DGKE

Summary

Gene Symbol: DGKE
Description: diacylglycerol kinase epsilon
Alias: AHUS7, DAGK5, DAGK6, DGK, NPHS7, diacylglycerol kinase epsilon, DAG kinase epsilon, DGK-epsilon, diacylglycerol kinase, epsilon 64kDa, diglyceride kinase epsilon
Species: human

Top Publications

  1. ncbi Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates
    W Tang
    Program in Human Molecular Biology and Genetics, The Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, Utah 84112
    J Biol Chem 271:10237-41. 1996
  2. pmc The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    Biochem J 318:583-90. 1996
  3. ncbi Phospholipase C/diacylglycerol kinase-mediated signalling is required for benzothiadiazole-induced oxidative burst and hypersensitive cell death in rice suspension-cultured cells
    Jie Chen
    Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang
    Protoplasma 230:13-21. 2007
  4. doi Phosphatidic acid regulates signal output by G protein coupled receptors through direct interaction with phospholipase C-beta(1)
    Irene Litosch
    Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA
    Cell Signal 21:1379-84. 2009
  5. ncbi Cloning and characterization of diacylglycerol kinase iota splice variants in rat brain
    Tsukasa Ito
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Japan
    J Biol Chem 279:23317-26. 2004
  6. pmc EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes
    K Yamada
    Department of Liberal Arts and Sciences, School of Health Sciences, Sapporo Medical University, Japan
    Biochem J 321:59-64. 1997
  7. ncbi The pattern of deoxycytidine- and deoxyguanosine kinase activity in relation to messenger RNA expression in blood cells from untreated patients with B-cell chronic lymphocytic leukemia
    Kourosh Lotfi
    Department of Medicine and Care, Clinical Pharmacology, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Biochem Pharmacol 71:882-90. 2006
  8. ncbi The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase
    Elizabeth H Clark
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 42:11065-73. 2003
  9. pmc Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo
    Antony M Jose
    Department of Molecular, Cellular, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:2730-6. 2005
  10. pmc Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats
    I Masai
    Molecular Genetics Research Laboratory, Faculty of Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 90:11157-61. 1993

Research Grants

  1. XIAOPING ZHONG; Fiscal Year: 2016
  2. Matthew J Riese; Fiscal Year: 2014
  3. Daniel M Raben; Fiscal Year: 2016
  4. DGK in T Cell Regulation and Tolerance
    Thomas F Gajewski; Fiscal Year: 2010
  5. CRYSTALLIZATION OF DIACYLGLYCEROL KINASE
    JAMES BOWIE; Fiscal Year: 2000
  6. Thomas F Gajewski; Fiscal Year: 2016
  7. CLONING / CHARACTERIZATION OF NOVEL DIACYLGLYCEROL KINAS
    Susan Jaken; Fiscal Year: 1999
  8. ENZYMOLOGY OF GOLGI STACK FORMATION
    Vivek Malhotra; Fiscal Year: 2007
  9. Molecular Dissection of T Cell Anergy
    Thomas Gajewski; Fiscal Year: 2005
  10. John K Buolamwini; Fiscal Year: 2015

Detail Information

Publications184 found, 100 shown here

  1. ncbi Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates
    W Tang
    Program in Human Molecular Biology and Genetics, The Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, Utah 84112
    J Biol Chem 271:10237-41. 1996
    ..This enzyme, which we designated DGK epsilon, has two distinctive zinc finger-like structures in its N-terminal region, but does not contain the E-F hand ..
  2. pmc The C-terminal part of diacylglycerol kinase alpha lacking zinc fingers serves as a catalytic domain
    F Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    Biochem J 318:583-90. 1996
    All mammalian diacylglycerol kinase (DGK) isoenzymes so far cloned consist of four conserved regions, namely, C1, C2 (tandem EF-hand structures), C3 (tandem cysteine-rich zinc finger sequences) and the C-terminal C4 domains...
  3. ncbi Phospholipase C/diacylglycerol kinase-mediated signalling is required for benzothiadiazole-induced oxidative burst and hypersensitive cell death in rice suspension-cultured cells
    Jie Chen
    Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang
    Protoplasma 230:13-21. 2007
    The involvement of phospholipase C/diacylglycerol kinase (PLC/DGK)-mediated signalling in oxidative burst and hypersensitive cell death was studied in rice suspension-cultured cells treated with benzothiadiazole (BTH) and infected by ..
  4. doi Phosphatidic acid regulates signal output by G protein coupled receptors through direct interaction with phospholipase C-beta(1)
    Irene Litosch
    Department of Molecular and Cellular Pharmacology, Miller School of Medicine, University of Miami, Miami, Florida 33101, USA
    Cell Signal 21:1379-84. 2009
    ..generated downstream of monomeric Rho GTPases via phospholipase D (PLD) and additionally by diacylglycerol kinases (DGK), both stimulates phospholipase C-beta(1) (PLC-beta(1)) and potentiates stimulation of PLC-beta(1) activity by ..
  5. ncbi Cloning and characterization of diacylglycerol kinase iota splice variants in rat brain
    Tsukasa Ito
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Japan
    J Biol Chem 279:23317-26. 2004
    Diacylglycerol kinase (DGK) catalyzes phosphorylation of a second messenger diacylglycerol (DG) to phosphatidic acid in cellular signal transduction...
  6. pmc EF-hand motifs of alpha, beta and gamma isoforms of diacylglycerol kinase bind calcium with different affinities and conformational changes
    K Yamada
    Department of Liberal Arts and Sciences, School of Health Sciences, Sapporo Medical University, Japan
    Biochem J 321:59-64. 1997
    The three diacylglycerol kinase isoenzymes (DGK alpha, DGK beta and DGK gamma) cloned so far contain in common a tandem repeat of EF-hand motifs...
  7. ncbi The pattern of deoxycytidine- and deoxyguanosine kinase activity in relation to messenger RNA expression in blood cells from untreated patients with B-cell chronic lymphocytic leukemia
    Kourosh Lotfi
    Department of Medicine and Care, Clinical Pharmacology, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
    Biochem Pharmacol 71:882-90. 2006
    Deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK) catalyze the first step in the intracellular cascade of fludarabine (2-fluoroadenine-beta-D-arabinofuranoside) and cladribine (2-chlorodeoxyadenosine) phosphorylation, which ..
  8. ncbi The role of tryptophan residues in an integral membrane protein: diacylglycerol kinase
    Elizabeth H Clark
    Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Southampton SO16 7PX, United Kingdom
    Biochemistry 42:11065-73. 2003
    ..We have studied the effect of mutating the five Trp residues in the diacylglycerol kinase (DGK) of Escherichia coli to Leu residues...
  9. pmc Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivo
    Antony M Jose
    Department of Molecular, Cellular, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 280:2730-6. 2005
    Diacylglycerol kinases (DGKs) inhibit diacylglycerol (DAG) signaling by phosphorylating DAG. DGK-1, the Caenorhabditis elegans ortholog of human neuronal DGK, inhibits neurotransmission to control behavior...
  10. pmc Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats
    I Masai
    Molecular Genetics Research Laboratory, Faculty of Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 90:11157-61. 1993
    ..Biochemically, the rdgA mutant lacks an eye-specific and membrane-associated diacylglycerol kinase (DGK; EC 2.7.1.107) activity in a gene-dosage-dependent manner, suggesting that rdgA gene encodes a DGK...
  11. pmc Regulation of neurite outgrowth in N1E-115 cells through PDZ-mediated recruitment of diacylglycerol kinase zeta
    Yury Yakubchyk
    Department of Cellular and Molecular Medicine, University of Ottawa, Ontario, Canada
    Mol Cell Biol 25:7289-302. 2005
    Syntrophins are scaffold proteins that regulate the subcellular localization of diacylglycerol kinase zeta (DGK-zeta), an enzyme that phosphorylates the lipid second-messenger diacylglycerol to yield phosphatidic acid...
  12. pmc The cysteine-rich domain of human proteins, neuronal chimaerin, protein kinase C and diacylglycerol kinase binds zinc. Evidence for the involvement of a zinc-dependent structure in phorbol ester binding
    S Ahmed
    Institute of Molecular and Cell Biology, National University of Singapore
    Biochem J 280:233-41. 1991
    ..DG is produced transiently as it is rapidly metabolized by DG kinase (DGK) to phosphatidic acid...
  13. pmc A 104-kDa diacylglycerol kinase containing ankyrin-like repeats localizes in the cell nucleus
    K Goto
    Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan
    Proc Natl Acad Sci U S A 93:11196-201. 1996
    ..This cDNA encoded a 929-aa, 104-kDa polypeptide termed DGK-IV...
  14. ncbi Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway
    Ana Ramos-Díaz
    Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología Molecular de Plantas, Calle 43, 130 Col Chuburná de Hidalgo, Merida Yucatan, Mexico
    Planta 225:393-401. 2007
    ..1.4.4) or via Phospholipase C (PLC; EC: 3.1.4.3), and diacylglycerol kinase (DGK; EC 2.7.1.107)...
  15. doi Diacylglycerol kinase delta associates with receptor for activated C kinase 1, RACK1
    Shin ichi Imai
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo, 060 8556, Japan
    Biochim Biophys Acta 1791:246-53. 2009
    The delta-isozyme (type II) of diacylglycerol kinase (DGK) is known to positively regulate growth factor receptor signaling...
  16. pmc Molecular species of phosphatidylinositol-cycle intermediates in the endoplasmic reticulum and plasma membrane
    Yulia V Shulga
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 49:312-7. 2010
    ..by comparing the compositions of the two membranes isolated from embryonic fibroblasts obtained from diacylglycerol kinase epsilon (DGKepsilon) knockout (KO) and wild-type (WT) mice...
  17. doi Diacylglycerol kinase beta accumulates on the perisynaptic site of medium spiny neurons in the striatum
    Yasukazu Hozumi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Neurosci 28:2409-22. 2008
    ..The former activates protein kinase C and the latter mobilizes Ca(2+) from intracellular store. DAG kinase (DGK) then phosphorylates DAG to produce another second messenger (phosphatidic acid)...
  18. ncbi Nod factor-induced phosphatidic acid and diacylglycerol pyrophosphate formation: a role for phospholipase C and D in root hair deformation
    M den Hartog
    Swammerdam Institute for Life Sciences, Department of Plant Physiology, University of Amsterdam, Kruislaan 318, NL 1098 SM, Amsterdam, The Netherlands
    Plant J 25:55-65. 2001
    ..PA) and diacylglycerol pyrophosphate, indicative of PLD or PLC activity in combination with diacylglycerol kinase (DGK) and PA kinase. Treatment with Nod factor had similar effects, although less pronounced...
  19. ncbi Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26
    G T Ma
    Department of Biochemistry, Ohio State University, Columbus 43210 1292
    J Biol Chem 270:6595-601. 1995
    ..kinases, deoxycytidine kinase/deoxyadenosine kinase (dCK/dAK) and deoxyguanosine kinase/deoxyadenosine kinase (dGK/dAK) are required, together with thymidine kinase (TK), for deoxynucleotide synthesis in Lactobacillus acidophilus ..
  20. ncbi Low-intensity exercise increases skeletal muscle protein expression of PPARdelta and UCP3 in type 2 diabetic patients
    Tomas Fritz
    Centre of Family Medicine, Karolinska University Hospital, Karolinska Institutet, 171 11 Stockholm, Sweden
    Diabetes Metab Res Rev 22:492-8. 2006
    ..We tested the hypothesis that changes in expression of key genes in skeletal muscles relate to exercise-induced improvements in type 2 diabetic patients...
  21. ncbi Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificity
    Martin Welin
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, Biomedical Center, S 751 24 Uppsala, Sweden
    J Mol Biol 366:1615-23. 2007
    ..Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known...
  22. ncbi [RasGRP proteins--Ras-activating factors]
    Mateusz Szamałek
    Katedra Biochemii Farmaceutycznej, Uniwersytet Medyczny im K Marcinkowskiego, Poznan
    Postepy Biochem 53:112-20. 2007
    ..signal transmitted by RasGRP is terminated as a result of DAG phosphorylation catalyzed by diacylglycerol kinase (DGK)...
  23. ncbi Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin-binding isoform
    W A Snedden
    Department of Botany, University of Toronto, Toronto, Ontario, Canada, M5S 3B2
    Plant J 24:317-26. 2000
    ..Both recombinant LeCBDGK and LeDGK1 were catalytically active in vitro. Anti-DGK antiserum detected two immunoreactive proteins associated with microsomal and plasma membrane fractions from cell ..
  24. ncbi Nuclear localization of diacylglycerol kinase zeta in neurons
    Yasukazu Hozumi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Neurosci 18:1448-57. 2003
    Diacylglycerol kinase (DGK) is involved in intracellular signal transduction as a regulator of levels of diacylglycerol which leads to protein kinase C activation...
  25. ncbi Morphological changes and spatial regulation of diacylglycerol kinase-zeta, syntrophins, and Rac1 during myoblast fusion
    Hanan Abramovici
    Department of Cellular and Molecular Medicine, Center for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada
    Cell Motil Cytoskeleton 64:549-67. 2007
    ..Our recent studies suggest diacylglycerol kinase-zeta (DGK-zeta), an enzyme that metabolizes diacylglycerol to yield phosphatidic acid, plays an important role in actin ..
  26. doi Kinase activity of the dgk gene product is involved in the virulence of Streptococcus mutans
    Yukie Shibata
    Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Microbiology 155:557-65. 2009
    C-terminal deletion of the diacylglycerol kinase (Dgk) homologue of the cariogenic oral bacterium Streptococcus mutans resulted in loss of aciduricity...
  27. doi Localization of diacylglycerol kinase epsilon on stress fibers in vascular smooth muscle cells
    Tomoyuki Nakano
    Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Mukogawa cho 1 1, Nishinomiya, Hyogo, 663 8501, Japan
    Cell Tissue Res 337:167-75. 2009
    The expression pattern of diacylglycerol kinase (DGK) and the biological significance of DGKepsilon in vascular smooth muscle cells were investigated...
  28. ncbi AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression
    Fernando C Gómez-Merino
    University of Potsdam, Institute of Biochemistry and Biology, Karl Liebknecht Strasse 24 25, Haus 20, D 14476 Golm Potsdam, Germany
    J Biol Chem 279:8230-41. 2004
    Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Both DAG and PA are implicated in signal transduction pathways...
  29. ncbi Measurement of mammalian diacylglycerol kinase activity in vitro and in cells
    Richard M Epand
    Department of Biochemistry and Biomedical Sciences, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada
    Methods Enzymol 434:293-304. 2007
    Diacylglycerol kinase (DGK) catalyzes the conversion of diacylglycerol to phosphatidic acid. Because both the lipid substrate and the product are important in regulation, this enzyme plays an important role in signal transduction...
  30. pmc Dramatic differences in the roles in lipid metabolism of two isoforms of diacylglycerol kinase
    Stephen B Milne
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Biochemistry 47:9372-9. 2008
    ....
  31. doi Determination of the topology of the hydrophobic segment of mammalian diacylglycerol kinase epsilon in a cell membrane and its relationship to predictions from modeling
    Marc Decaffmeyer
    Faculte Universitaire des Sciences Agronomiques de Gembloux, Centre de Biophysique Moleculaire Numerique, Passage des Deportes, 2, 5030 Gembloux, Belgium
    J Mol Biol 383:797-809. 2008
    ..The work also demonstrates that the wild-type DGKepsilon is a monotopic protein...
  32. pmc Diacylglycerol kinase zeta regulates Ras activation by a novel mechanism
    M K Topham
    The Huntsman Cancer Institute and Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    J Cell Biol 152:1135-43. 2001
    ..b>DGK zeta, but not other DGKs, completely eliminated Ras activation induced by RasGRP, and DGK activity was required for ..
  33. ncbi A traditional herbal medicine, rikkunshi-to (TJ-43), prevents intracellular signaling disorders in gastric smooth muscle of diabetic rats
    Yasushi Sakai
    Division of Physiology, Department of Occupational Therapy, School of Nursing and Rehabilitation Sciences, Showa University, Yokohama 226, Japan
    Am J Chin Med 32:245-56. 2004
    ..The present study demonstrated that diacylglycerol kinase (DGK) activity in diabetic gastric smooth muscle in the resting state was approximately 3...
  34. ncbi Cardiac-specific overexpression of diacylglycerol kinase zeta attenuates left ventricular remodeling and improves survival after myocardial infarction
    Takeshi Niizeki
    Department of Cardiology, Yamagata University School of Medicine, 2 2 2 Iida nishi, Yamagata, Japan 990 9585
    Am J Physiol Heart Circ Physiol 292:H1105-12. 2007
    ..DAG kinase (DGK) phosphorylates DAG and controls cellular DAG levels, thus acting as a negative regulator of PKC and subsequent ..
  35. ncbi Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosides
    L Wang
    Department of Veterinary Medical Chemistry, The Swedish University of Agricultural Sciences, The Biomedical Centre, SE 751 23 Uppsala, Sweden
    Mol Microbiol 42:1065-73. 2001
    ..High levels of thymidine kinase (TK), deoxycytidine kinase (dCK), deoxyguanosine kinase (dGK) and deoxyadenosine kinase (dAK) activities were detected in extracts from Mycoplasma pneumoniae, Mycoplasma ..
  36. ncbi Diacylglycerol kinase-theta is localized in the speckle domains of the nucleus
    Giovanna Tabellini
    Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell Apparato Locomotore, Sezione di Anatomia Umana, Cell Signalling Laboratory, Universita di Bologna, 40126, Bologna, Italy
    Exp Cell Res 287:143-54. 2003
    ..Here, we show, by immunofluorescence staining and confocal analysis, that DGK-theta localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12, and HeLa...
  37. ncbi The alpha isoform of diacylglycerol kinase exhibits arachidonoyl specificity with alkylacylglycerol
    Richard M Epand
    Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario L8N 3Z5, Canada
    Biochemistry 43:14778-83. 2004
    We compared the diacylglycerol kinase (DGK) catalyzed phosphorylation of 1-O-hexanoyl-2-oleoylglycerol (HOG) with 1-O-hexanoyl-2-arachidonoylglycerol (HAG)...
  38. ncbi Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy
    Hirokazu Yahagi
    Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1 1 Seiryo machi, Aoba ku, Sendai 980 8574, Japan
    Biochem Biophys Res Commun 332:101-8. 2005
    To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation...
  39. ncbi Epileptogenesis in diacylglycerol kinase epsilon deficiency up-regulates COX-2 and tyrosine hydroxylase in hippocampus
    Walter J Lukiw
    Neuroscience Center of Excellence, Louisiana State University Health Sciences Center, School of Medicine in New Orleans, LA 70112, USA
    Biochem Biophys Res Commun 338:77-81. 2005
    ..Of the nine mammalian DGK isotypes known, DGK epsilon (DGKepsilon) shows specificity for arachidonoyldiacylglycerol (20:4-DAG) and selectively contributes to ..
  40. ncbi Pharmacological evidence for the involvement of diacylglycerol lipase in depolarization-induced endocanabinoid release
    Yuki Hashimotodani
    Department of Neurophysiology, Graduate School of Medicine, Osaka University, Suita 565 0871, Japan
    Neuropharmacology 54:58-67. 2008
    ..The chemicals we tested include inhibitors for phospholipase C (U73122 and ET-18), diacylglycerol kinase (DGK inhibitor 1), phosphatidic acid phosphohydrolase (propranolol), and diacylglycerol lipase (DGL; RHC-80267 and ..
  41. ncbi Anabolism of amdoxovir: phosphorylation of dioxolane guanosine and its 5'-phosphates by mammalian phosphotransferases
    Joy Y Feng
    Gilead Sciences, 4 University Place, 4611 University Drive, Durham, NC 27707, USA
    Biochem Pharmacol 68:1879-88. 2004
    ..DXG using 15 purified enzymes from human (8), animal (6), and yeast (1) sources, including deoxyguanosine kinase (dGK), deoxycytidine kinase (dCK), high Km 5'-nucleotidase (5'-NT), guanylate (GMP) kinase, nucleoside monophosphate (..
  42. ncbi HCN pacemaker channel activation is controlled by acidic lipids downstream of diacylglycerol kinase and phospholipase A2
    Keri J Fogle
    Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA
    J Neurosci 27:2802-14. 2007
    ..that 4betaPMA exerts its effects on HCN gating via sequential activation of PKC and diacylglycerol kinase (DGK) coupled with upregulation of MAPK (mitogen-activated protein kinase) and phospholipase A2 (PLA2), but its action ..
  43. ncbi Nitric oxide is critical for inducing phosphatidic acid accumulation in xylanase-elicited tomato cells
    Ana M Laxalt
    Instituto de Investigaciones Biologicas, Universidad Nacional de Mar del Plata, CC 1245, 7600 Mar del Plata, Argentina
    J Biol Chem 282:21160-8. 2007
    ..second messenger phosphatidic acid (PA) via the activation of the phospholipase C (PLC) and diacylglycerol kinase (DGK) pathway. Defense-related responses downstream of PA were studied...
  44. pmc Does aluminum generate a bonafide phospholipd signal cascade?
    Ana Ramos-Díaz
    Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán Mérida Yucatán, Mexico
    Plant Signal Behav 2:263-4. 2007
    ..The most important PA formation routes in plant signalling are: phospholipase C (PLC)/diacylglycerol kinase (DGK) and phospholipase D (PLD)...
  45. pmc Phosphatidic acid plays a regulatory role in clathrin-mediated endocytosis
    Costin N Antonescu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA
    Mol Biol Cell 21:2944-52. 2010
    ..by manipulating the activities and/or levels of either phospholipase D (PLD1 and PLD2) or diacylglycerol kinase (DGK), two enzyme classes involved in PA production...
  46. pmc Phosphatidic acid signaling regulation of Ras superfamily of small guanosine triphosphatases
    Yueqiang Zhang
    Department of Integrative Biology and Pharmacology University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    Biochim Biophys Acta 1791:850-5. 2009
    ..Recent studies indicate that the signaling PA generated from phospholipase D (PLD) and diacylglycerol kinase (DGK) plays critical roles in regulating the activity of some members of Ras superfamily of small guanosine ..
  47. ncbi Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells
    M Kai
    Department of Biochemistry, Sapporo Medical University School of Medicine, Japan
    J Biol Chem 269:18492-8. 1994
    In order to clone novel diacylglycerol kinase (DGK) isozymes, we first obtained a DGK-related cDNA fragment by polymerase chain reaction using the human hepatoma cell line HepG2 mRNA and degenerated primers...
  48. ncbi Activation of diacylglycerol kinase alpha is required for VEGF-induced angiogenic signaling in vitro
    Gianluca Baldanzi
    Department of Medical Sciences, University Amedeo Avogadro of Piemonte Orientale, v Solaroli 17, 28100, Novara, Italy
    Oncogene 23:4828-38. 2004
    ..As we had previously shown that Src-mediated activation of diacylglycerol kinase-alpha (Dgk-alpha) is required for hepatocytes growth factor-stimulated cell migration, we asked whether Dgk-alpha is involved ..
  49. pmc Molecular cloning of a Drosophila diacylglycerol kinase gene that is expressed in the nervous system and muscle
    I Masai
    Department of Physics, Faculty of Science, University of Tokyo, Japan
    Proc Natl Acad Sci U S A 89:6030-4. 1992
    We have isolated a Drosophila melanogaster diacylglycerol kinase (DGK, EC 2.7.1.107) homologue by using a porcine DGK cDNA probe and we have characterized its structure and expression...
  50. pmc Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans
    P Chen
    Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, 35294, USA
    J Bacteriol 180:167-70. 1998
    ..in mutacin II production, revealed that the transposon was inserted into the 3' region of a diacylglycerol kinase (dgk) gene. The insertion occurred in the same region as described for another S...
  51. ncbi Diacylglycerol kinases in signal transduction
    W J van Blitterswijk
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Chem Phys Lipids 98:95-108. 1999
    Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol (DAG) to phosphatidic acid. A family of nine mammalian isotypes have been identified...
  52. ncbi Diacylglycerol kinase in the central nervous system--molecular heterogeneity and gene expression
    K Goto
    Department of Anatomy, Yamagata University School of Medicine, Japan
    Chem Phys Lipids 98:109-17. 1999
    ..DAG kinase (DGK) phosphorylates DAG to generate phosphatidic acid, thus DGK is considered to be a regulator of PKC activity through ..
  53. pmc Role of diacylglycerol kinase alpha in the attenuation of receptor signaling
    M A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    J Cell Biol 153:207-20. 2001
    Diacylglycerol kinase (DGK) is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid...
  54. pmc Diacylglycerol kinase epsilon regulates seizure susceptibility and long-term potentiation through arachidonoyl- inositol lipid signaling
    E B Rodriguez de Turco
    Neuroscience Center of Excellence and Department of Ophthalmology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Proc Natl Acad Sci U S A 98:4740-5. 2001
    ..20:4-DAG signaling is terminated by its phosphorylation to phosphatidic acid, catalyzed by diacylglycerol kinase (DGK). We have cloned the murine DGKepsilon gene that showed, when expressed in COS-7 cells, selectivity for 20:4-DAG...
  55. pmc Diacylglycerol kinase delta suppresses ER-to-Golgi traffic via its SAM and PH domains
    Hisao Nagaya
    Department of Biochemistry, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Mol Biol Cell 13:302-16. 2002
    ..mutants of DGKdelta were also effective as the wild-type enzyme, suggesting that the catalytic activity of DGK was not involved in the present observation...
  56. ncbi Real-time quantitative PCR assays for deoxycytidine kinase, deoxyguanosine kinase and 5'-nucleotidase mRNA measurement in cell lines and in patients with leukemia
    E Mansson
    Department of Medicine, Division of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden
    Leukemia 16:386-92. 2002
    The relative levels of the deoxycytidine kinase (dCK), deoxyguanosine kinase (dGK), and the 5'-nucleotidase (5'-NT) are of importance for the effect of many nucleoside analogues used in the treatment of hematological malignancies...
  57. ncbi Low level of mitochondrial deoxyguanosine kinase is the dominant factor in acquired resistance to 9-beta-D-arabinofuranosylguanine cytotoxicity
    Kourosh Lotfi
    Department of Medicine and Care, Division of Clinical Pharmacology, Faculty of Health Sciences, Linkoping, SE 581 85, Sweden
    Biochem Biophys Res Commun 293:1489-96. 2002
    ..The mitochondrial enzyme deoxyguanosine kinase (dGK) and the nuclear/cytosol enzyme deoxycytidine kinase (dCK) are key enzymes in the activation of Ara-G...
  58. ncbi Alternative splicing of the human diacylglycerol kinase delta gene generates two isoforms differing in their expression patterns and in regulatory functions
    Fumio Sakane
    Department of Biochemistry, School of Medicine, Sapporo Medical University, South 1, West 17, Chuo Ku, Sapporo 060 8556, Japan
    J Biol Chem 277:43519-26. 2002
    Diacylglycerol kinase (DGK) plays an important role in signal transduction through modulating the balance between two signaling lipids, diacylglycerol and phosphatidic acid...
  59. ncbi Identification and characterization of two splice variants of human diacylglycerol kinase eta
    Tomohiro Murakami
    Department of Biochemistry, School of Medicine, Sapporo Medical University, South 1, West 17, Chuo Ku, Sapporo 060 8556, Japan
    J Biol Chem 278:34364-72. 2003
    Diacylglycerol kinase (DGK) participates in regulating the intracellular concentrations of two bioactive lipids, diacylglycerol and phosphatidic acid...
  60. ncbi Protein kinase C alpha phosphorylates and negatively regulates diacylglycerol kinase zeta
    Bai Luo
    Huntsman Cancer Institute, and Department of Oncological Sciences, University of Utah, Salt Lake City, Utah 84112, USA
    J Biol Chem 278:39542-7. 2003
    Diacylglycerol kinase (DGK) terminates diacylglycerol (DAG) signaling by phosphorylating DAG to produce phosphatidic acid, which also has signaling properties...
  61. ncbi Molecular insight into mitochondrial DNA depletion syndrome in two patients with novel mutations in the deoxyguanosine kinase and thymidine kinase 2 genes
    Liya Wang
    Department of Molecular Bioscience, Section of Veterinary Medical Biochemistry, SLU, The Biomedical Centre, P O Box 575, SE 751 23 Uppsala, Sweden
    Mol Genet Metab 84:75-82. 2005
    Thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK) are the two key enzymes in mitochondrial DNA (mtDNA) precursor synthesis...
  62. ncbi Expression and localization of diacylglycerol kinase isozymes and enzymatic features in rat lung
    Yuji Katagiri
    Dept of Anatomy and Cell Biology, Yamagata Univ School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Am J Physiol Lung Cell Mol Physiol 288:L1171-8. 2005
    Diacylglycerol kinase (DGK) catalyzes phosphorylation of diacylglycerol to generate phosphatidic acid, and both molecules are known to serve as second messengers as well as important intermediates for the synthesis of various lipids...
  63. ncbi Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant
    Plamen Georgiev
    The Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge, CB2 4AT, UK
    J Cell Sci 118:1373-84. 2005
    ..In Drosophila photoreceptors, lack of diacylglycerol kinase (DGK) activity (encoded by rdgA) leads to both constitutive TRP-channel activity and retinal degeneration...
  64. ncbi How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli
    Mark Lorch
    Centre for Biomolecular Magnetic Resonance and Institut für Biophysikalische Chemie, J W Goethe Universitat, Marie Curie Strasse 9, 60439 Frankfurt, Germany
    Chembiochem 6:1693-700. 2005
    ..we have prepared proteoliposomes and small crystals of the alpha-helical membrane-protein diacylglycerol kinase (DGK). Preparations were characterised by 13C- and 15N-cross-polarization magic-angle spinning (CPMAS) NMR...
  65. pmc Diacylglycerol kinase zeta regulates microbial recognition and host resistance to Toxoplasma gondii
    Cheng hu Liu
    Department of Pediatrics Allergy and Immunology, Duke University Medical Center, Durham, NC 27710, USA
    J Exp Med 204:781-92. 2007
    ..We demonstrate that the zeta isoform of the DGK family (DGKzeta) is expressed in macrophages (Mphi) and dendritic cells...
  66. ncbi Diacylglycerol kinases: why so many of them?
    Fumio Sakane
    Department of Biochemistry, Sapporo Medical University School of Medicine, South 1, West 17, Chuo Ku, Sapporo 060 8556, Japan
    Biochim Biophys Acta 1771:793-806. 2007
    Diacylglycerol (DAG) kinase (DGK) modulates the balance between the two signaling lipids, DAG and phosphatidic acid (PA), by phosphorylating DAG to yield PA. To date, ten mammalian DGK isozymes have been identified...
  67. pmc Diacylglycerol kinases in immune cell function and self-tolerance
    Xiao Ping Zhong
    Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
    Immunol Rev 224:249-64. 2008
    ..In T cells, two DGK isoforms, alpha and zeta, inhibit DAG-mediated signaling following T-cell receptor engagement and prevent T-cell ..
  68. pmc Mammalian diacylglycerol kinases: molecular interactions and biological functions of selected isoforms
    Matthew K Topham
    Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112, USA
    Biochim Biophys Acta 1790:416-24. 2009
    The mammalian diacylglycerol kinases (DGK) are a group of enzymes having important roles in regulating many biological processes. Both the product and the substrate of these enzymes, i.e...
  69. doi Brain trauma induces expression of diacylglycerol kinase zeta in microglia
    Tomoyuki Nakano
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990 9585, Japan
    Neurosci Lett 461:110-5. 2009
    Diacylglycerol kinase (DGK) is an enzyme which phosphorylates a second messenger diacylglycerol and consists of a family of isozymes that differ in terms of structural motifs, enzymological property, and cell and tissue distribution...
  70. doi Cardiological evaluation after cerebral ischaemia : Consensus statement of the Working Group Heart and Brain of the German Cardiac Society-Cardiovascular Research (DGK) and the German Stroke Society (DSG)
    Ulrich Laufs
    Klinik fur Innere Medizin III, Kardiologie, Angiologie und Internistische Intensivmedizin, Universitatsklinikum des Saarlandes, Homburg, Germany
    Clin Res Cardiol 99:609-25. 2010
    ....
  71. doi Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR
    Sandra J Ullrich
    Institute for Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
    Nat Chem Biol 7:263-70. 2011
    ..One such enzyme, the Escherichia coli integral membrane protein diacylglycerol kinase (DGK), is a key player in lipid regulation...
  72. pmc Differential regulation of diacylglycerol kinase isoform in human failing hearts
    Olga Bilim
    Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan
    J Cardiothorac Surg 6:65. 2011
    ..DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG...
  73. doi Non-invasive diagnosis of pulmonary hypertension: ESC/ERS Guidelines with Updated Commentary of the Cologne Consensus Conference 2011
    Ekkehard Grunig
    Center for Pulmonary Hypertension, Chest Clinic at University Hospital Heidelberg, Heidelberg, Germany
    Int J Cardiol 154:S3-12. 2011
    ..In June 2010, a Consensus Conference organized by the PH working groups of the German Society of Cardiology (DGK), the German Society of Respiratory Medicine (DGP) and the German Society of Pediatric Cardiology (DGPK) was held ..
  74. pmc Reactive oxygen species stimulate insulin secretion in rat pancreatic islets: studies using mono-oleoyl-glycerol
    Marylana Saadeh
    Department of Medicine, Obesity Research Center, School of Medicine, Boston University, Boston, Massachusetts, United States of America
    PLoS ONE 7:e30200. 2012
    ..Inhibition of diacylglycerol kinase (DGK) did not mimic the effect of MOG on insulin secretion, indicating that MOG did not act primarily by inhibiting DGK...
  75. doi DGKζ is degraded through the cytoplasmic ubiquitin-proteasome system under excitotoxic conditions, which causes neuronal apoptosis because of aberrant cell cycle reentry
    Masashi Okada
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990 9585, Japan
    Cell Signal 24:1573-82. 2012
    Recent reports have described the involvement of the diacylglycerol kinase (DGK) family in various pathological conditions...
  76. doi Diacylglycerol kinase zeta negatively regulates CXCR4-stimulated T lymphocyte firm arrest to ICAM-1 under shear flow
    Dooyoung Lee
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Integr Biol (Camb) 4:606-14. 2012
    ..However, how the level of DGK regulates chemokine-stimulated LFA-1-mediated T cell arrest under flow is unknown...
  77. doi Calcium negatively regulates an intramolecular interaction between the N-terminal recoverin homology and EF-hand motif domains and the C-terminal C1 and catalytic domains of diacylglycerol kinase α
    Masato Takahashi
    Department of Chemistry, Graduate School of Science, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Biochem Biophys Res Commun 423:571-6. 2012
    The type I diacylglycerol kinase (DGK) isozymes (α, β and γ) contain a shared recoverin homology (RVH) domain, a tandem repeat of Ca2+-binding EF-hand motifs, two cysteine-rich C1 domains, and the catalytic domain...
  78. doi Novel leukemic cell lines resistant to clofarabine by mechanisms of decreased active metabolite and increased antiapoptosis
    Hiroko Shigemi
    Division of Hematology and Oncology, University of Fukui, Eiheiji, Japan
    Cancer Sci 104:732-9. 2013
    ..to the active metabolite CAFdA triphosphate (CAFdATP) by deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Two novel CAFdA-resistant variants were established and their mechanism of resistance was elucidated...
  79. pmc cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1
    Kai Cai
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    J Lipid Res 54:2121-32. 2013
    Diacylglycerol kinase (DGK)θ is a lipid kinase that phosphorylates diacylglycerol to form phosphatidic acid (PA)...
  80. pmc The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos
    Shuji Ueda
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan
    BMC Dev Biol 13:35. 2013
    Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and is involved in a variety of physiological functions. The isoform DGKθ has a unique domain structure and is the sole member of type V DGK...
  81. pmc Alternative splicing generates a diacylglycerol kinase α transcript that acts as a dominant-negative modulator of superoxide production in localized aggressive periodontitis
    Eraldo L Batista
    Department of Diagnostics and Surgical Sciences and Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, MB, Canada previously, Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA
    J Periodontol 85:934-43. 2014
    ..Members of the DGK family undergo alternative splicing, generating the protein diversity necessary to control different intracellular ..
  82. pmc Phenotypic expansion of DGKE-associated diseases
    Rik Westland
    Division of Nephrology, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York Department of Pediatric Nephrology, VU University Medical Center, Amsterdam, The Netherlands
    J Am Soc Nephrol 25:1408-14. 2014
    ..The recent discovery of loss-of-function mutations in DGKE in patients with aHUS and normal complement levels challenged this observation...
  83. doi [Pathophysiology of atypical hemolytic uremic syndrome. Ten years of progress, from laboratory to patient]
    Veronique Fremeaux-Bacchi
    INSERM UMR_S1135, Centre de Recherche des Cordeliers, Équipe Complément et Maladies, 15 rue de l Ecole de Medecine, 75006 Paris, France Hôpital européen Georges Pompidou, Laboratoire d Immunologie, 20 rue Leblanc, 75015 Paris, France
    Biol Aujourdhui 207:231-40. 2013
    ..Mutations in the gene of thrombomodulin and diacylglycerol kinase epsilon (DGKe) have been reported, suggesting the possibility of an alternative or more complex disease-..
  84. doi Cellular expression and localization of DGKζ-interacting NAP1-like proteins in the brain and functional implications under hypoxic stress
    Nobuya Takahashi
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, 2 2 2 Iida nishi, Yamagata, 990 9585, Japan
    Histochem Cell Biol 142:461-71. 2014
    Diacylglycerol kinase (DGK) catalyzes conversion of a lipid second messenger diacylglycerol to another messenger molecule phosphatidic acid...
  85. doi Mitochondrial thymidine kinase 2 but not deoxyguanosine kinase is up-regulated during the stationary growth phase of cultured cells
    Ren Sun
    a Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, BMC, Uppsala, Sweden
    Nucleosides Nucleotides Nucleic Acids 33:282-6. 2014
    Mitochondrial thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK) catalyze the initial phosphorylation of pyrimidine and purine deoxyribonucleosides, and are essential for maintaining mitochondrial dNTP pools for mitochondrial DNA ..
  86. doi Role of the N-terminal hydrophobic residues of DGKε in targeting the endoplasmic reticulum
    Hirooki Matsui
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata 990 9585, Japan Department of Otolaryngology, Head and Neck Surgery, Yamagata University School of Medicine, Yamagata 990 9585, Japan
    Biochim Biophys Acta 1842:1440-50. 2014
    ..The diacylglycerol kinase (DGK) enzyme family catalyzes phosphorylation of diacylglycerol to phosphatidic acid...
  87. pmc Diacylglycerol kinase α establishes T cell polarity by shaping diacylglycerol accumulation at the immunological synapse
    Anne Chauveau
    Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Sci Signal 7:ra82. 2014
    ..We used a single-cell photoactivation approach to demonstrate that diacylglycerol kinase α (DGK-α), which catalyzes the conversion of DAG to phosphatidic acid, determined T cell polarity by limiting the ..
  88. pmc Diacylglycerol kinase α promotes 3D cancer cell growth and limits drug sensitivity through functional interaction with Src
    Pedro Torres-Ayuso
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CSIC, Madrid, Spain
    Oncotarget 5:9710-26. 2014
    Diacylglycerol kinase (DGK)α converts diacylglycerol to phosphatidic acid...
  89. doi [Commentary by the German Society for Thoracic and Cardiovascular Surgery on the positions statement by the German Cardiology Society on quality criteria for transcatheter aortic valve implantation (TAVI)]
    Jochen Cremer
    Klinik für Herz und Gefäßchirurgie, UK Schleswig Holstein, Campus Kiel, Kiel, Germany
    Thorac Cardiovasc Surg 62:639-44. 2014
    ..To be noted, most of these hospitals perform less than 20 cases per year. Recently, the German Cardiac Society (DGK) published a position paper supporting this practice pattern...
  90. doi The effects of valproate and olanzapine on the abnormal behavior of diacylglycerol kinase β knockout mice
    Mitsue Ishisaka
    Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan
    Pharmacol Rep 67:275-80. 2015
    Diacylglycerol kinase (DGK) is an enzyme that converts diacylglycerol to phosphatidic acid...
  91. pmc TCR signaling intensity controls CD8+ T cell responsiveness to TGF-β
    Vidhyalakshmi Arumugam
    Blood Research Institute, Department of Microbiology and Molecular Genetics, and Division of Hematology and Oncology, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA and Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA
    J Leukoc Biol 98:703-12. 2015
    b>DGK-ζ is a negative regulator of TCR signaling that causes degradation of the second messenger DAG, terminating DAG-mediated activation of Ras and PKCθ...
  92. pmc Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production
    Jili Zhu
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas Department of Nephrology, Renmin Hospital, Wuhan University, Hubei, Wuhan, China
    Am J Physiol Renal Physiol 310:F895-908. 2016
    ..we and others have described a genetic form of TMA caused by mutations in the gene diacylglycerol kinase-ε (DGKE) that encodes the lipid kinase DGKε (Lemaire M, Fremeaux-Bacchi V, Schaefer F, Choi MR, Tang WH, Le Quintrec M, ..
  93. doi DGKζ Downregulation Enhances Osteoclast Differentiation and Bone Resorption Activity Under Inflammatory Conditions
    Kiyoshi Iwazaki
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Yamagata, Japan
    J Cell Physiol 232:617-624. 2017
    ..In this study, we examined the functional implications of DG kinase (DGK), an enzyme family responsible for DG metabolism, for osteoclast differentiation and activity...
  94. doi Distinct 1-monoacylglycerol and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes
    Yuriko Sato
    Department of Chemistry, Graduate School of Science, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Biochim Biophys Acta 1864:1170-6. 2016
    Diacylglycerol kinase (DGK) consists of ten isozymes and is involved in a wide variety of patho-physiological events. However, the enzymological properties of DGKs have not been fully understood...
  95. doi Diacylglycerol kinases in cancer
    Isabel Merida
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia CNB CSIC, E 28049, Madrid, Spain Electronic address
    Adv Biol Regul . 2016
    Diacylglycerol kinases (DGK) are a family of enzymes that catalyze the transformation of diacylglycerol into phosphatidic acid...
  96. pmc Arachidonoyl-Specific Diacylglycerol Kinase ε and the Endoplasmic Reticulum
    Tomoyuki Nakano
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine Yamagata, Japan
    Front Cell Dev Biol 4:132. 2016
    ..One enzyme responsible for PA synthesis is diacylglycerol kinase (DGK) that phosphorylates diacylglycerol (DG) to PA...
  97. ncbi Neutral lipid accumulation in the membranes of Escherichia coli mutants lacking diglyceride kinase
    C R Raetz
    J Biol Chem 253:3882-7. 1978
    ..The gene (designated dgk) which is altered in these mutants is cotransduceable with the malB locus, near minute 90 on the chromosome...
  98. ncbi The DNA sequences encoding plsB and dgk loci of Escherichia coli
    V A Lightner
    J Biol Chem 258:10856-61. 1983
    ..from Escherichia coli containing plsB, the structural gene for the sn-glycerol-3-phosphate acyltransferase, and the dgk locus, believed to encode diglyceride kinase...
  99. ncbi Mutations within the putative active site of heterodimeric deoxyguanosine kinase block the allosteric activation of the deoxyadenosine kinase subunit
    Inshik Park
    Faculty of Food Science, Dong A University, Pusan 604 714, Korea
    J Biochem Mol Biol 35:244-7. 2002
    ..Asp-84 residue of the deoxyguanosine kinase subunit of the tandem deoxyadenosine kinase/ deoxyguanosine kinase (dAK/dGK) from Lactobacillus acidophilus R-26 by Ala, Asn, or Glu produced increased Km values for deoxyguanosine on dGK...
  100. ncbi Substrate preference of stress-activated phospholipase D in Chlamydomonas and its contribution to PA formation
    Steven A Arisz
    Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, NL 1098 SM, Amsterdam, The Netherlands
    Plant J 34:595-604. 2003
    ..pathways via phospholipase D (PLD) and via phospholipase C (PLC) in combination with diacylglycerol kinase (DGK)...
  101. ncbi Diacylglycerol kinase zeta is involved in the process of cerebral infarction
    Tomoyuki Nakano
    Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida nishi 2 2 2, Yamagata 990 9585, Japan
    Eur J Neurosci 23:1427-35. 2006
    Diacylglycerol kinase (DGK) is an enzyme that phosphorylates a second messenger diacylglycerol (DG) and is involved in a variety of pathophysiological cellular responses...

Research Grants29

  1. XIAOPING ZHONG; Fiscal Year: 2016
    ..In aim 2, we will investigate mechanisms by which DGK activity contributes to T cell anergy...
  2. Matthew J Riese; Fiscal Year: 2014
    ..We have found a new target for treating cancer, dgk-zeta, which awakens the immune system in mice, preventing cancer growth...
  3. Daniel M Raben; Fiscal Year: 2016
    ..in this revised proposal focus on understanding the regulation and physiological role of a diacylglycerol kinase (DGK), DGK-theta...
  4. DGK in T Cell Regulation and Tolerance
    Thomas F Gajewski; Fiscal Year: 2010
    ..I cells have been characterized to have a defect in TCR/CD28- L]' (Ti 'D- of the diacylglycerol kinase (DGK) family, in particular DGK-u, participate in the suppression of targeted genes in the post-thymic I cell ..
  5. CRYSTALLIZATION OF DIACYLGLYCEROL KINASE
    JAMES BOWIE; Fiscal Year: 2000
    ..crystallize and solve the structure of an integral membrane protein, diacylglycerol kinase from Escherichia coli (DGK). DGK catalyzes the conversion of diacylglycerol and ATP to phosphatidic acid and ADP...
  6. Thomas F Gajewski; Fiscal Year: 2016
    ..We previously have shown that upregulated expression of lipid kinases of the diacylglycerol kinase (DGK) family participate in the suppression of RasGRP-mediated Ras activation and contribute to T cell dysfunction in ..
  7. CLONING / CHARACTERIZATION OF NOVEL DIACYLGLYCEROL KINAS
    Susan Jaken; Fiscal Year: 1999
    Diacylglycerol kinase (DGK) catalyzes the conversion of diacylglycerol (DG) to phosphatidic acid. DG is a key second messenger that regulates the activity of protein kinase C (PKC)...
  8. ENZYMOLOGY OF GOLGI STACK FORMATION
    Vivek Malhotra; Fiscal Year: 2007
    ..DAG generated as a result is metabolized, post-fission, by PKD dependent activation of diacylglycerol kinase(DGK)0 (aim#2)...
  9. Molecular Dissection of T Cell Anergy
    Thomas Gajewski; Fiscal Year: 2005
    ..One of these is diacylglycerol kinase (DGK)...
  10. John K Buolamwini; Fiscal Year: 2015
    ..rate-limiting monophosphorylation by mitochondrial-specific thymidine kinase 2 (TK-2) and/or deoxyguanosine kinase (dGK). Mitochondrial membrane nucleoside transporters (NTs) import NRTIs from the cytoplasm...
  11. Roberta Faccio; Fiscal Year: 2015
    ..In order to study the role of DAG, we now turned our attention to DGK?, an enzyme that decreases cellular levels of DAG by converting it to phosphatidic acid (PA)...
  12. A Murine Model of Chronic Autoimmune Hepatitis and Liver Fibrosis
    XIAOPING ZHONG; Fiscal Year: 2012
    ..We and others have recently demonstrated that DGK[unreadable] and [unreadable], isoforms expressed in T cells, negatively control T cell activation by inhibiting T ..
  13. MORGAN A HUSE; Fiscal Year: 2016
    ..that are required for stable DAG accumulation and reorientation of the MTOC;2) Determine the roles played by DGK-1 and DGK-6 in shaping the polarizing DAG gradient;and 3) Determine the importance of Marcksl1 dynamics during MTOC ..
  14. Daniel A Hammer; Fiscal Year: 2016
    ..cells and T cells from knock-out mice in which SLP-76 is deleted or altered, or in which diacylglycerol kinases (DGK) have been deleted...
  15. Immunotherapy for Peanut Allergy
    XIAOPING ZHONG; Fiscal Year: 2009
    ..Furthermore, our preliminary data revealed that the cell signaling molecule diacylglycerol (DAG) kinase? (DGK?), which converts DAG to phosphatidic acid (PA) through phosphorylation, positively regulates TLR-induced IL-12 ..
  16. Prevention and Treatment of Cashew Allergy via Pepsinized Allergens
    MICHAEL DAVID KULIS; Fiscal Year: 2010
    ..sensitizing protocol in the C3H/HeJ-cholera toxin model [2] as well as our novel model of food allergy in DGK-zeta knockout mice...
  17. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
    ..aims are to: 1) test the hypothesis that deficiency in PAF-receptor and/or in arachidonyl-diglyceride-selective DGK-e result in decreased susceptibility to epileptogenesis; 2) identify upstream signaling modified as a consequence ..
  18. Coordinate Regulation Of Nuclear DGK-theta and PLD
    DANIEL RABEN; Fiscal Year: 2009
    ..We believe that an understanding of nuclear lipid signaling is key to our ability to intervene in events such as wound repair and tissue remodeling. ..
  19. NOVEL SUBSTRATES OF THE TCR KINASE
    Gary Koretzky; Fiscal Year: 2005
    ..We anticipate that collectively these studies will provide new insights into how this protein (and by extension other proteins with similar functional domains) may function in the regulation of cellular activation. ..
  20. Regulation of T Cell and Mast Cell Function by DGKzeta
    Gary A Koretzky; Fiscal Year: 2010
    ..We anticipate that this work will shed additional light on the complex regulation of T cell and mast cell function and will hopefully suggest novel approaches to modulate activity of these cell types. ..
  21. SIGNAL TRANSDUCTION IN THE IMMUNE SYSTEM-FASEB CONF.
    Gary Koretzky; Fiscal Year: 2003
    ..3) To continue a biannual forum for investigators interested in a comprehensive focus on signaling events in the immune system. ..
  22. THE ROLE OF SLP-76 IN PLATELET SIGNAL TRANSDUCTION
    Gary Koretzky; Fiscal Year: 2003
    ..It is anticipated that these studies collectively will provide new insights into the biology of SLP-76 as a regulator of signaling events in platelets. ..
  23. Regulation of PMN activation by SLP-76, PRAM-1 and ADAP
    Gary Koretzky; Fiscal Year: 2008
    ....
  24. NEUROBIOLOGY OF DYSTONIA
    HYDER JINNAH; Fiscal Year: 2008
    ..In addition, the studies will have direct relevance for modern neurosurgical approaches to the management of dystonia that involve focal brain stimulation or lesion techniques. [unreadable] [unreadable]..
  25. Lymphocyte Activation and Signaling
    Gary Koretzky; Fiscal Year: 2006
    ..abstract_text> ..
  26. 2005 Immunochemistry and Immunobiology Gordon Conference
    Gary Koretzky; Fiscal Year: 2005
    ..These talks will be selected from among the submitted abstracts. ..
  27. SIGNAL TRANSDUCTION IN THE IMMUNE SYSTEM-FASEB CONF.
    Gary Koretzky; Fiscal Year: 2005
    ..3) To continue a biannual forum for investigators interested in a comprehensive focus on signaling events in the immune system. ..
  28. STRUCTURE/FUNCTION OF SH2 DOMAIN OF SLP 76
    Gary Koretzky; Fiscal Year: 2002
    ..Collectively, we hope these studies will provide insights into the complex regulation of signaling by SLP-76 and LAT in T cell development and activation. ..
  29. Neurobiology of Self-Injurious Behavior
    HYDER JINNAH; Fiscal Year: 2005
    ..These studies have direct relevance for understanding the neuroanatomical and neurochemical basis for self-injurious behavior and direct implications for a potential novel treatment strategy. ..