CENPA

Summary

Gene Symbol: CENPA
Description: centromere protein A
Alias: CENP-A, CenH3, histone H3-like centromeric protein A, centromere autoantigen A, centromere protein A, 17kDa, centromere-specific histone
Species: human

Top Publications

  1. ncbi Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
    A G Uren
    The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, 3050, Victoria, Australia
    Curr Biol 10:1319-28. 2000
  2. ncbi Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated
    Alka Saxena
    Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Rd, Parkville 3052, Australia
    J Biol Chem 277:26921-6. 2002
  3. ncbi Structural determinants for generating centromeric chromatin
    Ben E Black
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA
    Nature 430:578-82. 2004
  4. ncbi Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy
    Takeshi Tomonaga
    Department of Molecular Diagnosis F8, Graduate School of Medicine, Chiba University, Inohana, Chuo Ku, Chiba, Japan
    Cancer Res 65:4683-9. 2005
  5. ncbi Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc
    Alka Saxena
    The Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Road, Parkville 3052, Australia
    Hum Mol Genet 11:2319-29. 2002
  6. pmc Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone
    D K Palmer
    Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    Proc Natl Acad Sci U S A 88:3734-8. 1991
  7. pmc Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere
    K F Sullivan
    Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
    J Cell Biol 127:581-92. 1994
  8. ncbi A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A
    M M Valdivia
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cadiz, Spain
    FEBS Lett 422:5-9. 1998
  9. pmc Autoepitopes on autoantigen centromere protein-A (CENP-A) are restricted to the N-terminal region, which has no homology with histone H3
    Y Muro
    Department of Dermatology, Nagoya University School of Medicine, Nagoya, Japan
    Clin Exp Immunol 120:218-23. 2000
  10. ncbi Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A
    A A Van Hooser
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Cell Sci 114:3529-42. 2001

Research Grants

  1. Molecular Architecture of the Regional Kinetochore in Fission Yeast
    Xiangwei He; Fiscal Year: 2012
  2. Aaron F Straight; Fiscal Year: 2016
  3. Molecular Mechanisms of Chromosome Segregation in Yeast
    Jennifer L Gerton; Fiscal Year: 2012
  4. SUSAN BIGGINS; Fiscal Year: 2015
  5. Don W Cleveland; Fiscal Year: 2016
  6. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
  7. Ben E Black; Fiscal Year: 2016
  8. Donald F Hunt; Fiscal Year: 2016
  9. AUTOIMMUNE ANTIGENS AND SYSTEMIC SCLEROSIS
    SALLIE HOCH; Fiscal Year: 1993
  10. FUNCTIONAL ANALYSIS OF CENTROMERIC CHROMATIN IN YEAST
    Molly Fitzgerald Hayes; Fiscal Year: 2003

Detail Information

Publications180 found, 100 shown here

  1. ncbi Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype
    A G Uren
    The Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, 3050, Victoria, Australia
    Curr Biol 10:1319-28. 2000
    ..To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice...
  2. ncbi Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated
    Alka Saxena
    Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Rd, Parkville 3052, Australia
    J Biol Chem 277:26921-6. 2002
    ..the interaction of this protein with, and poly(ADP-ribosyl)ation of, three constitutive centromere proteins, Cenpa, Cenpb, and Cenpc, and a spindle checkpoint protein, Bub3...
  3. ncbi Structural determinants for generating centromeric chromatin
    Ben E Black
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA
    Nature 430:578-82. 2004
    ..Thus, the centromere-targeting domain of CENP-A confers a unique structural rigidity to the nucleosomes into which it assembles, and is likely to have a role in maintaining centromere identity...
  4. ncbi Centromere protein H is up-regulated in primary human colorectal cancer and its overexpression induces aneuploidy
    Takeshi Tomonaga
    Department of Molecular Diagnosis F8, Graduate School of Medicine, Chiba University, Inohana, Chuo Ku, Chiba, Japan
    Cancer Res 65:4683-9. 2005
    ..These results suggest that the aberrant expression and localization of a kinetochore protein CENP-H plays an important role in the aneuploidy frequently observed in colorectal cancers...
  5. ncbi Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc
    Alka Saxena
    The Murdoch Childrens Research Institute, Royal Children s Hospital, Flemington Road, Parkville 3052, Australia
    Hum Mol Genet 11:2319-29. 2002
    ..assay demonstrates interaction between PARP-2 and its functional homolog PARP-1, constitutive centromere proteins Cenpa and Cenpb, and spindle checkpoint protein Bub3, but not with a third constitutive centromere protein Cenpc...
  6. pmc Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone
    D K Palmer
    Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    Proc Natl Acad Sci U S A 88:3734-8. 1991
    ..Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A...
  7. pmc Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere
    K F Sullivan
    Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
    J Cell Biol 127:581-92. 1994
    ..These data clearly indicate that the assembly of centromeres is driven, at least in part, by the incorporation of a novel core histone into centromeric chromatin...
  8. ncbi A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A
    M M Valdivia
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Cadiz, Spain
    FEBS Lett 422:5-9. 1998
    ..Lastly, we found that in Jurkat cells induced to apoptosis, CENP-A remains associated with the centromere, in contrast to other human autoantigens studied during apoptosis...
  9. pmc Autoepitopes on autoantigen centromere protein-A (CENP-A) are restricted to the N-terminal region, which has no homology with histone H3
    Y Muro
    Department of Dermatology, Nagoya University School of Medicine, Nagoya, Japan
    Clin Exp Immunol 120:218-23. 2000
    ....
  10. ncbi Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A
    A A Van Hooser
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Cell Sci 114:3529-42. 2001
    ....
  11. ncbi CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function
    Naoko Kunitoku
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    Dev Cell 5:853-64. 2003
    ..Our observations suggest that phosphorylation of CENP-A on Ser-7 by Aurora-A in prophase is essential for kinetochore function...
  12. pmc Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin
    Beth A Sullivan
    Molecular and Cell Biology Laboratory, The Salk Institute, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 11:1076-83. 2004
    ..We speculate that this distinct modification pattern contributes to the unique domain organization and three-dimensional structure of centromeric regions, and/or to the epigenetic information that determines centromere identity...
  13. ncbi Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1
    Yohta Fujita
    CREST Research Project of Japan Science and Technology Corporation JST, Graduate School of Biostudies, Kyoto University, Yoshida Honmachi, Kyoto 606 8501, Japan
    Dev Cell 12:17-30. 2007
    ..Telophase centromere chromatin may be primed or licensed by the hMis18 complex and RbAp46/48 to recruit CENP-A through regulating the acetylation status in the centromere...
  14. doi HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres
    Elaine M Dunleavy
    Laboratory of Nuclear Dynamics and Genome Plasticity, UMR CNRS Institut Curie, Paris, France
    Cell 137:485-97. 2009
    The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation...
  15. pmc CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1
    Masahiro Okada
    Center for Priority Areas, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo 192 0397, Japan Innovative Drug Research Laboratories, Kyowa Hakko Kirin Co, Ltd, Takasaki, Gumma, 370 1295, Japan
    Mol Biol Cell 20:3986-95. 2009
    ..These findings indicate that the CENP-H-containing complex facilitates deposition of newly synthesized CENP-A into centromeric chromatin in cooperation with FACT and CHD1...
  16. pmc HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres
    Muhammad Shuaib
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, The Centre National de la Recherche Scientifique, the Institut National de la Santé et de la Recherche Médicale, Universite de Strasbourg, Parc d Innovation, 1 rue Laurent Fries, 67404 Illkirch Cedex, France
    Proc Natl Acad Sci U S A 107:1349-54. 2010
    ..Our data identified HJURP as a vertebrate CENP-A chaperone and dissected its mode of interactions with CENP-A...
  17. pmc CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses
    Kayoko Maehara
    Department of Maternal Fetal Biology, National Center for Child Health and Development, 2 10 1, Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Mol Cell Biol 30:2090-104. 2010
    ....
  18. pmc Dual recognition of CENP-A nucleosomes is required for centromere assembly
    Christopher W Carroll
    Department of Biochemistry, Stanford University School of Medicine, Palo Alto, CA 94503, USA
    J Cell Biol 189:1143-55. 2010
    ..We propose that CENP-C binds directly to CENP-A chromatin and, together with CENP-N, provides the foundation upon which other centromere and kinetochore proteins are assembled...
  19. doi The immunodominant epitope of centromere-associated protein A displays homology with the transcription factor forkhead box E3 (FOXE3)
    Federico Perosa
    Department of Internal Medicine and Clinical Oncology DIMO, University of Bari Medical School, Bari, Italy
    Clin Immunol 137:60-73. 2010
    ..This study sets the ground for future investigations on a possible relationship between antibody specificity and clinical manifestations of systemic sclerosis, as well as on a possible role of FOXE3 in priming these antibodies...
  20. pmc The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres
    Nikolina Sekulic
    Department of Biochemistry and Biophysics, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 6059, USA
    Nature 467:347-51. 2010
    ..Our results indicate that CENP-A marks centromere location by restructuring the nucleosome from within its folded histone core...
  21. pmc ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth
    Yongmei Li
    Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai, China
    PLoS ONE 6:e17794. 2011
    ..Centromere protein A (CENP-A) plays important roles in cell-cycle regulation and genetic stability. Herein, we aimed to investigate its expression pattern, clinical significance, and biological function in hepatocellular carcinoma (HCC)...
  22. doi Crystal structure of the human centromeric nucleosome containing CENP-A
    Hiroaki Tachiwana
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 Wakamatsu cho, Shinjuku ku, Tokyo 162 8480, Japan
    Nature 476:232-5. 2011
    ..The structure provides the first atomic-resolution picture of the centromere-specific nucleosome...
  23. pmc HJURP uses distinct CENP-A surfaces to recognize and to stabilize CENP-A/histone H4 for centromere assembly
    Emily A Bassett
    Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Cell 22:749-62. 2012
    ..These data offer insight into the mechanism by which HJURP discriminates CENP-A from bulk histone complexes and chaperones CENP-A/H4 for a substantial portion of the cell cycle prior to mediating chromatin assembly at the centromere...
  24. pmc Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo
    Minh Bui
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Cell 150:317-26. 2012
    ..Our results reveal a cyclical nature to CENP-A nucleosome structure and have implications for the maintenance of epigenetic memory after centromere replication...
  25. pmc Every amino acid matters: essential contributions of histone variants to mammalian development and disease
    Ian Maze
    1 Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York 10065, USA 2
    Nat Rev Genet 15:259-71. 2014
    ....
  26. pmc Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites
    R D Shelby
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 136:501-13. 1997
    ....
  27. pmc CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis
    S G Zeitlin
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 155:1147-57. 2001
    ..Our data support a model where CENP-A phosphorylation is involved in regulating Aurora B, INCENP, and PP1gamma1 targeting within the cell. These experiments identify an unexpected role for the kinetochore in regulation of cytokinesis...
  28. pmc CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells
    Satoshi Ando
    BioScience Center, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Mol Cell Biol 22:2229-41. 2002
    ..On the basis of these results, we propose that the CENP-A/B/C chromatin complex is selectively formed on the I-type alpha-satellite array and constitutes the prekinetochore in HeLa cells...
  29. ncbi Overexpression and mistargeting of centromere protein-A in human primary colorectal cancer
    Takeshi Tomonaga
    Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba 260 8670, Japan
    Cancer Res 63:3511-6. 2003
    ..These results suggest that overexpression of CENP-A could play an important role for aneuploidy in colorectal cancers...
  30. ncbi The human CENP-A centromeric nucleosome-associated complex
    Daniel R Foltz
    Ludwig Institute for Cancer Research, University of California at San Diego, La Jolla, CA 92093 0670, USA
    Nat Cell Biol 8:458-69. 2006
    ..The CENP-A NAC is essential, as disruption of the complex causes errors of chromosome alignment and segregation that preclude cell survival despite continued centromere-derived mitotic checkpoint signalling...
  31. ncbi The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres
    Masahiro Okada
    Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Nat Cell Biol 8:446-57. 2006
    ....
  32. ncbi Centromere identity maintained by nucleosomes assembled with histone H3 containing the CENP-A targeting domain
    Ben E Black
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell 25:309-22. 2007
    ..These data offer direct support for centromere identity maintained by a unique nucleosome that serves to distinguish the centromere from the rest of the chromosome...
  33. pmc Propagation of centromeric chromatin requires exit from mitosis
    Lars E T Jansen
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 176:795-805. 2007
    ....
  34. pmc Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres
    Alicia Alonso
    Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Genome Biol 8:R148. 2007
    ..Neocentromeres permit detailed investigation of centromeric chromatin organization that is not possible in the highly repetitive alpha satellite DNA present at endogenous centromeres...
  35. pmc The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity
    Sarah E McClelland
    Chromosome Segregation Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey, UK
    EMBO J 26:5033-47. 2007
    ..Based on our results, we propose that the cooperative action of CENP-A NAC/CAD subunits and the KMN network drives efficient chromosome segregation and bipolar spindle assembly during mitosis...
  36. pmc Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP
    Daniel R Foltz
    Ludwig Institute for Cancer Research, La Jolla, CA 92093 0670, USA
    Cell 137:472-84. 2009
    ..We propose HJURP to be a cell-cycle-regulated CENP-A-specific histone chaperone required for centromeric chromatin assembly...
  37. pmc Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N
    Christopher W Carroll
    Department of Biochemistry, Stanford University School of Medicine, Beckman Center, Palo Alto, CA 94503 5307, USA
    Nat Cell Biol 11:896-902. 2009
    ..These data suggest that CENP-N interprets the information encoded within CENP-A nucleosomes and recruits other proteins to centromeric chromatin that are required for centromere function and propagation...
  38. pmc Double-strand DNA breaks recruit the centromeric histone CENP-A
    Samantha G Zeitlin
    Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 106:15762-7. 2009
    ..Finally, since cell survival after radiation-induced DNA damage correlates with CENP-A expression level, we propose that CENP-A may have a function in DNA repair...
  39. pmc CENPA overexpression promotes genome instability in pRb-depleted human cells
    Angela Amato
    Dipartimento di Biologia Cellulare e dello Sviluppo, Palermo, Italy
    Mol Cancer 8:119. 2009
    ..Functional inactivation of the Retinoblastoma protein (pRb) has been indicated as a cause promoting chromosomal instability as well centrosome amplification. However, the underlying molecular mechanism still remains to be clarified...
  40. doi Building centromeres: home sweet home or a nomadic existence?
    Alessia Buscaino
    Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Scotland, UK
    Curr Opin Genet Dev 20:118-26. 2010
    ..We focus on what influences the choice of site for CENP-A deposition and therefore the site of centromere formation. We then briefly discuss how centromeres are propagated once the site of centromere assembly is chosen...
  41. pmc Centromere protein A dynamics in human pluripotent stem cell self-renewal, differentiation and DNA damage
    Gayane Ambartsumyan
    Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA
    Hum Mol Genet 19:3970-82. 2010
    ....
  42. pmc Tetrameric organization of vertebrate centromeric nucleosomes
    Emilios K Dimitriadis
    Scanning Probe Microscopy Unit, Biomedical Engineering and Physical Science Shared Resource, National Institute for Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 107:20317-22. 2010
    ..In centromeres, canonical histone H3 is replaced in nucleosomes by a centromere-specific histone H3 variant (CENH3), providing the unique epigenetic signature required for microtubule binding...
  43. pmc Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP
    Hao Hu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Genes Dev 25:901-6. 2011
    ..These findings provide key information for specific recognition of CENP-A and mechanistic insights into the process of centromeric chromatin assembly...
  44. pmc HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore
    Meghan C Barnhart
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, VA 22908, USA
    J Cell Biol 194:229-43. 2011
    ....
  45. doi Formation of novel CENP-A domains on tandem repetitive DNA and across chromosome breakpoints on human chromosome 8q21 neocentromeres
    Dan Hasson
    Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, Icahn Medical Institute, NY 10029, USA
    Chromosoma 120:621-32. 2011
    ..This neocentromere suggests that chromosomal rearrangement and DNA breakage may be involved in neocentromere formation. These novel examples provide insight into the formation and structure of human neocentromeres...
  46. doi Clinical correlates of CENP-A and CENP-B antibodies in a large cohort of patients with systemic sclerosis
    Marie Hudson
    Jewish General Hospital, Room A 725, 3755 Cote Ste Catherine Road, Montreal, Quebec H3T 1E2, Canada
    J Rheumatol 39:787-94. 2012
    ..To study the clinical phenotypes of centromeric proteins (CENP)-A- and CENP-B-positive patients with systemic sclerosis (SSc) and to compare them to anticentromere antibody (ACA)-positive and negative SSc patients...
  47. doi Expression and prognostic significance of centromere protein A in human lung adenocarcinoma
    Qing Wu
    Department of Respiratory Medicine, Hangzhou Hospital of Traditional Chinese Medicine, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310007, PR China
    Lung Cancer 77:407-14. 2012
    ..The aim of the present study was to explore the expression and prognostic significance of CENP-A in lung adenocarcinoma...
  48. pmc Centromere protein-A, an essential centromere protein, is a prognostic marker for relapse in estrogen receptor-positive breast cancer
    Susan L McGovern
    Department of Radiation Oncology, 1515 Holcombe Blvd, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA
    Breast Cancer Res 14:R72. 2012
    ..This study was undertaken to determine if CENP-A is a prognostic factor for breast cancer patients not receiving systemic therapy or predictive of response to tamoxifen or neoadjuvant chemotherapy...
  49. pmc CENP-A confers a reduction in height on octameric nucleosomes
    Matthew D D Miell
    Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK
    Nat Struct Mol Biol 20:763-5. 2013
    ..We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes...
  50. doi Mislocalization of the centromeric histone variant CenH3/CENP-A in human cells depends on the chaperone DAXX
    Nicolas Lacoste
    Institut Curie, Centre de Recherche, Paris75248, France CNRS, UMR3664, Paris75248, France Equipe Labellisée Ligue Contre le Cancer, UMR3664, Paris 75248, France UPMC, UMR3664, Paris 75248, France
    Mol Cell 53:631-44. 2014
    ..Their definition relies on the presence of a centromere-specific H3 histone variant CenH3, known as CENP-A in mammals...
  51. ncbi Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0
    P Lomonte
    Medical Research Council Virology Unit, Glasgow G11 5JR, Scotland, United Kingdom
    J Biol Chem 276:5829-35. 2001
    ....
  52. ncbi Molecular behavior in living mitotic cells of human centromere heterochromatin protein HPLalpha ectopically expressed as a fusion to red fluorescent protein
    K Sugimoto
    Division ofApplied Biochemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan
    Cell Struct Funct 26:705-18. 2001
    ..These results suggested that, although the majority of HP1alpha diffuses into the cytoplasm, some populations are retained in the centromeric region and involved in the association and segregation of sister kinetochores during mitosis...
  53. doi Centromeres: assembling and propagating epigenetic function
    Macdara Glynn
    Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland
    Subcell Biochem 50:223-49. 2010
    ..nucleosome not found elsewhere in the genome, in which histone H3 is replaced with a homologue - CENP-A or CenH3. This domain is flanked by heterochromatin and is folded to provide a 3-dimensional cylinder-like structure at ..
  54. pmc Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro
    K Yoda
    BioScience Center, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Proc Natl Acad Sci U S A 97:7266-71. 2000
    ..Thus, we conclude that CENP-A forms an octameric complex with histones H4, H2A, and H2B in the presence of DNA...
  55. pmc A paucity of heterochromatin at functional human neocentromeres
    Alicia Alonso
    Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA
    Epigenetics Chromatin 3:6. 2010
    ..Large domains of heterochromatin also do not appear necessary for centromere function. Thus, this study provides important insight into the structural requirements of human centromere function...
  56. doi ZNF397, a new class of interphase to early prophase-specific, SCAN-zinc-finger, mammalian centromere protein
    S L Bailey
    Chromosome and Chromatin Research, Murdoch Childrens Research Institute, Department of Paediatrics, University of Melbourne, Royal Children s Hospital, Parkville, Victoria, Australia
    Chromosoma 117:367-80. 2008
    ..The possible role of this protein in transcription at the centromere is discussed...
  57. ncbi CENP-G: a new centromeric protein that is associated with the alpha-1 satellite DNA subfamily
    D He
    Department of Cell Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Chromosoma 107:189-97. 1998
    ..CENP-G was also detected in CHO, Indian muntjac and Chinese muntjac cells, suggesting that it is conserved in evolution...
  58. ncbi Characterization of chicken CENP-A and comparative sequence analysis of vertebrate centromere-specific histone H3-like proteins
    Vinciane Regnier
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, UK
    Gene 316:39-46. 2003
    ....
  59. ncbi The mammalian centromere: structural domains and the attenuation of chromatin modeling
    A A Van Hooser
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    FASEB J 13:S216-20. 1999
    ..This specialized centromere subdomain would be predicted to have a very tight and static nucleosome structure as a result of the absence of H3 phosphorylation and acetylation...
  60. pmc Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4
    Araceli G Castillo
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Genet 3:e121. 2007
    ..Thus, CENP-A(Cnp1) chromatin assembly in fission yeast exhibits plasticity with respect to the underlying sequences and is sensitive to the levels of CENP-A(Cnp1) and other core histones...
  61. pmc CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1
    Vinciane Regnier
    Department of Biochemistry, Oxford University, South Parks Road, OX1 3QU Oxford, United Kingdom
    Mol Cell Biol 25:3967-81. 2005
    ..Our data thus point to a specific role for CENP-A in assembly of kinetochores competent in the maintenance of mitotic checkpoint signaling...
  62. pmc Proteolysis restricts localization of CID, the centromere-specific histone H3 variant of Drosophila, to centromeres
    Olga Moreno-Moreno
    Institute of Molecular Biology of Barcelona IBMB, CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain
    Nucleic Acids Res 34:6247-55. 2006
    ..However, cells showing reduced amounts of mislocalized CID show normal cell cycle progression...
  63. pmc Dissection of CENP-C-directed centromere and kinetochore assembly
    Kirstin J Milks
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Biol Cell 20:4246-55. 2009
    ..We find that the amino terminus of CENP-C promotes kinetochore assembly by ensuring proper targeting of the Mis12/MIND complex and CENP-K...
  64. ncbi Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere--kinetochore complexes
    N Sugata
    Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, 3 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Hum Mol Genet 9:2919-26. 2000
    ..These results suggest that CENP-H multimers localize constitutively to the inner kinetochore plate and play an important fundamental role in organization and function of the active human centromere-kinetochore complex...
  65. ncbi Relevance of histone acetylation and replication timing for deposition of centromeric histone CENP-A
    Ilia I Ouspenski
    Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Exp Cell Res 285:175-88. 2003
    ..Thus histone acetylation and replication timing are not sufficient for specifying the sites of CENP-A deposition and likely for centromere identity...
  66. ncbi The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres
    Hiroshi Masumoto
    Laboratory of Biosystems and Cancer, National Cancer Institute, National Institutes of Health, Bldg 37, Rm 5040, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Chromosome Res 12:543-56. 2004
    ..Mechanisms to link the centromere tightly to specific genomic sequences exist in humans and the two yeast species...
  67. ncbi Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences
    Yoshinori Tanaka
    Protein Research Group, RIKEN Genomic Sciences Center, 1 7 22 Suehiro cho, Tsurumi, Yokohama 230 0045, Japan
    J Biol Chem 280:41609-18. 2005
    ..Therefore, CENP-B may be a determinant for translational positioning of the centromere-specific nucleosomes through its binding to the nucleosomal CENP-B box...
  68. ncbi Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells
    Hiroshi Izuta
    Bioscience and Biotechnology Center, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    Genes Cells 11:673-84. 2006
    ..These results suggest that the seven components of the ICEN complex are predominantly localized at the centromeres and are required for kinetochore function perhaps through or not through loading of CENP-H and hMis6 onto the centromere...
  69. pmc Active establishment of centromeric CENP-A chromatin by RSF complex
    Marinela Perpelescu
    Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi, Japan
    J Cell Biol 185:397-407. 2009
    ..From these data, we propose the RSF complex as a new factor actively supporting the assembly of CENP-A chromatin...
  70. pmc Histone modifications within the human X centromere region
    Brankica Mravinac
    Institute for Genome Sciences and Policy, Duke University, Durham, NC, USA
    PLoS ONE 4:e6602. 2009
    ..Our results indicate that the human X centromere contains multiple types of chromatin, is organized similarly to smaller eukaryotic centromeres, and responds to structural changes by expanding or contracting domains...
  71. doi Haploid plants produced by centromere-mediated genome elimination
    Maruthachalam Ravi
    Department of Plant Biology, University of California, Davis, Davis, California 95616, USA
    Nature 464:615-8. 2010
    ..can be easily generated through seeds by manipulating a single centromere protein, the centromere-specific histone CENH3 (called CENP-A in human)...
  72. ncbi Assay of centromere function using a human artificial chromosome
    H Masumoto
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    Chromosoma 107:406-16. 1998
    ..We conclude that alpha21-I alphoid DNA can induce de novo assembly of active centromere/kinetochore structures on minichromosomes...
  73. ncbi Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells
    Tetsuya Hori
    PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    J Cell Sci 116:3347-62. 2003
    ..Furthermore, photobleaching experiments revealed that the Nuf2-Hec1 complex is stably associated with the centromere and that interaction of this complex with the centrosome is dynamic...
  74. ncbi Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein
    K Sugimoto
    Division of Applied Biochemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan
    Cell Struct Funct 25:253-61. 2000
    ..These cell lines will further be used for visualizing the prekinetochore locus in interphase nuclei as well as analyzing kinetochore dynamics in the living cells...
  75. ncbi [Expression of centromere protein A in hepatocellular carcinoma]
    Yong Mei Li
    Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
    Zhonghua Bing Li Xue Za Zhi 36:175-8. 2007
    ..To study the expression of centromere protein A (CENP-A) and its significance in hepatocellular carcinoma (HCC) and adjacent non-neoplastic liver tissue...
  76. pmc Centromere identity in Drosophila is not determined in vivo by replication timing
    B Sullivan
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    J Cell Biol 154:683-90. 2001
    ....
  77. ncbi Centromeres and variant histones: what, where, when and why?
    M Mitchell Smith
    Department of Microbiology and University of Virginia Cancer Center, Jordan Building, Room 7223, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA
    Curr Opin Cell Biol 14:279-85. 2002
    ..Finally, a new report provides a glimpse into the potential regulation of CENP-A through specific post-translational phosphorylation, suggesting a broad level of control through histone tail modifications...
  78. pmc Two distinct pathways responsible for the loading of CENP-A to centromeres in the fission yeast cell cycle
    Kohta Takahashi
    Division of Cell Biology, Institute of Life Science, Kurume University, 1 1 Hyakunen kohen, Kurume, Fukuoka 839 0864, Japan
    Philos Trans R Soc Lond B Biol Sci 360:595-606; discussion 606-7. 2005
    ..Here, we discuss the functional relationship between the flexible loading mechanism of CENP-A and the plasticity of centromere chromatin formation in fission yeast...
  79. ncbi Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase
    Melina Schuh
    Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Curr Biol 17:237-43. 2007
    ..Thus, our findings uncover a strikingly dynamic behavior of centromere components in anaphase...
  80. doi The histone variant CENP-A and centromere specification
    Ben E Black
    Department of Biochemistry and Biophysics and Program in Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Opin Cell Biol 20:91-100. 2008
    ..Centromere identity, itself, is propagated during mitotic exit and early G1, and it relies upon a cis-acting targeting domain within CENP-A and a proposed centromere 'priming' reaction...
  81. pmc Three-dimensional localization of CENP-A suggests a complex higher order structure of centromeric chromatin
    Owen J Marshall
    Chromosome and Chromatin Research, Murdoch Childrens Research Institute, Royal Children s Hospital, Parkville, Victoria 3052, Australia
    J Cell Biol 183:1193-202. 2008
    ..C., L.H. Wong, N. Wong, and K.H.A. Choo. 2005. Hum. Mol. Genet. 14:85-93), our data suggest that centromeric chromatin is arranged in a coiled 30-nm fiber that is itself coiled or folded to form a higher order structure...
  82. doi CENP-O, a protein localized at the centromere throughout the cell cycle, is a novel target antigen in systemic sclerosis
    Akiko Saito
    Department of Dermatology, Nagoya University Graduate School of Medicine, 65 Turumai cho Showa ku, Nagoya, 466 8550, Japan
    J Rheumatol 36:781-6. 2009
    ..The CENP-O protein is a component of the ICEN that localizes at the centromere throughout the cell cycle. We investigated whether CENP-O is also targeted by sera from patients with systemic autoimmune diseases...
  83. doi Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1
    Ralf B Schittenhelm
    Institute of Molecular Life Sciences, University of Zurich, CH 8057 Zurich, Switzerland
    J Cell Sci 123:3768-79. 2010
    ..eukaryotes depends critically on the faithful incorporation of a centromere-specific histone H3 variant encoded by CENPA in humans and cid in Drosophila...
  84. doi A small GTPase molecular switch regulates epigenetic centromere maintenance by stabilizing newly incorporated CENP-A
    Anaïck Lagana
    Institute for Research in Immunology and Cancer, Université de Montréal P O Box 6128, Station Centre Ville Montréal QC, H3C 3J7 Canada
    Nat Cell Biol 12:1186-93. 2010
    ..Thus, a small GTPase switch ensures epigenetic centromere maintenance after loading of new CENP-A...
  85. pmc CENP-B: a major human centromere protein located beneath the kinetochore
    C A Cooke
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Cell Biol 110:1475-88. 1990
    ..This difference in staining pattern may reflect the presence of a "collar" of chromatin rich in CENP-C and/or CENP-A encircling the kinetochore plates...
  86. ncbi Conservation of centromere protein in vertebrates
    R Saffery
    The Murdoch Institute, Royal Children s Hospital, Parkville, Australia
    Chromosome Res 7:261-5. 1999
    ....
  87. pmc CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells
    T Fukagawa
    National Institute of Genetics and Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    EMBO J 20:4603-17. 2001
    ..These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H...
  88. pmc Adaptive evolution of centromere proteins in plants and animals
    Paul B Talbert
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    J Biol 3:18. 2004
    ..The nucleosomes of centromeres are characterized by a special H3-like histone (CenH3), which evolves rapidly and adaptively in Drosophila and Arabidopsis...
  89. pmc Identification of xenopus CENP-A and an associated centromeric DNA repeat
    Nathaniel S Edwards
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Biol Cell 16:1800-10. 2005
    ..Fluorescent in situ hybridization with Fcr1 probes stained most centromeres in cultured cells. By staining lampbrush chromosomes, we specifically identified the 11 (of 18) chromosomes that stain consistently with Fcr1 probes...
  90. pmc The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis
    Yoshikazu Mikami
    PRESTO of JST, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:1958-70. 2005
    ..On the basis of these results and previously published data, we propose that the Nuf2 complex functions as a connector between the inner and outer kinetochores...
  91. pmc The constitutive centromere component CENP-50 is required for recovery from spindle damage
    Yukinori Minoshima
    National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Mol Cell Biol 25:10315-28. 2005
    ..We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage...
  92. ncbi Centromere size and position in Candida albicans are evolutionarily conserved independent of DNA sequence heterogeneity
    Prashant K Mishra
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA
    Mol Genet Genomics 278:455-65. 2007
    ..The chromosomal locations and the sizes of CEN DNAs have remained conserved, in agreement with the idea that CEN function in C. albicans is templated by heritable epigenetic mechanisms...
  93. pmc A high-resolution map of nucleosome positioning on a fission yeast centromere
    Jun S Song
    Department of Biostatistics and Computational Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Genome Res 18:1064-72. 2008
    A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S...
  94. pmc Adaptive evolution of foundation kinetochore proteins in primates
    Mary G Schueler
    Genome Technology Branch, National Institutes of Health, Bethesda, MD, USA
    Mol Biol Evol 27:1585-97. 2010
    ..These findings point to important and distinct evolutionary forces operating on the DNA and proteins of the primate centromere...
  95. ncbi Beyond the ABCs of CKC and SCC. Do centromeres orchestrate sister chromatid cohesion or vice versa?
    Pamela B Meluh
    Memorial Sloan Kettering Cancer Center, Laboratory of Mechanism and Regulation of Mitosis, New York, NY 10021, USA
    Eur J Biochem 269:2300-14. 2002
    ..Here we review the nature of centromeric chromatin, the dynamics and regulation of sister chromatid cohesion, and the relationship between the two...
  96. pmc Dynamics of inner kinetochore assembly and maintenance in living cells
    Peter Hemmerich
    Leibniz Institute for Age Research, Fritz Lipmann Institute, 07745 Jena, Germany i leibniz de
    J Cell Biol 180:1101-14. 2008
    ..Our data reveal a wide range of cell cycle-specific assembly plasticity of the centromere that provides both stability through sustained binding of some components and flexibility through dynamic exchange of other components...
  97. doi Epigenetics regulate centromere formation and kinetochore function
    Randall S Gieni
    Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, Canada
    J Cell Biochem 104:2027-39. 2008
    ..substitution of histone H3 with the centromere specific H3 variant centromere protein A (CENP-A), also known as CENH3. Kinetochore formation and function are dependent on a variety of regional epigenetic modifications that appear to ..
  98. pmc Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation
    Sylvia Erhardt
    Department of Genome Dynamics, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    J Cell Biol 183:805-18. 2008
    ..Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division...
  99. ncbi Histone variants--the structure behind the function
    Juan Ausio
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
    Brief Funct Genomic Proteomic 5:228-43. 2006
    ..Understanding and deciphering the underlying structural amino acid code behind such variability remains one of the most exciting future challenges in chromatin research...
  100. pmc H2A.Z contributes to the unique 3D structure of the centromere
    Ian K Greaves
    The John Curtin School of Medical Research, Australian National University, P O Box 334, Canberra, The Australian Capital Territory 2601, Australia
    Proc Natl Acad Sci U S A 104:525-30. 2007
    ..A second region of H2A.Z is found, with trimethyl K9 H3 at the inner centromere. We therefore propose that H2A.Z plays an integral role in organizing centromere structure...
  101. doi Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis
    Qiang Chen
    The MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio membrane and Membrane Bio engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 18:268-80. 2008
    ..Cyclin B1 is then transported from each kinetochore as microtubule attachment is completed, and this relocalization may redirect the activity of cyclin B1-CDK1 and contribute to inactivation of the spindle assembly checkpoint...

Research Grants36

  1. Molecular Architecture of the Regional Kinetochore in Fission Yeast
    Xiangwei He; Fiscal Year: 2012
    ..This project will be conducted in a single cell organism, the fission yeast S.pombe, which is an excellent model for the study of the regional kinetochores, the type found in humans. ..
  2. Aaron F Straight; Fiscal Year: 2016
    ..Our proposal is focused on understanding the molecular mechanisms that ensure accurate chromosome segregation to inform how chromosome missegregation arises and contributes to human disease. ..
  3. Molecular Mechanisms of Chromosome Segregation in Yeast
    Jennifer L Gerton; Fiscal Year: 2012
    ..Cancer Res, 2005. 65: p. 4683-9. 3. Heun, P., et al., Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores. Dev Cell, 2006. 10: p. 303-15. ..
  4. SUSAN BIGGINS; Fiscal Year: 2015
    ..This work will not only elucidate important aspects about the process of segregation, but will aid in the design of better therapeutic interventions in the long-term. ..
  5. Don W Cleveland; Fiscal Year: 2016
    ....
  6. Non-coding RNAs in the epigenetics of human centromere formation
    PETER WARBURTON; Fiscal Year: 2009
    ..Surrounding the CENPA domain is the centromeric heterochromatin, characterized by histone H3 lysine 9 methylation (H3K9me) and ..
  7. Ben E Black; Fiscal Year: 2016
    ....
  8. Donald F Hunt; Fiscal Year: 2016
    ..Accordingly, we will conduct experiments to identify these proteins and determine the n-terminal motif required for trimethylation by NRMT. ..
  9. AUTOIMMUNE ANTIGENS AND SYSTEMIC SCLEROSIS
    SALLIE HOCH; Fiscal Year: 1993
    ..Overall, these studies are asking what features make the centromere antigens targets in the autoimmune sequence of events, while relating these same polypeptides to the native cellular environment in which they function...
  10. FUNCTIONAL ANALYSIS OF CENTROMERIC CHROMATIN IN YEAST
    Molly Fitzgerald Hayes; Fiscal Year: 2003
    ..Scm3 is structurally related to Nap1, a chromatin assembly factor that associates with histones and facilitates formation of the nucleosome. A model is proposed for targeting of Cse4 by Scm3 that will be tested in aim 3. ..
  11. The role of CENP-A in mammalian centromere specification
    DANIEL FOLTZ; Fiscal Year: 2005
    ..Specific Aim3: Finally, the studies proposed here are designed to determine whether CENP-A itself is sufficient to recruit centromere components and ultimately specify the centromere. ..
  12. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
    ..Chromosome motion will be monitored simultaneously using a GFPMicrotubules GFP-tagged kinetochore protein, CenpA. The movement of the photoactivated marks at prophase will be characterized and their role in nuclear envelope ..
  13. SISTER CHROMATID LINKAGE AND SEPARATION
    Andrew Murray; Fiscal Year: 2004
    ..This information will be directly relevant to understanding how aneuploidy is generated in Down syndrome and tumor progression. ..
  14. MOLECULAR ANALYSIS OF MAMMALIAN KINETOCHORE STRUCTURE
    Kevin Sullivan; Fiscal Year: 1992
    ..These experiments will establish tools and methods for detailed molecular analysis of the mechanisms of human chromosome segregation...
  15. Biochemical Basis of CENP-A Epigenetic Inheritance
    SAMANTHA ZEITLIN; Fiscal Year: 2005
    ..These studies will also have direct implications for understanding cancer causation, and applications in gene therapy. ..
  16. HISTONE GENE EXPRESSION IN YEAST
    M Smith; Fiscal Year: 2007
    ..To understand the rules and mechanisms for these assembly patterns, we will engineer mutations in specific cis- and trans-acting factors to test alternative models of Htz1 recruitment. ..
  17. A1 REGULATED LEUKOCYTE APOPTOSIS DURING INFLAMMATION
    Michael Prystowsky; Fiscal Year: 2001
    ..3) Protection of macrophages during inflammation is mediated by A1 and is vital for host defense. ..
  18. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  19. CENTROMERE STRUCTURE, ASSEMBLY AND FUNCTION IN YEAST
    Pamela Meluh; Fiscal Year: 2004
    ..5. To use Cse4p as a cytological marker for yeast centromere position. Cse4p localization will be characterized using indirect immunofluorescence, GFP fluorescence, and immuno-EM in wildtype and mutant cells. ..
  20. Assembly and Function of Human Centromere Chromatin
    Kevin Sullivan; Fiscal Year: 2004
    ....
  21. A 13q32 BAC Microarray for chromosome function analysis
    PETER WARBURTON; Fiscal Year: 2004
    ..The development of these types of high-density genomic microarrays will be a major step towards understanding the genomic organization and role of non-protein coding functional DNA elements in our cells. ..
  22. Structure and Function of a Eukaryotic Centromere
    Kerry S Bloom; Fiscal Year: 2010
    ....
  23. ANALYSIS AND MANIPULATION OF HUMAN NEOCENTROMERES
    K H Choo; Fiscal Year: 2006
    ..abstract_text> ..
  24. Organization and Regulation of Eukaryotic Centromeres
    BETH SULLIVAN; Fiscal Year: 2009
    ..We propose to investigate the roles of genomic structure and chromatin organization and dynamics in assembly and regulation of CENP-A chromatin, centromeric domains, and metaphase kinetochore structure. ..
  25. The Role of MYST histone acetyltransferases in genome stability
    M Smith; Fiscal Year: 2008
    ..These experiments will greatly expand our understanding of Hbo1 function in regulating DNA replication and cell proliferation. ..
  26. The repetitive DNA structure of the human genome
    PETER WARBURTON; Fiscal Year: 2009
    ..The studies proposed in this application will facilitate both computational and biological approaches to genomics and provide a unique analysis of a large and relatively neglected portion of our DNA sequence. ..
  27. Mechanism of Mitotic Checkpoint in Mammalian Cells
    TIMOTHY YEN; Fiscal Year: 2007
    ..2 Description, ..
  28. E coli-based vectors for BAC delivery to mammalian cells
    PETER WARBURTON; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  29. Epigenetic Regulation of Gene Expression During Early Mouse Embryogenesis
    M Smith; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  30. ANALYSIS OF HUMAN CHROMOSOME 13Q NEOCENTROMERE FORMATION
    PETER WARBURTON; Fiscal Year: 2007
    ..Investigation of human centromere structure, function and formation will greatly facilitate the construction of artificial human chromosomes for use as autonomous gene expression vectors. ..
  31. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  32. Proteomic analysis of head & neck squamous cell cancer
    Michael Prystowsky; Fiscal Year: 2005
    ....
  33. KINETOCHORE STRUCTURE AND FUNCTION
    TIMOTHY YEN; Fiscal Year: 2010
    ..We want to extend our studies to continue to address the major issues about how checkpoint proteins monitor kinetochore attachments, how the "wait anaphase" signal is generated and how errors in attachment are corrected. ..
  34. Assembling a Centric Nucleosome
    Richard Baker; Fiscal Year: 2004
    ..Given the high conservation of centric histone H3 variants throughout evolution, the results will illuminate the problem of centromere formation and function in higher eukaryotes including humans. ..
  35. E. COLI BASED VECTORS FOR GENE DELIVERY TO HUMAN CELLS
    PETER WARBURTON; Fiscal Year: 2002
    ..These studies propose to develop E. coli-based HAC vectors for human gene therapy that encompass novel approaches for gene delivery, accurate gene expression, and mitotic stability. ..