Genomes and Genes
Gene Symbol: CTCF
Description: CCCTC-binding factor
Alias: MRD21, transcriptional repressor CTCF, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor (zinc finger protein), CTCFL paralog
Publications174 found, 100 shown here
- Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2Wei Chi Ku
Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan
Mol Cell Proteomics 8:2034-50. 2009..SNF complex. In addition to previously known functions of AP-4, our data suggest that AP-4 participates in a transcriptional-regulating complex at the HDM2-P2 promoter in response to DNA damage...
- CTCF is a uniquely versatile transcription regulator linked to epigenetics and diseaseR Ohlsson
Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Trends Genet 17:520-7. 2001b>CTCF is an evolutionarily conserved zinc finger (ZF) phosphoprotein that binds through combinatorial use of its 11 ZFs to approximately 50 bp target sites that have remarkable sequence variation...
- CTCF regulates cell cycle progression of alphabeta T cells in the thymusHelen Heath
Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
EMBO J 27:2839-50. 2008The 11-zinc finger protein CCCTC-binding factor (CTCF) is a highly conserved protein, involved in imprinting, long-range chromatin interactions and transcription...
- A loss of insulin-like growth factor-2 imprinting is modulated by CCCTC-binding factor down-regulation at senescence in human epithelial cellsVivian X Fu
Department of Surgery, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53792, USA
J Biol Chem 279:52218-26. 2004..As a mechanism, a 2-fold decrease in the binding of the enhancer-blocking element CCCTC-binding factor (CTCF) within the intergenic IGF2-H19 region was found to underlie this switch to biallelic IGF2 expression in senescent ..
- Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter tts DNA-binding specificityGalina N Filippova
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Cancer Res 62:48-52. 2002b>CTCF is a widely expressed 11-zinc finger (ZF) transcription factor that is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin ..
- YB-1 and CTCF differentially regulate the 5-HTT polymorphic intron 2 enhancer which predisposes to a variety of neurological disordersElena Klenova
Department of Biological Sciences, University of Essex, Essex CO4 3SQ, United Kingdom
J Neurosci 24:5966-73. 2004..Interestingly, the transcription factor CCTC-binding factor (CTCF), previously shown to interact with YB-1, interferes with the ability of the VNTR to support YB-1-directed reporter ..
- An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenesG N Filippova
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
Mol Cell Biol 16:2802-13. 1996We have isolated and analyzed human CTCF cDNA clones and show here that the ubiquitously expressed 11-zinc-finger factor CTCF is an exceptionally highly conserved protein displaying 93% identity between avian and human amino acid ..
- The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activationA A Vostrov
Department of Psychiatry and Behavioral Science, State University of New York, Stony Brook, New York 11794 8101, USA
J Biol Chem 272:33353-9. 1997..gel electrophoresis, partial peptide sequences were obtained that completely matched the transcriptional factor CTCF. This protein is a known regulator of c-myc and lysozyme gene expression, and it binds to a variety of diverse DNA ..
- The protein CTCF is required for the enhancer blocking activity of vertebrate insulatorsA C Bell
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
Cell 98:387-96. 1999..We show that this sequence is the binding site for CTCF, a previously identified eleven-zinc finger DNA-binding protein that is highly conserved in vertebrates...
- An element in the region responsible for premature termination of transcription mediates repression of c-myc gene expression by thyroid hormone in neuroblastoma cellsG Perez-Juste
Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas and Universidad Aut noma de Madrid, 28029 Madrid, Spain
J Biol Chem 275:1307-14. 2000..These studies provide insight into mechanisms by which TR mediate transcriptional repression and contribute to the understanding of the important effects of thyroid hormones on growth and differentiation of neuronal cells...
- Transcriptional repression by the insulator protein CTCF involves histone deacetylasesM Lutz
Genetisches Institut der Justus Liebig Universitat, Heinrich Buff Ring 58 62, 35392 Giessen, Germany
Nucleic Acids Res 28:1707-13. 2000The highly conserved zinc-finger protein, CTCF, is a candidate tumor suppressor protein that binds to highly divergent DNA sequences...
- Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 geneA C Bell
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
Nature 405:482-5. 2000..The activity of this element is dependent upon the vertebrate enhancer-blocking protein CTCF. Methylation of CpGs within the CTCF-binding sites eliminates binding of CTCF in vitro, and deletion of these sites ..
- CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locusA T Hark
Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, New Jersey 08544, USA
Nature 405:486-9. 2000..Furthermore, we show that CTCF, a zinc finger protein implicated in vertebrate boundary function, binds to several sites in the unmethylated ..
- CTCF, a candidate trans-acting factor for X-inactivation choiceWendy Chao
Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
Science 295:345-7. 2002..Here, we identify the insulator and transcription factor, CTCF, as a candidate trans-acting factor for X-chromosome selection...
- Multiple nucleosome positioning sites regulate the CTCF-mediated insulator function of the H19 imprinting control regionMeena Kanduri
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
Mol Cell Biol 22:3339-44. 2002..set the stage for the remarkably precise distribution of the four target sites for the chromatin insulator protein CTCF to nucleosome linker sequences in the H19 ICR...
- Transforming growth factor-beta-induced transcription of the Alzheimer beta-amyloid precursor protein gene involves interaction between the CTCF-complex and SmadsTeralee Burton
The Dr John Foerster Centre for Health Research on Aging St Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, MB, Canada R3E 0W3
Biochem Biophys Res Commun 295:713-23. 2002..This site interacts with the zinc finger nuclear factor CTCF, involved in APP transcriptional activity...
- CTCF maintains differential methylation at the Igf2/H19 locusChristopher J Schoenherr
Department of Cell and Structural Biology, University of Illinois, Urbana Champaign, Urbana, Illinois 61801, USA
Nat Genet 33:66-9. 2003..One candidate regulator of methylation, the zinc-finger protein CTCF, binds to the imprinting control region (ICR) of the genes Igf2 (encoding insulin-like growth factor 2) and H19 (..
- CTCF functions as a critical regulator of cell-cycle arrest and death after ligation of the B cell receptor on immature B cellsChen Feng Qi
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 100:633-8. 2003..b>CTCF is a transcription factor identified as a repressor of MYC recently shown to cause cell growth inhibition...
- CTCF gene mutations in invasive ductal breast cancerSebastian Aulmann
Department of Pathology, University of Heidelberg, Germany
Breast Cancer Res Treat 80:347-52. 2003The CTCF gene encodes for a transcriptional repressor of the c-myc oncogene and has previously been mapped to one of the smallest regions of overlapping interstitial deletions on chromosome 16q22.1 in invasive breast cancer...
- Human beta-globin locus control region HS5 contains CTCF- and developmental stage-dependent enhancer-blocking activity in erythroid cellsKeiji Tanimoto
Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tennoudai 1 1 1, Tsukuba, Ibaraki 305 8577, Japan
Mol Cell Biol 23:8946-52. 2003..describe genetic evidence demonstrating that human HS5 includes enhancer-blocking (insulator) activity that is both CTCF and developmental stage dependent...
- Transgenic RNAi reveals essential function for CTCF in H19 gene imprintingAndrew M Fedoriw
Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 303:238-40. 2004..Insulin-like growth factor 2 expression involves binding of the vertebrate insulator protein, CCCTC binding factor (CTCF), to the maternally hypomethylated differentially methylated domain (DMD)...
- CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across speciesTimur M Yusufzai
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 13:291-8. 2004..Almost all described vertebrate insulators require binding of the regulatory protein CTCF for their activity...
- Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulationWenqiang Yu
Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Nat Genet 36:1105-10. 2004..We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation...
- CTCF regulates growth and erythroid differentiation of human myeloid leukemia cellsVerónica Torrano
Grupo de Biología Molecular del Cáncer, Departamento de Biologia Molecular, Unidad de Biomedicina CSIC, Universidad de Cantabria, 39011 Santander, Spain
J Biol Chem 280:28152-61. 2005b>CTCF is a transcription factor and a candidate tumor suppressor that contains a DNA-binding domain composed of 11 zinc fingers...
- Heightened expression of CTCF in breast cancer cells is associated with resistance to apoptosisFrance Docquier
Department of Biological Sciences, Central Campus, University of Essex, Essex, United Kingdon
Cancer Res 65:5112-22. 2005b>CTCF is a candidate tumor suppressor gene encoding a multifunctional transcription factor...
- CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatinLes J Burke
Institute for Genetics, Justus Liebig Universitaet Giessen, Giessen, Germany
EMBO J 24:3291-300. 2005..For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented...
- The human enhancer blocker CTC-binding factor interacts with the transcription factor KaisoPierre Antoine Defossez
CNRS UMR5161, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 280:43017-23. 2005CTC-binding factor (CTCF) is a DNA-binding protein of vertebrates that plays essential roles in regulating genome activity through its capacity to act as an enhancer blocker...
- Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanismVerónica Torrano
Grupo de Biología Molecular del Cáncer, Departamento de Biologia Molecular, Universidad de Cantabria, 39011 Santander, Spain
J Cell Sci 119:1746-59. 2006Multiple functions have been reported for the transcription factor and candidate tumour suppressor, CTCF. Among others, they include regulation of cell growth, differentiation and apoptosis, enhancer-blocking activity and control of ..
- CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2Sreenivasulu Kurukuti
Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
Proc Natl Acad Sci U S A 103:10684-9. 2006..the H19 imprinting control region (ICR) directs the silencing of the maternally inherited Igf2 allele through a CTCF-dependent chromatin insulator...
- CTCF-dependent chromatin insulator is linked to epigenetic remodelingKo Ishihara
Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
Mol Cell 23:733-42. 2006..Here, we show that the SNF2-like chromodomain helicase protein CHD8 interacts with the insulator binding protein CTCF. Chromatin immunoprecipitation analysis revealed that CHD8 was present at known CTCF target sites, such as the ..
- CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wideIgor Chernukhin
Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
Mol Cell Biol 27:1631-48. 2007b>CTCF is a transcription factor with highly versatile functions ranging from gene activation and repression to the regulation of insulator function and imprinting...
- Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switchMary E Donohoe
Howard Hughes Medical Institute, USA
Mol Cell 25:43-56. 2007..Although no proven trans-acting factors have been identified, a likely candidate is Ctcf, a chromatin insulator with essential function in autosomal imprinting...
- Analysis of the vertebrate insulator protein CTCF-binding sites in the human genomeTae Hoon Kim
Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA 92093 0653, USA
Cell 128:1231-45. 2007..In vertebrates, insulator's function requires association with the CCCTC-binding factor (CTCF), a protein that recognizes long and diverse nucleotide sequences...
- Functional role of CCCTC binding factor (CTCF) in stress-induced apoptosisTie Li
Department of Medicine, HMC, David Geffen School of Medicine, University of California Los Angeles, Torrance, CA 90502, USA
Exp Cell Res 313:3057-65. 2007b>CTCF, a nuclear transcriptional factor, is a multifunctional protein and involves regulation of growth factor- and cytokine-induced cell proliferation/differentiation...
- Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted lociMario Renda
Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy
J Biol Chem 282:33336-45. 2007The DNA-binding protein CTCF (CCCTC binding factor) mediates enhancer blocking insulation at sites throughout the genome and plays an important role in regulating allele-specific expression at the Igf2/H19 locus and at other imprinted ..
- Functional role of CCCTC binding factor in insulin-stimulated cell proliferationJ Gao
Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, CA 90502, USA
Cell Prolif 40:795-808. 2007CCCTC binding factor (CTCF) is a nuclear protein containing an 11-zinc-finger DNA-binding domain. CTCF plays important roles in the regulation of epigenetics and gene transcription...
- Cohesins localize with CTCF at the KSHV latency control region and at cellular c-myc and H19/Igf2 insulatorsWilliam Stedman
Gene Regulation Program, The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
EMBO J 27:654-66. 2008Cohesins, which mediate sister chromatin cohesion, and CTCF, which functions at chromatin boundaries, play key roles in the structural and functional organization of chromosomes...
- Cohesin mediates transcriptional insulation by CCCTC-binding factorKerstin S Wendt
Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
Nature 451:796-801. 2008..cohesin-binding sites in the human genome and show that most of these are associated with the CCCTC-binding factor (CTCF), a zinc-finger protein required for transcriptional insulation...
- Cohesins functionally associate with CTCF on mammalian chromosome armsVania Parelho
Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
Cell 132:422-33. 2008..occupy a subset of DNase I hypersensitive sites, many of which contain sequence motifs resembling the consensus for CTCF, a DNA-binding protein with enhancer blocking function and boundary-element activity...
- The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactionsParimal Majumder
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
J Exp Med 205:785-98. 2008Knockdown of the insulator factor CCCTC binding factor (CTCF), which binds XL9, an intergenic element located between HLA-DRB1 and HLA-DQA1, was found to diminish expression of these genes...
- CTCF physically links cohesin to chromatinEric D Rubio
Department of Radiation Oncology, University of Washington School of Medicine, Seattle WA 98195, USA
Proc Natl Acad Sci U S A 105:8309-14. 2008..proteomics approach, we show that the STAG1 (Scc3/SA1) subunit of cohesin interacts with the CCTC-binding factor CTCF bound to the c-myc insulator element...
- The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genomeYutao Fu
Bioinformatics Program, Boston University, Boston, Massachusetts, USA
PLoS Genet 4:e1000138. 2008..and we discovered an array of well-positioned nucleosomes flanking sites occupied by the insulator binding protein CTCF across the human genome. These nucleosomes are highly enriched for the histone variant H2A...
- CTCF regulates allelic expression of Igf2 by orchestrating a promoter-polycomb repressive complex 2 intrachromosomal loopTao Li
Medical Service, VA Palo Alto Health Care System, Palo Alto, California 94304, USA
Mol Cell Biol 28:6473-82. 2008b>CTCF is a zinc finger DNA-binding protein that regulates the epigenetic states of numerous target genes...
- Global analysis of the insulator binding protein CTCF in chromatin barrier regions reveals demarcation of active and repressive domainsSuresh Cuddapah
Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genome Res 19:24-32. 2009..They can be functionally classified as either enhancer blockers or domain barriers. CTCF (CCCTC-binding factor) is the only known major insulator-binding protein in the vertebrates and has been shown to ..
- Cell cycle control of Kaposi's sarcoma-associated herpesvirus latency transcription by CTCF-cohesin interactionsHyojeung Kang
The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
J Virol 83:6199-210. 2009..studies have shown that this major latency control region is occupied by the cellular chromatin boundary factor CTCF and chromosome structural maintenance proteins SMC1, SMC3, and RAD21, which comprise the cohesin complex...
- Epigenetic silencing of the p16(INK4a) tumor suppressor is associated with loss of CTCF binding and a chromatin boundaryMichael Witcher
Regulatory Biology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
Mol Cell 34:271-84. 2009..We show that the multifunctional protein CTCF associates in the vicinity of this boundary and absence of binding strongly coincides with p16 silencing in ..
- Targeted deletion of multiple CTCF-binding elements in the human C-MYC gene reveals a requirement for CTCF in C-MYC expressionWendy M Gombert
Black Lowe and Graham PLLC, Seattle, WA, USA
PLoS ONE 4:e6109. 2009..Previous studies examined the functional mechanism of the MYC insulator element MINE and its CTCF binding sites in the context of transgenes that were randomly inserted into the genome by transfection...
- Epigenetic silencing in Friedreich ataxia is associated with depletion of CTCF (CCCTC-binding factor) and antisense transcriptionIrene De Biase
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America
PLoS ONE 4:e7914. 2009....
- Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ationDawn Farrar
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
Mol Cell Biol 30:1199-216. 2010Poly(ADP-ribosyl)ation of the conserved multifunctional transcription factor CTCF was previously identified as important to maintain CTCF insulator and chromatin barrier functions...
- CTCF is a DNA methylation-sensitive positive regulator of the INK/ARF locusCarmen Rodriguez
Institut de Recherche en Cancérologie de Montpellier INSERM Université de Montpellier I U896, CRLC Val d Aurelle Paul Lamarque, 34298 Montpellier Cedex 5, France
Biochem Biophys Res Commun 392:129-34. 2010..The resulting divergent promoter region is bound by the chromatin insulator protein CTCF in association with histone H3 tri-methylated on lysine 4, irrespective of transcription of ANRIL and ARF...
- Epigenetic regulation of the human p53 gene promoter by the CTCF transcription factor in transformed cell linesE Soto-Reyes
Instituto de Fisiologia Celular, Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Mexico D F, Mexico
Oncogene 29:2217-27. 2010..We characterized a binding site for the CCCTC-binding factor (CTCF) in the human p53 gene promoter that contributes to its transcriptional expression, and has the ability to maintain ..
- NF-kappaB subtypes regulate CCCTC binding factor affecting corneal epithelial cell fateLuo Lu
Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, California 90502, USA
J Biol Chem 285:9373-82. 2010CCCTC binding factor (CTCF) controls DNA imprinting, insulates important gene expression, and mediates growth factor- and stress-induced cell fate. However, regulatory mechanisms involved in intracellular CTCF activity are largely unknown...
- Cell type specificity of chromatin organization mediated by CTCF and cohesinChunHui Hou
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:3651-6. 2010b>CTCF sites are abundant in the genomes of diverse species but their function is enigmatic...
- Gene-specific repression of the p53 target gene PUMA via intragenic CTCF-Cohesin bindingNathan P Gomes
Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Genes Dev 24:1022-34. 2010..Interestingly, the insulator protein CTCF (CCCTC-binding factor) and the Cohesin complex occupy these intragenic chromatin boundaries...
- CCCTC-binding factor acts upstream of FOXA1 and demarcates the genomic response to estrogenYu Zhang
Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Peking University Health Science Center, Beijing 100191, China
J Biol Chem 285:28604-13. 2010..boundary elements surrounding the well defined ERalpha target TFF1 locus are occupied by the CCCTC-binding factor (CTCF)...
- Distinct epigenetic domains separated by a CTCF bound insulator between the tandem genes, BLU and RASSF1AJer Wei Chang
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
PLoS ONE 5:e12847. 2010..3. We hypothesized that there may be a concordance on their gene expression and promoter methylation status. If not, then there may be an insulator located between RASSF1A and BLU genes that provides a barrier activity...
- CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancerFrancesco Paolo Fiorentino
Sbarro Health Research Organization, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
Mol Cancer Res 9:225-33. 2011..fibroblasts the expression of Rb2/p130 gene is directly controlled by the chromatin insulator CCCTC-binding factor, CTCF, which by binding to the Rb2/p130 gene promoter induces, and/or maintains, a specific local chromatin organization ..
- Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activityTiaojiang Xiao
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell Biol 31:2174-83. 2011Recent studies have shown that the protein CTCF, which plays an important role in insulation and in large-scale organization of chromatin within the eukaryotic nucleus, depends for both activities on recruitment of the cohesin complex...
- Interruption of intrachromosomal looping by CCCTC binding factor decoy proteins abrogates genomic imprinting of human insulin-like growth factor IIHe Zhang
Department of Biochemistry and Molecular Biology, Ninth People s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
J Cell Biol 193:475-87. 2011Monoallelic expression of IGF2 is regulated by CCCTC binding factor (CTCF) binding to the imprinting control region (ICR) on the maternal allele, with subsequent formation of an intrachromosomal loop to the promoter region...
- Occupancy of chromatin organizers in the Epstein-Barr virus genomeMeghan M Holdorf
Department of Cellular and Molecular Pharmacology, University of California San Francisco, CA 94158, USA
Virology 415:1-5. 2011The human CCCTC-binding factor, CTCF, regulates transcription of the double-stranded DNA genomes of herpesviruses. The architectural complex cohesin and RNA Polymerase II also contribute to this organization...
- Genome-wide studies of CCCTC-binding factor (CTCF) and cohesin provide insight into chromatin structure and regulationBum Kyu Lee
Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas, Austin, Texas 78712, USA
J Biol Chem 287:30906-13. 2012..CCCTC-binding factor (CTCF), a sequence-specific transcription factor, serves as a chromatin organizer in building this complex chromatin ..
- CTCF/cohesin-mediated DNA looping is required for protocadherin α promoter choiceYa Guo
Key Laboratory of Systems Biomedicine, Ministry of Education, Center for Comparative Biomedicine, Institute of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China
Proc Natl Acad Sci U S A 109:21081-6. 2012..In addition, we show that this DNA looping requires specific binding of the CTCF/cohesin complex to two symmetrically aligned binding sites in both the transcriptionally active promoters and in ..
- CTCF mediates the TERT enhancer-promoter interactions in lung cancer cells: identification of a novel enhancer region involved in the regulation of TERT geneVegard Eldholm
Department of Chemical and Biological Work Environment, National Institute of Occupational Health, Oslo, Norway
Int J Cancer 134:2305-13. 2014..TERT is embedded in a region of condensed chromatin and the chromatin remodeling protein CCCTC-binding factor (CTCF) has been implicated in the inhibition of TERT expression...
- Cohesin and CTCF differentially affect chromatin architecture and gene expression in human cellsJessica Zuin
Department of Cell Biology, Biophysical Genomics, Department of Cell Biology, Center for Biomics, Cancer Genomics Center, Erasmus Medical Center, 3015 GE, Rotterdam, The Netherlands
Proc Natl Acad Sci U S A 111:996-1001. 2014..The boundary sequences between domains are enriched for binding sites of CTCC-binding factor (CTCF) and the cohesin complex, implicating these two factors in the establishment or maintenance of topological domains...
- A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancersG N Filippova
Molecular Medicine Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genes Chromosomes Cancer 22:26-36. 1998..We have cloned one such MYC regulatory factor, termed CTCF, which is a highly evolutionarily conserved-11-zinc finger transcriptional factor possessing multiple DNA sequence ..
- The c-myc insulator element and matrix attachment regions define the c-myc chromosomal domainWendy M Gombert
Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98104, USA
Mol Cell Biol 23:9338-48. 2003..The enhancer-blocking activity is mediated by CTCF. Chromatin immunoprecipitation assays demonstrate that CTCF is constitutively bound at the insulator and at the ..
- Epigenetic boundaries of tumour suppressor gene promoters: the CTCF connection and its role in carcinogenesisFelix Recillas-Targa
Instituto de Fisiologia Celular, Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Mexico
J Cell Mol Med 10:554-68. 2006..We describe a previously unsuspected epigenetic factor and propose the incorporation of the 11-zinc finger CCCTC-binding factor, known as CTCF as a novel and multifunctional epigenetic regulator.
- Boundaries between chromosomal domains of X inactivation and escape bind CTCF and lack CpG methylation during early developmentGalina N Filippova
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Dev Cell 8:31-42. 2005..We report that the 5' end of Jarid1c, a mouse escape gene adjacent to an inactivated gene, binds CTCF, displays high levels of histone H3 acetylation, and functions as a CTCF-dependent chromatin insulator...
- Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq dataRaja Jothi
Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA
Nucleic Acids Res 36:5221-31. 2008..by applying it on ChIP-Seq data for three widely studied and well-characterized human transcription factors: CTCF (CCCTC-binding factor), NRSF (neuron-restrictive silencer factor) and STAT1 (signal transducer and activator of ..
- Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor developmentIn Young Park
Department of Biological Sciences, 373 1 Gusung Dong, Korea Advanced Institute of Science and Technology, Yusung Gu, Taejon 305 701, Republic of Korea
J Cell Biochem 94:585-96. 2005..The region containing two CCCTC binding factor (CTCF) binding sites in the 5'-ICR tended to be hypomethylated in the thymus of p53(-/-) mice, but not in the liver...
- Changes in the DNA methylation profile of the rat H19 gene upstream region during development and transgenic hepatocarcinogenesis and its role in the imprinted transcriptional regulation of the H19 geneHerbert Manoharan
McArdle Laboratory for Cancer Research, Medical School, University of Wisconsin, Madison, Wisconsin 53706 1599, USA
Mol Carcinog 41:1-16. 2004..Similar to mouse and human, we showed that the zinc-finger CCTCC binding factor (CTCF) binds to the unmethylated CTCF binding site in the upstream region to influence monoallelic imprinted expression ..
- CTCFL/BORIS is a methylation-independent DNA-binding protein that preferentially binds to the paternal H19 differentially methylated regionPhuongmai Nguyen
Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
Cancer Res 68:5546-51. 2008The CTCF paralog BORIS (brother of the regulator of imprinted sites) is an insulator DNA-binding protein thought to play a role in chromatin organization and gene expression...
- Enhancer blocking by chicken beta-globin 5'-HS4: role of enhancer strength and insulator nucleosome depletionHui Zhao
Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
J Biol Chem 281:30573-80. 2006..Nucleosome depletion at 5'-HS4 was dependent on interaction of the insulator protein CCCTC-binding factor (CTCF) and was required for enhancer blocking...
- Dynamics of alpha-globin locus chromatin structure and gene expression during erythroid differentiation of human CD34(+) cells in cultureMilind C Mahajan
Department of Genetics, The Anlyan Center, Yale University School of Medicine, New Haven, CT 06510, USA
Exp Hematol 37:1143-1156.e3. 2009....
- CTCF binding site classes exhibit distinct evolutionary, genomic, epigenomic and transcriptomic featuresKobby Essien
Penn Center for Bioinformatics, Department of Genetics, 415 Curie Boulevard, University of Pennsylvania, Philadelphia, PA 19104, USA
Genome Biol 10:R131. 2009b>CTCF (CCCTC-binding factor) is an evolutionarily conserved zinc finger protein involved in diverse functions ranging from negative regulation of MYC, to chromatin insulation of the beta-globin gene cluster, to imprinting of the Igf2 locus...
- Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein geneW W Quitschke
Department of Psychiatry and Behavioral Science, State University of New York at Stony Brook, Stony Brook, NY 11794 8101, USA
Nucleic Acids Res 28:3370-8. 2000High levels of transcription from the amyloid precursor protein promoter are dependent on the binding of CTCF to the APBbeta core recognition sequence located between positions -82 and -93 upstream from the transcriptional start site...
- Systematic discovery of regulatory motifs in conserved regions of the human genome, including thousands of CTCF insulator sitesXiaohui Xie
Broad Institute of MIT and Harvard, Massachusetts Institute of Technology and Harvard Medical School, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 104:7145-50. 2007..We demonstrate by biochemical and computational methods that it defines a binding site for the CTCF protein, which is involved in insulator function to limit the spread of gene activation...
- CTCF-independent, but not CTCF-dependent, elements significantly contribute to TCR-alpha locus control region activityJanette Gomos-Klein
Department of Biological Sciences, City University of New York, Hunter College, New York, NY 10021, USA
J Immunol 179:1088-95. 2007..Most vertebrate enhancer-blocking insulators are dependent on the CTCF transcription factor and its cognate DNA binding site...
- MECP2 promoter methylation and X chromosome inactivation in autismRaman P Nagarajan
Medical Microbiology and Immunology and Rowe Program in Human Genetics, University of California, Davis, CA 95616, USA
Autism Res 1:169-78. 2008..Chromatin immunoprecipitation analysis demonstrated that the CCTC-binding factor (CTCF) bound to this transition region in neuronal cells, consistent with a chromatin boundary at the methylation ..
- Hierarchical hidden Markov model with application to joint analysis of ChIP-chip and ChIP-seq dataHyungwon Choi
Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
Bioinformatics 25:1715-21. 2009..Therefore, it is of interest to see whether the two data sources can be combined to enhance the detection of TFBS...
- CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple formsE M Klenova
Institute of Carcinogenesis, Russian Cancer Research Center, Moscow
Mol Cell Biol 13:7612-24. 1993A novel sequence-specific DNA-binding protein, CTCF, which interacts with the chicken c-myc gene promoter, has been identified and partially characterized (V. V. Lobanenkov, R. H. Nicolas, V. V. Adler, H. Paterson, E. M. Klenova, A. V...
- Nucleosome positioning signals in the DNA sequence of the human and mouse H19 imprinting control regionsC Davey
Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King s Buildings, West Mains Road, Scotland EH9 3JR, Edinburgh, UK
J Mol Biol 325:873-87. 2003..to flank and encompass the short conserved sequences that constitute the binding sites for the zinc finger protein CTCF, an essential mediator of insulator activity...
- Modular insulators: genome wide search for composite CTCF/thyroid hormone receptor binding sitesOliver Weth
Institute for Genetics, Justus Liebig University Giessen, Giessen, Germany
PLoS ONE 5:e10119. 2010The conserved 11 zinc-finger protein CTCF is involved in several transcriptional mechanisms, including insulation and enhancer blocking...
- The pluripotency factor Oct4 interacts with Ctcf and also controls X-chromosome pairing and countingMary E Donohoe
Howard Hughes Medical Institute, USA
Nature 460:128-32. 2009..Xite, two regulatory noncoding RNA genes of the X-inactivation centre, and also complexes with XCI trans-factors, Ctcf and Yy1 (ref. 17), through protein-protein interactions...
- Analysis of the IGF2/H19 imprinting control region uncovers new genetic defects, including mutations of OCT-binding sequences, in patients with 11p15 fetal growth disordersJulie Demars
Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, Melbourne, 3004 VIC, Australia
Hum Mol Genet 19:803-14. 2010..5. This methylation-sensitive chromatin insulator works by binding the zinc-finger protein CTCF in a parent-specific manner...
- Disruption of genomic neighbourhood at the imprinted IGF2-H19 locus in Beckwith-Wiedemann syndrome and Silver-Russell syndromeRaffaella Nativio
Department of Oncology, Cancer Research UK Cambridge Research Institute, University of Cambridge, LiKaShing Centre, Cambridge, UK
Hum Mol Genet 20:1363-74. 2011..of the ICR controls the reciprocal imprinting of IGF2 and H19 by preventing the binding of the insulator protein, CTCF. We here show that local changes in histone modifications and CTCF--cohesin binding at the ICR in BWS and SRS ..
- Regulatory factor interactions and somatic silencing of the germ cell-specific ALF geneMinjung Kim
Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas 75080, USA
J Biol Chem 281:34288-98. 2006..Two additional complexes (C1 and C3) are due to the multivalent zinc finger protein CTCF, a factor that plays a role in gene silencing and chromatin insulation...
- Chromatin profiling of Epstein-Barr virus latency control regionLatasha Day
The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
J Virol 81:6389-401. 2007..binding patterns of EBNA1 with those of the origin recognition complex protein ORC2, the chromatin boundary factor CTCF, the linker histone H1, and several histone modifications...
- Duplication of the paternal IGF2 allele in trisomy 11 and elevated expression levels of IGF2 mRNA in congenital mesoblastic nephroma of the cellular or mixed typeNaoki Watanabe
Department of Cancer Diagnosis, Research Institute for Clinical Oncology, Saitama Cancer Center, Ina, Saitama, and Department of Pediatrics, Juntendo University, Nerima Hospital, Tokyo, Japan
Genes Chromosomes Cancer 46:929-35. 2007..with disomy 11 showed one paternal and one maternal allele of IGF2, analyzing the methylation status of the sixth CTCF site of the H19-differentially methylated region...
- Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosisJun ichiro Komura
Department of Cell Biology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980 8575, Japan
Proc Natl Acad Sci U S A 104:15741-6. 2007..Recently, it has been reported that CTCF, a major protein in vertebrate insulator elements, remains associated with mitotic chromatin...
- Evolutionary rate heterogeneity of Alu repeats upstream of the APOA5 gene: do they regulate APOA5 expression?Edward A Ruiz-Narváez
Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
J Hum Genet 53:247-53. 2008..Also, we scanned the intergenic region for the presence of binding sites of the insulator protein CTCF. Seven out of the nine found CTCF binding sites were located in the first half of the intergenic region...
- Positive regulation of c-Myc by cohesin is direct, and evolutionarily conservedJenny M Rhodes
Department of Pathology, Dunedin School of Medicine, The University of Otago, Dunedin, New Zealand
Dev Biol 344:637-49. 2010..Roles for cohesin in regulating gene expression, sometimes in combination with CCCTC-binding factor (CTCF), have emerged...
- A DNA insulator prevents repression of a targeted X-linked transgene but not its random or imprinted X inactivationDominic Ciavatta
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 103:9958-63. 2006..Thus, the chicken hypersensitive site 4 DNA insulator is sufficient to protect an X-linked gene from repression during development but not from X inactivation...
- IGF2/H19 hypomethylation in Silver-Russell syndrome and isolated hemihypoplasiaMichael Zeschnigk
Institut fur Humangenetik, Universitatsklinikum Essen, Essen, Germany
Eur J Hum Genet 16:328-34. 2008..found to be hypomethylated, as determined by Southern blot analysis of an HpaII restriction site close to the third CTCF-binding site (CTS3) within ICR1...
- Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermiaC J Marques
Department of Genetics, Faculty of Medicine, Porto 4200 319, Portugal
Mol Hum Reprod 14:67-74. 2008..54% (1.2-8.3%) and complete unmethylation in 2.95% (0-5.9%) of H19 clones. Similarly, for the CTCF-binding site 6, hypomethylation occurred in 4.8% (1.2-8.9%) and complete unmethylation in 3.7% (0-6...
- Epigenetic regulation of latent HSV-1 gene expressionDavid C Bloom
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610 0266, USA
Biochim Biophys Acta 1799:246-56. 2010..Binding of CTCF, a protein that binds to all known vertebrate insulators, to sites within the HSV-1 genome likely prevents ..
- Epigenetic control of expression of the human L- and M- pigment genesS S Deeb
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA, USA
Ophthalmic Physiol Opt 30:446-53. 2010..This DHS region contains a highly conserved motif that binds CCCTC-binding factor (CTCF), referred to as a 'chromatin insulator or boundary element', that has been shown to regulate gene expression at ..
- Epigenetic regulation of latent Epstein-Barr virus promotersMaria Takacs
Division of Virology, National Center for Epidemiology, H 1097 Budapest, Gyali ut 2 6, Hungary
Biochim Biophys Acta 1799:228-35. 2010..We speculate that binding of the chromatin insulator protein CTCF to 3 distinct sites (within, close to and far from the matrix attachment region) may contribute to the three-..
- Loss of imprinting and cancerP Jelinic
Division of Experimental Pathology, University Institute of Pathology, Lausanne, Switzerland
J Pathol 211:261-8. 2007..Although the major reading mechanisms of imprinted status are known, chromatin boundary formation by CTCF and untranslated RNAs, the molecules elaborating the initial ICR methylation, are just being uncovered...
- The CTCF insulator protein is posttranslationally modified by SUMOMelissa J MacPherson
Department of Molecular Genetics, 1 King s College Circle, University of Toronto, Toronto, ON, Canada
Mol Cell Biol 29:714-25. 2009The CTCF protein is a highly conserved zinc finger protein that is implicated in many aspects of gene regulation and nuclear organization. Its functions include the ability to act as a repressor of genes, including the c-myc oncogene...
- Negative protein 1, which is required for function of the chicken lysozyme gene silencer in conjunction with hormone receptors, is identical to the multivalent zinc finger repressor CTCFM Burcin
, Giessen, Germany
Mol Cell Biol 17:1281-8. 1997..Another protein, CCCTC-binding factor (CTCF), specifically binds to 50-bp-long sequences that contain repetitive CCCTC elements in the vicinity of vertebrate c-..
- Jean Pierre J Issa; Fiscal Year: 2016..to DNA methylation (the transcription factor Sp1, LINE/SINE retrotransposons and the insulator proteins CTCF, USF1/2 and VEZF1) will be tested in specific aim 2...
- SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016..genes by recruiting multifunctional transcription factor Early Growth Response gene (Egr-1) and chromatin insulator CTCF to the key regulatory regions of HSV-1...
- Ann Harris; Fiscal Year: 2015..that likely establish the looped conformation of the active locus and determine how trans-acting factors such as CTCF and cohesin influence this spatial organization...
- Epigenetic Regulation of Serotonin:Relevance to HIV and Methamphetamine AbuseGREGORY MICHAEL MILLER; Fiscal Year: 2012..We will also assess the involvement of DNA methylation and CCCTC-binding factor (CTCF) in the regulation of TPH2 gene expression...
- Epigenetic Regulation of Imprinting in Mouse EmbryoMarisa S Bartolomei; Fiscal Year: 2012..Specific Aim 1 will examine the general role of the multi-functional protein CTCF, which provides enhancer blocking function and a DNA methylation-free region on the imprinting control region (ICR) ..
- Paul M Lieberman; Fiscal Year: 2016..protein Rb (aim1), the viral- encoded origin binding protein EBNA1 (aim 2), and the chromatin boundary factor CTCF (aim 3)...
- Marisa S Bartolomei; Fiscal Year: 2016..The ICR, which is also designated the differentially methylated domain (DMD), acts as a methylation-senstitive, CTCF-dependent insulator...
- Tae Hoon Kim; Fiscal Year: 2014..We have found that the insulator protein CTCF binding sites define precise molecular boundaries at a number of large heterochromatin domains found in the human ..
- EPIGENETIC REGULATION IN A CANCER ASSOCIATED REGIONMichael J Higgins; Fiscal Year: 2012..Moreover, we have shown that the epigenetic regulatory protein CTCF binds to the minimal repressive sequence in an allele-specific manner...
- Jeremy M Boss; Fiscal Year: 2015..MHC-II gene transcription and these interactions are dependent on the insulator-binding factor CTCF. Our observations establish a new paradigm for how these genes are regulated...
- Role of CTCF in V(D)J RearrangementSTEPHANIE CHRISTINE DEGNER; Fiscal Year: 2010..The nuclear factors controlling Ig loci contraction are not known. CTCF (CCCTC-binding factor) is a nuclear protein that participates in long-range chromosomal interactions...
- Library Screening for Novel Enhancer Blockers Derived from Human T LymphocytesBrian P Sorrentino; Fiscal Year: 2013..Jude for homologous gene targeting in Jurkat T cells to screen a library of CTCF binding sequences derived from T cells for enhancer blocking activity...
- Paul M Lieberman; Fiscal Year: 2015..that this region of the viral chromosome is protected from epigenetic silencing by the chromatin insulator protein CTCF and colocalization with cohesin subunits (e.g. SMC1, SMC3, Rad21)...
- BEVERLY MARIE EMERSON; Fiscal Year: 2015..We find that loss of CTCF-dependent chromatin boundaries, which protects genes from adjacent heterochromatin domains, results in ..
- FUNCTION OF THE SUBUNITS OF EUKARYOTIC CHROMOSOMESWILLIAM GARRARD; Fiscal Year: 2013..4. To determine the impact of separately mutating Ikaros and CTCF binding sites within Sis on Ig loci nuclear organization, distal-Sis-interacting sequences, and repertoire.
- Christine M Disteche; Fiscal Year: 2016..We discovered two regions that bind CTCF only on the inactive X and produce non-coding RNAs that escape X inactivation...
- Regulation of HSV-1 gene expression and reactivation by insulator protein CTCFDonna M Neumann; Fiscal Year: 2013..We have also identified clusters of DNA binding motifs for the cellular insulating protein, CTCF around genomic regions of HSV-1 that are critical for both the establishment of latency as well as in reactivation...
- Heterochromatin on the human inactive X chromosomeBrian P Chadwick; Fiscal Year: 2012..Intriguingly, the chromatin insulator and epigenetic organizer protein CTCF binds specifically to the Xi array, immediately adjacent to the DXZ4 bi-directional promoter...
- Duplin: A Novel A-Kinase Anchoring ProteinMAUREEN E O apos DONNELL; Fiscal Year: 2010..in the embryonic mouse, and is required for chromatin insulation in conjunction with the transcription represser CTCF. Duplin/Chd8 has been characterized as a developmental protein, but is also expressed adult tissue...
- Elliott D Kieff; Fiscal Year: 2016..also co-activated genome wide by EBNALP, which binds mostly to promoters, which are marked by the presence of YY1, CTCF, and ZNF143, as well as Histone H2-Az...
- Mary E Donohoe; Fiscal Year: 2014..The role of the chromatin insulator Ctcf and its protein partners will be investigated in the transcriptional regulation of MeCP2...
- Nuclear organization in stem and differentiated cellsVictor G Corces; Fiscal Year: 2010..Preliminary results in our lab suggest that the organization of CTCF insulators in mouse stem cells changes as these cells differentiate...
- Joaquin M Espinosa; Fiscal Year: 2016..Each of these mechanisms will be further investigated by developing the following Specific Aims: 1. To define how CTCF, Polycomb complexes and lncRNAs regulate PUMA...
- Regulation of EBV Latency by Chromosome ConformationItalo Tempera; Fiscal Year: 2012..Moreover, we found that the cellular factor CTCF binds at several key regions of the EBV genome and regulates promoter selection and viral gene expression...
- CCCTC-binding factor (CTCF) in trinucleotide repeat instability and diseaseAlbert R La Spada; Fiscal Year: 2010..Within this 3'region is a binding site for the "CCCTC-binding factor" (CTCF), a protein with a variety of functions that stem from its ability to modulate DNA structure...
- Aaron F Straight; Fiscal Year: 2016..new methods to measure the formation of looped domains dependent upon the activity of the chromatin protein CTCF. CTCF is known to be required for the stabilization of looped regions in the genome but how it generates or ..
- MARTIN JOHN WALSH; Fiscal Year: 2014..First, we will investigate the role of CHD6 to cooperate with additional transcriptional proteins such as CTCF to regulate CFTR...
- Next generation sequence analysis of the IgH repertoireAnn J Feeney; Fiscal Year: 2012..Therefore, we will compare rearrangement frequencies to the locations of CTCF/cohesin sites...
- MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATEDavid F Jarrard; Fiscal Year: 2013..of IGF2 LOI on prostate carcinogenesis in a susceptible mouse, ii) determining whether interventions that induce CTCF maintain IGF2 imprinting, and iii) define and validate IGF2 LOI in the `field defect'associated with human ..
- Italo Tempera; Fiscal Year: 2014..Moreover, we found that the cellular factor CTCF binds at several key regions of the EBV genome and regulates promoter selection and viral gene expression...
- Michael S Krangel; Fiscal Year: 2015..manipulated strains of mice will be used to test the roles of TCR locus enhancers and the roles of proteins CTCF and cohesin in defining locus conformational states...
- Ann J Feeney; Fiscal Year: 2014..This is accomplished by contraction of the Vh locus via multiple chromosomal looping events. CTCF is a zinc finger protein that is associated with insulators and boundary elements at several well-studied ..
- John A Stamatoyannopoulos; Fiscal Year: 2016..therein, and to continue to provide relevant synergistic annotations including RNA-seq, histone modifications, and CTCF, as well as DNA methylation. The overriding focus of our production effort has been on data quality...
- Stephen J Tapscott; Fiscal Year: 2016..The aims of Project 3 (Dr. Filippova) will be to determine whether CTCF binding on the disease associated allele regulates regional chromatin structure, RNA transcription, or nuclear ..
- CTCF peptides - a new therapeutic alternative to target HIV in the brain.Malgorzata Simm; Fiscal Year: 2012..Recently we identified the HRF as a truncated form of a potent transcription suppressor - CTCF (tCTCF)...
- Frederick W Alt; Fiscal Year: 2015..We have found that the 5'D4KBS functionally employs a CTCF looping/insulator factor binding site, at least for its lineage-specific function...
- Carl L Schildkraut; Fiscal Year: 2016..We will also determine the contribution of CTCF binding to FMR1 replication programs...
- VISUAL NEURONS--DEVELOPMENT AND REGENERATION IN VITROMARIA VALERIA CANTO SOLER; Fiscal Year: 2010..Functional analysis will focus on CTCF, a multifunctional member of the Zn-finger family of transcriptional regulators, which is hypothesized to act as a ..
- MARIA VALERIA CANTO-SOLER; Fiscal Year: 2016..Within this framework, we have identified CTCF, a multifunctional transcriptional regulator previously unknown to participate in these phenomena, as a leading ..
- Role of regulatory sequence at the H19 imprinting control regionFolami Ideraabdullah; Fiscal Year: 2009..Previous studies have shown that binding sites for the insulator protein CTCF present at the ICR are necessary for imprinting control...
- The Role of CTG Repeats in DM1 Locus RegulationSARA GEORGES; Fiscal Year: 2006..My central hypothesis is that the precise establishment of chromatin structure by wild type CTG repeats and CTCF binding sites at the DM1 insulator is critical for the maintenance of transcriptional regulation at the DM1 locus, ..
- STUDY ON A NOVEL ALZHEIMER APP REGULATORY FACTORWolfgang Quitschke; Fiscal Year: 2006..This element binds the multivalent and multifunctional transcription factor CTCF, which acts as a transcriptional activator...
- Analysis of transcription in lens using tiled microarrays (ChIP on chip)Ales Cvekl; Fiscal Year: 2007..focusing on histone H3 K9 acetylation, H3 K3/27 methylation, methylation of DMA, binding of insulator protein CTCF and chromatin activator HMGN3, all involved in epigenetic regulation...
- Evolutionary analysis of CTCF and potential links to breast cancer phenotypesChristopher Carlson; Fiscal Year: 2007..One region with frequent loss of heterozygosity in breast cancer is chromosome 16q22 , within which the CTCF multifunctional transcription factor lies, and CTCF expression in breast cancer cells has been associated with ..
- Genetic and Epigenetic Markers in Ovarian CancerElizabeth Swisher; Fiscal Year: 2006..In ovarian cancers, evaluate expression of BRCA1 and other proteins that may interact with BRCA1 including Id4, CTCF, and ATM, correlating protein expression with clinical outcomes in both hereditary and sporadic ovarian cancer...
- Epigenetic effects if endocrine disruptors in fetal germ cellsPIROSKA E SZABO; Fiscal Year: 2010....
- 2006 Mammalian Gametogenesis/Embryogenesis GordonMarisa Bartolomei; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Genetically Modified Rhesus MonkeysShoukhrat Mitalipov; Fiscal Year: 2006..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
- Quantitating chromatin dynamics during erythropoiesisM Bender; Fiscal Year: 2006..unreadable] [unreadable]..
- Mouse Models of Tumor Progression and Therapy ResponseCHRISTOPHER KEMP; Fiscal Year: 2007..These experiments will reveal the molecular and cellular basis for the poor clinical outcome associated with p27 deficient tumors. This knowledge can then be applied to improve treatment design and regimen in the clinical setting. ..
- Chromatin Insulator Function at the C-myc GeneAnton Krumm; Fiscal Year: 2007..The results will broaden our understanding of the role of chromatin elements in the specific regulation and nuclear organization of mammalian oncogenes in normal and malignant cells. ..
- MOLECULAR GENETICS OF COLOR VISIONSamir Deeb; Fiscal Year: 2008..This will also help in developing cell-specific gene therapy strategies. ..
- Maternal-Fetal Conflict: The Effect of Imprinted Genes on Fetal GrowthRonald M Adkins; Fiscal Year: 2010..Dietary survey instruments during pregnancy and just afterwards will be used to determine the maternal intake of nutrients vital to DNA methylation. Statistical analyses will be performed to test the three hypotheses. ..
- The Role of Double Strand Breaks in CarcinogenesisCHRISTOPHER KEMP; Fiscal Year: 2006..Understanding the functional interaction of the P13 Ks, in regulating p53, apoptosis, development and carcinogenesis at the level of the whole animal is a necessary link to apply knowledge gained from cell culture models to the clinic. ..
- FSHD: Chromatin Structure, Looping, & ExpressionMelanie Ehrlich; Fiscal Year: 2009..The proposed research should elucidate new aspects of long-distance control of gene expression as well as lending clinically useful insights into this currently intractable disease. ..
- Genomics and Epigenomics of Fetal Growth RegulationRonald M Adkins; Fiscal Year: 2010..Statistical analyses will be performed to test the three hypotheses. Note: The only change from the original abstract is a change from 1,000 to 800 mothernewborn pairs. ..
- Gene Activation by Remote Transcriptional EnhancersDale Dorsett; Fiscal Year: 2004..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
- EARLY RESPONSE OF CORNEAL EPITHELIUM TO UV-INDUCED DEATHLuo Lu; Fiscal Year: 2004..Furthermore therapeutic measures may be identified which could reduce the incidence of UV-induced apoptotic corneal epithelial damage and increases in corneal epithelial susceptibility to infection and diseases. ..
- Structure and Function of CTCF: Mouse Model StudiesGalina Filippova; Fiscal Year: 2007DESCRIPTION (provided by applicant): CTCF is a highly conserved 11 Zn finger (ZF) transcription factor and insulator protein...
- MOLECULAR GENETICS OF COLOR VISIONSamir S Deeb; Fiscal Year: 2010..Selective Tr2 ligands could potentially be designed to correct abnormal phenotypes during retinal development. ..
- SEQUENCES CONTROLLING H19 GENE IMPRINTINGMarisa S Bartolomei; Fiscal Year: 2010..DMD), is hypermethylated on the repressed paternal allele and acts as a methylation-sensitive insulator through CTCF-binding on the maternal allele...
- T cell activating genePatent Number: WO2004058805-A2; Date:2004-07-15