Cdc2

Summary

Gene Symbol: Cdc2
Description: cyclin dependent kinase 1
Alias: CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1, cell cycle controller CDC2, cell division control protein 2 homolog, cell division cycle 2, G1 to S and G2 to M, cell division protein kinase 1, p34 protein kinase
Species: human

Top Publications

  1. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
  2. pmc Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation
    M Xu
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    Mol Cell Biol 14:8420-31. 1994
  3. pmc HIV-1 induced bystander apoptosis
    Himanshu Garg
    Center of Excellence for Infectious Disease, Department of Biomedical Science, Texas Tech University Health Sciences Center, 5001 El Paso Dr, MSB 1 Annex, El Paso, TX 79905, USA
    Viruses 4:3020-43. 2012
  4. pmc Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit
    Ting Yu Yeh
    Department of Biology, Johns Hopkins University, Baltimore, MD, USA
    EMBO J 32:1023-35. 2013
  5. pmc Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition
    Ken Chih Chien Cheng
    Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, 725 N Wolfe Street, PCTB 706, Baltimore, MD 21205, USA
    Cell 139:560-72. 2009
  6. ncbi Phosphorylation of E2F-1 modulates its interaction with the retinoblastoma gene product and the adenoviral E4 19 kDa protein
    R Fagan
    Laboratory of Gene Regulation, Imperial Cancer Research Fund, London, England
    Cell 78:799-811. 1994
  7. pmc Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases
    Abdullah Yalcin
    Division of Medical Oncology Molecular Targets Group, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    J Biol Chem 284:24223-32. 2009
  8. pmc Inhibitory phosphorylation of cyclin-dependent kinase 1 as a compensatory mechanism for mitosis exit
    Jeremy P H Chow
    Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
    Mol Cell Biol 31:1478-91. 2011
  9. pmc CDK1 stabilizes HIF-1α via direct phosphorylation of Ser668 to promote tumor growth
    Noel A Warfel
    Department of Medicine Hematology Oncology Penn State Hershey Cancer Institute Penn State College of Medicine Hershey, PA USA
    Cell Cycle 12:3689-701. 2013
  10. pmc The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions
    Ayumi Kudoh
    Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Kanagawa, Japan
    Retrovirology 11:9. 2014

Research Grants

  1. Yun Fai Chris Lau; Fiscal Year: 2014
  2. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
  3. Characterization of JNK in Cell Cycle Control
    ZE apos EV A RONAI; Fiscal Year: 2012
  4. DAVID OWEN MORGAN; Fiscal Year: 2015
  5. The CAK Network in Metazoan Cell Cycle Regulation
    Robert P Fisher; Fiscal Year: 2010
  6. Regulation of M phase exit
    SALLY A KORNBLUTH; Fiscal Year: 2010
  7. Induction and maintenance of mitosis by Cdk1 suppression of Protein phosphatase 1
    ROBERT LEWIS MARGOLIS; Fiscal Year: 2012
  8. Regulation Of A Novel Xenopus Polo-Like Kinase 1 Substrate
    Junjun Liu; Fiscal Year: 2012
  9. Control of Mitotic Entry by Regulators of Cdc2
    SALLY A KORNBLUTH; Fiscal Year: 2010
  10. Regulation and function of the Clp 1p protein phosphatase
    Dannel McCollum; Fiscal Year: 2010

Detail Information

Publications304 found, 100 shown here

  1. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
    ....
  2. pmc Cyclin A/CDK2 binds directly to E2F-1 and inhibits the DNA-binding activity of E2F-1/DP-1 by phosphorylation
    M Xu
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    Mol Cell Biol 14:8420-31. 1994
    ..Finally, we report that the DNA-binding activity of the E2F-1/DP-1 complex is inhibited following phosphorylation by cyclin A/CDK2...
  3. pmc HIV-1 induced bystander apoptosis
    Himanshu Garg
    Center of Excellence for Infectious Disease, Department of Biomedical Science, Texas Tech University Health Sciences Center, 5001 El Paso Dr, MSB 1 Annex, El Paso, TX 79905, USA
    Viruses 4:3020-43. 2012
    ....
  4. pmc Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit
    Ting Yu Yeh
    Department of Biology, Johns Hopkins University, Baltimore, MD, USA
    EMBO J 32:1023-35. 2013
    ..We conclude that dynactin plays a previously undescribed regulatory role in the spindle assembly checkpoint by recruiting Plk1 to kinetochores and facilitating phosphorylation of important downstream targets...
  5. pmc Regulation of MBK-2/DYRK by CDK-1 and the pseudophosphatases EGG-4 and EGG-5 during the oocyte-to-embryo transition
    Ken Chih Chien Cheng
    Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, Center for Cell Dynamics, Johns Hopkins School of Medicine, 725 N Wolfe Street, PCTB 706, Baltimore, MD 21205, USA
    Cell 139:560-72. 2009
    ..Our findings link cell-cycle progression to MBK-2/DYRK activation and the oocyte-to-embryo transition...
  6. ncbi Phosphorylation of E2F-1 modulates its interaction with the retinoblastoma gene product and the adenoviral E4 19 kDa protein
    R Fagan
    Laboratory of Gene Regulation, Imperial Cancer Research Fund, London, England
    Cell 78:799-811. 1994
    ..These residues were phosphorylated in vivo and by p34cdc2 kinase in vitro...
  7. pmc Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases
    Abdullah Yalcin
    Division of Medical Oncology Molecular Targets Group, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    J Biol Chem 284:24223-32. 2009
    ..Taken together, these observations demonstrate an unexpected role for PFKFB3 in nuclear signaling and indicate that Fru-2,6-BP may couple the activation of glucose metabolism with cell proliferation...
  8. pmc Inhibitory phosphorylation of cyclin-dependent kinase 1 as a compensatory mechanism for mitosis exit
    Jeremy P H Chow
    Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
    Mol Cell Biol 31:1478-91. 2011
    ..These findings indicate that, in the absence of sufficient APC/C activity, an alternative mechanism that utilized the classic inhibitory phosphorylation of CDK1 could mediate mitotic exit...
  9. pmc CDK1 stabilizes HIF-1α via direct phosphorylation of Ser668 to promote tumor growth
    Noel A Warfel
    Department of Medicine Hematology Oncology Penn State Hershey Cancer Institute Penn State College of Medicine Hershey, PA USA
    Cell Cycle 12:3689-701. 2013
    ....
  10. pmc The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions
    Ayumi Kudoh
    Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Kanagawa, Japan
    Retrovirology 11:9. 2014
    ..In our present study, we identified this kinase using a proteomic approach and further delineate its role in HIV-1 replication...
  11. ncbi Regulation of mitotic function of Chk1 through phosphorylation at novel sites by cyclin-dependent kinase 1 (Cdk1)
    Takashi Shiromizu
    Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464 8681, Japan
    Genes Cells 11:477-85. 2006
    ..These observations imply the regulation of mitotic Chk1 function through Chk1 phosphorylation at novel sites by Cdk1...
  12. ncbi Phosphorylation of HIV-1 Rev protein: implication of protein kinase CK2 and pro-directed kinases
    F Meggio
    Dipartimento di Chimica Biologica, Universita di Padova, Italy
    Biochem Biophys Res Commun 226:547-54. 1996
    ..Protein kinase CK1 cannot replace CK2 as phosphorylating agent and cdc2 only slowly phosphorylates Rev at one of the two sites affected by MAP kinase...
  13. pmc Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome
    Yakir Moshe
    Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 101:7937-42. 2004
    ..Transfection with an small interfering RNA duplex directed against Plk1 caused the accumulation of Emi1 in mitotically arrested HeLa cells. It is suggested that phosphorylation of Emi1 by Plk1 is involved in its degradation in mitosis...
  14. pmc New Cdc2 Tyr 4 phosphorylation by dsRNA-activated protein kinase triggers Cdc2 polyubiquitination and G2 arrest under genotoxic stresses
    Cheol Hee Yoon
    Department of Biological Sciences, Sungkyunkwan University, Suwon, Gyeonggi Do, South Korea
    EMBO Rep 11:393-9. 2010
    Cell division cycle 2 (Cdc2) protein is an essential subunit of M-phase kinase (MPK), which has a key role in G2/M transition...
  15. pmc HIV-1 accessory protein Vpr: relevance in the pathogenesis of HIV and potential for therapeutic intervention
    Michael Kogan
    Department of Neuroscience, Department of Neuroscience, Center for Neurovirology, Temple University School of Medicine, 3500 North Broad Street, Philadelphia, PA 19140, USA
    Retrovirology 8:25. 2011
    ..In view of the pivotal functions of Vpr in virus infection, replication, and persistence of infection, this protein represents an attractive target for therapeutic intervention...
  16. pmc Interaction of Sororin protein with polo-like kinase 1 mediates resolution of chromosomal arm cohesion
    Nenggang Zhang
    Texas Children s Cancer and Hematology Center, Department of Pediatric Hematology Oncology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 286:41826-37. 2011
    ....
  17. pmc Phosphorylation of the myristoylated protein kinase C substrate MARCKS by the cyclin E-cyclin-dependent kinase 2 complex in vitro
    S Manenti
    INSERM CJF 95 10, IFR 30, Hopital Purpan, Pavillon Rayer, 31059 Toulouse, France
    Biochem J 340:775-82. 1999
    ..In contrast, phosphorylation of MARCKS by Cdk2 did not significantly affect further phosphorylation by PKC...
  18. ncbi Abolishment of the Tyr-15 inhibitory phosphorylation site on cdc2 reduces the radiation-induced G(2) delay, revealing a potential checkpoint in early mitosis
    Lynda Fletcher
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 62:241-50. 2002
    b>cdc2 is inactivated before mitosis by phosphorylation at its inhibitory sites, Thr-14 and Tyr-15. Irradiation prevents HeLa cells from completing the G(2)-M transition, and they arrest in G(2)...
  19. pmc Flavopiridol synergizes with sorafenib to induce cytotoxicity and potentiate antitumorigenic activity in EGFR/HER-2 and mutant RAS/RAF breast cancer model systems
    Teddy S Nagaria
    Division of Cancer Biology and Genetics, Queen s Cancer Research Institute, Queen s University, Kingston, Ontario, Canada
    Neoplasia 15:939-51. 2013
    ..These findings support the development of CDK and Raf co-targeting strategies in EGFR/HER-2-overexpressing or RAS/RAF mutant BCs...
  20. ncbi Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement
    G Draetta
    Cold Spring Harbor Laboratory, New York 11724
    Cell 54:17-26. 1988
    HeLa cell p34, homolog of the yeast cdc2+/CDC28 protein kinase, has been investigated. p34 was phosphorylated at two or more sites and existed in a complex with p13, the previously identified homolog of the suc1+ gene product of S. pombe...
  21. ncbi DNA damage-induced down-regulation of human Cdc25C and Cdc2 is mediated by cooperation between p53 and maintenance DNA (cytosine-5) methyltransferase 1
    Gerald Le Gac
    New England Biolabs, Ipswich, Massachusetts 01938, USA
    J Biol Chem 281:24161-70. 2006
    The Cdc25C phosphatase mediates cellular entry into mitosis in mammalian cells. Cdc25C activates Cdc2 for entry into mitosis by dephosphorylating Thr and Tyr at the site of inhibitory phosphorylation...
  22. pmc The decision to enter mitosis: feedback and redundancy in the mitotic entry network
    Arne Lindqvist
    Department of Medical Oncology, University Medical Center Utrecht, 3584 CG Utrecht, Netherlands
    J Cell Biol 185:193-202. 2009
    ....
  23. pmc Cyclin-dependent kinase-1 (Cdk1)/cyclin B1 dictates cell fate after mitotic arrest via phosphoregulation of antiapoptotic Bcl-2 proteins
    Nandini Sakurikar
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Biol Chem 287:39193-204. 2012
    ....
  24. pmc Ubp-M serine 552 phosphorylation by cyclin-dependent kinase 1 regulates cell cycle progression
    Yang Xu
    Department of Biochemistry and Molecular Genetics University of Alabama at Birmingham Birmingham, AL USA State Key Laboratory of Cellular and Molecular Biology College of Life Science Hebei Normal University Shijiazhuang, China
    Cell Cycle 12:3219-27. 2013
    ..Therefore, these studies confirm that Ubp-M is phosphorylated at S552 and identify CDK1 as the enzyme responsible for the phosphorylation. Importantly, this study specifically links Ubp-M S552P to cell cycle G 2/M phase progression...
  25. pmc Targeting cyclin-dependent kinase 1 (CDK1) but not CDK4/6 or CDK2 is selectively lethal to MYC-dependent human breast cancer cells
    Jian Kang
    The Kinghorn Cancer Centre and Cancer Research Division, Garvan Institute of Medical Research, 370 Victoria Street, Darlinghurst, Sydney, NSW, Australia
    BMC Cancer 14:32. 2014
    ..Recent studies have identified several cell cycle kinases as MYC synthetic-lethal genes. We therefore investigated the therapeutic potential of specific cyclin-dependent kinase (CDK) inhibition in MYC-driven breast cancer...
  26. ncbi Human immunodeficiency virus type-1 accessory protein Vpr: a causative agent of the AIDS-related insulin resistance/lipodystrophy syndrome?
    Tomoshige Kino
    Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 1583, USA
    Ann N Y Acad Sci 1024:153-67. 2004
    ....
  27. ncbi Cdc2/cyclin B1 interacts with and modulates inositol 1,4,5-trisphosphate receptor (type 1) functions
    Krishnamurthy Malathi
    Vascular Biology Laboratory, Department of Neurosurgery, St Luke s Roosevelt Hospital Center, New York, NY 10025, USA
    J Immunol 175:6205-10. 2005
    ..We have shown previously that the cyclin-dependent kinase 1/cyclin B (cdk1/CyB or cdc2/CyB) complex phosphorylates IP3R1 in vitro and in vivo at Ser421 and Thr799...
  28. pmc CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage
    Bing Yu
    Department of Medicine, Stony Brook University School of Medicine, Stony Brook, New York 11794 8160, USA
    Cancer Res 72:5448-53. 2012
    ..Our findings offer key new insights into how DDR is controlled during mitosis...
  29. pmc Human immunodeficiency virus 1 envelope-initiated G2-phase programmed cell death
    V Kolesnitchenko
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 92:11889-93. 1995
    ..Cells rapidly accumulate cyclin B protein and tyrosine-hyperphosphorylated p34cdc2 (cdk1) kinase, indicative of cell cycle arrest at G2 phase...
  30. ncbi Partner molecules of accessory protein Vpr of the human immunodeficiency virus type 1
    Tomoshige Kino
    Human Retrovirus Section, Center for Basic Research, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    DNA Cell Biol 23:193-205. 2004
    ..Further studies on Vpr function are needed to fully assess the function of this important early regulatory molecule of HIV and other lentiviruses...
  31. pmc Cyclin B-dependent kinase 1 regulates human TRF1 to modulate the resolution of sister telomeres
    Megan McKerlie
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    Nat Commun 2:371. 2011
    ..Our work suggests that Cdk1 controls TRF1 association with telomeres to facilitate temporal telomere de-protection, which is essential for sister telomere resolution...
  32. pmc Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1
    Mourad Sanhaji
    Department of Gynecology and Obstetrics, School of Medicine, J W Goethe University, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Mol Cell Biol 30:2594-607. 2010
    ..This is the first study demonstrating that Cdk1 regulates the localization and activity of MCAK in mitosis by directly phosphorylating the catalytic core domain of MCAK...
  33. pmc The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex
    F Liu
    Department of Cell Biology and Physiology, Washington University Medical School, St Louis, Missouri 63110 1093, USA
    Mol Cell Biol 17:571-83. 1997
    Entry into mitosis requires the activity of the Cdc2 kinase. Cdc2 associates with the B-type cyclins, and the Cdc2-cyclin B heterodimer is in turn regulated by phosphorylation...
  34. ncbi Phosphorylation of Cdc20/fizzy negatively regulates the mammalian cyclosome/APC in the mitotic checkpoint
    Y Yudkovsky
    Unit of Biochemistry, B Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa, 31096, Israel
    Biochem Biophys Res Commun 271:299-304. 2000
    ..It is suggested that inhibitory phosphorylation of Cdc20/Fizzy may have a role in keeping the cyclosome inactive in early mitosis and under conditions of mitotic checkpoint arrest...
  35. pmc Reciprocal activation by cyclin-dependent kinases 2 and 7 is directed by substrate specificity determinants outside the T loop
    S Garrett
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Mol Cell Biol 21:88-99. 2001
    ..kinase 7 (CDK7) is the catalytic subunit of the metazoan CDK-activating kinase (CAK), which activates CDKs, such as CDC2 and CDK2, through phosphorylation of a conserved threonine residue in the T loop...
  36. ncbi BRCA1-mediated G2/M cell cycle arrest requires ERK1/2 kinase activation
    Ying Yan
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Oncogene 24:3285-96. 2005
    ..These results implicate a critical role of ERK1/2 signaling in the regulation of BRCA1 function on controlling the G2/M checkpoint responses...
  37. ncbi Inhibition of Hsp90 function by ansamycins causes downregulation of cdc2 and cdc25c and G(2)/M arrest in glioblastoma cell lines
    P Garcia-Morales
    Instituto de Biologia Molecular y Celular, Universidad Miguel Hernandez, Elche, Alicante, Spain
    Oncogene 26:7185-93. 2007
    ..The protein levels of cell division cycle 2 (cdc2) kinase and cell division cycle 25c (cdc25c) were downregulated upon GA and 17-AAG treatment and cdc2 kinase ..
  38. ncbi Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis
    Y H Ling
    Department of Thoracic Head and Neck Medical Oncology, Section of Experimental Therapy, The University of Texas M D Anderson Cancer Center, Houston, Texas, 77030, USA
    J Biol Chem 273:18984-91. 1998
    ..The time curves of paclitaxel-induced cyclin B1 accumulation and stimulation of Cdc2/cyclin B1 kinase activity were identical and superimposable to that of M phase arrest...
  39. pmc Mitotic regulation of the APC activator proteins CDC20 and CDH1
    E R Kramer
    Research Institute of Molecular Pathology, A 1030 Vienna, Austria
    Mol Biol Cell 11:1555-69. 2000
    ..These mechanisms can explain the temporal order of APC activation by CDC20 and CDH1 and may help to ensure that exit from mitosis is not initiated before anaphase has occurred...
  40. pmc p53 regulation of G(2) checkpoint is retinoblastoma protein dependent
    P M Flatt
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Mol Cell Biol 20:4210-23. 2000
    ..The mechanism was p21 and retinoblastoma protein (pRB) dependent and involved an initial inhibition of cyclin B1-Cdc2 activity and a secondary decrease in cyclin B1 and Cdc2 levels...
  41. ncbi Specific phosphorylation of nucleophosmin on Thr(199) by cyclin-dependent kinase 2-cyclin E and its role in centrosome duplication
    Y Tokuyama
    Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267 0521, USA
    J Biol Chem 276:21529-37. 2001
    ....
  42. pmc Microtubule-targeting drugs induce bcl-2 phosphorylation and association with Pin1
    N Pathan
    The Burnham Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Neoplasia 3:550-9. 2001
    ..Bcl-2 could also be coimmunoprecipitated with the kinase Cdc2 in M-phase-arrested cells, suggesting that Cdc2 may be responsible for phosphorylation of Bcl-2 in cells treated ..
  43. ncbi Cell cycle-dependent phosphorylation of Disabled-2 by cdc2
    Junqi He
    Department of Pharmacology, Rollins Research Center, Emory University School of Medicine, Atlanta, GA 30322, USA
    Oncogene 22:4524-30. 2003
    ..Dab2 robustly coimmunoprecipitates from cells with the cyclin-dependent kinase cdc2, and purified cdc2 can phosphorylate purified Dab2 fusion proteins in vitro on multiple sites...
  44. doi DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis
    Lea Marash
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 30:447-59. 2008
    ..Thus, DAP5 is necessary for maintaining cell survival during mitosis by promoting cap-independent translation of at least two prosurvival proteins...
  45. ncbi Identification of plectin as a substrate of p34cdc2 kinase and mapping of a single phosphorylation site
    N Malecz
    Institute of Biochemistry and Molecular Cell Biology, University of Vienna, Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
    J Biol Chem 271:8203-8. 1996
    ..that the major plectin kinase activity in mitotic extracts is related to the cell cycle regulator kinase p34cdc2. Bacterial expression of various truncated plectin mutant proteins comprising different domains of the molecule ..
  46. doi Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis
    Qiang Chen
    The MOE Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio membrane and Membrane Bio engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 18:268-80. 2008
    ..Cyclin B1 is then transported from each kinetochore as microtubule attachment is completed, and this relocalization may redirect the activity of cyclin B1-CDK1 and contribute to inactivation of the spindle assembly checkpoint...
  47. doi Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons
    Zengqiang Yuan
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 319:1665-8. 2008
    ..These findings define a conserved signaling link between Cdk1 and FOXO1 that may have a key role in diverse biological processes, including the degeneration of postmitotic neurons...
  48. pmc Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin
    D S O'Connor
    Departments of Pathology, Dermatology, and Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 97:13103-7. 2000
    ..an inhibitor of apoptosis over-expressed in cancer, physically associates with the cyclin-dependent kinase p34(cdc2) on the mitotic apparatus, and is phosphorylated on Thr(34) by p34(cdc2)-cyclin B1, in vitro and in vivo...
  49. pmc Androgen receptor phosphorylation and stabilization in prostate cancer by cyclin-dependent kinase 1
    Shaoyong Chen
    Hematology Oncology Division, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:15969-74. 2006
    ....
  50. doi A Cullin3-KLHL20 Ubiquitin ligase-dependent pathway targets PML to potentiate HIF-1 signaling and prostate cancer progression
    Wei Chien Yuan
    Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan
    Cancer Cell 20:214-28. 2011
    ..Our study indicates that the KLHL20-mediated PML degradation and HIF-1α autoregulation play key roles in tumor progression...
  51. pmc Cdk1/cyclin B plays a key role in mitotic arrest-induced apoptosis by phosphorylation of Mcl-1, promoting its degradation and freeing Bak from sequestration
    Rong Chu
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Biochem Pharmacol 83:199-206. 2012
    ..These results suggest that Cdk1/cyclin B plays a key role in mitotic arrest-induced apoptosis via Mcl-1 phosphorylation, promoting its degradation and subsequently releasing Bak from sequestration...
  52. pmc p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase
    E Bailly
    Centre de Genetique Moleculaire, CNRS, Gif Yvette, France
    EMBO J 8:3985-95. 1989
    The cdc2+ gene product p34cdc2 is located immunocytochemically in both the nucleus and cytoplasm of human cells. It is uniformly distributed throughout the cytoplasm and is irregularly distributed in the nucleus...
  53. ncbi Interactions of HIV-1 viral protein R with host cell proteins
    Richard Y Zhao
    Department of Pathology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Adv Pharmacol 55:233-60. 2007
  54. ncbi HIV-1 Vpr: a closer look at the multifunctional protein from the structural perspective
    RAMESH C PANDEY
    Department of Biotechnology, Indian Institute of Technology, Chennai, India
    Curr HIV Res 7:114-28. 2009
    ..The inter-relationship between different functions of Vpr is discussed in the context of structure. Based on bioinformatics analysis, we propose new targets for modulating Vpr functions, which need to be validated experimentally...
  55. pmc Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation
    Juan F Linares
    Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Vontz Center for Molecular Studies, 3125 Eden Avenue, Cincinnati, OH 45267, USA
    Mol Cell Biol 31:105-17. 2011
    ..Therefore, p62 emerges as a node for the control of not only cell survival but also cell transit through mitosis...
  56. pmc Cyclin-dependent kinase 1 (CDK1)-mediated phosphorylation of enhancer of zeste 2 (Ezh2) regulates its stability
    Susan C Wu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:28511-9. 2011
    ..Collectively, these results establish a novel function for CDK1-mediated Ezh2 phosphorylation and provide a mechanism by which Ezh2 protein levels can be regulated in cells...
  57. ncbi Mitotic regulation of SIRT2 by cyclin-dependent kinase 1-dependent phosphorylation
    Brian J North
    Gladstone Institute of Virology and Immunology, University of California San Francisco, California 94158, USA
    J Biol Chem 282:19546-55. 2007
    ..Overexpression of SIRT2 mediates a delay in cellular proliferation that is dependent on serine 368 phosphorylation. Furthermore, mutation of serine 368 reduces hyperploidy in cells under mitotic stress due to microtubule poisons...
  58. pmc Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases
    Yakir Moshe
    Unit of Biochemistry, The Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    J Biol Chem 286:16647-57. 2011
    ..Finally, we show that the addition of extracts from S-phase cells to extracts from mitotic cells protects Emi1 from CDK-mediated inactivation...
  59. pmc DNA damage response is suppressed by the high cyclin-dependent kinase 1 activity in mitotic mammalian cells
    Wei Zhang
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 286:35899-905. 2011
    ..Further, the activation of CDK5 in interphase cells impedes the formation of 53BP1 IRIF. Together, these results suggest that the DDR is suppressed by the high CDK1 activity in mitotic mammalian cells...
  60. ncbi Amphiphysin 1 binds the cyclin-dependent kinase (cdk) 5 regulatory subunit p35 and is phosphorylated by cdk5 and cdc2
    S R Floyd
    Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    J Biol Chem 276:8104-10. 2001
    ..Amphiphysin 1 is also phosphorylated by the cdc2/cyclin B kinase complex in the same region and undergoes mitotic phosphorylation in dividing cells...
  61. ncbi SET-related cell division autoantigen-1 (CDA1) arrests cell growth
    Z Chai
    Department of Pathology and Immunology, Monash University Medical School, Prahran, Victoria 3181, Australia
    J Biol Chem 276:33665-74. 2001
    ..We propose that CDA1 is a negative regulator of cell growth and that its activity is regulated by its expression level and phosphorylation...
  62. ncbi Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1
    Leo T O Lee
    Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, PRC
    Ann N Y Acad Sci 1070:393-8. 2006
    ..The first pathway is by changing the expression levels of NFI-C and Sp proteins while the second pathway is by modifying the phosphorylation status of both NFI-C and Sp proteins via Cdk1...
  63. ncbi Differential repression of c-myc and cdc2 gene expression by ERF and PE-1/METS
    Kelly D Hester
    Biomedical Sciences Graduate Program, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Cell Cycle 6:1594-604. 2007
    ..that like PE-1/METS, ERF-mediated repression also requires DP103, and that ERF negatively regulates the c-myc and cdc2 genes...
  64. ncbi Analysis of the potential for HIV-1 Vpr as an anti-cancer agent
    Karrupiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Curr HIV Res 7:144-52. 2009
    ..In this report, we summarize the evidence supporting this hypothesis...
  65. ncbi Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity
    R N Booher
    Onyx Pharmaceuticals, Richmond, California 94806 5206, USA
    J Biol Chem 272:22300-6. 1997
    Activation of the Cdc2.cyclin B kinase is a pivotal step of mitotic initiation. This step is mediated principally by the dephosphorylation of residues threonine 14 (Thr14) and tyrosine 15 (Tyr15) on the Cdc2 catalytic subunit...
  66. ncbi Multiple phosphorylation of chicken protein tyrosine phosphatase 1 and human protein tyrosine phosphatase 1B by casein kinase II and p60c-src in vitro
    E J Jung
    Department of Biochemistry, College of Medicine, Gyeongsang National University, Chinju, Korea
    Biochem Biophys Res Commun 246:238-42. 1998
    ..These results suggest that both CPTP1 and HPTP1B might play an important role in CKII- and p60c-src-induced signal transduction cascades...
  67. ncbi Hypophosphorylation of poly(A) polymerase and increased polyadenylation activity are associated with human immunodeficiency virus type 1 Vpr expression
    Andrew J Mouland
    Department of Medicine, McGill University, Lady Davis Institute Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec, Canada H3T 1E2
    Virology 292:321-30. 2002
    ..Vpr's ability to arrest cells at the G2 phase of the cell cycle is due to the inactivation of p34(cdc2) cyclin B complex, resulting in hypophosphorylation of substrates involved in cell-cycle progression from G2 to ..
  68. ncbi Cell cycle-regulated phosphorylation of hamartin, the product of the tuberous sclerosis complex 1 gene, by cyclin-dependent kinase 1/cyclin B
    Aristotelis Astrinidis
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 278:51372-9. 2003
    ..These findings support a model in which phosphorylation of hamartin regulates the function of the hamartin-tuberin complex during the G2/M phase of the cell cycle...
  69. ncbi Proliferation inhibition of astrocytes, neurons, and non-glial cells by intracellularly expressed human immunodeficiency virus type 1 (HIV-1) Tat protein
    Betty Y Zhou
    Department of Microbiology adn Immunology, Indiana University School of Medicine, Indianapolis, 46202, USA
    Neurosci Lett 359:155-8. 2004
    ..interacted with a number of cell cycle-related proteins including cyclin A, cyclin B, cyclin D3, Cdk2, Cdk4, Cdk1/Cdc2, cdc6, p27, p53, p63, hdlg, and PCNA...
  70. ncbi Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma
    Ma anit Shapira
    Department of Surgery A, Rambam Medical Center, Technion Israel Institute of Technology, Haifa, Israel
    Cancer 100:1615-21. 2004
    ..The essential role of cyclin kinase subunit 1 (Cks1) in Skp2-dependent p27 degradation was recently discovered, but its role in human malignancies is unknown...
  71. ncbi Keratinocyte G2/M growth arrest by 1,25-dihydroxyvitamin D3 is caused by Cdc2 phosphorylation through Wee1 and Myt1 regulation
    Xiuju Dai
    Department of Dermatology, Ehime University School of Medicine, Ehime, Japan
    J Invest Dermatol 122:1356-64. 2004
    ..Interestingly, the levels of phosphorylated Cdc2 were increased between 6 h and 48 h after 1,25[OH]2VD3 treatment, although the expression levels of Cdc2 mRNA and ..
  72. pmc Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling
    Christian Preisinger
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, Germany
    EMBO J 24:753-65. 2005
    ..These findings identify a Plk1-dependent signalling mechanism potentially linking Golgi structure and cell cycle control, but suggest that this may not be a cell cycle checkpoint in the classical sense...
  73. ncbi HIV-1 viral protein R (Vpr) & host cellular responses
    Richard Yuqi Zhao
    Department of Pathology, University of Maryland School of Medicine, 10 South Pine Street, MSTF 600, Baltimore, MD 21201, USA
    Indian J Med Res 121:270-86. 2005
    ..Strategies with potential application for future antiviral therapies directed at suppressing Vpr activities are described...
  74. pmc Cyclin-dependent kinase 1/cyclin B1 phosphorylates varicella-zoster virus IE62 and is incorporated into virions
    Stacey A Leisenfelder
    Department of Microbiology and Immunology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210, USA
    J Virol 82:12116-25. 2008
    ..Thus, IE62 is a substrate for CDK1/cyclin B1, and virions could deliver the active cellular kinase to nondividing cells that normally do not express it...
  75. pmc A conserved phosphorylation site within the forkhead domain of FoxM1B is required for its activation by cyclin-CDK1
    Yi Ju Chen
    Department of Biochemistry and Molecular Genetics, University of Illinois, College of Medicine, Chicago, Illinois 60607, USA
    J Biol Chem 284:30695-707. 2009
    ....
  76. ncbi Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase
    L L Parker
    Department of Physiology, Tufts University School of Medicine, Boston, MA 02111
    Science 257:1955-7. 1992
    ..The wee1+ kinase negatively regulates mitosis by phosphorylating p34cdc2 on tyrosine 15, thereby inactivating the p34cdc2-cyclin B complex...
  77. pmc Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport
    J Pines
    Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186 5800
    J Cell Biol 115:1-17. 1991
    ..Cyclin B1 is associated with p34cdc2 in the cytoplasm...
  78. ncbi Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2
    J Pines
    Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, California 92138
    Cell 58:833-46. 1989
    ..In G2/M phase, cyclin is associated with p34cdc2, the human homolog of the fission yeast gene cdc2+, and this complex has histone H1 kinase activity.
  79. ncbi Activation of p34cdc2 protein kinase by microinjection of human cdc25C into mammalian cells. Requirement for prior phosphorylation of cdc25C by p34cdc2 on sites phosphorylated at mitosis
    U Strausfeld
    Department of Cell Biology, Centre National de la Recherche Scientifique Institut National de la Santé et de la Recherche Médicale, Montpellier, France
    J Biol Chem 269:5989-6000. 1994
    Human cdc25C protein, a specific tyrosine phosphatase that activates the p34cdc2 protein kinase at mitosis, is itself a phosphoprotein that shows increased phosphorylation during the G2-M transition...
  80. ncbi G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently
    P M O'Connor
    Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 268:8298-308. 1993
    ..HN2-induced a protracted delay in G2 phase. This delay correlated with suppression of cyclin B1- and cdc2-kinase activities, and stabilization of hyperphosphorylated-cdc2 in the presence of similar cyclin B1 levels to ..
  81. ncbi Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage
    R Y Poon
    Salk Institute for Biological Studies, La Jolla, California 92037, USA
    J Biol Chem 271:13283-91. 1996
    ..to a low dose of UV irradiation, both p53 and p21 were induced; the protein kinase activities associated with Cdc2, Cdk2, and Cdk4 were inhibited; and there was a good correlation between their inhibition and binding to p21...
  82. ncbi T-loop deletion of CDC2 from breast cancer tissues eliminates binding to cyclin B1 and cyclin-dependent kinase inhibitor p21
    T Ohta
    First Department of Surgery, St Marianna University School of Medicine, Kawasaki, Japan
    Cancer Res 58:1095-8. 1998
    ..We identified from breast cancer tissues a novel variant of human CDC2, termed CDC2deltaT, that lacks 171 nucleotides corresponding to 57 amino acids, which compose most of the T-loop...
  83. pmc Nuclear localization of cyclin B1 controls mitotic entry after DNA damage
    P Jin
    Department of Physiology, University of California, San Francisco, California 94143 0444, USA
    J Cell Biol 141:875-85. 1998
    Mitosis in human cells is initiated by the protein kinase Cdc2-cyclin B1, which is activated at the end of G2 by dephosphorylation of two inhibitory residues, Thr14 and Tyr15...
  84. ncbi Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis
    M Lowe
    Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    Cell 94:783-93. 1998
    ..Here we identify a single serine (Ser-25) in GM130 as the key phosphorylated target and Cdc2 as the responsible kinase...
  85. pmc Overproduction of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 complexes
    F Liu
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    Mol Cell Biol 19:5113-23. 1999
    The Myt1 protein kinase functions to negatively regulate Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15...
  86. ncbi The C-terminal domain of the Cdc2 inhibitory kinase Myt1 interacts with Cdc2 complexes and is required for inhibition of G(2)/M progression
    N J Wells
    Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    J Cell Sci 112:3361-71. 1999
    Activation of Cdc2, is the universal event controlling the onset of mitosis...
  87. ncbi On the concentrations of cyclins and cyclin-dependent kinases in extracts of cultured human cells
    T Arooz
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay
    Biochemistry 39:9494-501. 2000
    ..Using this approach, we formally demonstrated that CDC2 and CDK2 are in excess of their cyclin partners...
  88. pmc A direct role for GRASP65 as a mitotically regulated Golgi stacking factor
    Yanzhuang Wang
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA
    EMBO J 22:3279-90. 2003
    ..GRASP65 is the major phosphorylation target in rat liver Golgi membranes of two mitotic kinases, cdc2-cyclin B and polo-like kinases, which alone will unstack Golgi membranes, generating single cisternae...
  89. ncbi Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints
    Jeremy P H Chow
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
    J Biol Chem 278:40815-28. 2003
    ..In this study, we compared the relative contribution of inhibitory phosphorylation for cyclin A/B1-CDC2 and cyclin A/E-CDK2 complexes...
  90. ncbi Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1
    Rüdiger Neef
    Intracellular Protein Transport, Independent Junior Research Group, Max Planck Institute of Biochemistry, Martinsried, 82152 Germany
    Nat Cell Biol 9:436-44. 2007
    ..The activation state of Cdk1, therefore, controls the switch of Plk1 localization from centrosomes and kinetochores during metaphase, to the central spindle during anaphase...
  91. doi Cdc2 as prognostic marker in stage UICC II colon carcinomas
    Alexander Meyer
    Department of Surgery, Friedrich Alexander University, Erlangen Nuremberg, Germany
    Eur J Cancer 45:1466-73. 2009
    Cyclin-dependent kinase 2 (cdc2) controls the G2-M checkpoint and, therefore, the entrance of cells into mitosis. It might play a crucial role during tumour progression in colon carcinomas (CCA)...
  92. pmc Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis
    David T Terrano
    Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205 7199, USA
    Mol Cell Biol 30:640-56. 2010
    ..Thus, phosphorylation of antiapoptotic Bcl-2 proteins acts as a sensor for CDK1 signal duration and as a functional link coupling mitotic arrest to apoptosis...
  93. pmc Recovery from a DNA-damage-induced G2 arrest requires Cdk-dependent activation of FoxM1
    Monica Alvarez-Fernandez
    Department of Medical Oncology, UMC Utrecht, Universiteitsweg 100, Stratenum 2 118, Utrecht 3584 CG, The Netherlands
    EMBO Rep 11:452-8. 2010
    ..Indeed, we find that cells need to retain sufficient levels of Cdk activity during the DNA-damage response to maintain cellular competence to recover from a DNA-damaging insult...
  94. pmc Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis
    Olivier Gavet
    The Gurdon Institute and Department of Zoology, Tennis Court Road, CB2 1QN Cambridge, UK
    Dev Cell 18:533-43. 2010
    ....
  95. pmc Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited
    Tamara A Potapova
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Mol Biol Cell 22:1191-206. 2011
    ..These findings suggest that the positive feedback in Cdk activation serves to overcome the activity of Cdk-opposing phosphatases and thus sustains forward progression in mitosis...
  96. pmc Overexpressions of Cyclin B1, cdc2, p16 and p53 in human breast cancer: the clinicopathologic correlations and prognostic implications
    Seoung Wan Chae
    Department of Pathology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Jongno gu, Seoul 110 746, Korea
    Yonsei Med J 52:445-53. 2011
    The molecular mechanisms that are responsible for the initiation and progression of breast cancers are largely unknown. This study was to analyze the cyclin B1, cdc2, p53 and p16 tumor suppressor genes in human breast cancer.
  97. ncbi Nuclear localization of CDC25B1 and serine 146 integrity are required for induction of mitosis
    Véronique Baldin
    LBCMCP CNRS UMR5088, Universite Paul Sabatier, 118 route de Narbonne, 31077 Toulouse, France
    J Biol Chem 277:35176-82. 2002
    ..Serine 146 phosphorylation is proposed to be a key event in the regulation of the CDC25B function in the initiation of mammalian mitosis...
  98. pmc Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis
    Huidong Yuan
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
    J Virol 77:2063-70. 2003
    ..Thus, Wee-1 may serve as a key regulator of both HIV type 1 Vpr- and gamma irradiation-mediated apoptosis and possibly serve as a general regulator linking the cell cycle to some pathways of apoptosis...
  99. ncbi Inhibition of cyclin-dependent kinase 1 induces cytokinesis without chromosome segregation in an ECT2 and MgcRacGAP-dependent manner
    Fumihiko Niiya
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 280:36502-9. 2005
    ..Chemical induction of cytokinesis will be a valuable tool to study the initiation mechanism of cytokinesis...
  100. pmc Distinct activation pathways confer cyclin-binding specificity on Cdk1 and Cdk2 in human cells
    Karl A Merrick
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 32:662-72. 2008
    ..Therefore, differential ordering of common activation steps promotes CDK-cyclin specificity, with Cdk7 acting catalytically to enforce fidelity...
  101. pmc Interplay between Cdh1 and JNK activity during the cell cycle
    Gustavo J Gutierrez
    Signal Transduction Program, Sanford Burnham Medical Research Institute, La Jolla, California 92037, USA
    Nat Cell Biol 12:686-95. 2010
    ..This regulatory mechanism between JNK and Cdh1 reveals an important function for JNK during the cell cycle...

Research Grants66

  1. Yun Fai Chris Lau; Fiscal Year: 2014
    ..This project will shed important insights on how the Y-oncogene and X-tumor suppressor operate and how one can interfere or boost their actions respectively to treat prostate cancer. ..
  2. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..Uncovering the function(s) of Cks2 in mammalian meiosis could lead to a better understanding about how Ml is regulated, and help to define the underlying causes of infertility and birth defects in humans. ..
  3. Characterization of JNK in Cell Cycle Control
    ZE apos EV A RONAI; Fiscal Year: 2012
    ..Using biochemical, cellular and genetic models our proposed studies will delineate the requirements and regulation of this newly identified regulatory lobe along the cell cycle and DNA damage response. ..
  4. DAVID OWEN MORGAN; Fiscal Year: 2015
    ....
  5. The CAK Network in Metazoan Cell Cycle Regulation
    Robert P Fisher; Fiscal Year: 2010
    ....
  6. Regulation of M phase exit
    SALLY A KORNBLUTH; Fiscal Year: 2010
    Cdk/Cyclin complexes drive progression through the eukaryotic cell cycle. Entry into mitosis is triggered by Cdk1 (Cdc2)/Cyclin B complexes, while M phase exit requires Cyclin B degradation and dephosphorylation of mitotic phosphoproteins...
  7. Induction and maintenance of mitosis by Cdk1 suppression of Protein phosphatase 1
    ROBERT LEWIS MARGOLIS; Fiscal Year: 2012
    ..This will be a paradigm shift, with the potential of broad implications for understanding the underlying nature of cell cycle mechanisms throughout the cell cycle. ..
  8. Regulation Of A Novel Xenopus Polo-Like Kinase 1 Substrate
    Junjun Liu; Fiscal Year: 2012
    ..The study is expected to reveal a new mechanism of cell cycle control and may also provide a foundation for developing innovative strategies for treating human diseases such as cancer. ..
  9. Control of Mitotic Entry by Regulators of Cdc2
    SALLY A KORNBLUTH; Fiscal Year: 2010
    Entry into mitosis catalyzed by the Cdc2/Cyclin B kinase, which phosphorylates multiple proteins to induce the dramatic cellular rearrangements characteristic of mitosis...
  10. Regulation and function of the Clp 1p protein phosphatase
    Dannel McCollum; Fiscal Year: 2010
    ..3) To test a hypothesis that Clp1 promotes actomyosin ring stability through effects on Cdc15. 4) To determine how Clp1 and the anaphase promoting complex promote Cdc25 inactivation in late mitosis. ..
  11. Herpesvirus protein kinases: Substrate recognition and pathway targeting.
    S Diane Hayward; Fiscal Year: 2013
    ..Viral protein kinase substrate recognition overlaps with that of cellular cdc2/CDK1 kinase but is extended beyond cdc2 sites...
  12. Peter Sicinski; Fiscal Year: 2016
    ..Specific Aim 2. To determine the molecular function of cyclin A-Cdk1 in stem cells. Specific Aim 3. To test the requirement for cyclin A function in Myc-driven breast cancers. ..
  13. Edward T Kipreos; Fiscal Year: 2015
    ..The experiments in this aim will characterize the interaction of cyclin B with CRL2ZYG-11, determine the mechanism through which the degradation is cell cycle regulated, and explore whether the pathway is conserved in mammals. ..
  14. Molecular mechanisms of C/EBP alpha mediated growth arrest
    Nikolai A Timchenko; Fiscal Year: 2013
    ..These complexes occupy the promoters of cyclin D1 and D2. In addition, we identified cdc2 as a kinase which interacts with C/EBP1 at late stages after PH and restores growth inhibitory activity of C/EBP1 ..
  15. Geoffrey I Shapiro; Fiscal Year: 2014
    ..abstract_text> ..
  16. JASON A CHESNEY; Fiscal Year: 2015
    ..This binding pocket then can be used for receptor-based virtual screening to identify novel anti-neoplastic agents. ..
  17. Michele Pagano; Fiscal Year: 2016
    ..Given its critical role, the ubiquitin system is often deregulated in cancer cells. Thus, it is anticipated that the results of the proposed studies will have an impact on both basic science and cancer biology. ..
  18. Stephen L Archer; Fiscal Year: 2016
    ..This is the first study to exploit PAH's reliance on rapid fission to devise novel anti-fission, anti-proliferative therapies for PAH. ..
  19. Investigation of the function and regulation of ERK3
    IRMA SANCHEZ; Fiscal Year: 2009
    ..in the biology of the Golgi, (2) In the regulation of the cell cycle, and (3) Whether the mitotic kinases PLK and cdc2/cyclin B1 regulate ERK3 in vivo...
  20. G2 Role For Cdk2 in Human Cells
    Gregory Enders; Fiscal Year: 2007
    ..that Cyclin dependent kinase 2 (Cdk2) is required for initiation of DNA synthesis in human cells and that Cdk1 (Cdc2) is required for initiation of mitosis...
  21. Timothy C Chambers; Fiscal Year: 2014
    ..At the completion of these experiments, we hope to have gained considerable new insight into the molecular mechanisms of action of this important class of cancer drugs. ..
  22. Functional study of C53 protein as a novel regulator of checkpoint kinases
    Honglin Li; Fiscal Year: 2012
    ..It will lay a foundation for further exploitation of this novel protein as a potential therapeutic target in fighting diseases such as cancer. ..
  23. Systems Architecture And Dynamical Behaviors of the Kinases That Drive M-Phase
    JOSEPH RICHARD POMERENING; Fiscal Year: 2013
    ..This work will yield insights into how the actions of protein networks guide cells to make proper decisions as they proliferate, and may provide significant clues into how cells work to avoid making errors during this process. ..
  24. Dannel McCollum; Fiscal Year: 2016
    ..Our studies on Lats2 targets and activation will have important implications for the processes of tumor suppression and tissue regeneration. ..
  25. Chk1 signaling in the G2 DNA damage checkpoint
    MATTHEW O CONNELL; Fiscal Year: 2009
    ..Chk1 prevents entry into mitosis in response to DNA damage through modulating Cdc2 kinase activity. This pathway and the G2/M cell cycle machinery are ancient in origin and highly conserved...
  26. Andrei Goga; Fiscal Year: 2014
    ..The knowledge gained from these studies will facilitate the development of new therapeutics that target tumor cells with particular genetic changes by precisely inhibiting the cell division cycle. ..
  27. IRON REGULATION OF GENE EXPRESSION
    ELIZABETH ANN LEIBOLD; Fiscal Year: 2013
    ..Increased knowledge of the mechanism by which IRP2 regulates iron balance and cell proliferation is important for developing therapeutics that can be used to target cancer cells and treat iron disorders. ..
  28. James E Ferrell; Fiscal Year: 2016
    ....
  29. Debra J Wolgemuth; Fiscal Year: 2016
    ....
  30. Chemical Genetics of Plasmodium Kinases
    Debopam Chakrabarti; Fiscal Year: 2010
    ....
  31. Targeting of 6-Phosphofructo-2 kinase in Cancer
    Jason Chesney; Fiscal Year: 2009
    ..To determine the effects of cre-mediated single or dual PFKFB3/PFKFB4 genomic deletion on the glucose uptake, Cdk activity and growth of tumors in vivo. ..
  32. Richard H Gomer; Fiscal Year: 2015
    ..hypothesis that the chalone blocks the cell cycle by inhibitin the activity of the G2/ M regulators Cyclin B and Cdc2. Together, this work combining natural products chemistry, physics and mathematical modeling, genetics, cell ..
  33. Michael L Goldberg; Fiscal Year: 2016
    ....
  34. Investigation into Mechanisms of CDK1 in Controlling Telomere Stability
    Weihang Chai; Fiscal Year: 2013
    ....
  35. Peter Sicinski; Fiscal Year: 2016
    ....
  36. Pathways of Therapy-induced Senescence in Cancer
    Daniel Y Wu; Fiscal Year: 2010
    ..cells appear to have bypassed the senescence program by deregulating the expression of the cyclin dependent kinase Cdc2/Cdk1 such that specific inhibition of the Cdc2/Cdk1 kinase both cause escaped cells to re-arrest and block the ..
  37. Robert P Fisher; Fiscal Year: 2016
    ..These studies promise to advance basic understanding of cell-cycle control, and to reveal novel strategies for anti-CDK therapy of human cancer. ..
  38. CONTROL OF CELL DIVISION IN FISSION YEAST
    David Beach; Fiscal Year: 1993
    The cdc2 protein kinase of fission yeast plays a critical role in regulation of the cell cycle and appears to have a homolog in all eukaryotes...
  39. Arsenic Induced Miotic Arrest Associated Apoptosis
    J Christopher States; Fiscal Year: 2009
    ..relieves the arsenite-induced anaphase block by activation of the G2 checkpoint response which inactivates cyclin B/cdc2 and derepresses the mitotic exit network and allow the cells to escape arsenite induced MAAA...
  40. Hironori Funabiki; Fiscal Year: 2016
    ....
  41. STRUCTURE/FUNCTION OF CYCLIN DEPENDENT KINASES
    David Morgan; Fiscal Year: 2002
    ..The major portion of the proposed experiments will address the regulation of the human cyclin-dependent kinase Cdc2. Activation of a complex of Cdc2 and cyclin B1 is required for entry into mitosis...
  42. Determining the mechanism of cyclin B1 association with chromatin
    KATHLEEN LINDAHL PFAFF; Fiscal Year: 2010
    Cyclin B1-Cyclin Dependent Kinase 1 (CDK1) is a fundamental regulator of mitotic entry and exit...
  43. Haojie Huang; Fiscal Year: 2016
    ..Findings from this study will not only enhance our understanding of age-related prostate cancinogenesis, but could also provide new molecular targets to devise strategies for prostate cancer prevention and therapy. ..
  44. Induction of genomic instability by HPV E6 and E7
    Jason J Chen; Fiscal Year: 2012
    ..These studies will shed light on mechanisms by which HPV induces cancer and hold promise for the identification of targets for drug development. ..
  45. CELL CYCLE CHECKPOINT THAT MONITORS MORPHOGENESIS
    Daniel J Lew; Fiscal Year: 2012
    ..The Wee1-family kinases that inhibit Cdc2 are among the targets for these checkpoints both in mammalian cells and in yeast, but the basis for their ..
  46. DNA Replication Control and Its Application to Selective Killing of Cancer Cells
    Wenge Zhu; Fiscal Year: 2012
    ..To accomplish this, currently I am investigating the mechanisms that cause genome instability in cancer cells and its application for cancer therapy, resulting in the application for this award. ..
  47. Cyclic AMP and the Control of the Meiotic Cell Cycle
    Marco Conti; Fiscal Year: 2010
    ..years, we have identified key components in the cAMP signaling cascade that control the activity of MPF (cyclinB/cdc2 complex) in mouse oocytes...
  48. Regulation of Sororin by Cdk1-mediated Phosphorylation.
    William R Taylor; Fiscal Year: 2012
    ..Increased proliferation of fibroblasts and smooth muscle cells has also been implicated in diseases of the cardiovascular system, and knowing how Sororin is regulated may also provide insight into this spectrum of diseases. ..
  49. REGULATION OF MITOSIS IN FISSION YEAST
    Paul Russell; Fiscal Year: 2003
    ..in the mitotic control, which consists of a group of regulatory proteins that converge to modulate the activity of Cdc2, the cyclin-dependent kinase (Cdk) that binds to cyclin-B and catalyzes mitotic events...
  50. Bistability and the Mitotic Trigger
    James E Ferrell; Fiscal Year: 2012
    ..An understanding of the process also promises to shed light on cancer and cancer chemotherapy. ..
  51. Role of CDC14B in mouse oocyte maturation
    KAREN A SCHINDLER; Fiscal Year: 2010
    ..The major goal of this proposal is to determine the role for the CDC14B phosphatase during meiosis in females using the mouse model ..
  52. CDK3-inhibiting anticancer agents
    Donald C Porter; Fiscal Year: 2012
    ..A compound showing safety and efficacy in vivo will be selected for preclinical and clinical development as a new anticancer drug. ..
  53. Examining the cell cycle-dependence of progesterone receptor activity
    LINDSEY STARR TREVINO; Fiscal Year: 2013
    ..Investigation of the factors necessary for PR activity may eventually unveil potential therapeutic targets for breast cancer. ..
  54. Mechanism for Telomeric G-overhang Generation in Human Cells
    Weihang Chai; Fiscal Year: 2011
    ..This proposal aims to yield new insights into telomere maintenance in cancer cells, cell growth control and oncogenesis. It may lead to the development of new anti-cancer therapy. ..
  55. Regulation of Hypoxia-inducible Factor 1 by Cyclin Dependent Kinases
    NOEL ANDREW WARFEL; Fiscal Year: 2013
    ....
  56. Amy E Ikui; Fiscal Year: 2016
    ..The accurate DNA replication control is a critical step to avoid chromosome instability, and it is important to understand since the chromosome instability is a hallmark of tumorigenesis. ..
  57. Kathy K Griendling; Fiscal Year: 2015
    ..the mechanisms responsible for Nox4/Poldip2-induced G2/M arrest, focusing on the cell cycle phosphatases cdc25b and cdc2. In addition, we will use a femoral artery wire injury model in Poldip2 heterozygous mice to create a situation in ..
  58. Regulation of polarized cell growth by GTPases
    Hay Oak Park; Fiscal Year: 2009
    ..abstract_text> ..
  59. Pere Puigserver; Fiscal Year: 2015
    ..Along these lines, we have recently identified Cdc2-like kinase 2 (Clk2) as a novel component downstream of insulin/Akt and functions as part of the hepatic feeding ..
  60. MECHANISM OF DRUG TRANSPORT IN LUNG CANCER CELLS
    Sanjay Awasthi; Fiscal Year: 2011
    ..following three areas: 1) In-vivo studies of efficacy, target specificity, and toxicity, 2) Regulation of Rlip by PKC alpha and tyrosine kinases, and 3) Regulation by signaling proteins including POB1 and cdc2 involved in endocytosis.
  61. Nihal Ahmad; Fiscal Year: 2016
    ....
  62. ELLA R BOSSY-WETZEL; Fiscal Year: 2016
    ....
  63. Mitotic Mechanisms in Alzheimer's Disease
    Blair Leavitt; Fiscal Year: 2006
    ..II) We will complete identification of all known immediate upstream cdc2 regulators in brain, and test their roles in mitotic phosphoepitope formation in our cdc2/cyclin B neuronal line, ..
  64. Regulation of Female Meiosis by the Cdc25b Phosphatase
    Peter Donovan; Fiscal Year: 2006
    ..activation of maturation promoting factor (MPF), the complex of cyclin B and cyclin dependent kinase-1 (CDK1/p34cdc2)...
  65. MEIOTIC CELL CYCLE REGULATION IN REPRODUCTION
    Stuart Ravnik; Fiscal Year: 2002
    ..In fact, the applicant has observed that the peak expression of Cdkl (formerly Cdc2) during meiosis appears not to coincide with peak CycAl expression, suggesting a more specific link between CycAl ..
  66. The Role of Phosphorylation in Regulating Mih1/Cdc25 Activity in Yeast
    VU THUONG THAI; Fiscal Year: 2010
    ..In addition, the mechanisms that generate switch like responses are of fundamental importance to our understanding of the diverse signaling networks that are the target of many known drugs. ..