CTCF

Summary

Gene Symbol: CTCF
Description: CCCTC-binding factor
Alias: MRD21, transcriptional repressor CTCF, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor (zinc finger protein), CTCFL paralog
Species: human

Top Publications

  1. pmc Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2
    Wei Chi Ku
    Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan
    Mol Cell Proteomics 8:2034-50. 2009
  2. ncbi CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:520-7. 2001
  3. pmc CTCF regulates cell cycle progression of alphabeta T cells in the thymus
    Helen Heath
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 27:2839-50. 2008
  4. ncbi A loss of insulin-like growth factor-2 imprinting is modulated by CCCTC-binding factor down-regulation at senescence in human epithelial cells
    Vivian X Fu
    Department of Surgery, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53792, USA
    J Biol Chem 279:52218-26. 2004
  5. ncbi Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter tts DNA-binding specificity
    Galina N Filippova
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cancer Res 62:48-52. 2002
  6. ncbi YB-1 and CTCF differentially regulate the 5-HTT polymorphic intron 2 enhancer which predisposes to a variety of neurological disorders
    Elena Klenova
    Department of Biological Sciences, University of Essex, Essex CO4 3SQ, United Kingdom
    J Neurosci 24:5966-73. 2004
  7. pmc An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes
    G N Filippova
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Mol Cell Biol 16:2802-13. 1996
  8. ncbi The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activation
    A A Vostrov
    Department of Psychiatry and Behavioral Science, State University of New York, Stony Brook, New York 11794 8101, USA
    J Biol Chem 272:33353-9. 1997
  9. ncbi The protein CTCF is required for the enhancer blocking activity of vertebrate insulators
    A C Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Cell 98:387-96. 1999
  10. ncbi An element in the region responsible for premature termination of transcription mediates repression of c-myc gene expression by thyroid hormone in neuroblastoma cells
    G Perez-Juste
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas and Universidad Aut noma de Madrid, 28029 Madrid, Spain
    J Biol Chem 275:1307-14. 2000

Research Grants

  1. Jean Pierre J Issa; Fiscal Year: 2016
  2. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
  3. Ann Harris; Fiscal Year: 2015
  4. Epigenetic Regulation of Serotonin:Relevance to HIV and Methamphetamine Abuse
    GREGORY MICHAEL MILLER; Fiscal Year: 2012
  5. Epigenetic Regulation of Imprinting in Mouse Embryo
    Marisa S Bartolomei; Fiscal Year: 2012
  6. Paul M Lieberman; Fiscal Year: 2016
  7. Marisa S Bartolomei; Fiscal Year: 2016
  8. Tae Hoon Kim; Fiscal Year: 2014
  9. EPIGENETIC REGULATION IN A CANCER ASSOCIATED REGION
    Michael J Higgins; Fiscal Year: 2012
  10. Jeremy M Boss; Fiscal Year: 2015

Patents

  1. T cell activating gene

Detail Information

Publications174 found, 100 shown here

  1. pmc Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2
    Wei Chi Ku
    Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Institute of Chemistry, Academia Sinica, Taipei 115, Taiwan
    Mol Cell Proteomics 8:2034-50. 2009
    ..SNF complex. In addition to previously known functions of AP-4, our data suggest that AP-4 participates in a transcriptional-regulating complex at the HDM2-P2 promoter in response to DNA damage...
  2. ncbi CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease
    R Ohlsson
    Dept of Genetics and Development, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Trends Genet 17:520-7. 2001
    b>CTCF is an evolutionarily conserved zinc finger (ZF) phosphoprotein that binds through combinatorial use of its 11 ZFs to approximately 50 bp target sites that have remarkable sequence variation...
  3. pmc CTCF regulates cell cycle progression of alphabeta T cells in the thymus
    Helen Heath
    Department of Cell Biology and Genetics, Erasmus MC, Rotterdam, The Netherlands
    EMBO J 27:2839-50. 2008
    The 11-zinc finger protein CCCTC-binding factor (CTCF) is a highly conserved protein, involved in imprinting, long-range chromatin interactions and transcription...
  4. ncbi A loss of insulin-like growth factor-2 imprinting is modulated by CCCTC-binding factor down-regulation at senescence in human epithelial cells
    Vivian X Fu
    Department of Surgery, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53792, USA
    J Biol Chem 279:52218-26. 2004
    ..As a mechanism, a 2-fold decrease in the binding of the enhancer-blocking element CCCTC-binding factor (CTCF) within the intergenic IGF2-H19 region was found to underlie this switch to biallelic IGF2 expression in senescent ..
  5. ncbi Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter tts DNA-binding specificity
    Galina N Filippova
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cancer Res 62:48-52. 2002
    b>CTCF is a widely expressed 11-zinc finger (ZF) transcription factor that is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin ..
  6. ncbi YB-1 and CTCF differentially regulate the 5-HTT polymorphic intron 2 enhancer which predisposes to a variety of neurological disorders
    Elena Klenova
    Department of Biological Sciences, University of Essex, Essex CO4 3SQ, United Kingdom
    J Neurosci 24:5966-73. 2004
    ..Interestingly, the transcription factor CCTC-binding factor (CTCF), previously shown to interact with YB-1, interferes with the ability of the VNTR to support YB-1-directed reporter ..
  7. pmc An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes
    G N Filippova
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Mol Cell Biol 16:2802-13. 1996
    We have isolated and analyzed human CTCF cDNA clones and show here that the ubiquitously expressed 11-zinc-finger factor CTCF is an exceptionally highly conserved protein displaying 93% identity between avian and human amino acid ..
  8. ncbi The zinc finger protein CTCF binds to the APBbeta domain of the amyloid beta-protein precursor promoter. Evidence for a role in transcriptional activation
    A A Vostrov
    Department of Psychiatry and Behavioral Science, State University of New York, Stony Brook, New York 11794 8101, USA
    J Biol Chem 272:33353-9. 1997
    ..gel electrophoresis, partial peptide sequences were obtained that completely matched the transcriptional factor CTCF. This protein is a known regulator of c-myc and lysozyme gene expression, and it binds to a variety of diverse DNA ..
  9. ncbi The protein CTCF is required for the enhancer blocking activity of vertebrate insulators
    A C Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Cell 98:387-96. 1999
    ..We show that this sequence is the binding site for CTCF, a previously identified eleven-zinc finger DNA-binding protein that is highly conserved in vertebrates...
  10. ncbi An element in the region responsible for premature termination of transcription mediates repression of c-myc gene expression by thyroid hormone in neuroblastoma cells
    G Perez-Juste
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas and Universidad Aut noma de Madrid, 28029 Madrid, Spain
    J Biol Chem 275:1307-14. 2000
    ..These studies provide insight into mechanisms by which TR mediate transcriptional repression and contribute to the understanding of the important effects of thyroid hormones on growth and differentiation of neuronal cells...
  11. pmc Transcriptional repression by the insulator protein CTCF involves histone deacetylases
    M Lutz
    Genetisches Institut der Justus Liebig Universitat, Heinrich Buff Ring 58 62, 35392 Giessen, Germany
    Nucleic Acids Res 28:1707-13. 2000
    The highly conserved zinc-finger protein, CTCF, is a candidate tumor suppressor protein that binds to highly divergent DNA sequences...
  12. ncbi Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene
    A C Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Nature 405:482-5. 2000
    ..The activity of this element is dependent upon the vertebrate enhancer-blocking protein CTCF. Methylation of CpGs within the CTCF-binding sites eliminates binding of CTCF in vitro, and deletion of these sites ..
  13. ncbi CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus
    A T Hark
    Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    Nature 405:486-9. 2000
    ..Furthermore, we show that CTCF, a zinc finger protein implicated in vertebrate boundary function, binds to several sites in the unmethylated ..
  14. ncbi CTCF, a candidate trans-acting factor for X-inactivation choice
    Wendy Chao
    Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Science 295:345-7. 2002
    ..Here, we identify the insulator and transcription factor, CTCF, as a candidate trans-acting factor for X-chromosome selection...
  15. pmc Multiple nucleosome positioning sites regulate the CTCF-mediated insulator function of the H19 imprinting control region
    Meena Kanduri
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, S 752 36 Uppsala, Sweden
    Mol Cell Biol 22:3339-44. 2002
    ..set the stage for the remarkably precise distribution of the four target sites for the chromatin insulator protein CTCF to nucleosome linker sequences in the H19 ICR...
  16. ncbi Transforming growth factor-beta-induced transcription of the Alzheimer beta-amyloid precursor protein gene involves interaction between the CTCF-complex and Smads
    Teralee Burton
    The Dr John Foerster Centre for Health Research on Aging St Boniface General Hospital Research Centre, 351 Tache Avenue, Winnipeg, MB, Canada R3E 0W3
    Biochem Biophys Res Commun 295:713-23. 2002
    ..This site interacts with the zinc finger nuclear factor CTCF, involved in APP transcriptional activity...
  17. ncbi CTCF maintains differential methylation at the Igf2/H19 locus
    Christopher J Schoenherr
    Department of Cell and Structural Biology, University of Illinois, Urbana Champaign, Urbana, Illinois 61801, USA
    Nat Genet 33:66-9. 2003
    ..One candidate regulator of methylation, the zinc-finger protein CTCF, binds to the imprinting control region (ICR) of the genes Igf2 (encoding insulin-like growth factor 2) and H19 (..
  18. pmc CTCF functions as a critical regulator of cell-cycle arrest and death after ligation of the B cell receptor on immature B cells
    Chen Feng Qi
    Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:633-8. 2003
    ..b>CTCF is a transcription factor identified as a repressor of MYC recently shown to cause cell growth inhibition...
  19. ncbi CTCF gene mutations in invasive ductal breast cancer
    Sebastian Aulmann
    Department of Pathology, University of Heidelberg, Germany
    Breast Cancer Res Treat 80:347-52. 2003
    The CTCF gene encodes for a transcriptional repressor of the c-myc oncogene and has previously been mapped to one of the smallest regions of overlapping interstitial deletions on chromosome 16q22.1 in invasive breast cancer...
  20. pmc Human beta-globin locus control region HS5 contains CTCF- and developmental stage-dependent enhancer-blocking activity in erythroid cells
    Keiji Tanimoto
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tennoudai 1 1 1, Tsukuba, Ibaraki 305 8577, Japan
    Mol Cell Biol 23:8946-52. 2003
    ..describe genetic evidence demonstrating that human HS5 includes enhancer-blocking (insulator) activity that is both CTCF and developmental stage dependent...
  21. ncbi Transgenic RNAi reveals essential function for CTCF in H19 gene imprinting
    Andrew M Fedoriw
    Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 303:238-40. 2004
    ..Insulin-like growth factor 2 expression involves binding of the vertebrate insulator protein, CCCTC binding factor (CTCF), to the maternally hypomethylated differentially methylated domain (DMD)...
  22. ncbi CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species
    Timur M Yusufzai
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 13:291-8. 2004
    ..Almost all described vertebrate insulators require binding of the regulatory protein CTCF for their activity...
  23. ncbi Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation
    Wenqiang Yu
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Nat Genet 36:1105-10. 2004
    ..We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation...
  24. ncbi CTCF regulates growth and erythroid differentiation of human myeloid leukemia cells
    Verónica Torrano
    Grupo de Biología Molecular del Cáncer, Departamento de Biologia Molecular, Unidad de Biomedicina CSIC, Universidad de Cantabria, 39011 Santander, Spain
    J Biol Chem 280:28152-61. 2005
    b>CTCF is a transcription factor and a candidate tumor suppressor that contains a DNA-binding domain composed of 11 zinc fingers...
  25. ncbi Heightened expression of CTCF in breast cancer cells is associated with resistance to apoptosis
    France Docquier
    Department of Biological Sciences, Central Campus, University of Essex, Essex, United Kingdon
    Cancer Res 65:5112-22. 2005
    b>CTCF is a candidate tumor suppressor gene encoding a multifunctional transcription factor...
  26. pmc CTCF binding and higher order chromatin structure of the H19 locus are maintained in mitotic chromatin
    Les J Burke
    Institute for Genetics, Justus Liebig Universitaet Giessen, Giessen, Germany
    EMBO J 24:3291-300. 2005
    ..For the zinc-finger protein CTCF, a role in interpreting and propagating epigenetic states and in separating expression domains has been documented...
  27. ncbi The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso
    Pierre Antoine Defossez
    CNRS UMR5161, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
    J Biol Chem 280:43017-23. 2005
    CTC-binding factor (CTCF) is a DNA-binding protein of vertebrates that plays essential roles in regulating genome activity through its capacity to act as an enhancer blocker...
  28. ncbi Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism
    Verónica Torrano
    Grupo de Biología Molecular del Cáncer, Departamento de Biologia Molecular, Universidad de Cantabria, 39011 Santander, Spain
    J Cell Sci 119:1746-59. 2006
    Multiple functions have been reported for the transcription factor and candidate tumour suppressor, CTCF. Among others, they include regulation of cell growth, differentiation and apoptosis, enhancer-blocking activity and control of ..
  29. pmc CTCF binding at the H19 imprinting control region mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to Igf2
    Sreenivasulu Kurukuti
    Department of Development and Genetics, Uppsala University, Norbyvagen 18A, S 752 36 Uppsala, Sweden
    Proc Natl Acad Sci U S A 103:10684-9. 2006
    ..the H19 imprinting control region (ICR) directs the silencing of the maternally inherited Igf2 allele through a CTCF-dependent chromatin insulator...
  30. ncbi CTCF-dependent chromatin insulator is linked to epigenetic remodeling
    Ko Ishihara
    Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Mol Cell 23:733-42. 2006
    ..Here, we show that the SNF2-like chromodomain helicase protein CHD8 interacts with the insulator binding protein CTCF. Chromatin immunoprecipitation analysis revealed that CHD8 was present at known CTCF target sites, such as the ..
  31. pmc CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wide
    Igor Chernukhin
    Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Mol Cell Biol 27:1631-48. 2007
    b>CTCF is a transcription factor with highly versatile functions ranging from gene activation and repression to the regulation of insulator function and imprinting...
  32. ncbi Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch
    Mary E Donohoe
    Howard Hughes Medical Institute, USA
    Mol Cell 25:43-56. 2007
    ..Although no proven trans-acting factors have been identified, a likely candidate is Ctcf, a chromatin insulator with essential function in autosomal imprinting...
  33. pmc Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome
    Tae Hoon Kim
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA 92093 0653, USA
    Cell 128:1231-45. 2007
    ..In vertebrates, insulator's function requires association with the CCCTC-binding factor (CTCF), a protein that recognizes long and diverse nucleotide sequences...
  34. pmc Functional role of CCCTC binding factor (CTCF) in stress-induced apoptosis
    Tie Li
    Department of Medicine, HMC, David Geffen School of Medicine, University of California Los Angeles, Torrance, CA 90502, USA
    Exp Cell Res 313:3057-65. 2007
    b>CTCF, a nuclear transcriptional factor, is a multifunctional protein and involves regulation of growth factor- and cytokine-induced cell proliferation/differentiation...
  35. ncbi Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci
    Mario Renda
    Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy
    J Biol Chem 282:33336-45. 2007
    The DNA-binding protein CTCF (CCCTC binding factor) mediates enhancer blocking insulation at sites throughout the genome and plays an important role in regulating allele-specific expression at the Igf2/H19 locus and at other imprinted ..
  36. ncbi Functional role of CCCTC binding factor in insulin-stimulated cell proliferation
    J Gao
    Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, CA 90502, USA
    Cell Prolif 40:795-808. 2007
    CCCTC binding factor (CTCF) is a nuclear protein containing an 11-zinc-finger DNA-binding domain. CTCF plays important roles in the regulation of epigenetics and gene transcription...
  37. pmc Cohesins localize with CTCF at the KSHV latency control region and at cellular c-myc and H19/Igf2 insulators
    William Stedman
    Gene Regulation Program, The Wistar Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    EMBO J 27:654-66. 2008
    Cohesins, which mediate sister chromatin cohesion, and CTCF, which functions at chromatin boundaries, play key roles in the structural and functional organization of chromosomes...
  38. doi Cohesin mediates transcriptional insulation by CCCTC-binding factor
    Kerstin S Wendt
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Nature 451:796-801. 2008
    ..cohesin-binding sites in the human genome and show that most of these are associated with the CCCTC-binding factor (CTCF), a zinc-finger protein required for transcriptional insulation...
  39. doi Cohesins functionally associate with CTCF on mammalian chromosome arms
    Vania Parelho
    Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
    Cell 132:422-33. 2008
    ..occupy a subset of DNase I hypersensitive sites, many of which contain sequence motifs resembling the consensus for CTCF, a DNA-binding protein with enhancer blocking function and boundary-element activity...
  40. pmc The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions
    Parimal Majumder
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Exp Med 205:785-98. 2008
    Knockdown of the insulator factor CCCTC binding factor (CTCF), which binds XL9, an intergenic element located between HLA-DRB1 and HLA-DQA1, was found to diminish expression of these genes...
  41. pmc CTCF physically links cohesin to chromatin
    Eric D Rubio
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle WA 98195, USA
    Proc Natl Acad Sci U S A 105:8309-14. 2008
    ..proteomics approach, we show that the STAG1 (Scc3/SA1) subunit of cohesin interacts with the CCTC-binding factor CTCF bound to the c-myc insulator element...
  42. pmc The insulator binding protein CTCF positions 20 nucleosomes around its binding sites across the human genome
    Yutao Fu
    Bioinformatics Program, Boston University, Boston, Massachusetts, USA
    PLoS Genet 4:e1000138. 2008
    ..and we discovered an array of well-positioned nucleosomes flanking sites occupied by the insulator binding protein CTCF across the human genome. These nucleosomes are highly enriched for the histone variant H2A...
  43. pmc CTCF regulates allelic expression of Igf2 by orchestrating a promoter-polycomb repressive complex 2 intrachromosomal loop
    Tao Li
    Medical Service, VA Palo Alto Health Care System, Palo Alto, California 94304, USA
    Mol Cell Biol 28:6473-82. 2008
    b>CTCF is a zinc finger DNA-binding protein that regulates the epigenetic states of numerous target genes...
  44. pmc Global analysis of the insulator binding protein CTCF in chromatin barrier regions reveals demarcation of active and repressive domains
    Suresh Cuddapah
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genome Res 19:24-32. 2009
    ..They can be functionally classified as either enhancer blockers or domain barriers. CTCF (CCCTC-binding factor) is the only known major insulator-binding protein in the vertebrates and has been shown to ..
  45. pmc Cell cycle control of Kaposi's sarcoma-associated herpesvirus latency transcription by CTCF-cohesin interactions
    Hyojeung Kang
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Virol 83:6199-210. 2009
    ..studies have shown that this major latency control region is occupied by the cellular chromatin boundary factor CTCF and chromosome structural maintenance proteins SMC1, SMC3, and RAD21, which comprise the cohesin complex...
  46. pmc Epigenetic silencing of the p16(INK4a) tumor suppressor is associated with loss of CTCF binding and a chromatin boundary
    Michael Witcher
    Regulatory Biology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
    Mol Cell 34:271-84. 2009
    ..We show that the multifunctional protein CTCF associates in the vicinity of this boundary and absence of binding strongly coincides with p16 silencing in ..
  47. pmc Targeted deletion of multiple CTCF-binding elements in the human C-MYC gene reveals a requirement for CTCF in C-MYC expression
    Wendy M Gombert
    Black Lowe and Graham PLLC, Seattle, WA, USA
    PLoS ONE 4:e6109. 2009
    ..Previous studies examined the functional mechanism of the MYC insulator element MINE and its CTCF binding sites in the context of transgenes that were randomly inserted into the genome by transfection...
  48. pmc Epigenetic silencing in Friedreich ataxia is associated with depletion of CTCF (CCCTC-binding factor) and antisense transcription
    Irene De Biase
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America
    PLoS ONE 4:e7914. 2009
    ....
  49. pmc Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation
    Dawn Farrar
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom
    Mol Cell Biol 30:1199-216. 2010
    Poly(ADP-ribosyl)ation of the conserved multifunctional transcription factor CTCF was previously identified as important to maintain CTCF insulator and chromatin barrier functions...
  50. doi CTCF is a DNA methylation-sensitive positive regulator of the INK/ARF locus
    Carmen Rodriguez
    Institut de Recherche en Cancérologie de Montpellier INSERM Université de Montpellier I U896, CRLC Val d Aurelle Paul Lamarque, 34298 Montpellier Cedex 5, France
    Biochem Biophys Res Commun 392:129-34. 2010
    ..The resulting divergent promoter region is bound by the chromatin insulator protein CTCF in association with histone H3 tri-methylated on lysine 4, irrespective of transcription of ANRIL and ARF...
  51. doi Epigenetic regulation of the human p53 gene promoter by the CTCF transcription factor in transformed cell lines
    E Soto-Reyes
    Instituto de Fisiologia Celular, Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Mexico D F, Mexico
    Oncogene 29:2217-27. 2010
    ..We characterized a binding site for the CCCTC-binding factor (CTCF) in the human p53 gene promoter that contributes to its transcriptional expression, and has the ability to maintain ..
  52. pmc NF-kappaB subtypes regulate CCCTC binding factor affecting corneal epithelial cell fate
    Luo Lu
    Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Torrance, California 90502, USA
    J Biol Chem 285:9373-82. 2010
    CCCTC binding factor (CTCF) controls DNA imprinting, insulates important gene expression, and mediates growth factor- and stress-induced cell fate. However, regulatory mechanisms involved in intracellular CTCF activity are largely unknown...
  53. pmc Cell type specificity of chromatin organization mediated by CTCF and cohesin
    ChunHui Hou
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:3651-6. 2010
    b>CTCF sites are abundant in the genomes of diverse species but their function is enigmatic...
  54. pmc Gene-specific repression of the p53 target gene PUMA via intragenic CTCF-Cohesin binding
    Nathan P Gomes
    Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Genes Dev 24:1022-34. 2010
    ..Interestingly, the insulator protein CTCF (CCCTC-binding factor) and the Cohesin complex occupy these intragenic chromatin boundaries...
  55. pmc CCCTC-binding factor acts upstream of FOXA1 and demarcates the genomic response to estrogen
    Yu Zhang
    Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Peking University Health Science Center, Beijing 100191, China
    J Biol Chem 285:28604-13. 2010
    ..boundary elements surrounding the well defined ERalpha target TFF1 locus are occupied by the CCCTC-binding factor (CTCF)...
  56. pmc Distinct epigenetic domains separated by a CTCF bound insulator between the tandem genes, BLU and RASSF1A
    Jer Wei Chang
    Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    PLoS ONE 5:e12847. 2010
    ..3. We hypothesized that there may be a concordance on their gene expression and promoter methylation status. If not, then there may be an insulator located between RASSF1A and BLU genes that provides a barrier activity...
  57. doi CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancer
    Francesco Paolo Fiorentino
    Sbarro Health Research Organization, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    Mol Cancer Res 9:225-33. 2011
    ..fibroblasts the expression of Rb2/p130 gene is directly controlled by the chromatin insulator CCCTC-binding factor, CTCF, which by binding to the Rb2/p130 gene promoter induces, and/or maintains, a specific local chromatin organization ..
  58. pmc Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activity
    Tiaojiang Xiao
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell Biol 31:2174-83. 2011
    Recent studies have shown that the protein CTCF, which plays an important role in insulation and in large-scale organization of chromatin within the eukaryotic nucleus, depends for both activities on recruitment of the cohesin complex...
  59. pmc Interruption of intrachromosomal looping by CCCTC binding factor decoy proteins abrogates genomic imprinting of human insulin-like growth factor II
    He Zhang
    Department of Biochemistry and Molecular Biology, Ninth People s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
    J Cell Biol 193:475-87. 2011
    Monoallelic expression of IGF2 is regulated by CCCTC binding factor (CTCF) binding to the imprinting control region (ICR) on the maternal allele, with subsequent formation of an intrachromosomal loop to the promoter region...
  60. pmc Occupancy of chromatin organizers in the Epstein-Barr virus genome
    Meghan M Holdorf
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, CA 94158, USA
    Virology 415:1-5. 2011
    The human CCCTC-binding factor, CTCF, regulates transcription of the double-stranded DNA genomes of herpesviruses. The architectural complex cohesin and RNA Polymerase II also contribute to this organization...
  61. pmc Genome-wide studies of CCCTC-binding factor (CTCF) and cohesin provide insight into chromatin structure and regulation
    Bum Kyu Lee
    Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas, Austin, Texas 78712, USA
    J Biol Chem 287:30906-13. 2012
    ..CCCTC-binding factor (CTCF), a sequence-specific transcription factor, serves as a chromatin organizer in building this complex chromatin ..
  62. pmc CTCF/cohesin-mediated DNA looping is required for protocadherin α promoter choice
    Ya Guo
    Key Laboratory of Systems Biomedicine, Ministry of Education, Center for Comparative Biomedicine, Institute of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China
    Proc Natl Acad Sci U S A 109:21081-6. 2012
    ..In addition, we show that this DNA looping requires specific binding of the CTCF/cohesin complex to two symmetrically aligned binding sites in both the transcriptionally active promoters and in ..
  63. doi CTCF mediates the TERT enhancer-promoter interactions in lung cancer cells: identification of a novel enhancer region involved in the regulation of TERT gene
    Vegard Eldholm
    Department of Chemical and Biological Work Environment, National Institute of Occupational Health, Oslo, Norway
    Int J Cancer 134:2305-13. 2014
    ..TERT is embedded in a region of condensed chromatin and the chromatin remodeling protein CCCTC-binding factor (CTCF) has been implicated in the inhibition of TERT expression...
  64. pmc Cohesin and CTCF differentially affect chromatin architecture and gene expression in human cells
    Jessica Zuin
    Department of Cell Biology, Biophysical Genomics, Department of Cell Biology, Center for Biomics, Cancer Genomics Center, Erasmus Medical Center, 3015 GE, Rotterdam, The Netherlands
    Proc Natl Acad Sci U S A 111:996-1001. 2014
    ..The boundary sequences between domains are enriched for binding sites of CTCC-binding factor (CTCF) and the cohesin complex, implicating these two factors in the establishment or maintenance of topological domains...
  65. ncbi A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers
    G N Filippova
    Molecular Medicine Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Chromosomes Cancer 22:26-36. 1998
    ..We have cloned one such MYC regulatory factor, termed CTCF, which is a highly evolutionarily conserved-11-zinc finger transcriptional factor possessing multiple DNA sequence ..
  66. pmc The c-myc insulator element and matrix attachment regions define the c-myc chromosomal domain
    Wendy M Gombert
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98104, USA
    Mol Cell Biol 23:9338-48. 2003
    ..The enhancer-blocking activity is mediated by CTCF. Chromatin immunoprecipitation assays demonstrate that CTCF is constitutively bound at the insulator and at the ..
  67. pmc Epigenetic boundaries of tumour suppressor gene promoters: the CTCF connection and its role in carcinogenesis
    Felix Recillas-Targa
    Instituto de Fisiologia Celular, Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Mexico
    J Cell Mol Med 10:554-68. 2006
    ..We describe a previously unsuspected epigenetic factor and propose the incorporation of the 11-zinc finger CCCTC-binding factor, known as CTCF as a novel and multifunctional epigenetic regulator.
  68. ncbi Boundaries between chromosomal domains of X inactivation and escape bind CTCF and lack CpG methylation during early development
    Galina N Filippova
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Dev Cell 8:31-42. 2005
    ..We report that the 5' end of Jarid1c, a mouse escape gene adjacent to an inactivated gene, binds CTCF, displays high levels of histone H3 acetylation, and functions as a CTCF-dependent chromatin insulator...
  69. pmc Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq data
    Raja Jothi
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 36:5221-31. 2008
    ..by applying it on ChIP-Seq data for three widely studied and well-characterized human transcription factors: CTCF (CCCTC-binding factor), NRSF (neuron-restrictive silencer factor) and STAT1 (signal transducer and activator of ..
  70. ncbi Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development
    In Young Park
    Department of Biological Sciences, 373 1 Gusung Dong, Korea Advanced Institute of Science and Technology, Yusung Gu, Taejon 305 701, Republic of Korea
    J Cell Biochem 94:585-96. 2005
    ..The region containing two CCCTC binding factor (CTCF) binding sites in the 5'-ICR tended to be hypomethylated in the thymus of p53(-/-) mice, but not in the liver...
  71. ncbi Changes in the DNA methylation profile of the rat H19 gene upstream region during development and transgenic hepatocarcinogenesis and its role in the imprinted transcriptional regulation of the H19 gene
    Herbert Manoharan
    McArdle Laboratory for Cancer Research, Medical School, University of Wisconsin, Madison, Wisconsin 53706 1599, USA
    Mol Carcinog 41:1-16. 2004
    ..Similar to mouse and human, we showed that the zinc-finger CCTCC binding factor (CTCF) binds to the unmethylated CTCF binding site in the upstream region to influence monoallelic imprinted expression ..
  72. pmc CTCFL/BORIS is a methylation-independent DNA-binding protein that preferentially binds to the paternal H19 differentially methylated region
    Phuongmai Nguyen
    Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 68:5546-51. 2008
    The CTCF paralog BORIS (brother of the regulator of imprinted sites) is an insulator DNA-binding protein thought to play a role in chromatin organization and gene expression...
  73. ncbi Enhancer blocking by chicken beta-globin 5'-HS4: role of enhancer strength and insulator nucleosome depletion
    Hui Zhao
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:30573-80. 2006
    ..Nucleosome depletion at 5'-HS4 was dependent on interaction of the insulator protein CCCTC-binding factor (CTCF) and was required for enhancer blocking...
  74. pmc Dynamics of alpha-globin locus chromatin structure and gene expression during erythroid differentiation of human CD34(+) cells in culture
    Milind C Mahajan
    Department of Genetics, The Anlyan Center, Yale University School of Medicine, New Haven, CT 06510, USA
    Exp Hematol 37:1143-1156.e3. 2009
    ....
  75. pmc CTCF binding site classes exhibit distinct evolutionary, genomic, epigenomic and transcriptomic features
    Kobby Essien
    Penn Center for Bioinformatics, Department of Genetics, 415 Curie Boulevard, University of Pennsylvania, Philadelphia, PA 19104, USA
    Genome Biol 10:R131. 2009
    b>CTCF (CCCTC-binding factor) is an evolutionarily conserved zinc finger protein involved in diverse functions ranging from negative regulation of MYC, to chromatin insulation of the beta-globin gene cluster, to imprinting of the Igf2 locus...
  76. pmc Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene
    W W Quitschke
    Department of Psychiatry and Behavioral Science, State University of New York at Stony Brook, Stony Brook, NY 11794 8101, USA
    Nucleic Acids Res 28:3370-8. 2000
    High levels of transcription from the amyloid precursor protein promoter are dependent on the binding of CTCF to the APBbeta core recognition sequence located between positions -82 and -93 upstream from the transcriptional start site...
  77. pmc Systematic discovery of regulatory motifs in conserved regions of the human genome, including thousands of CTCF insulator sites
    Xiaohui Xie
    Broad Institute of MIT and Harvard, Massachusetts Institute of Technology and Harvard Medical School, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 104:7145-50. 2007
    ..We demonstrate by biochemical and computational methods that it defines a binding site for the CTCF protein, which is involved in insulator function to limit the spread of gene activation...
  78. ncbi CTCF-independent, but not CTCF-dependent, elements significantly contribute to TCR-alpha locus control region activity
    Janette Gomos-Klein
    Department of Biological Sciences, City University of New York, Hunter College, New York, NY 10021, USA
    J Immunol 179:1088-95. 2007
    ..Most vertebrate enhancer-blocking insulators are dependent on the CTCF transcription factor and its cognate DNA binding site...
  79. pmc MECP2 promoter methylation and X chromosome inactivation in autism
    Raman P Nagarajan
    Medical Microbiology and Immunology and Rowe Program in Human Genetics, University of California, Davis, CA 95616, USA
    Autism Res 1:169-78. 2008
    ..Chromatin immunoprecipitation analysis demonstrated that the CCTC-binding factor (CTCF) bound to this transition region in neuronal cells, consistent with a chromatin boundary at the methylation ..
  80. pmc Hierarchical hidden Markov model with application to joint analysis of ChIP-chip and ChIP-seq data
    Hyungwon Choi
    Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA
    Bioinformatics 25:1715-21. 2009
    ..Therefore, it is of interest to see whether the two data sources can be combined to enhance the detection of TFBS...
  81. pmc CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms
    E M Klenova
    Institute of Carcinogenesis, Russian Cancer Research Center, Moscow
    Mol Cell Biol 13:7612-24. 1993
    A novel sequence-specific DNA-binding protein, CTCF, which interacts with the chicken c-myc gene promoter, has been identified and partially characterized (V. V. Lobanenkov, R. H. Nicolas, V. V. Adler, H. Paterson, E. M. Klenova, A. V...
  82. ncbi Nucleosome positioning signals in the DNA sequence of the human and mouse H19 imprinting control regions
    C Davey
    Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King s Buildings, West Mains Road, Scotland EH9 3JR, Edinburgh, UK
    J Mol Biol 325:873-87. 2003
    ..to flank and encompass the short conserved sequences that constitute the binding sites for the zinc finger protein CTCF, an essential mediator of insulator activity...
  83. pmc Modular insulators: genome wide search for composite CTCF/thyroid hormone receptor binding sites
    Oliver Weth
    Institute for Genetics, Justus Liebig University Giessen, Giessen, Germany
    PLoS ONE 5:e10119. 2010
    The conserved 11 zinc-finger protein CTCF is involved in several transcriptional mechanisms, including insulation and enhancer blocking...
  84. pmc The pluripotency factor Oct4 interacts with Ctcf and also controls X-chromosome pairing and counting
    Mary E Donohoe
    Howard Hughes Medical Institute, USA
    Nature 460:128-32. 2009
    ..Xite, two regulatory noncoding RNA genes of the X-inactivation centre, and also complexes with XCI trans-factors, Ctcf and Yy1 (ref. 17), through protein-protein interactions...
  85. doi Analysis of the IGF2/H19 imprinting control region uncovers new genetic defects, including mutations of OCT-binding sequences, in patients with 11p15 fetal growth disorders
    Julie Demars
    Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, Melbourne, 3004 VIC, Australia
    Hum Mol Genet 19:803-14. 2010
    ..5. This methylation-sensitive chromatin insulator works by binding the zinc-finger protein CTCF in a parent-specific manner...
  86. pmc Disruption of genomic neighbourhood at the imprinted IGF2-H19 locus in Beckwith-Wiedemann syndrome and Silver-Russell syndrome
    Raffaella Nativio
    Department of Oncology, Cancer Research UK Cambridge Research Institute, University of Cambridge, LiKaShing Centre, Cambridge, UK
    Hum Mol Genet 20:1363-74. 2011
    ..of the ICR controls the reciprocal imprinting of IGF2 and H19 by preventing the binding of the insulator protein, CTCF. We here show that local changes in histone modifications and CTCF--cohesin binding at the ICR in BWS and SRS ..
  87. ncbi Regulatory factor interactions and somatic silencing of the germ cell-specific ALF gene
    Minjung Kim
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas 75080, USA
    J Biol Chem 281:34288-98. 2006
    ..Two additional complexes (C1 and C3) are due to the multivalent zinc finger protein CTCF, a factor that plays a role in gene silencing and chromatin insulation...
  88. pmc Chromatin profiling of Epstein-Barr virus latency control region
    Latasha Day
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    J Virol 81:6389-401. 2007
    ..binding patterns of EBNA1 with those of the origin recognition complex protein ORC2, the chromatin boundary factor CTCF, the linker histone H1, and several histone modifications...
  89. ncbi Duplication of the paternal IGF2 allele in trisomy 11 and elevated expression levels of IGF2 mRNA in congenital mesoblastic nephroma of the cellular or mixed type
    Naoki Watanabe
    Department of Cancer Diagnosis, Research Institute for Clinical Oncology, Saitama Cancer Center, Ina, Saitama, and Department of Pediatrics, Juntendo University, Nerima Hospital, Tokyo, Japan
    Genes Chromosomes Cancer 46:929-35. 2007
    ..with disomy 11 showed one paternal and one maternal allele of IGF2, analyzing the methylation status of the sixth CTCF site of the H19-differentially methylated region...
  90. pmc Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosis
    Jun ichiro Komura
    Department of Cell Biology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980 8575, Japan
    Proc Natl Acad Sci U S A 104:15741-6. 2007
    ..Recently, it has been reported that CTCF, a major protein in vertebrate insulator elements, remains associated with mitotic chromatin...
  91. doi Evolutionary rate heterogeneity of Alu repeats upstream of the APOA5 gene: do they regulate APOA5 expression?
    Edward A Ruiz-Narváez
    Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    J Hum Genet 53:247-53. 2008
    ..Also, we scanned the intergenic region for the presence of binding sites of the insulator protein CTCF. Seven out of the nine found CTCF binding sites were located in the first half of the intergenic region...
  92. pmc Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved
    Jenny M Rhodes
    Department of Pathology, Dunedin School of Medicine, The University of Otago, Dunedin, New Zealand
    Dev Biol 344:637-49. 2010
    ..Roles for cohesin in regulating gene expression, sometimes in combination with CCCTC-binding factor (CTCF), have emerged...
  93. pmc A DNA insulator prevents repression of a targeted X-linked transgene but not its random or imprinted X inactivation
    Dominic Ciavatta
    Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 103:9958-63. 2006
    ..Thus, the chicken hypersensitive site 4 DNA insulator is sufficient to protect an X-linked gene from repression during development but not from X inactivation...
  94. ncbi IGF2/H19 hypomethylation in Silver-Russell syndrome and isolated hemihypoplasia
    Michael Zeschnigk
    Institut fur Humangenetik, Universitatsklinikum Essen, Essen, Germany
    Eur J Hum Genet 16:328-34. 2008
    ..found to be hypomethylated, as determined by Southern blot analysis of an HpaII restriction site close to the third CTCF-binding site (CTS3) within ICR1...
  95. doi Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia
    C J Marques
    Department of Genetics, Faculty of Medicine, Porto 4200 319, Portugal
    Mol Hum Reprod 14:67-74. 2008
    ..54% (1.2-8.3%) and complete unmethylation in 2.95% (0-5.9%) of H19 clones. Similarly, for the CTCF-binding site 6, hypomethylation occurred in 4.8% (1.2-8.9%) and complete unmethylation in 3.7% (0-6...
  96. pmc Epigenetic regulation of latent HSV-1 gene expression
    David C Bloom
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610 0266, USA
    Biochim Biophys Acta 1799:246-56. 2010
    ..Binding of CTCF, a protein that binds to all known vertebrate insulators, to sites within the HSV-1 genome likely prevents ..
  97. doi Epigenetic control of expression of the human L- and M- pigment genes
    S S Deeb
    Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA, USA
    Ophthalmic Physiol Opt 30:446-53. 2010
    ..This DHS region contains a highly conserved motif that binds CCCTC-binding factor (CTCF), referred to as a 'chromatin insulator or boundary element', that has been shown to regulate gene expression at ..
  98. doi Epigenetic regulation of latent Epstein-Barr virus promoters
    Maria Takacs
    Division of Virology, National Center for Epidemiology, H 1097 Budapest, Gyali ut 2 6, Hungary
    Biochim Biophys Acta 1799:228-35. 2010
    ..We speculate that binding of the chromatin insulator protein CTCF to 3 distinct sites (within, close to and far from the matrix attachment region) may contribute to the three-..
  99. ncbi Loss of imprinting and cancer
    P Jelinic
    Division of Experimental Pathology, University Institute of Pathology, Lausanne, Switzerland
    J Pathol 211:261-8. 2007
    ..Although the major reading mechanisms of imprinted status are known, chromatin boundary formation by CTCF and untranslated RNAs, the molecules elaborating the initial ICR methylation, are just being uncovered...
  100. pmc The CTCF insulator protein is posttranslationally modified by SUMO
    Melissa J MacPherson
    Department of Molecular Genetics, 1 King s College Circle, University of Toronto, Toronto, ON, Canada
    Mol Cell Biol 29:714-25. 2009
    The CTCF protein is a highly conserved zinc finger protein that is implicated in many aspects of gene regulation and nuclear organization. Its functions include the ability to act as a repressor of genes, including the c-myc oncogene...
  101. pmc Negative protein 1, which is required for function of the chicken lysozyme gene silencer in conjunction with hormone receptors, is identical to the multivalent zinc finger repressor CTCF
    M Burcin
    , Giessen, Germany
    Mol Cell Biol 17:1281-8. 1997
    ..Another protein, CCCTC-binding factor (CTCF), specifically binds to 50-bp-long sequences that contain repetitive CCCTC elements in the vicinity of vertebrate c-..

Research Grants62

  1. Jean Pierre J Issa; Fiscal Year: 2016
    ..to DNA methylation (the transcription factor Sp1, LINE/SINE retrotransposons and the insulator proteins CTCF, USF1/2 and VEZF1) will be tested in specific aim 2...
  2. SHAOCHUNG VICTOR HSIA; Fiscal Year: 2016
    ..genes by recruiting multifunctional transcription factor Early Growth Response gene (Egr-1) and chromatin insulator CTCF to the key regulatory regions of HSV-1...
  3. Ann Harris; Fiscal Year: 2015
    ..that likely establish the looped conformation of the active locus and determine how trans-acting factors such as CTCF and cohesin influence this spatial organization...
  4. Epigenetic Regulation of Serotonin:Relevance to HIV and Methamphetamine Abuse
    GREGORY MICHAEL MILLER; Fiscal Year: 2012
    ..We will also assess the involvement of DNA methylation and CCCTC-binding factor (CTCF) in the regulation of TPH2 gene expression...
  5. Epigenetic Regulation of Imprinting in Mouse Embryo
    Marisa S Bartolomei; Fiscal Year: 2012
    ..Specific Aim 1 will examine the general role of the multi-functional protein CTCF, which provides enhancer blocking function and a DNA methylation-free region on the imprinting control region (ICR) ..
  6. Paul M Lieberman; Fiscal Year: 2016
    ..protein Rb (aim1), the viral- encoded origin binding protein EBNA1 (aim 2), and the chromatin boundary factor CTCF (aim 3)...
  7. Marisa S Bartolomei; Fiscal Year: 2016
    ..The ICR, which is also designated the differentially methylated domain (DMD), acts as a methylation-senstitive, CTCF-dependent insulator...
  8. Tae Hoon Kim; Fiscal Year: 2014
    ..We have found that the insulator protein CTCF binding sites define precise molecular boundaries at a number of large heterochromatin domains found in the human ..
  9. EPIGENETIC REGULATION IN A CANCER ASSOCIATED REGION
    Michael J Higgins; Fiscal Year: 2012
    ..Moreover, we have shown that the epigenetic regulatory protein CTCF binds to the minimal repressive sequence in an allele-specific manner...
  10. Jeremy M Boss; Fiscal Year: 2015
    ..MHC-II gene transcription and these interactions are dependent on the insulator-binding factor CTCF. Our observations establish a new paradigm for how these genes are regulated...
  11. Role of CTCF in V(D)J Rearrangement
    STEPHANIE CHRISTINE DEGNER; Fiscal Year: 2010
    ..The nuclear factors controlling Ig loci contraction are not known. CTCF (CCCTC-binding factor) is a nuclear protein that participates in long-range chromosomal interactions...
  12. Library Screening for Novel Enhancer Blockers Derived from Human T Lymphocytes
    Brian P Sorrentino; Fiscal Year: 2013
    ..Jude for homologous gene targeting in Jurkat T cells to screen a library of CTCF binding sequences derived from T cells for enhancer blocking activity...
  13. Paul M Lieberman; Fiscal Year: 2015
    ..that this region of the viral chromosome is protected from epigenetic silencing by the chromatin insulator protein CTCF and colocalization with cohesin subunits (e.g. SMC1, SMC3, Rad21)...
  14. BEVERLY MARIE EMERSON; Fiscal Year: 2015
    ..We find that loss of CTCF-dependent chromatin boundaries, which protects genes from adjacent heterochromatin domains, results in ..
  15. FUNCTION OF THE SUBUNITS OF EUKARYOTIC CHROMOSOMES
    WILLIAM GARRARD; Fiscal Year: 2013
    ..4. To determine the impact of separately mutating Ikaros and CTCF binding sites within Sis on Ig loci nuclear organization, distal-Sis-interacting sequences, and repertoire.
  16. Christine M Disteche; Fiscal Year: 2016
    ..We discovered two regions that bind CTCF only on the inactive X and produce non-coding RNAs that escape X inactivation...
  17. Regulation of HSV-1 gene expression and reactivation by insulator protein CTCF
    Donna M Neumann; Fiscal Year: 2013
    ..We have also identified clusters of DNA binding motifs for the cellular insulating protein, CTCF around genomic regions of HSV-1 that are critical for both the establishment of latency as well as in reactivation...
  18. Heterochromatin on the human inactive X chromosome
    Brian P Chadwick; Fiscal Year: 2012
    ..Intriguingly, the chromatin insulator and epigenetic organizer protein CTCF binds specifically to the Xi array, immediately adjacent to the DXZ4 bi-directional promoter...
  19. Duplin: A Novel A-Kinase Anchoring Protein
    MAUREEN E O apos DONNELL; Fiscal Year: 2010
    ..in the embryonic mouse, and is required for chromatin insulation in conjunction with the transcription represser CTCF. Duplin/Chd8 has been characterized as a developmental protein, but is also expressed adult tissue...
  20. Elliott D Kieff; Fiscal Year: 2016
    ..also co-activated genome wide by EBNALP, which binds mostly to promoters, which are marked by the presence of YY1, CTCF, and ZNF143, as well as Histone H2-Az...
  21. Mary E Donohoe; Fiscal Year: 2014
    ..The role of the chromatin insulator Ctcf and its protein partners will be investigated in the transcriptional regulation of MeCP2...
  22. Nuclear organization in stem and differentiated cells
    Victor G Corces; Fiscal Year: 2010
    ..Preliminary results in our lab suggest that the organization of CTCF insulators in mouse stem cells changes as these cells differentiate...
  23. Joaquin M Espinosa; Fiscal Year: 2016
    ..Each of these mechanisms will be further investigated by developing the following Specific Aims: 1. To define how CTCF, Polycomb complexes and lncRNAs regulate PUMA...
  24. Regulation of EBV Latency by Chromosome Conformation
    Italo Tempera; Fiscal Year: 2012
    ..Moreover, we found that the cellular factor CTCF binds at several key regions of the EBV genome and regulates promoter selection and viral gene expression...
  25. CCCTC-binding factor (CTCF) in trinucleotide repeat instability and disease
    Albert R La Spada; Fiscal Year: 2010
    ..Within this 3'region is a binding site for the "CCCTC-binding factor" (CTCF), a protein with a variety of functions that stem from its ability to modulate DNA structure...
  26. Aaron F Straight; Fiscal Year: 2016
    ..new methods to measure the formation of looped domains dependent upon the activity of the chromatin protein CTCF. CTCF is known to be required for the stabilization of looped regions in the genome but how it generates or ..
  27. MARTIN JOHN WALSH; Fiscal Year: 2014
    ..First, we will investigate the role of CHD6 to cooperate with additional transcriptional proteins such as CTCF to regulate CFTR...
  28. Next generation sequence analysis of the IgH repertoire
    Ann J Feeney; Fiscal Year: 2012
    ..Therefore, we will compare rearrangement frequencies to the locations of CTCF/cohesin sites...
  29. MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATE
    David F Jarrard; Fiscal Year: 2013
    ..of IGF2 LOI on prostate carcinogenesis in a susceptible mouse, ii) determining whether interventions that induce CTCF maintain IGF2 imprinting, and iii) define and validate IGF2 LOI in the `field defect'associated with human ..
  30. Italo Tempera; Fiscal Year: 2014
    ..Moreover, we found that the cellular factor CTCF binds at several key regions of the EBV genome and regulates promoter selection and viral gene expression...
  31. Michael S Krangel; Fiscal Year: 2015
    ..manipulated strains of mice will be used to test the roles of TCR locus enhancers and the roles of proteins CTCF and cohesin in defining locus conformational states...
  32. Ann J Feeney; Fiscal Year: 2014
    ..This is accomplished by contraction of the Vh locus via multiple chromosomal looping events. CTCF is a zinc finger protein that is associated with insulators and boundary elements at several well-studied ..
  33. John A Stamatoyannopoulos; Fiscal Year: 2016
    ..therein, and to continue to provide relevant synergistic annotations including RNA-seq, histone modifications, and CTCF, as well as DNA methylation. The overriding focus of our production effort has been on data quality...
  34. Stephen J Tapscott; Fiscal Year: 2016
    ..The aims of Project 3 (Dr. Filippova) will be to determine whether CTCF binding on the disease associated allele regulates regional chromatin structure, RNA transcription, or nuclear ..
  35. CTCF peptides - a new therapeutic alternative to target HIV in the brain.
    Malgorzata Simm; Fiscal Year: 2012
    ..Recently we identified the HRF as a truncated form of a potent transcription suppressor - CTCF (tCTCF)...
  36. Frederick W Alt; Fiscal Year: 2015
    ..We have found that the 5'D4KBS functionally employs a CTCF looping/insulator factor binding site, at least for its lineage-specific function...
  37. Carl L Schildkraut; Fiscal Year: 2016
    ..We will also determine the contribution of CTCF binding to FMR1 replication programs...
  38. VISUAL NEURONS--DEVELOPMENT AND REGENERATION IN VITRO
    MARIA VALERIA CANTO SOLER; Fiscal Year: 2010
    ..Functional analysis will focus on CTCF, a multifunctional member of the Zn-finger family of transcriptional regulators, which is hypothesized to act as a ..
  39. MARIA VALERIA CANTO-SOLER; Fiscal Year: 2016
    ..Within this framework, we have identified CTCF, a multifunctional transcriptional regulator previously unknown to participate in these phenomena, as a leading ..
  40. Role of regulatory sequence at the H19 imprinting control region
    Folami Ideraabdullah; Fiscal Year: 2009
    ..Previous studies have shown that binding sites for the insulator protein CTCF present at the ICR are necessary for imprinting control...
  41. The Role of CTG Repeats in DM1 Locus Regulation
    SARA GEORGES; Fiscal Year: 2006
    ..My central hypothesis is that the precise establishment of chromatin structure by wild type CTG repeats and CTCF binding sites at the DM1 insulator is critical for the maintenance of transcriptional regulation at the DM1 locus, ..
  42. STUDY ON A NOVEL ALZHEIMER APP REGULATORY FACTOR
    Wolfgang Quitschke; Fiscal Year: 2006
    ..This element binds the multivalent and multifunctional transcription factor CTCF, which acts as a transcriptional activator...
  43. Analysis of transcription in lens using tiled microarrays (ChIP on chip)
    Ales Cvekl; Fiscal Year: 2007
    ..focusing on histone H3 K9 acetylation, H3 K3/27 methylation, methylation of DMA, binding of insulator protein CTCF and chromatin activator HMGN3, all involved in epigenetic regulation...
  44. Evolutionary analysis of CTCF and potential links to breast cancer phenotypes
    Christopher Carlson; Fiscal Year: 2007
    ..One region with frequent loss of heterozygosity in breast cancer is chromosome 16q22 [6], within which the CTCF multifunctional transcription factor lies, and CTCF expression in breast cancer cells has been associated with ..
  45. Genetic and Epigenetic Markers in Ovarian Cancer
    Elizabeth Swisher; Fiscal Year: 2006
    ..In ovarian cancers, evaluate expression of BRCA1 and other proteins that may interact with BRCA1 including Id4, CTCF, and ATM, correlating protein expression with clinical outcomes in both hereditary and sporadic ovarian cancer...
  46. Epigenetic effects if endocrine disruptors in fetal germ cells
    PIROSKA E SZABO; Fiscal Year: 2010
    ....
  47. 2006 Mammalian Gametogenesis/Embryogenesis Gordon
    Marisa Bartolomei; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  48. Genetically Modified Rhesus Monkeys
    Shoukhrat Mitalipov; Fiscal Year: 2006
    ..Such animals should provide a resource for the study of human neurogenetic diseases and serve as pre-clinical models for new experimental treatments including gene and stem cell based therapies. ..
  49. Quantitating chromatin dynamics during erythropoiesis
    M Bender; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  50. Mouse Models of Tumor Progression and Therapy Response
    CHRISTOPHER KEMP; Fiscal Year: 2007
    ..These experiments will reveal the molecular and cellular basis for the poor clinical outcome associated with p27 deficient tumors. This knowledge can then be applied to improve treatment design and regimen in the clinical setting. ..
  51. Chromatin Insulator Function at the C-myc Gene
    Anton Krumm; Fiscal Year: 2007
    ..The results will broaden our understanding of the role of chromatin elements in the specific regulation and nuclear organization of mammalian oncogenes in normal and malignant cells. ..
  52. MOLECULAR GENETICS OF COLOR VISION
    Samir Deeb; Fiscal Year: 2008
    ..This will also help in developing cell-specific gene therapy strategies. ..
  53. Maternal-Fetal Conflict: The Effect of Imprinted Genes on Fetal Growth
    Ronald M Adkins; Fiscal Year: 2010
    ..Dietary survey instruments during pregnancy and just afterwards will be used to determine the maternal intake of nutrients vital to DNA methylation. Statistical analyses will be performed to test the three hypotheses. ..
  54. The Role of Double Strand Breaks in Carcinogenesis
    CHRISTOPHER KEMP; Fiscal Year: 2006
    ..Understanding the functional interaction of the P13 Ks, in regulating p53, apoptosis, development and carcinogenesis at the level of the whole animal is a necessary link to apply knowledge gained from cell culture models to the clinic. ..
  55. FSHD: Chromatin Structure, Looping, & Expression
    Melanie Ehrlich; Fiscal Year: 2009
    ..The proposed research should elucidate new aspects of long-distance control of gene expression as well as lending clinically useful insights into this currently intractable disease. ..
  56. Genomics and Epigenomics of Fetal Growth Regulation
    Ronald M Adkins; Fiscal Year: 2010
    ..Statistical analyses will be performed to test the three hypotheses. Note: The only change from the original abstract is a change from 1,000 to 800 mothernewborn pairs. ..
  57. Gene Activation by Remote Transcriptional Enhancers
    Dale Dorsett; Fiscal Year: 2004
    ..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
  58. EARLY RESPONSE OF CORNEAL EPITHELIUM TO UV-INDUCED DEATH
    Luo Lu; Fiscal Year: 2004
    ..Furthermore therapeutic measures may be identified which could reduce the incidence of UV-induced apoptotic corneal epithelial damage and increases in corneal epithelial susceptibility to infection and diseases. ..
  59. Structure and Function of CTCF: Mouse Model Studies
    Galina Filippova; Fiscal Year: 2007
    DESCRIPTION (provided by applicant): CTCF is a highly conserved 11 Zn finger (ZF) transcription factor and insulator protein...
  60. MOLECULAR GENETICS OF COLOR VISION
    Samir S Deeb; Fiscal Year: 2010
    ..Selective Tr2 ligands could potentially be designed to correct abnormal phenotypes during retinal development. ..
  61. SEQUENCES CONTROLLING H19 GENE IMPRINTING
    Marisa S Bartolomei; Fiscal Year: 2010
    ..DMD), is hypermethylated on the repressed paternal allele and acts as a methylation-sensitive insulator through CTCF-binding on the maternal allele...

Patents1

  1. T cell activating gene
    Patent Number: WO2004058805-A2; Date:2004-07-15