...
  41. doi The LIG4 Ile658Val polymorphism does not affect the risk of cervical carcinoma
    Zhiming Ding
    Department of Gynecologic Oncology, Women s Hospital, School of Medicine, Zhejiang University, Xueshi Rd 2, Hangzhou 310006, China
    Pathol Res Pract 206:556-9. 2010
    ..In conclusion, in the Chinese population, LIG4 Ile658Val has only a slight impact on the risk of developing cervical carcinoma...
  42. ncbi Analysis of the polymorphisms in non-homologous DNA end joining (NHEJ) gene Ku70 and Ligase IV in sporadic breast cancer in women
    Anna Sobczuk
    Beata Smolarz MD, PhD, Institute of Polish Mother s Memorial Hospital, ul Rzgowska 281 289, 93 338 Łódź, E mail
    Pol J Pathol 61:27-31. 2010
    ..Double-stranded breaks are repaired in two pathways: homologous recombination (HR) and non-homologous DNA end joining (NHEJ). It is not known whether these repair pathways are affected in sporadic breast tumours...
  43. ncbi The role of common variants of non-homologous end-joining repair genes XRCC4, LIG4 and Ku80 in thyroid cancer risk
    Bruno Costa Gomes
    Department of Genetics, Faculty of Medical Sciences, Universidade Nova de Lisboa, 1349 008 Lisboa, Portugal
    Oncol Rep 24:1079-85. 2010
    ..063-4.894; P=0.034). Taken together these results suggest that some of these variants in NHEJ genes can contribute to thyroid cancer susceptibility. However, further studies with a larger sample size will be needed to support our results...
  44. pmc Genetic variants of the nonhomologous end joining gene LIG4 and severe radiation pneumonitis in nonsmall cell lung cancer patients treated with definitive radiotherapy
    Ming Yin
    Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA
    Cancer 118:528-35. 2012
    ..The authors hypothesized that single nucleotide polymorphisms (SNPs) in NHEJ repair genes may affect clinical outcomes in patients with nonsmall cell lung cancer (NSCLC) who receive definitive radio(chemo)therapy...
  45. doi DNA repair gene polymorphisms and risk of early onset colorectal cancer in Lynch syndrome
    Stuart G Reeves
    Discipline of Medical Genetic, Faculty of Health, University of Newcastle, NSW, Australia
    Cancer Epidemiol 36:183-9. 2012
    ..The data suggest that polymorphisms in DNA repair genes do not contribute to cancer risk in a population of CRC patients who are at increased risk of disease as a result in a deficiency of DNA mismatch repair...
  46. doi XRCC4 controls nuclear import and distribution of Ligase IV and exchanges faster at damaged DNA in complex with Ligase IV
    Elke Berg
    Institute of Clinical Chemistry and Laboratory Diagnostics, Univ Dusseldorf, Medical Faculty, D 40225 Dusseldorf, Germany
    DNA Repair (Amst) 10:1232-42. 2011
    ..Following break recognition, alignment and processing, broken DNA ends are finally rejoined by the essential DNA Ligase IV. In the cell, Ligase IV is unable to function without its constitutive interaction partner XRCC4 and becomes ..
  47. ncbi Lack of association between LIG4 gene polymorphisms and the risk of breast cancer: a HuGE review and meta-analysis
    Li ping Zhou
    Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, China
    Asian Pac J Cancer Prev 13:3417-22. 2012
    ..The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to investigate association between LIG4 gene polymorphisms in the NHEJ pathway and breast cancer risk...
  48. pmc Genetic polymorphisms of DNA double-strand break repair pathway genes and glioma susceptibility
    Peng Zhao
    Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China
    BMC Cancer 13:234. 2013
    ..We studied whether polymorphisms in DNA double-strand break repair genes are associated with an increased risk of glioma development...
  49. ncbi Structure and function of mammalian DNA ligases
    A E Tomkinson
    Department of Molecular Medicine, University of Texas Health Science Center at San Antonio 78245, USA
    Mutat Res 407:1-9. 1998
    ..Complex formation between DNA ligase IV and the DNA repair protein XRCC4 involves the C-terminal region of DNA ligase IV, which contains two BRCT ..
  50. pmc DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair
    Yankun Gao
    Department of Genetics, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Nature 471:240-4. 2011
    ..Therefore, our data reveal that the critical biological role of Lig3 is to maintain mtDNA integrity and not Xrcc1-dependent DNA repair...
  51. pmc A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks
    P Schär
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, UK
    Genes Dev 11:1912-24. 1997
    ..This DNA ligase shares significant amino acid sequence homology with human DNA ligase IV; accordingly, we designate the yeast gene LIG4...
  52. pmc Biochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe
    Ingrun Alseth
    Institute of Medical Microbiology, University of Oslo, The National Hospital N 0027 Oslo, Norway
    Nucleic Acids Res 33:1123-31. 2005
    The Schizosaccharomyces pombe mag1 gene encodes a DNA repair enzyme with sequence similarity to the AlkA family of DNA glycosylases, which are essential for the removal of cytotoxic alkylation products, the premutagenic deamination ..
  53. ncbi Knockdown of DNA ligase IV/XRCC4 by RNA interference inhibits herpes simplex virus type I DNA replication
    Isabella Muylaert
    Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Goteborg University, SE 405 30 Goteborg, Sweden
    J Biol Chem 282:10865-72. 2007
    ..3.N fibroblasts. We find that RNAi-mediated knockdown of DNA ligase IV and its co-factor XRCC4 causes a hundred-fold reduction of virus yield, a small plaque phenotype, and reduced ..
  54. ncbi Human DNA ligase IV and the ligase IV/XRCC4 complex: analysis of nick ligation fidelity
    Yu Wang
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 46:4962-76. 2007
    ..DNA repair) have been shown to be relatively intolerant of nicks containing mismatched base pairs, the human DNA ligase IV/XRCC4 complex has not been studied in this regard...
  55. pmc Identification of Saccharomyces cerevisiae DNA ligase IV: involvement in DNA double-strand break repair
    S H Teo
    Wellcome CRC Institute and Department of Zoology, University of Cambridge, UK
    EMBO J 16:4788-95. 1997
    ..and characterization of a novel gene, LIG4, which encodes a protein with strong homology to mammalian DNA ligase IV. Unlike CDC9, LIG4 is not essential for DNA replication, RAD52-dependent homologous recombination nor the ..
  56. ncbi Yeast DNA ligase IV mediates non-homologous DNA end joining
    T E Wilson
    Washington University School of Medicine, Division of Laboratory Medicine, Department of Pathology, St Louis, Missouri 63110, USA
    Nature 388:495-8. 1997
    ..cerevisiae has two typical DNA ligases, the known DNA ligase I homologue CDC9 and the previously unknown DNA ligase IV homologue DNL4. dnl4 mutants are deficient in precise and end-processed NHEJ...
  57. ncbi Targeted disruption of the gene encoding DNA ligase IV leads to lethality in embryonic mice
    D E Barnes
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Curr Biol 8:1395-8. 1998
    b>DNA ligase IV is the most recently identified member of a family of enzymes joining DNA strand breaks in mammalian cell nuclei [1] [2]...
  58. ncbi The HIV-1 transactivator protein Tat is a potent inducer of the human DNA repair enzyme beta-polymerase
    D K Srivastava
    Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    AIDS 15:433-40. 2001
    ..The rationale for these experiments is to examine the potential involvement of base-excision repair protein deregulation in HIV-1-related lymphomas...
  59. pmc DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: Implications for DNA double-strand break repair
    N Adachi
    Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, Totsuka ku, Yokohama 244 0813, USA
    Proc Natl Acad Sci U S A 98:12109-13. 2001
    ..To investigate the role of DNA ligase IV (Lig4) in DSB repair, we knocked out the Lig4 gene (LIG4) in the DT40 chicken B-lymphocyte cell line...
  60. pmc The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA
    Haico van Attikum
    Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    Nucleic Acids Res 31:4247-55. 2003
    ..In mammals and yeast, DNA ligase IV is exclusively involved in the repair of DNA double-strand breaks by non-homologous end joining...
  61. pmc Crucial role for DNA ligase III in mitochondria but not in Xrcc1-dependent repair
    Deniz Simsek
    Developmental Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 471:245-8. 2011
    ..Lig3-null cells are not sensitive to several DNA-damaging agents that sensitize Xrcc1-deficient cells. Our results establish a role for Lig3 in mitochondria, but distinguish it from its interacting protein Xrcc1...
  62. ncbi Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV
    K M Frank
    The Children s Hospital, Department of Genetics, Harvard University Medical School, Boston, Massachusetts 02115, USA
    Nature 396:173-7. 1998
    ..The precise function of XRCC4 is unknown, but it interacts with DNA ligase IV. Ligase IV is one of the three known mammalian DNA ligases; however, the in vivo functions of these ligases ..
  63. pmc Evidence for the involvement of DNA repair enzyme NEIL1 in nucleotide excision repair of (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines
    Pawel Jaruga
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA
    Biochemistry 49:1053-5. 2010
    The DNA repair enzyme NEIL1 is a DNA glycosylase that is involved in the first step of base excision repair (BER) of oxidatively induced DNA damage...
  64. ncbi The human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) DNA repair enzyme and its association with lung cancer risk
    Jong Park
    Divisions of Cancer Control and Molecular Oncology, H Lee Moffitt Cancer Center, University of South Florida, Tampa, FL 33612, USA
    Pharmacogenetics 14:103-9. 2004
    ..The codon 326 polymorphism in the hOGG1 gene has been suggested to reduce DNA repair enzyme activity based on in vitro functional analysis...
  65. pmc Absence of MGMT promoter methylation in endometrial cancer
    B J Rimel
    Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Washington University School of Medicine, USA
    Gynecol Oncol 112:224-8. 2009
    ..Loss of expression of DNA repair enzyme MGMT in oral leukoplakia and early oral squamous cell carcinoma...
  66. ncbi Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway
    Ryuichi Okayasu
    International Space Radiation Laboratory, National Institute of Radiological Sciences, Inage Ku, Chiba shi 263 8555, Japan
    Radiat Res 165:59-67. 2006
    ..NHEJ-deficient xrs6 (a Ku80 mutant of CHO) and 180BR human fibroblast (DNA ligase IV mutant) cells showed similar cell survival for X and carbon-ion irradiation (RBE = approximately 1.0)...
  67. ncbi Expression of redox factor-1, p53-activated gene 608 and caspase-3 messenger RNAs following repeated unilateral common carotid artery occlusion in gerbils--relationship to delayed cell injury and secondary failure of energy state
    D M Hermann
    Department of Experimental Neurology, Max Planck Institute for Neurological Research, Cologne, Germany
    Neuroscience 102:779-87. 2001
    The temporospatial expression pattern of the nuclear DNA repair enzyme redox factor-1 (ref-1), the p53-activated gene (pag) 608 and the effector caspase-3 was examined by in situ hybridization histochemistry in gerbils subjected to two ..
  68. ncbi Raman spectroscopy of uracil DNA glycosylase-DNA complexes: insights into DNA damage recognition and catalysis
    J Dong
    Department of Biochemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106 4935, USA
    Biochemistry 39:13241-50. 2000
    Using off-resonance Raman spectroscopy, we have examined each complex along the catalytic pathway of the DNA repair enzyme uracil DNA glycosylase (UDG)...
  69. ncbi Expression and activity of the DNA repair enzyme uracil DNA glycosylase during organogenesis in the rat conceptus and following methotrexate exposure in vitro
    Robert K Vinson
    Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir William Osler, Montreal, Que, Canada H3G 1Y6
    Biochem Pharmacol 64:711-21. 2002
    ..Furthermore, UNG expression was developmental stage- and tissue-specific; the discrepancy between transcript and protein levels suggests post-transcriptional regulation...
  70. pmc Expression and biochemical characterization of the Plasmodium falciparum DNA repair enzyme, flap endonuclease-1 (PfFEN-1)
    Louis J Casta
    Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107 6731, USA
    Mol Biochem Parasitol 157:1-12. 2008
    ..Plasmodium FEN-1s have enzymatic activities similar to other species but contain extended C-termini and a more internally located PCNA-binding site...
  71. ncbi Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates
    Mary Ann Pope
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84103, USA
    J Biol Chem 277:22605-15. 2002
    The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG)...
  72. doi A defect of LigD (human Lig4 homolog) for nonhomologous end joining significantly improves efficiency of gene-targeting in Aspergillus oryzae
    Osamu Mizutani
    Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Fungal Genet Biol 45:878-89. 2008
    ..oryzae, we constructed disrupted strains harboring a gene (ligD) encoding human DNA ligase IV homolog that is involved in the final step of DNA nonhomologous end joining. The A...
  73. ncbi Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains
    Y Ono
    Department of Neurological Surgery, Okayama University Medical School, Japan
    Brain Res Dev Brain Res 86:1-6. 1995
    ..stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities...
  74. ncbi Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III
    T P Hilbert
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, New York, New York 10016, USA
    J Biol Chem 272:6733-40. 1997
    ....
  75. ncbi Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta
    Bidisha Bose-Basu
    Laboratory of Structural Biology, NIEHS, NIH, Research Triangle Park, North Carolina 27709, USA
    Biochemistry 43:8911-22. 2004
    ..Thus, the NMR data indicate that the nucleotide-DNA interaction appears to be essential for conformational activation...
  76. ncbi Uracil misincorporation into DNA of leukocytes of young women with positive folate balance depends on plasma vitamin B12 concentrations and methylenetetrahydrofolate reductase polymorphisms. A pilot study
    Maria Kapiszewska
    Department of General Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, 30 387 Poland
    J Nutr Biochem 16:467-78. 2005
    ..In our study, the level of UrMis into DNA was evaluated by the comet assay (using the specific DNA repair enzyme, uracil DNA glycosylase) in leukocytes from blood donated by healthy young women with positive folate ..
  77. ncbi Endonuclease V protects Escherichia coli against specific mutations caused by nitrous acid
    K A Schouten
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mutat Res 435:245-54. 1999
    Endonuclease V (deoxyinosine 3'-endonuclease) of Escherichia coli K-12 is a putative DNA repair enzyme that cleaves DNA's containing hypoxanthine, uracil, or mismatched bases...
  78. ncbi Effect of topically applied T4 endonuclease V in liposomes on skin cancer in xeroderma pigmentosum: a randomised study. Xeroderma Pigmentosum Study Group
    D Yarosh
    Applied Genetics Incorporated Dermatics, Freeport, NY 11520, USA
    Lancet 357:926-9. 2001
    ..The bacterial DNA repair enzyme, T4 endonuclease V, delivered intracellularly, increases the rate of repair of sunlight-induced DNA damage ..
  79. ncbi Adenine release is fast in MutY-catalyzed hydrolysis of G:A and 8-Oxo-G:A DNA mismatches
    Joe A B McCann
    Department of Biochemistry, McMaster University, Hamilton, Ontario L8S 4M1, Canada
    J Biol Chem 278:29587-92. 2003
    MutY, a DNA repair enzyme, is unusual in that it binds exceedingly tightly to its products after the chemical steps of catalysis...
  80. ncbi IgH class switching and translocations use a robust non-classical end-joining pathway
    Catherine T Yan
    Howard Hughes Medical Institute, of Genetics, Boston, Massachusetts 02115, USA
    Nature 449:478-82. 2007
    ..CSR joins also display direct and microhomology joins, and CSR has been suggested to use C-NHEJ. Xrcc4 and DNA ligase IV (Lig4), which cooperatively catalyse the ligation step of C-NHEJ, are the most specific C-NHEJ factors; they ..
  81. doi Selective recognition of uracil and its derivatives using a DNA repair enzyme structural mimic
    Yu Lin Jiang
    Department of Chemistry, College of Arts and Sciences, East Tennessee State University, Johnson City, Tennessee 37614, USA
    J Org Chem 75:324-33. 2010
    ..Our study provides some insight into how uracil and its derivatives in DNA are bound by DNA repair enzymes through hydrogen bonding and pi-pi stacking interactions...
  82. ncbi cDNA and deduced amino acid sequence of a mouse DNA repair enzyme (APEX nuclease) with significant homology to Escherichia coli exonuclease III
    S Seki
    Department of Molecular Biology, Okayama University Medical School, Japan
    J Biol Chem 266:20797-802. 1991
    We purified a mouse DNA repair enzyme having apurinic/apyrimidinic endonuclease, DNA 3'-phosphatase, 3'-5'-exonuclease and DNA 3' repair diesterase activities, and designated the enzyme as APEX nuclease...
  83. pmc Expression levels of the DNA repair enzyme HAP1 do not correlate with the radiosensitivities of human or HAP1-transfected rat cell lines
    C J Herring
    CRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
    Br J Cancer 80:940-5. 1999
    ....
  84. pmc Efficient recognition of substrates and substrate analogs by the adenine glycosylase MutY requires the C-terminal domain
    N H Chmiel
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112 0850, USA
    Nucleic Acids Res 29:553-64. 2001
    The Escherichia coli DNA repair enzyme MutY plays an important role in the prevention of DNA mutations by removing misincorporated adenine residues from 7, 8-dihydro-8-oxo-2'-deoxyguanosine:2'-deoxyadenosine (OG:A) mispairs...
  85. ncbi cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues
    Y Tan
    Department of Molecular Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan
    Acta Med Okayama 50:53-60. 1996
    APEX nuclease is a mammalian DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3'-5'-exonuclease, DNA 3' repair diesterase and DNA 3'-phosphatase activities...
  86. ncbi Noncysteinyl coordination to the [4Fe-4S]2+ cluster of the DNA repair adenine glycosylase MutY introduced via site-directed mutagenesis. Structural characterization of an unusual histidinyl-coordinated cluster
    Troy E Messick
    Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 41:3931-42. 2002
    The Escherichia coli DNA repair enzyme MutY plays an important role in the recognition and repair of 7,8-dihydro-8-oxo-2'-deoxyguanosine-2'-deoxyadenosine (OG*A) mismatches in DNA...
  87. pmc DNA polymerase mu gene expression in B-cell non-Hodgkin's lymphomas: an analysis utilizing in situ hybridization
    April Chiu
    Department of Pathology, Weill Medical College of Cornell University, New York, New York 10021, USA
    Am J Pathol 161:1349-55. 2002
    DNA polymerase mu (pol mu) is a novel error-prone DNA repair enzyme bearing significant structural homology with terminal deoxynucleotidyltransferase...
  88. ncbi Progressive low-grade oligodendrogliomas: response to temozolomide and correlation between genetic profile and O6-methylguanine DNA methyltransferase protein expression
    Netta Levin
    Leslie and Michael Gaffin Center for Neuro Oncology, Hadassah Hebrew University Hospital, Jerusalem, Israel imbar
    Cancer 106:1759-65. 2006
    ..The DNA repair enzyme O6-methylguanine DNA methyltransferase (MGMT) may induce resistance to DNA-alkylating agents...
  89. ncbi Structure, promoter analysis and chromosomal assignment of the human APEX gene
    K Akiyama
    Department of Molecular Biology, Okayama University Medical School, Japan
    Biochim Biophys Acta 1219:15-25. 1994
    APEX nuclease is a mammalian DNA repair enzyme having apurinic/apyrimidinic endonuclease, 3'-5'-exonuclease, DNA 3' repair diesterase and DNA 3'-phosphatase activities...
  90. ncbi Contribution of a conserved phenylalanine residue to the activity of Escherichia coli uracil DNA glycosylase
    Ryan W Shaw
    Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610 0245, USA
    DNA Repair (Amst) 3:1273-83. 2004
    Uracil DNA glycosylase (UDG) excises uracil from DNA to initiate repair of this lesion. This important DNA repair enzyme is conserved in viruses, bacteria, and eukaryotes...
  91. ncbi The DNA repair enzyme, CPD-photolyase restores the infectivity of UV-damaged fowlpox virus isolated from infected scabs of chickens
    V Srinivasan
    Department of Veterinary Pathobiology, College of Veterinary Medicine, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, University of Illinois, Urbana, IL 61802 6178, USA
    Vet Microbiol 108:215-23. 2005
    ..of the virus are not completely known, we recently identified the presence of a virus-encoded novel DNA repair enzyme, CPD-photolyase, in FWPV...
  92. ncbi Mechanisms of DNA double strand break repair and chromosome aberration formation
    G Iliakis
    Institute of Medical Radiation Biology, University of Duisburg Essen Medical School, Essen, Germany
    Cytogenet Genome Res 104:14-20. 2004
    ..This component of DSB rejoining is severely compromised in cells with mutations in DNA-PKcs, Ku, DNA ligase IV, or XRCC4, as well as after chemical inhibition of DNA-PK, indicating that it reflects classical NHEJ; we ..
  93. pmc Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase
    A Hakura
    Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan
    J Bacteriol 173:3663-72. 1991
    ..which serves as a positive regulator of the adaptive response to alkylating agents and as a DNA repair enzyme. The gene which can make an ada-deficient strain of E...
  94. pmc A ubiquitin carrier protein from wheat germ is structurally and functionally similar to the yeast DNA repair enzyme encoded by RAD6
    M L Sullivan
    Department of Horticulture, University of Wisconsin Madison 53706
    Proc Natl Acad Sci U S A 86:9861-5. 1989
    ..Based on both the structural and enzymatic similarities to the RAD6 gene product, E2(23 kDa) may represent the first DNA repair enzyme identified in higher plants.
  95. pmc Uracil-DNA glycosylase-DNA substrate and product structures: conformational strain promotes catalytic efficiency by coupled stereoelectronic effects
    S S Parikh
    Skaggs Institute for Chemical Biology and the Department of Molecular Biology, MB 4, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 1027, USA
    Proc Natl Acad Sci U S A 97:5083-8. 2000
    Enzymatic transformations of macromolecular substrates such as DNA repair enzyme/DNA transformations are commonly interpreted primarily by active-site functional-group chemistry that ignores their extensive interfaces...
  96. pmc Fowlpox virus encodes a novel DNA repair enzyme, CPD-photolyase, that restores infectivity of UV light-damaged virus
    V Srinivasan
    Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61802, USA
    J Virol 75:1681-8. 2001
    ..Although expression of a homologous protein has been predicted for some chordopoxviruses, this report is the first to demonstrate that a poxvirus can utilize light to repair damage to its genome...
  97. pmc Heterogeneity of O6-alkylguanine DNA-alkyltransferase expression in human breast tumours
    M J Clemons
    Cancer Research UK Department of Medical Oncology, Christie Hospital, Wilmslow Road, Manchester M20 4BX, UK
    Br J Cancer 86:1797-802. 2002
    ..This is achieved by a specific DNA repair enzyme O6-alkylguanine DNA-alkyltransferase...
  98. pmc Mechanism of oxidative DNA damage in diabetes: tuberin inactivation and downregulation of DNA repair enzyme 8-oxo-7,8-dihydro-2'-deoxyguanosine-DNA glycosylase
    Simona Simone
    George O Brien Kidney Research Center, Department of Medicine, Division of Nephrology, University of Texas Health Science Center, San Antonio, Texas, USA
    Diabetes 57:2626-36. 2008
    ..To investigate potential mechanisms of oxidative DNA damage in a rat model of type 1 diabetes and in murine proximal tubular epithelial cells and primary culture of rat proximal tubular epithelial cells...
  99. ncbi Radiation-induced XRCC4 association with chromatin DNA analyzed by biochemical fractionation
    Radhika Pankaj Kamdar
    Research Laboratory for Nuclear Reactors and Department of Nuclear Engineering, Tokyo Institute of Technology, Tokyo, Japan
    J Radiat Res 51:303-13. 2010
    XRCC4, in association with DNA ligase IV, is thought to play a critical role in the ligation of two DNA ends in DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ) pathway...
  100. pmc Novel mechanism of regulation of the DNA repair enzyme OGG1 in tuberin-deficient cells
    Samy L Habib
    Department of Geriatrics, Geriatric Research, Education, and Clinical Center, South Texas Veterans Healthcare System, San Antonio, TX 78229, USA
    Carcinogenesis 31:2022-30. 2010
    ..protein encodes by tuberous sclerosis complex 2, Tsc2) deficiency is associated with the decrease in the DNA repair enzyme 8-oxoG-DNA glycosylase (OGG1) in tumour kidney of tuberous sclerosis complex (TSC) patients...
  101. ncbi Fpg protein of Escherichia coli is a zinc finger protein whose cysteine residues have a structural and/or functional role
    T R O'Connor
    Groupe Reparation des lesions Radio et Chimio Induites, Centre National de la Recherche Scientifique URA147 Institut National de la Santé et de la Recherche Médicale U140, Institut Gustave Roussy, Villejuif, France
    J Biol Chem 268:9063-70. 1993
    The Fpg protein of Escherichia coli is a DNA repair enzyme with DNA glycosylase, abasic site nicking, and deoxyribose excising activities...

Research Grants64

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS JOSEPH DELL'ITALIA; Fiscal Year: 2013
    ..cardiomyocyte that cleaved myofibrils (4-6) and nuclear matrix protein poly ADP-ribose polymerase (PARP)-a DNA repair enzyme in cell survival during I/R (7). However, the regulation of intracellular MMPs during I/R is not known...
  2. SAMY LEWIZ HABIB; Fiscal Year: 2016
    ..The DNA repair enzyme that recognizes and excises 8-oxodG is 8- oxoG-DNA glycosylase (OGG1)...
  3. Leona D Samson; Fiscal Year: 2016
    ..involved in protecting cells against alkylating agents) is that in certain cell types, expression of the Aag DNA repair enzyme, even at wild type levels, has the counter-intuitive consequence of rendering those cells extremely ..
  4. DNA Damage and Neurodegeneration in the Aging Brain
    Bruce A Yankner; Fiscal Year: 2010
    ..protein complex that contains the longevity gene Sirtl, the transcriptional co-repressor NCOR1, and the DNA repair enzyme hOGG1. Our preliminary studies also raise the possibility that age-related ...
  5. Sandeep Burma; Fiscal Year: 2015
    ..upon our preliminary results, we hypothesize that cross talk between the EGFRvIII-PI3K-Akt axis and the DNA repair enzyme, DNA-PKcs, underlies the radioresistance of GBMs and that this connection can be targeted for effective ..
  6. Catherine T Yan; Fiscal Year: 2016
    ....
  7. Impact of genetic variation on response to GO therapy in COG-AML clinical trials
    ROLAND BRUNO WALTER; Fiscal Year: 2013
    ..Moreover, this research will provide novel insights into the impact of genetic polymorphisms on efficacy of standard therapeutics with broad implications for the treatment of AML. ..
  8. Alan E Tomkinson; Fiscal Year: 2014
    ..In this proposal, we will focus on delineating the molecular mechanisms of DNA ligase IV-dependent NHEJ...
  9. The Roles of Trm9 and tRNA Methylation in the DNA Damage Response
    Thomas J Begley; Fiscal Year: 2010
    ..activity to the regulation of specific DNA repair proteins (Rad54, Brca2, Bccip, Ercc8, Tdg, Msh4, Rad51, and DNA Ligase IV), and we will study how Trm9 affects their damage-induced translation...
  10. SHEILA SUE DAVID; Fiscal Year: 2016
    ..HEALTH RELEVANCE: This project will provide information on the functional consequences of variations in the DNA repair enzyme MUTYH that are correlated with the inherited colorectal cancer syndrome known as MUTYH-associated polyposis (..
  11. Jann N Sarkaria; Fiscal Year: 2016
    ..Whole exome-seq and RNA-seq in TMZ-resistant models without MGMT elevation identified mutations in DNA ligase IV, Ku70 and Ku80, which are key components in the non-homologous end-joining (NHEJ) DNA repair pathway...
  12. TRANSPOSASES IN ETOPOSIDE RESISTANCE
    Robert A Hromas; Fiscal Year: 2012
    ..It was found to interact with DNA Ligase IV, consistent with its NHEJ repair activity. Metnase also was an endonuclease preferential for supercoiled DNA...
  13. Alan E Tomkinson; Fiscal Year: 2016
    ..abstract_text> ..
  14. A Hybrid PARP Inhibitor and Redox Catalyst for Lung Transplantation
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..species induce DNA single strand breaks that activate poly(ADP-ribose) polymerase (PARP), a nuclear DNA repair enzyme that in turn depletes its substrate NAD, resulting in an exhaustion of intracellular energetics, ATP ..
  15. A Redox-active PARP Inhibitor for Lung Transplantation
    ANDREW LURIE SALZMAN; Fiscal Year: 2012
    ..and nitrosative species that in turn induce DNA single strand breakage and triggering of the nuclear DNA repair enzyme, poly(ADP-ribose) polymerase-1 (PARP-1)...
  16. Sarah Delaney; Fiscal Year: 2014
    ..Furthermore, the DNA repair enzyme OGG1, which is known to remove 8-oxoG from DNA duplexes, has also been linked to dynamic mutations...
  17. A Thioredoxin Mimetic for Radiocontrast Nephropathy
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..When this genomic injury is then recognized by the nuclear DNA repair enzyme poly (ADP-ribose) polymerase ("PARP"), NAD is cleaved to generate the product poly(ADP-ribose)...
  18. PARP inhibitor and Redox Catalyst Conjugate for Traumatic Brain Injury
    Kanneganti Murthy; Fiscal Year: 2012
    ..R-503 is both 1) a potent inhibitor of the nuclear DNA repair enzyme poly(ADP-ribose) polymeras ("PARP") (IC50=20 nM), and 2) a dihydrolipoate ("DHL")-based redox catalyst that ..
  19. Resuscitation of smoke inhalation and burn injury with a thioredoxin mimetic
    ANDREW LURIE SALZMAN; Fiscal Year: 2012
    ..When this genomic injury is then recognized by the nuclear DNA repair enzyme poly(ADP-ribose) polymerase ("PARP"), NAD is cleaved to generate the product poly(ADP-ribose)...
  20. PARP Inhibitor and Redox Catalyst for Ventilatory Trauma
    Prakash Jagtap; Fiscal Year: 2013
    ..generation of peroxynitrite via broad-spectrum redox catalysis, and 2) competitively inhibits the nuclear DNA repair enzyme poly(ADP-ribose) polymerase 1 ("PARP- 1")...
  21. Neonatal Chemoselection Following Ex Vivo Gene Transfer For Hereditary Disorders
    KARIN L GAENSLER; Fiscal Year: 2012
    ..This variant DNA repair enzyme confers resistance to benzylguanine, an inhibitor of endogenous MGMT, and to chloroethylating agents such as ..
  22. Role of ABH1's AP endonuclease activity in immunoglobulin gene diversification
    TINA A MUELLER; Fiscal Year: 2010
    ..The focus of this proposal is to determine whether ABH1 (mammalian homologue I of the Escherichia coli AlkB DNA repair enzyme) functions in lymphocytes to cleave abasic sites and generate breaks that are the requisite intermediate for ..
  23. Peter A Beal; Fiscal Year: 2016
    ..In addition, we will define the basis for ADAR1 editing of the mRNA for the DNA repair enzyme NEIL1...
  24. W M BUJALOWSKI; Fiscal Year: 2016
    ..The profound and fundamental difference between the replicative and repair polymerases is that the DNA repair enzyme must recognize a specific structure of the damaged DNA prior to the catalysis, in the context of ..
  25. Raymond A Swanson; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): The DNA repair enzyme, PARP-1, has emerged as a critical determinant of neuronal death in brain ischemia and other conditions...
  26. Mycobacterium tuberculosis dUTPase as a tool in disease control
    Judit Toth; Fiscal Year: 2012
    ..PUBLIC HEALTH RELEVANCE The present proposal focuses on an important DNA repair enzyme called dUTPase of the bacterium causing tuberculosis...
  27. Robert A Hromas; Fiscal Year: 2014
    ..Metnase was found to interact with the NBS1, an early NHEJ repair pathway component, DNA Ligase IV, a final component of the NHEJ pathway, and Pso4, an uncharacterized DSB repair component...
  28. Repair of Clustered DNA Damages
    Yoke W Kow; Fiscal Year: 2010
    ..we will use siRNA to knockdown various DNA repair genes in both HaCat and PIG1 cells and determine which DNA repair enzyme plays a role in the generation of the unique V599 mutation...
  29. GENETIC EPIDEMIOLOGY OF LUNG CANCER
    Ann Schwartz; Fiscal Year: 2009
    ..in studies of candidate susceptibility genes focused on phase I and phase II enzyme polymorphisms and DNA repair enzyme polymorphisms...
  30. HIV HOST-CELL INTERACTIONS
    Michael Emerman; Fiscal Year: 2010
    ..In addition, Vpr interacts with a host cell DNA repair enzyme called Uracil DNA Glycosidase (UNG)...
  31. Workshop on DNA Repair and related DNA transactions
    James Ford; Fiscal Year: 2001
    ..The session topics include: DNA repair enzyme structure, Recognition of damaged DNA and initiation of global genomic repair, Chromatin structure and ..
  32. GORDON CONFERENCE ON MAMMALIAN DNA REPAIR, 1999
    Philip Hanawalt; Fiscal Year: 1999
    ..Repair of endogenous damage, in nuclei and mitochondria; Translesion synthesis and repair of mismatches; DNA repair enzyme structure and substrate interactions; Nucleotide excision repair- role of transcription; Base excision ..
  33. Identification of radiation sensitivity alleles by whole exome sequencing
    Patrick Concannon; Fiscal Year: 2012
    ....
  34. Michael R Lieber; Fiscal Year: 2015
    ..for the bias toward deletion rather than inversion in D to J joining;identified the ligase complex (XRCC4:DNA ligase IV) for the final step of V(D)J recombination using both biochemistry and genetics;created a human cell line ..
  35. DNA Recombination/Repair Mechanisms in Memory
    SANDRA PENA DE ORTIZ; Fiscal Year: 2011
    ..the function of putative recombination effector enzymes, Flap Structure-Specific Endonuclease-1 (FEN-1) and DNA ligase IV. AIM # 2 examines amygdalar genomic rearrangement of putative DNA recombination target genes, such as ..
  36. Mechanism and regulation of V(D)J repair
    Patricia Cortes; Fiscal Year: 2013
    ..Information gained from the proposed studies will contribute to a molecular and structural understanding of NHEJ, V(D)J recombination and human diseases linked to defects in both of these processes. ..
  37. Systematic Genetic Analysis of Yeast NHEJ
    Thomas Wilson; Fiscal Year: 2009
    ..These include: (i) the structural end- binding protein Ku;(ii) DNA ligase IV, comprised of its catalytic subunit (Lig4/Dnl4) and two supporting proteins (XRCC4/Lif1 and XLF/Nej1);and (iii)..
  38. THOMAS EDWARD WILSON; Fiscal Year: 2014
    ..These include: (i) the structural end- binding protein Ku;(ii) DNA ligase IV, comprised of its catalytic subunit (Lig4/Dnl4) and two supporting proteins (XRCC4/Lif1 and XLF/Nej1);and (iii)..
  39. Xanthine DNA Glycosylase in Mammalian Systems
    Weiguo Cao; Fiscal Year: 2010
    ..The short-term goal of this proposal is to identify this previously unknown DNA repair enzyme involved in removal of xanthine base damage in mammalian systems...
  40. FUNCTION FOR VPR AND VPX OF PRIMATE LENTIVIRUSES
    Mario Stevenson; Fiscal Year: 2009
    ..causes a delay in the G2 phase of the cell cycle and has additionally been shown to associate with the DNA repair enzyme, uracil DNA glycosylase...
  41. OVERCOMING ALKYLTRANSFERASE-RELATED DRUG RESISTANCE
    TIMOTHY SPIRO; Fiscal Year: 2000
    ..These adducts are repaired by the DNA repair enzyme 06-alkylguanine DNA alkyltransferase (AGT), in a reaction that irreversibly inactivates the enzyme...
  42. POST-TRANSLATIONAL MECHANISMS IN URACIL-DNA REPAIR
    SALVATORE CARADONNA; Fiscal Year: 2004
    ..Uracil-DNA glycosylase (UDG) is a critical DNA repair enzyme that functions in the correction of transition mutations caused by cytosine deamination...
  43. Mechanism of Base-Flipping by DNA Modifying Enzymes
    RICHARD BUNT; Fiscal Year: 2003
    ..as a tool to probe the enzymatic mechanism of base flipping by Uracil DNA Glycosylase (UDG),a prototypical DNA repair enzyme. The synthesis of 5'-methyl nucleoside analogs of defined stereochemistry should hinder the bond rotations ..
  44. G/A MISMATCH RECOGNITION AND REPAIR BY E COLI MUTY
    SHEILA DAVID; Fiscal Year: 1999
    ..aspect of MutY is that it is a [4Fe-4S] cluster-containing protein and has significant homology to the DNA repair enzyme, endonuclease III...
  45. Suk Hee Lee; Fiscal Year: 2015
    ..Finally, Metnase mutants lacking its biochemical activities will be examined for promotion of chromosome decatenation activity. ..
  46. Primary Immuno-Deficiencies Affecting Specific Stages of the Immune Response
    CORNELIS P TERHORST; Fiscal Year: 2013
    ..altered mice, this application seeks to define how mutations in the SH2D1A, SH2D1B/C, TACI, RAG-1/2, DNA Ligase IV or Cernunnos genes affect T and B cell development and T cell dependent and/or T cell-independent ..
  47. ENDONUCLEASE V EXPRESSION IN HUMAN CELLS
    Earl Henderson; Fiscal Year: 1992
    ..photoproducts on DNA synthesis, transcription, survival, repair and mutations in human cells; and affords as the opportunity to examine the function of a well characterized DNA repair enzyme in normal and repair- deficient human cells.
  48. Generation and Repair of an Unusual UV Photoproduct
    Joan Broderick; Fiscal Year: 2006
    ..high resistance of bacterial spores to UV irradiation, and this resistance arises in part from the novel DNA repair enzyme that repairs SP...
  49. REPAIR OF OXIDATIVE AND RADIATION-INDUCED DNA DAMAGE
    PAUL DOETSCH; Fiscal Year: 1993
    ..The goal of this proposal is to gain an understanding of the role mediated by redoxyendonuclease, a DNA repair enzyme that recognizes a variety of base damage products, in the eukaryotic cellular response to oxidative and ..
  50. FUNCTION OF THE S CEREVISIAE PHR1 GENE AND PHOTOLYASE
    GWENDOLYN SANCAR; Fiscal Year: 2004
    ..cerevisiae. The proposal focuses on the PHR1 gene, which encodes the DNA repair enzyme photolyase...
  51. IDENTIFICATION OF COMPONENTS OF THE CELL DEATH PATHWAY
    Claudius Vincenz; Fiscal Year: 2000
    ..investigator has recently cloned a CED-3/ICE-related gene, designated YAMA, that specifically cleaves the DNA repair enzyme poly (ADP-ribose) polymerase (PARP) from its native 116 kDa form a characteristic 85 kDa fragment that is ..
  52. Multicenter Trial of O6BG and Topical BCNU for CTCL
    Kevin Cooper; Fiscal Year: 2007
    ..on O6-benzylguanine (O6BG), which irreversibly inactivates O6-alkylguanine DNA alkyltransferase (AGT), a DNA repair enzyme encoded by the gene MGMT that is responsible for nitrosourea resistance in many malignancies, including CTCL...
  53. REGULATION OF BETA-POLYMERASE IN PREMALIGNANT CONDITIONS
    Craig Tendler; Fiscal Year: 1992
    We will investigate the regulation and expression of the DNA repair enzyme, beta-polymerase, in clinical conditions complicated by a high incidence of malignancy and in human tumors which have become resistant to chemotherapeutic agents...
  54. TRANSGENIC MODELS FOR NEUROTOXICITY AND DNA REPAIR
    MELVIN BILLINGSLEY; Fiscal Year: 1999
    ..In the third project normal and mutated forms of the important DNA repair enzyme 06-alkylguanine-DAN alkyltransferase (AGT) will be expressed in the mouse lung using promoters specific for ..
  55. RADIATION-INDUCED DNA DAMAGE AND REPAIR IN EUKARYOTES
    PAUL DOETSCH; Fiscal Year: 1993
    ..candidate's research proposal is to gain an understanding of the role mediate by redoxyendonuclease (RE), a DNA repair enzyme that recognizes a variety of base damage products, in the eukaryotic cellular response to oxidative and ..
  56. Molecular Mechanisms of Lymphomagenesis
    Kevin Mills; Fiscal Year: 2009
    ..These studies will therefore lay a foundation for the development of improved diagnostic methods, as well as the formulation and clinical development of new, possibly individualized, therapeutic approaches. ..
  57. GENETIC VARIATION IN HUMAN NHEJ DNA REPAIR GENES
    Carl Anderson; Fiscal Year: 2005
    ..the three components of DNA-PK (DNA-PKcs, Ku70, Ku80), the double-stranded DNA activated protein kinase, and DNA ligase IV and XRCC4. Additional NHEJ components (e.g...
  58. KARP 1 V(D)J RECOMBINATION AND DNA REPAIR
    ERIC HENDRICKSON; Fiscal Year: 2001
    ..In particular, the DNA-dependent protein kinase catalytic subunit, Ku86, Ku70, XR-1 and DNA ligase IV gene products all appear to be required for both of these processes...
  59. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    BRENDA SALERNO; Fiscal Year: 2006
    ..NHEJ requires the DNA Ligase IV/XRCC4 complex and the DNA-dependent Protein Kinase (DNA-PK), which is formed by DNA-PKcs, and the heterodimer ..
  60. DRUG RESISTANCE AND DNA REPAIR IN BRAIN TUMORS
    Mitchel Berger; Fiscal Year: 1992
    ..approach: Specific Aim 1 -- to test the hypothesis that BCNU resistance is related to the activity of the DNA repair enzyme. Alkyltransferase which removes DNA drug adducts, and, Specific Aim 2 -- to test the hypothesis that BCNU ..
  61. CELL BIOLOGY OF OXIDATIVE DNA DAMAGE AND REPAIR
    Miguel Berrios; Fiscal Year: 2004
    ..8-Oxoguanine has been shown to be mutagenic in vivo and in vitro. Recently, mogg1, a murine 8- oxoguanine-DNA repair enzyme was cloned and over expressed in transgenic animals...
  62. Gene Regulation and DNA Damage in the Aging Brain
    Bruce Yankner; Fiscal Year: 2009
    ..the transcriptional co-repressor NCOR1, the human ortholog of the yeast longevity gene SIR2 (Sirt1), and the DNA repair enzyme hOGG1...
  63. Protein alterations in skeletal muscle cells with improved mtDNA repair
    Lyudmila I Rachek; Fiscal Year: 2010
    ..To complete this study we propose to make use of a new liquid chromatography tandem mass spectrometry (LC-MS/MS) approach. ..
  64. Aging of the Nigrostriatal System: Role of DNA Repair
    Fernando Cardozo Pelaez; Fiscal Year: 2004
    ..Wild type and knockout mice will be challenged with the neurotoxin MPTP in order to detect changes in the dopaminergic neurons susceptibility as a consequence of aging and lack of DNA repair ..