cytochrome b5 reductase

Summary

Gene Symbol: cytochrome b5 reductase
Description: cytochrome b5 reductase 3
Alias: B5R, DIA1, NADH-cytochrome b5 reductase 3, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, diaphorase-1, mutant NADH-cytochrome b5 reductase
Species: human

Top Publications

  1. ncbi Soluble cytochrome b 5 reductase from human erythrocytes
    P G Passon
    Biochim Biophys Acta 275:62-73. 1972
  2. ncbi The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site
    Maria C Bewley
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 42:13145-51. 2003
  3. ncbi Expression of a novel P275L variant of NADH:cytochrome b5 reductase gives functional insight into the conserved motif important for pyridine nucleotide binding
    M J Percy
    Department of Hematology, Floor C, Tower Block, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, Northern Ireland, UK
    Arch Biochem Biophys 447:59-67. 2006
  4. ncbi Heterogeneity of the rat NADH-cytochrome-b5-reductase transcripts resulting from multiple alternative first exons
    L Mota Vieira
    Institute Cochin de Genetique Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 129, Paris, France
    Eur J Biochem 220:729-37. 1994
  5. ncbi Molecular basis of recessive congenital methemoglobinemia, types I and II: Exon skipping and three novel missense mutations in the NADH-cytochrome b5 reductase (diaphorase 1) gene
    W Kugler
    Universitäts Kinderklinik, Robert Koch Str 40, D 37075 Gottingen, Germany
    Hum Mutat 17:348. 2001
  6. ncbi Concise review: methemoglobinemia
    A Mansouri
    Department of Medicine, Overton Brooks VAMC, Shreveport, Louisiana 71101 4295
    Am J Hematol 42:7-12. 1993
  7. ncbi A compound heterozygote in the NADH-cytochrome b5 reductase gene from a Chinese patient with hereditary methemoglobinemia type I
    Y S Wu
    Center for Medical Laboratory, Fuzhou General Hospital, People s Republic of China
    Int J Hematol 72:34-6. 2000
  8. doi A novel L218P mutation in NADH-cytochrome b5 reductase associated with type I recessive congenital methemoglobinemia
    Tugba Arikoglu
    Department of Pediatric Hematology, Dr Sami Ulus Children s Health Education and Research Hospital, Ankara, Turkey
    Pediatr Hematol Oncol 26:381-5. 2009
  9. ncbi Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase
    Glenn W Roma
    Department of Biochemistry and Molecular Biology, College of Medicine and the H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
    Biochemistry 44:13467-76. 2005
  10. ncbi Biosynthesis of N-glycolylneuraminic acid-containing glycoconjugates. Purification and characterization of the key enzyme of the cytidine monophospho-N-acetylneuraminic acid hydroxylation system
    T Kawano
    Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Japan
    J Biol Chem 269:9024-9. 1994

Research Grants

Detail Information

Publications240 found, 100 shown here

  1. ncbi Soluble cytochrome b 5 reductase from human erythrocytes
    P G Passon
    Biochim Biophys Acta 275:62-73. 1972
  2. ncbi The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site
    Maria C Bewley
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 42:13145-51. 2003
    ....
  3. ncbi Expression of a novel P275L variant of NADH:cytochrome b5 reductase gives functional insight into the conserved motif important for pyridine nucleotide binding
    M J Percy
    Department of Hematology, Floor C, Tower Block, Belfast City Hospital, Lisburn Road, Belfast BT9 7AB, Northern Ireland, UK
    Arch Biochem Biophys 447:59-67. 2006
    ..of recessive congenital methemoglobinemia (RCM, OMIN 250800) is associated with mutations in NADH:cytochrome b5 reductase (cb5r) and manifests as cyanosis from birth...
  4. ncbi Heterogeneity of the rat NADH-cytochrome-b5-reductase transcripts resulting from multiple alternative first exons
    L Mota Vieira
    Institute Cochin de Genetique Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 129, Paris, France
    Eur J Biochem 220:729-37. 1994
    ..the mechanisms responsible for the generation of different isoforms (membrane-bound and soluble) of NADH-cytochrome b5 reductase, and the different clinical forms of recessive congenital methemoglobinemia due to the deficiency of this ..
  5. ncbi Molecular basis of recessive congenital methemoglobinemia, types I and II: Exon skipping and three novel missense mutations in the NADH-cytochrome b5 reductase (diaphorase 1) gene
    W Kugler
    Universitäts Kinderklinik, Robert Koch Str 40, D 37075 Gottingen, Germany
    Hum Mutat 17:348. 2001
    Hereditary methemoglobinemia due to reduced nicotin amide adenine dinucleotide (NADH)-cytochrome b5 reductase (b5r) deficiency is classified into an erythrocyte type (I) and a generalized type (II)...
  6. ncbi Concise review: methemoglobinemia
    A Mansouri
    Department of Medicine, Overton Brooks VAMC, Shreveport, Louisiana 71101 4295
    Am J Hematol 42:7-12. 1993
    ..Under physiological conditions, methemoglobin reduction is accomplished mainly by red cell NADH-cytochrome b5 reductase (NADH-methemoglobin reductase) so efficiently that there is insignificant amounts of methemoglobin in the ..
  7. ncbi A compound heterozygote in the NADH-cytochrome b5 reductase gene from a Chinese patient with hereditary methemoglobinemia type I
    Y S Wu
    Center for Medical Laboratory, Fuzhou General Hospital, People s Republic of China
    Int J Hematol 72:34-6. 2000
    To elucidate the mutation in the nicotinamide adenine dinucleotide-cytochrome b5 reductase (b5R) gene from a Chinese patient with hereditary methemoglobinemia type I, we analyzed the coding sequences of b5R cDNA from the patient and from ..
  8. doi A novel L218P mutation in NADH-cytochrome b5 reductase associated with type I recessive congenital methemoglobinemia
    Tugba Arikoglu
    Department of Pediatric Hematology, Dr Sami Ulus Children s Health Education and Research Hospital, Ankara, Turkey
    Pediatr Hematol Oncol 26:381-5. 2009
    ..Congenital methemoglobinemia due to deficiency of nicotinamide-adenine dinucleotide (NADH)-cytochrome b5 reductase (cb(5)r) is an autosomal recessive disorder characterized by life long cyanosis...
  9. ncbi Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase
    Glenn W Roma
    Department of Biochemistry and Molecular Biology, College of Medicine and the H Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida 33612, USA
    Biochemistry 44:13467-76. 2005
    b>Cytochrome b5 reductase (cb5r), a member of the ferredoxin:NADP+ reductase family of flavoprotein transhydrogenases, catalyzes the NADH-dependent reduction of cytochrome b5...
  10. ncbi Biosynthesis of N-glycolylneuraminic acid-containing glycoconjugates. Purification and characterization of the key enzyme of the cytidine monophospho-N-acetylneuraminic acid hydroxylation system
    T Kawano
    Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Japan
    J Biol Chem 269:9024-9. 1994
    ..experiment with the purified CMP-NeuAc hydroxylase, soluble cytochrome b5, and recombinant NADH-cytochrome b5 reductase revealed that these three factors are essential for the reaction...
  11. ncbi Rate of alteration of hepatic mixed-function oxidase system in rats fed different dietary fats
    A Ammouche
    Institut National de la Sante et de la Recherche Medicale, Unité 26, Hopital Fernand Widal, France
    Biochem Cell Biol 71:530-7. 1993
    ..These enzymes were correlated very well (0.84 < r < 0.93), P < 0.05 with dietary sigma polyunsaturated fatty acids (n-3).(ABSTRACT TRUNCATED AT 250 WORDS)..
  12. ncbi Methemoglobinemia and eccentrocytosis in equine erythrocyte flavin adenine dinucleotide deficiency
    J W Harvey
    College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA
    Vet Pathol 40:632-42. 2003
    ..This deficiency in FAD results from a defect in erythrocyte riboflavin metabolism, which has not been documented previously in animals...
  13. ncbi [Congenital methemoglobinemia: a rare case of cyanosis in the newborn]
    L Schwartz
    Laboratoire d hématologie EFS Lorraine Champagne, Vandoeuvre les Nancy
    Ann Biol Clin (Paris) 63:314-6. 2005
    ..It is due to the recessive autosomal NADH-cytochrome b5 reductase (EC. 1.6.2.2) deficiency...
  14. ncbi Functional analysis of vaccinia virus B5R protein: role of the cytoplasmic tail
    M M Lorenzo
    , Madrid, Valdeolmos, 28130, Spain
    Virology 252:450-7. 1998
    ..The B5R gene encodes an EEV-specific type I membrane protein, and deletion of this gene markedly decreases EEV formation ..
  15. ncbi Superoxide, neuroleptics and the ubiquinone and cytochrome b5 reductases in brain and lymphocytes from normals and schizophrenic patients
    S A Whatley
    Department of Neuroscience, Institute of Psychiatry, Denmark Hill, London, UK
    Mol Psychiatry 3:227-37. 1998
    ..The neuroleptic flupenthixol reduces the expression in rats of the gene coding for NADH-cytochrome b5 reductase as measured by in situ hybridisation and its enzymic manifestation...
  16. pmc A linkage and physical map of chromosome 22, and some applications to gene mapping
    C Julier
    , , Paris, France
    Am J Hum Genet 42:297-308. 1988
    ..Analysis of families segregating recessive congenital methemoglobinemia (RCM), a disease in which the cytochrome b5 reductase is defective, as well as of families with cases of hereditary low levels of cytochrome b5 reductase ..
  17. ncbi Stimulatory effects of lung cytochrome b5 on benzphetamine N-demethylation in a reconstituted system containing lung cytochrome P450LgM2
    E Arinc
    Department of Biology, Middle East Technical University, Ankara, Turkey
    Int J Biochem 26:1033-42. 1994
    ..purified cytochrome b5 fractions were free of cytochrome P450, NADPH-cytochrome P450 reductase and NADH-cytochrome b5 reductase activities...
  18. ncbi A selective decrease in the xenobiotic metabolizing activity of rat lungs by nitrogen dioxide exposures
    Y Takahashi
    Basic Medical Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan
    Toxicology 54:281-96. 1989
    ..On the other hand, the cytochrome b5, NADPH-cytochrome P-450 reductase and NADH-cytochrome b5 reductase of lung microsomes were increased concomitant with increase in microsomal proteins during 2-week ..
  19. ncbi Microarray assay for evaluation of the genetic stability of modified vaccinia virus Ankara B5R gene
    Majid Laassri
    Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, Maryland 20852, USA
    J Med Virol 79:791-802. 2007
    ..vaccine, an advanced oligonucleotide microchip was developed and used to assay for mutations that could emerge in B5R gene, a vaccinia virus gene encoding for a protein that contains very important neutralizing epitopes...
  20. ncbi Abnormalities in mitochondria-associated membranes and phospholipid biosynthetic enzymes in the mnd/mnd mouse model of neuronal ceroid lipofuscinosis
    J E Vance
    Lipid and Lipoprotein Research Group, University of Alberta, Edmonton, Canada
    Biochim Biophys Acta 1344:286-99. 1997
    ..However, 3 proteins (cytochrome b5, NADH:cytochrome b5 reductase and mitochondrial F1Fzero-ATP synthase c subunit) which are normally present in mitochondria were ..
  21. ncbi Cytochrome b5 reductase: role of the si-face residues, proline 92 and tyrosine 93, in structure and catalysis
    Christopher C Marohnic
    Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa, Florida 33612, USA
    Biochemistry 44:2449-61. 2005
    ....
  22. doi Chemopreventive and therapeutic modulation of green tea polyphenols on drug metabolizing enzymes in 4-Nitroquinoline 1-oxide induced oral cancer
    Periasamy Srinivasan
    Team for Radiation Food Science and Biotechnology, Radiation Application Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk 580 185, Republic of Korea
    Chem Biol Interact 172:224-34. 2008
    ..the effect of green tea polyphenols (GTP) on the activities of cytochrome b5, cytochrome P450, cytochrome b5 reductase (cyt b5 R), cytochrome P450 reductase (cyt P450 R), arryl hydrocarbon hydroxylase (AHH), DT-diaphorase (..
  23. pmc Methemoglobinemia in a young man
    Yasmin S Hamirani
    Department of Adult Cardiology, Texas Heart Institute at St Luke s Episcopal Hospital, Baylor College of Medicine Houston, Texas 77030 2304, USA
    Tex Heart Inst J 35:76-7. 2008
    ..Later, a DNA analysis, reported separately by others, showed that the patient's methemoglobinemia was caused by a novel mutation of the cytochrome b5 reductase gene.
  24. pmc DT-diaphorase activity in normal and neoplastic human tissues; an indicator for sensitivity to bioreductive agents?
    E Smitskamp-Wilms
    Department of Oncology, Free University Hospital, Amsterdam, The Netherlands
    Br J Cancer 72:917-21. 1995
    ..extracts using 2,6-dichlorophenolindophenol (DCPIP) and NADH as substrates; cytochrome P450 reductase or cytochrome b5 reductase activities were assessed with cytochrome c and NADPH or NADH respectively...
  25. ncbi Determination of lumenal orientation of microsomal 50-kDa esterase/N-deacetylase
    J Ozols
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
    Biochemistry 37:10336-44. 1998
    ..at the conditions which led to the removal of the membranous segments from cytochrome b5 and the NADH-cytochrome b5 reductase. The proposed model of the 50-kDa protein predicts one transmembrane segment at the N-terminus, flanked ..
  26. pmc Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox
    J W Hooper
    Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702, USA
    J Virol 78:4433-43. 2004
    ..that rhesus macaques vaccinated with a DNA vaccine consisting of four vaccinia virus genes (L1R, A27L, A33R, and B5R) were protected from severe disease after an otherwise lethal challenge with monkeypox virus...
  27. pmc Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 A resolution
    Sangwoo Kim
    Institute for Protein Research, Osaka University, 3 2 Yamada Oka, Suita, Osaka, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 63:274-9. 2007
    ..The differences in these structural properties between the two proteins were consistent with estimates of their thermostabilities obtained from differential scanning calorimetry data...
  28. ncbi Role of Thr(66) in porcine NADH-cytochrome b5 reductase in catalysis and control of the rate-limiting step in electron transfer
    Shigenobu Kimura
    Department of Life Science, Graduate School of Science, Himeji Institute of Technology, Kouto 3 2 1, Kamigori, Hyogo 678 1297, Japan
    J Biol Chem 278:3580-9. 2003
    ..On the basis of these data, we present a new kinetic scheme to explain the mechanism of electron transfer from NADH to one-electron acceptors including cytochrome b(5)...
  29. pmc Differential antigen requirements for protection against systemic and intranasal vaccinia virus challenges in mice
    David R Kaufman
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Virol 82:6829-37. 2008
    ..intracellular mature virion (IMV) antigens A27L and L1R and extracellular enveloped virion (EEV) antigens A33R and B5R in a murine vaccinia virus challenge model...
  30. doi Investigation of orf virus structure and morphogenesis using recombinants expressing FLAG-tagged envelope structural proteins: evidence for wrapped virus particles and egress from infected cells
    Joanne L Tan
    Virus Research Unit, Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin, New Zealand
    J Gen Virol 90:614-25. 2009
    ..Notable exceptions are A36R, K2L, A56R and B5R, which are associated with WV and EV envelopes...
  31. ncbi Biochemical evidence that high concentrations of the antidepressant amoxapine may cause inhibition of mitochondrial electron transport
    A M Roberton
    Department of Biochemistry, University of Auckland, New Zealand
    Toxicol Appl Pharmacol 93:118-26. 1988
    ..Microsomal NADH-cytochrome b5 reductase was inhibited by amoxapine, but at higher amoxapine concentrations than mitochondrial reactions...
  32. ncbi Evaluation of the methemoglobinemia associated with sulofenur
    D C Molthrop
    Department of Medicine, Cooper Green Hospital, Birmingham, AL
    Invest New Drugs 12:99-102. 1994
    ..We studied the incidence of methemoglobinemia, sulfhemoglobinemia and cytochrome b5 reductase deficiency in nine consecutive patients enrolled in a phase II trials using Sulofenur...
  33. ncbi The vaccinia virus 42-kDa envelope protein is required for the envelopment and egress of extracellular virus and for virus virulence
    M Engelstad
    Sir William Dunn School of Pathology, University of Oxford, England
    Virology 194:627-37. 1993
    Vaccinia virus gene B5R encodes a M(r) 42K glycoprotein that is expressed throughout infection and forms part of the envelope of extracellular virus...
  34. ncbi Electrostatic interaction between NADH-cytochrome b5 reductase and cytochrome b5 studied by site-directed mutagenesis
    K Shirabe
    Department of Biochemistry, Oita Medical University, Japan
    Biochim Biophys Acta 1384:16-22. 1998
    Electrostatic interaction between NADH-cytochrome b5 reductase and cytochrome b5 was studied by site-directed mutagenesis...
  35. ncbi Rat oesophageal cytochrome P450 (CYP) monooxygenase system: comparison to the liver and relevance in N-nitrosodiethylamine carcinogenesis
    L F Pinto
    Departamento de Bioquimica, IBRAG, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro, 87, fundos, 4 degrees andar, Vila Isabel, Rio de Janeiro, RJ, Brasil
    Carcinogenesis 22:1877-83. 2001
    ..Total amount of cytochrome P450, NADPH P450 reductase, cytochrome b5 and cytochrome b5 reductase of the oesophageal mucosa was approximately 7% of what was found in the liver...
  36. ncbi Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase
    Benjamin J Curry
    ARC Centre of Excellence for Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    Genomics 83:425-38. 2004
    Cytochrome b5/cytochrome b5 reductase (cb5/cb5r) is a cytosolic fusion protein between the hemoprotein cytochrome b5 and the flavoprotein cytochrome b5 reductase...
  37. ncbi Redundancy or flexibility: molecular diversity of the electron transfer components for P450 monooxygenases in higher plants
    Daisaku Ohta
    Graduate School of Agriculture and Biosciences, Osaka Prefecture University, 1 1 Gakuen cho, Sakai 599 8531, Japan
    Front Biosci 9:1587-97. 2004
    ..hand, molecular diversity has also been reported for NADPH-cytochrome P450 reductase, cytochrome b5, and cytochrome b5 reductase in plants...
  38. ncbi Recessive congenital methaemoglobinaemia: functional characterization of the novel D239G mutation in the NADH-binding lobe of cytochrome b5 reductase
    M J Percy
    Department of Haematology, Belfast City Hospital, Belfast, UK
    Br J Haematol 129:847-53. 2005
    ..Thus cytb(5)r deficient patients who are heterozygous for an NADH-binding lobe mutation can exhibit the clinically less severe type I phenotype, even in association with heterozygous deletion of the NADH-binding lobe...
  39. pmc LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox
    Masayuki Saijo
    Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama, Tokyo 208 0011, Japan
    J Virol 80:5179-88. 2006
    ..attenuated vaccinia virus vaccine strain LC16m8, with a mutation in the important immunogenic membrane protein B5R, to induce protective immunity against monkeypox in nonhuman primates was evaluated in comparison with the parental ..
  40. ncbi Analysis of tetramethylrhodamine-labeled actin polymerization and interaction with actin regulatory proteins
    Andrea Pelikan Conchaudron
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurale, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 281:24036-47. 2006
    ..has also been used to solve the structure of actin in complex with the formin homology 2 domain of mammalian Dia1. To understand how the covalent modification of actin by TMR may affect the structural changes linked to ATP ..
  41. pmc Reductive detoxification of arylhydroxylamine carcinogens by human NADH cytochrome b5 reductase and cytochrome b5
    Joseph R Kurian
    Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706 1102, USA
    Chem Res Toxicol 19:1366-73. 2006
    ..we hypothesized that the reductive detoxification of arylhydroxylamine carcinogens was catalyzed by NADH cytochrome b5 reductase (b5R) and cytochrome b5 (cyt b5)...
  42. pmc Mitf regulation of Dia1 controls melanoma proliferation and invasiveness
    Suzanne Carreira
    Signalling and Development Laboratory, Marie Curie Research Institute, Oxted, Surrey, RH8 0TL, United Kingdom
    Genes Dev 20:3426-39. 2006
    ..transcription factor, Mitf, via regulation of the DIAPH1 gene encoding the diaphanous-related formin Dia1 that promotes actin polymerization and coordinates the actin cytoskeleton and microtubule networks at the cell ..
  43. pmc Identifying autism loci and genes by tracing recent shared ancestry
    Eric M Morrow
    Division of Genetics, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Science 321:218-23. 2008
    ..The largest deletions implicated genes, including PCDH10 (protocadherin 10) and DIA1 (deleted in autism1, or c3orf58), whose level of expression changes in response to neuronal activity, a marker of ..
  44. doi A novel mutation in type II methemoglobinemia
    Michelle P Hudspeth
    Department of Pediatrics, Division of Pediatric Hematology Oncology, Medical University of South Carolina, Charleston, South Carolina, USA
    J Child Neurol 25:91-3. 2010
    Type II methemoglobinemia is a somatic deficiency of cytochrome b5 reductase with severe global neurologic impairment...
  45. pmc Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates
    Jay W Hooper
    US Army Medical Research Institute of Infectious Diseases, Virology Division, 1425 Porter Street, Fort Detrick, MD 21702, United States
    Vaccine 28:494-511. 2009
    ..Here, we demonstrated that virus-like replicon particles (VRPs) expressing the vaccinia virus A33R, B5R, A27L, and L1R genes elicited protective immunity in mice comparable to vaccination with live-vaccinia virus...
  46. pmc CYB5R3: a key player in aerobic metabolism and aging?
    Rafael De Cabo
    Laboratory of Experimental Gerontology, National Institute on Aging, NIH, Baltimore, MD 21224, USA
    Aging (Albany NY) 2:63-8. 2010
    ..Here we review the activities of a redox enzyme (NQR1 in yeast and CYB5R3 in mammals) that also influences the NAD(+)/NADH ratio and may play a regulatory role that connects aerobic metabolism with aging...
  47. ncbi Cetacaine induced methemoglobinemia: overview of analysis and treatment strategies
    Rizwan Khan
    Camden Clark Medical Center, Department of Internal Medicine, Parkersburg, WV, USA
    W V Med J 109:24-6. 2013
    ..Inherited causes include an enzymatic deficiency in the enzyme cytochrome b5 reductase where as acquired causes are most commonly from routinely used medications...
  48. doi Modulatory effect of troxerutin on biotransforming enzymes and preneoplasic lesions induced by 1,2-dimethylhydrazine in rat colon carcinogenesis
    Rajamanickam Vinothkumar
    Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India
    Exp Mol Pathol 96:15-26. 2014
    ..such as cytochrome P450, cytochrome b5, cytochrome P4502E1, NADPH-cytochrome P450 reductase and NADH-cytochrome b5 reductase and reduced activities of phase II enzymes such as glutathione-S-transferase (GST), DT-diaphorase (DTD) ..
  49. pmc Identification of 10 cowpox virus proteins that are necessary for induction of hemorrhagic lesions (red pocks) on chorioallantoic membranes
    Zhiyong Xu
    Institut fur Virologie, Freie Universitat Berlin, Zentrum für Infektionsmedizin, Berlin, Germany
    J Virol 88:8615-28. 2014
    ..The genes are the homologues of F12L, F15L, E2L, E3L, E8R, A4L, A33R, A34R, A36R, and B5R of vaccinia virus (VACV)...
  50. doi A 5-hour-old male neonate with cyanosis
    Melkon Dombourian
    Departments of Clinical Pathology and
    Lab Med 46:60-3; quiz e14. 2015
    ..The patient was subsequently diagnosed with a congenital cytochrome b5 reductase deficiency...
  51. pmc The pivotal role of the mitochondrial amidoxime reducing component 2 in protecting human cells against apoptotic effects of the base analog N6-hydroxylaminopurine
    Birte Plitzko
    From the Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical Institute, Christian Albrechts University of Kiel, 24118 Kiel, Germany
    J Biol Chem 290:10126-35. 2015
    ..and nucleosides upon reconstitution with the electron transport proteins cytochrome b5 and NADH-cytochrome b5 reductase. By RNAi-mediated down-regulation of mARC in human cell lines the mARC-dependent N-reductive detoxication ..
  52. doi Methemoglobin reduction by NADH-cytochrome b(5) reductase in Propsilocerus akamusi larvae
    Shintaro Maeda
    Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Tokyo 101 8308, Japan
    Comp Biochem Physiol B Biochem Mol Biol 185:54-61. 2015
    ..NADH-dependent reduction of metHb in coelomic fluid suggested the coexistence of cytochrome b5 reductase (b5R) and cytochrome b5 with hemoglobin in the fluid and that these proteins were involved in ..
  53. pmc NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer
    Rikke R Lund
    From the Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, J B Winsløws Vej 25 3, DK 5000 Odense C, Denmark
    Mol Cell Proteomics 14:2988-99. 2015
    ..altered expression in isogenic metastatic versus nonmetastatic cancer cell lines, including NADH-cytochrome b5 reductase 3 (CYB5R3), l-lactate dehydrogenase A (LDHA), Niemann-pick c1 protein (NPC1), and nucleolar RNA helicase ..
  54. doi MicroRNA library screening identifies growth-suppressive microRNAs that regulate genes involved in cell cycle progression and apoptosis
    Young Chul Choi
    Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea
    Exp Cell Res 339:320-32. 2015
    ..In miR-608-transfected cells, B cell lymphoma 2-like 1 (BCL2L1), D-type cyclin 1 (CCND1), CCND3, cytochrome b5 reductase 3 (CYB5R3), phosphoinositide 3-kinase regulatory subunit 2 (PIK3R2), specificity protein 1 (SP1), and ..
  55. pmc Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss
    Takehiko Ueyama
    Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Japan
    EMBO Mol Med 8:1310-1324. 2016
    DIAPH1 encodes human DIA1, a formin protein that elongates unbranched actin. The c...
  56. ncbi High-level expression in Escherichia coli of the catalytically active flavin domain of corn leaf NADH:nitrate reductase and its comparison to human NADH:cytochrome B5 reductase
    G E Hyde
    Plant Biotechnology Group, Michigan Technological University, Houghton 49931
    Biochem Biophys Res Commun 168:1285-91. 1990
    ..The recombinant flavin domain has a ferricyanide reductase specific activity of 1000 mumols NADH oxidized/min/mg protein and a visible spectrum virtually identical to that of human NADH:cytochrome b5 reductase.
  57. ncbi Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene
    S Zenno
    Research Laboratory for Genetic Information, Kyushu University, Fukuoka
    J Biochem 107:810-6. 1990
    Rat cDNA encoding NADH-cytochrome b5 reductase (b5R) was isolated from a rat liver cDNA library using a human b5R cDNA as a probe...
  58. ncbi [Effect of the cholesterol and protein diet on cytochrome P-450 dependent monooxygenase activity]
    A Plewka
    Katedry Histologii i Embriologii Slaskiej Akademii Medycznej w Katowicach
    Med Pr 46:17-23. 1995
    ..Cytochrome P-450 and cytochrome b5 contents, NADPH-cytochrome P-450 reductase, NADH-cytochrome b5 reductase, aniline hydroxylase and 4-aminopyrine N-demethylase activities were measured in a hepatic microsomal ..
  59. ncbi Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH
    H Maeda
    Department of Microbiology, Kumamoto University School of Medicine, Japan
    Cancer Lett 143:117-21. 1999
    ..Here we present more evidence in which O2*- is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro...
  60. pmc Transcriptional and translational mechanisms of cytochrome b5 reductase isoenzyme generation in humans
    A Leroux
    Institut Cochin de Genetique Moleculaire, U 129 INSERM Unité de Recherches en Physiologie et Pathologie Génétiques et Moléculaires, Universite Rene Descartes, 24 rue du Faubourg Saint Jacques, 75014 Paris, France
    Biochem J 355:529-35. 2001
    b>Cytochrome b5 reductase (b5R) is an essential enzyme that exists in soluble and membrane-bound isoforms, each with specific functions. In the rat, the two forms are generated from alternative transcripts differing in the first exons...
  61. pmc Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13L protein and phospholipase D
    Matloob Husain
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 76:7777-89. 2002
    ..Two viral proteins, encoded by the F13L and B5R open reading frames, are essential for the membrane-wrapping step...
  62. ncbi Reduction of sulfamethoxazole and dapsone hydroxylamines by a microsomal enzyme system purified from pig liver and pig and human liver microsomes
    Bernd Clement
    Pharmazeutisches Institut, Christian Albrechts Universitat, Gutenbergstr 76, D 24118 Kiel, Germany
    Life Sci 77:205-19. 2005
    ..of benzamidoxime an oxygen-insensitive liver microsomal enzyme system that required cytochrome b5, NADH-cytochrome b5 reductase and a cytochrome P450 isoenzyme of the subfamily 2D has been described...
  63. ncbi Expression and characterization of a functional canine variant of cytochrome b5 reductase
    Glenn W Roma
    Department of Molecular Medicine, School of Basic Biomedical Sciences, College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, MDC 007, Tampa, FL 33612, USA
    Arch Biochem Biophys 452:69-82. 2006
    b>Cytochrome b5 reductase (cb5r), a member of the flavoprotein transhydrogenase family of oxidoreductase enzymes, catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5...
  64. pmc Characterization and use of mammalian-expressed vaccinia virus extracellular membrane proteins for quantification of the humoral immune response to smallpox vaccines
    Alonzo D Garcia
    Laboratory of DNA Viruses, Division of Viral Products, Center for Biologics and Evaluation and Research FDA, 1401 Rockville Pike, HFM 457, Rockville, MD 20892, USA
    Clin Vaccine Immunol 14:1032-44. 2007
    ..Quantitatively similar titers of antibodies against EV antigens A33R, A56R, and B5R were measured in mice vaccinated with Dryvax and MVA when MVA was administered at a dose of 10(8) plaque-forming ..
  65. doi Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli
    Neda Setayesh
    Laboratory of Genetic Engineering, Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14174, Iran
    Biol Res 42:137-46. 2009
    The present work aims to study a new NADH-cytochrome b5 reductase (cb5r) from Mucor racemosus PTCC 5305. A cDNA coding for cb5r was isolated from a Mucor racemosus PTCC 5305 cDNA library...
  66. pmc Individual variability in the detoxification of carcinogenic arylhydroxylamines in human breast
    Keelia Rhoads
    Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706 1102, USA
    Toxicol Sci 121:245-56. 2011
    Cytochrome b(5) (b5) and NADH cytochrome b(5) reductase (b5R) detoxify reactive hydroxylamine (NHOH) metabolites of known arylamine and heterocyclic amine mammary carcinogens...
  67. ncbi Activation and detoxification metabolism of urban air pollutants 2-nitrobenzanthrone and carcinogenic 3-nitrobenzanthrone by rat and mouse hepatic microsomes
    Marie Stiborova
    Department of Biochemistry, Charles University, Prague, Czech Republic
    Neuro Endocrinol Lett 33:8-15. 2012
    ..hepatic microsomes containing cytochromes P450 (CYPs), their reductase (NADPH:CYP reductase), and NADH:cytochrome b5 reductase was investigated under anaerobic and aerobic conditions...
  68. doi Evidence that cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the hepatic cytochrome P450 system
    Colin J Henderson
    Division of Cancer Research, Medical Research Institute, University of Dundee, Jacqui Wood Cancer Centre, Ninewells Hospitaland Medical School, Dundee, United Kingdom
    Mol Pharmacol 83:1209-17. 2013
    ..These data provide strong evidence that cytochrome b(5)/cytochrome b(5) reductase can act as a sole electron donor to the P450 system in vitro and in vivo...
  69. doi In vitro effects of myricetin, morin, apigenin, (+)-taxifolin, (+)-catechin, (-)-epicatechin, naringenin and naringin on cytochrome b5 reduction by purified NADH-cytochrome b5 reductase
    Haydar Celik
    Department of Pharmaceutical Basic Sciences, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey
    Toxicology 308:34-40. 2013
    The microsomal NADH-dependent electron transport system consisting of cytochrome b5 reductase and cytochrome b5 participates in a number of physiologically important processes including lipid metabolism as well as is involved in the ..
  70. pmc Suppression of NYVAC Infection in HeLa Cells Requires RNase L but Is Independent of Protein Kinase R Activity
    Mercedes Fernández-Escobar
    Department of Preventive Medicine, Public Health and Microbiology, Universidad Autonoma, Madrid, Spain
    J Virol 90:2135-41. 2015
    ..abortive infection of human HeLa cells with the poxvirus strain NYVAC, for which an inhibition of viral A27L and B5R gene expression is described...
  71. ncbi Studies on the microsomal electron-transport system of anaerobically grown yeast. IV. Purification and characterization of NADH-cytochrome b5 reductase
    S Kubota
    J Biochem 81:187-95. 1977
    The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification...
  72. ncbi The sequence of squash NADH:nitrate reductase and its relationship to the sequences of other flavoprotein oxidoreductases. A family of flavoprotein pyridine nucleotide cytochrome reductases
    G E Hyde
    Phytotechnology Research Center, Michigan Technological University, Houghton 49931
    J Biol Chem 266:23542-7. 1991
    ..has significant similarity to the amino acid sequences of sulfite oxidase, cytochrome b5, and NADH:cytochrome b5 reductase. Alignment of these sequences with that of squash defines domains of nitrate reductase that appear to ..
  73. ncbi Bacterial synthesis of active rat stearyl-CoA desaturase lacking the 26-residue amino-terminal amino acid sequence
    P Strittmatter
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06032
    J Biol Chem 263:2532-5. 1988
    ..during induction, results in the formation of active holoenzyme which can be reconstituted with NADH cytochrome b5 reductase and cytochrome b5 to form an active stearyl-CoA desaturase system...
  74. pmc Immunocytochemistry of calciosomes in liver and pancreas
    S Hashimoto
    Consiglio Nazionale delle Ricerche Center of Cytopharmacology, University of Milano, Italy
    J Cell Biol 107:2523-31. 1988
    ..demonstrated that hepatic calciosomes express neither two ER markers (cytochrome-P450 and NADH-cytochrome b5 reductase) nor the endolysosome marker, luminal acidity (revealed by 3-[2,4-dinitroanilino]-3'-amino-N-methyl ..
  75. ncbi Identification of an "essential" cysteine of nitrate reductase via mutagenesis of its recombinant cytochrome b reductase domain
    U N Dwivedi
    Phytotechnology Research Center, Michigan Technological University, Houghton 49931 1295
    J Biol Chem 269:13785-91. 1994
    ..near the C terminus, which is found in all nitrate reductases and also in the closely related NADH: cytochrome b5 reductase, as well as, most other members of this flavoenzyme family...
  76. pmc Deletion of the vaccinia virus B5R gene encoding a 42-kilodalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination
    E J Wolffe
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    J Virol 67:4732-41. 1993
    ..role of gp42, a glycoprotein component of the extracellular enveloped form of vaccinia virus (EEV) encoded by the B5R gene...
  77. ncbi Targeting proteins to the lumen of endoplasmic reticulum using N-terminal domains of 11beta-hydroxysteroid dehydrogenase and the 50-kDa esterase
    H Mziaut
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Biol Chem 274:14122-9. 1999
    ..general applicability of the proposed LTS, we fused the N terminus of E3 to the N terminus of the NADH-cytochrome b5 reductase lacking the myristoyl group and N-terminal 30-residue membrane anchor...
  78. pmc Adaptation of cytochrome-b5 reductase activity and methaemoglobinaemia in areas with a high nitrate concentration in drinking-water
    S K Gupta
    Satellite Hospital, Banipark, Jaipur, India
    Bull World Health Organ 77:749-53. 1999
    ....
  79. ncbi Usefulness of statistic experimental designs in enzymology: example with recombinant hCYP3A4 and 1A2
    B Bournique
    Rhone Poulenc Rorer, Drug Metabolism and Pharmacokinetics, and Pharmaceutical Sciences, 13 Quai Jules Guesdes, Vitry s Seine Cedex, 94403, France
    Anal Biochem 276:18-26. 1999
    ..Later experiments confirmed the predictivity of the models and the efficiency of optimized conditions. This approach can be applied to other biochemistry areas...
  80. ncbi A novel plasma membrane quinone reductase and NAD(P)H:quinone oxidoreductase 1 are upregulated by serum withdrawal in human promyelocytic HL-60 cells
    Nathalie Forthoffer
    Departamento de Biologia Celular, Fisiología e Immunología, Facultad de Ciencias, Universidad de Cordoba, Spain
    J Bioenerg Biomembr 34:209-19. 2002
    ..An enzyme with properties of a cytochrome b5 reductase accounted for most dicumarol-resistant quinone reductase activity in HL-60 plasma membranes...
  81. ncbi Nuclear overexpression of NADH:cytochrome b5 reductase activity increases the cytotoxicity of mitomycin C (MC) and the total number of MC-DNA adducts in Chinese hamster ovary cells
    Kathleen M Holtz
    Department of Pharmacology, Yale Cancer Center, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 278:5029-34. 2003
    ..The results indicate that the cytotoxicity of MC is modulated by the subcellular location of FpD, with greater cell kill occurring when bioactivation occurs in the proximity of its target, nuclear DNA...
  82. ncbi Cytochrome b5 reductase and cytochrome b5 support the CYP2E1-mediated activation of nitrosamines in a recombinant Ames test
    Vishwesh Mokashi
    Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536 0305, USA
    Arch Biochem Biophys 412:147-52. 2003
    ..Neither cytochrome b(5) reductase nor cytochrome b(5) alone could support CYP2E1 activity. These results demonstrate that the cytochrome b(5) reductase/cytochrome b(5) pathway can support CYP2E1 activity in bacterial cells...
  83. ncbi Severe neurological impairment in hereditary methaemoglobinaemia type 2
    Sandra P Toelle
    Division of Neurology, University Children s Hospital, Steinwiesstrasse 75, 8032 Zurich, Switzerland
    Eur J Pediatr 163:207-9. 2004
    Recessive congenital methaemoglobinaemia (RCM) due to NADH-cytochrome b5 reductase (cytb5r) deficiency is a very rare disorder. We report on two unrelated patients (4 and 2.5 years old) with RCM type 2...
  84. ncbi Improved protection conferred by vaccination with a recombinant vaccinia virus that incorporates a foreign antigen into the extracellular enveloped virion
    Heesun Kwak
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6073, USA
    Virology 322:337-48. 2004
    ..We therefore constructed a recombinant vaccinia virus that replaced the cytoplasmic domain of the B5R protein with a test antigen, HIV-1 Gag...
  85. ncbi Two-cistronic expression plasmids for high-level gene expression in Escherichia coli preventing translational initiation inhibition caused by the intramolecular local secondary structure of mRNA
    Shigenobu Kimura
    Graduate School of Life Science, University of Hyogo, 3 2 1 Kouto, Kamigori, Hyogo 678 1297
    J Biochem 137:523-33. 2005
    ..Using these plasmids, the solubilized donain of porcine NADH-cytochrome b5 reductase was also highly accumulated on prevention of the translational initiation inhibition...
  86. ncbi Assembly and mechanosensory function of focal adhesions: experiments and models
    Alexander D Bershadsky
    Department of Molecular Cell Biology, The Weizmann Institute of Science, P O Box 26, Rehovot 76100, Israel
    Eur J Cell Biol 85:165-73. 2006
    ..G-protein Rho via activation of two major targets, Rho-associated kinase (ROCK) and the formin homology protein, Dia1. ROCK controls creation of myosin II-driven forces, while Dia1 is involved in the response of FAs to these forces...
  87. ncbi Methemoglobinemia caused by the ingestion of courgette soup given in order to resolve constipation in two formula-fed infants
    Francesco Savino
    Dipartimento di Scienze Pediatriche e dell Adolescenza, Universita di Torino, Azienda Ospedaliera OIRM S Anna, Torino, Italy
    Ann Nutr Metab 50:368-71. 2006
    ..lt; 3 months of age are more susceptible than adults because of lower amounts of a key enzyme, NADH-cytochrome b5 reductase, which converts methemoglobin back to hemoglobin...
  88. pmc cis-Acting site controlling bidirectional transcription at the growth-differentiation transition in Dictyostelium discoideum
    Shigenori Hirose
    Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0368, USA
    Eukaryot Cell 5:1104-10. 2006
    A pair of adjacent genes, impA and dia1, are divergently transcribed but expressed at different stages in the life cycle of Dictyostelium discoideum...
  89. ncbi Identification of the missing component in the mitochondrial benzamidoxime prodrug-converting system as a novel molybdenum enzyme
    Antje Havemeyer
    Pharmaceutical Institute, Department of Pharmaceutical and Medicinal Chemistry, Christian Albrechts University of Kiel, 24118 Kiel, Germany
    J Biol Chem 281:34796-802. 2006
    ..A protein fraction free from cytochrome b5 and cytochrome b5 reductase was purified, enhancing 250-fold the minor benzamidoxime-reductase activity catalyzed by the membrane-..
  90. ncbi Diaphanous 1 and 2 regulate smooth muscle cell differentiation by activating the myocardin-related transcription factors
    Dean P Staus
    Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
    Arterioscler Thromb Vasc Biol 27:478-86. 2007
    ..The objective of the present study was to determine the contributions of the RhoA effectors, diaphanous 1 and 2 (mDia1 and mDia2), to this regulatory mechanism...
  91. ncbi Structure and properties of the recombinant NADH-cytochrome b5 reductase of Physarum polycephalum
    Terumi Ikegami
    Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Japan
    Biosci Biotechnol Biochem 71:783-90. 2007
    ..These results suggest that the Physarum enzyme plays an important role in the organism...
  92. pmc Microarray reveals complement components are regulated in the serum-deprived rat retinal ganglion cell line
    Abdelnaby Khalyfa
    Department of Anatomical Sciences and Neurobiology, University of Louisville, School of Medicine, Louisville, KY 40292, USA
    Mol Vis 13:293-308. 2007
    ..The aim of this study was to generate a genome-wide gene expression of RGC-5 following serum deprivation and to identify candidate genes that may be involved in the signal transduction pathways...
  93. pmc Cytochrome b5 and NADH cytochrome b5 reductase: genotype-phenotype correlations for hydroxylamine reduction
    James C Sacco
    Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Pharmacogenet Genomics 20:26-37. 2010
    NADH cytochrome b5 reductase (b5R) and cytochrome b5 (b5) catalyze the reduction of sulfamethoxazole hydroxylamine (SMX-HA), which can contribute to sulfonamide hypersensitivity, to the parent drug sulfamethoxazole...
  94. pmc Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain
    Bin Deng
    Department of Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Biol Chem 285:30181-91. 2010
    ..Trp(114) is an invariant surface residue in all known Ncb5or orthologs but appears not to contribute to electron transfer from the b(5)R domain to the b(5) domain...
  95. pmc MicroRNA regulation of glycoprotein B5R in oncolytic vaccinia virus reduces viral pathogenicity without impairing its antitumor efficacy
    Mina Hikichi
    Core Facility for Therapeutic Vectors, Institute of Medical Science, University of Tokyo, Tokyo, Japan
    Mol Ther 19:1107-15. 2011
    ..Since the deletion of viral glycoprotein B5R not only decreases viral pathogenicity but also impairs the oncolytic activity of vaccinia virus, we used miRNA-..
  96. pmc Safety and immunogenicity of LC16m8, an attenuated smallpox vaccine in vaccinia-naive adults
    Jeffrey S Kennedy
    Wadsworth Center, New York State Department of Health, Albany, NY, USA
    J Infect Dis 204:1395-402. 2011
    LC16m8 is an attenuated cell culture-adapted Lister vaccinia smallpox vaccine missing the B5R protein and licensed for use in Japan.
  97. doi Recovery and molecular characterization of live Camelpox virus from skin 12 months after onset of clinical signs reveals possible mechanism of virus persistence in herds
    A A Yousif
    Central Biotechnology Laboratory, College of Veterinary Medicine and Animal Resources, King Faisal University, Al Ahsaa, Saudi Arabia
    Vet Microbiol 159:320-6. 2012
    ..Virus identity was confirmed by sequence analysis of the CMLV A33R, A27L, B5R, and L1R orthologue genes...
  98. doi Sequence and phylogenetic analysis of host-range (E3L, K3L, and C7L) and structural protein (B5R) genes of buffalopox virus isolates from buffalo, cattle, and human in India
    Bidhan Ch Bera
    Veterinary Type Culture Collection, National Research Centre on Equines, Sirsa Road, Hisar, Haryana, India
    Virus Genes 45:488-98. 2012
    ..genes: E3L, K3L, and C7L are essential for virus replication by preventing interferon resistance, whereas B5R is essential for spread of the virus and evasion from the host's immune response as in VACV...
  99. doi Transient neonatal cyanosis associated with a new Hb F variant: Hb F viseu
    Celeste Bento
    Unidade de Anemias Congénitas, Centro Hospitalar e Universitário de Coimbra Coimbra, Portugal
    J Pediatr Hematol Oncol 35:e77-80. 2013
    Neonatal cyanosis in healthy newborns can be associated either with methemoglobin due to cytochrome b5 reductase deficiency or to M-hemoglobin, a group of hemoglobin variants resulting from mutations in the globin chain genes...
  100. pmc Endothelial cell expression of haemoglobin α regulates nitric oxide signalling
    Adam C Straub
    Robert M Berne Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia 22908, USA
    Nature 491:473-7. 2012
    ..NO signalling, and this signalling is shut off when Hb α is reduced to the Fe(2+) state by endothelial cytochrome b5 reductase 3 (CYB5R3, also known as diaphorase 1)...

Research Grants25

  1. Biochemical Characterization of Cytochrome b5 Reductase in Candida albicans
    MARY JOLENE PATRICIA HOLLOWAY; Fiscal Year: 2010
    ..One such enzyme, cytochrome b5 reductase (cb5r), has a high pharmacological significance owing to its role in fatty acid elongation, ergosterol (..
  2. CYTOCHROME B5--A CASE STUDY IN MOLECULAR RECOGNITION
    Mario Rivera; Fiscal Year: 1999
    ..Interestingly, the E 1/2 of NADH-cytochrome b5 reductase is -88 mV, and -102 mV in the presence of NAD...
  3. David Ross; Fiscal Year: 2014
    ..handling in cells stably transfected with the one electron reductases cytochrome P450 reductase or cytochrome b5 reductase which cause increased quinone one electron redox cycling and increased reactive oxygen generation...
  4. IRON AND OXYGEN CHEMISTRIES IN SIPUNCULAN BLOOD
    DONALD KURTZ; Fiscal Year: 1992
    ..Item (ii) refers to a system consisting of transfer of electrons in the sequence, NADH leads to NADH- cytochrome b5 reductase leads to cytochrome b5 leads to methemerythrin...
  5. Nitogen reduction and xenobiotic response
    Lauren A Trepanier; Fiscal Year: 2010
    ..We have recently shown that hydroxylamines are detoxified by the flavoprotein NADH cytochrome b5 reductase (b5R) and the hemeprotein cytochrome b5 (cyt b5), through a novel, direct pathway of xenobiotic reduction...
  6. Thermodynamic Properties of NADH:Nitrate Reductase
    Michael Barber; Fiscal Year: 2005
    ..and mechanistic features with a variety of other flavin-, heme- and Mo-containing proteins including cytochrome b5 reductase, ferredoxin:NADP+ reductase, cytochrome b5 and sulfite oxidase...
  7. ISOLATION OF ELONGATION ENZYMES & SITE OF SYNTHESIS
    DOMINICK CINTI; Fiscal Year: 1991
    ..of the beta-ketoacyl CoA reductase since this step requires electron input from cytochrome b5 via NADH cytochrome b5 reductase or NADPH cytochrome P-450 reductase...
  8. Dose Optimization, Production & Tox Testing of Replicon-Vaccines for Smallpox
    Kurt Kamrud; Fiscal Year: 2009
    ..Preliminary studies have identified four candidate vaccinia virus (VACV) genes A33R, B5R, A27L and L1R (4pox) that when expressed in animals, elicit a protective immune response...
  9. Development of an VEE Replicon Vaccine Against Smallpox
    Kurt Kamrud; Fiscal Year: 2005
    ..The specific aims are (1) to construct VRP vaccines expressing VACV L1R, A33R, B5R and A27L genes (4pox) using GMP-compliant plasmid vectors and generate VACV-specific reagents, (2) to evaluate ..
  10. POXVIRUS SCR-CONTAINING PROTEINS AS THERAPEUTIC TARGETS
    STUART ISAACS; Fiscal Year: 2003
    ..The vaccinia complement-control protein (VCP) and the extracellular enveloped virus (EEV)-specific B5R protein both contain short consensus repeat (SCR) units present in complement regulatory proteins...
  11. MECHANISMS OF NATURAL KILLER CELL ACTIVATION
    Daniel D Billadeau; Fiscal Year: 2012
    ..direct interaction with ITAM-containing receptor complexes on NK cells;(c) The actin-nucleating formins FMNL1 and DIA1 regulate the initial phase of MTOC polarization that precedes the final translocation of the vesicles to the plams ..
  12. VACCINIA B5R PROTEIN IN SPREAD IMMUNE EVASION, VACCINES
    STUART ISAACS; Fiscal Year: 2005
    ..The vaccinia B5R gene encodes a 42-kDa protein found on the membranes of extracellular virions and infected cells...
  13. OXYGEN SENSING AND SIGNAL TRANSDUCTION
    H Bunn; Fiscal Year: 2003
    ..We have discovered three novel genes that have strong homology to cytochrome b5 reductase. One of these genes is of particular interest since it encodes a 488 residue fusion protein which, on the ..
  14. ACTIN AND MICROTUBULE-BASED MECHANISMS FOR FUNCTIONS OF NK LYMPHOID CELLS
    John A Cooper; Fiscal Year: 2013
    ..We found that Arp2/3 is necessary for assembly of the actin-based lytic synapse and that the formin Dia1 appears to capture microtubule plus ends at the lytic synapse, which is required for the polarized secretion of ..
  15. Development of therapeutic antibodies for vaccinia virus
    Gary Cohen; Fiscal Year: 2003
    ..These are: 1) to express and characterize VV envelope proteins LIR, A33R and B5R in a baculovirus expression system and to prepare mouse and rabbit antibodies to those proteins; 2) to develop and ..
  16. LIPID METABOLISM AND MEMBRANE STRUCTURE AND FUNCTION
    Michael Glaser; Fiscal Year: 1990
    ..The properties of D-Beta-hydroxybutyrate dehydrogenase, cytochrome b5 and cytochrome b5 reductase will be examined in order to determine how lipids interact with them and affect their activity...
  17. STRUCTURE-FUNCTION STUDIES ON MICROSOMAL MEMBRANES
    PHILIPP STRITTMATTER; Fiscal Year: 1992
    ..transport enzymes of endoplasmic reticulum involving cytochrome b5 interactions with two electron donors, cytochrome b5 reductase and cytochrome P-450 reductase and mainly one of a number of electron acceptors, stearyl-CoA desaturase...
  18. PRODRUGS TARGETING HYPOXIC TUMOR CELLS
    Alan Sartorelli; Fiscal Year: 2000
    ..A determination of the role of NADPH:cytochrome C (P450) reductase, NADH:cytochrome b5 reductase and DT-diaphorase in the reductive action of the prodrug, employing purified enzymes and cells ..
  19. Bruce L Goode; Fiscal Year: 2016
    ..of the actin and microtubule cytoskeletons are coordinated by a group of three interacting mammalian proteins: APC, Dia1, and EB1...
  20. Dev. of Optimized/Adjuvanted Smallpox DNA Vaccine (Gene Gun)
    T C Wu; Fiscal Year: 2009
    ..Among the poxvirus-encoded proteins, four membrane proteins (A33R, B5R, L1R and A27L) (called 4-pox vaccine) have been shown to generate protective neutralizing antibody responses and ..
  21. Cocktail of Neutralizing MAbs for Smallpox Prevention
    JEFFREY NORDSTROM; Fiscal Year: 2009
    ..The specific targets of the MAbs in this proposal are B5R and A33R (EEV-specific proteins) and A27L and L1R (IMV-specific proteins)...
  22. Structural and Functional Analysis of Oxygen Sensor
    Hao Zhu; Fiscal Year: 2003
    ..We have cloned and characterized a novel cytochrome b-type NAD(P)H oxidase (b5/b5R) which appears to be a good candidate oxygen sensor...
  23. VACCINIA B5R PROTEIN IN DISSEMINATION AND IMMUNE EVASION
    STUART ISAACS; Fiscal Year: 2001
    DESCRIPTION: The B5R protein encoded by vaccinia virus appears to be a multifunctional protein which affects viral pathogenesis as well as the formation of the extracellular enveloped (EEV) forms of the virus...
  24. Molecular and Cellular Biology of Lipids
    Charles Martin; Fiscal Year: 2007
    ..Proper facilities are available to accommodate handicapped individuals who will be considered equally in the selection of attendees and speakers. ..
  25. REGULATION OF FATTY ACID METABOLISM
    Charles Martin; Fiscal Year: 2002
    ..abstract_text> ..