CENPN

Summary

Gene Symbol: CENPN
Description: centromere protein N
Alias: BM039, C16orf60, CENP-N, ICEN32, centromere protein N, interphase centromere complex protein 32
Species: human

Top Publications

  1. ncbi The human CENP-A centromeric nucleosome-associated complex
    Daniel R Foltz
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093 0670, USA
    Nat Cell Biol 8:458-69. 2006
  2. doi The ABCs of CENPs
    Marinela Perpelescu
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka, Japan
    Chromosoma 120:425-46. 2011
  3. ncbi The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 8:446-57. 2006
  4. ncbi Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells
    Hiroshi Izuta
    Bioscience and Biotechnology Center, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Genes Cells 11:673-84. 2006
  5. pmc CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly
    Sebastian Hoffmann
    Institut Curie, PSL Research University, CNRS, UMR 144, 26 Rue d Ulm, F 75005, Paris, France
    Cell Rep 17:2394-2404. 2016
  6. doi Insights from biochemical reconstitution into the architecture of human kinetochores
    John R Weir
    Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Straße 11, 44227 Dortmund, Germany
    Nature 537:249-253. 2016
  7. pmc A fast and powerful W-test for pairwise epistasis testing
    Maggie Haitian Wang
    Division of Biostatistics and Centre for Clinical Research and Biostatistics, JC School of Public Health and Primary Care, The Chinese University of Hong Kong, Shatin, N T, Hong Kong SAR, China CUHK Shenzhen Research Institute, Shenzhen, China
    Nucleic Acids Res 44:e115. 2016
  8. pmc The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface
    Kara L McKinley
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 60:886-98. 2015
  9. pmc Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore
    Itaru Samejima
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK
    J Cell Biol 211:1141-56. 2015
  10. pmc A supergene determines highly divergent male reproductive morphs in the ruff
    Clemens Küpper
    Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK
    Nat Genet 48:79-83. 2016

Research Grants

  1. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005

Scientific Experts

  • Samantha G Zeitlin
  • Christopher W Carroll
  • Tatsuo Fukagawa
  • Marinela Perpelescu
  • Daniela Hellwig
  • Tetsuya Hori
  • Patrick Meraldi
  • Aaron F Straight
  • Andrew D McAinsh
  • John R Weir
  • Sebastian Hoffmann
  • Clemens Küpper
  • Maggie Haitian Wang
  • Kara L McKinley
  • Junnan Fang
  • Itaru Samejima
  • Sarah A Andres
  • Stephen M Hinshaw
  • Qiong Guo
  • William C Earnshaw
  • Anja Eskat
  • Daniel R Matson
  • Tobias Ulbricht
  • Volker Doring
  • Stephan Diekmann
  • Christian Hoischen
  • Stephan Emmerth
  • Don W Cleveland
  • Iain M Cheeseman
  • Ben E Black
  • Susana Abreu Ribeiro
  • Lars E T Jansen
  • Sarah E McClelland
  • Hiroshi Izuta
  • Daniel R Foltz
  • John R Yates
  • Masahiro Okada
  • Solène Hervé
  • Jack Lee
  • Arsen Petrovic
  • Daniele Fachinetti
  • Kai Zeng
  • Natalie Dos Remedios
  • Judith E Risse
  • John C Wingfield
  • Pak Chung Sham
  • Viviana Barra
  • Peter Ly
  • Rui Sun
  • Lindsay L Farrell
  • Susan B McRae
  • Theunis Piersma
  • David B Lank
  • Yael Nechemia-Arbely
  • Doro Vogt
  • Natalia Karlionova
  • Sabine Wohlgemuth
  • Moira A McMahon
  • Inchi Hu
  • Kerstin Klare
  • Jon Slate
  • Josef Fischböck
  • Dongqing Pan
  • Alex C Faesen
  • Mark Blaxter
  • Marie Dumont
  • Michael Stocks
  • Jenny Keller
  • Andrea Musacchio
  • Benny Chung Ying Zee
  • Federica Basilico
  • Junfeng Guo
  • Terry Burke
  • Pavel Pinchuk
  • Yvonne I Verkuil
  • Franz Herzog
  • Satyakrishna Pentakota
  • Alexander S Kitaysky
  • Haoyi Weng
  • Tawna C Morgan
  • Marion E Pesenti
  • Katie E Bickett
  • Zhouliang Yu
  • Christos Spanos
  • Tonia Tsinman
  • Yuting Liu
  • Haihe Ruan
  • Yan Teng
  • Wenqiang Deng
  • Li Huang

Detail Information

Publications22

  1. ncbi The human CENP-A centromeric nucleosome-associated complex
    Daniel R Foltz
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093 0670, USA
    Nat Cell Biol 8:458-69. 2006
    ..The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling...
  2. doi The ABCs of CENPs
    Marinela Perpelescu
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka, Japan
    Chromosoma 120:425-46. 2011
    ..This review presents discussion of the latest structural and functional data on CENP-A and CENPs from the CCAN as well as their interaction with the KMN network...
  3. ncbi The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 8:446-57. 2006
    ....
  4. ncbi Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells
    Hiroshi Izuta
    Bioscience and Biotechnology Center, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Genes Cells 11:673-84. 2006
    ..These results suggest that the seven components of the ICEN complex are predominantly localized at the centromeres and are required for kinetochore function perhaps through or not through loading of CENP-H and hMis6 onto the centromere...
  5. pmc CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly
    Sebastian Hoffmann
    Institut Curie, PSL Research University, CNRS, UMR 144, 26 Rue d Ulm, F 75005, Paris, France
    Cell Rep 17:2394-2404. 2016
    ..Thus, there is a reciprocal interdependency of CENP-A chromatin and the underlying repetitive centromere DNA sequences bound by CENP-B in the maintenance of human chromosome segregation...
  6. doi Insights from biochemical reconstitution into the architecture of human kinetochores
    John R Weir
    Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto Hahn Straße 11, 44227 Dortmund, Germany
    Nature 537:249-253. 2016
    ..Biochemical reconstitution paves the way for mechanistic and quantitative analyses of kinetochores...
  7. pmc A fast and powerful W-test for pairwise epistasis testing
    Maggie Haitian Wang
    Division of Biostatistics and Centre for Clinical Research and Biostatistics, JC School of Public Health and Primary Care, The Chinese University of Hong Kong, Shatin, N T, Hong Kong SAR, China CUHK Shenzhen Research Institute, Shenzhen, China
    Nucleic Acids Res 44:e115. 2016
    ..GWAS) datasets, the W-test identified significant interactions pairs that can be replicated, including SLIT3-CENPN, SLIT3-TMEM132D, CNTNAP2-NDST4 and CNTCAP2-RTN4R The genes in the pairs play central roles in neurotransmission ..
  8. pmc The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface
    Kara L McKinley
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 60:886-98. 2015
    ..Thus, an interdependent meshwork within the CCAN underlies the centromere specificity and stability of the kinetochore...
  9. pmc Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore
    Itaru Samejima
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK
    J Cell Biol 211:1141-56. 2015
    ..Our data also suggest that CENP-O and CENP-R may regulate the size of the inner kinetochore without influencing the assembly of the outer kinetochore. ..
  10. pmc A supergene determines highly divergent male reproductive morphs in the ruff
    Clemens Küpper
    Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK
    Nat Genet 48:79-83. 2016
    ..One breakpoint of the inversion disrupts the essential CENP-N gene (encoding centromere protein N), and pedigree analysis confirms the lethality of homozygosity for the inversion...
  11. doi Interaction between smoking history and gene expression levels impacts survival of breast cancer patients
    Sarah A Andres
    Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY, 40202, USA
    Breast Cancer Res Treat 152:545-56. 2015
    ..BMC Cancer 13:326, 2013a; Horm Cancer 4:208-221, 2013b), multivariable analyses revealed CENPN, CETN1, CYP1A1, IRF2, LECT2, and NCOA1 to be important predictors for both breast carcinoma recurrence and ..
  12. pmc Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N
    Junnan Fang
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China
    Genes Dev 29:1058-73. 2015
    ..Our results provide the first insights into the mechanisms by which the recruitment of CENP-N is regulated by the structural transitions between compaction and relaxation of centromeric chromatin during the cell cycle. ..
  13. pmc Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex
    Anja Eskat
    Molecular Biology, FLI, Jena, Germany
    PLoS ONE 7:e44717. 2012
    ....
  14. pmc An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation
    Stephen M Hinshaw
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Cell Rep 5:29-36. 2013
    ..We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation. ..
  15. doi Structural insights into the role of the Chl4-Iml3 complex in kinetochore assembly
    Qiong Guo
    Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 69:2412-9. 2013
    ..Acting as a heterodimer, the Chl4-Iml3 complex exhibits a low-affinity nonspecific DNA-binding activity which may play an important role in the kinetochore-assembly process. ..
  16. pmc A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint
    Daniel R Matson
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    Genes Dev 26:542-7. 2012
    ..We propose a model to explain the differential response of human cells to nocodazole and paclitaxel...
  17. pmc Dynamics of CENP-N kinetochore binding during the cell cycle
    Daniela Hellwig
    Molecular Biology, FLI, Beutenbergstrasse 11, 07745 Jena, Germany
    J Cell Sci 124:3871-83. 2011
    ..We propose a model in which CENP-N functions as a fidelity factor during centromeric replication and reveal that the CCAN network is considerably more dynamic than previously appreciated...
  18. pmc Dual recognition of CENP-A nucleosomes is required for centromere assembly
    Christopher W Carroll
    Department of Biochemistry, Stanford University School of Medicine, Palo Alto, CA 94503, USA
    J Cell Biol 189:1143-55. 2010
    ..We propose that CENP-C binds directly to CENP-A chromatin and, together with CENP-N, provides the foundation upon which other centromere and kinetochore proteins are assembled...
  19. pmc A super-resolution map of the vertebrate kinetochore
    Susana Abreu Ribeiro
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Proc Natl Acad Sci U S A 107:10484-9. 2010
    ..In mitosis, a CENP-C-dependent mechanism crosslinks CENP-A blocks of different layers together, conferring extra stability to the kinetochore...
  20. pmc Double-strand DNA breaks recruit the centromeric histone CENP-A
    Samantha G Zeitlin
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 106:15762-7. 2009
    ..Finally, since cell survival after radiation-induced DNA damage correlates with CENP-A expression level, we propose that CENP-A may have a function in DNA repair...
  21. pmc Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N
    Christopher W Carroll
    Department of Biochemistry, Stanford University School of Medicine, Beckman Center, Palo Alto, CA 94503 5307, USA
    Nat Cell Biol 11:896-902. 2009
    ..These data suggest that CENP-N interprets the information encoded within CENP-A nucleosomes and recruits other proteins to centromeric chromatin that are required for centromere function and propagation...
  22. pmc The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity
    Sarah E McClelland
    Chromosome Segregation Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
    EMBO J 26:5033-47. 2007
    ..Based on our results, we propose that the cooperative action of CENP-A NAC/CAD subunits and the KMN network drives efficient chromosome segregation and bipolar spindle assembly during mitosis...

Research Grants1

  1. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005
    ..These studies will also have direct implications for understanding cancer causation, and applications in gene therapy. ..