caspase-3

Summary

Gene Symbol: caspase-3
Description: caspase 3
Alias: CPP32, CPP32B, SCA-1, caspase-3, CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain, caspase 3, apoptosis-related cysteine peptidase, caspase 3, apoptosis-related cysteine protease, cysteine protease CPP32, procaspase3, protein Yama
Species: human

Top Publications

  1. ncbi Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3
    H Zou
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 90:405-13. 1997
  2. pmc Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity
    Dieter Demon
    Department for Molecular Biomedical Research, Flanders Institute for Biotechnology VIB, Ghent 9052, Belgium
    Mol Cell Proteomics 8:2700-14. 2009
  3. pmc Regulator of calcineurin 1 (RCAN1) facilitates neuronal apoptosis through caspase-3 activation
    Xiulian Sun
    Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 286:9049-62. 2011
  4. ncbi Genistein sensitizes TRAIL-resistant human gastric adenocarcinoma AGS cells through activation of caspase-3
    Cheng Yun Jin
    Department of Biological Sciences, Pusan National University, Busan 609 735, Republic of Korea
    Cancer Lett 257:56-64. 2007
  5. pmc Melatonin maintains mitochondrial membrane potential and attenuates activation of initiator (casp-9) and effector caspases (casp-3/casp-7) and PARP in UVR-exposed HaCaT keratinocytes
    T W Fischer
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Pineal Res 44:397-407. 2008
  6. pmc Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells
    Ying Ying Lu
    MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, PR China
    J Biomed Sci 16:16. 2009
  7. pmc Role of insulin-like growth factor binding protein 2 in lung adenocarcinoma: IGF-independent antiapoptotic effect via caspase-3
    Toshiro Migita
    The Cancer Institute, Tokyo, Japan
    Am J Pathol 176:1756-66. 2010
  8. pmc Caspase-3-dependent export of TCTP: a novel pathway for antiapoptotic intercellular communication
    I Sirois
    Research Centre, Centre hospitalier de l Université de Montréal CRCHUM, 1560 rue Sherbrooke Est, Montreal, Quebec, Canada
    Cell Death Differ 18:549-62. 2011
  9. pmc Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death
    S K Lai
    Division of Molecular and Cell Biology, School of Biological Sciences, College of Science, Nanyang Technological University, Singapore, Singapore
    Cell Death Differ 18:996-1004. 2011
  10. pmc Beclin 1 cleavage by caspase-3 inactivates autophagy and promotes apoptosis
    Yushan Zhu
    The Joint Laboratory of Apoptosis and Cancer Biology, College of Life Sciences, Nankai University, Tianjin 300071, China
    Protein Cell 1:468-77. 2010

Research Grants

  1. Regulation of caspase-1 by Sod2 in the heart
    Kumuda C Das; Fiscal Year: 2010
  2. Apoptosis Execution Pathways in Dopaminergic Cell Death
    Jun Chen; Fiscal Year: 2007
  3. Role of The Jak-Stat Pathway in Ischemic Preconditioning
    Yu Ting Xuan; Fiscal Year: 2010
  4. Mechanisms and Modulation of Cell Death in Traumatic Brain Injury
    ALAN IRA FADEN; Fiscal Year: 2012
  5. David Piwnica-Worms; Fiscal Year: 2016
  6. Dhyan Chandra; Fiscal Year: 2016
  7. Apoptosis and Trophoblast Fusion
    NEAL STEWART ROTE; Fiscal Year: 2010
  8. Mechanistic targets for intervention in sepsis-induced renal injury
    Philip R Mayeux; Fiscal Year: 2012
  9. Nucleotide Regulation of Apaf-1 Apoptosome and Apoptosis
    Dean G Tang; Fiscal Year: 2010
  10. The Role of Caspases in Warm and Cold Ischemia
    ALKESH HARIHAR JANI; Fiscal Year: 2010

Patents

  1. Therapeutic system targeting pathogen proteases and uses thereof

Detail Information

Publications297 found, 100 shown here

  1. ncbi Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3
    H Zou
    Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 90:405-13. 1997
    ..The COOH-terminal region of Apaf-1 comprises multiple WD repeats, which are proposed to mediate protein-protein interactions. Cytochrome c binds to Apaf-1, an event that may trigger the activation of caspase-3, leading to apoptosis...
  2. pmc Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity
    Dieter Demon
    Department for Molecular Biomedical Research, Flanders Institute for Biotechnology VIB, Ghent 9052, Belgium
    Mol Cell Proteomics 8:2700-14. 2009
    ..Interestingly, specific cleavage by caspase-7 relies on excluding recognition by caspase-3 and not on increasing binding for caspase-7...
  3. pmc Regulator of calcineurin 1 (RCAN1) facilitates neuronal apoptosis through caspase-3 activation
    Xiulian Sun
    Townsend Family Laboratories, Department of Psychiatry, Brain Research Center, Graduate Program in Neuroscience, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 286:9049-62. 2011
    ....
  4. ncbi Genistein sensitizes TRAIL-resistant human gastric adenocarcinoma AGS cells through activation of caspase-3
    Cheng Yun Jin
    Department of Biological Sciences, Pusan National University, Busan 609 735, Republic of Korea
    Cancer Lett 257:56-64. 2007
    ..These results indicate that caspase-3 is a key regulator of apoptosis in response to combined genistein and TRAIL in human gastric adenocarcinoma AGS cells through the activation of DR5 and mitochondrial dysfunction...
  5. pmc Melatonin maintains mitochondrial membrane potential and attenuates activation of initiator (casp-9) and effector caspases (casp-3/casp-7) and PARP in UVR-exposed HaCaT keratinocytes
    T W Fischer
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Pineal Res 44:397-407. 2008
    ..In conclusion, these data provide detailed evidence for specific antiapoptotic mechanisms of melatonin in UVR-induced damage of human keratinocytes...
  6. pmc Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells
    Ying Ying Lu
    MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, PR China
    J Biomed Sci 16:16. 2009
    ..DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although the molecular mechanisms are not well understood...
  7. pmc Role of insulin-like growth factor binding protein 2 in lung adenocarcinoma: IGF-independent antiapoptotic effect via caspase-3
    Toshiro Migita
    The Cancer Institute, Tokyo, Japan
    Am J Pathol 176:1756-66. 2010
    ..IGFBP-2, therefore, may offer a novel therapeutic target and serve as an antiapoptotic biomarker for lung adenocarcinoma...
  8. pmc Caspase-3-dependent export of TCTP: a novel pathway for antiapoptotic intercellular communication
    I Sirois
    Research Centre, Centre hospitalier de l Université de Montréal CRCHUM, 1560 rue Sherbrooke Est, Montreal, Quebec, Canada
    Cell Death Differ 18:549-62. 2011
    ..Collectively, these results identify TCTP-bearing nanovesicles as a novel component of the paracrine apoptotic program of potential importance in vascular repair...
  9. pmc Caspase-3-mediated degradation of condensin Cap-H regulates mitotic cell death
    S K Lai
    Division of Molecular and Cell Biology, School of Biological Sciences, College of Science, Nanyang Technological University, Singapore, Singapore
    Cell Death Differ 18:996-1004. 2011
    ..Taken together, we provide new insights into the molecular events that occur during mitotic death...
  10. pmc Beclin 1 cleavage by caspase-3 inactivates autophagy and promotes apoptosis
    Yushan Zhu
    The Joint Laboratory of Apoptosis and Cancer Biology, College of Life Sciences, Nankai University, Tianjin 300071, China
    Protein Cell 1:468-77. 2010
    ..Thus, our results suggest that the cleavage of Beclin 1 by caspase-3 may contribute to inactivate autophagy leading towards augmented apoptosis...
  11. doi Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3
    Hai Qiang Wang
    Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, 15 Changle Western Road, Xi an, People s Republic of China
    J Pathol 225:232-42. 2011
    ..Our results suggest that deregulated miR-155 promotes Fas-mediated apoptosis in human IDD by targeting FADD and caspase-3, implicating an aetiological and therapeutic role of miR-155 in IDD...
  12. pmc Bcl2L12-mediated inhibition of effector caspase-3 and caspase-7 via distinct mechanisms in glioblastoma
    Alexander H Stegh
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:10703-8. 2008
    ..Thus, alphaB-crystallin is a Bcl2L12-induced oncoprotein that enables Bcl2L12 to block the activation of both effector caspases via distinct mechanisms, thereby contributing to GBM pathogenesis and its hallmark biological properties...
  13. doi Transglutaminase 2 suppresses apoptosis by modulating caspase 3 and NF-kappaB activity in hypoxic tumor cells
    G Y Jang
    Department of Biochemistry and Molecular Biology Aging and Apoptosis Research Center AARC, Seoul National University College of Medicine, Seoul 110 799, Korea
    Oncogene 29:356-67. 2010
    ..These findings suggest that the inhibition of TG2 may offer a novel strategy for anticancer therapy...
  14. ncbi Phosphorylation-regulated cleavage of the tumor suppressor PTEN by caspase-3: implications for the control of protein stability and PTEN-protein interactions
    Josema Torres
    Instituto de Investigaciones Citologicas, Amadeo de Saboya 4, Valencia 46010, Spain
    J Biol Chem 278:30652-60. 2003
    ..Our findings suggest the existence of a regulatory mechanism of protein stability and PTEN-protein interactions during apoptosis, executed by caspase-3 in a PTEN phosphorylation-regulated manner...
  15. ncbi Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3
    G Paroni
    Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Biologia, Universita di Udine, P le Kolbe 4, Udine 33100, Italy
    J Biol Chem 276:21907-15. 2001
    ..Our findings suggest a role played by caspase-2 as a regulator of the mitochondrial integrity and open questions on the mechanisms responsible for its activation during cell death...
  16. ncbi Polypyrimidine tract-binding proteins are cleaved by caspase-3 during apoptosis
    Sung Hoon Back
    National Research Laboratory, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Hyoja Dong, Pohang, Kyungbuk 790 784, Korea
    J Biol Chem 277:27200-9. 2002
    ..This suggests that translation of some IRES-containing mRNAs is regulated by proteolytic cleavage of PTB during apoptosis...
  17. ncbi Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis
    Chi Ying F Huang
    Department of Biological Science and Technology, National Chiao Tung University, 75 Po Ai Street, Hsinchu 300, Taiwan, Republic of China
    J Biol Chem 277:34367-74. 2002
    ..Collectively, these results support the notion that caspase-mediated proteolytic activation of Mst3 contributes to apoptosis...
  18. ncbi Presentation of nitric oxide regulates monocyte survival through effects on caspase-9 and caspase-3 activation
    Mandy M Zeigler
    Dorothy M Davis Heart and Lung Research Institute, The Department of Internal Medicine, Ohio State University, Columbus, Ohio 43210 1252, USA
    J Biol Chem 278:12894-902. 2003
    ..These data support the hypothesis that nitrosylation of protein thiol residues by nitric oxide is critical for promoting the survival of human monocytes...
  19. ncbi Nitric oxide protects human extravillous trophoblast cells from apoptosis by a cyclic GMP-dependent mechanism and independently of caspase 3 nitrosylation
    Philip R Dash
    Department of Biochemistry and Immunology, St George s Hospital Medical School, University of London, Cranmer Terrace, London SW17 ORE, UK
    Exp Cell Res 287:314-24. 2003
    ..induced by a combination of tumour necrosis factor alpha and actinomycin D and to suppress the activity of caspase 3. Treatment with hepatocyte growth factor (HGF) stimulated expression of the inducible isoform of NO synthase and ..
  20. ncbi Caspase 3-mediated proteolysis of the N-terminal cytoplasmic domain of the human erythroid anion exchanger 1 (band 3)
    Debabrata Mandal
    Department of Chemistry, Bose Institute, 93 1 Acharya, Prafalla Chandra Road, Kolkata 700009, India
    J Biol Chem 278:52551-8. 2003
    ..demonstrated that the N-terminal cytoplasmic domain of band 3 (CDB3) is a substrate of the apoptosis executioner caspase 3 (1). CDB3 has two non-conventional caspase 3 cleavage sites, TATD45 and EQGD205 (2)...
  21. ncbi STAT1-induced apoptosis is mediated by caspases 2, 3, and 7
    Juan J Sironi
    Derald H Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York University, New York, New York 10029, USA
    J Biol Chem 279:4066-74. 2004
    ..These results suggest that prolonged nuclear localization of activated STAT1 results in apoptosis involving specific regulation of caspase pathway...
  22. ncbi Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3
    Yu Hauh Wu
    Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei 10063, Taiwan, Republic of China
    J Biol Chem 279:19264-75. 2004
    ..Together, our results suggest a model that ricin triggers morphological changes of apoptosis by caspase-3-mediated proteolytic activation of BAT3...
  23. ncbi Caspase-3 mediated feedback activation of apical caspases in doxorubicin and TNF-alpha induced apoptosis
    Shihe Yang
    Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Apoptosis 11:1987-97. 2006
    ..The significant impact of caspase-3 on both effector and apical caspases suggests that modulation of caspase-3 activity would be a useful approach to overcome drug resistance in clinical oncology...
  24. ncbi Reversible glutathiolation of caspase-3 by glutaredoxin as a novel redox signaling mechanism in tumor necrosis factor-alpha-induced cell death
    Michelle C Sykes
    Circ Res 100:152-4. 2007
  25. ncbi The most widespread desmosomal cadherin, desmoglein 2, is a novel target of caspase 3-mediated apoptotic machinery
    Nicola Cirillo
    Center on Craniofacial Malformations MRI, Regione Campania, 1st School of Medicine and Surgery, II University of Naples, 80138 Naples, Italy
    J Cell Biochem 103:598-606. 2008
    ..All these biochemical, morphological, and functional changes were regulated by caspase 3. Indeed, in the presence of the caspase 3-inhibitor z-DEVD-fmk, full-length Dsg2 protein levels were preserved, ..
  26. ncbi Cytosolic antiviral RNA recognition pathway activates caspases 1 and 3
    Johanna Rintahaka
    Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Topeliuksenkatu 41a, Helsinki, Finland
    J Immunol 180:1749-57. 2008
    ..Furthermore, caspase 3-dependent processing of pro-IL-18 was also observed in human HaCaT keratinocytes, and forced expression of RIG-I ..
  27. doi Caspase-3 activation triggers extracellular cathepsin L release and endorepellin proteolysis
    Jean Francois Cailhier
    Research Centre, Centre Hospitalier Universitaireé de Montréal and Montreal Cancer Institute Université de Montréal, Montreal, Quebec H2L 4M1, Canada
    J Biol Chem 283:27220-9. 2008
    ..These data demonstrate that caspase-3 activation is a novel pathway of importance for triggering extracellular CathL release and the cleavage of extracellular matrix components...
  28. doi Up-regulation of micro-RNA-221 (miRNA-221; chr Xp11.3) and caspase-3 accompanies down-regulation of the survivin-1 homolog BIRC1 (NAIP) in glioblastoma multiforme (GBM)
    W J Lukiw
    LSU Neuroscience Center of Excellence and LSU Department of Neurosurgery, Louisiana State University Health Sciences Center, 2020 Gravier Street, Suite 8A4, New Orleans, LA, 70112, USA
    J Neurooncol 91:27-32. 2009
    ....
  29. doi Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide
    Toshikazu Shioiri
    Research Institute of Pharmaceutical Sciences, Musashino University, 1 1 20 Shinmachi, Nishitokyo shi, Tokyo 202 8585, Japan
    Biochim Biophys Acta 1792:1011-8. 2009
    ..Failure of this escape mechanism may result in endothelial injury/dysfunction...
  30. pmc Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity
    XIAONAN H WANG
    Renal Division, Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 285:21249-57. 2010
    ..Indeed, we found that in mice with a muscle wasting condition, chronic kidney disease, there was cleavage of subunits Rpt2 and Rpt6 and stimulation of proteasome activity...
  31. pmc Disassembly of the JIP1/JNK molecular scaffold by caspase-3-mediated cleavage of JIP1 during apoptosis
    Mahesh Vaishnav
    Department of Biochemistry, Henry Wellcome Building, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK
    Exp Cell Res 317:1028-39. 2011
    ..Caspase-mediated cleavage of JIP scaffold proteins may therefore represent an important mechanism for modulation of JNK signalling during apoptotic cell death...
  32. pmc Low doses of the novel caspase-inhibitor GS-9450 leads to lower caspase-3 and -8 expression on peripheral CD4+ and CD8+ T-cells
    J E Arends
    Department of Internal Medicine and Infectious Diseases, University Medical Center Utrecht UMCU, Heidelberglaan, GA, Utrecht, The Netherlands
    Apoptosis 16:959-66. 2011
    ..Furthermore, at baseline percentages of activated caspase-3, activated caspase-8 and CD95+ T-cells were higher in chronic HCV-infected patients compared to healthy controls...
  33. pmc Prostate apoptosis response 4 (Par-4), a novel substrate of caspase-3 during apoptosis activation
    Parvesh Chaudhry
    Research Group in Molecular Oncology and Endocrinology, Department of Chemistry Biology, Universite du Quebec a Trois Rivieres, Trois Rivieres, Quebec, Canada
    Mol Cell Biol 32:826-39. 2012
    ..We have identified a novel specific caspase-3 cleavage site in Par-4, and the cleaved fragment of Par-4 retains proapoptotic activity...
  34. pmc ALS-associated ataxin 2 polyQ expansions enhance stress-induced caspase 3 activation and increase TDP-43 pathological modifications
    Michael P Hart
    Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
    J Neurosci 32:9133-42. 2012
    ..intermediate-length ataxin 2 polyQ expansions to the stress-dependent activation of multiple caspases, including caspase 3. Caspase activation is upstream of TDP-43 cleavage and phosphorylation since caspase inhibitors block these ..
  35. pmc Proteolytic activation of protein kinase C delta by an ICE/CED 3-like protease induces characteristics of apoptosis
    T Ghayur
    BASF Bioresearch Corporation, Worcester, Massachusetts 01605, USA
    J Exp Med 184:2399-404. 1996
    ..demonstrate that full-length PKC delta is cleaved at DMQD330N to a catalytically active fragment by the cysteine protease CPP32. The results also demonstrate that overexpression of the catalytic kinase fragment in cells is associated ..
  36. ncbi Caspase-3-mediated processing of poly(ADP-ribose) glycohydrolase during apoptosis
    E B Affar
    Health and Environment Unit, Laval University Medical Research Center, Centre Hospitalier Universitaire de Quebec, Canada
    J Biol Chem 276:2935-42. 2001
    ..The early cleavage of both PARP-1 and PARG by caspases during apoptosis suggests an important function for poly(ADP-ribose) metabolism regulation during this cell death process...
  37. ncbi Central role of glycogen synthase kinase-3beta in endoplasmic reticulum stress-induced caspase-3 activation
    Ling Song
    Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0017, USA
    J Biol Chem 277:44701-8. 2002
    ..Thus, ER stress activates GSK3beta through dephosphorylation of phospho-Ser-9, a prerequisite for caspase-3 activation, and this process is amenable to pharmacological intervention...
  38. ncbi Identification of ASK1, MKK4, JNK, c-Jun, and caspase-3 as a signaling cascade involved in cadmium-induced neuronal cell apoptosis
    Sun Don Kim
    Department of Biomedical Sciences, National Institute of Health, Seoul 122 701, Republic of Korea
    Biochem Biophys Res Commun 328:326-34. 2005
    ..Taken together, our data suggested that the JNK/c-Jun signaling cascade plays a crucial role in Cd-induced neuronal cell apoptosis and provides a molecular linkage between oxidative stress and neuronal apoptosis...
  39. pmc TRAIL, DR5 and caspase 3-dependent apoptosis in vessels of diseased human temporomandibular joint disc. An immunohistochemical study
    C Loreto
    Department of Anatomy, Diagnostic Pathology, Forensic Medicine, Hygiene and Public Health, University of Catania, Via S Sofia 87, Catania, Italy
    Eur J Histochem 54:e40. 2010
    ..disc vessels, we assessed, by immunohistochemistry, the detection of TRAIL, its death receptor DR5 and caspase 3. TRAIL, its death receptor DR5 and caspase 3 expression were studied by immunohistochemistry in 15 TMJ discs ..
  40. ncbi The novel tyrosine kinase inhibitor EXEL-0862 induces apoptosis in human FIP1L1-PDGFR-alpha-expressing cells through caspase-3-mediated cleavage of Mcl-1
    J Pan
    Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Leukemia 21:1395-404. 2007
    ..Our data establish EXEL-0862 as a solid candidate for the targeted treatment of patients with FIP1L1-PDGFR-alpha-positive HES...
  41. doi Tau cleavage at D421 by caspase-3 is induced in neurons and astrocytes infected with herpes simplex virus type 1
    Rodrigo Lerchundi
    Institute of Clinical Microbiology, Faculty of Medicine, University Austral of Chile, Valdivia, Chile
    J Alzheimers Dis 23:513-20. 2011
    ..In conclusion, our findings support the idea that HSV-1 could contribute to induce neurodegenerative processes in age-associated pathologies such as Alzheimer's disease...
  42. ncbi Involvement of caspase-4(-like) protease in Fas-mediated apoptotic pathway
    S Kamada
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Suita, Japan
    Oncogene 15:285-90. 1997
    Proteases of the caspase family, especially caspase-1 (ICE)(-like), caspase-3 (CPP32/Yama/apopain)(-like) and caspase-8 (MACH/FLICE/Mch5) proteases, are implicated in Fas (APO-1/CD95)-mediated apoptosis...
  43. pmc p53 inhibits alpha 6 beta 4 integrin survival signaling by promoting the caspase 3-dependent cleavage of AKT/PKB
    R E Bachelder
    Division of Cancer Biology and Angiogenesis, Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Cell Biol 147:1063-72. 1999
    ..The involvement of caspase 3 in AKT/PKB regulation was indicated by the ability of Z-DEVD-FMK, a caspase 3 inhibitor, to block the ..
  44. pmc Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells
    Maria Ruzzene
    Dipartimento di Chimica Biologica, Universita di Padova, Viale G Colombo, 3 35121 Padova, Italy
    Biochem J 364:41-7. 2002
    ....
  45. ncbi Interleukin-4 inhibits caspase-3 by regulating several proteins in the Fas pathway during initial stages of human T helper 2 cell differentiation
    Kirsi J Rautajoki
    Turku Centre for Biotechnology, University of Turku and Abo Akademi, Tykistokatu 6A, 5th Floor, FIN 20521 Turku, Finland
    Mol Cell Proteomics 6:238-51. 2007
    ..Taken together, our results indicate that IL-4 inhibits caspase activity during the initial stages of human Th2 cell differentiation by regulating expression of several key players in the Fas-induced pathway...
  46. doi Mutational analysis of CASP1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 14 genes in gastrointestinal stromal tumors
    Yoo Ri Kim
    Department of Pathology, The Catholic University of Korea, Seoul, Korea
    Hum Pathol 40:868-71. 2009
    ....
  47. pmc Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells
    B B Zhou
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 95:6785-90. 1998
    ..Our results do not support the idea that apoptosis is simply an aberrant mitosis but, instead, suggest that a subset of mitotic mechanisms plays an important role in apoptosis through elevated cdk activities...
  48. ncbi Caspase-3 is required for alpha-fodrin cleavage but dispensable for cleavage of other death substrates in apoptosis
    R U Jänicke
    Institute of Molecular and Cell Biology, The National University of Singapore, 30 Medical Dr, Singapore 117609, Republic of Singapore
    J Biol Chem 273:15540-5. 1998
    ....
  49. ncbi HIV-1 protein Tat induces apoptosis of hippocampal neurons by a mechanism involving caspase activation, calcium overload, and oxidative stress
    I I Kruman
    Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky, 40536, USA
    Exp Neurol 154:276-88. 1998
    ..Agents that interupt this apoptotic cascade may prove beneficial in preventing neuronal degeneration and associated dementia in AIDS patients...
  50. ncbi Caspase-dependent apoptosis of cells expressing the chemokine receptor CXCR4 is induced by cell membrane-associated human immunodeficiency virus type 1 envelope glycoprotein (gp120)
    M Biard-Piechaczyk
    Laboratoire Infections Retrovirales et Signalisation Cellulaire, CNRS EP 2104, Institut de Biologie, 4 Boulevard Henri IV, Montpellier Cedex, 34060, France
    Virology 268:329-44. 2000
    ..fmk) and a relatively selective inhibitor of caspase 3 (z-DEVD.fmk)...
  51. ncbi Caspase cleavage of vimentin disrupts intermediate filaments and promotes apoptosis
    Y Byun
    Center for Endocrinology, Metabolism and Molecular Medicine, Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA
    Cell Death Differ 8:443-50. 2001
    ..Taken together, our findings suggest that caspase proteolysis of vimentin promotes apoptosis by dismantling intermediate filaments and by amplifying the cell death signal via a pro-apoptotic cleavage product...
  52. ncbi The fate of desmosomal proteins in apoptotic cells
    J Weiske
    Institute of Clinical Chemistry and Pathobiochemistry, University Hospital Benjamin Franklin, Hindenburgdamm 30, 12200 Berlin, Germany
    J Biol Chem 276:41175-81. 2001
    ..Immunofluorescence analysis confirmed that this cleavage results in the disruption of the desmosome structure and thus contributes to cell rounding and disintegration of the intermediate filament system...
  53. ncbi Role of caspases in Ox-LDL-induced apoptotic cascade in human coronary artery endothelial cells
    Jiawei Chen
    Department of Internal Medicine, University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA
    Circ Res 94:370-6. 2004
    ..This is followed by activation of apoptotic signaling pathway, involving release of cytochrome c and Smac and activation of caspase-9 and then caspase-3...
  54. ncbi Caspase-3 has a nonapoptotic function in erythroid maturation
    Graeme W Carlile
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, Box 112, 1275 York Ave, New York, NY 10021, USA
    Blood 103:4310-6. 2004
    ..These results demonstrate that caspase-3 is required for efficient erythropoiesis in this model system...
  55. ncbi Suppressive effect of elongation factor 2 on apoptosis induced by HIV-1 viral protein R
    S Zelivianski
    Children s Memorial Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL 60614, USA
    Apoptosis 11:377-88. 2006
    ..The anti-apoptotic property of EF2 is demonstrated by its ability to suppress caspase 9 and caspase 3-mediated apoptosis induced by Vpr...
  56. ncbi Early caspase-3 activation independent of apoptosis is required for cellular function
    Juan A Rosado
    Department of Physiology Cellular Physiology Research Group, University of Extremadura, Caceres, Spain
    J Cell Physiol 209:142-52. 2006
    ..Thus, early activation of caspase-3 seems to be a non-apoptotic event required for cellular function...
  57. ncbi CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme
    T Fernandes-Alnemri
    Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107
    J Biol Chem 269:30761-4. 1994
    ..The new gene encodes a 32-kDa putative cysteine protease (CPP32) with significant homology to Caenorhabditis elegans cell death protein Ced-3, mammalian interleukin-1 beta-..
  58. pmc The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases
    N Roy
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 16:6914-25. 1997
    ..Taken together, these findings suggest that c-IAP-1 and c-IAP-2 function similarly to XIAP by inhibiting the distal cell death proteases, caspases-3 and -7, whereas NAIP presumably inhibits apoptosis via other targets...
  59. ncbi Apoptotic cell death upon contact of CD4+ T lymphocytes with HIV glycoprotein-expressing cells is mediated by caspases but bypasses CD95 (Fas/Apo-1) and TNF receptor 1
    H Ohnimus
    Institute for Virology and Immunobiology, Julius Maximilians University, Wurzburg, Germany
    J Immunol 159:5246-52. 1997
    ..Elucidation of the mechanism that initiates apoptosis in this situation will add to our understanding of both HIV pathogenesis and apoptotic signaling...
  60. ncbi Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing
    M Sebbagh
    INSERM U461, Faculte de Pharmacie, 92296 Chatenay Malabry, France
    Nat Cell Biol 3:346-52. 2001
    ..We also show that expression of active truncated ROCK I induces cell blebbing. Thus, activation of ROCK I by caspase-3 seems to be responsible for bleb formation in apoptotic cells...
  61. ncbi Down-regulation of caspase 3 in breast cancer: a possible mechanism for chemoresistance
    Eswaran Devarajan
    Department of Bioimmunotherapy, The University of Texas MD Anderson Cancer Center, Houston, Texas, TX 77030, USA
    Oncogene 21:8843-51. 2002
    ..These results suggest that the loss of caspases-3 expression may represent an important cell survival mechanism in breast cancer patients...
  62. ncbi Caspase 3 in breast cancer
    Norma O'Donovan
    Department of Medical Oncology, St Vincent s University Hospital, University College Dublin, Dublin 4, Ireland
    Clin Cancer Res 9:738-42. 2003
    ..One of the key mediators of apoptosis is the thiol protease caspase 3. In this investigation, caspase 3 mRNA and protein expression in breast cancer was examined.
  63. pmc Vpr R77Q is associated with long-term nonprogressive HIV infection and impaired induction of apoptosis
    Julian J Lum
    Ottawa Health Research Institute, University of Ottawa, Ottawa, Ontario, Canada
    J Clin Invest 111:1547-54. 2003
    ..These data highlight the pathophysiologic role of Vpr in HIV-induced immune disease and suggest a novel mechanism of LTNP...
  64. ncbi Mitochondrion-dependent caspase activation by the HIV-1 envelope
    Thomas Roumier
    Centre National de la Recherche Scientifique, UMR 8125, Institut Gustave Roussy, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Biochem Pharmacol 66:1321-9. 2003
    ..Conversely, inhibition of caspases did not prevent MMP. These results indicate that Env-elicited syncytial apoptosis involves the intrinsic (mitochondrial) pathway...
  65. ncbi HIV gp41-induced apoptosis is mediated by caspase-3-dependent mitochondrial depolarization, which is inhibited by HIV protease inhibitor nelfinavir
    Himanshu Garg
    NCI Frederick, P O Box B, Building 469, Room 152, Miller Drive, Frederick, MD 21702 1201, USA
    J Leukoc Biol 79:351-62. 2006
    ..We also show that virion-induced apoptosis is gp41-dependent. Our findings provide new insights into the mechanism via which HIV gp41 mediates apoptosis in bystander cells...
  66. ncbi Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1
    Jean Antoine Ribeil
    CNRS UMR 8147, Faculté de Médecine et Université René Descartes Paris V, Institut Fédérative Necker, 75270 Paris, France
    Nature 445:102-5. 2007
    ..These effects are abrogated by the transduction of a caspase-resistant GATA-1 mutant. Thus, in erythroid precursors undergoing terminal differentiation, Hsp70 prevents active caspase-3 from cleaving GATA-1 and inducing apoptosis...
  67. pmc SAG/ROC-SCF beta-TrCP E3 ubiquitin ligase promotes pro-caspase-3 degradation as a mechanism of apoptosis protection
    Mingjia Tan
    Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0936, USA
    Neoplasia 8:1042-54. 2006
    ..Thus, pro-caspase-3 appears to be a substrate of SAG/ROC-SCF(beta-TrCP) E3 Ub ligase, which protects cells from apoptosis through increased apoptosis threshold by reducing the basal level of pro-caspase-3...
  68. pmc The caspase-8 dimerization/dissociation balance is a highly potent regulator of caspase-8, -3, -6 signaling
    Maximilian L Würstle
    Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin 2, Ireland
    J Biol Chem 285:33209-18. 2010
    ..Our study therefore highlights a central and so far underappreciated role of caspase-8 dimerization/dissociation in avoiding unwanted cell death by lethal amplification of caspase responses via the caspase-8, -3, -6 loop...
  69. ncbi Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
    D W Nicholson
    Department of Biochemistry and Molecular Biology, Merck Frosst Centre for Therapeutic Research, Pointe Claire Dorval, Quebec, Canada
    Nature 376:37-43. 1995
    ..This enzyme, named apopain, is composed of two subunits of relative molecular mass (M(r)) 17K and 12K that are derived from a common ..
  70. ncbi Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase
    M Tewari
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA
    Cell 81:801-9. 1995
    ..We cloned a ced-3/ICE-related gene, designated Yama, that encodes a protein identical to CPP32 beta. Purified Yama was a zymogen that, when activated, cleaved PARP to generate the 85 kDa apoptotic fragment...
  71. ncbi Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract
    Y P Goldberg
    Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada
    Nat Genet 13:442-9. 1996
    Apoptosis has recently been recognized as a mode of cell death in Huntington disease (HD). Apopain, a human counterpart of the nematode cysteine protease death-gene product, CED-3, has a key role in proteolytic events leading to ..
  72. pmc Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases
    S M Srinivasula
    Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 93:14486-91. 1996
    ..protease cascade that leads to activation of ICE-like proteases (TX, ICE, and ICE-relIII), Ced-3-like proteases (CPP32, Mch2, Mch3, Mch4, and Mch6), and the ICH-1 protease...
  73. ncbi Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis
    S Kothakota
    Chiron Corporation, Emeryville, CA 94608, USA
    Science 278:294-8. 1997
    The caspase-3 (CPP32, apopain, YAMA) family of cysteinyl proteases has been implicated as key mediators of apoptosis in mammalian cells...
  74. pmc Caspases: the executioners of apoptosis
    G M Cohen
    MRC Toxicology Unit, University of Leicester, U K
    Biochem J 326:1-16. 1997
    ..Cells undergoing apoptosis following triggering of death receptors execute the death programme by activating a hierarchy of caspases, with caspase-8 and possibly caspase-10 being at or near the apex of this apoptotic cascade...
  75. pmc Dismantling cell-cell contacts during apoptosis is coupled to a caspase-dependent proteolytic cleavage of beta-catenin
    C Brancolini
    Laboratorio Nazionale Consorzio Interuniversitario Biotecnologie AREA Science Park, 34142 Trieste, Italy
    J Cell Biol 139:759-71. 1997
    ..Caspase-3 (cpp32/apopain/yama) cleaves in vitro translated beta-catenin into a form which is similar in size to that observed in cells ..
  76. ncbi A single BIR domain of XIAP sufficient for inhibiting caspases
    R Takahashi
    Burnham Institute, Program on Apoptosis and Cell Death Regulation, La Jolla, California 92037, USA
    J Biol Chem 273:7787-90. 1998
    ..These findings identify BIR2 as the minimal caspase-inhibitory domain of XIAP and indicate that a single BIR domain can be sufficient for binding and inhibiting caspases...
  77. ncbi Nitric oxide inhibits caspase-3 by S-nitrosation in vivo
    L Rossig
    Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    J Biol Chem 274:6823-6. 1999
    ..Thus, NO supplied by exogenous NO donors serves in vivo as an antiapoptotic regulator of caspase activity via S-nitrosation of the Cys-163 residue of caspase-3...
  78. pmc Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis
    M Mancini
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 149:603-12. 2000
    ..We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors...
  79. ncbi Regulation of apoptosis by phosphatidylinositol 4,5-bisphosphate inhibition of caspases, and caspase inactivation of phosphatidylinositol phosphate 5-kinases
    M Mejillano
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 276:1865-72. 2001
    ..4,5-bisphosphate (PIP2) is a direct inhibitor of initiator caspases 8 and 9, and their common effector caspase 3. PIP2 inhibited procaspase 9 processing in cell extracts and in a reconstituted procaspase 9/Apaf1 apoptosome ..
  80. ncbi Structural basis of caspase-7 inhibition by XIAP
    J Chai
    Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, NJ 08544, USA
    Cell 104:769-80. 2001
    ..Our biochemical and structural analyses reveal that the BIR domains are dispensable for the inhibition of caspase-3 and -7. This study provides a structural basis for the design of the next-generation caspase inhibitors...
  81. ncbi Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death
    S Kagawa
    Department of Thoracic and Cardiovascular Surgery, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Clin Cancer Res 7:1474-80. 2001
    ..Together, these results suggest that a deficit in caspase-3 is not sufficient to block Bax-induced cell death...
  82. ncbi D4-GDI is cleaved by caspase-3 during daunorubicin-induced apoptosis in HL-60 cells
    Kang Beom Kwon
    Department of Physiology, School of Oriental Medicine, Won Kwang University, Chonbuk, Korea
    Exp Mol Med 34:32-7. 2002
    ..drug, is known to induce apoptosis in HL-60 cells in a dose-dependent manner through the activation of caspase-3 (CPP32)...
  83. ncbi HIV-1 Vpr induces apoptosis through caspase 9 in T cells and peripheral blood mononuclear cells
    Karuppiah Muthumani
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:37820-31. 2002
    ..Activation of the caspase 9 pathway resulted in caspase 3 activation and apoptosis in human primary cells...
  84. pmc The mitochondriotoxic domain of Vpr determines HIV-1 virulence
    Catherine Brenner
    Centre National de la Recherche Scientifique, Formation de Recherche en Evolution 2445, Université de Versailles St Quentin, Versailles, France
    J Clin Invest 111:1455-7. 2003
  85. pmc NF-kappaB and p53 are the dominant apoptosis-inducing transcription factors elicited by the HIV-1 envelope
    Jean Luc Perfettini
    Centre National de la Recherche Scientifique, UMR 8125, Institut Gustave Roussy, F 94805 Villejuif, France
    J Exp Med 199:629-40. 2004
    ..Altogether, these data indicate that NF-kappaB and p53 cooperate as the dominant proapoptotic transcription factors participating in HIV-1 infection...
  86. ncbi Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase
    Till Bartke
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 14:801-11. 2004
    ..Our work suggests that, owing to its two activities and its localization, BRUCE may function as a specialized regulator of cell death pathways...
  87. ncbi Nuclear translocation of caspase-3 is dependent on its proteolytic activation and recognition of a substrate-like protein(s)
    Shinji Kamada
    Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA
    J Biol Chem 280:857-60. 2005
    ..These results suggest that active caspase-3 is translocated in association with a substrate-like protein(s) from the cytoplasm into the nucleus during progression through apoptosis...
  88. pmc Human Immunodeficiency virus type 1 Nef potently induces apoptosis in primary human brain microvascular endothelial cells via the activation of caspases
    Edward A Acheampong
    Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Virol 79:4257-69. 2005
    ..The results of this study demonstrate that Nef likely contributes to the neuroinvasion and neuropathogenesis of HIV-1, through its effects on select cellular processes, including various apoptotic cascades...
  89. ncbi The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes
    Rachel L Dusek
    Department of Pathology and Dermatology and Cell Death Regulation Laboratory, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Biol Chem 281:3614-24. 2006
    ....
  90. ncbi Proteolytic regulation of nuclear factor of activated T (NFAT) c2 cells and NFAT activity by caspase-3
    Wenfang Wu
    Immunobiology Program, Department of Medicine, The University of Vermont College of Medicine, Burlington, Vermont 05405, USA
    J Biol Chem 281:10682-90. 2006
    ..Our findings reveal a new post-transcriptional regulation of NFATc2 that operates, not only during apoptosis, but also in non-apoptotic effector T cells...
  91. ncbi Caspase-3-dependent activation of calcium-independent phospholipase A2 enhances cell migration in non-apoptotic ovarian cancer cells
    Xiaoxian Zhao
    Department of Cancer Biology, The Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 281:29357-68. 2006
    ..Importantly, small interfering RNA-mediated down-regulation of endogenous iPLA(2) expression in ovarian carcinoma HEY cells results in decreased migration toward laminin, suggesting that our findings are pathophysiologically important...
  92. pmc Human and murine granzyme B exhibit divergent substrate preferences
    Sean P Cullen
    Molecular Cell Biology Laboratory, Department of Genetics, The Smurfit Institute, Trinity College, Dublin 2, Ireland
    J Cell Biol 176:435-44. 2007
    ..These data demonstrate that human and murine GzmB are distinct enzymes with different substrate preferences. Our observations also illustrate how subtle differences in enzyme structure can radically affect substrate selection...
  93. pmc Platelet-derived growth factor protects neurons against gp120-mediated toxicity
    Fuwang Peng
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Neurovirol 14:62-72. 2008
    ..Taken together these findings lead us to suggest that PDGF-BB could be considered as a therapeutic agent that can mitigate gp120-mediated neurotoxicity in HAD...
  94. pmc Virion-associated Vpr of human immunodeficiency virus type 1 triggers activation of apoptotic events and enhances fas-induced apoptosis in human T cells
    Hubert Arokium
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, David Geffen School of Medicine, 615 Charles E Young Dr South, BSRB 173, Los Angeles, CA 90095, USA
    J Virol 83:11283-97. 2009
    ..These findings suggest that virion-associated Vpr can contribute to the depletion of CD4(+) lymphocytes either directly or by enhancing Fas-mediated apoptosis during acute HIV-1 infection and in AIDS...
  95. doi Caspase-3 cleaves XIAP in a positive feedback loop to sensitize melanoma cells to TRAIL-induced apoptosis
    M Hörnle
    Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, Baden Wurttemberg, Germany
    Oncogene 30:575-87. 2011
    ..The molecular mechanism discovered here appears to have broader implications, because cleavage of XIAP was also shown to accompany cisplatin-induced sensitization of melanoma cells to TRAIL...
  96. pmc Cleavage of sterol regulatory element binding proteins (SREBPs) by CPP32 during apoptosis
    X Wang
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA
    EMBO J 15:1012-20. 1996
    ..b>CPP32, a cysteine protease, was shown previously to cleave SREBP-1 and SREBP-2 in vitro at an aspartic acid between the ..
  97. ncbi Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
    X Liu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cell 86:147-57. 1996
    ..Cells undergoing apoptosis in vivo showed increased release of cytochrome c to their cytosol, suggesting that mitochondria may function in apoptosis by releasing cytochrome c...
  98. pmc In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains
    T Fernandes-Alnemri
    Center for Apoptosis Research and the Kimmel Cancer Center Institute, Jefferson Medical College, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 93:7464-9. 1996
    ..These observations suggest that CPP32 and Mch3 are targets of mature Mch4 protease in apoptotic cells...
  99. ncbi The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32
    S M Srinivasula
    Center for Apoptosis Research, the Department of Biochemistry and Molecular Pharmacology, and the Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 271:27099-106. 1996
    Recent evidence suggests that CPP32 is an essential component of an aspartate-specific cysteine protease (ASCP) cascade responsible for apoptosis execution in mammalian cells...
  100. ncbi DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis
    X Liu
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 89:175-84. 1997
    ..In cells undergoing apoptosis, the 45 kDa subunit is cleaved in the same pattern as it is cleaved by caspase-3 in vitro. These data delineate a direct signal transduction pathway during apoptosis: caspase-3 to DFF to DNA fragmentation...
  101. ncbi Casper is a FADD- and caspase-related inducer of apoptosis
    H B Shu
    Tularik, Incorporated, South San Francisco, California 94080, USA
    Immunity 6:751-63. 1997
    ..When overexpressed in mammalian cells, Casper potently induces apoptosis. A C-terminal deletion mutant of Casper inhibits TNF- and Fas-induced cell death, suggesting that Casper is involved in these apoptotic pathways...

Research Grants86

  1. Regulation of caspase-1 by Sod2 in the heart
    Kumuda C Das; Fiscal Year: 2010
    ..The outcome of these studies may help in the therapeutic use of MnSOD or substances that induce MnSOD in the protection of myocardial injury in heart failure, endothelial dysfunction or other cardiovascular diseases. ..
  2. Apoptosis Execution Pathways in Dopaminergic Cell Death
    Jun Chen; Fiscal Year: 2007
    ..3. Test the hypotheses that Bax is a direct mediator for AIF release, and Bax contributes to dopaminergic cell death by activating both AIF-dependent and caspase-dependent pathways. ..
  3. Role of The Jak-Stat Pathway in Ischemic Preconditioning
    Yu Ting Xuan; Fiscal Year: 2010
    ..This proposal will provide important new insights into the signaling and molecular mechanisms whereby the late phase of ischemic PC reprograms the heart in the intact animal. ..
  4. Mechanisms and Modulation of Cell Death in Traumatic Brain Injury
    ALAN IRA FADEN; Fiscal Year: 2012
    ..A better understanding of the mechanisms underlying TBI would offer the possibility of improving survival and insuring a more complete recovery. ..
  5. David Piwnica-Worms; Fiscal Year: 2016
    ..These activities benefit from the combination expertise of this applicant team in chemistry, molecular imaging, biochemistry, and vision biology. ..
  6. Dhyan Chandra; Fiscal Year: 2016
    ..g., caspase-9, and -8). ..
  7. Apoptosis and Trophoblast Fusion
    NEAL STEWART ROTE; Fiscal Year: 2010
    ..In our most recent preliminary studies, we observed that decreased transcription and translation of caspase 3 and caspase 8 and increased expression of caspase 14 were concurrent with differentiation/syncytialization...
  8. Mechanistic targets for intervention in sepsis-induced renal injury
    Philip R Mayeux; Fiscal Year: 2012
    ..They will identify new signaling pathways and test them as new therapeutic targets for this devastating disease. ..
  9. Nucleotide Regulation of Apaf-1 Apoptosome and Apoptosis
    Dean G Tang; Fiscal Year: 2010
    ..The accomplishment of the proposed goals will greatly advance our understanding of apoptosome regulation in vivo and help us understand cellular response to environmental stress and cancer cell response to anti- tumor therapeutics. ..
  10. The Role of Caspases in Warm and Cold Ischemia
    ALKESH HARIHAR JANI; Fiscal Year: 2010
    ..Reduced MHC expression is associated with decreased kidney graft immunogenicity and has important implications for the deleterious effect of DGF on both short and long-term allograft function. ..
  11. The role of XIAP during cold ischemia and kidney transplantation.
    ALKESH HARIHAR JANI; Fiscal Year: 2013
    ..Specific Aim 2 investigates whether reducing RTEC apoptosis by manipulation of XIAP during CI before kidney transplant, improves graft function after transplant. ..
  12. Peixin Yang; Fiscal Year: 2016
    ..embryopathy and its activation leads to activation of Bcl-2 family members and effector caspases such as caspase 3. We further define the transcription factor and the apoptotic gene mediating diabetes-induced caspase 8 ..
  13. NON-APOPTOTIC ACTION OF CASPASES IN PLURIPOTENT EMBRYONIC STEM CELLS
    Thomas P Zwaka; Fiscal Year: 2012
    ..Specific pharmacological alteration of caspases may be useful not only for modulating programmed cell death, but also for directing stem cell fate. ..
  14. Shawn B Bratton; Fiscal Year: 2014
    ....
  15. Apoptotic Signaling Induces Hyperpermeability Following Hemorrhagic Shock
    Ed W Childs; Fiscal Year: 2010
    ..Atomic force microscopy will be used to assess endothelial cell adhesive forces. (End of Abstract) ..
  16. Novel Insight on Nucleotide Regulation of Apoptosome Activation
    Dhyan Chandra; Fiscal Year: 2010
    ..The newly obtained knowledge should also have important implications in helping to design novel anti-cancer therapeutics targeting the apoptotic machinery. ..
  17. Mechanisms of Increasing Apoptosis Regulation in Developing and Mature Neurons
    Mohanish Deshmukh; Fiscal Year: 2011
    ..Understanding the mechanism by which apoptosis is regulated in neurons is clinically important for identifying appropriate drug targets that would effectively prevent neuronal death after injury or disease. ..
  18. JEANNE ANN HARDY; Fiscal Year: 2016
    ..We focus on caspase proteins, which can cause cancer cells to die. ..
  19. Molecular Mechanisms of ApoE4 Proteolysis in Alzheimer's Disease
    Troy T Rohn; Fiscal Year: 2013
    ..Both immunohistochemical analysis using fixed post mortem brain sections as well as Western blot analysis from AD samples will be performed and compared to age-matched controls. ..
  20. CASPASES IN RENAL TUBULAR EPITHELIAL CELL INJURY
    GUR KAUSHAL; Fiscal Year: 2000
    ..These studies will provide new insights into our understanding of the cellular and molecular mechanisms of ischemic renal injury as well as other forms of acute renal failure. ..
  21. Chuan Yuan Li; Fiscal Year: 2015
    ..Our hypothesis is based on our recent discovery of the "Phoenix Rising" pathway through which caspase 3 activates paracrine signaling cascades from dying cells to stimulate tissue regeneration and the rapid ..
  22. Molecular intersection between neuronal apoptosis and axon degeneration
    COREY CUSACK; Fiscal Year: 2013
    ..This aim will also test the hypothesis that XIAP, an endogenous inhibitor of caspases, protects the neuronal cell body by restricting caspase activity to the axon during axonal degeneration. ..
  23. Biochemical characterization of caspase-3 activation
    Xiaodong Wang; Fiscal Year: 2004
    ..Such knowledge should provide guidance to the diagnosis and therapy of the common human diseases listed above. ..
  24. The Cyto-Protective Role of Hexokinase II During Metabolic Stress
    JONATHAN MICHAEL GALL; Fiscal Year: 2012
    ..decreased HKII or DRP1expression on mitochondrial injury and apoptosis will be studied by measuring AIF leakage, caspase 3 activation, annexin V staining in cells subjected to metabolic stress...
  25. Reactivating Apoptosis in Refractory Cancer by Selective Targeting of MCL-1
    Michelle Stewart; Fiscal Year: 2010
    ..pro-apoptotic stimuli will be evaluated in MCL-1-dependent cancer cell lines by monitoring cell viability and caspase 3/7 activation...
  26. Innate Immune Mechanisms of Motor Neuron Injury
    Timothy Vartanian; Fiscal Year: 2010
    ..We have identified a receptor termed TLR3 present on all neurons, that when activated, induces death to only motor neurons. This application pursues research to define the relevance of this finding to diseases of the motor neuron. ..
  27. Frank A Anania; Fiscal Year: 2016
    ..Additional data indicate that exendin-4 suppresses CHOP expression both in vitro and in mice fed a high-fat diet, but in CHOP knockout mice fed an identical diet, fat-laden hepatocytes are protected from apoptotic death. ..
  28. Estrogen: Neuroprotection in the Perimenopause
    Anne M Etgen; Fiscal Year: 2010
    ..element binding protein at early times after ischemia;2) the anti-apoptotic gene Bcl-2 and activation of caspase 3 at later times after ischemia;3) inactivation of Akt and subsequent activation of the forkhead transcription ..
  29. Discovery Tools for Chemotherapy Resistance to Cell Death.
    Robert W Sobol; Fiscal Year: 2012
    ....
  30. HAX-1: a Multifaceted Family of Apoptotic Regulators
    Aikaterini Kontrogianni-Konstantopoulos; Fiscal Year: 2012
    ..abstract_text> ..
  31. Jeffrey D Hasday; Fiscal Year: 2016
    ..The pharmacologic studies in aim 4 will provide new preclinical data that may lead to future therapies in patients with ALI. ..
  32. Radiosensitization via Targeting Cell Death
    Bo Lu; Fiscal Year: 2010
    ..In the absence of pro-apoptotic proteins, Bak/Bax or caspase 3/7, we found that autophagy is upregulated and occurred in over 50% of irradiated cells...
  33. ANUMANTHA GOUNDER KANTHASAMY; Fiscal Year: 2015
    ..The proposed work has translational potential, as neuroprotective strategies targeting the proposed signaling pathway may prove to be effective against PD. ..
  34. Sandra M Mooney; Fiscal Year: 2014
    ..during the period of naturally occurring neuronal death causes a dramatic and transient increase in both active caspase 3 expression and terminal uridylated nick-end labeling (TUNEL)...
  35. Caspase mediated cell death after brain trauma
    Ramesh Raghupathi; Fiscal Year: 2006
    ..abstract_text> ..
  36. Combination drug treatment to inhibit multiple cell death pathways after TBI
    ALAN IRA FADEN; Fiscal Year: 2010
    ..sites, thereby neutralizing their pro- apoptotic functions by preventing the formation of the apoptosome (and caspase 3) and attenuating AIF mediated actions...
  37. Oxidative Stress, PKC-delta Activation and Striatal Ischemic Cell Death
    Arthi Kanthasamy; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  38. Protein Nitration in the Placenta
    Leslie Myatt; Fiscal Year: 2012
    ..nitration and the role of hypoxia and hypoxia/reoxygenation on protein nitration and the apoptotic cascade (P53, caspase 3 and Bcl-2) will be investigated using pharmacologic, knockdown by siRNA and overexpression approaches in ..
  39. Randomized trial of PEITC as a modifier of NNK metabolism in smokers
    Jian Min Yuan; Fiscal Year: 2012
    ..The major strengths include the need for a randomized, placebo-controlled trial of PEITC, the qualification of the investigators, and the feasibility of the proposed studies. ..
  40. Growth Factor and Antiapoptotic Gene Delivery to Human Islets
    Ram I Mahato; Fiscal Year: 2012
    ..This will decrease the number of islets required to achieve normoglycemia in type I diabetic patients. ..
  41. Human Cardiomyopathy and HSP60
    Anne A Knowlton; Fiscal Year: 2012
    ..the cardiac myocyte in a third of cells;the presence of HSP60 on the cell surface correlated with activation of caspase 3, 7 and 8...
  42. Christopher W Akey; Fiscal Year: 2015
    ..When taken together, these Aims should reveal new aspects of apoptosome function that are germane to human health and disease. ..
  43. Regulation of Oxidative Stress Induced Apoptosis in Osteoblasts
    GABRIEL ROBERT LINARES; Fiscal Year: 2010
    ..mitochondrial dependent apoptosis pathway and 2) Demonstrate that Grx5 is a modulator of cytochrome c release, caspase 3, 9 activation and subsequent PARP cleavage...
  44. Zhaoyong Hu; Fiscal Year: 2016
    ..The results of our study might be used to define cell biologic responses that are interrupted by CKD. The results also might yield the initial steps towards designing new therapies for this dreaded complication of CKD. . ..
  45. Alcohol and Sca-1;Impairment of the granulopoietic precursor cell response
    John Nicholas Melvan; Fiscal Year: 2013
    ..It will also build a foundation for developing effective therapeutic strategies to treat myelosuppression in alcohol abusers with severe bacterial infection. ..
  46. A novel mouse prostate carcinogenesis model for dietary cancer prevention researc
    Chung S Yang; Fiscal Year: 2013
    ....
  47. Regulation of Apoptotic Signaling Pathways by Alpha-Crystallins in the Ocular Len
    David W Li; Fiscal Year: 2010
    ..abstract_text> ..
  48. Ping Zhang; Fiscal Year: 2014
    ..This project investigates the mechanisms underlying this serious health problem and will help to identify therapeutic approaches for effective treatment of alcoholic patients with severe bacterial infection. ..
  49. Mechanisms of Angiogenesis in Retinopathy of Prematurity
    Mary Elizabeth Hartnett; Fiscal Year: 2009
    ..g., dihydroethidium);and NADPH oxidase activation. ..
  50. MARY ELIZABETH RUTH HARTNETT; Fiscal Year: 2016
    ..g., dihydroethidium);and NADPH oxidase activation. ..
  51. Chemoprevention of Pancreatic Cancer by Capsaicin
    Sanjay K Srivastava; Fiscal Year: 2013
    ..Successful completion of this project will support the development and clinical application of capsaicin for the chemoprevention of pancreatic cancer. ..
  52. Resham Bhattacharya; Fiscal Year: 2016
    ..The proposed studies will validate Bmi-1 as an important new target using readily translatable nanoliposomal delivery methods with potential for therapy even in chemoresistant ovarian cancer. ..
  53. Neurotoxicity of Magnetic Nanoparticles
    Veronica Shubayev; Fiscal Year: 2010
    ..influx of macrophages, activation of heme oxygenase-1, interleukin-12, matrix metalloproteinase (MMP)-9 and caspase 3, all consistent with the oxidative stress paradigm...
  54. Induced Reactive Oxygen Species in a 3-D Human Pancreatitis Model in Vitro
    Gregory P Botta; Fiscal Year: 2013
    ..abstract_text> ..
  55. John H Zhang; Fiscal Year: 2014
    ..The clinical implication of HBO-PC, as an effective, easy to use, and safe modality, could be expanded to multiple brain injuries complicating major surgical procedures. ..
  56. LIPID MODULATORS OF PULMONARY VASCULAR TONE
    Elizabeth R Jacobs; Fiscal Year: 2010
    ..anti-apoptotic activity of 20-HETE in PAECs, with 20-HETE protecting against starvation-evoked increases in caspase 3 activity...
  57. A cell-specific inducible model of hearing loss
    Albert S Edge; Fiscal Year: 2011
    ..Because of the clear correlation between loss of cochlear cells and sensorineural hearing loss, improvements in our ability to regenerate these cells will lead directly to new treatments for loss of hearing. ..
  58. Cell Cycle Pathways and Spinal Cord Injury
    ALAN IRA FADEN; Fiscal Year: 2011
    ..therapeutically relevant, delayed systemic administration of Flavopiridol improves post-traumatic function and histological outcome. ..
  59. Cellular and molecular mechanisms of gp120 neurotoxicity
    Italo Mocchetti; Fiscal Year: 2012
    ..Overall, we expect to provide new significant data that help in the design of adjunct therapies against synaptic simplifications caused by HIV. ..
  60. Targeting induction of calcium buffer proteins for treatment of viral encephaliti
    John D Morrey; Fiscal Year: 2013
    ..that WNV infection in different cell lines leads to Ca++ influx, which benefits is replication, but induces caspase 3 cleavage and increase the risk of cell death...
  61. NEUROCHEMICAL PHARMACOLOGY OF PHENCYCLIDINE
    Kenneth Johnson; Fiscal Year: 2009
    ..The potential role of CREB dephosphorylation as a result of over activation of NMDA receptors will also be assessed in the neurotoxicity of PCP treatment. ..
  62. Downstream Regulators of Beta-Amyloid Induced Neuronal Death
    Carol M Troy; Fiscal Year: 2010
    ..2: To determine how caspase-2 is regulated and activated by A_ and TFD. 3: To determine how caspase-2 induces of Bim expression. ..
  63. ELIZABETH ANN EKLUND; Fiscal Year: 2016
    ..Xiap inhibits caspase 3, contributing to Fas-resistance. We identified RASSF5, the gene encoding Nore1, as another Icsbp-target-gene...
  64. Kenneth L Tyler; Fiscal Year: 2015
    ..Inhibition of caspase 3, the executioner caspase of apoptotic cell death, results in decreased severity of virus-induced encephalitis...
  65. Inhibition of S14 by Conjugated Linoleic Acid in Advanced Solid Tumor Patients
    Raymond P Perez; Fiscal Year: 2010
    ..as FAS), SREBP1, phospho-AKT, biomarkers of cell-cycle/proliferation (Ki67, geminin), and apoptosis (cleaved caspase 3)...
  66. White matter changes in HIV models
    Pamela E Knapp; Fiscal Year: 2011
    ..Culture studies with viral protein and HIV test whether oligodendrocytes are directly affected. ..
  67. Jia Luo; Fiscal Year: 2016
    ..The current study is to determine whether C3G ameliorates ethanol-induced neuronal loss as well as behavioral deficits. The proposal will potentially develop a new therapeutic strategy. ..
  68. Edward D Chan; Fiscal Year: 2015
    ..Additionally, enhancing the bioactivity of IL-32 should increase induction of PCD and MTB killing. ..
  69. Whole brain multimodal microscopy of an apoptosis reporter mouse
    PETER JEFF NICHOLLS; Fiscal Year: 2013
    ....
  70. Characterization of prostatic stem cells and prostate cancer-initiating cells
    Li Xin; Fiscal Year: 2012
    ..The infected cells will be microinjected into immunodeficient host mouse prostate or tested in the regeneration system to determine their capacity to initiate cancer. ..
  71. Li Tang; Fiscal Year: 2015
    ..Due to the wide availability of cruciferous vegetables and the high diet-change motivation of breast cancer patients, the findings from the study may have a significant impact on the lifestyle of breast cancer survivors. ..
  72. Davide Trotti; Fiscal Year: 2015
    ..The scope of this last aim could then be key, in particular if results of aim 1-2 establisha role of the astroglial CTE-SUMO1 fragment in motor neuron toxicity and ALS pathogenesis. ..
  73. Jena J Steinle; Fiscal Year: 2016
    ..In addition to preventing degenerate capillaries in vivo, compound 49b prevents the cleavage of caspase 3, a well- established marker of apoptosis, in retinal endothelial cells (REC) in vitro, suggesting that Compound ..
  74. Salmonella Pathogenesis and Processing of Secreted Effectors by Caspase-3
    Beth A McCormick; Fiscal Year: 2013
    ..Speculatively, the ability of the organism to hijack the host CASP3 machinery in order to activate secreted effectors is likely to be a general paradigm employed by enteric pathogens that harbor a T3SS to cause disease. ..
  75. Mechanisms of morphine mediated neuroprotection against gp120Bal toxicity.
    Lee Campbell; Fiscal Year: 2013
    ..Moreover the mechanistic studies proposed will likely identify intermediaries which can be targeted for the development of future therapeutics. ..
  76. Kathryn J Grive; Fiscal Year: 2015
    ..At birth, TAF4b deficient ovaries appear to lose oocytes through caspase 3-dependent apoptosis, which likely leads to the ovarian senescence observed in reproductively mature mice...
  77. Denise J Montell; Fiscal Year: 2014
    ..The proposed work has the potential to lead to a new understanding of and treatments for degenerative diseases and cancer. ..
  78. Diagnosing Emergence of Kinase Inhibitor Resistance on a Microchip
    Thomas G Graeber; Fiscal Year: 2013
    ..Graeber, H.-R. Tseng, R. Lo and A. Ribas), make the melanoma microfluidic diagnostic approach uniquely positioned to impact ongoing clinical trials and therapy. ..
  79. The 5-HT1A Receptor and Brain Development
    Probal Banerjee; Fiscal Year: 2005
    ..This project will delineate a novel pathway that could play a key role in early brain development. Knowledge of this pathway will help in designing better therapies to combat developmental brain disorders. ..
  80. NEUROPROTECTIVE STRATEGIES AGAINST OXIDATIVE DAMAGE
    Anumantha Kanthasamy; Fiscal Year: 2009
    ....
  81. Tomoko Makishima; Fiscal Year: 2016
    ..When the proposed studies have been completed, it is our expectation that we will be able to compare basic results of vestibular dysfunction in mice and in human. ..
  82. Piero Anversa; Fiscal Year: 2015
    ..Ultimately, CSC/progenitor cell therapy will be established on an individual basis to maximize the efficacy of this novel approach for the management of heart failure in each case. ..
  83. Clinical Research Consortium for Spinocerebellar Ataxias
    Tetsuo Ashizawa; Fiscal Year: 2010
    ..Initiate a pilot study to determine genetic modifiers of SCA 1, 2, 3 and 6 Aim 4...

Patents1

  1. Therapeutic system targeting pathogen proteases and uses thereof
    Patent Number: WO2004019990-A1; Date:2004-03-11