DGAT1

Summary

Gene Symbol: DGAT1
Description: diacylglycerol O-acyltransferase 1
Alias: ARAT, ARGP1, DGAT, DIAR7, diacylglycerol O-acyltransferase 1, ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, acyl-CoA retinol O-fatty-acyltransferase, acyl-CoA:diacylglycerol acyltransferase, diacylglycerol O-acyltransferase homolog, diglyceride acyltransferase
Species: human

Top Publications

  1. ncbi CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine
    Fatiha Nassir
    Department of Medicine, Division of Nutritional Science, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:19493-501. 2007
  2. pmc Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor
    Ana Arabolaza
    Microbiology Division, Instituto de Biologia Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha, Rosario, Argentina
    Appl Environ Microbiol 74:2573-82. 2008
  3. pmc Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1
    Eva Herker
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, USA
    Nat Med 16:1295-8. 2010
  4. pmc Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption
    Dong Cheng
    Department of Metabolic Diseases, Bristol Myers Squibb Company, Princeton, New Jersey 08543 5400, USA
    J Biol Chem 283:29802-11. 2008
  5. ncbi Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts
    Carolina Bagnato
    Instituto de Investigaciones Bioquímicas de La Plata INIBIOLP, CONICET UNLP, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, calles 60 y 120, 1900 La Plata, Argentina
    J Biol Chem 278:52203-11. 2003
  6. ncbi Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat
    S J Smith
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California, USA
    Nat Genet 25:87-90. 2000
  7. pmc Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
    Chi Liang Eric Yen
    Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA
    J Lipid Res 49:2283-301. 2008
  8. ncbi Re-evaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease
    Motoyuki Kohjima
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 812 8582 Fukuoka, Japan
    Int J Mol Med 20:351-8. 2007
  9. doi Effects of the peroxisome proliferator-activated receptor-alpha agonists clofibrate and fish oil on hepatic fatty acid metabolism in weaned dairy calves
    N B Litherland
    Department of Animal Sciences, University of Illinois, Urbana 61801, USA
    J Dairy Sci 93:2404-18. 2010
  10. doi Cloning and molecular characterization of the acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) gene from Echium
    A Mañas-Fernández
    Laboratorio Genética III, Edificio CITE II B, Universidad de Almeria, 04120, Almeria, Spain
    Lipids 44:555-68. 2009

Research Grants

  1. Role of DGAT1 in Mammary Gland Development and Cancer
    Sylvaine Cases; Fiscal Year: 2006
  2. IVAN PETER URAY; Fiscal Year: 2014
  3. Cardiac metabolism and heart failure
    Rong Tian; Fiscal Year: 2010
  4. Functional Analysis of MGAT Enzymes
    Robert V Farese; Fiscal Year: 2010
  5. Michael D Jensen; Fiscal Year: 2016
  6. SUNEIL KRISHNA KOLIWAD; Fiscal Year: 2014
  7. Robert V Farese; Fiscal Year: 2014
  8. DGAT1: Linking Fatty Acids to Inflammation and Metabolism in White Adipose Tissue
    SUNEIL KRISHNA KOLIWAD; Fiscal Year: 2013
  9. Alcohol Intake and Hepatic Fat Metabolism
    Li Shin Huang; Fiscal Year: 2012
  10. Human B-cell metabolic signaling under chronic nutrient overload
    Guim Kwon; Fiscal Year: 2012

Detail Information

Publications233 found, 100 shown here

  1. ncbi CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine
    Fatiha Nassir
    Department of Medicine, Division of Nutritional Science, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:19493-501. 2007
    ..In Cd36(-/-) S1, mRNA for L-fabp, Dgat1, and apoA-IV was reduced...
  2. pmc Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor
    Ana Arabolaza
    Microbiology Division, Instituto de Biologia Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Suipacha, Rosario, Argentina
    Appl Environ Microbiol 74:2573-82. 2008
    ..significantly resemble the recently identified wax ester synthase/acyl-coenzyme A (CoA):DAG acyltransferase (DGAT) from Acinetobacter baylyi...
  3. pmc Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1
    Eva Herker
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, USA
    Nat Med 16:1295-8. 2010
    ..Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection...
  4. pmc Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption
    Dong Cheng
    Department of Metabolic Diseases, Bristol Myers Squibb Company, Princeton, New Jersey 08543 5400, USA
    J Biol Chem 283:29802-11. 2008
    Acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) is one of the four intestinal membrane bound acyltransferases implicated in dietary fat absorption...
  5. ncbi Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts
    Carolina Bagnato
    Instituto de Investigaciones Bioquímicas de La Plata INIBIOLP, CONICET UNLP, Facultad de Ciencias Medicas, Universidad Nacional de La Plata, calles 60 y 120, 1900 La Plata, Argentina
    J Biol Chem 278:52203-11. 2003
    ..To test this hypothesis, we stably overexpressed the DGAT1 mouse gene in human lung SV40-transformed fibroblasts (DGAT cells), which contains high levels of DAG...
  6. ncbi Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat
    S J Smith
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California, USA
    Nat Genet 25:87-90. 2000
    ..Triglyceride synthesis has been assumed to occur primarily through acyl CoA:diacylglycerol transferase (Dgat), a microsomal enzyme that catalyses the final and only committed step in the glycerol phosphate pathway...
  7. pmc Thematic review series: glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
    Chi Liang Eric Yen
    Department of Nutritional Sciences, University of Wisconsin, Madison, WI 53706, USA
    J Lipid Res 49:2283-301. 2008
    ..The genes encoding two DGAT enzymes, DGAT1 and DGAT2, were identified in the past decade, and the use of molecular tools, including mice deficient in either ..
  8. ncbi Re-evaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease
    Motoyuki Kohjima
    Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 812 8582 Fukuoka, Japan
    Int J Mol Med 20:351-8. 2007
    ..catalase, and glutathione synthetase (GSS) for antioxidant pathways; and diacylglycerol O-acyltransferase 1 (DGAT1), PPARgamma, and hormone-sensitive lipase (HSL) for triglyceride synthesis and catalysis...
  9. doi Effects of the peroxisome proliferator-activated receptor-alpha agonists clofibrate and fish oil on hepatic fatty acid metabolism in weaned dairy calves
    N B Litherland
    Department of Animal Sciences, University of Illinois, Urbana 61801, USA
    J Dairy Sci 93:2404-18. 2010
    ..whereas fish oil did not significantly affect genes associated with fatty acid oxidation but tended to increase DGAT1. Overall, our data indicated that bovine liver responded to clofibrate treatment but not fish oil, although ..
  10. doi Cloning and molecular characterization of the acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) gene from Echium
    A Mañas-Fernández
    Laboratorio Genética III, Edificio CITE II B, Universidad de Almeria, 04120, Almeria, Spain
    Lipids 44:555-68. 2009
    ..a gene structure composed of 16 exons, yielding a protein (EpDGAT) of 473 amino acids with high similarity to DGAT1 enzymes of plants...
  11. ncbi Effect of 3- and 4-thia-substituted fatty acids on glycerolipid metabolism and mitochondrial beta-oxidation in rat liver
    J Skorve
    Laboratory of Clinical Biochemistry, University of Bergen, Haukeland Sykehus, Norway
    Biochem Pharmacol 40:2005-12. 1990
    ..Administration of CETTD marginally affected the isoenzymes of GPAT. Diacylglycerol acyltransferase (DGAT) activity was increased by 35% in BCMTD and CMTTD treated rats, but by administration of CETTD the enzyme activity ..
  12. ncbi Concerted elevation of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin
    Rupalie L Meegalla
    Department of Central Nervous System Diseases, Bristol Myers Squibb Company, Experimental Station, E400 5269, Rt 141 and Henry Clay Road, Wilmington, DE 19880, USA
    Biochem Biophys Res Commun 298:317-23. 2002
    ..In the current study, we report that glucose preferentially enhances DGAT1 mRNA expression, whereas insulin specifically increases the level of DGAT2 mRNA...
  13. ncbi The effects of dietary n-3 polyunsaturated fatty acids delivered in chylomicron remnants on the transcription of genes regulating synthesis and secretion of very-low-density lipoprotein by the liver: modulation by cellular oxidative state
    Kathleen M Botham
    Department of Veterinary Basic Sciences, Royal Veterinary College, London NW1 0TU, United Kingdom
    Exp Biol Med (Maywood) 228:143-51. 2003
    ..microsomal triacylglycerol transfer protein (MTP), and the enzymes acyl coenzyme A:diacylglycerol acyltransferase (DGAT) and acyl coenzyme A:cholesterol acyltransferase 2 (ACAT2), which play a role in the regulation of triacylglycerol ..
  14. doi CCAAT/enhancer binding protein alpha (C/EBPalpha) in adipose tissue regulates genes in lipid and glucose metabolism and a genetic variation in C/EBPalpha is associated with serum levels of triglycerides
    Louise E Olofsson
    Department of Molecular and Clinical Medicine, Sahlgrenska University Hospital, Vita stråket 15, 413 45 Gothenburg, Sweden
    J Clin Endocrinol Metab 93:4880-6. 2008
    ..CCAAT/enhancer binding protein alpha (C/EBPalpha) is a transcription factor involved in adipogenesis and hepatic glucose and lipid metabolism...
  15. ncbi A mathematical model for mammary fatty acid synthesis and triglyceride assembly: the role of stearoyl CoA desaturase (SCD)
    Paul R Shorten
    Mathematical Biology Group, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton, New Zealand
    J Dairy Res 71:385-97. 2004
    ..Our model also predicted that a K232A mutation in the bovine mammary DGAT1 gene that is linked with an increase in milk fat yield would be consistent with a 120% increase in the DGAT ..
  16. ncbi Increased very low density lipoprotein secretion and gonadal fat mass in mice overexpressing liver DGAT1
    Tomomi Yamazaki
    Division of Clinical Nutrition, National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    J Biol Chem 280:21506-14. 2005
    ..The genes for two DGAT enzymes, DGAT1 and DGAT2, have been identified...
  17. doi Cloning and functional analysis of two type 1 diacylglycerol acyltransferases from Vernonia galamensis
    Keshun Yu
    Department of Plant and Soil Science, University of Kentucky, 1405 Veterans Drive, Lexington, KY 40546 0091, USA
    Phytochemistry 69:1119-27. 2008
    ..There are two classes of DGATs known as DGAT1 and DGAT2. Here we report on the isolation, characterization, and functional analysis of two DGAT1 cDNAs from V...
  18. ncbi Antisense oligonucleotide reduction of DGAT2 expression improves hepatic steatosis and hyperlipidemia in obese mice
    Xing Xian Yu
    Department of Antisense Drug Discovery, Isis Pharmaceuticals, Inc, 2292 Faraday Avenue, Carlsbad, CA 92008, USA
    Hepatology 42:362-71. 2005
    ..ASO treatment reduced DGAT2 messenger RNA (mRNA) levels by more than 75% in both liver and fat but did not change DGAT1 mRNA levels in either of these tissues, which resulted in decreased DGAT activity in liver but not in fat...
  19. pmc Expression profiling of hepatic genes associated with lipid metabolism in nephrotic rats
    Yunfeng Zhou
    Department of Physiology and Pathophysiology, Peking University Health Science Center, 38 Xueyuan Rd, Beijing, China
    Am J Physiol Renal Physiol 295:F662-71. 2008
    ..biosynthesis, Acc, FAS, ELOVL 2, and ELOVL6, and three critical for triglyceride biosynthesis, Gpam, Agpat 3, and Dgat 1, were significantly upregulated, whereas two genes involved in fatty acid oxidation, Dci and MCAD, were ..
  20. ncbi DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis
    Kimberly K Buhman
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Biol Chem 277:25474-9. 2002
    ..In this study, we analyzed intestinal triacylglycerol absorption and chylomicron synthesis and secretion in DGAT1-deficient (Dgat1(-/-)) mice...
  21. ncbi Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene
    Jing Yuan
    State Key Laboratory for Agrobiotechnology, China Agricultural University, No 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, P R China
    Biochem Genet 45:611-21. 2007
    We report molecular cloning and single nucleotide polymorphism detection of the buffalo DGAT1 gene. Diacylglycerol acyltransferase (DGAT1) is considered the key enzyme in controlling the rate of synthesis of triglycerides...
  22. doi Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content
    Jingyu Xu
    National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Canada
    Plant Biotechnol J 6:799-818. 2008
    A full-length cDNA encoding a putative diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) was obtained from Tropaeolum majus (garden nasturtium)...
  23. doi Comparative genomics and proteomics of vertebrate diacylglycerol acyltransferase (DGAT), acyl CoA wax alcohol acyltransferase (AWAT) and monoacylglycerol acyltransferase (MGAT)
    Roger S Holmes
    School of Biomolecular and Physical Sciences, Griffith University, Nathan 4111 Brisbane, Queensland, Australia
    Comp Biochem Physiol Part D Genomics Proteomics 5:45-54. 2010
    ..C. 2.3.1.22) and diacylglycerol acyltransferase-like (DGAT) (E.C. 2.3.1.20) genes and proteins, including DGAT1, DGAT2, DGAT2L6 (DGAT2-like protein 6), AWAT1 (acyl CoA wax alcohol acyltransferase 1), AWAT2, MGAT1, MGAT2 and ..
  24. ncbi Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase
    P Bouvier-Nave
    Institut de Biologie Moleculaire des Plantes, Strasbourg, France
    Eur J Biochem 267:85-96. 2000
    ..Moreover, during the preparation of microsomes from the Arabidopsis DGAT-transformed yeast, a floating layer was observed on top of the 100 000 g supernatant...
  25. ncbi Depot-specific modulation of rat intraabdominal adipose tissue lipid metabolism by pharmacological inhibition of 11beta-hydroxysteroid dehydrogenase type 1
    Magalie Berthiaume
    Laval Hospital Research Center, Department of Anatomy and Physiology, Laval University, Quebec, Canada G1V 4G5
    Endocrinology 148:2391-7. 2007
    ..fat, the inhibitor decreased (to 25-50% of control) mRNA levels of genes involved in lipid synthesis (FAS, SCD1, DGAT1) and fatty acid cycling (lipolysis/reesterification, ATGL and PEPCK) and increased (30%) the activity of the fatty ..
  26. pmc Transgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts
    Nirmala Sharma
    National Research Council Canada, Plant Biotechnology Institute, NRC, 101 Gymnasium Place, Saskatoon SKS7N0W9, Canada
    BMC Genomics 9:619. 2008
    ..Previous research identified microsomal diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) as controlling a rate-limiting step in the TAG biosynthesis pathway...
  27. pmc Beyond triglyceride synthesis: the dynamic functional roles of MGAT and DGAT enzymes in energy metabolism
    Yuguang Shi
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, 500 University Dr, Hershey, PA, USA
    Am J Physiol Endocrinol Metab 297:E10-8. 2009
    ..The metabolic complexity of TAG synthesis is reflected by the presence of multiple isoforms of MGAT and DGAT enzymes that differ in catalytic properties, subcellular localization, tissue distribution, and physiological ..
  28. doi Testing candidate gene effects on milk production traits in dairy cattle under various parameterizations and modes of inheritance
    T Suchocki
    Department of Animal Genetics, Wrocław University of Environmental and Life Sciences, Kozuchowska 7, 51 631 Wrocław, Poland
    J Dairy Sci 93:2703-17. 2010
    ..effects of the selected polymorphisms located within butyrophilin (BTN1A1), diacylglycerol acyltransferase 1 (DGAT1), leptin (LEP), and leptin receptor (LEPR) candidate genes on milk production traits using data from 2 dairy ..
  29. ncbi Sex differences in lipid metabolism during reproduction in free-living tree lizards (Urosaurus ornatus)
    Eva L Lacy
    Department of Biology, Georgia State University, Atlanta, GA 30303 3083, USA
    Gen Comp Endocrinol 128:180-92. 2002
    ..variation in abdominal fat body mass, liver total lipid, the activity of an enzyme regulating fat storage (DGAT) in both of these tissues, and circulating free fatty acids across the reproductive season...
  30. pmc Sequential regulation of diacylglycerol acyltransferase 2 expression by CAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPalpha during adipogenesis
    Victoria A Payne
    Department of Clinical Biochemistry, University of Cambridge, Box 232, Level 4, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2QR, United Kingdom
    J Biol Chem 282:21005-14. 2007
    ..Despite the existence of an alternative acyltransferase (DGAT1), mice lacking DGAT2 have a severe deficiency of TG in adipose tissue, indicating a nonredundant role for this ..
  31. doi Fish oil fed prior to ethanol administration prevents acute ethanol-induced fatty liver in mice
    Satoshi Wada
    Nutritional Science Program, National Institute of Health and Nutrition, 1 23 1, Toyama, Shinjuku ku, Tokyo 162 8636, Japan
    J Hepatol 49:441-50. 2008
    ..We examined whether dietary fish oil can prevent acute ethanol (alcohol)-induced fatty liver...
  32. doi The DGAT2 gene is a candidate for the dissociation between fatty liver and insulin resistance in humans
    Konstantinos Kantartzis
    Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Medicine, Nephrology and Clinical Chemistry, University of Tubingen, 72076 Tubingen, Germany
    Clin Sci (Lond) 116:531-7. 2009
    The enzyme DGAT (acyl-CoA:diacylglycerol acyltransferase) catalyses the final step of triacylglycerol (triglyceride) synthesis...
  33. doi DGAT1, DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants
    Runzhi Li
    Department of Plant and Soil Science, University of Kentucky, Lexington, KY 40546, USA
    Lipids 45:145-57. 2010
    Triacylglycerol (TAG) is the main storage lipid in plants. Acyl-CoA: diacylglycerol acyltransferase (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferase (PDAT) can catalyze TAG synthesis...
  34. ncbi The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene
    J Zou
    National Research Council of Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada
    Plant J 19:645-53. 1999
    ..line AS11, an EMS-induced mutation at a locus on chromosome II results in a reduced diacylglycerol acyltransferase (DGAT; EC 2.3.1...
  35. ncbi A citrus polymethoxy flavonoid, nobiletin inhibits sebum production and sebocyte proliferation, and augments sebum excretion in hamsters
    Takashi Sato
    Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
    J Invest Dermatol 127:2740-8. 2007
    ..TG) level and the size of sebaceous glands along with inhibition of diacylglycerol acyltransferase (DGAT)-dependent TG synthesis and sebocyte proliferation...
  36. ncbi Osthole improves fat milk-induced fatty liver in rats: modulation of hepatic PPAR-alpha/gamma-mediated lipogenic gene expression
    Yan Zhang
    Department of Pharmacology, Medical School of Soochow University, Suzhou, PR China
    Planta Med 73:718-24. 2007
    ..g., 53.4 - 93.2 % for CYP7A, 21.1 - 63.2 % for L-FABP and 34.1 - 57.3 % for FATP4, while the DGAT mRNA expression was decreased by 26.0 - 44.4 %...
  37. doi Bacterial acyltransferases as an alternative for lipase-catalyzed acylation for the production of oleochemicals and fuels
    Tim Stöveken
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, 48149 Munster, Germany
    Angew Chem Int Ed Engl 47:3688-94. 2008
    Bacterial acyltransferases are a new class of enzymes, and the first member was identified as WS/DGAT in Acinetobacter baylyi ADP1...
  38. doi Cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium Rhodococcus opacus PD630
    Adrian F Alvarez
    Centro Regional de Investigación y Desarrollo Científico Tecnológico CRIDECIT, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Comodoro Rivadavia, Argentina
    Microbiology 154:2327-35. 2008
    ..is a promiscuous acyltransferase (Atf), exhibiting acyl-CoA acyltransferase activity to both diacylglycerols (DGAT activity) and fatty alcohols (wax ester synthase, WS activity)...
  39. doi Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training
    Thomas J Alsted
    University of Copenhagen, The August Krogh Bldg 13, Universitetsparken, DK, 2100 Copenhagen, Denmark
    Am J Physiol Endocrinol Metab 296:E445-53. 2009
    ..No change was found in the expression of diacylglycerol acyl transferase 1 (DGAT1) after training...
  40. pmc Reduced capacity for fatty acid oxidation in rats with inherited susceptibility to diet-induced obesity
    Hong Ji
    Monell Chemical Senses Center, Philadelphia, PA 19104, USA
    Metabolism 56:1124-30. 2007
    ..These findings provide a metabolic mechanism for the development of diet-induced obesity and thus suggest potential targets for intervention strategies to treat or prevent it...
  41. ncbi Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis
    Jeong Yeh Yang
    Department of Animal and Dairy Science, 444 Edgar L Rhodes Center for Animal and Dairy Science, University of Georgia, Athens, GA 30602 2771, USA
    Apoptosis 12:1953-63. 2007
    ..such as PPARgamma, C/EBPalpha, and aP2 decreased after treatment with XN during the maturation period and that of DGAT1 decreased after treatment with XN and IXN...
  42. ncbi Amidepsines, inhibitors of diacylglycerol acyltransferase produced by Humicola sp. FO-2942. I. Production, isolation and biological properties
    H Tomoda
    Research Center for Biological Function, Kitasato Institute, Tokyo, Japan
    J Antibiot (Tokyo) 48:937-41. 1995
    ..FO-2942, a soil isolate, was found to produce a series of new inhibitors of diacylglycerol acyltransferase (DGAT)...
  43. ncbi Eicosapentaenoic and docosahexaenoic acid affect mitochondrial and peroxisomal fatty acid oxidation in relation to substrate preference
    L Madsen
    Department of Clinical Biochemistry, University of Bergen, Haukeland Hospital, Norway
    Lipids 34:951-63. 1999
    Decreased triacylglycerol synthesis within hepatocytes due to decreased diacylglycerol acyltransferase (DGAT) activity has been suggested to be an important mechanism by which diets rich in fish oil lower plasma triacylglycerol levels...
  44. ncbi Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members
    S Cases
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94143, USA
    J Biol Chem 276:38870-6. 2001
    ..DGAT2 is a member of a gene family that has no homology with DGAT1 and includes several mouse and human homologues that are candidates for additional DGAT genes...
  45. ncbi A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1
    Rainer Kalscheuer
    Institut fur Mikrobiologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, Germany
    J Biol Chem 278:8075-82. 2003
    ..acyltransferase is not related to known acyltransferases including the WS from jojoba (Simmondsia chinensis), the DGAT1 or DGAT2 families present in yeast, plants, and animals, and the phospholipid:diacylglycerol acyltransferase ..
  46. ncbi Expression pattern of diacylglycerol acyltransferase-1, an enzyme involved in triacylglycerol biosynthesis, in Arabidopsis thaliana
    Chaofu Lu Lu
    John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK
    Plant Mol Biol 52:31-41. 2003
    ..The expression pattern of the DGAT1 gene was studied in transgenic plants containing the reporter gene beta-glucuronidase (GUS) fused with DNA ..
  47. pmc The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase
    Tim Stöveken
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, D 48149 Munster, Germany
    J Bacteriol 187:1369-76. 2005
    The wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT) catalyzes the final steps in triacylglycerol (TAG) and wax ester (WE) biosynthesis in the gram-negative bacterium Acinetobacter sp. strain ADP1...
  48. ncbi DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle
    Chandra S Pareek
    Dept of Animal Genetics, Faculty of Animal Bio engineering, University of Warmia and Mazury, Oczapowskiego 5, 10 718 Olsztyn, Poland
    J Appl Genet 46:85-7. 2005
    The diacylglycerol o-acyltransferase 1 gene (DGAT1) was investigated in Polish Black-and-White cattle. The frequency of the K allele was 0.60, 0.68 and 0.48 for AI sires (n=150), young bulls (n=139) and cows (n=213), respectively...
  49. ncbi Plane of nutrition prepartum alters hepatic gene expression and function in dairy cows as assessed by longitudinal transcript and metabolic profiling
    Juan J Loor
    Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, USA
    Physiol Genomics 27:29-41. 2006
    ..Ad libitum feeding upregulated a number of genes associated with liver triacylglycerol synthesis (DGAT1) and proinflammatory cytokines (TNFAIP3)...
  50. ncbi Importance of PPAR alpha for the effects of growth hormone on hepatic lipid and lipoprotein metabolism
    Anna Ljungberg
    Wallenberg Laboratory for Cardiovascular Research, The Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
    Growth Horm IGF Res 17:154-64. 2007
    ..PPARalpha is activated by fatty acids and regulates hepatic lipid metabolism in rodents. The aim of this study was to investigate the importance of PPARalpha for the effects of GH on hepatic gene expression and lipoprotein metabolism...
  51. ncbi Generation of novel adipocyte monolayer cultures from embryonic stem cells
    Theresa L Chen
    Veterans Affairs, Palo Alto Health Care System, Palo Alto, CA 94304, USA
    Stem Cells Dev 16:371-80. 2007
    ..high levels of adipose-specific proteins (adipocyte markers), including PPARgamma2, ALBP, LPL, HSL, perilipin, and DGAT1. The adipocytes are functionally active, as evidenced by their response to lipolytic agents, such as forskolin, ..
  52. doi Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl CoA: diacylglycerol acyltransferase 1 (DGAT1)
    A Schennink
    Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
    J Dairy Sci 91:2135-43. 2008
    ..46 +/- 0.09 for C16 index. We genotyped the cows for the SCD1 A293V and DGAT1 K232A polymorphisms, which are known to alter milk fatty acid composition...
  53. pmc Retinol Esterification by DGAT1 Is Essential for Retinoid Homeostasis in Murine Skin
    Michelle Y S Shih
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    J Biol Chem 284:4292-9. 2009
    ..Here we show that the neutral lipid synthesis enzyme acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) functions as the major acyl-CoA:retinol acyltransferase (ARAT) in murine skin...
  54. doi Whole genome scan to detect quantitative trait loci for bovine milk protein composition
    G C B Schopen
    Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands
    Anim Genet 40:524-37. 2009
    ..protein composition could be explained by known polymorphisms in beta-casein, kappa-casein, beta-lactoglobulin and DGAT1 genes...
  55. doi Peroxisome proliferator-activated receptor-gamma activation and long-chain fatty acids alter lipogenic gene networks in bovine mammary epithelial cells to various extents
    A K G Kadegowda
    Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
    J Dairy Sci 92:4276-89. 2009
    ..intracellular lipid droplet formation and mRNA of ACSS2, LPIN1, SCD, and SREBF2 in response to 16:0, and greater DGAT1 and THRSP with 18:0...
  56. doi Osthol regulates hepatic PPAR alpha-mediated lipogenic gene expression in alcoholic fatty liver murine
    Fan Sun
    Department of Pharmacology, Medical College of Soochow University, Suzhou 215123, Jiangsu Province, China
    Phytomedicine 17:669-73. 2010
    ..The mRNA expressions of peroxisome proliferator-activated receptor (PPAR) alpha, diacylglycerol acyltransferase (DGAT), 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase and cholesterol 7 alpha-hydroxylase (CYP7A) in mouse hepatic ..
  57. doi Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle
    G Conte
    Dipartimento di Agronomia e Gestione dell Agroecosistema, University of Pisa, 56124, Italy
    J Dairy Sci 93:753-63. 2010
    ..acids (FA) in the cis-Delta(9) position in mammary glands of ruminant animals, diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in triacylglycerol synthesis in the mammary gland, and sterol regulatory element binding protein (..
  58. doi Characterization of DGAT1 allelic effects in a sample of North American Holstein cattle
    Marcos Vinicius G Barbosa da Silva
    Bovine Functional Genomics Laboratory, Agricultural Research Service, USDA, Beltsville, Maryland, USA
    Anim Biotechnol 21:88-99. 2010
    ..non-conservative substitution at amino acid 232 of the gene encoding the acylCoA:diacylglycerol acyltransferase (DGAT1) protein...
  59. doi Ionising radiation triggers fat accumulation in white adipose tissue
    Sung Kee Jo
    Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute ARTI, Korea Atomic Energy Research Institute KAERI, Jeongeup, Jeonbuk, Republic of Korea
    Int J Radiat Biol 87:302-10. 2011
    ..To investigate changes in gonadal white adipose tissue and lipogenesis-related gene expression induced by radiation exposure...
  60. doi Mifepristone treatment results in differential regulation of glycerolipid biosynthesis in baby hamster kidney cells expressing a mifepristone-inducible ABCA1
    Kristin D Hauff
    Department of Pharmacology and Therapeutics, Manitoba Institute of Child Health, Faculty of Medicine, University of Manitoba, 715 McDermot Avenue, Winnipeg, MB, R3E 0T6, Canada
    Lipids 46:795-804. 2011
    ..elevated only in mifepristone-induced ABCA1 expressing cells due to a decrease in diacylglycerol acyltransferase-1 (DGAT-1) activity...
  61. doi Polymorphisms in genes in the SREBP1 signalling pathway and SCD are associated with milk fatty acid composition in Holstein cattle
    Gonzalo Rincon
    Department of Animal Science, University of California, Davis, CA 95616, USA
    J Dairy Res 79:66-75. 2012
    ..b>DGAT1 K232A marker was included in the study to examine the effect of this marker on the variation of milk fatty acids ..
  62. doi Controlling lipid accumulation in cereal grains
    Guillaume Barthole
    Institut Jean Pierre Bourgin, UMR1318 INRA AgroParisTech, INRA Centre de Versailles Grignon, Route de Saint Cyr, F 78026 Versailles, France
    Plant Sci 185:33-9. 2012
    ..For instance, diacylglycerol acyltransferase (DGAT) that catalyses the ultimate reaction in the biosynthesis of triacylglycerol appears to be a promising target for ..
  63. doi Thiadiazoles as new inhibitors of diacylglycerol acyltransferase type 1
    Patrick Mougenot
    sanofi aventis R and D, Early to Candidate Unit, 1 Avenue Pierre Brossolette, 91385 Chilly Mazarin, France
    Bioorg Med Chem Lett 22:2497-502. 2012
    A novel class of DGAT1 inhibitors containing a thiadiazole core has been discovered. Chemical optimization lead to inhibitors of human DGAT1 with an appropriate ADME profile and that show in vivo activity in target tissues.
  64. doi Whole-genome association study for energy balance and fat/protein ratio in German Holstein bull dams
    J Tetens
    Institute of Animal Breeding and Husbandry, Christian Albrechts University, D 24118 Kiel, Hermann Rodewald Straße 6, Kiel, Germany
    Anim Genet 44:1-8. 2013
    ..associated with FPR were mostly located in QTL regions for milk production traits, especially in the region of DGAT1, whereas SNPs associated with EB mainly showed positional relationships to previously described QTL regions ..
  65. doi The atf2 gene is involved in triacylglycerol biosynthesis and accumulation in the oleaginous Rhodococcus opacus PD630
    Martín A Hernández
    Centro Regional de Investigación y Desarrollo Científico Tecnológico, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia, San Juan Bosco Km 4 Ciudad Universitaria 9000, Comodoro Rivadavia Chubut, Argentina
    Appl Microbiol Biotechnol 97:2119-30. 2013
    ..TAG biosynthesis is catalyzed by the bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (WS/DGAT) enzymes encoded by atf genes. R...
  66. pmc Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP
    Ashwin A Raut
    High Security Animal Disease Laboratory, Bhopal, Madhya Pradesh, India
    Genet Mol Biol 35:610-3. 2012
    Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle...
  67. pmc Exploring the genetic characteristics of two recombinant inbred line populations via high-density SNP markers in maize
    Qingchun Pan
    National Maize Improvement Center of China, China Agricultural University, Beijing, China
    PLoS ONE 7:e52777. 2012
    ..5 Mb to 1.72 Mb, and the QTL Oil6 (DGAT1-2 gene controlling oil concentration) was significantly reduced from 10.8 Mb to 1.62 Mb...
  68. ncbi Identification and validation of a selective small molecule inhibitor targeting the diacylglycerol acyltransferase 2 activity
    Mun Ock Kim
    Targeted Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon, Chungbuk 363 883, Korea
    Biol Pharm Bull 36:1167-73. 2013
    Diacylglycerol acyltransferase 2 (DGAT2) is one of two distinct DGAT enzymes that catalyze the last step in triacylglycerol (TG) synthesis...
  69. doi Genes involved in muscle lipid composition in 15 European Bos taurus breeds
    S Dunner
    Departamento de Produccion Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
    Anim Genet 44:493-501. 2013
    ..Several genes - ALDH2, CHRNE, CRHR2, DGAT1, IGFBP3, NEB, SOCS2, SUSP1, TCF12 and FOXO1 - also were found to be associated with both lipid and organoleptic ..
  70. doi Pharmacological inhibition of diacylglycerol acyltransferase 1 reduces body weight and modulates gut peptide release--potential insight into mechanism of action
    Jinqi Liu
    Merck Research Laboratories, Rahway, New Jersey, USA
    Obesity (Silver Spring) 21:1406-15. 2013
    Investigation was conducted to understand the mechanism of action of diacylglycerol acyltransferase 1 (DGAT1) using small molecules DGAT1 inhibitors, compounds K and L.
  71. pmc Palmitic acid and DGAT1 deficiency enhance osteoclastogenesis, while oleic acid-induced triglyceride formation prevents it
    Zoi Drosatos-Tampakaki
    Division of Periodontics, Columbia University College of Dental Medicine, New York, NY, USA
    J Bone Miner Res 29:1183-95. 2014
    ..Adenovirus-mediated expression of diacylglycerol acyl transferase 1 (DGAT1), a gene involved in triglyceride synthesis, also inhibits PA-induced osteoclastogenesis, suggesting a protective ..
  72. pmc Nannochloropsis genomes reveal evolution of microalgal oleaginous traits
    Dongmei Wang
    CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics and BioEnergy Genome Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
    PLoS Genet 10:e1004094. 2014
    ..The eleven type II diacylglycerol acyltransferase genes (DGAT-2) in every strain, each expressed during TAG synthesis, likely originated from three ancient genomes, including ..
  73. pmc Monoacylglycerol acyltransferase-2 is a tetrameric enzyme that selectively heterodimerizes with diacylglycerol acyltransferase-1
    Jun Zhang
    From the Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
    J Biol Chem 289:10909-18. 2014
    ..In this report, we investigated the tendency of MGAT2 to form an enzyme complex with DGAT1 and DGAT2 in intact cells...
  74. doi Discovery of 6-phenylpyrimido[4,5-b][1,4]oxazines as potent and selective acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) inhibitors with in vivo efficacy in rodents
    Brian M Fox
    Amgen Inc, 1120 Veterans Boulevard, South San Francisco, California 94080, United States
    J Med Chem 57:3464-83. 2014
    The discovery and optimization of a series of acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) inhibitors based on a pyrimido[4,5-b][1,4]oxazine scaffold is described...
  75. doi Recent advances in pharmacotherapy for hypertriglyceridemia
    Amirhossein Sahebkar
    Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Metabolic Research Centre, School of Medicine and Pharmacology, University of Western Australia, Perth, Australia
    Prog Lipid Res 56:47-66. 2014
    ..inhibitors of microsomal triglyceride transfer protein (MTTP) and acyl-CoA:diacylglycerol acyltransferase-1 (DGAT-1), incretin mimetics, and apolipoprotein (apo) B-targeted antisense oligonucleotides (ASOs) can all decrease the ..
  76. pmc Monoacylglycerol and diacylglycerol acyltransferases and the synthesis of neutral glycerides in Manduca sexta
    Jose L Soulages
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA Electronic address
    Insect Biochem Mol Biol 62:194-210. 2015
    ..The comparison suggested that insects may only have a single DGAT gene, DGAT1. The apparent absence of a DGAT2 gene in insects would represent a major difference with vertebrates, which ..
  77. doi Maternal restraint stress during pregnancy in mice induces 11β-HSD1-associated metabolic changes in the livers of the offspring
    H Maeyama
    1Department of Epigenetic Medicine, University of Yamanashi, Chuo, Yamanashi, Japan
    J Dev Orig Health Dis 6:105-14. 2015
    ..with increased expression of lipid metabolism-related proteins such as alanine aminotransferase 2 diglyceride acyltransferase 1, peroxisome proliferator-activated receptor gamma and glucocorticoid receptor...
  78. doi Effects of tung oilseed FAD2 and DGAT2 genes on unsaturated fatty acid accumulation in Rhodotorula glutinis and Arabidopsis thaliana
    Yicun Chen
    State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, People s Republic of China
    Mol Genet Genomics 290:1605-13. 2015
    ..The key enzymes fatty acid desaturase (FAD) and diacylglycerol acyltransferase (DGAT) are responsible for UFA biosynthesis (a push process) and assembling fatty acids into lipids (a pull process) in ..
  79. pmc Enhancing Cardiac Triacylglycerol Metabolism Improves Recovery From Ischemic Stress
    Stephen C Kolwicz
    Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA
    Diabetes 64:2817-27. 2015
    ..demonstrated that myosin heavy-chain-mediated cardiac-specific overexpression of diacylglycerol transferase 1 (MHC-DGAT1), the primary enzyme for TAG synthesis, preserved cardiac function in two lipotoxic mouse models despite ..
  80. doi Effect of casein genes - beta-LGB, DGAT1, GH, and LHR - on milk production and milk composition traits in crossbred Holsteins
    A Molee
    School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima Province, Thailand
    Genet Mol Res 14:2561-71. 2015
    ..gene family, including the beta-lactoglobulin gene (beta-LGB), acyl-CoA: diacylglycerol acyltransferase 1 gene (DGAT1), growth hormone gene (GH), and luteinizing hormone receptor gene (LHR) on milk yield, milk composition, the ..
  81. doi Discovery and optimization of adamantane carboxylic acid derivatives as potent diacylglycerol acyltransferase 1 inhibitors for the potential treatment of obesity and diabetes
    Suvarna H Pagire
    Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon 305 600, Republic of Korea Department of Medicinal and Pharmaceutical Chemistry, University of Science and Technology, 305 333, Republic of Korea
    Eur J Med Chem 101:716-35. 2015
    ..developed a series of adamantane carboxylic acid derivatives exhibiting potent diacylglycerol acyltransferase 1 (DGAT1) inhibitory activities...
  82. pmc Identification of genes coding for putative wax ester synthase/diacylglycerol acyltransferase enzymes in terrestrial and marine environments
    Mariana P Lanfranconi
    Centro Regional de Investigación y Desarrollo Científico Tecnológico CRIDECIT, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco y CIT CHUBUT CONICET, Km 4 Ciudad Universitaria, 9000, Comodoro Rivadavia, Chubut, Argentina
    AMB Express 5:128. 2015
    ..TAG) and wax esters (WE) is catalyzed in bacteria by wax ester synthase/diacylglycerol acyltransferase enzymes (WS/DGAT)...
  83. doi Type I Diacylglycerol Acyltransferase (MtDGAT1) from Macadamia tetraphylla: Cloning, Characterization, and Impact of Its Heterologous Expression on Triacylglycerol Composition in Yeast
    José María Arroyo-Caro
    Grupo de Biotecnología de Productos Naturales BIO 279, Centro de Investigación en Biotecnología Agroalimentaria BITAL, Campus Internacional de Excelencia Agroalimentario CeiA3, Universidad de Almeria, 04120 Almeria, Spain
    J Agric Food Chem 64:277-85. 2016
    ..In this work, a gene encoding a type 1 acyl-CoA:diacylglycerol acyltransferase (DGAT1) was cloned from M. tetraphylla...
  84. doi Metabolic engineering of enhanced glycerol-3-phosphate synthesis to increase lipid production in Synechocystis sp. PCC 6803
    Xi Wang
    Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164, USA
    Appl Microbiol Biotechnol 100:6091-101. 2016
    ..Heterologous genes that encoded glycerol-3-phosphate dehydrogenase (GPD) and diacylglycerol acyltransferase (DGAT) were engineered into Synechocystis sp. PCC 6803 to enhance G3P supply and lipid production...
  85. doi Genetic and environmental variation in bovine milk infrared spectra
    Qiuyu Wang
    Animal Breeding and Genomics Centre, Wageningen University, PO Box 338, 6700AH, Wageningen, The Netherlands Electronic address
    J Dairy Sci 99:6793-803. 2016
    ..20 to 0.60. The diacylglycerol O-acyltransferase 1 (DGAT1), stearoyl-CoA desaturase (SCD1), κ-casein (CSN3), and β-lactoglobulin (LGB) polymorphisms are known to have a ..
  86. doi Biochemical characterization of human acyl coenzyme A: 2-monoacylglycerol acyltransferase-3 (MGAT3)
    CURTIS BRANDT
    Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    Biochem Biophys Res Commun 475:264-70. 2016
    ..Furthermore, MGAT3 possesses both DGAT and MGAT activities, in vitro...
  87. pmc Acyl-CoA:Diacylglycerol Acyltransferase 1 Expression Level in the Hematopoietic Compartment Impacts Inflammation in the Vascular Plaques of Atherosclerotic Mice
    Nemanja Vujić
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria
    PLoS ONE 11:e0156364. 2016
    ..We have previously shown that ApoE-/-Dgat1-/- mice are protected from developing atherosclerosis in association with reduced foam cell formation...
  88. pmc Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism
    Vinay Sachdev
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, 8010 Graz, Austria
    Biochim Biophys Acta 1861:1132-41. 2016
    Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in triacylglycerol (TG) biosynthesis. Here we show that genetic deficiency and pharmacological inhibition of DGAT1 in mice alters cholesterol metabolism...
  89. ncbi Acrolein increases macrophage atherogenicity in association with gut microbiota remodeling in atherosclerotic mice: protective role for the polyphenol-rich pomegranate juice
    Oren Rom
    The Lipid Research Laboratory, Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Efron 1, Bat Galim, 31096, Haifa, Israel
    Arch Toxicol . 2016
    ..proteins (SREBPs), 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR), and diacylglycerol acyltransferase1 (DGAT1)...
  90. ncbi Diacylglycerol acyltransferase 1 (DGAT1) inhibition by furofuran lignans from stems of Acanthopanax senticosus
    Ban Ban Li
    College of Pharmacy, Beihua University, 3999 Binjiangdong Road, Jilin City, 132013, Jilin Province, China
    Arch Pharm Res . 2016
    ..All the isolates were evaluated for in vitro inhibitory activity against DGAT1 and DGAT2...
  91. doi Hepatic stellate cells retain the capacity to synthesize retinyl esters and to store neutral lipids in small lipid droplets in the absence of LRAT
    Mokrish Ajat
    Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, and Institute of Biomembranes, Utrecht University, P O Box 80176, 3508 TD Utrecht, The Netherlands
    Biochim Biophys Acta 1862:176-187. 2017
    ..To investigate the role of lecithin:retinol acyltransferase (LRAT) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) in retinyl ester synthesis and lipid droplet dynamics, we modified LC-MS/MS procedures by including multiple ..
  92. pmc Exendin-4 Inhibits Hepatic Lipogenesis by Increasing β-Catenin Signaling
    Mi Hae Seo
    Department of Internal Medicine, Soonchunhyang University Gumi Hospital, Gumi, Korea
    PLoS ONE 11:e0166913. 2016
    ..acetyl-CoA carboxylase (ACC) and triglyceride synthesis-involved genes, such as diacylglycerol acyltransferase 1 (DGAT1) and diacylglycerol acyltransferase 2 (DGAT2) in HepG2 cells, whereas exendin-4 treatment significantly prevented ..
  93. doi Adaptations to excess choline in insulin resistant and Pcyt2 deficient skeletal muscle
    Adrian Taylor
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada
    Biochem Cell Biol . 2016
    ..Choline reduced the expression of genes for FA and TAG formation (Scd1, Fas, Srebp1c, Dgat1/2), upregulated the genes for FA oxidation (Cpt1, Pparα, Pgc1α), and had minor effects on phospholipid and ..
  94. pmc Leptin modulates the effects of acyl CoA:diacylglycerol acyltransferase deficiency on murine fur and sebaceous glands
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    J Clin Invest 109:175-81. 2002
    ..Mice lacking DGAT1 (Dgat(-/-)) demonstrate significant changes in lipid metabolism in several tissues, including the skin...
  95. pmc Effect of 50 Hz electric field in diacylglycerol acyltransferase mRNA expression level and plasma concentration of triacylglycerol, free fatty acid, phospholipid and total cholesterol
    Takuya Hori
    Hakuju Institute for Health Science, Tokyo 151 0063, Japan
    Lipids Health Dis 11:68. 2012
    ..field (EF) on plasma level of triacylglycerol, free fatty acids, total cholesterol and phospholipid and mRNA expression level of diacylglycerol acyltransferase (DGAT) 1 and 2 in liver and intestines from C57BL/6 J mice were studied.
  96. pmc Gene expression in plant lipid metabolism in Arabidopsis seedlings
    An Shan Hsiao
    School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    PLoS ONE 9:e107372. 2014
    ..In both WS and Col-0, ACYL-COA-BINDING PROTEIN3 (ACBP3), DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and DGAT3 showed diurnal control in 2- and 5-day-old seedlings...
  97. pmc F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression
    Xiaoqing Mo
    Department of Endocrinology, First Affiliated Hospital, Sun Yat Sen University, No 58 Zhongshan 2nd road, Guangzhou 510080, PR China
    PLoS ONE 10:e0117156. 2015
    ..In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice...
  98. pmc Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight
    C Jako
    Seed Oil Biotechnology Group, National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan S7N 0W9, Canada
    Plant Physiol 126:861-74. 2001
    ..mutation is correlated with an altered seed fatty acid composition, reduced diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) activity, reduced seed triacylglycerol content, and delayed seed development in the AS11 mutant...
  99. ncbi Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice
    I J Waterman
    Cell Biochemistry, Hannah Research Institute, Ayr, Scotland, UK
    Int J Obes Relat Metab Disord 26:742-3. 2002
    ..Overt and latent hepatic DGAT activities for 16-week-old lean Ob/? mice were 0.42+/-0.14 and 1.57+/-0.04 micromol/mg/min, respectively...
  100. ncbi Dissociation of obesity and impaired glucose disposal in mice overexpressing acyl coenzyme a:diacylglycerol acyltransferase 1 in white adipose tissue
    Hubert C Chen
    Gladstone Institute of Cardiovascular Disease, San Francisco, California 94141 9100, USA
    Diabetes 51:3189-95. 2002
    Acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1) is one of two DGAT enzymes known to catalyze the final step in mammalian triglyceride synthesis...
  101. ncbi Transcriptional adaptations of lipid metabolism in tibialis anterior muscle of endurance-trained athletes
    Beat Schmitt
    Department of Anatomy, University of Bern, 3000 Bern 9, Switzerland
    Physiol Genomics 15:148-57. 2003
    ..muscle 6-phosphofructokinase, PFKM), intramyocellular storage of FA (diacylglycerol O-acyltransferase 1, DGAT), and beta-oxidation (long-chain acyl-coenzyme A dehydrogenase, ACADL) were invariant between TA of trained and ..

Research Grants27

  1. Role of DGAT1 in Mammary Gland Development and Cancer
    Sylvaine Cases; Fiscal Year: 2006
    ..the role of triacylglycerol (TG) metabolism in mammary epithelial development and breast cancer using mice lacking DGAT1 [unreadable] (Dgat1-/-), a major enzyme that synthesizes TGs from fatty acids and diacylglycerol substrates...
  2. IVAN PETER URAY; Fiscal Year: 2014
    ..The acyl-CoA diacylglcyerol acyl-transferase (DGAT1) enzyme catalyzes the formation of triglycerides by esterifying diacyl-glycerol with activated fatty acids...
  3. Cardiac metabolism and heart failure
    Rong Tian; Fiscal Year: 2010
    ..be achieved by overexpressing the rate-limiting enzyme for triglyceride synthesis: diacylglyerol acyltransferase (DGAT1)...
  4. Functional Analysis of MGAT Enzymes
    Robert V Farese; Fiscal Year: 2010
    ..We hypothesize that MGAT2, together with acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), which catalyzes the synthesis of TG from DG and fatty acyl CoA, is crucial for the absorption of dietary TG and ..
  5. Michael D Jensen; Fiscal Year: 2016
    ..acids via acyl-CoA synthetase (ACS);3) triglyceride (TG) synthesis -mediated by diacylglycerol acetyltransferase (DGAT) were poorly predictive of FFA storage, and thus almost certainly not rate limiting...
  6. SUNEIL KRISHNA KOLIWAD; Fiscal Year: 2014
    ..I showed that MCs with increased expression of acyl CoA: diacylglycerol acyltransferase 1 (DGAT1), an enzyme involved in TG synthesis (aP2-Dgat1), have increased TG storage capacity and resistance to FA-induced ..
  7. Robert V Farese; Fiscal Year: 2014
    ..Mammals possess two DGAT enzymes, DGAT1 and DGAT2, which differ substantially in their biochemical and physiological functions...
  8. DGAT1: Linking Fatty Acids to Inflammation and Metabolism in White Adipose Tissue
    SUNEIL KRISHNA KOLIWAD; Fiscal Year: 2013
    ..In addressing these, I developed a markedly improved proposal. I had aimed to explore how DGAT1 modulates metabolism in the white adipose tissue (WAT), and whether macrophages or adipocytes are dominant in this ..
  9. Alcohol Intake and Hepatic Fat Metabolism
    Li Shin Huang; Fiscal Year: 2012
    ..Specific Aim 2 will explore the specific roles that diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2), the two genetically distinct hepatic enzymes that catalyze the final step of TG synthesis, have in the ..
  10. Human B-cell metabolic signaling under chronic nutrient overload
    Guim Kwon; Fiscal Year: 2012
    ..be probed with rapamycin, an inhibitor of mTORC1;BN99, an inhibitor of acyl CoA:diacylglycerol acyltransferase 1 (DGAT1), an enzyme that catalyzes the final step of triglyceride (TG) biosynthesis;and SR-135, a peroxynitrite ..
  11. IRA JAY GOLDBERG; Fiscal Year: 2016
    ..from this grant were used to create mice with transgenic overexpression of diacylglycerol acyl transferase 1 (DGAT1) in heart and skeletal muscle, and to study animals with a genetic or pharmacologic deficiency of DGAT1...
  12. Genetic Determinants: Low HDL, High Triglycerides, Obes*
    Robert Mahley; Fiscal Year: 2003
    ..In Specific Aim 1, we will identify polymorphisms in acyl CoA:diacylglycerol acyltranferase (DGAT)- I and -2 and in ATP-binding cassette A I (ABCA I) genes that are associated with differences in BMI, HDL-C, and ..
  13. Mechanisms and Functions of Core-induced Lipid Droplet Accumulation in Hepatitis
    MELANIE MARIA OTT; Fiscal Year: 2010
    ..In cells lacking DGAT1 or treated with a DGAT1 inhibitor, core cannot induce lipid droplets and cannot localize to lipid droplets to ..
  14. IRA JAY GOLDBERG; Fiscal Year: 2015
    ..determine why cardiomyocyte specific loss of the triglyceride synthesis enzyme diacylglycerol acyl transferase 1 (DGAT1) causes accumulation of ceramide and diacylglycerol, and cardiac toxicity...
  15. DGAT1 Mediated Cardiac Steatosis
    DENIS J GLENN; Fiscal Year: 2013
    ..The research proposal seeks to understand the role of the enzyme, diacylglycerol acyltransferase 1 (DGAT1), which catalyzes the final step in triglyceride synthesis, in the heart...
  16. Robert V Farese; Fiscal Year: 2015
    ..We accomplish this by inducing acyl CoA: diacylglycerol acyltransferase (DGAT) 1 or DGAT2, which catalyze TG synthesis and lie just upstream to the process of LD formation...
  17. Flavonoids in the Amelioration of Hypertiglyceridemia
    Andre Theriault; Fiscal Year: 2004
    ..of hepatic microsomal triglyceride transfer protein (MTP) protein expression and diacylglycerol acyltransferase (DGAT) activity. These results are preliminary and need to be confirmed on a larger scale...
  18. Increased leptin sensitivity in Dgat -/- mice
    Hubert Chen; Fiscal Year: 2003
    ..sensitivity protect against obesity and diabetes? Studies of mice lacking acyI:CoA diacylglycerol acyltransferase (DGAT) may address these questions. DGAT catalyzes the final step in the major pathways of triglyceride synthesis...
  19. Fatty acid metabolism and DGAT1 deficiency
    CLAUDIO VILLANUEVA; Fiscal Year: 2006
    ..Mice lacking DGAT1, an enzyme involved in triacylglycerol synthesis, have increased energy expenditure and are therefore obesity ..
  20. Triglyceride Synthesis and Lipotoxicity
    Robert Farese; Fiscal Year: 2006
    ..Aim 1 describes the generation and analysis of mice that overexpress DGAT1 in skeletal muscle, pancreatic beta cells, and cardiac muscle...
  21. Molecular Analysis of Drosophila GABA Transporters
    Sandra Leal; Fiscal Year: 2003
    ..We hypothesize that alterations in GABA-mediated neurotransmission via perturbations in DGAT function will result in aberrant behaviors...
  22. MOLECULAR ANALYSIS OF THE DROSOPHILA GABA TRANSPORTERS
    Wendi Neckameyer; Fiscal Year: 2001
    ..This application proposes to (1) identify and pharmacologically characterize distinct members of the DGAT family, (2) identify their temporal and spatial expression to provide insight into their functional roles, and (3) ..
  23. Role of DGAT in Triacyglycerol Metabolism
    Robert V Farese; Fiscal Year: 2010
    ..Mammals possess two DGAT enzymes, DGAT1 and DGAT2, which differ substantially in their biochemical and physiological functions...
  24. The Role of DGAT1 In Muscle Lipotoxicity
    Yi Hao Yu; Fiscal Year: 2006
    ..the development of steatosis, we propose to develop two transgenic mice with tissue-specific overexpression of the Dgat1 (diacylglycerol acyltransferase-1, or DGAT1) gene...
  25. DGAT Overexpression In Mouse Adipose Tissue and Muscle
    Yi Hao Yu; Fiscal Year: 2006
    ..Two transgenic mouse models with tissue-specific overexpression of diacylglycerol acyl transferase (DGAT) will be studied. DGAT is a key enzyme in triglyceride (TG) synthesis...
  26. High fat feeding and intramyocellular lipid abnormality
    ZengKui Guo; Fiscal Year: 2005
    ..Thus, the proposed research will improve the understanding of the mechanism of insulin resistance and imcTG abnormalities in the obese rat that will benefit investigation of human obesity. ..
  27. LIPID METABOLISM IN FAT CELLS
    Wen Guo; Fiscal Year: 2010
    ..Together, these studies will provide new insights into the stress-induced regulation of lipogenesis in adipocytes, a potentially important avenue for modulating energy balance. ..