CrkL

Summary

Gene Symbol: CrkL
Description: CRK like proto-oncogene, adaptor protein
Alias: crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
Species: human

Top Publications

  1. ncbi Sos, Vav, and C3G participate in B cell receptor-induced signaling pathways and differentially associate with Shc-Grb2, Crk, and Crk-L adaptors
    L Smit
    Division of Cellular Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 271:8564-9. 1996
  2. doi MUC1 initiates Src-CrkL-Rac1/Cdc42-mediated actin cytoskeletal protrusive motility after ligating intercellular adhesion molecule-1
    Qiang Shen
    Department of Laboratory Medicine and Pathology, 5B4 21 Mackenzie Health Sciences Centre, University of Alberta Hospital, Edmonton, AB, Canada T6G 2R7
    Mol Cancer Res 6:555-67. 2008
  3. ncbi Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells
    T B van Dijk
    Institute of Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands
    Blood 96:3406-13. 2000
  4. ncbi Involvement of hematopoietic progenitor kinase 1 in T cell receptor signaling
    P Ling
    Department of Immunology and the Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 276:18908-14. 2001
  5. ncbi Stimulation through the T cell receptor induces Cbl association with Crk proteins and the guanine nucleotide exchange protein C3G
    K A Reedquist
    Lymphocyte Biology Section, Division of Rheumatology and Immunology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 271:8435-42. 1996
  6. ncbi The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene
    K Senechal
    Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 271:23255-61. 1996
  7. ncbi Tyrosine 207 in CRKL is the BCR/ABL phosphorylation site
    R De Jong
    Department of Pathology, Childrens Hospital of Los Angeles, CA 90027, USA
    Oncogene 14:507-13. 1997
  8. ncbi Maintenance of human T cell anergy: blocking of IL-2 gene transcription by activated Rap1
    V A Boussiotis
    Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 278:124-8. 1997
  9. ncbi Association of the Cas-like molecule HEF1 with CrkL following integrin and antigen receptor signaling in human B-cells: potential relevance to neoplastic lymphohematopoietic cells
    A Astier
    Division of Hematologic Malignancies, Dana Farber Cancer Institute, Philadelphia, PA, USA
    Leuk Lymphoma 28:65-72. 1997
  10. ncbi CBL-GRB2 interaction in myeloid immunoreceptor tyrosine activation motif signaling
    R K Park
    Division of Hematology Oncology, Children s Hospital Los Angeles Research Institute and University of Southern California School of Medicine, Norris Cancer Center, 90027, USA
    J Immunol 160:5018-27. 1998

Research Grants

  1. CLONING OF THE Hol MUTATION
    Licia Selleri; Fiscal Year: 2009
  2. Developmental Genetics of the Pharyngeal Apparatus
    Bernice E Morrow; Fiscal Year: 2010
  3. Janet C King; Fiscal Year: 2014
  4. KERIANN MARIE BACKUS; Fiscal Year: 2014
  5. Genetic Basis of Birth Defects in 22q11 Deletion Syndrome
    Bernice E Morrow; Fiscal Year: 2010
  6. Jak2 involvement in Bcr-Abl oncogenic transformation
    Ralph Arlinghaus; Fiscal Year: 2005
  7. Stat5 dephosphorylation by Shp-2
    Demin Wang; Fiscal Year: 2007
  8. Mechanism of RIN1 Signaling in Neuronal Plasticity
    John Colicelli; Fiscal Year: 2007
  9. BCR AND ABL RELATED GENES
    John Groffen; Fiscal Year: 1999
  10. The Role of CbL in AbL Signaling and Leukemia
    Hua Gu; Fiscal Year: 2006

Scientific Experts

Detail Information

Publications206 found, 100 shown here

  1. ncbi Sos, Vav, and C3G participate in B cell receptor-induced signaling pathways and differentially associate with Shc-Grb2, Crk, and Crk-L adaptors
    L Smit
    Division of Cellular Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 271:8564-9. 1996
    ..Since Cbl binds to Grb2, Crk, as well as Crk-L, we hypothesize that Cbl may affect the function of all three exchangers...
  2. doi MUC1 initiates Src-CrkL-Rac1/Cdc42-mediated actin cytoskeletal protrusive motility after ligating intercellular adhesion molecule-1
    Qiang Shen
    Department of Laboratory Medicine and Pathology, 5B4 21 Mackenzie Health Sciences Centre, University of Alberta Hospital, Edmonton, AB, Canada T6G 2R7
    Mol Cancer Res 6:555-67. 2008
    ..lamellipodial and filopodial protrusions require Src family kinase and CT10 regulator of kinase like (CrkL) accompanied by the rapid formation of a Src-CrkL signaling complex at the MUC1 cytoplasmic domain...
  3. ncbi Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells
    T B van Dijk
    Institute of Hematology, Erasmus University Rotterdam, Rotterdam, The Netherlands
    Blood 96:3406-13. 2000
    ..Dok-1 was demonstrated to bind to the SH2 domains of several signaling molecules activated by SCF, including Abl, CrkL, SHIP, and PLCgamma-1, but not those of Vav and Shc...
  4. ncbi Involvement of hematopoietic progenitor kinase 1 in T cell receptor signaling
    P Ling
    Department of Immunology and the Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 276:18908-14. 2001
    ..the adapters Nck and Crk with different kinetics, whereas it constitutively interacted with the adapters Grb2 and CrkL in Jurkat T cells...
  5. ncbi Stimulation through the T cell receptor induces Cbl association with Crk proteins and the guanine nucleotide exchange protein C3G
    K A Reedquist
    Lymphocyte Biology Section, Division of Rheumatology and Immunology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 271:8435-42. 1996
    ..Here we show that Cbl associates with all three forms of the human Crk protein, predominantly CrkL, following T cell receptor activation of Jurkat T cells...
  6. ncbi The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene
    K Senechal
    Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 271:23255-61. 1996
    The CRKL adaptor protein was recently identified as a substrate for the BCR-ABL tyrosine kinase in patients with chronic myelogenous leukemia, but its function is unknown...
  7. ncbi Tyrosine 207 in CRKL is the BCR/ABL phosphorylation site
    R De Jong
    Department of Pathology, Childrens Hospital of Los Angeles, CA 90027, USA
    Oncogene 14:507-13. 1997
    BCR/ABL has a causal role in Philadelphia (Ph)-chromosome positive leukemia. The SH2/SH3 adapter protein CRKL is a major substrate of the deregulated BCR/ABL tyrosine kinase and is aberrantly tyrosine-phosphorylated in Ph-positive ..
  8. ncbi Maintenance of human T cell anergy: blocking of IL-2 gene transcription by activated Rap1
    V A Boussiotis
    Department of Adult Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 278:124-8. 1997
    ..The adapter protein CrkL was associated with both phosphorylated Cbl and the guanidine nucleotide-releasing factor C3G, which catalyzes ..
  9. ncbi Association of the Cas-like molecule HEF1 with CrkL following integrin and antigen receptor signaling in human B-cells: potential relevance to neoplastic lymphohematopoietic cells
    A Astier
    Division of Hematologic Malignancies, Dana Farber Cancer Institute, Philadelphia, PA, USA
    Leuk Lymphoma 28:65-72. 1997
    b>CrkL, a cellular homologue of the v-crk oncogene, belongs to the family of adaptor proteins, containing SH2 and SH3 domains, but no catalytic domain...
  10. ncbi CBL-GRB2 interaction in myeloid immunoreceptor tyrosine activation motif signaling
    R K Park
    Division of Hematology Oncology, Children s Hospital Los Angeles Research Institute and University of Southern California School of Medicine, Norris Cancer Center, 90027, USA
    J Immunol 160:5018-27. 1998
    ..Glutathione-S-transferase fusion protein data demonstrate that the constitutive interaction of CBL with GRB2 and CRKL is mediated via two discrete regions of the CBL C terminus...
  11. ncbi Interplay of the proto-oncogene proteins CrkL and CrkII in insulin-like growth factor-I receptor-mediated signal transduction
    A P Koval
    Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 273:14780-7. 1998
    The closely related proto-oncogene proteins CrkII and CrkL consist of one SH2 and two SH3 domains and share 60% overall homology with the highest identity within their functional domains...
  12. pmc Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells
    K Senechal
    Department of Medicine, University of California Los Angeles, Los Angeles, California, USA
    Mol Cell Biol 18:5082-90. 1998
    b>Crkl is an adapter protein and phosphotyrosine-containing substrate implicated in transformation by the bcr-abl oncogene and in signaling by cytokines...
  13. ncbi Interactions of p62(dok) with p210(bcr-abl) and Bcr-Abl-associated proteins
    A Bhat
    Division of Hematology and Medical Oncology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Biol Chem 273:32360-8. 1998
    ..by p210(bcr-abl), p62(dok) is tyrosine-phosphorylated and co-immunoprecipitates with Bcr-Abl, RasGAP, and CrkL, a Src homology 2 (SH2) and SH3 domain-containing adaptor protein...
  14. ncbi Physiological signals and oncogenesis mediated through Crk family adapter proteins
    S M Feller
    Laboratory of Molecular Oncology, MSZ Institute for Medical Radiation and Cell Research, University Würzberg, Germany
    J Cell Physiol 177:535-52. 1998
    ..oncogene (v-Crk) is known to induce sarcomas in chicken and its cellular homologs c-Crk I, c-Crk II, and Crk-like (CRKL) have been implicated in many signal transduction events...
  15. ncbi Cbl functions downstream of Src kinases in Fc gamma RI signaling in primary human macrophages
    A Erdreich-Epstein
    Neil Bogart Memorial Laboratories, Department of Pediatrics, Childrens Hospital Los Angeles Research Institute, University of Southern California School of Medicine, USA
    J Leukoc Biol 65:523-34. 1999
    ..is rapidly tyrosine phosphorylated in response to Fc receptor activation and binds to the adaptor proteins Grb2, CrkL, and Nck. A few reports describe Cbl interactions in primary human hematopoietic cells...
  16. ncbi Role of Src in the modulation of multiple adaptor proteins in FcalphaRI oxidant signaling
    R K Park
    Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA
    Blood 94:2112-20. 1999
    ..that FcalphaR-mediated signals modulate a multimolecular adaptor protein complex containing Grb2, Shc, SHIP, CrkL, Cbl, and SLP-76...
  17. ncbi The adapter protein Crkl links Cbl to C3G after integrin ligation and enhances cell migration
    N Uemura
    Department of Adult Oncology, Dana Farber Cancer Institute, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 274:37525-32. 1999
    b>Crkl, an SH2-SH3-SH3 adapter protein, is one of the major tyrosine phosphoproteins detected in cells from patients with chronic myelogenous leukemia...
  18. ncbi Signaling of hepatocyte growth factor/scatter factor (HGF) to the small GTPase Rap1 via the large docking protein Gab1 and the adapter protein CRKL
    D Sakkab
    Laboratory of Molecular Oncology, MSZ Institut für Medizinische Strahlenkunde und Zellforschung, Universitat Wurzburg, D 97078 Wurzburg, Germany
    J Biol Chem 275:10772-8. 2000
    ..YXXP) motifs which, when phosphorylated, are potential binding sites for the adapter proteins c-Crk and Crk-like (CRKL). Stimulation of human embryonic kidney cells (HEK293) with HGF leads to Gab1 association with CRKL...
  19. pmc Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules
    Takeshi Nakamura
    Department of Molecular Genetics, National Institute for Longevity Sciences, Program of Protecting the Brain, CREST, JST, Oobu, Aichi 474 8522, Japan
    Mol Cell Biol 22:8721-34. 2002
    ..Another pool of Grit was recruited to the activated receptor tyrosine kinase through its binding to N-Shc and CrkL/Crk, adapter molecules downstream of activated receptor tyrosine kinases...
  20. pmc Translocation of CrkL to focal adhesions mediates integrin-induced migration downstream of Src family kinases
    Leiming Li
    Committee on Cell Physiology and The Ben May Institute for Cancer Research and Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
    Mol Cell Biol 23:2883-92. 2003
    The adapter protein Crk-Like (CrkL) can associate with the Src substrate p130(Cas) (Cas). The biological role of CrkL downstream of Cas, however, has been largely obscure...
  21. pmc Negative regulation of Rap1 activation by the Cbl E3 ubiquitin ligase
    Yuan Shao
    Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    EMBO Rep 4:425-31. 2003
    ..Previous studies have proposed that Cbl is also a positive regulator of CrkL-C3G signalling, which leads to Rap1 activation...
  22. ncbi Negative regulation of T cell antigen receptor-mediated Crk-L-C3G signaling and cell adhesion by Cbl-b
    Wenying Zhang
    La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 278:23978-83. 2003
    ..Thus, Cbl-b plays a negative role in Crk-L-C3G-mediated Rap1 and LFA-1 activation in T cells...
  23. ncbi Dynamin 2 regulates T cell activation by controlling actin polymerization at the immunological synapse
    Timothy S Gomez
    Department of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Nat Immunol 6:261-70. 2005
    ..These data identify a functionally important interaction between Dyn2 and Vav1 that regulates actin reorganization and multiple signaling pathways in T lymphocytes...
  24. ncbi The C-terminal SH3 domain of CRKL as a dynamic dimerization module transiently exposing a nuclear export signal
    Maria Harkiolaki
    Cancer Research UK Cell Signalling Group, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
    Structure 14:1741-53. 2006
    b>CRKL plays essential roles in cell signaling. It consists of an N-terminal SH2 domain followed by two SH3 domains. SH2 and SH3N bind to signaling proteins, but the function of the SH3C domain has remained largely enigmatic...
  25. pmc Amplification of CRKL induces transformation and epidermal growth factor receptor inhibitor resistance in human non-small cell lung cancers
    Hihu Wing Cheung
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Cancer Discov 1:608-25. 2011
    ..21 in a subset of primary lung adenocarcinomas. Here we show that CRKL, encoding for an adaptor protein, is amplified and overexpressed in non-small cell lung cancer (NSCLC) cells that ..
  26. doi Overexpression of CRKL correlates with malignant cell proliferation in breast cancer
    Tingting Zhao
    Department of Surgical Oncology and General Surgery, First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China
    Tumour Biol 34:2891-7. 2013
    Crk-like (CRKL) is an adapter protein that has crucial roles in multiple biological processes, including cell proliferation, adhesion, and migration...
  27. pmc Src-inducible association of CrkL with procaspase-8 promotes cell migration
    Ryon Graf
    Department of Reproductive Medicine Division of Gynecologic Oncology University of California San Diego School of Medicine La Jolla, CA USA The UCSD Moores Cancer Center La Jolla, CA USA Graduate School of Biomedical Sciences, Sanford Burnham Medical Research Institute, La Jolla, CA USA
    Cell Adh Migr 7:362-9. 2013
    ..Here we show that the cytoskeletal adaptor protein CrkL promotes caspase-8 recruitment to the peripheral spreading edge of cells, and that the catalytic domain of caspase-..
  28. ncbi p130CAS forms a signaling complex with the adapter protein CRKL in hematopoietic cells transformed by the BCR/ABL oncogene
    R Salgia
    Division of Hematologic Malignancies, Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 271:25198-203. 1996
    ..In primary samples from virtually all patients with CML or Ph+ALL, the CRKL adapter protein is tyrosine phosphorylated and physically associated with p210(BCR/ABL)...
  29. ncbi Direct binding of CRKL to BCR-ABL is not required for BCR-ABL transformation
    C Heaney
    Division of Hematology and Medical Oncology, Oregon Health Sciences University, Portland 97201, USA
    Blood 89:297-306. 1997
    b>CRKL has previously been shown to be a major tyrosine phosphorylated protein in neutrophils of patients with BCR-ABL+ chronic myelogenous leukemia and in cell lines expressing BCR-ABL CRKL and BCR-ABL form a complex as demonstrated by ..
  30. ncbi Interactions of CBL with BCR-ABL and CRKL in BCR-ABL-transformed myeloid cells
    A Bhat
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Biol Chem 272:16170-5. 1997
    ..Since CRKL, an SH2, SH3 domain-containing adapter protein is known to bind directly to BCR-ABL and also binds to tyrosine-..
  31. ncbi Isolation and chromosomal localization of CRKL, a human crk-like gene
    J ten Hoeve
    Department of Pathology, Childrens Hospital, Los Angeles, California 90027
    Oncogene 8:2469-74. 1993
    ..We identified one SH2 and two SH3 domains within the 303 amino acid open reading frame of this crk-like gene, CRKL. The CRKL gene product is predicted to have a molecular mass of 36 kDa...
  32. ncbi B cell antigen receptor signaling induces the formation of complexes containing the Crk adapter proteins
    R J Ingham
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 271:32306-14. 1996
    ..In RAMOS cells, we found that the SH3 domains of Crk-L and Crk II bound C3G. Since C3G activates Rap, a negative regulator of the Ras pathway, Crk proteins may participate in regulation of Ras signaling by the BCR...
  33. ncbi Differential interaction of Crkl with Cbl or C3G, Hef-1, and gamma subunit immunoreceptor tyrosine-based activation motif in signaling of myeloid high affinity Fc receptor for IgG (Fc gamma RI)
    W T Kyono
    Division of Hematology Oncology, Children s Hospital Los Angeles Research Institute, University of Southern California School of Medicine 90027, USA
    J Immunol 161:5555-63. 1998
    Cbl-Crkl and Crkl-C3G interactions have been implicated in T cell and B cell receptor signaling and in the regulation of the small GTPase, Rap1...
  34. pmc Interaction of hematopoietic progenitor kinase 1 with adapter proteins Crk and CrkL leads to synergistic activation of c-Jun N-terminal kinase
    P Ling
    Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 19:1359-68. 1999
    ..We found that HPK1 interacted with Crk and CrkL adaptor proteins in vitro and in vivo and that the proline-rich motifs within HPK1 were involved in the ..
  35. ncbi Signaling pathways activated by interferons
    L C Platanias
    Department of Medicine, The University of Illinois at Chicago and West Side VA Hospital, 60607 7173, USA
    Exp Hematol 27:1583-92. 1999
    ..a pathway involving the vav proto-oncogene product, and a pathway involving adaptor proteins of the Crk-family (CrkL and CrkII)...
  36. doi Capture of an activated receptor complex from the surface of live cells by affinity receptor chromatography
    Andy Jankowski
    Department of Chemistry and Biology, Faculty of Engineering, Architecture, and Science, Ryerson University, Toronto, Ont, Canada
    Anal Biochem 380:235-48. 2008
    ..RHOG (RAS homologue member G), p115 RhoGEF (protein of 115-kDa RAS homologue guanine exchange factor), and CRKL (CRK-like)...
  37. ncbi Regulation of the Erk2-Elk1 signaling pathway and megakaryocytic differentiation of Bcr-Abl(+) K562 leukemic cells by Gab2
    Jay F Dorsey
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Blood 99:1388-97. 2002
    ..activation of the c-fos serum response element (SRE) promoter, whereas overexpression of SHP2, Grb2, and CrkL had no effect...
  38. ncbi L-selectin tyrosine phosphorylates cbl and induces association of tyrosine-phosphorylated cbl with crkl and grb2
    B Brenner
    Department of Pediatrics, Division of Neonatology, Heidelberg University, Im Neuenheimer Feld 150, Heidelberg, 69120, Germany
    Biochem Biophys Res Commun 282:41-7. 2001
    ..There is an activation induced association of tyrosine phosphorylated Cbl with Grb2 and CrkL, respectively, but not CrkII...
  39. doi Inhibition of MDR1 does not sensitize primitive chronic myeloid leukemia CD34+ cells to imatinib
    Sophia Hatziieremia
    Section of Experimental Haematology, Division of Cancer Sciences and Molecular Pathology, Paul O Gorman Leukaemia Research Centre, Gartnavel General Hospital, Faculty of Medicine, University of Glasgow, Glasgow, UK
    Exp Hematol 37:692-700. 2009
    ....
  40. pmc PTPROt inactivates the oncogenic fusion protein BCR/ABL and suppresses transformation of K562 cells
    Tasneem Motiwala
    Department of Molecular and Cellular Biochemistry, College of Medicine, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 284:455-64. 2009
    ..line K562 indeed resulted in hypophosphorylation of bcr/abl and reduced phosphorylation of its downstream targets CrkL and Stat5, confirming that PTPROt could inactivate the function of bcr/abl...
  41. ncbi [Effect on bcr-abl signaling pathway and the mechanisms of apoptosis induction by meisoindigo in K562 cells]
    Bing Cheng Liu
    Institute of Hematology and Blood Diseases Hospital, CAMS, The State Key Laboratory of Experimental Hematology, Tianjin 300020, China
    Zhonghua Xue Ye Xue Za Zhi 29:815-8. 2008
    ..To investigate the effect of meisoindigo on bcr-abl signaling pathway and to explore the mechanism of meisoindigo inducing apoptosis in K562 cells...
  42. ncbi In vitro sensitivity to imatinib-induced inhibition of ABL kinase activity is predictive of molecular response in patients with de novo CML
    Deborah White
    Division of Haematology, Institute of Medical and Veterinary Science IMVS and Hanson Institute, Adelaide, Australia
    Blood 106:2520-6. 2005
    ..samples by measuring the in vitro imatinib-induced reduction of the phosphorylated form of the adaptor protein Crkl (CT10 regulator of kinase like). There was marked variability between patients, with IC50imatinib ranging from 0...
  43. ncbi Impaired Crkl expression contributes to the defective DNA binding of Stat5b in nonobese diabetic mice
    Malini Laloraya
    Center for Biotechnology and Genomic Medicine, Medical College of Georgia, 1120 15th St, Augusta, GA 30912, USA
    Diabetes 55:734-41. 2006
    ..or absence of the Stat5b mutation in these congenic mice but is correlated with the expression levels of the Crkl protein, which was coprecipitated by an anti-Stat5b antibody...
  44. ncbi Intermittent dosage of imatinib mesylate in CML patients with a history of significant hematologic toxicity after standard dosing
    Edgar Faber
    Department of Hemato Oncology, University Hospital Olomouc and Faculty of Medicine, Palacky University in Olomouc, Olomouc, Czech Republic
    Leuk Lymphoma 47:1082-90. 2006
    ..The sensitivity of Bcr/Abl kinase to inhibition by IM was proved in seven patients tested by Crkl phosphorylation assay...
  45. ncbi The multikinase inhibitor sorafenib induces apoptosis in highly imatinib mesylate-resistant bcr/abl+ human leukemia cells in association with signal transducer and activator of transcription 5 inhibition and myeloid cell leukemia-1 down-regulation
    Mohamed Rahmani
    Department of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA
    Mol Pharmacol 72:788-95. 2007
    ..and reporter activity but only very modest and delayed inactivation of the Bcr/Abl downstream target Crkl. Moreover, transfection with a constitutively active STAT5 construct partially but significantly protected cells ..
  46. pmc Downstream signalling and specific inhibition of c-MET/HGF pathway in small cell lung cancer: implications for tumour invasion
    P C Ma
    Division of Hematology Oncology, Department of Medicine, University Hospitals of Case Medical Center and Ireland Cancer Center, Case Western Reserve University, Case Comprehensive Cancer Center, Cleveland, OH 44106, USA
    Br J Cancer 97:368-77. 2007
    ..phosphoepitopes pY1230/1234/1235 (catalytic tyrosine kinase), pY1003 (juxtamembrane), and also of paxillin at pY31 (CRKL-binding site)...
  47. pmc Effective dasatinib uptake may occur without human organic cation transporter 1 (hOCT1): implications for the treatment of imatinib-resistant chronic myeloid leukemia
    Athina Giannoudis
    Department of Haematology, University of Liverpool, Liverpool, United Kingdom
    Blood 112:3348-54. 2008
    ..Dasa-tinib decreased the level of phosphorylated CrkL to 49.9% in control and 40.3% in high hOCT1-expressing cells...
  48. ncbi Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome
    Deborah L Guris
    The Ben May Institute for Cancer Research and Center for Molecular Oncology, The University of Chicago, Chicago, Illinois 60637, USA
    Dev Cell 10:81-92. 2006
    ..Here, we report that compound heterozygosity of mouse homologs of two 22q11 genes, CRKL and TBX1, results in a striking increase in the penetrance and expressivity of a DGS-like phenotype compared to ..
  49. ncbi SET and MYND domain-containing protein 3 decreases sensitivity to dexamethasone and stimulates cell adhesion and migration in NIH3T3 cells
    Xue Gang Luo
    School of Life Science and Technology, China Pharmaceutical University, Tong Jia Xiang 24, Nanjing, Jiangsu, PR China
    J Biosci Bioeng 103:444-50. 2007
    ..downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, ..
  50. ncbi Dok protein family members are involved in signaling mediated by the type 1 Fcepsilon receptor
    Jakub Abramson
    Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel
    Eur J Immunol 33:85-91. 2003
    ..clustering causes both Dok-1 and Dok-2 to become docking sites for other signaling molecules including Nck, CrkL and Cas...
  51. pmc Inactivation of TGFbeta signaling in neural crest stem cells leads to multiple defects reminiscent of DiGeorge syndrome
    Heiko Wurdak
    Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH Honggerberg, Zurich, CH 8093, Switzerland
    Genes Dev 19:530-5. 2005
    ..Moreover, in neural crest cells, TGFbeta signaling is both sufficient and required for phosphorylation of CrkL, a signal adaptor protein implicated in the development of DiGeorge syndrome...
  52. doi A new twist to adaptor proteins contributes to regulation of lymphocyte cell signaling
    Noah Isakov
    The Shraga Segal Department of Microbiology and Immunology, Faculty of Health Sciences and the Cancer Research Center, Ben Gurion University of the Negev, Beer Sheva 84105, Israel
    Trends Immunol 29:388-96. 2008
    ..One family of adaptor molecules includes the CrkII/CrkL proteins that are also involved in the regulation of lymphocyte function...
  53. ncbi CrkL mediates Ras-dependent activation of the Raf/ERK pathway through the guanine nucleotide exchange factor C3G in hematopoietic cells stimulated with erythropoietin or interleukin-3
    Y Nosaka
    First Department of Internal Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113, Japan
    J Biol Chem 274:30154-62. 1999
    b>CrkL is an SH2 and SH3 domain-containing adaptor protein implicated in pathogenesis of chronic myelogenous leukemia...
  54. ncbi T cell development and function in CrkL-deficient mice
    Amy C Peterson
    Department of Medicine, Section of Hematology Oncology, University of Chicago, Chicago, IL 60637, USA
    Eur J Immunol 33:2687-95. 2003
    The adapter protein CrkL has been implicated in multiple signal transduction pathways in hematopoietic cells...
  55. ncbi SHIP1, an SH2 domain containing polyinositol-5-phosphatase, regulates migration through two critical tyrosine residues and forms a novel signaling complex with DOK1 and CRKL
    M Sattler
    Dana Farber Cancer Institute, Department of Adult Oncology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:2451-8. 2001
    ..a novel signaling complex with BCR/ABL that includes DOK1 (p62(DOK)), phosphatidylinositol 3-kinase (PI3K), and CRKL, each of which has been previously shown to regulate migration in diverse cell types...
  56. ncbi BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl
    Bianca Hemmeryckx
    Section of Molecular Carcinogenesis, Division of Hematology Oncology, MS 54, Childrens Hospital of Los Angeles Research Institute, and the Keck School of Medicine University of Southern California, Los Angeles, CA 90027, USA
    Oncogene 21:3225-31. 2002
    ..Multiple independent studies have implicated Crkl, a small adapter protein, in transduction of oncogenic signals of Bcr/Abl and Crkl tyrosine-phosphorylation is used ..
  57. pmc Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes
    Anne M Moon
    Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Dev Cell 10:71-80. 2006
    ..21 disrupt pharyngeal and cardiac development and cause DiGeorge and related human syndromes. CRKL (CRK-Like) lies within 22q11.21, and Crkl-/- mice have phenotypic features of 22q11 deletion (del22q11) syndromes...
  58. ncbi Migration of sympathetic preganglionic neurons in the spinal cord is regulated by Reelin-dependent Dab1 tyrosine phosphorylation and CrkL
    Yee Ping Yip
    Department of Neurobiology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Comp Neurol 502:635-43. 2007
    ..Biochemical studies show that after phosphorylation Dab1 binds to an adaptor protein, CrkL. Whether CrkL is important for Reelin signaling in vivo is unknown, because crkl(-/-) embryos die before cortical ..
  59. pmc A BCR-ABL mutant lacking direct binding sites for the GRB2, CBL and CRKL adapter proteins fails to induce leukemia in mice
    Kara J Johnson
    Division of Hematology and Medical Oncology, Oregon Health and Science University Knight Cancer Institute, Portland, Oregon, United States of America
    PLoS ONE 4:e7439. 2009
    ..Proteins known to be associated with BCR-ABL in this complex include: GRB2, c-CBL, p62(DOK), and CRKL. These proteins in turn, link BCR-ABL to various signaling pathways indicated in cellular transformation...
  60. doi CRK adaptor protein expression is required for efficient replication of avian influenza A viruses and controls JNK-mediated apoptotic responses
    Eike R Hrincius
    Institute of Molecular Virology, ZMBE, Muenster, Germany
    Cell Microbiol 12:831-43. 2010
    ..motif at aa212-217 [PPLPPK] of A/NS1 was shown to be essential for binding to the cellular adaptor proteins CRK and CRKL. Both regulate diverse cellular effector pathways, including activation of the MAP-kinase JNK that in turn mediates ..
  61. doi Interaction between AR signalling and CRKL bypasses casodex inhibition in prostate cancer
    Vikash Reebye
    Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom
    Cell Signal 22:1874-81. 2010
    ..We report a novel cooperative interaction between CRKL (an intracellular signalling adaptor protein) and the AR...
  62. ncbi Crk family adaptors-signalling complex formation and biological roles
    S M Feller
    Cell Signalling Laboratory, Imperial Cancer Research Fund, University of Oxford, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK
    Oncogene 20:6348-71. 2001
    ..Selective formation of multi-protein complexes by the Crk and Crk-like (CRKL) proteins depends on specific motifs recognized by their SH2 and SH3 domains...
  63. ncbi Role for adapter proteins in costimulatory signals of CD2 and IL-2 on NK cell activation
    Hisanori Umehara
    Department of Internal Medicine, Osaka Dental University, Hirakata, Osaka 573 1121, Japan
    Mol Immunol 38:587-96. 2002
    ..3 cells. CD2 crosslinking markedly induced tyrosine phosphorylation of Cbl associated with Grb2 or CrkL, Shc and LAT, compared with IL-2 stimulation...
  64. ncbi Dasatinib (BMS-354825) pharmacokinetics and pharmacodynamic biomarkers in animal models predict optimal clinical exposure
    Feng R Luo
    Pharmaceutical Research Institute, Bristol Myers Squibb Company, Princeton, New Jersey 08543, USA
    Clin Cancer Res 12:7180-6. 2006
    ..To date, dasatinib has shown promising anti-leukemic activity in preclinical models of CML and in phase I/II clinical studies in patients with imatinib-resistant or imatinib-intolerant disease...
  65. pmc Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis
    Tohru Matsuki
    Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA
    J Cell Sci 121:1869-75. 2008
    ..Retrovirally expressed inhibitory RNAs used to reduce Crk and CrkL expression did not block BDNF-enhanced dendritogenesis or influence axonogenesis...
  66. pmc The level of BCR-ABL1 kinase activity before treatment does not identify chronic myeloid leukemia patients who fail to achieve a complete cytogenetic response on imatinib
    Jamshid Sorouri Khorashad
    Department of Haematology, Hammersmith Hospital, Imperial College London, London, United Kingdom
    Haematologica 94:861-4. 2009
    ..and can be investigated by measuring the autophosphorylation levels of BCR-ABL1 or of a surrogate target such as Crkl. In this study we used flow cytometry to investigate the in vitro inhibition of Crkl phosphorylation by imatinib in ..
  67. ncbi A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1
    D Ensenat
    Department of Microbiology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 274:33945-50. 1999
    ..its proline-rich domains, with growth factor receptor-bound 2 (Grb2), CT10-regulated kinase (Crk), and Crk-like (CrkL) adaptor proteins...
  68. ncbi Thrombopoietin induces the generation of distinct Stat1, Stat3, Stat5a and Stat5b homo- and heterodimeric complexes with different kinetics in human platelets
    H Schulze
    Department of Pediatric Hematology and Oncology, Medical School Hannover, Hannover, Germany
    Exp Hematol 28:294-304. 2000
    ..Our aim was to elucidate which Stat-complexes are formed in TPO signal transduction in human blood platelets...
  69. ncbi Crkl enhances leukemogenesis in BCR/ABL P190 transgenic mice
    B Hemmeryckx
    Department of Hematology Oncology, Childrens Hospital Los Angeles Research Institute and the Keck School of Medicine of the University of Southern California, 90027, USA
    Cancer Res 61:1398-405. 2001
    The adapter protein Crkl has been implicated in the abnormal signal transduction pathways activated by the Bcr/Abl oncoprotein, which causes Philadelphia-positive leukemias in humans...
  70. ncbi Platelet activation via the collagen receptor GPVI is not altered in platelets from chronic myeloid leukaemia patients despite the presence of the constitutively phosphorylated adapter protein CrkL
    D Best
    Department of Pharmacology, University of Oxford, Mansfield, Oxford OX1 3QT, UK
    Br J Haematol 112:609-15. 2001
    In this study, we show that the adapter proteins CrkL and Cbl undergo increases in tyrosine phosphorylation and form an intracellular complex in platelets stimulated with the snake venom toxin convulxin, a selective agonist at the ..
  71. ncbi Role of Stat5 in type I interferon-signaling and transcriptional regulation
    Shahab Uddin
    Section of Hematology Oncology, University of Chicago, Chicago, IL 60637, USA
    Biochem Biophys Res Commun 308:325-30. 2003
    ..In previous studies, we have shown that Stat5 associates with the CrkL adapter and forms a signaling complex that binds DNA...
  72. ncbi Microarray analysis of the Df1 mouse model of the 22q11 deletion syndrome
    Katrina Prescott
    Molecular Medicine Unit, Institute of Child Health, 30 Guilford St, London, WC1N 1EH, UK
    Hum Genet 116:486-96. 2005
    ..However Crkl, another gene implicated in the 22q11DS phenotype, was found to be downregulated by microarray and RTQPCR.
  73. ncbi Active (p)CrkL is overexpressed in human malignancies: potential role as a surrogate parameter for therapeutic tyrosine kinase inhibition
    Christian F Singer
    Division of Senology, University of Vienna, Vienna, Austria
    Oncol Rep 15:353-9. 2006
    b>CrkL is a nuclear adaptor and transcriptional activator in Bcr-Abl expressing cells and constitutes the major tyrosine phosphorylated protein in CML, but the expression and biological function of CrkL in other malignancies is largely ..
  74. ncbi Gene expression of AGS cells stimulated with released proteins by Helicobacter pylori
    Nayoung Kim
    Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi Do, Korea
    J Gastroenterol Hepatol 23:643-51. 2008
    ..The present study was performed to evaluate the gene expression of AGS gastric cancer cells stimulated with released proteins by H. pylori...
  75. pmc Transforming growth factor-beta signaling and ubiquitinators in cancer
    Eric Glasgow
    Laboratory of Cancer Genetics, Digestive Diseases, and GI Developmental Biology, Department of Surgery, Medicine and Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA
    Endocr Relat Cancer 15:59-72. 2008
    ..on modulation by adaptor proteins such as embryonic liver fodrin, Smad anchor for receptor activation, filamin, and crkl. A further layer of regulation is imposed by ubiquitin-mediated targeting and proteasomal degradation of specific ..
  76. pmc Crk and CrkL adaptor proteins: networks for physiological and pathological signaling
    Raymond B Birge
    Department of Biochemistry and Molecular Biology, UMDNJ New Jersey Medical School, 185 South Orange Ave, Newark, NJ 07103, USA
    Cell Commun Signal 7:13. 2009
    The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling...
  77. ncbi [Up-regulation of Stathmin and CrkL protein expressions in adriamycin-resistant leukemia cell line K562/A02]
    Jian ping Xu
    Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China
    Zhongguo Shi Yan Xue Ye Xue Za Zhi 19:1383-7. 2011
    ..Two up-regulated proteins Stathmin and CrkL were selected for verification in K562 and K562/A02 cells...
  78. pmc Crk adaptor proteins act as key signaling integrators for breast tumorigenesis
    Kelly E Fathers
    Department of Biochemistry, McGill University, Rosalind and Morris Goodman Cancer Research Centre, 3655 Promenade Sir William Osler, Montreal, QC H3G 1Y6, Canada
    Breast Cancer Res 14:R74. 2012
    CT10 regulator of kinase (Crk) adaptor proteins (CrkI, CrkII and CrkL) play a role in integrating signals for migration and invasion of highly malignant breast cancer cell lines...
  79. pmc Identification of novel candidate gene loci and increased sex chromosome aneuploidy among infants with conotruncal heart defects
    Kazutoyo Osoegawa
    Center for Genetics, Children s Hospital Oakland Research Institute, Children s Hospital Research Center Oakland, Oakland, California
    Am J Med Genet A 164:397-406. 2014
    ..From these duplications and deletions, we found five high priority candidate genes (GATA4, CRKL, BMPR1A, SNAI2, and ZFHX4)...
  80. pmc Curcumin derivative C817 inhibits proliferation of imatinib-resistant chronic myeloid leukemia cells with wild-type or mutant Bcr-Abl in vitro
    Li xian Wu
    1 Department of Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China 2 Institute of Materia Medica, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China 3 Fuijan Key Laboratory of Natural Medicine Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China
    Acta Pharmacol Sin 35:401-9. 2014
    ....
  81. pmc Integrative analysis of high-throughput RNAi screen data identifies the FER and CRKL tyrosine kinases as new regulators of the mitogenic ERK-dependent pathways in transformed cells
    Philippe Nizard
    INSERM UMR1085, Institut de Recherche sur la Santé l Environnement et le Travail IRSET, Universite Rennes 1, SFR Biosit, Rennes, France
    BMC Genomics 15:1169. 2014
    ..Here, we propose a small interfering RNA genetic screen and an integrative approach to identify kinase networks involved in the proliferation of cancer cells...
  82. doi CRKL knockdown promotes in vitro proliferation, migration and invasion, in vivo tumor malignancy and lymph node metastasis of murine hepatocarcinoma Hca-P cells
    Ji Shi
    Department of Biochemistry, Dalian Medical University, Dalian 116044, China Department of Pathology, The First Affiliated Hospital of China Medical University, Anshan Hospital, Liaoning 114012, China
    Biomed Pharmacother 71:84-90. 2015
    Our previous study (Biomed Pharmacother 2015;69:11) demonstrated that the over-expression of CRKL, a chicken tumor virus number 10 regulator of kinase-like protein, suppresses in vitro proliferation, invasion and migration of murine ..
  83. doi CrkL meditates CCL20/CCR6-induced EMT in gastric cancer
    Gang Han
    Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, Shandong, China
    Cytokine 76:163-9. 2015
    In recent years, Crk-like adapter protein (CrkL) has been identified as a key regulator in the epithelial-to-mesenchymal transition (EMT)...
  84. doi Hepatic miR-126 is a potential plasma biomarker for detection of hepatitis B virus infected hepatocellular carcinoma
    Amit Ghosh
    Department of Hepatology and School of Digestive and Liver Diseases, Centre for Liver Research, Institute of Post Graduate Medical Education and Research, Kolkata, India
    Int J Cancer 138:2732-44. 2016
    ..of these two miRNAs in hepato-carcinogenesis, target genes related to cancer pathways (APAF1, APC2, CDKN2A, IRS1, CRKL, LIFR, EGR2) were verified...
  85. pmc CRKL mediates EML4-ALK signaling and is a potential therapeutic target for ALK-rearranged lung adenocarcinoma
    Rong An
    Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Oncotarget 7:29199-210. 2016
    ..We identified CRKL as a novel downstream effector of ALK signaling...
  86. pmc Fibroblast Growth Requires CT10 Regulator of Kinase (Crk) and Crk-like (CrkL)
    Taeju Park
    From the Children s Research Institute, Children s Mercy Kansas City, Kansas City, Missouri 64108
    J Biol Chem 291:26273-26290. 2016
    CT10 regulator of kinase (Crk) and Crk-like (CrkL) are the cellular counterparts of the viral oncogene v-Crk Elevated levels of Crk and CrkL have been observed in many human cancers; inhibition of Crk and CrkL expression reduced the tumor-..
  87. ncbi Tyrosine phosphorylation of murine Crkl
    R De Jong
    Department of Pathology, Childrens Hospital of Los Angeles, California 90027, USA
    Oncogene 11:1469-74. 1995
    The SH2/SH3 adaptor protein Crkl is abnormally phosphorylated on tyrosine by the Bcr/Abl protein in leukemic cells from patients with Philadelphia-chromosome (Ph)positive leukemia...
  88. ncbi Erythropoietin and interleukin-3 activate tyrosine phosphorylation of CBL and association with CRK adaptor proteins
    D L Barber
    Ontario Cancer Institute, Division of Cellular and Molecular Biology, Toronto, Canada
    Blood 89:3166-74. 1997
    ..Recent studies have shown that the CrkL adaptor protein and the Cbl protooncoprotein are constitutively tyrosine phosphorylated and form a preformed ..
  89. ncbi Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome
    D L Guris
    The Ben May Institute for Cancer Research and Center for Molecular Oncology, The University of Chicago, Chicago, Illinois, USA
    Nat Genet 27:293-8. 2001
    ..b>CRKL maps within the common deletion region for DGS/VCFS (ref...
  90. ncbi c-Cbl facilitates fibronectin matrix production by v-Abl-transformed NIH3T3 cells via activation of small GTPases
    A M Teckchandani
    Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania, PA 19140, USA
    Oncogene 20:1739-55. 2001
    ..The observed activation of these GTPases is mediated by the recruitment of phosphatidylinositol-3' kinase, CrkL and Vav2 to the C-terminal tyrosine residues of c-Cbl...
  91. ncbi Effects of the tyrosine kinase inhibitor imatinib mesylate on a Bcr-Abl-positive cell line: suppression of autonomous cell growth but no effect on decreased adhesive property and morphological changes
    Toshio Nishihara
    Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Int J Hematol 78:233-40. 2003
    ..Imatinib mesylate remarkably reduced tyrosine phosphorylation of Bcr-Abl, Cbl, and Crkl in a time-dependent manner, and their complex formation also was affected...
  92. ncbi OCT-1-mediated influx is a key determinant of the intracellular uptake of imatinib but not nilotinib (AMN107): reduced OCT-1 activity is the cause of low in vitro sensitivity to imatinib
    Deborah L White
    Division of Hematology, Institute of Medical and Veterinary Science IMVS and Hanson Institute, Adelaide, South Australia
    Blood 108:697-704. 2006
    ..is defined as the in vitro concentration of drug required to reduce phosphorylation of the adaptor protein Crkl by 50%...
  93. ncbi A noncanonical SH3 domain binding motif links BK channels to the actin cytoskeleton via the SH3 adapter cortactin
    Lijun Tian
    Centre for Integrative Physiology, School of Biomedical Science, University of Edinburgh, Edinburgh EH8 9XD, Scotland, UK
    FASEB J 20:2588-90. 2006
    ..Targeting of cortactin to BK channels via a novel, noncanonical SH3 domain binding motif has important implications for the coordination of BK channel function in normal physiology and disease...
  94. ncbi Nilotinib exerts equipotent antiproliferative effects to imatinib and does not induce apoptosis in CD34+ CML cells
    Heather G Jørgensen
    Section of Experimental Haematology, Division of Cancer Sciences and Molecular Pathology, University of Glasgow, 10 Alexandra Parade, Glasgow, UK
    Blood 109:4016-9. 2007
    ..and IM were equipotent for inhibition of BcrAbl activity, producing equivalent but incomplete reduction in CrkL phosphorylation at 5 microM...
  95. ncbi A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion
    Catherine A Moore
    MRC Centre for Developmental and Biomedical Genetics, Department of Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Development 134:3145-53. 2007
    ..In addition, we show that the adaptor proteins Crk and Crk-like (Crkl), with which Dock proteins are known to interact physically, are also required for myoblast fusion.
  96. ncbi Measurement of in vivo BCR-ABL kinase inhibition to monitor imatinib-induced target blockade and predict response in chronic myeloid leukemia
    Deborah White
    Division of Hematology, Institute of Medical and Veterinary Science, Adelaide, Australia
    J Clin Oncol 25:4445-51. 2007
    ..This suggests that patient-tailored dosing may be more rational than a fixed dose for all. Dose optimization potentially could be based on accurate measurement of the level of BCR-ABL kinase inhibition achieved in vivo...
  97. doi Activity of tyrosine kinase inhibitors against human NUP214-ABL1-positive T cell malignancies
    A Quintas-Cardama
    Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Leukemia 22:1117-24. 2008
    ..of cellular proliferation was associated with time-dependent induction of apoptosis and inhibition of ABL, CrKL and STAT5 phosphorylation...
  98. pmc PI3K links NKG2D signaling to a CrkL pathway involved in natural killer cell adhesion, polarity, and granule secretion
    Colin M Segovis
    Department of Immunology, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Immunol 182:6933-42. 2009
    ..We demonstrate that an interaction between the regulatory subunit of PI3K, p85, and the adaptor protein CrkL is required for efficient NKG2D-mediated cellular cytotoxicity...
  99. pmc CrkII transgene induces atypical mammary gland development and tumorigenesis
    Kelly E Fathers
    Departments of Biochemistry, Rosalind and Morris Goodman Cancer Centre, McGill University, Montreal, QC H3A 1A3, Canada
    Am J Pathol 176:446-60. 2010
    ..Cellular homologues of v-Crk include Crk, which encodes two alternatively spliced proteins (CrkI and CrkII), and CrkL. Though CrkI/II proteins are elevated in several types of cancer, including breast, the question of whether these ..
  100. pmc Mutations in the NS1 C-terminal tail do not enhance replication or virulence of the 2009 pandemic H1N1 influenza A virus
    Benjamin G Hale
    Department of Microbiology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, NY 10029, USA
    J Gen Virol 91:1737-42. 2010
    ..as compared with the parental 1918 virus, most notably a K217E substitution that abolishes binding to host Crk/CrkL signalling adapters, and an 11 aa C-terminal truncation...
  101. pmc A comprehensive molecular interaction map for rheumatoid arthritis
    Gang Wu
    Group of Clinical Genomic Networks, CAS MPG Partner Institute for Computational Biology, Chinese Academy of Sciences, Shanghai, People s Republic of China
    PLoS ONE 5:e10137. 2010
    ..In this study, we use computation methods in order to improve our understanding of the complex interactions that occur between molecules related to Rheumatoid Arthritis (RA)...

Research Grants24

  1. CLONING OF THE Hol MUTATION
    Licia Selleri; Fiscal Year: 2009
    ..by exploiting high-resolution genetic mapping, we discounted that the Hol mutation maps to either the Tbx1 or Crkl loci on chromosome 16...
  2. Developmental Genetics of the Pharyngeal Apparatus
    Bernice E Morrow; Fiscal Year: 2010
    ..Recently, Crkl, another gene deleted on 22q11.2, was shown to mediate Fgf8 signaling downstream of Tbx1...
  3. Janet C King; Fiscal Year: 2014
    ..Based on a novel microdeletion of the gene CRKL that we found by array-CGH, we will perform Next Generation DNA sequencing to identify mutations of genes of the ..
  4. KERIANN MARIE BACKUS; Fiscal Year: 2014
    ..proteins, including the epigenetic enzyme PRMT1, the transcription factor NF-?B, the oncogenic adaptor protein CRKL, cancer metabolism enzyme IDH1 and the poorly characterized kinase ZAK...
  5. Genetic Basis of Birth Defects in 22q11 Deletion Syndrome
    Bernice E Morrow; Fiscal Year: 2010
    ..For example, a gene in the proximal (centromeric) half of the 3 Mb region, termed TBX1, and distal half, termed CRKL have been found to genetically interact for cardiovascular development...
  6. Jak2 involvement in Bcr-Abl oncogenic transformation
    Ralph Arlinghaus; Fiscal Year: 2005
    ..They include SH2-Bbeta, p56 DOK2, p56 LYN and CrkL; these proteins are tyrosine-phosphorylated as well...
  7. Stat5 dephosphorylation by Shp-2
    Demin Wang; Fiscal Year: 2007
    ..Using the tyrosine-phosphorylated Stat5A peptides, we have co-purified the adapter protein, CrkL, with Shp-2. CrkL is able to associate with both Stat5 and Shp-2 in cells...
  8. Mechanism of RIN1 Signaling in Neuronal Plasticity
    John Colicelli; Fiscal Year: 2007
    ..RIN1 promotes the ABL2 phosphorylation of CRKII and CRKL, adaptor proteins that coordinate cytoskeletal remodeling...
  9. BCR AND ABL RELATED GENES
    John Groffen; Fiscal Year: 1999
    ..They have identified and cloned the adaptor- signaling molecule Crkl as a specific major substrate for the tyrosine kinase activity in Bcr/Abl...
  10. The Role of CbL in AbL Signaling and Leukemia
    Hua Gu; Fiscal Year: 2006
    ..are phosphorylated by c-Abl and associate with c-Abl and other signaling molecules downstream of c-Abl, such as CrkL, p85 of PI-3 kinase and Vav, upon c-Abl activation...
  11. ADAPTOR PROTEINS AND MALIGNANT TRANSFORMATION
    Douglas Tyler; Fiscal Year: 2001
    ..This project will focus on the adaptor protein CRKL which is constitutively expressed in a number of hematopoietic and non-hematopoietic cells in an unphosphorylated ..
  12. CBL FUNCTION IN CELLULAR SIGNALING AND TRANSFORMATION
    Brian Druker; Fiscal Year: 2003
    ..In Abl-transformed cells, tyrosine phosphorylated Cbl associates with the SH2 domains of activated Abl, CrkL, and PI 3-kinase...
  13. CBL FUNCTION IN MYELOID TRANSFORMATION
    Kathryn Kolibaba; Fiscal Year: 2000
    ..tyrosine phosphorylated CBL interacts with a variety of SH2 domain-containing proteins including activated ABL, CRKL, GRB-2, and PI3-kinase...
  14. CHRONIC MYELOGENOUS LEUKEMIA AND BCR-ABL SUBSTRATES
    Brian Druker; Fiscal Year: 2009
    ..In that last aim, we will follow up on our finding that Bcr-Abl is unable to transform CrkL null cells and that CrkL null cells expressing Bcr-Abl have a variety of signaling defects...
  15. Phase I Clinical Trials of Anti-Cancer Agents
    Francis Giles; Fiscal Year: 2007
    ..abstract_text> ..
  16. ASPL TFE3 fusion in human cancers
    Marc Ladanyi; Fiscal Year: 2006
    ..3->qter; and finally analyze selected candidate genes in the same region for inactivating mutations. ..
  17. Targeting FKBP52 and Copper in Alzheimer's Disease
    Raymond Birge; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  18. Role of the JIP-1-p190RhoGEF Complex In Neuron Function
    WILLIAM ARTHUR; Fiscal Year: 2004
    ..Furthermore, the importance of the JIP-l-p190RhoGEF association in brain development and neuronal migration will be investigated by disrupting this complex in mutant mice . ..
  19. IDENTIFICATION OF A NOVEL SIGNAL PATHWAY FOR NGF
    Raymond Birge; Fiscal Year: 2003
    ....
  20. Mutagenesis of Tbx3: a model of ulnar-mammary syndrome
    Anne M Moon; Fiscal Year: 2010
    ..The flexible model system we propose will be a valuable tool for developmental studies of many organs and lead to a deeper understanding of the genetic and molecular bases of congenital anomalies in humans. ..
  21. CONDITIONAL MUTAGENESIS OF FIBROBLAST GROWTH FACTORS
    Anne Moon; Fiscal Year: 2002
    ..Ultimately, this knowledge will help us to understand how alterations in those pathways, whether genetic or environmental, result in congenital malformations in infants and children. ..
  22. Studies of a Novel Therapeutic Target in Non-Small Cell Lung Cancer (NSCLC)
    Ravi Salgia; Fiscal Year: 2011
    ..Conduct an anti-c-Met phase I/I I clinical trial against NSCLC. Through the achievement of the goals proposed in these specific aims, we will arrive at novel therapy against c- Met in lung cancer. ..
  23. SIGNALING THROUGH THE CYTOSKELETAL PROTEIN, PAXILLIN
    Sheila Thomas; Fiscal Year: 2001
    ....
  24. INTEGRATION OF STRESS AND MITOGEN SIGNALING
    Jonathan Cooper; Fiscal Year: 2001
    ..4) To map the interactions between ERK and Msk using approaches such at the two-hybrid system. 5) To clone Mapkip108 using conventional techniques. ..