DEN1

Summary

Gene Symbol: DEN1
Description: SUMO/sentrin peptidase family member, NEDD8 specific
Alias: DEN1, NEDP1, PRSC2, sentrin-specific protease 8, NEDD8 specific-protease cysteine 2, NEDD8-specific protease 1, SUMO sentrin specific protease family member 8, SUMO/sentrin specific peptidase family member 8, deneddylase 1, protease, cysteine 2
Species: human

Top Publications

  1. ncbi Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity
    Dimitris P Xirodimas
    University of Dundee, Ninewells Hospital and Medical School, Department of Surgery and Molecular Oncology, Dundee DD1 9SY, UK
    Cell 118:83-97. 2004
  2. ncbi Short Communication: detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance
    V Thenmozhi
    Centre for Research in Medical Entomology, Indian Council of Medical Research, 4 Sarojini Street, Chinna Chokkikulam, Madurai 625 002, India
    Trop Med Int Health 10:187-9. 2005
  3. pmc Solution structure of the envelope protein domain III of dengue-4 virus
    David E Volk
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 1157, USA
    Virology 364:147-54. 2007
  4. doi Systematic in vivo RNAi analysis identifies IAPs as NEDD8-E3 ligases
    Meike Broemer
    The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, Mary Jean Mitchell Green Building, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
    Mol Cell 40:810-22. 2010
  5. pmc The molecular determinants of NEDD8 specific recognition by human SENP8
    Yung Cheng Shin
    Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan
    PLoS ONE 6:e27742. 2011
  6. ncbi DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
  7. pmc Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1
    Lin Nan Shen
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife, UK
    EMBO J 24:1341-51. 2005
  8. ncbi Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    J Mol Biol 345:141-51. 2005
  9. doi Chemotherapy induces NEDP1-mediated destabilization of MDM2
    I R Watson
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Oncogene 29:297-304. 2010
  10. ncbi Identification and characterization of DEN1, a deneddylase of the ULP family
    Tudeviin Gan-Erdene
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:28892-900. 2003

Research Grants

Scientific Experts

Detail Information

Publications76

  1. ncbi Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity
    Dimitris P Xirodimas
    University of Dundee, Ninewells Hospital and Medical School, Department of Surgery and Molecular Oncology, Dundee DD1 9SY, UK
    Cell 118:83-97. 2004
    ....
  2. ncbi Short Communication: detection of dengue virus antigens in desiccated mosquitoes: an improved tool for surveillance
    V Thenmozhi
    Centre for Research in Medical Entomology, Indian Council of Medical Research, 4 Sarojini Street, Chinna Chokkikulam, Madurai 625 002, India
    Trop Med Int Health 10:187-9. 2005
    ..We tested the effect of storage on the stability of DEN1, DEN2, DEN3 and DEN4 antigens...
  3. pmc Solution structure of the envelope protein domain III of dengue-4 virus
    David E Volk
    Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555 1157, USA
    Virology 364:147-54. 2007
    The disease dengue (DEN) is caused by four serologically related viruses termed DEN1, DEN2, DEN3 and DEN4. The structure of the ectodomain of the envelope protein has been determined previously for DEN2 and DEN3 viruses...
  4. doi Systematic in vivo RNAi analysis identifies IAPs as NEDD8-E3 ligases
    Meike Broemer
    The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, Mary Jean Mitchell Green Building, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK
    Mol Cell 40:810-22. 2010
    ..Consistent with the notion that attachment of NEDD8 prevents cell death, genetic ablation of deneddylase 1 (DEN1) suppresses apoptosis...
  5. pmc The molecular determinants of NEDD8 specific recognition by human SENP8
    Yung Cheng Shin
    Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, Taiwan
    PLoS ONE 6:e27742. 2011
    ..However, USP2 cannot cleave the N51E/A72R double mutant of the CrNEDD8 subdomain, suggesting that USP2 requires additional recognition sites...
  6. ncbi DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
    ..Here we report the isolation, cloning, and characterization of a novel human Nedd8-specific protease called DEN1. Human DEN1 is encoded by AAH31411...
  7. pmc Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1
    Lin Nan Shen
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife, UK
    EMBO J 24:1341-51. 2005
    NEDD8 (neural precursor cell expressed developmentally downregulated gene 8)-specific protease NEDP1 processes preNEDD8 to its mature form and deconjugates NEDD8 from substrates such as p53 and cullins...
  8. ncbi Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1
    David Reverter
    Structural Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
    J Mol Biol 345:141-51. 2005
    ..We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state ..
  9. doi Chemotherapy induces NEDP1-mediated destabilization of MDM2
    I R Watson
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Oncogene 29:297-304. 2010
    ..Here, we show that the stability of MDM2 is regulated by an ubiquitin-like NEDD8 pathway and identify NEDP1 as a chemotherapy-induced isopeptidase that deneddylates MDM2, resulting in MDM2 destabilization concomitant with ..
  10. ncbi Identification and characterization of DEN1, a deneddylase of the ULP family
    Tudeviin Gan-Erdene
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:28892-900. 2003
    ..In contrast, a second and more selective enzyme is labeled only by Nedd8 vinyl sulfone. This protein, DEN1, is a 221-amino acid thiol protease that is encoded by an open reading frame previously annotated as SENP8...
  11. ncbi NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins
    Heidi M Mendoza
    Centre for Biomolecular Sciences, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY169AL, United Kingdom
    J Biol Chem 278:25637-43. 2003
    ..Here we identify and characterize NEDP1, a human NEDD8-specific protease...
  12. doi The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis
    Ping Xie
    1 State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Collaborative Innovation Center for Cancer Medicine, Beijing 100850, China 2 Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning Province 116044, China 3
    Nat Commun 5:3733. 2014
    ..These findings provide evidence that neddylation is important in HECT ubiquitin ligase activation and shed new light on the tumour-promoting role of Smurf1. ..
  13. pmc DENEDDYLASE1 counters automodification of neddylating enzymes to maintain NEDD8 homeostasis in Arabidopsis
    Julia Mergner
    Technical University of Munich, Germany
    J Biol Chem . 2017
    ..We previously characterized Arabidopsis thaliana mutants with defects in the conserved NEDD8-specific protease DEN1 (DENEDDYLASE1)...
  14. doi MyD88 NEDDylation negatively regulates MyD88-dependent NF-κB signaling through antagonizing its ubiquitination
    Fangxue Yan
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    Biochem Biophys Res Commun 482:632-637. 2017
    ..We also show that deNEDDylase NEDP1 serves as a regulator of this process...
  15. pmc A New Method for Identifying Essential Proteins Based on Network Topology Properties and Protein Complexes
    Chao Qin
    Beijing Key Lab of Traffic Data Analysis and Mining, Beijing Jiaotong University, Beijing, China
    PLoS ONE 11:e0161042. 2016
    ..First, we introduce the common centrality measures; second, we propose the densities Den1(v) and Den2(v) of a node v to describe its local properties in the network; and finally, the combined strategy of ..
  16. doi Computational prediction and experimental characterization of a "size switch type repacking" during the evolution of dengue envelope protein domain III (ED3)
    Montasir Elahi
    Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Nakamachi, Koganei Shi, Tokyo 184 8588, Japan
    Biochim Biophys Acta 1844:585-92. 2014
    Dengue viruses (DEN) are classified into four serotypes (DEN1-DEN4) exhibiting high sequence and structural similarities, and infections by multiple serotypes can lead to the deadly dengue hemorrhagic fever...
  17. doi Deneddylase 1 regulates deneddylase activity of the Cop9 signalosome in Drosophila melanogaster
    Kiyoung Kim
    Department of Medical Biotechnology, Soonchunhyang University, Asan, 336 745
    Insect Sci 24:27-34. 2017
    ..Another deneddylase, Deneddylase 1, has also been shown to process the Nedd8 precursor...
  18. pmc Dengue serosurvey in Sint Eustatius
    Teresa Leslie
    Department of Anthropology, University of Maryland, College Park, Maryland, United States of America Eastern Caribbean Public Health Foundation, Sint Eustatius, Netherlands Dutch Caribbean
    PLoS ONE 9:e95002. 2014
    ..The lack of antibodies to the DEN1, 3, and 4 in the samples collected from participants under 20 years of age suggests that only DEN2 has circulated ..
  19. pmc Stabilization of HIF through inhibition of Cullin-2 neddylation is protective in mucosal inflammatory responses
    Valerie F Curtis
    Mucosal Inflammation Program, Department of Medicine, and
    FASEB J 29:208-15. 2015
    ..Pharmacologic inhibition of cullin neddylation may provide a therapeutic opportunity in mucosal inflammatory disease...
  20. pmc The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development
    Josua Schinke
    Department of Molecular Microbiology and Genetics, Göttingen Center for Molecular Biosciences GZMB, and Georg August University, Gottingen, Germany
    PLoS Genet 12:e1005949. 2016
    DenA/DEN1 and the COP9 signalosome (CSN) represent two deneddylases which remove the ubiquitin-like Nedd8 from modified target proteins and are required for distinct fungal developmental programmes...
  21. doi Preclinical and clinical development of a dengue recombinant subunit vaccine
    Susan B Manoff
    Merck and Co, Inc, Kenilworth, NJ, United States
    Vaccine 33:7126-34. 2015
    ..A monovalent version of the vaccine candidate, DEN1-80E, was formulated with Alhydrogel™ and studied in a proof-of-principle Phase I clinical trial by Hawaii Biotech,..
  22. pmc DENEDDYLASE1 deconjugates NEDD8 from non-cullin protein substrates in Arabidopsis thaliana
    Julia Mergner
    Plant Systems Biology, Technische Universitat Munchen, 85354 Freising, Germany Proteomics and Bioanalytics, Technische Universitat Munchen, 85354 Freising, Germany
    Plant Cell 27:741-53. 2015
    ..In animals and yeast, DENEDDYLASE1 (DEN1) processes NEDD8...
  23. doi Preclinical development of a dengue tetravalent recombinant subunit vaccine: Immunogenicity and protective efficacy in nonhuman primates
    Dhanasekaran Govindarajan
    Merck Research Laboratories, Merck and Co, Inc, Kenilworth, NJ, USA
    Vaccine 33:4105-16. 2015
    ..A similar study was conducted to evaluate tetravalent formulations at low (3, 3, 3, 6 μg of DEN1-80E, DEN2-80E, DEN3-80E and DEN4-80E respectively), medium (10, 10, 10, 20 μg) and high (50, 50, 50, 100 μg) ..
  24. doi Structural and biophysical analysis of sero-specific immune responses using epitope grafted Dengue ED3 mutants
    Manjiri R Kulkarni
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184 8588, Japan
    Biochim Biophys Acta 1854:1438-43. 2015
    ..is a re-emerging tropical disease and its severe form is caused by cross-reactivity between its four serotypes (DEN1, DEN2, DEN3 and DEN4)...
  25. ncbi Deep vein thrombosis associated with dengue fever
    Amrita Roy
    Department of Pediatrics, Medical College and Hospitals, Kolkata, India Correspondence to Dr Jasodhara Chaudhuri, 247 Lake Gardens, Kolkata 700 045, West Bengal, India
    Indian Pediatr 50:1053-4. 2013
    ..Hemorrhagic manifestations are common with Dengue but thrombotic events are uncommonly reported...
  26. doi hCINAP is a novel regulator of ribosomal protein-HDM2-p53 pathway by controlling NEDDylation of ribosomal protein S14
    J Zhang
    1 State Key Lab of Protein and Plant Gene Research, Beijing, China 2 Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing, China
    Oncogene 33:246-54. 2014
    ..These findings suggest that hCINAP is an important regulator of RP-HDM2-p53 pathway and a potential anticancer drug target. ..
  27. pmc ElaD, a Deubiquitinating protease expressed by E. coli
    Andre Catic
    Immunology Program, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 2:e381. 2007
    ..One particular set of cysteine proteases, the CE clan, which targets ubiquitin and Ubls, has homologs in eukaryotes, prokaryotes, and viruses...
  28. doi A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases
    Stefano Gastaldello
    Department of Cell and Molecular Biology, Karolinska Institutet, Box 285, S 17177 Stockholm, Sweden
    Nat Cell Biol 12:351-61. 2010
    ..Homologues encoded by other herpesviruses share the deneddylase activity. Thus, these enzymes are likely to have a key function in the virus life cycle by inducing a replication-permissive S-phase-like cellular environment...
  29. ncbi Clinical differences among PCR-proven dengue serotype infections
    Kriengsak Limkittikul
    Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 36:1432-8. 2005
    ..Their ages ranged from 3-30 years. They were infected with DEN1, DEN2, DEN3 and DEN4 in 21, 55, 12, and 12%, respectively...
  30. ncbi Identification and characterization of carbohydrate molecules in mammalian cells recognized by dengue virus type 2
    Chie Aoki
    Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Core Research for Evolutional Science and Technology CREST, Japan Science and Technology Corporation, and COE Program in the 21st Century
    J Biochem 139:607-14. 2006
    ..All serotypes of DEN viruses, DEN1 to DEN4, reacted with nLc4Cer, and the non-reducing terminal disaccharide residue Galbeta1-4GlcNAcbeta1- was found ..
  31. ncbi In vitro cleavage of Nedd8 from cullin 1 by COP9 signalosome and deneddylase 1
    Kosj Yamoah
    Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    Methods Enzymol 398:509-22. 2005
    ..This chapter provides methods for the preparation of two Nedd8 isopeptidases: the COP9 signalosome and human deneddylase 1. It also describes the development of cell-free systems for cleavage of the Nedd8-cullin 1 isopeptide bond ..
  32. pmc Regulation of parkin and PINK1 by neddylation
    Yeun Su Choo
    Sanford Burnham Medical Research Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Hum Mol Genet 21:2514-23. 2012
    ..Together, these results suggest that parkin and PINK1 are regulated by neddylation and that impaired NEDD8 modification of these proteins likely contributes to PD pathogenesis...
  33. doi NEDDylation controls the target specificity of E2F1 and apoptosis induction
    I Aoki
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Oncogene 32:3954-64. 2013
    ..These results suggest that NEDDylation is a molecular trigger that modifies the target specificity of E2F1, and could have important implications for E2F1 regulation of apoptosis...
  34. pmc Central role for endothelial human deneddylase-1/SENP8 in fine-tuning the vascular inflammatory response
    Stefan F Ehrentraut
    Mucosal Inflammation Program, Department of Medicine and Immunology, University of Colorado School of Medicine, Aurora, CO 80045, USA
    J Immunol 190:392-400. 2013
    ..Moreover, our findings provide feasibility for therapeutic targeting of the Culs during inflammation...
  35. ncbi Chimeric dengue type 2/type 1 viruses induce immune responses in cynomolgus monkeys
    Siritorn Butrapet
    Center for Vaccine Development, Institute of Science and Technology for Research and Development, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 33:589-99. 2002
    ..These DEN2/1 viruses induce high levels of DEN1 virus-specific neutralizing antibodies in mice...
  36. pmc Control of multicellular development by the physically interacting deneddylases DEN1/DenA and COP9 signalosome
    Martin Christmann
    Department of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics, Georg August Universitat Gottingen, Gottingen, Germany
    PLoS Genet 9:e1003275. 2013
    ..The DEN1/DenA and the COP9 signalosome (CSN) deneddylases physically interact in A...
  37. ncbi Replication of chimeric yellow fever virus-dengue serotype 1-4 virus vaccine strains in dendritic and hepatic cells
    Samantha Brandler
    Institute of Virology, Medical University of Vienna, Vienna, Austria
    Am J Trop Med Hyg 72:74-81. 2005
    ..In this study, the growth kinetics of ChimeriVax-DEN1-4 and parent viruses (wild-type DEN-1-4 and YF 17D) were assessed in human myeloid dendritic cells (DCs) and in ..
  38. pmc Myeloma overexpressed 2 (Myeov2) regulates L11 subnuclear localization through Nedd8 modification
    Manato Ebina
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
    PLoS ONE 8:e65285. 2013
    ..with a Nedd8 deconjugation enzyme COP9 signalosome, L11 deneddylation was mediated by another deneddylase Nedp1, independently of Myeov2. Finally, p53 transcriptional activity is upregulated by Myeov2 expression...
  39. pmc Serotype-specific structural differences in the protease-cofactor complexes of the dengue virus family
    Sumana Chandramouli
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    J Virol 84:3059-67. 2010
    ..additional challenges due to the occurrence of four antigenically distinct serotypes of the causative dengue virus (DEN1 to -4)...
  40. doi Age-specific prevalence of dengue antibodies in Bangkok infants and children
    Krisana Pengsaa
    Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
    Pediatr Infect Dis J 27:461-3. 2008
    ..b>DEN1 was the most prevalent, followed by DEN3, DEN2, and DEN4...
  41. doi Dengue-4 envelope domain III fused twice within the meningococcal P64k protein carrier induces partial protection in mice
    Laura Lazo
    Center for Genetic Engineering and Biotechnology PO Box 6162, Havana 10 600, Cuba
    Biotechnol Appl Biochem 52:265-71. 2009
    ..the protective capacity, in a viral encephalitis murine model, of fusion protein P64k-envelope domain III of DEN1, DEN2 and DEN3...
  42. doi DeSUMOylating enzymes--SENPs
    Marcin Drag
    Program in Apoptosis and Cell Death Research, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    IUBMB Life 60:734-42. 2008
    ..We discuss recent progress on the human SENPs and their substrates...
  43. doi DEN1 deneddylates non-cullin proteins in vivo
    Yaru Chan
    Institute of Molecular Biology, Academia Sinica, 128 Sec No 2 Academia Road, Taipei 115, Taiwan
    J Cell Sci 121:3218-23. 2008
    ..The cysteine protease DEN1/NEDP1 specifically processes the Nedd8 precursor and has been suggested to deconjugate Nedd8 from cullin proteins...
  44. doi Dengue virus regulates type I interferon signalling in a strain-dependent manner in human cell lines
    Indira Umareddy
    Novartis Institute for Tropical Diseases, 10, Biopolis Road, 05 01 Chromos, 138670, Singapore
    J Gen Virol 89:3052-62. 2008
    ..MY22713 (DEN4) could suppress the IFN response in a similar fashion to NGC, three other strains of dengue [EDEN167 (DEN1), MY02569 (DEN1) and MY10340 (DEN2)] were unable to suppress the IFN response, suggesting that this difference is ..
  45. doi A time-resolved fluorescence resonance energy transfer-based assay for DEN1 peptidase activity
    Ingo H Engels
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Anal Biochem 390:85-7. 2009
    ..It thereby controls key events in cell cycle progression and embryogenesis. Deneddylase-1 (DEN1/NEDP1/SENP8) features a selective peptidase activity converting the proNEDD8 precursor to its mature form and an ..
  46. ncbi Flow cytometric analysis of dengue virus-infected cells in peripheral blood
    Michael O Baclig
    Research and Biotechnology Division, St Luke s Medical Center, Quezon City, Philippines
    Southeast Asian J Trop Med Public Health 41:1352-8. 2010
    ..Virus isolation and serotype-specific RT-PCR revealed the cells were infected with dengue serotype 3 (DEN3). Our results suggest B cells may play an important role in DEN1 and DEN3 replication, and dissemination in vivo.
  47. pmc A single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys
    F Guirakhoo
    Acambis, Inc, 38 Sidney St, Cambridge, MA 02139, USA
    J Virol 78:9998-10008. 2004
    A chimeric yellow fever-dengue 1 (ChimeriVax-DEN1) virus was produced by the transfection of Vero cells with chimeric in vitro RNA transcripts...
  48. ncbi The antigenic structure of dengue type 1 virus envelope and NS1 proteins expressed in Escherichia coli
    P W Mason
    Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, Connecticut 06510
    J Gen Virol 71:2107-14. 1990
    The antigenic structures of the envelope protein, E, and the non-structural protein, NS1, of dengue type 1 virus (DEN1) have been studied in the form of recombinant fusion proteins expressed in Escherichia coli...
  49. ncbi Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein
    T Cui
    Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore
    Virology 246:409-17. 1998
    ..of the purified fusion protein with thrombin released a 68-kDa protein which is the expected molecular mass for the DEN1 NS3 protein. The identity of this protein was confirmed by Western blotting using dengue virus antisera...
  50. ncbi Evaluation of molecular strategies to develop a live dengue vaccine
    C J Lai
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Clin Diagn Virol 10:173-9. 1998
    ..Mortality is low but infection can lead to a severe form of dengue, characterised by haemorrhage and shock. A safe and effective vaccine against dengue is still not available...
  51. ncbi Construction of a full length infectious clone for dengue-1 virus Western Pacific,74 strain
    B Puri
    Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20889 5607, USA
    Virus Genes 20:57-63. 2000
    The flavivirus dengue 1 Western Pacific,74 (DEN1 WP) virus has a positive-stranded RNA genome of 10,735 nucleotides. DEN1 WP genomic RNA was amplified into three overlapping fragments by RT-PCR...
  52. ncbi First isolation of dengue 1 virus from Aedes aegypti in Federal District, Brazil
    N Degallier
    IRD, Brasilia, DF, 71619 970, Brasil
    Rev Soc Bras Med Trop 33:95-6. 2000
    ..Two strains of DEN1 were isolated from 9 pools of female Aedes aegypti (78 mosq...
  53. pmc Construction, safety, and immunogenicity in nonhuman primates of a chimeric yellow fever-dengue virus tetravalent vaccine
    F Guirakhoo
    Acambis, Inc, Cambridge, Massachusetts 02139, USA
    J Virol 75:7290-304. 2001
    ..additional YF/DEN chimeras using premembrane (prM) and envelope (E) genes of wild-type (WT) clinical isolates: DEN1 (strain PUO359, isolated in 1980 in Thailand), DEN3 (strain PaH881/88, isolated in 1988 in Thailand), and DEN4 (..
  54. pmc Derivation and characterization of a dengue type 1 host range-restricted mutant virus that is attenuated and highly immunogenic in monkeys
    Lewis Markoff
    Laboratory of Vector Borne Virus Diseases, Office of Vaccines Research and Review, Division of Veterinary Services, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Virol 76:3318-28. 2002
    ..into the analogous region of an infectious DNA derived from the genome of a human-virulent dengue serotype 1 virus (DEN1), strain Western Pacific (DEN1WP)...
  55. ncbi Inhibition of viral gene expression and replication in mosquito cells by dsRNA-triggered RNA interference
    Natasha J Caplen
    National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA
    Mol Ther 6:243-51. 2002
    ..and replication, we used two mosquito-borne viruses, Semliki Forest virus (SFV) and the serotype 1 dengue virus (DEN1)...
  56. pmc A live, attenuated dengue virus type 1 vaccine candidate with a 30-nucleotide deletion in the 3' untranslated region is highly attenuated and immunogenic in monkeys
    Stephen S Whitehead
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:1653-7. 2003
    ..the 3' untranslated region (3' UTR), was introduced into a homologous region of wild-type (wt) dengue virus type 1 (DEN1)...
  57. ncbi Aedes albopictus may not be vector of dengue virus in human epidemics in Brazil
    Nicolas Degallier
    Laboratorio Central, Secretaria Estadual da Saúde, Brasilia, DF, Brazil
    Rev Saude Publica 37:386-7. 2003
    ..Of 37 Ae. aegypti and 200 Ae. albopictus adult mosquitoes collected and inoculated, DEN1 virus was isolated only from a pool of two Ae. aegypti female mosquitoes. The study results suggest that Ae...
  58. pmc Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins
    Joris Hemelaar
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 24:84-95. 2004
    ..We identify Ub C-terminal hydrolase L1 (UCH-L1) and DEN1/NEDP1/SENP8, in addition to UCH-L3, as proteases with specificity for Nedd8...
  59. ncbi Diethylnitrosamine may induce esophageal dysplasia after local intramural administration
    W Zgodzinski
    2nd Department of General Surgery, Medical University, Lublin, Poland
    Rocz Akad Med Bialymst 48:48-51. 2003
    ..To assess the possibility of promoting esophageal squamous cell carcinoma after direct administration of diethylnitrosamine (DEN) into the wall of the esophagus...
  60. pmc Safety and efficacy of chimeric yellow Fever-dengue virus tetravalent vaccine formulations in nonhuman primates
    F Guirakhoo
    Acambis, Inc, Cambridge, Massachusetts 02139, USA
    J Virol 78:4761-75. 2004
    ..ChimeriVax-DEN1-4 vaccine viruses were prepared by electroporation of Vero cells with RNA transcripts prepared from viral cDNA (F...
  61. ncbi En1 and Wnt7a interact with Dkk1 during limb development in the mouse
    Maja Adamska
    Department of Human Genetics, University of Michigan, Ann Arbor 48109 0618, USA
    Dev Biol 272:134-44. 2004
    ..Reducing Dkk1 levels in Dkk1d/dEn1-/- double mutants resulted in severe phenotypes not seen in either single mutant, including fused bones in the ..
  62. ncbi Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs
    Jowita Mikolajczyk
    Program in Apoptosis and Cell Death Research, Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Biol Chem 282:26217-24. 2007
    ..Using these synthetic substrates we reveal that the SUMO domain enhances catalysis of SENP1, -2, -5, -6, and -7, demonstrating substrate-induced activation of SENPs by SUMOs...
  63. ncbi [Detection of flaviviruses by reverse-transcription polymerase chain reaction with universal primer set]
    M Fang
    Military Medical Institute, Guangzhou Military District, Department of Microbiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510507
    Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 11:267-70, 273. 1997
    ..Another five different internal primers were selected on the NS1 genes of DEN1, 2, 3, 4 and JEV...
  64. ncbi Diagnosis of dengue infection using various diagnostic tests in the early stage of illness
    Rangsima Lolekha
    Department of Pediatrics, Faculty of Medicine at Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Southeast Asian J Trop Med Public Health 35:391-5. 2004
    ..Viral serotypes identified by viral isolation, and RT-PCR were concordant: DEN1 was isolated in 8, DEN2 in 13, DEN3 in 5, and DEN4 in 2 patients...
  65. pmc Recombinant, live-attenuated tetravalent dengue virus vaccine formulations induce a balanced, broad, and protective neutralizing antibody response against each of the four serotypes in rhesus monkeys
    Joseph E Blaney
    Laboratory of Infectious Diseases, NIH, NIAID, LID Twinbrook III, Room 3W 13, 12735 Twinbrook Parkway, MSC 8133, Bethesda, MD 20892 8133, USA
    J Virol 79:5516-28. 2005
    ..TV-1 consists of recombinant DEN1, -2, -3, and -4, each with a 30-nucleotide deletion in the 3' untranslated region (Delta30)...
  66. ncbi Safety testing for neurovirulence of novel live, attenuated flavivirus vaccines: infant mice provide an accurate surrogate for the test in monkeys
    Thomas P Monath
    Acambis Inc, 38 Sidney Street, Cambridge, MA 02139, USA
    Biologicals 33:131-44. 2005
    ..The test in suckling mice discriminated between strains of two different vaccines (ChimeriVax-JE and ChimeriVax-DEN1) differing by a single amino acid change, and was more sensitive for detecting virulence differences than the test ..
  67. ncbi Non-structural proteins of dengue 2 virus offer limited protection to interferon-deficient mice after dengue 2 virus challenge
    Amanda E Calvert
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention CDC, Public Health Service, US Department of Health and Human Services, PO Box 2087, Fort Collins, CO 80522, USA
    J Gen Virol 87:339-46. 2006
    ..AG129 interferon-deficient mice, previously shown to be protected against lethal DEN1 or DEN2 viral infection after vaccination with a wild-type or candidate vaccine strain of DEN1 or DEN2 virus, ..
  68. ncbi Development of a live attenuated dengue virus vaccine using reverse genetics
    Joseph E Blaney
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, LID, Bethesda, Maryland 20892 8133, USA
    Viral Immunol 19:10-32. 2006
    There are four serotypes of dengue (DEN1-DEN4) virus that are endemic in most areas of Southeast Asia, Central and South America, and other subtropical regions...
  69. ncbi Mdm2-mediated NEDD8 modification of TAp73 regulates its transactivation function
    Ian R Watson
    Cancer Research Program and Division of Haematology Oncology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    J Biol Chem 281:34096-103. 2006
    ..Co-expression of NEDP1, a cysteine protease that specifically cleaves NEDD8 conjugates, was shown to deneddylate TAp73...
  70. ncbi Neddylation of a breast cancer-associated protein recruits a class III histone deacetylase that represses NFkappaB-dependent transcription
    Fei Gao
    Department of Cardiology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 449, Houston, TX 77030, USA
    Nat Cell Biol 8:1171-7. 2006
    ..These results provide a crucial link between neddylation and transcriptional regulation by SIRT1, a NAD-dependent histone deacetylase that prolongs life span in yeast and worms...
  71. ncbi The live attenuated dengue serotype 1 vaccine rDEN1Delta30 is safe and highly immunogenic in healthy adult volunteers
    Anna P Durbin
    Center for Immunization Research, Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
    Hum Vaccin 2:167-73. 2006
    The live attenuated DEN1 vaccine candidate virus rDEN1Delta30 has been evaluated in preclinical animal models and found to be attenuated and immunogenic...
  72. pmc Vaccine candidates for dengue virus type 1 (DEN1) generated by replacement of the structural genes of rDEN4 and rDEN4Delta30 with those of DEN1
    Joseph E Blaney
    Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Virol J 4:23. 2007
    ..safety and immunogenicity of rDEN2/4Delta30(ME) in humans, we now describe the generation of a panel of four antigenic chimeric DEN4 viruses using either the capsid (C), M, and E (CME) or ME structural genes of DEN1 Puerto Rico/94 strain.
  73. ncbi Full-length cDNA sequence of dengue type 1 virus (Singapore strain S275/90)
    J Fu
    Institute of Molecular and Cell Biology, Faculty of Medicine, National University of Singapore, Lower Kent Ridge
    Virology 188:953-8. 1992
    ..Both the nucleotide and deduced amino acid sequences were compared with the partial sequence of DEN1 (Nauru Island) and complete sequences of DNE2 (Jamaica), DEN3 (H87), and DEN4 (Dominica) virus genomes.
  74. ncbi Induction of bivalent immune responses by expression of dengue virus type 1 and type 2 antigens from a single complex adenoviral vector
    Nicholas U Raja
    Division of Biodefense Vaccines, GenPhar Incorporated, Mount Pleasant, South Carolina 29464, USA
    Am J Trop Med Hyg 76:743-51. 2007
    ..There are four serotypes of dengue virus (DEN1-4), and immunity to one serotype does not cross protect from infection with other serotypes...
  75. ncbi Burden of symptomatic dengue infection in children at primary school in Thailand: a prospective study
    Katie B Anderson
    Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Lancet 369:1452-9. 2007
    ..Dengue viruses are a major cause of morbidity and mortality in tropical and subtropical areas. Our aim was to assess prospectively the burden of dengue-related illness in children in Thailand...
  76. ncbi Induction of tetravalent protective immunity against four dengue serotypes by the tandem domain III of the envelope protein
    Shuiping Chen
    State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, People s Republic of China
    DNA Cell Biol 26:361-7. 2007
    ..sera from mice immunized with the purified protein were confirmed to contain specific high antibody titers against DEN1, DEN2, and DEN4, and moderate antibody titer against DEN3...

Research Grants4

  1. Identification and Analysis of Flavivirus Protease and RNA Helicase Inhibitors
    Radhakrishnan Padmanabhan; Fiscal Year: 2009
    ..of Class A, B, or C emerging human pathogens such as Yellow fever virus (YFV), West Nile virus (WNV) and Dengue (DEN1-4) viruses that cause serious illnesses with considerable morbidity and mortality...
  2. MOLECULAR EPIDEMIOLOGY OF DENGUE IN PUERTO RICO
    W McMillan; Fiscal Year: 2002
    ..There are four well-characterized dengue serotypes, DEN1-4, that are currently circulating globally...
  3. Selective Allosteric Inhibitors of SENP8
    Guy S Salvesen; Fiscal Year: 2012
    ....
  4. LIVE, ATTENUATED, RECOMBINANT VACCINE - WEST NILE VIRUS
    Thomas Monath; Fiscal Year: 2002
    ..abstract_text> ..