CD39

Summary

Gene Symbol: CD39
Description: ectonucleoside triphosphate diphosphohydrolase 1
Alias: ATPDase, CD39, NTPDase-1, SPG64, ectonucleoside triphosphate diphosphohydrolase 1, CD39 antigen, ecto-ATP diphosphohydrolase 1, ecto-ATPDase 1, ecto-ATPase 1, ecto-apyrase, lymphoid cell activation antigen
Species: human

Top Publications

  1. pmc Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8
    F Kukulski
    Centre de Recherche en Rhumatologie et Immunologie, Centre hospitalier de l Universite Laval, Ste Foy, Quebec, Canada
    Purinergic Signal 1:193-204. 2005
  2. pmc Ectonucleotide triphosphate diphosphohydrolase-1 (CD39) mediates resistance to occlusive arterial thrombus formation after vascular injury in mice
    Zachary M Huttinger
    Division of Cardiovascular Medicine, The Ohio State University, Columbus, OH 43210 1252, USA
    Am J Pathol 181:322-33. 2012
  3. ncbi The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization
    C R Maliszewski
    Immunex Research and Development Corporation, Seattle, WA 98101
    J Immunol 153:3574-83. 1994
  4. ncbi Identification and characterization of CD39/vascular ATP diphosphohydrolase
    E Kaczmarek
    Sandoz Center for Immunobiology, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 271:33116-22. 1996
  5. ncbi Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression
    Giovanna Borsellino
    Laboratory of Neuroimmunology, Fondazione Santa Lucia, Rome, Italy
    Blood 110:1225-32. 2007
  6. pmc Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression
    Magis Mandapathil
    Department of Pathology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA
    J Immunol Methods 346:55-63. 2009
  7. pmc High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population
    Halima Moncrieffe
    Rheumatology Unit, University College London, London, United Kingdom
    J Immunol 185:134-43. 2010
  8. pmc Expression of CD39 by human peripheral blood CD4+ CD25+ T cells denotes a regulatory memory phenotype
    K M Dwyer
    Immunology Research Centre, Department of Medicine, St Vincent s Hospital, The University of Melbourne, Melbourne, Victoria, Australia
    Am J Transplant 10:2410-20. 2010
  9. pmc Expression of CD39 on FoxP3+ T regulatory cells correlates with progression of HBV infection
    Yan Tang
    Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, People Republic of China
    BMC Immunol 13:17. 2012
  10. ncbi Purification and properties of human placental ATP diphosphohydrolase
    S Christoforidis
    Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece
    Eur J Biochem 234:66-74. 1995

Research Grants

  1. LEUKOREGULATION BY CD39 IN LUNG ISCHEMIA AND INFLAMMATI*
    David Pinsky; Fiscal Year: 2004
  2. optimized Human Apyrase for Bone Cancer Pain Treatment
    Ridong Chen; Fiscal Year: 2013
  3. Potential Therapeutic Applications of Ecto-Nucleotidases in Lithium-induced NDI
    Bellamkonda K Kishore; Fiscal Year: 2010
  4. Thromboregulation in Occlusive Vascular Diseases
    AARON JACOB MARCUS; Fiscal Year: 2012
  5. Production of human CD39 as an antithrombotic agent
    SOONSEOG JEONG; Fiscal Year: 2004
  6. Cell-Cell Interactions in Thrombosis -Request for Extension of Merit Award
    AARON JACOB MARCUS; Fiscal Year: 2013
  7. Jay Rappaport; Fiscal Year: 2015
  8. Bruce Neil Cronstein; Fiscal Year: 2014
  9. Biomedical and Functional Studies of CD39
    AARON JACOB MARCUS; Fiscal Year: 2011
  10. Engineering Inhibitory Antibodies to Ectoenzymes for Cancer Treatment
    Simon C Robson; Fiscal Year: 2013

Detail Information

Publications269 found, 100 shown here

  1. pmc Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8
    F Kukulski
    Centre de Recherche en Rhumatologie et Immunologie, Centre hospitalier de l Universite Laval, Ste Foy, Quebec, Canada
    Purinergic Signal 1:193-204. 2005
    ..Taken together, these data indicate that plasma membrane NTPDases hydrolyze nucleotides in a distinctive manner and may therefore differentially regulate P2 and adenosine receptor signaling...
  2. pmc Ectonucleotide triphosphate diphosphohydrolase-1 (CD39) mediates resistance to occlusive arterial thrombus formation after vascular injury in mice
    Zachary M Huttinger
    Division of Cardiovascular Medicine, The Ohio State University, Columbus, OH 43210 1252, USA
    Am J Pathol 181:322-33. 2012
    ..response to vascular injury, is regulated by hydrolysis of proinflammatory ATP and/or ADP by ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD-1; CD39) to AMP, which then is hydrolyzed by ecto-5'-nucleotidase (CD73) to adenosine...
  3. ncbi The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization
    C R Maliszewski
    Immunex Research and Development Corporation, Seattle, WA 98101
    J Immunol 153:3574-83. 1994
    b>CD39, a 70- to 100-kDa molecule expressed primarily on activated lymphoid cells, was previously shown to mediate B cell homotypic adhesion when ligated with a subset of anti-CD39 mAbs...
  4. ncbi Identification and characterization of CD39/vascular ATP diphosphohydrolase
    E Kaczmarek
    Sandoz Center for Immunobiology, New England Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 271:33116-22. 1996
    Vascular ATP diphosphohydrolase (ATPDase) is a plasma membrane-bound enzyme that hydrolyses extracellular ATP and ADP to AMP...
  5. ncbi Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression
    Giovanna Borsellino
    Laboratory of Neuroimmunology, Fondazione Santa Lucia, Rome, Italy
    Blood 110:1225-32. 2007
    ..indicator of trauma and cell death but can be inactivated by CD39 (nucleoside triphosphate diphosphohydrolase-1 [NTPDase 1]), an ectoenzyme that degrades ATP to AMP...
  6. pmc Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression
    Magis Mandapathil
    Department of Pathology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA
    J Immunol Methods 346:55-63. 2009
    ..b>CD39, a cell surface associated ectonucleotidase, can be used to purify Treg with strong suppressor functions...
  7. pmc High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population
    Halima Moncrieffe
    Rheumatology Unit, University College London, London, United Kingdom
    J Immunol 185:134-43. 2010
    The ectonucleotidase CD39 has recently been described as being highly expressed on regulatory Foxp3(+) CD4 T cells...
  8. pmc Expression of CD39 by human peripheral blood CD4+ CD25+ T cells denotes a regulatory memory phenotype
    K M Dwyer
    Immunology Research Centre, Department of Medicine, St Vincent s Hospital, The University of Melbourne, Melbourne, Victoria, Australia
    Am J Transplant 10:2410-20. 2010
    We have shown that CD39 and CD73 are coexpressed on the surface of murine CD4+ Foxp3+ regulatory T cells (Treg) and generate extracellular adenosine, contributing to Treg immunosuppressive activity...
  9. pmc Expression of CD39 on FoxP3+ T regulatory cells correlates with progression of HBV infection
    Yan Tang
    Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, People Republic of China
    BMC Immunol 13:17. 2012
    ..A newly identified subset of the CD4+Foxp3+ Tregs, the CD39+ Tregs, has been associated with viral infections and autoimmune diseases...
  10. ncbi Purification and properties of human placental ATP diphosphohydrolase
    S Christoforidis
    Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece
    Eur J Biochem 234:66-74. 1995
    ..ATP-DPH activity has been reported to be implicated in the prevention of platelet aggregation, hydrolysing ADP to AMP and thus preventing blood clotting...
  11. ncbi CD39 is an ecto-(Ca2+,Mg2+)-apyrase
    T F Wang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 271:9898-901. 1996
    b>CD39, a 70- to 100-kDa molecule expressed primarily on activated lymphoid cells, was previously identified as a surface marker of Epstein Barr virus (EBV)-transformed B cells...
  12. pmc CD39 modulates endothelial cell activation and apoptosis
    C Goepfert
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Mol Med 6:591-603. 2000
    b>CD39 is the dominant vascular nucleoside triphosphate diphosphohydrolase (NTPDase) that exerts major effects on platelet reactivity by the regulated hydrolysis of extracellular adenine nucleotides...
  13. pmc Central role of Sp1-regulated CD39 in hypoxia/ischemia protection
    Holger K Eltzschig
    Department of Anesthesiology and Perioperative Medicine, University of Colorado Health Sciences Center, Denver, CO, USA
    Blood 113:224-32. 2009
    ..triphosphate/adenosine diphosphate is metabolized to adenosine through a 2-step enzymatic reaction initiated by CD39 (ectonucleoside-triphosphate-diphosphohydrolase-1)...
  14. pmc A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties
    Lishomwa C Ndhlovu
    Division of Experimental Medicine, Department of Medicine, University of California San Francisco, and HIV AIDS Division, San Francisco General Hospital, San Francisco, CA 94110, USA
    Eur J Immunol 40:134-41. 2010
    ..Here, we report that the ectoenzyme CD39/NTPDase1 (ecto-nucleoside triphosphate diphosphohydrolase 1) helps to delineate a novel population of human "..
  15. ncbi Immunolocalization of the ecto-ATPase and ecto-apyrase in chicken gizzard and stomach. Purification and N-terminal sequence of the stomach ecto-apyrase
    S Lewis-Carl
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267 0575, USA
    J Biol Chem 272:23645-52. 1997
    ..data indicate that the stomach 80-kDa protein isolated is an ecto-apyrase and is related to both the chicken liver and oviduct ecto-ATPDase enzymes characterized earlier, as well as to the human lymphoid cell activation antigen, CD39.
  16. pmc Enzymatic properties of an ecto-nucleoside triphosphate diphosphohydrolase from Legionella pneumophila: substrate specificity and requirement for virulence
    Fiona M Sansom
    Department of Microbiology and Immunology and Medicine, University of Melbourne, Parkville, Victoria 3010, Australia
    J Biol Chem 283:12909-18. 2008
    ..pneumophila in eukaryotic cells. Lpg1905 is the first prokaryotic member of the CD39/NTPDase1 family of enzymes, which are characterized by the presence of five apyrase conserved regions and the ..
  17. ncbi Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions
    F Yang
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267 0575, USA
    Biochemistry 40:3943-50. 2001
    ....
  18. ncbi Activities of enzymes that hydrolyze adenine nucleotides in platelets from rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
    Roselia M Spanevello
    Pós Graduação em Bioquímica Toxicológica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitario, Camobi, 97105 900 Santa Maria, RS, Brazil
    Life Sci 80:1109-14. 2007
    The activities of the enzymes NTPDase (EC 3.6.1.5, apyrase, CD39) and 5'-nucleotidase (EC 3.1.3.5, CD73) were analyzed in platelets from rats submitted to demyelination by ethidium bromide (EB) and treated with interferon beta (IFN-beta)...
  19. ncbi Regulation of chicken gizzard ecto-ATPase activity by modulators that affect its oligomerization status
    C C Caldwell
    Department of Chemistry, San Diego State University, California 92182 1030, USA
    Arch Biochem Biophys 387:107-16. 2001
    ..Our findings suggest that, under physiological conditions, the gizzard smooth muscle ecto-ATPase may exhibit a range of activities determined by membrane events that affect the status of oligomerization of the enzyme...
  20. ncbi Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8)
    Takashi Mukasa
    Department of Chemistry and Biochemistry, San Diego State University, San Diego, California 92182 1030, USA
    Biochemistry 44:11160-70. 2005
    ....
  21. ncbi Inhibition of platelet recruitment by endothelial cell CD39/ecto-ADPase: significance for occlusive vascular diseases
    A J Marcus
    Department of Medicine, VA New York Harbor Healthcare System and Weill Medical College of Cornell University, NY 10010, USA
    Ital Heart J 2:824-30. 2001
    ..Experiments initiated in the late 1980's cumulatively indicated that endothelial cell CD39--an ecto-ADPase--was mainly responsible for this phenomenon...
  22. ncbi Transmembrane domains confer different substrate specificities and adenosine diphosphate hydrolysis mechanisms on CD39, CD39L1, and chimeras
    Alison Grinthal
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138
    Biochemistry 41:1947-56. 2002
    ..Previously we showed that H59G substitution changes CD39 from an apyrase to an adenosine diphosphatase (ADPase) in a manner that depends on intact associations of both ..
  23. ncbi Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase
    Aileen F Knowles
    Department of Chemistry, San Diego State University, CA 92182 1030, USA
    Eur J Biochem 269:2373-82. 2002
    ..triphosphate phosphohydrolase in the chicken liver membranes is an ecto-ATP-diphosphohydrolase (ecto- ATPDase) [Caldwell, C., Davis, M.D. & Knowles, A.F. (1999) Arch. Biochem. Biophys. 362, 46-58]...
  24. ncbi Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase
    T M Smith
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267 0575, USA
    Biochemistry 38:5849-57. 1999
    ..Thus, mutation of only two amino acids of an E-type ATPase essentially converts an ecto-apyrase to an ecto-NTPase...
  25. ncbi Site-directed mutagenesis of human endothelial cell ecto-ADPase/soluble CD39: requirement of glutamate 174 and serine 218 for enzyme activity and inhibition of platelet recruitment
    J H Drosopoulos
    Department of Medicine, Division of Hematology and Medical Oncology, VA New York Harbor Healthcare System, New York, New York 10010 5050, USA
    Biochemistry 39:6936-43. 2000
    Endothelial cell CD39/ecto-ADPase plays a major role in vascular homeostasis. It rapidly metabolizes ADP released from stimulated platelets, thereby preventing further platelet activation and recruitment...
  26. ncbi Intravenous apyrase administration reduces arterial thrombosis in a rabbit model of endothelial denudation in vivo
    Andry F Costa
    Programa de pós graduação em Cardiologia, Servico de Cardiologia, Hospital de Clinicas de Porto Alegre, UFRGS, RS, Brazil
    Blood Coagul Fibrinolysis 15:545-51. 2004
    The role of adenine nucleotides on vascular and platelet functions has long been established. Apyrase (CD39) takes part of a family of ecto-enzymes that hydrolyze adenosine diphosphate and adenosine triphosphate...
  27. ncbi CD39-L4 is a secreted human apyrase, specific for the hydrolysis of nucleoside diphosphates
    J J Mulero
    Functional Genomics Department, Hyseq Inc, Sunnyvale, California 94086, USA
    J Biol Chem 274:20064-7. 1999
    The human ecto-apyrase gene family consists of five reported members (CD39, CD39-L1, CD39-L2, CD39-L3, and CD39-L4). The family can be subdivided into two groups by conservation of proposed structural domains...
  28. ncbi Lipopolysaccharide alters ecto-ATP-diphosphohydrolase and causes relocation of its reaction product in experimental intrahepatic cholestasis
    V S Zinchuk
    Department of Anatomy and Cell Biology, Kochi Medical School, Japan
    Cell Tissue Res 304:103-9. 2001
    ..It is suggested that relocation of the reaction product may be a protective mechanism to enable the hepatocytes to withstand the cytokine-induced metabolic perturbations...
  29. ncbi Perindopril augments ecto-ATP diphosphohydrolase activity and enhances endothelial anti-platelet function in human umbilical vein endothelial cells
    Yukio Kishi
    Center for Preventive Medicine, Tokyo Kyosai Hospital, Tokyo, Japan
    J Hypertens 21:1347-53. 2003
    ..Recent clinical trials have demonstrated that angiotensin-converting enzyme inhibitors (ACEIs) reduce thrombotic events by unknown mechanisms in patients with atherosclerotic cardiovascular diseases...
  30. ncbi Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes
    N Nedeljkovic
    Department of General Physiology and Biophysics, Faculty of Biology, University of Belgrade, Studentski trg 3, 11001 Belgrade, Yugoslavia
    Physiol Res 52:797-804. 2003
    Enzymes that hydrolyze extracellular ATP, i.e. ecto-ATPase and ecto-ATP diphosphohydrolase (ATPDase), can be differentiated by ability of the latter to hydrolyze ADP and by slightly different kinetic properties of the two enzymes...
  31. ncbi An ecto-ATPase and an ecto-ATP diphosphohydrolase are expressed in rat brain
    B Kegel
    AK Neurochemie, Biozentrum der J W Goethe Universität, Frankfurt am Main, Germany
    Neuropharmacology 36:1189-200. 1997
    ..Using computer-aided sequence analysis, primary structure and membrane topography are compared with those of other members of the apyrase family...
  32. ncbi Effect of protein-modifying reagents on ecto-apyrase from rat brain
    M R Wink
    , ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Int J Biochem Cell Biol 32:105-13. 2000
    ..Phenylglyoxal and Woodward's reagent also inhibited the GTPase and GDPase activities and this inhibition was prevented by preincubation with these substrates...
  33. pmc GS52 ecto-apyrase plays a critical role during soybean nodulation
    Manjula Govindarajulu
    Donald Danforth Plant Science Center, St Louis, Missouri 63132, USA
    Plant Physiol 149:994-1004. 2009
    ..These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B. japonicum infection process, initiation of nodule primordium development, and subsequent nodule organogenesis in soybean...
  34. ncbi Identification and characterization of a novel hepatic canalicular ATP diphosphohydrolase
    J Sevigny
    Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 275:5640-7. 2000
    We have identified and characterized a novel ATP diphosphohydrolase (ATPDase) with features of E-type ATPases from porcine liver...
  35. ncbi Bacterial expression and characterization of a novel, soluble, calcium-binding, and calcium-activated human nucleotidase
    Deirdre M Murphy
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, P O Box 670575, Cincinnati, Ohio 45267 0575, USA
    Biochemistry 42:2412-21. 2003
    ..This apyrase, which is distinct from the eNTPDases exemplified by the endothelial CD39 (NTPDase1) apyrase, is a 38 kDa monomeric enzyme capable of hydrolyzing a variety of nucleoside di- and ..
  36. ncbi Plasma hemopexin activity in pregnancy and preeclampsia
    Winston W Bakker
    Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen Groningen The Netherlands
    Hypertens Pregnancy 26:227-39. 2007
    ..Plasma hemopexin activity, associated with increased vascular permeability, was evaluated in healthy pregnant and non-pregnant women and in pre-eclamptic women...
  37. doi The impact of purinergic signaling on renal ischemia-reperfusion injury
    Bo Lu
    Immunology Research Centre, St Vincent s Hospital, Melbourne, Victoria, Australia
    Transplantation 86:1707-12. 2008
    ..Ectonucleotidases CD39 and CD73 are integral vascular and immune nucleotidases that regulate extracellular adenosine signaling...
  38. pmc Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39
    Matthew C Hyman
    Department of Molecular and Integrative Physiology, University of Michigan Medical Center, Ann Arbor, USA
    J Clin Invest 119:1136-49. 2009
    Leukocyte and platelet accumulation at sites of cerebral ischemia exacerbate cerebral damage. The ectoenzyme CD39 on the plasmalemma of endothelial cells metabolizes ADP to suppress platelet accumulation in the ischemic brain...
  39. ncbi A bacterial ecto-triphosphate diphosphohydrolase similar to human CD39 is essential for intracellular multiplication of Legionella pneumophila
    Fiona M Sansom
    Australian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, Victoria 3800, Australia
    Cell Microbiol 9:1922-35. 2007
    ..encode ecto-nucleoside triphosphate diphosphohydrolases (ecto-NTPDases) that share sequence similarity with human CD39/NTPDase1...
  40. ncbi ATP release from activated neutrophils occurs via connexin 43 and modulates adenosine-dependent endothelial cell function
    Holger K Eltzschig
    Department of Anesthesiology and Intensive Care Medicine, Tubingen University Hospital, Germany
    Circ Res 99:1100-8. 2006
    ..required PMNs, and studies addressing these metabolic steps revealed that PMN express surface ecto-apyrase (CD39)...
  41. pmc An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
    John F Andersen
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    BMC Genomics 8:102. 2007
    ..Fleas represent a relatively recent group of insects that evolved hematophagy independently of other insect orders...
  42. ncbi Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)
    M Handa
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Biochem Biophys Res Commun 218:916-23. 1996
    ..3-kDa protein on chromosome III, and human CD39, a lymphoid cell activation antigen, also share the conserved ACR regions, but their ability to hydrolyze nucleotides has not been ..
  43. ncbi Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1
    T M Smith
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, 231 Bethesda Avenue, Cincinnati, OH 45267 0575, USA
    Biochim Biophys Acta 1386:65-78. 1998
    ..and rat brain ecto-ATPase cDNAs, human CD39L1 cDNA (putative human ecto-ATPase), and mammalian CD39 (lymphoid cell activation antigen, ecto-apyrase, ATPDase, ATP-diphosphohydrolase) cDNAs...
  44. ncbi Characterization of brain ecto-apyrase: evidence for only one ecto-apyrase (CD39) gene
    T F Wang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Brain Res Mol Brain Res 47:295-302. 1997
    A rat brain cDNA coding for ecto-(Ca,Mg)-apyrase activity was isolated using human CD39 cDNA and functionally expressed in COS-7 cells...
  45. ncbi ATP diphosphohydrolase (NTPDase 1) in rat hippocampal slices and effect of glutamate on the enzyme activity in different phases of development
    Alessandra Nejar Bruno
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, 90035 003, RS, Porto Alegre, Brazil
    Life Sci 71:215-25. 2002
    In the present report we describe an NTPDase 1 (ATP diphosphohydrolase; ecto-apyrase; EC 3.6.1.5) in rat hippocampal slices...
  46. ncbi The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster
    B P Chadwick
    Imperial Cancer Research Fund, London, United Kingdom
    Genomics 50:357-67. 1998
    The human lymphoid cell activation antigen CD39 is a known E-type apyrase that hydrolyzes extracellular ATP and ADP, a function important in homotypic adhesion, platelet aggregation, and removal by activated lymphocytes of the lytic ..
  47. ncbi A human intracellular apyrase-like protein, LALP70, localizes to lysosomal/autophagic vacuoles
    A Biederbick
    UT Southwestern Medical Center, Department of Molecular Biology and Oncology, Dallas, TX 75235 9140, USA
    J Cell Sci 112:2473-84. 1999
    ..Sequence analysis revealed that LALP70 belongs to the apyrase or GDA1/CD39 family and is almost identical to a human uridine diphosphatase, with the exception of nine extra amino acids in ..
  48. ncbi CD39/ectonucleoside triphosphate diphosphohydrolase 1 provides myocardial protection during cardiac ischemia/reperfusion injury
    David Kohler
    Department of Anesthesiology and Intensive Care Medicine, University Hospital, Tubingen, Germany
    Circulation 116:1784-94. 2007
    ..In the present study, we studied ecto-enzymatic ATP/ADP-phosphohydrolysis by select members of the ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) family during myocardial ischemia...
  49. doi Adenosine and inflammation: CD39 and CD73 are critical mediators in LPS-induced PMN trafficking into the lungs
    Jörg Reutershan
    Department of Anesthesiology and Intensive Care Medicine, University of Tubingen, Tubingen, Germany
    FASEB J 23:473-82. 2009
    ..adenosine stems from a coordinated two-step enzymatic conversion of precursor nucleotides via the ecto-apyrase (CD39) and the ecto-5'-nucleotidase (CD73)...
  50. pmc Elucidation of the thromboregulatory role of CD39/ectoapyrase in the ischemic brain
    David J Pinsky
    Division of Cardiology, Department of Medicine, College of Physicians and Surgeons, Columbia University, Presbyterial Hospital 10 Stem, 630 W 168th Street, New York, NY 10032, USA
    J Clin Invest 109:1031-40. 2002
    Endothelial CD39 metabolizes ADP released from activated platelets...
  51. ncbi Contribution of E-NTPDase1 (CD39) to renal protection from ischemia-reperfusion injury
    Almut Grenz
    Department of Pharmacology and Toxicology, Tubingen University Hospital, Tubingen, Germany
    FASEB J 21:2863-73. 2007
    ..responses of E-NTPDases (E-NTPDase1-3, and 8) to renal IP, and found a robust and selective induction of E-NTPDase1/CD39 transcript and protein...
  52. ncbi Identification of ectonucleotidases CD39 and CD73 in innate protection during acute lung injury
    Tobias Eckle
    Department of Anesthesiology and Intensive Care Medicine, Center for Biomedical Research, Tubingen University Hospital, Hoppe Seyler Strasse 3, D 72076 Tubingen, Germany
    J Immunol 178:8127-37. 2007
    ..Because ectoapyrase (CD39) and ecto-5'-nucleotidase (CD73) are rate limiting for extracellular adenosine generation, we examined their ..
  53. doi Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis
    Irena Lavrnja
    Department of Neurobiology, Institute for Biological Research Sinisa Stankovic University of Belgrade, Bulevar Despota Stevana 142, 11000 Belgrade, Serbia
    Neurochem Int 55:193-8. 2009
    The aim of the present study was to analyze the activities of extracellular purine metabolizing enzymes, CD39 (apyrase, EC 3.6.1.5) and CD73 (ecto-5' nucleotidase, EC 3.1.3.5) in experimental autoimmune encephalomyelitis (EAE)...
  54. pmc SP1-dependent induction of CD39 facilitates hepatic ischemic preconditioning
    Melanie L Hart
    Department of Anesthesiology and Intensive Care Medicine, University of Tubingen, Tubingen, Germany
    J Immunol 184:4017-24. 2010
    ..we hypothesized that phosphohydrolysis of ATP/ADP via the ectonucleoside triphosphate diphosphohydrolase-1 (CD39), conversion of ATP/ADP to AMP, mediates IP-dependent liver protection...
  55. ncbi A yeast Golgi E-type ATPase with an unusual membrane topology
    X Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 274:32704-11. 1999
    ..this study, we show that a gene of Saccharomyces cerevisiae, identified by similarity to that of animal ectoapyrase CD39, codes for a new member of the E-type ATPase family (Apy1p)...
  56. ncbi Regulation of yeast ectoapyrase ynd1p activity by activator subunit Vma13p of vacuolar H+-ATPase
    X Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 275:35592-9. 2000
    b>CD39-like ectoapyrases are involved in protein and lipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae...
  57. ncbi Characterization of Schistosoma mansoni ATPDase2 gene, a novel apyrase family member
    Julio Levano-Garcia
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, 05508 900 Sao Paulo, SP, Brazil
    Biochem Biophys Res Commun 352:384-9. 2007
    ..In this work, we describe the cloning and characterization of SmATPDase2, a novel CD39-like ATP diphosphohydrolase gene in S. mansoni...
  58. ncbi Establishment and characterization of a novel ALL-L3 cell line (BALM-18): induction of apoptosis by anti-IgM and inhibition of apoptosis by bone marrow stroma cells
    Y Matsuo
    Fujisaki Cell Center, Hayashibara Biochemical Labs Inc, Okayama, Japan
    Leuk Res 23:559-68. 1999
    ..the primary leukemia cells, BALM-18 showed an immunophenotype of Burkitt's lymphoma group I [CD10+, CD20+, CD23-, CD39-, CD77+] and carried the t(8;14)(q24;q32) chromosomal abnormality which is highly associated with ALL-L3 and ..
  59. ncbi HIV infection is associated with increased NTPDase activity that correlates with CD39-positive lymphocytes
    Daniela B R Leal
    Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600 Anexo, 90035 003, Porto Alegre, RS, Brazil
    Biochim Biophys Acta 1746:129-34. 2005
    ..6.1.5) activity in lymphocytes of HIV-positive patients, confirmed by an enhanced CD39 expression on its surface...
  60. ncbi Molecular cloning and characterization of expressed human ecto-nucleoside triphosphate diphosphohydrolase 8 (E-NTPDase 8) and its soluble extracellular domain
    Aileen F Knowles
    Department of Chemistry and Biochemistry, San Diego State University, San Diego, California 92182 1030, USA
    Biochemistry 45:7323-33. 2006
    ..Sequence analysis indicated that it is most closely related to a chicken ecto-ATPDase previously cloned in our laboratory [Knowles et al. (2002) Eur. J. Biochem...
  61. pmc Characterization of regulatory T cells identified as CD4(+)CD25(high)CD39(+) in patients with active tuberculosis
    T Chiacchio
    Translational Research Unit, National Institute for Infectious Diseases INMI L Spallanzani, IRCCS, Rome, Italy
    Clin Exp Immunol 156:463-70. 2009
    ..Recently CD39 has been shown to be an accurate marker for T(reg) detection...
  62. pmc Vascular stasis, intestinal hemorrhage, and heightened vascular permeability complicate acute portal hypertension in cd39-null mice
    Xiaofeng Sun
    Department of Medicine, Division of Gastroenterology and Liver Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Am J Physiol Gastrointest Liver Physiol 297:G306-11. 2009
    ..b>CD39/ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase1) is the major vascular ectonucleotidase that hydrolyzes ..
  63. pmc CD39+ regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism
    Zhi Jian Ye
    Department of Respiratory Diseases, Key Laboratory of Pulmonary Diseases of Health Ministry, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Huazhong, China
    Respir Res 12:77. 2011
    ....
  64. pmc Phenotypic complexity of T regulatory subsets in patients with B-chronic lymphocytic leukemia
    Angelique Biancotto
    Center for Human Immunology, Autoimmunity and Inflammation, National Heart Lung and Blood Institute, Bethesda, MD, USA
    Mod Pathol 25:246-59. 2012
    ..A population of CCR7(+)CD39(+) T(regs) was significantly associated with chronic lymphocytic leukemia...
  65. pmc Metabolism of circulating ADP in the bloodstream is mediated via integrated actions of soluble adenylate kinase-1 and NTPDase1/CD39 activities
    Gennady G Yegutkin
    MediCity Research Laboratory, University of Turku, Tykistokatu 6A, 20520 Turku, Finland
    FASEB J 26:3875-83. 2012
    ..presence of another nucleotide-converting enzyme, nucleoside triphosphate diphosphohydrolase-1 (NTPDase1, known as CD39), was ascertained by the relative deficiency of serum from CD39-null mice to dephosphorylate [(3)H]ADP and [γ-(32)..
  66. doi CD39 is involved in mediating suppression by Mycobacterium bovis BCG-activated human CD8(+) CD39(+) regulatory T cells
    Mardi C Boer
    Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Eur J Immunol 43:1925-32. 2013
    ..Here, we describe for the first time the expression of CD39 (E-NTPDase1) on Mycobacterium-activated human CD8(+) T cells...
  67. pmc Trichostatin A promotes the generation and suppressive functions of regulatory T cells
    Cristian Doñas
    Departamento de Ciencias Biologicas, Universidad Andres Bello, Republica 275, Santiago, Chile
    Clin Dev Immunol 2013:679804. 2013
    ..suppressive activities, which could potentially be explained by a mechanism involving the ectonucleotidases CD39 and CD73...
  68. doi IL-7 modulates in vitro and in vivo human memory T regulatory cell functions through the CD39/ATP axis
    Mehwish Younas
    INSERM U955, Institut Mondor de Recherche Biomedicale, 94010 Creteil, France
    J Immunol 191:3161-8. 2013
    ..We provide evidence that IL-7 exerts a synergistic effect through downmodulation of the ectoenzyme CD39, which converts ATP to ADP/AMP, and an increase in ATP receptor P2X7...
  69. pmc Immunological monitoring of extracorporeal photopheresis after heart transplantation
    M T Dieterlen
    Department of Cardiac Surgery, Heart Center, University Hospital Leipzig, Leipzig, Germany
    Clin Exp Immunol 176:120-8. 2014
    ..b>CD39-positive T(regs) increased during ECP treatment compared to HTxC...
  70. doi Role of adaptive and innate immune cells in chronic fatigue syndrome/myalgic encephalomyelitis
    Ekua Weba Brenu
    School of Medical Science, Griffith University, Gold Coast, QLD 4215, Australia
    Int Immunol 26:233-42. 2014
    ..Significant changes were observed in B-cell subsets, Tregs, CD4(+)CD73(+)CD39(+) T cells, cytotoxic activity, granzyme B, neutrophil antigens, TNF-α and IFN-γ in the CFS/ME patients in ..
  71. doi A commensal bacterial product elicits and modulates migratory capacity of CD39(+) CD4 T regulatory subsets in the suppression of neuroinflammation
    Yan Wang
    Department of Microbiology and Immunology Geisel School of Medicine Dartmouth College Hanover, NH USA
    Gut Microbes 5:552-61. 2014
    ..This protection is dependent upon the expansion of immune-regulatory CD4 T cells (Treg) expressing CD39, an ectonucleotidase...
  72. pmc Generation, cryopreservation, function and in vivo persistence of ex vivo expanded cynomolgus monkey regulatory T cells
    Hao Guo
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cell Immunol 295:19-28. 2015
    ..The expanded Treg maintained their expression of Treg signature markers, CD25, CD27, CD39, Foxp3, Helios, and CTLA-4, as well as CXCR3, which plays an important role in T cell migration to sites of ..
  73. pmc Hypoxia-induced inflammation and purinergic signaling in cross clamping the human aorta
    Juho Jalkanen
    Department of Vascular Surgery, Turku University and Turku University Hospital, Hämeenkatu 11, 20521 Turku, Finland
    Springerplus 5:2. 2016
    ..48 different cytokines, chemokines and growth factors were analyzed, in addition to circulating levels of ATP, ADP, CD39, CD73 and HIF-1α, and compared between the groups...
  74. pmc Ectonucleotidase activity and immunosuppression in astrocyte-CD4 T cell bidirectional signaling
    Fabia Filipello
    Laboratory of Pharmacology and Brain Pathology, Humanitas Clinical and Research Center, Rozzano, Italy
    Oncotarget 7:5143-56. 2016
    ..Here we show that astrocytes promote the expression and enzymatic activity of CD39 and CD73 ectonucleotidases in recently activated CD4 cells by a contact dependent mechanism that is independent of ..
  75. pmc The effect of cellular isolation and cryopreservation on the expression of markers identifying subsets of regulatory T cells
    Weiying Zhang
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA Electronic address
    J Immunol Methods 431:31-7. 2016
    ..However, it is unknown if PBMC isolation and cryopreservation affect the expression of CD120b and CD39, markers that identify specific subsets of Tregs.
  76. pmc Enrichment of Inflammatory IL-17 and TNF-α Secreting CD4(+) T Cells within Colorectal Tumors despite the Presence of Elevated CD39(+) T Regulatory Cells and Increased Expression of the Immune Checkpoint Molecule, PD-1
    Margaret R Dunne
    School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland School of Medicine, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland
    Front Oncol 6:50. 2016
    ..CD4(+)CD25(+)CD127(lo) and CD4(+)CD25(+)CD127(lo)FoxP3(+)CD39(+) Treg cells were enriched in colorectal tumors...
  77. doi Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1
    John W Bullen
    Vascular Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Sci Signal 9:ra56. 2016
    ..Furthermore, increased cAMP concentrations enhanced the expression of HIF target genes encoding CD39 and CD73, which are enzymes that convert extracellular adenosine 5'-triphosphate to adenosine, a molecule that ..
  78. ncbi P2X7 Receptor Signaling Contributes to Sepsis-Associated Brain Dysfunction
    Luiz Eduardo Baggio Savio
    Laboratory of Immunophysiology, Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    Mol Neurobiol . 2016
    ..We also determined how this process is regulated by the ectonucleotidase CD39, a scavenger of extracellular nucleotides...
  79. ncbi Production of heterozygous alpha 1,3-galactosyltransferase (GGTA1) knock-out transgenic miniature pigs expressing human CD39
    Kimyung Choi
    Optipharm Inc, 63, Osongsaengmyeong 6 ro, Osong eup, Heungdeok Gu, Cheongju Si, Chungcheongbuk Do, Republic of Korea
    Transgenic Res . 2016
    ..Human CD39 rapidly hydrolyzes ATP and ADP to AMP; AMP is hydrolyzed by ecto-5'-nucleotidase (CD73) to adenosine, an anti-..
  80. doi Protective role of hypoxia-inducible factor-1α-dependent CD39 and CD73 in fulminant acute liver failure
    Eunyoung Tak
    Asan Institute for Life Sciences and Asan Minnesota Institute for Innovating Transplantation, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
    Toxicol Appl Pharmacol 314:72-81. 2017
    ..Although human ectonucleotide triphosphate diphosphohydrolase-1, E-NTPDase1 (CD39) and ecto-5'-nucleotidase, Ecto5'NTase (CD73) are known to protect tissues from ALF, the expression and function of ..
  81. ncbi Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL
    J Stockl
    Institute of Immunology, University of Vienna, Medical School, Vienna, Austria
    J Immunol 167:2724-33. 2001
    ..In both cases, recognition was mediated by the TCR. This MHC-independent recognition is target cell type restricted and critically involves in our model direct recognition of the ectonucleotidase family surface molecule CD39 by the CTL.
  82. ncbi A novel ALL-L3 cell line, BALM-25, expressing both immunoglobulin light chains
    Yoshinobu Matsuo
    Fujisaki Cell Center, Hayashibara Biochemical Labs Inc, Okayama 702 8006, Japan
    Leuk Lymphoma 45:575-81. 2004
    ..including expression of cell surface and cytoplasmic immunoglobulin (Ig) chains, CD10, CD19, CD20, CD38, CD39, CD40, CD71, NU-B1 and HLA class II. T-cell and myeloid associated antigens tested were negative except CD5...
  83. doi Tubuloglomerular feedback and renin secretion in NTPDase1/CD39-deficient mice
    Mona Oppermann
    National Institute of Digestive and Diabetes and Kidney Diseases, National Institute of Health, 10 Center Drive MSC 1370, Bethesda, MD 20892, USA
    Am J Physiol Renal Physiol 294:F965-70. 2008
    ..can be traced back to ATP dephosphorylation, experiments were performed in mice with a deficiency in NTPDase1/CD39, an ecto-ATPase catalyzing the formation of AMP from ATP and ADP...
  84. doi Foxp3+CD4+ T cell-mediated immunosuppression involves extracellular nucleotide catabolism
    Margaret S Bynoe
    College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA
    Trends Immunol 29:99-102. 2008
    ..Novel studies now establish that, through the generation of the immunosuppressive factor adenosine, the ectoenzymes CD39 and CD73 are important contributors to the regulatory activity of Foxp3(+)CD4(+) T cells.
  85. pmc CD39 is incorporated into plasma microparticles where it maintains functional properties and impacts endothelial activation
    Yara Banz
    Liver Center, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA 02215, USA
    Br J Haematol 142:627-37. 2008
    ..The ectonucleotidase CD39/ecto-nucleoside triphosphate diphosphohydrolase1 (E-NTPDase1) modulates purinergic signalling through pericellular ..
  86. doi Ectonucleotidases as regulators of purinergic signaling in thrombosis, inflammation, and immunity
    Silvia Deaglio
    Department of Genetics, Biology, and Biochemistry, University of Turin and Human Genetics Foundation, Italy
    Adv Pharmacol 61:301-32. 2011
    ..Within the vasculature, spatial and temporal expression of CD39 nucleoside triphosphate diphosphohydrolase (NTPDase) family members together with CD73 ecto-5'-nucleotidase control ..
  87. pmc Transgenic over expression of ectonucleotide triphosphate diphosphohydrolase-1 protects against murine myocardial ischemic injury
    Ming Cai
    Division of Cardiovascular Medicine and Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA
    J Mol Cell Cardiol 51:927-35. 2011
    Modulation of purinergic signaling is critical to myocardial homeostasis. Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD-1; CD39) which converts the proinflammatory molecules ATP or ADP to AMP is a key regulator of purinergic ..
  88. doi Leishmania (Viannia) braziliensis nucleoside triphosphate diphosphohydrolase (NTPDase 1): localization and in vitro inhibition of promastigotes growth by polyclonal antibodies
    Gabriane Nascimento Porcino
    Departamento de Bioquimica, Pós Graduação em Imunologia e DIP Genética e Biotecnologia, Instituto de Ciencias Biologicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
    Exp Parasitol 132:293-9. 2012
    ..Viannia) braziliensis promastigotes (Lb), and an antigenic conserved domain (r82-121) from the specific NTPDase 1 isoform was identified...
  89. pmc The CD39-adenosinergic axis in the pathogenesis of immune and nonimmune diabetes
    Joanne S J Chia
    Immunology Research Centre, St Vincent s Hospital Melbourne, Fitzroy 3065, Australia
    J Biomed Biotechnol 2012:320495. 2012
    ..In this paper, we discuss the role of the CD39-adenosinergic axis in the pathogenesis of both T1D and T2D, with particular emphasis on the role of CD39 and CD73.
  90. pmc Rolofylline, an adenosine A1 receptor antagonist, inhibits osteoclast differentiation as an inverse agonist
    Wenjie He
    New York University School of Medicine, New York, NY, USA
    Br J Pharmacol 170:1167-76. 2013
    ..may be generated by hydrolysis of extracellular nucleotides by ectonucleotidases, including ectonucleoside triphosphate diphosphohydrolase 1 (CD39), ecto-5'-nucleotidase (CD73), nucleotide pyrophosphatase phosphodiesterase 1 (NPP-1) ..
  91. doi CD39/CD73 and the imbalance of Th17 cells and regulatory T cells in allergic asthma
    Lin Lin Wang
    Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P R China
    Mol Med Rep 8:1432-8. 2013
    ..b>CD39 is expressed on the surface of a subset of naturally occurring human Tregs that are important in constraining ..
  92. pmc Ecto-5'-nucleotidase (CD73) regulates host inflammatory responses and exacerbates murine salmonellosis
    M Samiul Alam
    Immunobiology Branch, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD 20708
    Sci Rep 4:4486. 2014
    ..Adenosine, an important immune regulator of inflammation, limits tissue damage during infection. CD39 (nucleoside triphosphate dephosphorylase) combined with ecto-5'-nucleotidase (CD73) metabolizes ATP to adenosine...
  93. pmc Purinergic signalling and immune cells
    Geoffrey Burnstock
    Autonomic Neuroscience Centre, University College Medical School, Rowland Hill Street, London, NW3 2PF, UK
    Purinergic Signal 10:529-64. 2014
    ..Furthermore, ATP is rapidly degraded into adenosine by ectonucleotidases such as CD39 and CD73, and adenosine exerts additional regulatory effects through its own receptors...
  94. pmc CD39 deficiency in murine liver allografts promotes inflammatory injury and immune-mediated rejection
    Osamu Yoshida
    Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA Electronic address
    Transpl Immunol 32:76-83. 2015
    ..It acts as a damage-associated molecule pattern to stimulate innate immune cells. The ectonucleotidase CD39 regulates immune activation by hydrolysis of extracellular ATP...
  95. pmc The Yin and Yang aspects of IL-27 in induction of cancer-specific T-cell responses and immunotherapy
    Ming Song Li
    Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China
    Immunotherapy 7:191-200. 2015
    ..However, animal studies also suggest that IL-27 induces molecular pathways such as IL-10, PD-L1 and CD39, which may downregulate tumor-specific T-cell responses...
  96. doi Extracellular ATP metabolism on vascular endothelial cells: A pathway with pro-thrombotic and anti-thrombotic molecules
    Eduardo Fuentes
    Department of Clinical Biochemistry and Immunohaematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging PIEI ES, Universidad de Talca, Talca, Chile Centro de Estudios en Alimentos Procesados CEAP, CONICYT Regional, Gore Maule, R09I2001 Talca, Chile Electronic address
    Vascul Pharmacol 75:1-6. 2015
    ..contributes to the metabolism and interconversion of extracellular adenine nucleotides via ecto-ATPase/ADPase (CD39) and ecto-5'nucleotidase (CD73) activities...
  97. pmc Dual roles of the adenosine A2a receptor in autoimmune neuroinflammation
    J Ingwersen
    Department of Neurology, Medical Faculty, Heinrich Heine University Dusseldorf, Moorenstr 5, 40225, Dusseldorf, Germany
    J Neuroinflammation 13:48. 2016
    ..of extracellular ATP upon cell death, which can be degraded to adenosine by membrane-bound ecto-enzymes like CD39 and CD73...
  98. doi Divergent regulatory roles of extracellular ATP in the degranulation response of mouse bone marrow-derived mast cells
    Kazuki Yoshida
    Laboratory of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, 60 Nakaorui machi, Takasaki shi, Gunma 370 0033, Japan
    Int Immunopharmacol 43:99-107. 2016
    ..Moreover, BMMCs highly expressed ecto-nucleotidase CD39, but not ecto-5'-nucleotidase (CD73), and were therefore unable to directly convert ATP to adenosine...
  99. ncbi Subepithelial B cells in the human palatine tonsil. I. Morphologic, cytochemical and phenotypic characterization
    M Dono
    Servizio di Immunologia Clinica, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy
    Eur J Immunol 26:2035-42. 1996
    ..surface phenotype (IgM+, IgD+, CD23-, CD38+/-, CD10-, CD44+) that was different from that of FM (IgM+, IgD+, CD23+, CD39+, CD38-, CD10-, CD44+2) and of germinal center (GC) (CD23-, CD39-, CD38+, CD10+, CD44+/-, IgG+) B cells isolated ..
  100. ncbi Complementary DNA cloning and sequencing of the chicken muscle ecto-ATPase. Homology with the lymphoid cell activation antigen CD39
    T L Kirley
    Department of Pharmacology and Cell Biophysics, College of Medicine, University of Cincinnati, Ohio 45267 0575, USA
    J Biol Chem 272:1076-81. 1997
    ..However, the most striking homology observed was to the human and mouse lymphoid cell activation antigen 39 (CD39), a molecule now known to have apyrase activity...
  101. ncbi Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases
    S Berchtold
    Department of Dermatology, University of Erlangen, Germany
    FEBS Lett 458:424-8. 1999
    ..DC expressed the ecto-apyrase CD39 and the ecto-5'-nucleotidase CD73 as demonstrated by RT-PCR...

Research Grants52

  1. LEUKOREGULATION BY CD39 IN LUNG ISCHEMIA AND INFLAMMATI*
    David Pinsky; Fiscal Year: 2004
    ..We hypothesized that endothelial cell (EC) expression of a transmembrane protein, CD39 (ectoapyrase), which metabolizes ATP and ADP in the releasate of activated cells, provides a critical mechanism by ..
  2. optimized Human Apyrase for Bone Cancer Pain Treatment
    Ridong Chen; Fiscal Year: 2013
    ..APT102 is a proprietary, optimized human apyrase of the CD39 family...
  3. Potential Therapeutic Applications of Ecto-Nucleotidases in Lithium-induced NDI
    Bellamkonda K Kishore; Fiscal Year: 2010
    ..To achieve this goal, we will use CD39 (NTPDase1, an ecto-nucleotidase) over expressing transgenic mouse model;and (ii) to determine whether ..
  4. Thromboregulation in Occlusive Vascular Diseases
    AARON JACOB MARCUS; Fiscal Year: 2012
    ..The broad, long term objectives and specific aims of this proposal are to bring the ecto-enzyme CD39/NTPDase1 to clinical fruition via the in vitro and ex vivo studies described herein...
  5. Production of human CD39 as an antithrombotic agent
    SOONSEOG JEONG; Fiscal Year: 2004
    ..abstract_text> ..
  6. Cell-Cell Interactions in Thrombosis -Request for Extension of Merit Award
    AARON JACOB MARCUS; Fiscal Year: 2013
    ..This unrespon- siveness is due to endothelial NTPDasel/CD39/ecto-ADPase, which rapidly metabolizes ATP and ADP released from activated platelets...
  7. Jay Rappaport; Fiscal Year: 2015
    ..Microglia in CNS, similar to an action of regulatory T cells, express ectonucleotidases CD39 and CD73, which convert ATP and ADP to AMP (via CD39), with further metabolism to adenosine (via CD73), providing a ..
  8. Bruce Neil Cronstein; Fiscal Year: 2014
    ..using animals deficient in the enzymes that convert extracellular adenine nucleotides to adenosine (NPP-1, TNAP, CD39 and CD73)...
  9. Biomedical and Functional Studies of CD39
    AARON JACOB MARCUS; Fiscal Year: 2011
    ..This unresponsiveness is due to endothelial NTPDase1/CD39/ecto-ADPase, which rapidly metabolizes ATP and ADP released from activated platelets...
  10. Engineering Inhibitory Antibodies to Ectoenzymes for Cancer Treatment
    Simon C Robson; Fiscal Year: 2013
    ..In particular, we have shown that the cell surface ectonucleotidase CD39 hydrolyzes extracellular nucleotides to produce adenosine, which strongly suppresses anti-tumor immunity and ..
  11. Peter B Ernst; Fiscal Year: 2014
    ..Adenosine accumulates in inflamed or hypoxic tissues largely due to CD39 mediating the dephosphorylation of ATP to ADP then to 5'-AMP while CD73 catalyzes the terminal reaction to ..
  12. Purinergic receptors in inflammation
    Wolfgang G Junger; Fiscal Year: 2012
    ..A3 adenosine receptors are recruited to the leading edge where adenosine is generated by CD39/E- NTPDase1 and alkaline phosphatase (ALP)...
  13. Hypoxia and Inflammation at the Vascular Interface
    Sean P Colgan; Fiscal Year: 2013
    ..Initial insight gained from global transcriptional arrays identified the prominent regulation of surface enzymes (CD39, CD73, adenosine deaminase), nucleotide transporters (ENT1 and ENT2) and Ado receptors (esp...
  14. Gyorgy Hasko; Fiscal Year: 2016
    ..of its precursors ATP and ADP, and their sequential cell surface phosphohydrolysis to AMP by ectonucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1, CD39) and then to adenosine by ecto-5'-nucleotidase (Ecto5'Ntase, CD73)..
  15. Alan Colm Moss; Fiscal Year: 2015
    ..In particular, this proposal will study a group of enzymes (CD39, CD73) and receptors that convert harmful products of tissue damage (ATP, ADP) to immuno- suppressive molecules (..
  16. David M Mosser; Fiscal Year: 2016
    ..We will determine the role that macrophage ectoenzymes, CD39 and CD73, play in promoting the conversion of ATP to adenosine by engineering macrophages to overexpress or lack ..
  17. Thrombus Formation In Vivo
    Bruce Furie; Fiscal Year: 2012
    ..In Project #3 entitled CD39 AND ITS ROLE IN THROMBOGENESIS, Dr...
  18. Extracellular Adenosine During Ventilator Induced Lung Injury
    Holger K Eltzschig; Fiscal Year: 2013
    ..at inflammatory tissue sites are metabolized to adenosine by surface expressed ecto-nucleotidases (CD73 and CD39)...
  19. GERARD ANTHONY LUTTY; Fiscal Year: 2014
    ..We have cultured human retinal angioblasts (CXCR4+, cKit+, CD39+) from avascular neonatal dog and fetal human retina...
  20. CD39-mediated cardiovascular protection
    Richard J Gumina; Fiscal Year: 2010
    ..One such recently recognized cardiovascular protective protein is ectonucleoside triphosphate diphosphohydrolase 1 (CD39)...
  21. Non-Human primate models for human xenotransplantation
    Mary K Kearns Jonker; Fiscal Year: 2010
    ..we plan to examine in our study have been bred on a gal knockout background and have additional transgenes such as CD39 to prevent thrombosis...
  22. GALT mediated protection against CNS demyelination: Role of commensal bacteria
    Lloyd H Kasper; Fiscal Year: 2012
    ..cord and CNS associated lymphoid tissues and 3) to determine the requirement of TLR2 in the induction of CD39+/- Tregs and B cells by PSA in the CNS and its associated lymphoid tissue...
  23. "Mechanisms of IL-35 Protection Against Arthritis"
    David W Pascual; Fiscal Year: 2011
    ..be IL-10-dependent, produced by heterogeneous regulatory T cell subsets, including CD25+ CD4+ T cells, as well as CD39+ CD4+ T cells...
  24. Thromboregulatory strategies to prolong xenograft survival.
    Simon Robson; Fiscal Year: 2009
    ..We will evaluate transgenic approaches to over-express CD39, a key thromboregulatory protein and/or thrombomodulin in pigs...
  25. Elliot Chaikof; Fiscal Year: 2014
    ..2) Determine the effectiveness of CD39/CD73 targeted to sites of vessel wall injury in limiting purinergic mediated inflammatory pathways that contribute ..
  26. Elliot Chaikof; Fiscal Year: 2016
    ..g. TM, heparin) or purinergic (e.g. CD39) pathways...
  27. STRUCTURE AND FUNCTION OF ECTO ADPASE/CD39
    Min Lu; Fiscal Year: 2005
    ..This observation culminated in our characterization of endothelial cell CD39/ecto-ADPase as the prime thromboregulator...
  28. Amanda S Macleod; Fiscal Year: 2016
    ..T cells and their capacity to induce repair and defense genes or apoptosis in keratinocytes;2) examining whether CD39+ cells impair skin-resident T cell function and 3) elucidating the efficacy of purinergic receptor directed ..
  29. Pharmacology of Antirheumatic Drugs
    Bruce Cronstein; Fiscal Year: 2007
    ..release and suppress inflammation in animals deficient in nucleoside triphosphate dephosphorylase (NTPDase, CD39) and ecto-5'Nucleotidase (ecto-5'NTase, CD73)...
  30. T CELL DEPENDENT TERMINAL MATURATION OF LANGERHANS CELLS
    Akira Takashima; Fiscal Year: 2005
    ..Preliminary results suggest that CD39 is responsible for ecto-ATPase and ADPase activities on LC and that LC-associated CD39 plays dual functions, i.e...
  31. A NOVEL APPROACH TO TARGET INFLAMMATION AND THROMBOSIS
    Harshal Nandurkar; Fiscal Year: 2007
    ..Inhibition of this interaction helps to maintain vascular patency. CD39/NTPase-1 is a key enzyme expressed on endothelial cells and platelets, which inhibits platelet reactivity and ..
  32. Joan H F Drosopoulos; Fiscal Year: 2014
    ..This unresponsiveness is due to EC CD39/ecto-ADPase, which rapidly metabolizes ADP released from activated platelets, thereby abolishing aggregation and ..
  33. THROMBOREGULATORY ROLE OF CD39 (ECTOADPASE) IN STROKE
    David Pinsky; Fiscal Year: 2004
    ..This observation culminated in our characterization of endothelial cell CD39/ecto-ADPase as the prime thromboregulator...
  34. DNA-Based Vaccines for Oral Carcnoma
    Theresa Whiteside; Fiscal Year: 2005
    ....
  35. Novel Approaches to the Assessment of Oral Mucosal Immu*
    Theresa Whiteside; Fiscal Year: 2003
    ..A better understanding of oral immunity in normal and HIV-infected mucosa is important for the development of new antiviral therapies, including oral antiviral vaccines. ..
  36. VASCULAR CONTROL OF BLOOD CELL REACTIVITY IN THROMBOSIS
    Aaron Marcus; Fiscal Year: 2005
    ..This observation culminated in our characterization of endothelial cell CD39/ecto-ADPase as the prime thromboregulator...
  37. Purinergic Thromboregulation
    Simon C Robson; Fiscal Year: 2010
    ..Cellular ectonucleotidases hydrolyze P2-mediators to down regulate P2 receptor-signaling responses. CD39 is the dominant vascular, T regulatory and Natural Killer (NK)T cell ectonucleotidase/NTPDase and plays a crucial ..
  38. FUNCTIONAL ANALYSIS OF VASCULAR NTPDASES
    Simon Robson; Fiscal Year: 2007
    ..Ectonucleotidases hydrolyze extra-cellular nucleotides, ultimately to the respective nucleosides. Members of the CD39 family of such ectoenzymes appear to be differentially expressed at high levels within the vasculature...
  39. VASCULAR NTPDASES AND TRANSPLANTATION
    Simon Robson; Fiscal Year: 2004
    ..g. by catalyzing the hydrolysis of ATP and/or ADP. We have shown CD39, a B-lymphocyte activation marker, to be the prototype member of an ectonucleotidase family, now termed nucleoside ..
  40. Regulation of EAE by skin immunization with self-peptide
    MARGARET BYNOE; Fiscal Year: 2008
    ..These studies will serve to advance our knowledge of autoimmune disease regulation both at the peripheral level and at the CNS level and could be beneficial to MS and other autoimmune diseases. ..
  41. Tolerance induction in EAE by epicutaneous immunization
    MARGARET BYNOE; Fiscal Year: 2006
    ..Finally, we will carry out experiments to test whether epicutaneous immunization with autoantigenic peptides is capable of ameliorating EAE. ..
  42. Mechanisms of Tumor Protection for T Cells by Hypoxia
    Michail Sitkovsky; Fiscal Year: 2009
    ....
  43. Tolerance to Carbohydrate Antigens in Transplantation
    David Cooper; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  44. Thromboregulation to Prevent Thrombotic Microangiopathy
    David Cooper; Fiscal Year: 2008
    ..It is anticipated that these approaches will abrogate the development of TM and allow long-term survival of pig organs in baboons. ..
  45. Hyperbaric Oxygenation May Increase Lung Injury
    Michail Sitkovsky; Fiscal Year: 2007
    ..unreadable]..
  46. CYCLOOXYGENASE-2 AND XENOBIOTICS IN VASCULAR INJURY
    Babette Weksler; Fiscal Year: 2005
    ..abstract_text> ..
  47. VASO-OCCLUSIVE PROCESSES IN SICKLE CELL RETINOPATHY
    Gerard Lutty; Fiscal Year: 2006
    ..abstract_text> ..
  48. ROLE OF ATP IN VASCULAR NEUROEFFECTOR PROCESS
    DAVID WESTFALL; Fiscal Year: 2006
    ..The third specific aim is to isolate and determine the protein structure of the soluble nucleotidases released during stimulation of sympathetic nerves. Standard protein isolation methods will be employed. ..
  49. Expression and Structure of Soluble Human Nucleotidases
    TERENCE KIRLEY; Fiscal Year: 2006
    ..Second, it will demonstrate how two different protein primary structures are used to do the same enzymatic task, aiding in the design of more efficacious therapeutic soluble nucleotidases. ..
  50. ACTIVE SITE DETERMINATION OF THE E-TYPE ATPASES
    TERENCE KIRLEY; Fiscal Year: 2004
    ..abstract_text> ..
  51. ADENOSINE DEAMINASE DEFICIENCY
    Linda Thompson; Fiscal Year: 2007
    ..Our data will contribute to the growing knowledge base needed to understand and control the vulnerability of the developing human immune system to environmental threats and infectious agents. ..
  52. Role of Epithelia In Ischemia Reperfusion Injury
    Sean P Colgan; Fiscal Year: 2010
    ..The overall aim of this proposal is to elucidate the transcriptional signaling events mediating mucosal epithelial responses to hypoxia and inflammation. ..