CYP11A1

Summary

Gene Symbol: CYP11A1
Description: cytochrome P450 family 11 subfamily A member 1
Alias: CYP11A, CYPXIA1, P450SCC, cholesterol side-chain cleavage enzyme, mitochondrial, cholesterol 20-22 desmolase, cholesterol monooxygenase (side-chain cleaving), cytochrome P450 11A1, cytochrome P450 family 11 subfamily A polypeptide 1, cytochrome P450(scc), cytochrome P450, subfamily XIA (cholesterol side chain cleavage), steroid 20-22-lyase
Species: human

Top Publications

  1. ncbi Microsatellite polymorphism of steroid hormone synthesis gene CYP11A1 is associated with advanced prostate cancer
    Teruaki Kumazawa
    Department of Urology, Akita University School of Medicine, Akita 010 8543, Japan
    Int J Cancer 110:140-4. 2004
  2. ncbi The transcriptional regulating protein of 132 kDa (TReP-132) enhances P450scc gene transcription through interaction with steroidogenic factor-1 in human adrenal cells
    Florence Gizard
    Oncology and Molecular Endocrinology Research Center, Laval University, Quebec GIK 7P4, Canada
    J Biol Chem 277:39144-55. 2002
  3. ncbi Transcription of cholesterol side-chain cleavage cytochrome P450 in the placenta: activating protein-2 assumes the role of steroidogenic factor-1 by binding to an overlapping promoter element
    Micha Ben-Zimra
    Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel 91904
    Mol Endocrinol 16:1864-80. 2002
  4. ncbi A systematic assessment of common genetic variation in CYP11A and risk of breast cancer
    Veronica Wendy Setiawan
    Departments of Preventive Medicine and Urology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Cancer Res 66:12019-25. 2006
  5. pmc Haplotype analysis of CYP11A1 identifies promoter variants associated with breast cancer risk
    Brian L Yaspan
    Department of Cancer Biology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, TN 37232 0275, USA
    Cancer Res 67:5673-82. 2007
  6. ncbi Transcriptional regulation of human CYP11A1 in gonads and adrenals
    Ing Cherng Guo
    Department of Veterinary Medicine, College of Bio Resources and Agriculture, National Taiwan University, Taipei, Taiwan
    J Biomed Sci 14:509-15. 2007
  7. doi The presence of the CYP11A1 (TTTTA)6 allele increases the risk of biochemical relapse in organ confined and low-grade prostate cancer
    Teja Celhar
    Faculty of Pharmacy, University of Ljubljana, Askerceva 7, SI 1000 Ljubljana, Slovenia
    Cancer Genet Cytogenet 187:28-33. 2008
  8. pmc Genetic variation in CYP11A1 and StAR in relation to endometrial cancer risk
    Kathryn Terry
    Department of Epidemiology, Harvard School of Public Health, Boston, MA, United States
    Gynecol Oncol 117:255-9. 2010
  9. ncbi Population-based case-control study of CYP11A gene polymorphism and breast cancer risk
    Wei Zheng
    Drpartment of Medicine and Vanderbilt Ingram Cancer Centr, Vanderbilt University, Nashville, TN, USA
    Cancer Epidemiol Biomarkers Prev 13:709-14. 2004
  10. ncbi LBP proteins modulate SF1-independent expression of P450scc in human placental JEG-3 cells
    Ningwu Huang
    Department of Pediatrics, University of California, San Francisco, San Francisco, California 94143 0978, USA
    Mol Endocrinol 19:409-20. 2005

Research Grants

  1. Role of Benzodiazepine Receptor in Leydig Cell Function
    Vassilios Papadopoulos; Fiscal Year: 2011
  2. STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEIN
    HIMANGSHU BOSE; Fiscal Year: 2002
  3. STAR AND SF-1: ESTROGEN SYNTHESIS AND BREAST CANCER RISK
    Robert Haile; Fiscal Year: 2000
  4. HORMONAL REGULATION OF STEROIDOGENESIS
    Synthia Mellon; Fiscal Year: 1990
  5. ROLES OF STAR IN REGULATED STEROIDOGENESIS
    Keith Parker; Fiscal Year: 2007
  6. SF-1 INDEPENDENT TRANSCRIPTIONAL REGULATION OF P450SCC
    Ningwu Huang; Fiscal Year: 2005
  7. MOLECULAR REGULATION OF STEROIDOGENESIS
    WALTER MILLER; Fiscal Year: 1999
  8. Irina A Pikuleva; Fiscal Year: 2016
  9. Molecular Mechanisms of Atrazine in Altering the Endocrine Gene Network
    Holly A Ingraham; Fiscal Year: 2010
  10. THE MOLECULAR BASIS OF ADRENARCHE: DISSECTION OF P450C17
    Richard Auchus; Fiscal Year: 2000

Detail Information

Publications359 found, 100 shown here

  1. ncbi Microsatellite polymorphism of steroid hormone synthesis gene CYP11A1 is associated with advanced prostate cancer
    Teruaki Kumazawa
    Department of Urology, Akita University School of Medicine, Akita 010 8543, Japan
    Int J Cancer 110:140-4. 2004
    ..The human CYP11A1 gene (CYP11A1) encodes the P450scc enzyme, which mediates the first step in sex steroid hormone synthesis...
  2. ncbi The transcriptional regulating protein of 132 kDa (TReP-132) enhances P450scc gene transcription through interaction with steroidogenic factor-1 in human adrenal cells
    Florence Gizard
    Oncology and Molecular Endocrinology Research Center, Laval University, Quebec GIK 7P4, Canada
    J Biol Chem 277:39144-55. 2002
    The human P450scc gene is regulated by the tissue-specific orphan nuclear receptor, steroidogenic factor-1 (SF-1), which plays a key role in several physiologic processes including steroid synthesis, adrenal and gonadal development, and ..
  3. ncbi Transcription of cholesterol side-chain cleavage cytochrome P450 in the placenta: activating protein-2 assumes the role of steroidogenic factor-1 by binding to an overlapping promoter element
    Micha Ben-Zimra
    Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel 91904
    Mol Endocrinol 16:1864-80. 2002
    ..cell, express high levels of the key steroidogenic enzyme, cholesterol side-chain cleavage cytochrome P450 (P450scc)...
  4. ncbi A systematic assessment of common genetic variation in CYP11A and risk of breast cancer
    Veronica Wendy Setiawan
    Departments of Preventive Medicine and Urology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    Cancer Res 66:12019-25. 2006
    b>CYP11A catalyzes the rate-limiting step in the biosynthesis of sex-steroid hormones...
  5. pmc Haplotype analysis of CYP11A1 identifies promoter variants associated with breast cancer risk
    Brian L Yaspan
    Department of Cancer Biology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, TN 37232 0275, USA
    Cancer Res 67:5673-82. 2007
    The CYP11A1 gene encodes the cholesterol side chain cleavage enzyme that catalyzes the initial and rate-limiting step of steroidogenesis...
  6. ncbi Transcriptional regulation of human CYP11A1 in gonads and adrenals
    Ing Cherng Guo
    Department of Veterinary Medicine, College of Bio Resources and Agriculture, National Taiwan University, Taipei, Taiwan
    J Biomed Sci 14:509-15. 2007
    The CYP11A1 gene encodes the cholesterol side-chain cleavage enzyme, also termed cytochrome P450scc, which catalyzes the conversion of cholesterol to pregnenolone in the first step of steroid biosynthesis in mitochondria...
  7. doi The presence of the CYP11A1 (TTTTA)6 allele increases the risk of biochemical relapse in organ confined and low-grade prostate cancer
    Teja Celhar
    Faculty of Pharmacy, University of Ljubljana, Askerceva 7, SI 1000 Ljubljana, Slovenia
    Cancer Genet Cytogenet 187:28-33. 2008
    The involvement of the CYP11A1 gene in the synthesis of androgens makes it a compelling candidate for various hormone-dependent diseases, including prostate cancer...
  8. pmc Genetic variation in CYP11A1 and StAR in relation to endometrial cancer risk
    Kathryn Terry
    Department of Epidemiology, Harvard School of Public Health, Boston, MA, United States
    Gynecol Oncol 117:255-9. 2010
    Together, steroidogenic acute regulator (StAR) and the cholesterol side chain cleavage enzyme (P450scc), which is encoded by CYP11A1, mediate the initial and rate-limiting step in steroidogenesis...
  9. ncbi Population-based case-control study of CYP11A gene polymorphism and breast cancer risk
    Wei Zheng
    Drpartment of Medicine and Vanderbilt Ingram Cancer Centr, Vanderbilt University, Nashville, TN, USA
    Cancer Epidemiol Biomarkers Prev 13:709-14. 2004
    The CYP11A gene encodes the cholesterol side-chain cleavage enzyme (P450scc) that catalyzes the first and rate-limiting step for the biosynthesis of sex hormones...
  10. ncbi LBP proteins modulate SF1-independent expression of P450scc in human placental JEG-3 cells
    Ningwu Huang
    Department of Pediatrics, University of California, San Francisco, San Francisco, California 94143 0978, USA
    Mol Endocrinol 19:409-20. 2005
    The cholesterol side-chain cleavage enzyme, P450scc, initiates biosynthesis of all steroid hormones...
  11. ncbi Adrenodoxin (Adx) and CYP11A1 (P450scc) induce apoptosis by the generation of reactive oxygen species in mitochondria
    Evi Derouet-Hümbert
    Department of Biochemistry, Building No 9 2, Saarland University, D 66041 Saarbrucken, Germany
    Biol Chem 386:453-61. 2005
    ..g., CYP11A1 (P450 side chain cleavage, P450scc). It is known that the latter two are able to generate reactive oxygen species (ROS) in vitro ...
  12. pmc The osteogenic transcription factor runx2 controls genes involved in sterol/steroid metabolism, including CYP11A1 in osteoblasts
    Nadiya M Teplyuk
    Department of Cell Biology, Worcester, Massachusetts 01655 0106, USA
    Mol Endocrinol 23:849-61. 2009
    ..Cyp11a1 (P450scc) encodes an approximately 55-kDa mitochondrial enzyme that catalyzes side-chain cleavage of cholesterol and is ..
  13. ncbi The orphan nuclear receptor, liver receptor homolog-1, regulates cholesterol side-chain cleavage cytochrome p450 enzyme in human granulosa cells
    Joung W Kim
    Division of Reproductive Endocrinology and Infertility, Cedars Medical Center, 1400 NW 12th Avenue, East Building 4th floor, Miami, Florida 33136, USA
    J Clin Endocrinol Metab 90:1678-85. 2005
    ..The first step in human ovarian steroidogenesis is catalyzed by cholesterol side-chain cleavage cytochrome P450 (CYP11A1) enzyme...
  14. ncbi Organ-specific and non-organ-specific autoantibodies in children and young adults with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED)
    R Perniola
    Paediatric Unit, Vito Fazzi Regional Hospital, Lecce, Italy
    Eur J Endocrinol 143:497-503. 2000
    ....
  15. ncbi Site-directed mutagenesis of cytochrome P450scc. II. Effect of replacement of the Arg425 and Arg426 residues on the structural and functional properties of the cytochrome P450scc
    T N Azeva
    Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus
    Biochemistry (Mosc) 66:564-75. 2001
    ..and site-directed mutagenesis, we identified surface exposed positively charged residues of cytochrome P450scc which might be important for interaction with adrenodoxin...
  16. ncbi Transcriptional regulation of P450scc gene expression in neural and steroidogenic cells: implications for regulation of neurosteroidogenesis
    P Zhang
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco 94143, USA
    Mol Endocrinol 9:1571-82. 1995
    Regulation of steroidogenesis in classic endocrine tissues is mediated by transcriptional regulation of the P450scc gene, which encodes the first and rate-limiting cholesterol side-chain cleavage enzyme...
  17. ncbi Induction of Ad4BP/SF-1, steroidogenic acute regulatory protein, and cytochrome P450scc enzyme system expression in newly established human granulosa cell lines
    K Hosokawa
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Endocrinology 139:4679-87. 1998
    ..Expression of the electron carrier adrenodoxin (ADX), which is a part of the cytochrome P450scc enzyme system, was very low in nonstimulated cells but was dramatically elevated in FK- and 8-Br-cAMP-stimulated ..
  18. ncbi Light and electron microscopic immunohistochemistry of the localization of adrenal steroidogenic enzymes
    K Ishimura
    Department of Anatomy, Tokushima University, School of Medicine, Japan
    Microsc Res Tech 36:445-53. 1997
    ..Light microscopical investigations showed that cytochromes P450 of cholesterol side-chain cleavage enzyme (P450scc) and of 11 beta-hydroxylase (P45011 beta), 3 beta-hydroxysteroid dehydrogenase/ delta 5-4 isomerase (3 beta HSD), ..
  19. ncbi Steroidogenic enzyme P450c17 is expressed in the embryonic central nervous system
    N A Compagnone
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco 94143, USA
    Endocrinology 136:5212-23. 1995
    ..previously demonstrated that the messenger RNAs (mRNA) for the cholesterol side-chain cleavage enzyme, cytochrome P450scc, and one form of 11 beta-hydroxylase, cytochrome P450c11 beta, are regionally expressed in the adult rat brain...
  20. ncbi Molecular and cellular mechanisms used in the acute phase of stimulated steroidogenesis
    M Thomson
    Department of Biological Sciences, University of Western Sydney Nepean, Kingswood, Australia
    Horm Metab Res 30:16-28. 1998
    ..transported through the contact sites to the inner membrane and on reaching cytochrome P450 side chain cleavage (P450scc), cholesterol is converted to pregnenolone...
  21. pmc Introduction of a gonadotropin receptor expression plasmid into immortalized granulosa cells leads to reconstitution of hormone-dependent steroidogenesis
    B S Suh
    Department of Hormone Research, Weizmann Institute of Science, Rehovot
    J Cell Biol 119:439-50. 1992
    ..These cells lost their response to gonadotropins, but expressed the cytochrome P450scc mitochondrial system enzymes and produced progesterone and 20 alpha-hydroxy-4-pregnan-3-one (20 alpha-OH-P) upon ..
  22. ncbi Progesterone synthesis by the human placenta
    Robert C Tuckey
    Biochemistry and Molecular Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia
    Placenta 26:273-81. 2005
    ..step of placental progesterone synthesis is the conversion of cholesterol to pregnenolone by cytochrome P450scc (CYP11A1) in placental mitochondria in a reaction requiring electrons delivered via adrenodoxin reductase and adrenodoxin...
  23. ncbi The pharmacology of neurosteroidogenesis
    E Costa
    Fidia Georgetown Institute for the Neurosciences, Georgetown University Medical School, Washington, DC 20007
    J Steroid Biochem Mol Biol 49:385-9. 1994
    ..tissues including glial cells, the conversion of cholesterol into pregnenolone is catalyzed by the cytochrome P450scc located in the inner mitochondrial membrane...
  24. ncbi Screening for mutations in the steroidogenic acute regulatory protein and steroidogenic factor-1 genes, and in CYP11A and dosage-sensitive sex reversal-adrenal hypoplasia gene on the X chromosome, gene-1 (DAX-1), in hyperandrogenic hirsute women
    R M Calvo
    Department of Endocrinology, , Madrid 28034, Spain
    J Clin Endocrinol Metab 86:1746-9. 2001
    ..and the adrenal is the conversion of cholesterol into pregnenolone by cholesterol side-chain cleavage enzyme (P450scc), encoded by the gene CYP11A, after cholesterol is introduced into the mitochondria by the steroidogenic acute ..
  25. pmc The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism
    Andrzej Slominski
    Department of Pathology and Laboratory Medicine, University of Tennessee, Health Science Center, Memphis, TN 38163, USA
    FEBS J 272:4080-90. 2005
    We show that cytochrome P450scc (CYP11A1) in either a reconstituted system or in isolated adrenal mitochondria can metabolize vitamin D3...
  26. ncbi Effects of polychlorinated biphenyl (Aroclor 1254) on steroidogenesis and antioxidant system in cultured adult rat Leydig cells
    Palaniappan Murugesan
    Department of Endocrinology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India
    J Endocrinol 192:325-38. 2007
    ..LH receptors and activities of steroidogenic enzymes, such as cytochrome P(450) side-chain cleavage enzyme (P450scc), 3beta-HSD, and 17beta-hydroxysteroid dehydrogenase (17beta-HSD)...
  27. doi Gene expression profiling during spermatogenesis in early maturing male Atlantic salmon parr testes
    Gersende Maugars
    Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences, SE 901 83 Umea, Sweden
    Gen Comp Endocrinol 159:178-87. 2008
    ..homolog), steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), 3beta-hydroxysteroid dehydrogenase/Delta(5)-Delta(4)-isomerase (3beta-HSD), cytochrome P450 17alpha-hydroxylase/..
  28. ncbi Neurosteroid biosynthesis: genes for adrenal steroidogenic enzymes are expressed in the brain
    S H Mellon
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco 94143 0556
    Brain Res 629:283-92. 1993
    ..We detected mRNAs for adrenodoxin, P450scc (cholesterol side-chain cleavage enzyme) and P450c11 beta (11 beta-hydroxylase) but not for P450c17 (17 alpha-..
  29. ncbi Polypyrimidine tract-binding protein-associated splicing factor is a negative regulator of transcriptional activity of the porcine p450scc insulin-like growth factor response element
    R J Urban
    Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555 1060, USA
    Mol Endocrinol 14:774-82. 2000
    The porcine P-450 cholesterol side-chain cleavage enzyme gene (P450scc) contains a 30-bp region [insulin-like growth factor response element (IGFRE)] that mediates insulin-like growth factor I (IGF-I)-stimulated gene expression and binds ..
  30. ncbi Comparative structural and immunochemical characterization of recombinant and natural cytochrome p450scc (CYPXIAI)
    G I Lepesheva
    Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, ul Zhodinskaya 5 2, Minsk, 220141 Belarus
    Biochemistry (Mosc) 63:224-34. 1998
    Optimization of the conditions for heterologous expression of recombinant cytochrome P450scc in E. coli provided an expression level of about 420 nmoles of cytochrome P450scc per liter of bacterial culture...
  31. pmc Optimizing the conditions of a multiple reaction monitoring assay for membrane proteins: quantification of cytochrome P450 11A1 and adrenodoxin reductase in bovine adrenal cortex and retina
    Wei Li Liao
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    Anal Chem 82:5760-7. 2010
    ..on the optimization of sample preparation conditions for the quantification of two membrane proteins, cytochrome P450 11A1 (CYP11A1) and adrenodoxin reductase (AdR)...
  32. ncbi The role of tumor necrosis factor-alpha in the regulation of mouse Leydig cell steroidogenesis
    Y Xiong
    Department of Physiology and Biophysics, University of Illinois, Chicago 60612 7342
    Endocrinology 132:2438-44. 1993
    ..both basal and 8-Br-cAMP-stimulated testosterone production, as well as cholesterol side-chain cleavage enzyme (P450scc) and 17 alpha-hydroxylase/C17,20 lyase (P450c17), were investigated. Treatment of Leydig cells with 0.1, 1...
  33. ncbi Reconstruction of mitochondrial cytochrome P450-dependent steroid hydroxylases in artificial phospholipid membranes: studies of cytochrome P450scc topology by limited proteolysis
    A V Krivosheev
    Institute of Bioorganic Chemistry, Academy of Sciences of Belarus, Minsk, Belarus
    Biochemistry (Mosc) 62:1064-73. 1997
    Highly purified cytochrome P450scc from bovine adrenal cortex mitochondria was inserted in artificial phospholipid membranes prepared from phosphatidylcholine to study the main principles of its membrane organization in the model system...
  34. ncbi Expression and regulation of adrenodoxin and P450scc mRNA in rodent tissues
    S H Mellon
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco 94143 0556
    DNA Cell Biol 10:339-47. 1991
    ..cholesterol through its three protein components: adrenodoxin reductase, adrenodoxin, and the terminal oxidase, P450scc. We have previously shown that P450scc mRNA is regulated by tropic hormones and cAMP by a cycloheximide-..
  35. ncbi Submitochondrial distribution of three key steroidogenic proteins (steroidogenic acute regulatory protein and cytochrome p450scc and 3beta-hydroxysteroid dehydrogenase isomerase enzymes) upon stimulation by intracellular calcium in adrenal glomerulosa cel
    N Cherradi
    Department of Internal Medicine, Faculty of Medicine, University Hospital, CH 1211 Geneva 14, Switzerland
    J Biol Chem 272:7899-907. 1997
    ..In contrast, neither Ca2+ nor Ang II had any effect on the submitochondrial distribution of cytochrome P450scc and 3beta-hydroxysteroid dehydrogenase isomerase...
  36. ncbi Curvature properties of novel forms of phosphatidylcholine with branched acyl chains
    R M Epand
    Department of Biochemistry, McMaster University Health Sciences Center, Hamilton, Canada
    Eur J Biochem 267:2909-15. 2000
    ..been shown to have marked stimulatory effects on the side-chain cleavage activity of cytochrome P450SCC (CYP11A1), an enzyme of the inner mitochondrial membrane...
  37. ncbi Expression of SCC in ovarian granulosa cells and cultured cells, induced rapid structural changes in mitochondria
    T Yoshinaga-Hirabayashi
    Department of Anatomy, Osaka University Medical School, Japan
    Ital J Anat Embryol 106:51-7. 2001
    In this study, the relation of P450scc expression and mitochondrial ultrastructure was examined in rat granulosa cells at the time of ovulation, and in the NIH/3T3 cells...
  38. ncbi Semi-quantitative RT-PCR analysis of environmental influence on P450scc and PNMT mRNA expression in rat adrenal glands
    K Morita
    Department of Pharmacology, Tokushima University School of Medicine, Kuramoto, Japan
    Life Sci 70:73-80. 2001
    ..conditions on the steady-state levels of mRNAs encoding cholesterol side-chain cleavage enzyme (cytochrome P450scc) and phenylethanolamine N-methyltransferase (PNMT) in adrenal glands was examined to investigate the ..
  39. pmc A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1)
    O Guryev
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9038, USA
    Proc Natl Acad Sci U S A 100:14754-9. 2003
    ..In this article, we describe in vitro experiments using purified mitochondrial cytochrome P450scc (CYP11A1) reconstituted with the iron-sulfer protein, adrenodoxin, and the flavoprotein, adrenodoxin reductase, ..
  40. ncbi Sex differences, developmental changes, response to injury and cAMP regulation of the mRNA levels of steroidogenic acute regulatory protein, cytochrome p450scc, and aromatase in the olivocerebellar system
    Esteban Lavaque
    Instituto Cajal, C S I C, E 28002 Madrid, Spain
    J Neurobiol 66:308-18. 2006
    ..factors, such as the steroidogenic acute regulatory protein (StAR), cytochrome P450 side chain cleavage (P450scc), and aromatase, at this neural site has been reported...
  41. doi Leydig cell re-generation and expression of cell signaling molecules in the germ cell-free testis
    P J O'Shaughnessy
    Division of Cell Sciences, Institute of Comparative Medicine, University of Glasgow Veterinary School, Bearsden Road, Glasgow G61 1QH, UK
    Reproduction 135:851-8. 2008
    ..were marked declines in levels of Leydig cell-specific mRNA transcripts coding for steroidogenic enzymes cytochrome P450 11a1 (Cyp11a1), cytochrome P450 17a1 (Cyp17a1), 3beta-hydroxysteroid dehydrogenase type 1 (Hsd3b1), 17beta-..
  42. ncbi Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement
    Hwei Jan Hsu
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan
    Nature 439:480-3. 2006
    ..which is produced from cholesterol by the steroidogenic enzyme Cyp11a1 (cholesterol side-chain cleavage enzyme, P450scc), functions in promoting cell migration during epiboly...
  43. pmc An alternative pathway of vitamin D metabolism. Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2
    Andrzej Slominski
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    FEBS J 273:2891-901. 2006
    ..an alternative, hydroxylating pathway for the metabolism of vitamin D2 in a cytochrome P450 side chain cleavage (P450scc; CYP11A1) reconstituted system...
  44. pmc cAMP regulates P450scc gene expression by a cycloheximide-insensitive mechanism in cultured mouse Leydig MA-10 cells
    S H Mellon
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco 94143
    Proc Natl Acad Sci U S A 86:7775-9. 1989
    ..ovine luteinizing hormone, forskolin, or 8-bromoadenosine 3',5'-cyclic monophosphate, stimulated cytochrome P450scc mRNA accumulation 1.5- to 3-fold and progesterone secretion 10- to 100-fold in MA-10 cells...
  45. ncbi The cDNA cloning and tissue expression of the cytochrome P450scc from Syrian hamster (Mesocricetus auratus)
    F Vilchis
    Department of Reproductive Biology, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Vasco de Quiroga No 15, Mexico DF 14000, Mexico
    Gen Comp Endocrinol 126:279-86. 2002
    In this study we report the cloning and sequencing of a cDNA for cholesterol side chain cleavage cytochrome P450scc from Syrian hamster adrenal glands...
  46. ncbi Regulation of the human P450scc gene by steroidogenic factor 1 is mediated by CBP/p300
    D Monte
    Laboratory of Molecular Endocrinology, CHUL Research Centre and Laval University, Sainte Foy, Quebec, G1V 4G2, Canada
    J Biol Chem 273:4585-91. 1998
    Regulation of the human CYP11A gene encoding cytochrome P450scc, which catalyzes the first step of steroid synthesis, is regulated by many trans-acting transcription factors including steroidogenic factor 1 (SF-1)...
  47. pmc Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc
    Robert C Tuckey
    School of Biomolecular, Biomedical and Chemical Sciences, The University of Western Australia, Crawley, WA, Australia
    FEBS J 275:2585-96. 2008
    Cytochrome P450scc (CYP11A1) can hydroxylate vitamin D3 to produce 20-hydroxyvitamin D3 and other poorly characterized hydroxylated products...
  48. pmc Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cyclodextrin
    Robert C Tuckey
    School of Biomedical, Biomolecular and Chemical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
    Int J Biochem Cell Biol 40:2619-26. 2008
    Vitamin D3 can be hydroxylated sequentially by cytochrome P450scc (CYP11A1) producing 20-hydroxyvitamin D3, 20,23-dihydroxyvitamin D3 and 17,20,23-trihydroxyvitamin D3...
  49. ncbi Covalently crosslinked complexes of bovine adrenodoxin with adrenodoxin reductase and cytochrome P450scc. Mass spectrometry and Edman degradation of complexes of the steroidogenic hydroxylase system
    E C Müller
    Max Delbruck Centrum fur Molekulare Medizin, Germany
    Eur J Biochem 268:1837-43. 2001
    ..electrons from the FAD of adrenodoxin reductase to the heme iron of the catalytically active cytochrome P450 (P450scc)...
  50. ncbi Immunohistochemical identification of cells expressing steroidogenic enzymes cytochrome P450scc and P450 aromatase in taste buds of rat circumvallate papillae
    Kuniaki Toyoshima
    Division of Oral Histology and Neurobiology, Department of Biosciences, Kyushu Dental College, Kitakyushu, Japan
    Arch Histol Cytol 70:215-24. 2007
    ..localizations and patterns of expression of key enzymes for steroidogenesis, cytochrome P450 side-chain-cleavage (P450scc), and P450 aromatase in the taste buds of rat circumvallate papillae, using immunoblot analyses and ..
  51. pmc Metabolism of 1alpha-hydroxyvitamin D3 by cytochrome P450scc to biologically active 1alpha,20-dihydroxyvitamin D3
    Robert C Tuckey
    School of Biomedical, Biomolecular and Chemical Sciences, M310, The University of Western Australia, Crawley, WA 6009, Australia
    J Steroid Biochem Mol Biol 112:213-9. 2008
    Cytochrome P450scc (CYP11A1) metabolizes vitamin D3 to 20-hydroxyvitamin D3 as the major product, with subsequent production of dihydroxy and trihydroxy derivatives...
  52. ncbi Hormonal and developmental regulation of adrenodoxin messenger ribonucleic acid in steroidogenic tissues
    R Voutilainen
    Department of Pediatrics, University of California, San Francisco 94143
    J Clin Endocrinol Metab 66:383-8. 1988
    ..in steroidogenesis as an electron transport intermediate for mitochondrial cytochromes P450, including P450scc, the cholesterol side-chain cleavage enzyme...
  53. pmc Structural basis for three-step sequential catalysis by the cholesterol side chain cleavage enzyme CYP11A1
    Natalia Mast
    Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 286:5607-13. 2011
    Mitochondrial cytochrome P450 11A1 (CYP11A1 or P450 11A1) is the only known enzyme that cleaves the side chain of cholesterol, yielding pregnenolone, the precursor of all steroid hormones...
  54. pmc Partial defect in the cholesterol side-chain cleavage enzyme P450scc (CYP11A1) resembling nonclassic congenital lipoid adrenal hyperplasia
    Taninee Sahakitrungruang
    Department of Pediatrics, University of California San Francisco, San Francisco, California 94143 0978, USA
    J Clin Endocrinol Metab 96:792-8. 2011
    The cholesterol side-chain cleavage enzyme (P450scc), encoded by the CYP11A1 gene, converts cholesterol to pregnenolone to initiate steroidogenesis...
  55. ncbi Characterization of the rat Star gene that encodes the predominant 3.5-kilobase pair mRNA. ACTH stimulation of adrenal steroids in vivo precedes elevation of Star mRNA and protein
    N Ariyoshi
    Department of Pharmacology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 273:7610-9. 1998
    ..protein (STAR) participates in steroidogenesis through the mitochondrial transfer of cholesterol to cytochrome P450scc. The rat adrenal Star gene is transcribed as a 3. 5-kilobase pair (kb) and 1...
  56. ncbi A novel zinc finger protein TReP-132 interacts with CBP/p300 to regulate human CYP11A1 gene expression
    F Gizard
    Oncology and Molecular Endocrinology Research Center, Laval University, Quebec G1K 7P4, Canada
    J Biol Chem 276:33881-92. 2001
    The human CYP11A1 gene is expressed specifically in steroidogenic tissues and encodes cytochrome P450scc, which catalyzes the first step in steroid synthesis...
  57. ncbi Homozygous mutation of P450 side-chain cleavage enzyme gene (CYP11A1) in 46, XY patient with adrenal insufficiency, complete sex reversal, and agenesis of corpus callosum
    Hessa al Kandari
    King Fahad Hospital, King Abdul Aziz Medical City, P O Box 22490, Pediatric Department 1510, Riyadh 11426, Kingdom of Saudi Arabia
    J Clin Endocrinol Metab 91:2821-6. 2006
    ..Defective enzyme activity leads to the deficiency of all steroid hormones, including progesterone, which is essential to sustain term pregnancy...
  58. ncbi Comparative study of topogenesis of cytochrome P450scc (CYP11A1) and its hybrids with adrenodoxin expressed in Escherichia coli cells
    A A Vinogradova
    Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
    Biochemistry (Mosc) 72:208-14. 2007
    Hybrid proteins consisting of the mature form of cytochrome P450scc (mP) and adrenodoxin (Ad), attached to either the NH2- or COOH-terminus (Ad-mP and mP-Ad, respectively), were expressed in E. coli...
  59. pmc Neurosteroids and epileptogenesis in the pilocarpine model: evidence for a relationship between P450scc induction and length of the latent period
    Giuseppe Biagini
    Dipartimento di Scienze Biomediche, Universita di Modena e Reggio Emilia, Modena, Italy
    Epilepsia 50:53-8. 2009
    Cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) catalyzes the initial step in the biosynthesis of neurosteroids within the brain...
  60. ncbi Transcriptional regulation of the human FTZ-F1 gene encoding Ad4BP/SF-1
    K Oba
    The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Maidashi Higashi-ku, Fukuoka 812-8582, Japan
    J Biochem 128:517-28. 2000
    ..of the hFTZ-F1 gene was in striking contrast to the regulation of steroidogenic CYP genes, such as the human CYP11A gene, in which the proximal promoter activity is Ad4BP-dependent and the transactivation by Ad4BP is silenced by ..
  61. ncbi DAX-1 blocks steroid production at multiple levels
    E Lalli
    , Centre National de la Recherche Scientifique, , , Strasbourg, France
    Endocrinology 139:4237-43. 1998
    ..This is explained by the repression of P450scc and 3beta-HSD expression, in addition to StAR...
  62. pmc Human adrenodoxin reductase: two mRNAs encoded by a single gene on chromosome 17cen----q25 are expressed in steroidogenic tissues
    S B Solish
    Department of Pediatrics, University of California, San Francisco 94143
    Proc Natl Acad Sci U S A 85:7104-8. 1988
    ..The expression of the adrenodoxin reductase gene may be stimulated by pituitary tropic hormones acting through cAMP, but its response is quantitatively much less than the responses of P450scc and adrenodoxin.
  63. ncbi Vasoactive intestinal peptide-induced expression of cytochrome P450 cholesterol side-chain cleavage and 17 alpha-hydroxylase enzyme activity in hen granulosa cells
    A L Johnson
    Department of Animal Science, Rutgers, State University of New Jersey, New Brunswick 08903
    Biol Reprod 51:327-33. 1994
    ..whether vasoactive intestinal peptide (VIP) can regulate expression of cytochrome P450 side-chain cleavage (P450scc) and P450 17 alpha-hydroxylase (P450 17 alpha-OH) mRNA levels and enzyme activity in granulosa cells from ..
  64. ncbi Inhibition of steroidogenesis by luteal cells of early pregnancy in the rat in response to in vitro administration of a gonadotropin-releasing hormone agonist
    R K Srivastava
    Department of Physiology, Morehouse School of Medicine, Atlanta, GA 30310 1495
    J Steroid Biochem Mol Biol 49:73-9. 1994
    ..a concomitant increase in luteal lipid droplets and decreases in the luteal cytochrome P450 side chain cleavage (P450scc) enzyme and its mRNA content...
  65. ncbi A StAR search: implications in controlling steroidgenesis
    D M Stocco
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock 79430, USA
    Biol Reprod 56:328-36. 1997
    ..the level of cholesterol delivery to the inner mitochondrial membrane, the site of the P450 side-chain cleavage (P450scc) enzyme that will convert it to pregnenolone, the first steroid synthesized...
  66. ncbi Changes in androgen-producing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra
    Mohammad Ashraful Alam
    Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Motobu, Okinawa, Japan
    Mol Reprod Dev 73:206-14. 2006
    ..We examined the dynamics of steroidogenic enzymes immunolocalization, viz cholesterol side-chain cleavage (P450scc), biomarker of steroids and cytochrome P45011beta-hydroxylase (P45011beta), downstream to 11-KT production, ..
  67. ncbi Liver X receptor activation enhances cholesterol loss from the brain, decreases neuroinflammation, and increases survival of the NPC1 mouse
    Joyce J Repa
    Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, Texas 75390 9151, USA
    J Neurosci 27:14470-80. 2007
    ..genes associated with the formation of 24(S)-hydroxycholesterol or neurosteroids such as CYP46A1, 3alphaHSD, and CYP11A1. However, levels of the sterol transporters ABCA1 and ATP-binding cassette transporter G1 were increased...
  68. doi Evidence for an inhibitory role of bone morphogenetic protein(s) in the follicular-luteal transition in cattle
    Amjad R Kayani
    School of Biological Sciences, University of Reading, Whiteknights, Reading, UK
    Reproduction 137:67-78. 2009
    ..of STAR mRNA and raised 'basal' CYP17A1 mRNA level in theca-lutein cells without affecting expression of CYP11A1 or hydroxy-Delta-5-steroid dehydrogenase, 3 beta- and steroid Delta-isomerase 1 (HSD3B1)...
  69. doi Efficacy of exemestane, a new generation of aromatase inhibitor, on sex differentiation in a gonochoristic fish
    Sabina Ruksana
    Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Sesoko 3422, Motobu, Okinawa 905 0227, Japan
    Comp Biochem Physiol C Toxicol Pharmacol 152:69-74. 2010
    ..fish showed strong gonadal expression of the steroidogenic enzymes P450 cholesterol-side chain-cleavage enzyme (P450scc), 3beta-hydroxysteroid dehydrogenase (3beta-HSD), and cytochrome P450 aromatase (P450arom)...
  70. doi Citrinin reduces testosterone secretion by inducing apoptosis in rat Leydig cells
    Shuqiang Liu
    College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, People s Republic of China
    Toxicol In Vitro 26:856-61. 2012
    ..Western blot assays also showed that CTN decreased expression of three key enzymes (P450scc, 3β-HSD-1, and StAR) of testosterone production...
  71. pmc Caffeine-induced activated glucocorticoid metabolism in the hippocampus causes hypothalamic-pituitary-adrenal axis inhibition in fetal rats
    Dan Xu
    Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, China
    PLoS ONE 7:e44497. 2012
    ..expression of fetal adrenal steroid acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme (P450scc), as well as the level of fetal adrenal endogenous corticosterone (CORT), were all significantly decreased after ..
  72. doi 22-NBD-cholesterol as a novel fluorescent substrate for cholesterol-converting oxidoreductases
    Yaroslav V Faletrov
    Research Institute for Physical Chemical Problems, Belarusian State University, Leningradskaya Str 14, 220030 Minsk, Belarus
    J Steroid Biochem Mol Biol 134:59-66. 2013
    ..22-NBD-cholesterol), a common fluorescent sterol analog, binds into active sites of bovine cytochrome P450scc and microbial cholesterol dehydrogenases (CHDHs) and then undergoes regiospecific oxidations by these enzymes...
  73. doi The effect of mitotane on viability, steroidogenesis and gene expression in NCI‑H295R adrenocortical cells
    Tomasz P Lehmann
    Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poznań 60 781, Poland
    Mol Med Rep 7:893-900. 2013
    ..Concomitantly, mitotane resulted in decreased mRNA levels of two cytochromes P450 (CYP11A1 and CYP17A1), mRNAs encoding proteins involved in the synthesis of cortisol and DHEAS...
  74. pmc Foetal exposure to Panax ginseng extract reverts the effects of prenatal dexamethasone in the synthesis of testosterone by Leydig cells of the adult rat
    Maria I Wanderley
    Department of Physiology and Pharmacology, Universidade Federal de Pernambuco, Recife, Brazil
    Int J Exp Pathol 94:230-40. 2013
    ..StAR immunoexpression in Leydig cells was not modified by prenatal exposure to DEXA, GE or DEXA + GE. P450scc and glucocorticoid receptor immunoexpression was higher in offspring exposed to DEXA in comparison with the ..
  75. pmc Growth Arrest Specific-1 (GAS1) Is a C/EBP Target Gene That Functions in Ovulation and Corpus Luteum Formation in Mice
    Yi A Ren
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas
    Biol Reprod 94:44. 2016
    ..Interestingly, levels of mRNA for a number of genes (Cyp11a1, Star, Wnt4, Prlr, Cd52, and Sema3a) associated with luteinization are decreased in corpora lutea of Gas1 mutant ..
  76. doi Assessment of the endocrine-disrupting effects of short-chain chlorinated paraffins in in vitro models
    Quan Zhang
    College of Environment, Zhejiang University of Technology, Hangzhou 310032, China College of Environment and Resource Sciences, Zhejiang University, Hangzhou 310058, China
    Environ Int 94:43-50. 2016
    ..StAR, 17βHSD, CYP11A1, CYP11B1, CYP19 and CYP21 were upregulated in a concentration-dependent manner by all chemicals...
  77. ncbi Tumor necrosis factor-alpha inhibits leydig cell steroidogenesis through a decrease in steroidogenic acute regulatory protein expression
    C Mauduit
    INSERM U407, Centre Hospitalier Lyon Sud, Pierre Benite, France
    Endocrinology 139:2863-8. 1998
    ..an observation suggested that the cytokine may affect cholesterol transport and/or availability to cytochrome P450scc in the mitochondria...
  78. ncbi Transcriptional regulation of the rat steroidogenic acute regulatory protein gene by steroidogenic factor 1
    T W Sandhoff
    Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa 33606, USA
    Endocrinology 139:4820-31. 1998
    ..to facilitate cholesterol transport to the inner mitochondrial membrane-bound P450 side-chain cleavage enzyme (P450scc), the first enzymatic step in the steroid hormone biosynthetic pathway...
  79. ncbi Comparative structural-functional characterization of recombinant and natural adrenodoxin. Interaction with cytochrome P450scc
    G I Lepesheva
    Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus
    Biochemistry (Mosc) 64:1079-88. 1999
    ..The interactions of the natural and recombinant adrenodoxins with cytochrome P450scc have been studied and compared with respect to: the efficiency of their enzymatic reduction of cytochrome P450scc ..
  80. ncbi Conformational dynamics and molecular interaction reactions of recombinant cytochrome p450scc (CYP11A1) detected by fluorescence energy transfer
    G I Lepesheva
    Institute of Bioorganic Chemistry National Academy of Sciences of Belarus, 220141, Minsk, Belarus
    Biochim Biophys Acta 1434:31-43. 1999
    Bovine adrenocortical cytochrome P450scc (P450scc) was expressed in Escherichia coli and purified as the substrate bound, high-spin complex (16.7 nmol of heme per mg of protein, expression level in E. coli about 400-700 nmol/l)...
  81. ncbi Cellular distribution and bioactivity of the key steroidogenic enzyme, cytochrome P450side chain cleavage, in sensory neural pathways
    Christine Patte-Mensah
    Laboratoire de Neurophysiologie Cellulaire et Intégrée, Unité Mixte de Recherche 7519 Centre National de la Recherche Scientifique, Universite Louis Pasteur, 67084 Strasbourg Cedex, France
    J Neurochem 86:1233-46. 2003
    ..We studied the presence and activity of cytochrome P450side chain cleavage (P450scc) in rat pain pathways...
  82. ncbi Transcriptional regulation of P450scc gene expression in the embryonic rodent nervous system
    Fabian Hammer
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, 513 Parnassus Avenue, Box 0556, San Francisco, CA 94143 0556, USA
    Endocrinology 145:901-12. 2004
    ..with the conversion of cholesterol to pregnenolone, catalyzed by mitochondrial P450 side-chain cleavage enzyme (P450scc)...
  83. pmc The potential function of steroid sulphatase activity in steroid production and steroidogenic acute regulatory protein expression
    Teruo Sugawara
    Department of Biochemistry, Hokkaido University Graduate School of Medicine, Kita ku, Kita 15, Nishi 7, Sapporo 060 8638, Japan
    Biochem J 380:153-60. 2004
    ..Steroidogenic activities of COS-1 cells that had been co-transfected with a vector for the cholesterol P450scc (cytochrome P450 side-chain-cleavage enzyme) system, named F2, a StAR expression vector (pStAR), and an STS ..
  84. pmc A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin
    Andrzej Slominski
    Department of Pathology and Laboratory Medicine, University of Tennessee, Health Science Center, Memphis, TN, USA
    Eur J Biochem 271:4178-88. 2004
    ..from progesterone, we now show widespread cutaneous expression of the full cytochrome P450 side-chain cleavage (P450scc) system required for the intracellular catalytic production of pregnenolone, i.e...
  85. ncbi Testicular toxicity of profenofos in matured male rats
    Gihan Gamal Moustafa
    Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita ku, Sapporo, 060 0818, Japan
    Arch Toxicol 81:875-81. 2007
    ..to steroidogenesis including cytochrome P450 17A1 (CYP17A1), steroidogenic acute regulatory protein (StAR) and CYP11A1 were significantly increased...
  86. pmc Metabolism of vitamin d2 to 17,20,24-trihydroxyvitamin d2 by cytochrome p450scc (CYP11A1)
    Minh N Nguyen
    School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    Drug Metab Dispos 37:761-7. 2009
    As well as catalyzing the conversion of cholesterol to pregnenolone for steroid synthesis, cytochrome P450scc (P450scc) can also metabolize vitamins D2 (D2) and D3 (D3)...
  87. doi Fetal testis dysgenesis and compromised Leydig cell function in Tgfbr3 (beta glycan) knockout mice
    Mai A Sarraj
    Prince Henry s Institute of Medical Research, Clayton, Victoria, Australia
    Biol Reprod 82:153-62. 2010
    ..The expression levels of the Leydig cell markers Insl3, Cyp17a1, Cyp11a1, Star, and Hsd3b1 were reduced in knockout testis compared to wild-type testis, beginning at 12.5 dpc...
  88. pmc Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells
    Bernard P Schimmer
    Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, ON, Canada M5G 1L6
    Mol Cell Endocrinol 336:85-91. 2011
    ..The knockdown of SF-1 did not affect the accumulation of Cyp11a1 transcripts even though the amount of SF-1 bound to the proximal promoter of the gene was reduced to background ..
  89. doi Influence of standing estrus before an injection of GnRH during a beef cattle fixed-time AI protocol on LH release, subsequent concentrations of progesterone, and steriodogenic enzyme expression
    S D Fields
    Department of Animal and Range Sciences, South Dakota State University, Brookings, SD 57007, USA
    Domest Anim Endocrinol 42:11-9. 2012
    ..addition, there was no effect of estrus, follicle size, or CL weight on luteal expression of LH receptor, StAR, CYP11A1, or 3βHSD. However, there was a correlation between follicle size and CL weight (P = 0.01; R(2) = 0...
  90. pmc Pregnane glycosides interfere with steroidogenic enzymes to down-regulate corticosteroid production in human adrenocortical H295R cells
    Slavko Komarnytsky
    Biotech Center, SEBS, Rutgers University, New Brunswick, NJ, USA
    J Cell Physiol 228:1120-6. 2013
    ..Incubation of pregnane glycosides for 24 h had no effect on mRNA transcripts of CYP11A1, CYP21A1, CYP11B1 cytochrome enzymes and steroidogenic acute regulatory protein (StaR) protein, yet resulted in ..
  91. doi Reversible infertility in a liver receptor homologue-1 (LRH-1)-knockdown mouse model
    Han Gerrits
    Merck Sharp and Dohme Research Laboratories, Women s Health Department, Molenstraat 110, 5342 CC, Oss, The Netherlands Corresponding author Email
    Reprod Fertil Dev 26:293-306. 2014
    ..in line with the observed decrease in plasma progesterone levels, key steroid biosynthesis genes, namely Star, Cyp11a1, Hsd3b and Scarb1, were downregulated in LRH-1-knockdown ovaries...
  92. doi Dose-dependent alterations in gene expression and testosterone production in fetal rat testis after exposure to di-n-hexyl phthalate
    Anne Marie Saillenfait
    Institut National de Recherche et de Securite, Vandoeuvre, France
    J Appl Toxicol 33:1027-35. 2013
    ..e. SR-B1, StAR, P450scc, 3βHSD and P450c17). These inhibitions were dose dependent...
  93. pmc Wnt signaling inhibits adrenal steroidogenesis by cell-autonomous and non-cell-autonomous mechanisms
    Elisabeth M Walczak
    Cellular and Molecular Biology Graduate Program E M W, G D H and Program in Biomedical Sciences N B, University of Michigan Medical School Center for Cancer Biostatistics R K, Department of Biostatistics, University of Michigan School of Public Health Department of Internal Medicine I F, G D H, Division of Metabolism, Endocrinology and Diabetes Department of Internal Medicine S M H, D M W, Division of Molecular Medicine and Genetics and Endocrine Oncology Program G D H, Comprehensive Cancer Center, University of Michigan, Ann Arbor, Michigan 48109
    Mol Endocrinol 28:1471-86. 2014
    ..caused decreased corticosterone release consistent with the observed reduced transcription of steroidogenic genes Cyp11a1, Cyp11b1, Star, and Mc2r...
  94. doi Exercise prevents the increased anxiety-like behavior in lactational di-(2-ethylhexyl) phthalate-exposed female rats in late adolescence by improving the regulation of hypothalamus-pituitary-adrenal axis
    Dean Chuan Wang
    Department of Sports Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan Electronic address
    Horm Behav 66:674-84. 2014
    ..levels of ACTH and corticosterone, the expressions of adrenal enzymes cholesterol side-chain cleavage enzyme (CYP11A1) and cytochrome P-450 11β-hydroxylase (CYP11B1), and the expression of hypothalamic glucocorticoid receptors (GR)..
  95. pmc Altered theca and cumulus oocyte complex gene expression, follicular arrest and reduced fertility in cows with dominant follicle follicular fluid androgen excess
    Adam F Summers
    Department of Animal Science, University of Nebraska Lincoln, Lincoln, Nebraska, United States of America
    PLoS ONE 9:e110683. 2014
    ..Theca cell mRNA expression of LHCGR, GATA6, CYP11A1, and CYP17A1 was greater in High A4 cows...
  96. pmc Effect of azaline B on follicular development and functions in the hamster
    Prabuddha Chakraborty
    Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA
    Mol Cell Endocrinol 400:1-9. 2015
    ..The level of Cyp11a1 mRNA increased markedly in LH- or LH + FSH-treated hamsters, whereas FSH with or without LH upregulated ..
  97. pmc Sex-dependent effects of microcystin-LR on hypothalamic-pituitary-gonad axis and gametogenesis of adult zebrafish
    Wanjing Liu
    College of Fisheries, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, People s Republic of China
    Sci Rep 6:22819. 2016
    ..were positively correlated with the number of post-vitellogenic oocytes, while PC1 (gnrh2, lhβ, erβ, fshr, cyp11a and 17βhsd) were positively correlated with the number of spermatozoa...
  98. doi MCL1 is a key regulator of steroidogenesis in mouse Leydig cells
    Li Guang-Yu
    First Affiliated Hospital of Guangxi Medical University, Guangxi, P R China
    Mol Reprod Dev 83:226-35. 2016
    ..The mRNA abundance of the steroidogenic genes Star, Cyp11a1, Cyp17a1, Hsd3b1, Srd5a, and the luteinizing hormone/choriogonadotropin receptor Lhcgr were significantly reduced ..
  99. pmc Transcriptome Analysis of Bovine Ovarian Follicles at Predeviation and Onset of Deviation Stages of a Follicular Wave
    Pengfei Li
    College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China
    Int J Genomics 2016:3472748. 2016
    ..KEGG pathway analysis revealed two upregulated genes in ODF1 versus PDF1, CYP11A1, and CYP19A1, which are important genes in the steroid hormone biosynthesis pathway...
  100. doi Oleic acid induces specific alterations in the morphology, gene expression and steroid hormone production of cultured bovine granulosa cells
    Vengala Rao Yenuganti
    Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology FBN, 18196 Dummerstorf, Germany
    Gen Comp Endocrinol 232:134-44. 2016
    ..reduced the transcript abundance of the gonadotropin hormone receptors, FSHR and LHCGR, steroidogenic genes STAR, CYP11A1, HSD3B1 and CYP19A1, the cell cycle regulator CCND2, but not of the proliferation marker PCNA...
  101. pmc Modulation of gonadotrophin induced steroidogenic enzymes in granulosa cells by d-chiroinositol
    Sandro Sacchi
    Mother Infant Department, University of Modena and Reggio Emilia, Via Del Pozzo 41, 41100, Modena, Italy
    Reprod Biol Endocrinol 14:52. 2016
    ..regulation, cytochrome P450 family 19 subfamily A member 1 (CYP19A1) and cytochrome P450 side-chain cleavage (P450scc) in primary cultures of human granulosa cells (hGCs)...

Research Grants55

  1. Role of Benzodiazepine Receptor in Leydig Cell Function
    Vassilios Papadopoulos; Fiscal Year: 2011
    ..the sequence of molecular events involved in the transfer of cholesterol mobilized by StAR to the cytochrome P450scc via PBR...
  2. STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEIN
    HIMANGSHU BOSE; Fiscal Year: 2002
    ..of all steroid hormones is the conversion of cholesterol to pregnenolone by the mitochondrial cytochrome P450scc system...
  3. STAR AND SF-1: ESTROGEN SYNTHESIS AND BREAST CANCER RISK
    Robert Haile; Fiscal Year: 2000
    ..StAR facilities transport of cholesterol across the mitochondrial membrane to cytochrome P450scc which converts cholesterol to the steroid precursor, pregnenolone...
  4. HORMONAL REGULATION OF STEROIDOGENESIS
    Synthia Mellon; Fiscal Year: 1990
    ..Employing probes for the cholesterol side chain cleavage enzyme (P450scc), 21-hydroxylase (P450c21), and 17-hydroxylase (P450c17), we propose to use in situ hybridization histochemistry ..
  5. ROLES OF STAR IN REGULATED STEROIDOGENESIS
    Keith Parker; Fiscal Year: 2007
    ..predominantly reflects increased delivery of cholesterol substrate to the cholesterol side-chain cleavage enzyme (Cyp11a) in the inner mitochondrial membrane...
  6. SF-1 INDEPENDENT TRANSCRIPTIONAL REGULATION OF P450SCC
    Ningwu Huang; Fiscal Year: 2005
    DESCRIPTION (provided by applicant) The cholesterol side-chain cleavage enzyme, cytochrome P450scc, initiates the biosynthesis of all steroid hormones and is the rate-limiting and hormonally regulated step...
  7. MOLECULAR REGULATION OF STEROIDOGENESIS
    WALTER MILLER; Fiscal Year: 1999
    ..The first is the cholesterol side chain cleavage enzyme (P450scc), which carries out the rate-limiting step in the production of steroid hormones...
  8. Irina A Pikuleva; Fiscal Year: 2016
    ..Outside the liver, two P450s are particularly important, CYP46A1 and CYP11A1, which metabolize the majority of cholesterol in the brain and steroidogenic tissues, respectively...
  9. Molecular Mechanisms of Atrazine in Altering the Endocrine Gene Network
    Holly A Ingraham; Fiscal Year: 2010
    ..upregulate SF-1 targets and other genes critical for steroid synthesis and reproduction, including Cyp19A1, StAR, Cyp11A1, hCG, FSTL3, LHss, INH1, 1GSU, and 11ss-HSD2...
  10. THE MOLECULAR BASIS OF ADRENARCHE: DISSECTION OF P450C17
    Richard Auchus; Fiscal Year: 2000
    ..rise in DHEA-S production that defines adrenarche cannot occur unless pregnenolone, generated by the action of P450scc on cholesterol in the adrenal mitochondria, can escape the action of 3beta-hydroxysteroid dehydrogenase/delta5-4-..
  11. Placental progesterone biosynthesis in preterm pregnancy
    Eileen Wang; Fiscal Year: 2006
    ..P450 side chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase type I (3bHSD-1) act to convert cholesterol to P...
  12. Mechanism of Metformin Action in Normal and PCOS Theca Cells
    Jan M McAllister; Fiscal Year: 2012
    ..the role(s) of LKB1, AMPK, and the salt inducible kinases, SIK1 and SIK2, in metformin inhibition of CYP17 and CYP11A1 gene expression and androgen biosynthesis in normal and PCOS theca cells propagated in long-term culture...
  13. Novel Biosynthethic Pathway for Secosteroids and the Skin
    Andrzej T Slominski; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The discovery of new secosteroidogenic pathways, regulated by P450scc represents a fundamental and unexpected advance in skin biology, endocrinology, and pharmacotherapy of vitamin D3...
  14. Leptin and hypothalamo-pituitary-adrenal function in the long-term hypoxic fetus
    Charles A Ducsay; Fiscal Year: 2013
    ..LTH fetus also adapts in response to LTH including a reduced expression of key steroidogenic enzymes (CYP17 and CYP11A1). Cumulatively, these seemingly contradictory adaptations in the hypothalamo-pituitary vs...
  15. Genes, Hormones and Breast Cancer Risk
    Veronica Wendy Setiawan; Fiscal Year: 2010
    ..are associated with plasma hormone levels, 2) characterize genetic variation of estrogen biosynthesis genes (CYP11A, CYP17, CYP19, HSD3B1, HSD3B2, HSD17B1, HSD17B2, and SHBG) using a two-pronged approach (putative functional ..
  16. Mechanism of action of steroidogenic acute regulatory protein (StAR)
    Himangshu S Bose; Fiscal Year: 2013
    ..mechanism facilitates the movement of cholesterol from the OMM to IMM, thus regulating the flow of substrate to P450scc for steroidogenesis...
  17. Genetic Polymorphisms Benign Prostatic Hyperplasia
    ROSEBUD ROBERTS; Fiscal Year: 2004
    ..We will evaluate the CYP11A1, CYP17, CYP19, 3Beta hydroxysteroid dehydrogenase, 17BETA hydroxysteroid dehydrogenase types 2,3, 5, and SRD5A2 ..
  18. Genes, Hormones & Environment in an Ovarian Cancer Model
    Daniel Cramer; Fiscal Year: 2007
    ..By affecting steroid production, genetic variants of 3 and 17betaHSD, CYP11A, CYP17, and CYP 19 may affect risk for ovarian cancer...
  19. 17 alpha-Hydroxylase Expression in Human Ovarian Cells
    Jan M McAllister; Fiscal Year: 2010
    ..directly affect the down stream signaling components of each homologous pathway to the extent that CYP17 and CYP11A1 gene expression, and androgen biosynthesis are up-regulated in PCOS theca cells...
  20. BREAST CANCER AND GENE POLYMORPHISMS IN CHINESE WOMEN
    Chu Chen; Fiscal Year: 2003
    ..whether most known polymorphisms of a number of genes involved in the biosynthesis and catabolism of estrogens (CYP11a, CYP17, EDH17beta2, CYP1A1, CYP1A2, CYP1B1, COMT), and those for the androgen receptor, estrogen receptor and IGF-..
  21. CORPUS LUTEUM SCP2 EXPRESSION PHOSPHORYLATION & FUNCTION
    MARK MC LEAN; Fiscal Year: 2000
    In the corpus luteum, cholesterol transport to the mitochondrial P450 side chain cleavage enzyme (P450scc) is thought to be the rate limiting step in steroid production...
  22. EPIGENETIC EFFECT OF METHOXYCHLOR ON OVARIAN DEVELOPMENT
    Mehmet Uzumcu; Fiscal Year: 2007
    ..DNA methylation and critical gene expression (e.g., StAR, P450scc, LHR) for adult ovarian function will be examined...
  23. Modulation by dioxin of ovarian estrogen synthesis
    REINHOLD HUTZ; Fiscal Year: 2002
    ..g., by modifying levels of P450 side-chain cleavage (SCC, CYP11A) or aromatase (CYP19) mRNA, protein and activity; and has no effect on estrogen-metabolizing enzymes (CYP1A1, 1B1)...
  24. FUNCTION AND STRUCTURE OF HUMAN FERREDOXIN
    LARRY VICKERY; Fiscal Year: 1992
    ..and 3) identify residues involved in binding and electron transfer with NADPH- oxidoreductase and cytochromes P450scc and P450-11Beta...
  25. MECHANISMS OF IGF I STIMULATED OVARIAN STEROIDOGENESIS
    Randall Urban; Fiscal Year: 2000
    ..IGF-I regulates the rate-limiting enzyme in the steroidogenic pathway, P-450 cholesterol side-chain cleavage (P450scc). Dr...
  26. Role of CRH in skin physiology and pathology
    Andrzej Slominski; Fiscal Year: 2004
    ..g., melanocytes and keratinocytes, and test the role of CRH/CRH-R1 axis in the regulation of human hair growth. ..
  27. STEROID HYDROXYLASES IN NORMAL AND ABNORMAL ADRENALS
    BOYD HARDING; Fiscal Year: 1980
    ..Their function will be studied by determining their relationship to protein kinases and phosphodiesterases and by examining their effects on cholesterol transport to mitochondrial P450scc and upon the catalytic activity of this enzyme.
  28. ANDROGEN PRODUCTION IN ADRENARCHE AND PCOS
    WALTER MILLER; Fiscal Year: 2006
    ..abstract_text> ..
  29. Gene Interactions, Estrogen, and Risk of Breast Cancer
    Kathleen Egan; Fiscal Year: 2006
    ..Genes included in this proposal are involved at key branch points in estrogen synthesis (STAR, CYP11A1, HSD 3B, CYP17, CYP19, HSD17B , TNF, IL6, PPAR G, STS), in steroid signaling (ESR1, ESR2, PGR, SHBG, AIB1), and ..
  30. ESTROGEN, DIET, GENETICS AND ENDOMETRIAL CANCER
    Sara Olson; Fiscal Year: 2005
    ..This study will provide data on genetic susceptibility in endometrial cancer in conjunction with established risk factors, as well as the first epidemiologic data on the more lethal serous and clear cell tumors. ..
  31. The function of Runx1 in the ovary
    Misung Jo; Fiscal Year: 2007
    ..Such knowledge can be applied for promoting or inhibiting these critical facets of ovarian physiology, thereby allowing us to better manage fertility, infertility, and ovarian-based disorders. [unreadable] [unreadable]..
  32. Can acclimation to toxins lead to sustained resistance?
    ALAN KOLOK; Fiscal Year: 2002
    ..We hypothesize that the results of the larval survivability tests will depend upon the exposure history (acclimation) of the parents, not the relative genetic susceptibility of the parents ..
  33. Human Endometrial Nitric Oxide: Regulation and Function
    Omid Khorram; Fiscal Year: 2003
    ..This pilot study should shed light on regulation and function of human endometrial NO pathway, an uninvestigated area of research with profound clinical significance. ..
  34. Developmental Differences in Brain Androgen Synthesis
    Sarah London; Fiscal Year: 2004
    ..They will also indicate potential regulatory mechanisms for androgen synthesis in other species, including humans. ..
  35. OVARIAN LDL RECEPTOR AND STAR GENE REGULATION
    HOLLY LAVOIE; Fiscal Year: 2003
    ..These studies will promote our understanding of how multiple genes are coordinated by different trophic factors in the ovary during follicular differentiation and add to our understanding of mechanisms of infertility. ..
  36. REGULATION OF REPRODUCTION
    Synthia Mellon; Fiscal Year: 2005
    ..abstract_text> ..
  37. REGULATION OF REPRODUCTIVE PROCESSES
    DOUGLAS STOCCO; Fiscal Year: 2003
    ..abstract_text> ..
  38. PERINATAL HYPOXIA: ADRENOCORTICAL-METABOLIC ADAPTATIONS
    Hershel Raff; Fiscal Year: 2006
    ..Characterization of the hypothalamic-pituitary-adrenal and metabolic adaptations to perinatal hypoxia can lead to new diagnostic approaches and therapies to minimize morbidity and mortality. ..
  39. ROLE OF THE FAS ANTIGEN IN OVARIAN CELL APOPTOSIS
    SUSAN QUIRK; Fiscal Year: 2004
    ..Understanding pathways that promote or prevent susceptibility of ovarian cells to apoptosis may lead to development of novel therapies for ovarian and other cancers. ..
  40. Cyclic Testosterone on muscle function in older men
    Randall Urban; Fiscal Year: 2009
    ..This innovative change in testosterone administration would result in greater gains in strength with fewer side effects. ..
  41. The Nashville Breast Health Study
    Wei Zheng; Fiscal Year: 2009
    ..Studies investigating gene-gene and gene-environment interaction could provide valuable information in identifying high-risk individuals for designing cost-effective preventive strategies for breast cancer. ..
  42. Fusion Proteins As Probes of P450 Structure and Function
    Richard Auchus; Fiscal Year: 2004
    ..In addition, this approach might be generally applicable to structural studies of membrane-bound proteins. ..
  43. PSF Regulates the Porcine P450scc IFGRE
    Randall J Urban; Fiscal Year: 2010
    Our work focuses on IGF-I regulation of gene expression for P-450 cholesterol side-chain cleavage (P450scc), the rate-limiting enzyme in the steroidogenic pathway...
  44. GROWTH FACTOR CONTROL OF OVARIAN ANDROGEN BIOSYNTHESIS
    Denis Magoffin; Fiscal Year: 2001
    ..It is hoped that this will provide novel opportunities for developing new treatments for hyperandrogenism. ..
  45. Tumor Markers and Recurrent Adenomas: A Follow-up Study
    Wei Zheng; Fiscal Year: 2006
    ..This study is likely to provide valuable information for identifying high-risk adenoma patients for close surveillance and chemoprevention. ..
  46. MOLECULAR EPIDEMIOLOGIC STUDY OF BREAST CANCER
    Wei Zheng; Fiscal Year: 2009
    ..Results from this study will be valuable in identifying high risk women for primary and secondary prevention of breast cancer. ..
  47. Glucocorticoids and Programming of the Hypertensive Vascular Phenotype
    Omid Khorram; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  48. Post-transcriptional regulation of gene expression by thyroid hormone receptors
    Bin Xu; Fiscal Year: 2008
    ..The findings of TR targeted mRNAs will reveal novel functions of TR to regulate gene expression at post-transcriptional levels related to the brain function and neurodevelopment.. [unreadable] [unreadable]..
  49. The function(s) and regulation of PPARs in the ovary
    Carolyn Komar; Fiscal Year: 2003
    ....
  50. Post-translational regulation of CYP17 activity
    Denis Magoffin; Fiscal Year: 2008
    ..We expect to utilize the molecular model to design new Pharmaceuticals to treat not only ovarian hyperandrogenism, but also other diseases of androgen excess such as prostate cancer and cardiovascular diseases. ..
  51. Cancer Risk Reduction and Diet: A Cohort Study of Women
    Wei Zheng; Fiscal Year: 2007
    ..The results from this study may guide new strategies in the primary prevention of common cancer in both Western and Asian women. ..
  52. Steroidogenic Acute Regulatory Protein's Role in Libido
    Steven King; Fiscal Year: 2004
    ....
  53. Antioxidant Defense System and Leydig Cell Aging
    Haolin Chen; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  54. Insulin signaling in theca cells from polycystic ovaries
    Denis Magoffin; Fiscal Year: 2006
    ..The results of these studies are expected to yield specific molecular targets for novel therapies to treat women with PCOS. ..
  55. Function and Signaling Mechanisms of Leptin Receptors
    Joyce Repa; Fiscal Year: 2005
    ..A better understanding of the signal transduction pathway used by leptin may reveal novel strategies to enhance or inhibit leptin's biological activities for therapeutic purposes. ..