BTRC

Summary

Gene Symbol: BTRC
Description: beta-transducin repeat containing E3 ubiquitin protein ligase
Alias: BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP, F-box/WD repeat-containing protein 1A, E3RSIkappaB, F-box and WD repeats protein beta-TrCP, F-box and WD-repeat protein 1B, beta-TrCP1, epididymis tissue protein Li 2a, pIkappaBalpha-E3 receptor subunit
Species: human

Top Publications

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
  2. pmc Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
  3. doi An insertion/deletion polymorphism in the 3' untranslated region of beta-transducin repeat-containing protein (betaTrCP) is associated with susceptibility for hepatocellular carcinoma in Chinese
    Shougong Chen
    Department of Forensic Medicine, Medical College of Soochow University, Suzhou, 215123 Jiangsu, PR China
    Biochem Biophys Res Commun 391:552-6. 2010
  4. pmc Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
  5. pmc A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells
    Shuo Wei
    Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 283:26759-70. 2008
  6. pmc The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCFβ-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition
    Wei Huang
    Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    J Biol Chem 287:26245-53. 2012
  7. pmc Structural and functional characterization of Nrf2 degradation by the glycogen synthase kinase 3/β-TrCP axis
    Patricia Rada
    Departamento de Bioquímica e Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Centro de Investigación en Red sobre Enfermedades Neurodegenerativas, Madrid, Spain
    Mol Cell Biol 32:3486-99. 2012
  8. ncbi RASSF1C, an isoform of the tumor suppressor RASSF1A, promotes the accumulation of beta-catenin by interacting with betaTrCP
    Emilie Estrabaud
    Institut Cochin, Département Maladies Infectieuses Institut National de la Sante et de la Recherche Medicale U567, 27 rue du Faubourg Saint Jacques, Bâtiment G Roussy, 75014 Paris, France
    Cancer Res 67:1054-61. 2007
  9. ncbi A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
    F Margottin
    CJF 97 03 INSERM, Institut Cochin de Genetique Moleculaire, Universite Paris V, Faculté de Médecine Cochin, France
    Mol Cell 1:565-74. 1998
  10. ncbi Split-hand/split-foot malformation 3 (SHFM3) at 10q24, development of rapid diagnostic methods and gene expression from the region
    Robert Lyle
    Department of Genetic Medicine and Development, University of Geneva Medical School, 1 rue Michel Servet, 1211 Geneva, Switzerland
    Am J Med Genet A 140:1384-95. 2006

Research Grants

  1. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2013
  2. Genetic Analysis of Snail Superfamily Genes in Mice
    THOMAS HOOKER GRIDLEY; Fiscal Year: 2012
  3. Wenyi Wei; Fiscal Year: 2015
  4. NF-kappaB in myeloma cell growth and survival in vivo
    Babatunde Oyajobi; Fiscal Year: 2009
  5. SNAIL-Dependent Regulation of EMT in Cancer
    STEPHEN JOSEPH WEISS; Fiscal Year: 2010
  6. Stability of prolactin receptor and prolactin signaling
    Serge Y Fuchs; Fiscal Year: 2010
  7. JAROSLAW WALDEMAR ZMIJEWSKI; Fiscal Year: 2015
  8. Gli Control of PTH-rP and Osteolysis in Breast Cancer
    Gregory Mundy; Fiscal Year: 2009
  9. Vladimir S Spiegelman; Fiscal Year: 2016
  10. Regulation of PTHrP expression in chondrocytes by Gli2
    JULIE STERLING; Fiscal Year: 2006

Scientific Experts

Detail Information

Publications193 found, 100 shown here

  1. ncbi IkappaBalpha ubiquitination is catalyzed by an SCF-like complex containing Skp1, cullin-1, and two F-box/WD40-repeat proteins, betaTrCP1 and betaTrCP2
    H Suzuki
    Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Ibaraki, Tsukuba shi, 305 8585, Japan
    Biochem Biophys Res Commun 256:127-32. 1999
    ..These results indicate that the long-sought IkappaBalpha-E3 is an SCF-like complex consisting of multiple proteins which are coordinately assembled during phosphorylation of IkappaBalpha in response to external signals...
  2. pmc Serine-threonine ubiquitination mediates downregulation of BST-2/tetherin and relief of restricted virion release by HIV-1 Vpu
    Andrey A Tokarev
    Department of Medicine University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0679, USA
    J Virol 85:51-63. 2011
    ..Serine-threonine ubiquitination of BST-2 is likely part of the mechanism by which Vpu counteracts innate defenses...
  3. doi An insertion/deletion polymorphism in the 3' untranslated region of beta-transducin repeat-containing protein (betaTrCP) is associated with susceptibility for hepatocellular carcinoma in Chinese
    Shougong Chen
    Department of Forensic Medicine, Medical College of Soochow University, Suzhou, 215123 Jiangsu, PR China
    Biochem Biophys Res Commun 391:552-6. 2010
    ..As a positive regulator of NFkappaB signaling pathway, beta-transducin repeat-containing protein (betaTrCP) is overexpressed and oncogenic in epithelial cancers, suggesting a potential role of betaTrCP in HCC ..
  4. pmc Multilayered mechanism of CD4 downregulation by HIV-1 Vpu involving distinct ER retention and ERAD targeting steps
    Javier G Magadán
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1000869. 2010
    ..The multiple levels at which Vpu engages these cellular quality control mechanisms underscore the importance of ensuring profound suppression of CD4 to the life cycle of HIV-1...
  5. pmc A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells
    Shuo Wei
    Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 283:26759-70. 2008
    ..Moreover, we obtained evidence that this beta-TrCP-dependent degradation takes part in controlling cyclin D1 turnover when cancer cells undergo glucose starvation, which endows physiological relevance to this novel mechanism...
  6. pmc The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCFβ-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition
    Wei Huang
    Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
    J Biol Chem 287:26245-53. 2012
    ..This study provides a novel mechanism of TAZ regulation and suggests a role of TAZ in modulating tissue growth and tumor development in response to PI3K signaling...
  7. pmc Structural and functional characterization of Nrf2 degradation by the glycogen synthase kinase 3/β-TrCP axis
    Patricia Rada
    Departamento de Bioquímica e Instituto de Investigaciones Biomédicas Alberto Sols UAM CSIC, Centro de Investigación en Red sobre Enfermedades Neurodegenerativas, Madrid, Spain
    Mol Cell Biol 32:3486-99. 2012
    ..This study establishes the structural parameters of the interaction of Nrf2 with the GSK-3/β-TrCP axis and its functional relevance in the regulation of Nrf2 by the signaling pathways that impinge on GSK-3...
  8. ncbi RASSF1C, an isoform of the tumor suppressor RASSF1A, promotes the accumulation of beta-catenin by interacting with betaTrCP
    Emilie Estrabaud
    Institut Cochin, Département Maladies Infectieuses Institut National de la Sante et de la Recherche Medicale U567, 27 rue du Faubourg Saint Jacques, Bâtiment G Roussy, 75014 Paris, France
    Cancer Res 67:1054-61. 2007
    ..Here, we show that RASSF1C, but not RASSF1A, interacts with betaTrCP. Binding of RASSF1C to betaTrCP involves serine 18 and serine 19 of the SS(18)GYXS(19) motif present in RASSF1C ..
  9. ncbi A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
    F Margottin
    CJF 97 03 INSERM, Institut Cochin de Genetique Moleculaire, Universite Paris V, Faculté de Médecine Cochin, France
    Mol Cell 1:565-74. 1998
    ..Human beta TrCP identified by interaction with Vpu connects CD4 to this proteolytic machinery, and CD4-Vpu-beta TrCP ternary ..
  10. ncbi Split-hand/split-foot malformation 3 (SHFM3) at 10q24, development of rapid diagnostic methods and gene expression from the region
    Robert Lyle
    Department of Genetic Medicine and Development, University of Geneva Medical School, 1 rue Michel Servet, 1211 Geneva, Switzerland
    Am J Med Genet A 140:1384-95. 2006
    ..FISH and quantitative PCR that SHFM3 is caused by a minimal 325 kb duplication containing only two genes (BTRC and POLL)...
  11. doi Oncogene-mediated inhibition of glycogen synthase kinase 3 beta impairs degradation of prolactin receptor
    Alexander Plotnikov
    Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104 4539, USA
    Cancer Res 68:1354-61. 2008
    ..breast cancer due to decreased phosphorylation of residue Ser(349), which, when phosphorylated, recruits the beta Trcp E3 ubiquitin ligase and facilitates PRLr degradation...
  12. pmc Thiazolidinediones mimic glucose starvation in facilitating Sp1 degradation through the up-regulation of beta-transducin repeat-containing protein
    Shuo Wei
    Division of Medicinal Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA
    Mol Pharmacol 76:47-57. 2009
    ..The ability of OSU-CG12 to mimic glucose starvation to activate beta-TrCP-mediated Sp1 degradation has translational potential to foster novel strategies for cancer therapy...
  13. pmc An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin
    M Kitagawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    EMBO J 18:2401-10. 1999
    ..Here we show that FWD1 (the mouse homologue of Slimb/betaTrCP), an F-box/WD40-repeat protein, specifically formed a multi-molecular complex with beta-catenin, Axin, GSK-3beta ..
  14. ncbi Preferential interaction of TIP120A with Cul1 that is not modified by NEDD8 and not associated with Skp1
    Kiyotaka Oshikawa
    Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Japan
    Biochem Biophys Res Commun 303:1209-16. 2003
    ..These observations thus suggest that TIP120A may function as a negative regulator of the SCF complex by binding to nonneddylated Cul1 and thereby preventing assembly of this ubiquitin ligase...
  15. pmc Silencing of both beta-TrCP1 and HOS (beta-TrCP2) is required to suppress human immunodeficiency virus type 1 Vpu-mediated CD4 down-modulation
    Christophe Butticaz
    Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland
    J Virol 81:1502-5. 2007
    ....
  16. ncbi Involvement of the betaTrCP in the ubiquitination and stability of the HIV-1 Vpu protein
    Nadia Belaïdouni
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Biophys Res Commun 357:688-93. 2007
    ..This process requires the recruitment of human betaTrCP, a component of the Skp1-Cullin-F box (SCF) ubiquitin ligase complex, that interacts with phosphorylated Vpu ..
  17. doi Translation inhibitor Pdcd4 is targeted for degradation during tumor promotion
    Tobias Schmid
    Laboratory of Cancer Prevention, National Cancer Institute, Frederick, MD 21702, USA
    Cancer Res 68:1254-60. 2008
    ..Thus, because endogenous Pdcd4 levels are limiting for tumorigenesis, inhibiting signaling to Pdcd4 degradation may prove a valid strategy for cancer prevention and intervention...
  18. ncbi The ubiquitin ligase SCF(betaTrCP) regulates the degradation of the growth hormone receptor
    Peter van Kerkhof
    Department of Cell Biology, University Medical Center Utrecht and Institute of Biomembranes, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 282:20475-83. 2007
    ..Together, these findings provide direct evidence for a key role of the SCF(TrCP) in the endocytosis and degradation of an important factor in growth, immunity, and life span regulation...
  19. pmc BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1
    Aurore Keutgens
    Interdisciplinary Cluster for Applied Genoproteomics, GIGA Research, Unit of Medical Chemistry, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Sart Tilman, 4000 Liege, Belgium
    J Biol Chem 285:25831-40. 2010
    ..Thus, our data defined a unique motif of BCL-3 that is needed for its recruitment to the proteasome and identified PSMB1 as a key protein required for the proteasome-mediated degradation of a nuclear and oncogenic IkappaB protein...
  20. ncbi beta-TrCP mediates the signal-induced ubiquitination of IkappaBbeta
    C Wu
    Section of Immunobiology, Department of Molecular Biophysics, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Biol Chem 274:29591-4. 1999
    ..Therefore, these results indicate that beta-TrCP plays a critical role in the activation of NF-kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta...
  21. pmc The human immunodeficiency virus type 1 accessory protein Vpu induces apoptosis by suppressing the nuclear factor kappaB-dependent expression of antiapoptotic factors
    H Akari
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 194:1299-311. 2001
    Human immunodeficiency virus (HIV) type 1 Vpu is an integral membrane protein with a unique affinity for betaTrCP (TrCP), a key member of the SkpI-Cullin-F-box E3 ubiquitin ligase complex that is involved in the regulated degradation of ..
  22. ncbi High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML
    Manuel Favre
    Division of Infectious Diseases and Institute of Microbiology, University Hospital of Lausanne, Switzerland
    J Acquir Immune Defic Syndr 34:127-33. 2003
    ..6 human genes were investigated-tumor suppressor gene 101 (TSG101), beta-transducin repeats containing protein (betaTrCP), peptidyl-proly cis-trans isomerase, cyclophilin A (PPIA), integrase interactor 1 protein (INI1), Nef-associated ..
  23. pmc M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP
    Nobumoto Watanabe
    Antibiotics Laboratory, Discovery Research Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:4419-24. 2004
    ..These results also establish the existence of a feedback loop between Cdc2 and Wee1A in somatic cells that depends on ubiquitination and protein degradation and ensures the rapid activation of Cdc2 when cells are ready to divide...
  24. ncbi Overexpression of human beta TrCP1 deleted of its F box induces tumorigenesis in transgenic mice
    Nadia Belaïdouni
    Department of Infectious Diseases, Department of INSERM U567, Institut Cochin, CNRS UMR8104, Université R Descartes Paris V, 27 rue du Faubourg Saint Jacques, 75014 Paris, France
    Oncogene 24:2271-6. 2005
    ..the synthesis of unphosphorylated beta-catenin that escapes recognition by the beta transducin repeat protein (beta TrCP1), the receptor of an ubiquitin...
  25. ncbi NMR studies for identifying phosphopeptide ligands of the HIV-1 protein Vpu binding to the F-box protein beta-TrCP
    Nathalie Evrard-Todeschi
    Universite Rene Descartes Paris V, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques UMR 8601 CNRS, 45 rue des Saint Peres, 75270 Paris Cedex 06, France
    Peptides 27:194-210. 2006
    ..These findings are in good agreement with a recently published X-ray structure of a shorter beta-Catenin fragment with the beta-TrCP complex...
  26. ncbi Gli2 is targeted for ubiquitination and degradation by beta-TrCP ubiquitin ligase
    Neehar Bhatia
    Department of Dermatology, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA
    J Biol Chem 281:19320-6. 2006
    ..These data identify beta-TrCP2 as a pivotal regulator of Gli2 expression and point to an important role for posttranslational modulation of GLI2 protein levels in Hh pathway-associated human prostate cancer...
  27. ncbi Constitutive and UV-induced fibronectin degradation is a ubiquitination-dependent process controlled by beta-TrCP
    Dipankar Ray
    Department of Molecular Pharmacology and Biological Chemistry, Robert H Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 281:23060-5. 2006
    ..Taken together, constitutive FN degradation, as well as UV-induced degradation, is ubiquitination dependent and controlled by beta-TrCP...
  28. ncbi Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress
    Niels Mailand
    Institute of Cancer Biology and Centre for Genotoxic Stress Research, Danish Cancer Society, Strandboulevarden 49, DK 2100, Copenhagen, Denmark
    Mol Cell 23:307-18. 2006
    ..interaction was phosphorylation dependent and required the activity of the Plk1 kinase and the integrity of a betaTrCP recognition motif (phosphodegron) in the N terminus of Claspin...
  29. ncbi Phosphorylation-dependent regulation of stability and transforming potential of ETS transcriptional factor ESE-1 by p21-activated kinase 1
    Bramanandam Manavathi
    Department of Molecular and Cellular Oncology, University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    J Biol Chem 282:19820-30. 2007
    ..These findings provide novel insights into the mechanism of transformation potential of ESE-1 and discovered that ESE-1 functions are coordinately regulated by Pak1 phosphorylation and beta-TrCP-dependent ubiquitin-proteasome pathways...
  30. ncbi Identification of the receptor component of the IkappaBalpha-ubiquitin ligase
    A Yaron
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Nature 396:590-4. 1998
    ..This component, which we denote E3RSIkappaB (pIkappaBalpha-E3 receptor subunit), binds specifically to pIkappaBalpha and promotes its in vitro ..
  31. pmc The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro
    J T Winston
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:270-83. 1999
    ..Our results suggest that an SCFbeta-TRCP complex functions in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway...
  32. pmc Signal-induced ubiquitination of IkappaBalpha by the F-box protein Slimb/beta-TrCP
    E Spencer
    Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9148 USA
    Genes Dev 13:284-94. 1999
    ..These biochemical and genetic data strongly suggest that Slimb/beta-TrCP is the specificity determinant for the signal-induced ubiquitination of IkappaBalpha...
  33. ncbi The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin
    E Latres
    Department of Pathology and Kaplan Comprehensive Cancer Center, New York University Medical Center, NY 10016, USA
    Oncogene 18:849-54. 1999
    ..These results indicate that the Cul1/Skp1/beta-Trcp complex forms a ubiquitin ligase that mediates the degradation of beta-catenin...
  34. ncbi A ubiquitin ligase complex essential for the NF-kappaB, Wnt/Wingless, and Hedgehog signaling pathways
    T Maniatis
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138, USA
    Genes Dev 13:505-10. 1999
  35. ncbi The F-box protein beta-TrCP associates with phosphorylated beta-catenin and regulates its activity in the cell
    M Hart
    Onyx Pharmaceuticals 3031 Research Drive Richmond California 94806 USA
    Curr Biol 9:207-10. 1999
    ..In contrast, beta-TrCP2 did not associate with beta-catenin. We conclude that beta-TrCP is a component of an E3 ubiquitin ligase that is responsible for the targeted degradation of phosphorylated beta-catenin...
  36. ncbi Molecular dissection of the interactions among IkappaBalpha, FWD1, and Skp1 required for ubiquitin-mediated proteolysis of IkappaBalpha
    K Hattori
    Department of Molecular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 274:29641-7. 1999
    The SCF complex containing Skp1, Cul1, and the F-box protein FWD1 (the mouse homologue of Drosophila Slimb and Xenopus beta-TrCP) functions as the ubiquitin ligase for IkappaBalpha...
  37. ncbi Differential interaction of plakoglobin and beta-catenin with the ubiquitin-proteasome system
    E Sadot
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Oncogene 19:1992-2001. 2000
    ....
  38. pmc The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1
    M Furukawa
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Mol Cell Biol 20:8185-97. 2000
    ..This pathway may be commonly utilized for the assembly of other cullin ligases...
  39. pmc ATF4 degradation relies on a phosphorylation-dependent interaction with the SCF(betaTrCP) ubiquitin ligase
    I Lassot
    INSERM Unite 529, Interactions Moléculaires Hôte pathogène, 75014 Paris, France
    Mol Cell Biol 21:2192-202. 2001
    ..Here we show that the F-box protein betaTrCP, the receptor component of the SCF E3 ubiquitin ligase responsible for IkappaBalpha and beta-catenin degradation, ..
  40. ncbi Phosphorylation- and Skp1-independent in vitro ubiquitination of E2F1 by multiple ROC-cullin ligases
    T Ohta
    Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 27599 7295, USA
    Cancer Res 61:1347-53. 2001
    ..These results suggest a novel, SKP1-independent mechanism for targeting E2F1 ubiquitination...
  41. ncbi The human immunodeficiency virus type 1 Vpu protein inhibits NF-kappa B activation by interfering with beta TrCP-mediated degradation of Ikappa B
    S Bour
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 276:15920-8. 2001
    ..This process involves the recruitment of human betaTrCP (TrCP), a key member of the SkpI-Cdc53-F-box E3 ubiquitin ligase complex that specifically interacts with ..
  42. pmc Pseudosubstrate regulation of the SCF(beta-TrCP) ubiquitin ligase by hnRNP-U
    Matti Davis
    The Lautenberg Center for Immunology, The Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    Genes Dev 16:439-51. 2002
    ..Our study points to a novel regulatory mechanism, which secures the localization, stability, substrate binding threshold, and efficacy of a specific protein-ubiquitin ligase...
  43. ncbi Genetic evidence for the essential role of beta-transducin repeat-containing protein in the inducible processing of NF-kappa B2/p100
    Abraham Fong
    Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 277:22111-4. 2002
    ..Interestingly the constitutive processing of p100 mutants was independent of beta-TrCP. These results suggest that beta-TrCP is an essential component of NIK-induced p100 processing...
  44. ncbi NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases
    Jidong Liu
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 10:1511-8. 2002
    ..We suggest that by restricting SKP1-CUL1 interaction, CAND1 regulated the assembly of productive SCF ubiquitin ligases, allowing a common CUL1-ROC core to be utilized by a large number of SKP1-F box-substrate subcomplexes...
  45. ncbi TIP120A associates with cullins and modulates ubiquitin ligase activity
    Kyoeng Woo Min
    Department of Biochemistry and Protein Network Research Center, Yonsei University, Seoul 120 749, Korea
    J Biol Chem 278:15905-10. 2003
    ..These results suggest that TIP120A functions as a negative regulator of SCF E3 ubiquitin ligases and may modulate other cullin ligases in a similar fashion...
  46. ncbi Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
    Geng Wu
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:1445-56. 2003
    ....
  47. ncbi HIV-1 Vpu sequesters beta-transducin repeat-containing protein (betaTrCP) in the cytoplasm and provokes the accumulation of beta-catenin and other SCFbetaTrCP substrates
    Corinne Besnard-Guerin
    Institut Cochin, Department of Infectious Diseases, INSERM U567, CNRS UMR 8104, Université R Descartes Paris V, 27 rue du Faubourg St Jacques, 75014 Paris, France
    J Biol Chem 279:788-95. 2004
    ..adaptor for the proteasomal degradation of CD4 by recruiting CD4 and beta-transducin repeat-containing protein (betaTrCP), the receptor component of the multisubunit SCF-betaTrCP E3 ubiquitin ligase complex...
  48. ncbi Degradation of Cdc25A by beta-TrCP during S phase and in response to DNA damage
    Luca Busino
    European Institute of Oncology, 435 Via Ripamonti, 20141 Milan, Italy
    Nature 426:87-91. 2003
    ..Our results show that beta-TrCP has a crucial role in mediating the response to DNA damage through Cdc25A degradation...
  49. pmc SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase
    Jianping Jin
    Department of Pathology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Genes Dev 17:3062-74. 2003
    ..These data indicate that Cdc25A turnover is more complex than previously appreciated and suggest roles for an additional kinase(s) in Chk1-dependent Cdc25A turnover...
  50. pmc Role of F-box protein betaTrcp1 in mammary gland development and tumorigenesis
    Yasusei Kudo
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, NY 10016, USA
    Mol Cell Biol 24:8184-94. 2004
    ....
  51. ncbi Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition
    Binhua P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 6:931-40. 2004
    ..Furthermore, inhibition of GSK-3beta results in the upregulation of Snail and downregulation of E-cadherin in vivo. Thus, Snail and GSK-3beta together function as a molecular switch for many signalling pathways that lead to EMT...
  52. pmc Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1
    David V Hansen
    Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Mol Biol Cell 15:5623-34. 2004
    ..In prophase, phosphorylation of Emi1 generates a D-pS-G-X-X-pS degron to recruit the SCF(betaTrCP) ubiquitin ligase, causing Emi1 destruction and allowing progression beyond prometaphase, but the kinases ..
  53. pmc Transforming growth factor beta facilitates beta-TrCP-mediated degradation of Cdc25A in a Smad3-dependent manner
    Dipankar Ray
    Department of Biochemistry and Molecular Genetics, University of Illinois College of Medicine, Chicago, IL 60607, USA
    Mol Cell Biol 25:3338-47. 2005
    ..These data suggest that Smad3 plays a key role in the regulation of Cdc25A ubiquitination by SCFbeta-TrCP and that Cdc25A stabilization observed in various cancers could be associated with defects in the TGF-beta-Smad3 pathway...
  54. pmc Beta-TrCP recognizes a previously undescribed nonphosphorylated destruction motif in Cdc25A and Cdc25B phosphatases
    Yoshinori Kanemori
    Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
    Proc Natl Acad Sci U S A 102:6279-84. 2005
    ....
  55. ncbi SCFbeta-TRCP controls clock-dependent transcription via casein kinase 1-dependent degradation of the mammalian period-1 (Per1) protein
    Takahiro Shirogane
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:26863-72. 2005
    ....
  56. ncbi Stabilization of prolactin receptor in breast cancer cells
    Y Li
    Department of Animal Biology, University of Pennsylvania, Philadelphia, PA 19104 6046, USA
    Oncogene 25:1896-902. 2006
    ..These findings represent a novel mechanism through which altered PRLr stability may directly influence the pathogenesis of breast cancer...
  57. ncbi beta-TrCP binding and processing of NF-kappaB2/p100 involve its phosphorylation at serines 866 and 870
    Chunyang Liang
    Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey Medical Center, P O Box 850, Hershey, Pennsylvania 17033, USA
    Cell Signal 18:1309-17. 2006
    ..These data suggest that p100 processing involves its phosphorylation at specific terminal serines, which form a binding site for beta-TrCP thereby regulating p100 ubiquitination...
  58. ncbi Identification of substrates for F-box proteins
    Jianping Jin
    Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 399:287-309. 2005
    ..In addition, we describe approaches for the use of immobilized phosphopeptides to identify F-box proteins that recognize particular phosphodegrons...
  59. pmc Multisite protein kinase A and glycogen synthase kinase 3beta phosphorylation leads to Gli3 ubiquitination by SCFbetaTrCP
    Denis Tempé
    département génétique et développement, Institut Cochin, 24 rue du Faubourg St Jacques, 75014 Paris, France
    Mol Cell Biol 26:4316-26. 2006
    ..We identified multiple betaTrCP-binding sites related to the DSGX2-4S motif in Gli3, which are intertwined with PKA and GSK3beta sites, and ..
  60. ncbi SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response
    Angelo Peschiaroli
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, MSB 599, New York, New York 10016, USA
    Mol Cell 23:319-29. 2006
    ..We found that during recovery from the DNA replication checkpoint response, Claspin is degraded in a betaTrCP-dependent manner...
  61. ncbi S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth
    N Valerio Dorrello
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA
    Science 314:467-71. 2006
    ..rapidly phosphorylated on Ser67 by the protein kinase S6K1 and subsequently degraded via the ubiquitin ligase SCF(betaTRCP)...
  62. pmc SAG/ROC-SCF beta-TrCP E3 ubiquitin ligase promotes pro-caspase-3 degradation as a mechanism of apoptosis protection
    Mingjia Tan
    Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, 1500 East Medical Center Drive, Ann Arbor, MI 48109 0936, USA
    Neoplasia 8:1042-54. 2006
    ..Thus, pro-caspase-3 appears to be a substrate of SAG/ROC-SCF(beta-TrCP) E3 Ub ligase, which protects cells from apoptosis through increased apoptosis threshold by reducing the basal level of pro-caspase-3...
  63. ncbi beta-Trcp mediates ubiquitination and degradation of the erythropoietin receptor and controls cell proliferation
    Laure Meyer
    Institut Cochin, Departement d Hematologie, Paris, France
    Blood 109:5215-22. 2007
    ....
  64. pmc Degradation of Mcl-1 by beta-TrCP mediates glycogen synthase kinase 3-induced tumor suppression and chemosensitization
    Qingqing Ding
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Mol Cell Biol 27:4006-17. 2007
    ..Our results indicate that the turnover of Mcl-1 by beta-TrCP is an essential mechanism for GSK-3beta-induced apoptosis and contributes to GSK-3beta-mediated tumor suppression and chemosensitization...
  65. pmc Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum
    Julie Binette
    Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montreal, 110 avenue des Pins Ouest, Montreal, Quebec H2W 1R7, Canada
    Retrovirology 4:75. 2007
    ..Vpu is thought to act as an adaptor protein, connecting CD4 to the ubiquitin (Ub)-proteasome degradative system through an interaction with beta-TrCP, a component of the SCFbeta-TrCP E3 Ub ligase complex...
  66. pmc Site-specific ubiquitination exposes a linear motif to promote interferon-alpha receptor endocytosis
    K G Suresh Kumar
    Department of Animal Biology and 2Mari Lowe Center for Comparative Oncology Research, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 179:935-50. 2007
    ..The SCF(betaTrcp) (Skp1-Cullin1-F-box complex) E3 ubiquitin ligase that mediates IFNAR1 ubiquitination and degradation in cells ..
  67. pmc Control of chromosome stability by the beta-TrCP-REST-Mad2 axis
    Daniele Guardavaccaro
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, New York 10016, USA
    Nature 452:365-9. 2008
    ..The high levels of REST or its truncated variants found in certain human tumours may contribute to cellular transformation by promoting genomic instability...
  68. pmc SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation
    Thomas F Westbrook
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 452:370-4. 2008
    ..Thus, REST is a key target in beta-TRCP-driven transformation and the beta-TRCP-REST axis is a new regulatory pathway controlling neurogenesis...
  69. pmc Plk1- and beta-TrCP-dependent degradation of Bora controls mitotic progression
    Akiko Seki
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 181:65-78. 2008
    ..We conclude that tight regulation of the Bora protein by its synthesis and degradation is critical for cell cycle progression...
  70. doi UV-induced degradation of securin is mediated by SKP1-CUL1-beta TrCP E3 ubiquitin ligase
    M Cristina Limón-Mortés
    Departamento de Microbiologia, Facultad de Biologia, Universidad de Sevilla, Sevilla, Spain
    J Cell Sci 121:1825-31. 2008
    ..Moreover, we show that GSK-3beta inhibitors prevent securin degradation, and that CUL1 and betaTrCP are involved in this depletion...
  71. doi HIV-1 accessory proteins--ensuring viral survival in a hostile environment
    Michael H Malim
    Department of Infectious Diseases, King s College London School of Medicine, 2nd Floor, Borough Wing, Guy s Hospital, London Bridge, London, SE1 9RT, UK
    Cell Host Microbe 3:388-98. 2008
    ..Broadly speaking, the HIV-1 accessory proteins modify the local environment within infected cells to ensure viral persistence, replication, dissemination, and transmission...
  72. pmc Phosphorylation of p53 by IkappaB kinase 2 promotes its degradation by beta-TrCP
    Yifeng Xia
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:2629-34. 2009
    ..Our results identify IKK2 and beta-TrCP1 as novel regulators of the p53 pathway and suggest that blocking of IKK2 and beta-TrCP1 could be a means of regulating p53 stability and thereby modulating its biological activity...
  73. pmc Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis
    Younghoon Kee
    Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 23:555-60. 2009
    ..Nondegradable mutant forms of FANCM retain the FA core complex in the chromatin and disrupt the FA pathway. Our data provide a novel mechanism for the cell cycle-dependent regulation of the FA pathway...
  74. pmc Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  75. pmc Vpu directs the degradation of the human immunodeficiency virus restriction factor BST-2/Tetherin via a {beta}TrCP-dependent mechanism
    Janet L Douglas
    Oregon Health and Science University, Vaccine and Gene Therapy Institute, Beaverton, 97006, USA
    J Virol 83:7931-47. 2009
    ..to act as an adapter linking CD4 with the ubiquitin-proteasome machinery via interaction with the F-box protein betaTrCP. To identify additional cellular betaTrCP-dependent Vpu targets, we performed quantitative proteomics analyses ..
  76. pmc HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation
    Bastien Mangeat
    Department of Dermatology and Venereology, University Hospitals and Medical School of Geneva, University of Geneva, Switzerland
    PLoS Pathog 5:e1000574. 2009
    ..Identification of tetherin binding to Vpu provides a potential novel target for the development of drugs aimed at inhibiting HIV-1 replication...
  77. pmc A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP)
    Bin Zhao
    Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093 0815, USA
    Genes Dev 24:72-85. 2010
    ....
  78. pmc beta-TrCP inhibition reduces prostate cancer cell growth via upregulation of the aryl hydrocarbon receptor
    Udi Gluschnaider
    Department of Pathology and the Lautenberg Center for Immunology, Hebrew University Hadassah Medical School, Jerusalem, Israel
    PLoS ONE 5:e9060. 2010
    ..Thus, alternative strategies targeting novel molecular mechanisms are required. beta-TrCP is an E3 ligase that targets various substrates essential for many aspects of tumorigenesis...
  79. pmc Phosphorylation by casein kinase I promotes the turnover of the Mdm2 oncoprotein via the SCF(beta-TRCP) ubiquitin ligase
    Hiroyuki Inuzuka
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 18:147-59. 2010
    ..Our results provide insight into the signaling pathways controlling Mdm2 destruction and further suggest that compromised regulation of Mdm2 results in attenuated p53 activity, thereby facilitating tumor progression...
  80. pmc Dual-color click beetle luciferase heteroprotein fragment complementation assays
    Victor Villalobos
    Molecular Imaging Center, Mallinckrodt Institute of Radiology, Department of Developmental Biology, Washington University, St Louis, MO 63110, USA
    Chem Biol 17:1018-29. 2010
    ..These dual-color protein interaction switches may enable directed dynamic analysis of a variety of protein interactions in living cells...
  81. pmc Ligand-stimulated downregulation of the alpha interferon receptor: role of protein kinase D2
    Hui Zheng
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104 4539, USA
    Mol Cell Biol 31:710-20. 2011
    ..The mechanisms of the ligand-inducible elimination of IFNAR1 are discussed, along with the potential medical significance of this regulation...
  82. pmc β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release
    Hanna Mari Tervo
    Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany
    Retrovirology 8:9. 2011
    ..Recently, it has been suggested that β-TrCP is also critically involved in Vpu's ability to overcome the CD317-mediated virion release block...
  83. pmc PEST motif serine and tyrosine phosphorylation controls vascular endothelial growth factor receptor 2 stability and downregulation
    Rosana D Meyer
    Department of Pathology, Boston University Medical Campus, 670 Albany St, Boston, MA 02118, USA
    Mol Cell Biol 31:2010-25. 2011
    ..The PEST domain acts as a dual modulator of VEGFR-2; the phosphorylation of S1188/S1191 controls ubiquitination and degradation via β-Trcp1, where the phosphorylation of Y1173 through PKA/p38 MAPK controls the stability of VEGFR-2...
  84. pmc mTOR drives its own activation via SCF(βTrCP)-dependent degradation of the mTOR inhibitor DEPTOR
    DaMing Gao
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Mol Cell 44:290-303. 2011
    ....
  85. pmc DEPTOR, an mTOR inhibitor, is a physiological substrate of SCF(βTrCP) E3 ubiquitin ligase and regulates survival and autophagy
    Yongchao Zhao
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Cell 44:304-16. 2011
    ..Furthermore, DEPTOR accumulates upon glucose deprivation and mTOR inhibition to induce autophagy. Thus, βTrCP-DEPTOR-mTOR intertwine to regulate cell survival and autophagy...
  86. pmc mTOR generates an auto-amplification loop by triggering the βTrCP- and CK1α-dependent degradation of DEPTOR
    Shanshan Duan
    Department of Pathology, NYU Cancer Institute, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 44:317-24. 2011
    ..Moreover, our results suggest that pharmacologic inhibition of CK1 may be a viable therapeutic option for the treatment of cancers characterized by activation of mTOR-signaling pathways...
  87. pmc Fbxw7α- and GSK3-mediated degradation of p100 is a pro-survival mechanism in multiple myeloma
    Luca Busino
    NYU Cancer Institute, New York University School of Medicine, 522 First Avenue, SRB 1107, New York, New York 10016, USA
    Nat Cell Biol 14:375-85. 2012
    ..Thus, in multiple myeloma, Fbxw7α and GSK3 function as pro-survival factors through the control of p100 degradation...
  88. pmc SCF(β-TRCP) suppresses angiogenesis and thyroid cancer cell migration by promoting ubiquitination and destruction of VEGF receptor 2
    Shavali Shaik
    Division of Cancer Biology and Angiogenesis, Department of Pathology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA
    J Exp Med 209:1289-307. 2012
    ..These results provide a new biomarker that may aid a rational use of tyrosine kinase inhibitors to treat refractory PTC...
  89. pmc Human tetherin exerts strong selection pressure on the HIV-1 group N Vpu protein
    Daniel Sauter
    Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany
    PLoS Pathog 8:e1003093. 2012
    ..These results indicate that HIV-1 group N Vpu is under strong host-specific selection pressure and that the acquisition of effective tetherin antagonism may lead to the emergence of viral variants with increased transmission fitness...
  90. pmc SCF-mediated Cdh1 degradation defines a negative feedback system that coordinates cell-cycle progression
    Hidefumi Fukushima
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Cell Rep 4:803-16. 2013
    ....
  91. ncbi Hierarchical order of phosphorylation events commits Cdc25A to betaTrCP-dependent degradation
    Maddalena Donzelli
    European Institute of Oncology, Milan, Italy
    Cell Cycle 3:469-71. 2004
    ..that regulation of Cdc25A protein abundance during S phase and in response to DNA damage is mediated by SCF(betaTrCP) activity...
  92. pmc Nedd8 modification of cul-1 activates SCF(beta(TrCP))-dependent ubiquitination of IkappaBalpha
    M A Read
    LeukoSite, Inc, Cambridge, Massachusetts 02139, USA
    Mol Cell Biol 20:2326-33. 2000
    ..a minute fraction of total cellular Cul-1 is modified by Nedd8, the Cul-1 associated with ectopically expressed betaTrCP was highly enriched for the Nedd8-conjugated form...
  93. ncbi Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase
    Sachie Asada
    Kishi Initiative Research Unit, Frontier Research System, 2 1 Hirosawa, Wako, Saitama, Japan
    Int J Mol Med 22:95-104. 2008
    ..These results suggest that H2O2 induces SCF beta-TrCP-mediated ubiquitination of RCAN1, leading to a decrease in the protein level of RCAN1...
  94. pmc Regulation of postsynaptic RapGAP SPAR by Polo-like kinase 2 and the SCFbeta-TRCP ubiquitin ligase in hippocampal neurons
    Xiaolu L Ang
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:29424-32. 2008
    ..Our results identify a specific E3 ubiquitin ligase that mediates degradation of a key postsynaptic regulator of synaptic morphology and function...
  95. doi Chronic low frequency/low volume resistance training reduces pro-inflammatory cytokine protein levels and TLR4 mRNA in rat skeletal muscle
    Nelo Eidy Zanchi
    Laboratory of Applied Nutrition and Metabolism, Physical Education and Sport School, University of Sao Paulo, Av Prof Mello Moraes, 65, Sao Paulo, SP, 05508 900, Brazil
    Eur J Appl Physiol 109:1095-102. 2010
    ....
  96. pmc Multiple isoforms of beta-TrCP display differential activities in the regulation of Wnt signaling
    Eunjeong Seo
    Department of Life Science, The University of Seoul, Seoul, 130 743, Republic of Korea
    Cell Signal 21:43-51. 2009
    ..Overall, our data suggest that isoforms of beta-TrCPs generated by alternative splicing may have different biological roles...
  97. pmc Analysis of the role of COP9 Signalosome (CSN) subunits in K562; the first link between CSN and autophagy
    Claire Pearce
    College of Life and Environmental Sciences, School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    BMC Cell Biol 10:31. 2009
    ..Although studied in model human cell lines such as HeLa, very little is known about the role of the CSN in haemopoietic cells...
  98. pmc LPS impairs phospholipid synthesis by triggering beta-transducin repeat-containing protein (beta-TrCP)-mediated polyubiquitination and degradation of the surfactant enzyme acyl-CoA:lysophosphatidylcholine acyltransferase I (LPCAT1)
    Chunbin Zou
    Department of Medicine, Acute Lung Injury Center of Excellence, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 286:2719-27. 2011
    ..LPCAT1 is the first lipogenic substrate for β-TrCP, and the results suggest that modulation of the GSK-3β-SCFβ(TrCP) E3 ligase effector pathway might be a unique strategy to optimize dipalmitoylphosphatidylcholine levels in sepsis...
  99. pmc Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome
    Yakir Moshe
    Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
    Proc Natl Acad Sci U S A 101:7937-42. 2004
    ..Transfection with an small interfering RNA duplex directed against Plk1 caused the accumulation of Emi1 in mitotically arrested HeLa cells. It is suggested that phosphorylation of Emi1 by Plk1 is involved in its degradation in mitosis...
  100. ncbi Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor
    K G Suresh Kumar
    Department of Animal Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:46614-20. 2004
    ..We discuss the complexities and specifics of the ubiquitination and degradation of IFNAR1, which is a beta-Trcp substrate that undergoes degradation via a lysosomal pathway...
  101. pmc Disruption of Sag/Rbx2/Roc2 induces radiosensitization by increasing ROS levels and blocking NF-kappaB activation in mouse embryonic stem cells
    Mingjia Tan
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, University of Michigan, Ann Arbor, MI 48109, USA
    Free Radic Biol Med 49:976-83. 2010
    ....

Research Grants36

  1. MOLECULAR MECHANISMS OF THE NEUROSPORA CIRCADIAN CLOCK
    Yi Liu; Fiscal Year: 2013
    ..Because of the similarities between the Neurospora and animal clocks, our results will provide important information on how eukaryotic circadian clock functions. ..
  2. Genetic Analysis of Snail Superfamily Genes in Mice
    THOMAS HOOKER GRIDLEY; Fiscal Year: 2012
    ..mice;2) test the hypothesis that post- translational regulation of SNAI1 protein by the GSK3beta kinase and the betaTrcp ubiquitin ligase are essential for Snai1 function in vivo;3) test the hypothesis that Snail family genes are ..
  3. Wenyi Wei; Fiscal Year: 2015
    ..Our proposed studies will provide new insight into the signaling pathways controlling Mdm2 destruction. It also provides the rationale for developing CKI and ATM agonists as anti-cancer agents. ..
  4. NF-kappaB in myeloma cell growth and survival in vivo
    Babatunde Oyajobi; Fiscal Year: 2009
    ..the finding that expression in myeloma cells, of a dominant-negative (deltaF) mutant of the E3 Ubiquitin ligase betaTrCP/FWD1 that regulates NF-kB signaling, significantly reduced tumor growth in mice in vivo...
  5. SNAIL-Dependent Regulation of EMT in Cancer
    STEPHEN JOSEPH WEISS; Fiscal Year: 2010
    ..These studies should not only define the interlocking role of Wnt, beta-catenin/TCF and Snail in regulating EMT in cancer, but also identify new targets for therapeutic intervention. ..
  6. Stability of prolactin receptor and prolactin signaling
    Serge Y Fuchs; Fiscal Year: 2010
    ..Our preliminary data suggest that (i) the bTrCP E3 ubiquitin ligase limits the extent of PRL signaling via PRLr ubiquitination and lysosomal degradation that ..
  7. JAROSLAW WALDEMAR ZMIJEWSKI; Fiscal Year: 2015
    ..of NF-[unreadable]B, by examining the effects of AMPK activation on IKK-dependent phosphorylation of IkBa, SCF-bTrCP mediated ubiquitination of phosphorylated IkBa and degradation of ubiquitinated IkBa by the 26S proteasome...
  8. Gli Control of PTH-rP and Osteolysis in Breast Cancer
    Gregory Mundy; Fiscal Year: 2009
    ..We propose therefore that the Gli family of transcriptional regulators represents an important molecular pathway that regulates PTH-rP expression in breast cancer cells, and subsequent breast cancer-mediated osteolysis. ..
  9. Vladimir S Spiegelman; Fiscal Year: 2016
    ..CRD-BP is a multifunctional RNA-binding protein that recognizes the mRNAs of MITF, bTrCP1, Gli1, c-myc, IGF2, tau, and other genes...
  10. Regulation of PTHrP expression in chondrocytes by Gli2
    JULIE STERLING; Fiscal Year: 2006
    ..abstract_text> ..
  11. Effect of Gli2 on BMP-2 gene expression & bone formation
    Ming Zhao; Fiscal Year: 2007
    ..Full characterization of the roles of Gli2 protein in the regulation of BMP-2 gene expression and skeletogenesis should lead to insights into the molecular mechanisms of bone anabolic regulation. [unreadable] [unreadable]..
  12. Xom Proteolysis During Early Vertebrate Embryogenesis
    Zhenglun Zhu; Fiscal Year: 2006
    ..abstract_text> ..
  13. Ubiquitination and Degradation in Cancer Therapy
    Kathleen Sakamoto; Fiscal Year: 2005
    ..Development of this technology will lead to novel approaches to treat androgen dependent and -independent disease in hopes of improving the survival of men with prostate cancer. ..
  14. Role of PER phosphorylation in circadian biology
    JOANNA CHIU; Fiscal Year: 2006
    ..I will integrate in vitro assays, tissue culture based systems, and whole animal approaches. The use of Drosophila melanogaster as the model animal provides a powerful system for genetic manipulations. ..
  15. CONSOLE FOR 10.5 TESLA WHOLE BODY MRI SYSTEM
    Kamil Ugurbil; Fiscal Year: 2010
    ..HEI grant will place this advanced instrument in a laboratory that is funded as a Biotechnology Research Center (BTRC) for high field MR research and a laboratory with appropriate interdisciplinary expertise and infrastructure to ..
  16. Ivet Bahar; Fiscal Year: 2016
    DESCRIPTION (provided by applicant): We propose to establish a Biomedical Technology Research Center (BTRC) in High Performance Computing for Multiscale Modeling of Biological Systems, as a joint effort between the U of Pittsburgh (Pitt;..
  17. Carl Fredrik Westin; Fiscal Year: 2016
    The Neuroimage Analysis Center (NAC) is a National Resource Center in the Biomedical Technology Resource Center (BTRC) program that is developing image analysis algorithms and software tools to improve understanding of brain diseases and ..
  18. Vasan Venugopalan; Fiscal Year: 2016
    ..It is the only BTRC with a dedicated technology core devoted to modeling and computation...
  19. Kamil Ugurbil; Fiscal Year: 2016
    ..The central aim of this Biotechnology Research Center (BTRC) grant is to significantly advance such MR based measurement capabilities and their biomedical applications in ..
  20. PEDIATRIC BRAIN TUMOR CLINICAL TRIAL CONSORTIUM
    Michael Prados; Fiscal Year: 2003
    ..Clinical and laboratory investigators in the Brain Tumor Research Center (BTRC) have over 25 years of experience in funded research of the biology and treatment of these diseases...
  21. TREATMENT OF MALIGNANT BRAIN TUMORS
    Mitchel Berger; Fiscal Year: 1999
    ..The Brain Tumor Research Center (BTRC) has developed into a major treatment center for patients with tumors of the brain...
  22. The role of mTOR in Ras mediated gliomagenesis
    JEAN NAKAMURA; Fiscal Year: 2009
    ..Cancer Center, a designated National Cancer Institute research center, and the UCSF Brain Tumor Research Center (BTRC), an NCI named Specialized Program of Research Excellence (SPORE). Mentor Russell O. Pieper, Ph.D...
  23. TRAINING IN BRAIN TUMOR BIOLOGY AND THERAPY
    Mitchel Berger; Fiscal Year: 2001
    ..of two or three years with the first year being spent on the Neuro-Oncology Service in Brain Tumor Research (BTRC). Here they participate in all aspects of treating patients with malignant brain tumors...
  24. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    Gabriel Pineda; Fiscal Year: 2006
    ..The specific ubiquitin ligase of IkappaBalpha has been reported to be SCFBTrCP, which is a multiprotein complex. BTrCP is an F-Box protein that also contains a WD40 repeat motif...
  25. Molecular and Cellular Characterization of Myeloproliferative Disease
    KATHLEEN MIHO SAKAMOTO; Fiscal Year: 2010
    ..These studies will provide new insights into the molecular pathways leading to MPD. ..
  26. Smad4 Stability by beta-TrCP1 in Pancreatic Cancer
    Mei Wan; Fiscal Year: 2005
    ..abstract_text> ..
  27. The cardiac interferon response to reovirus infection
    Barbara Sherry; Fiscal Year: 2008
    ..In sum, results will identify cell type-specific IFN-beta-related responses critical for protection against myocarditis, potentially providing new avenues for intervention against viral infections of the heart. ..
  28. Poxvirus adverse effects on cardiac cells and the heart
    Barbara Sherry; Fiscal Year: 2005
    ..Studies proposed in this R21 application will provide the foundation for future in-depth studies with the goal of improving current and future smallpox vaccines. ..
  29. How Anthrax lethal factor kills activated macrophages
    Michael Karin; Fiscal Year: 2003
    ..anthracis and its host, this project should enable the design of new therapeutic strategies that will tilt the balance in favor of the host macrophage in the battle against B. anthracis and inhalation anthrax. ..
  30. Functions and Mechanisms of Radiation Induced Responses
    Michael Karin; Fiscal Year: 2006
    ..abstract_text> ..
  31. Genetics of Cell Cycle Regulators in C. elegans
    Edward T Kipreos; Fiscal Year: 2010
    ..The abstract has been modified to eliminate the previously proposed screen to identify cullin PTMs. ..
  32. INTERACTIONS OF HEAVY METAL IONS WITH THE HUMAN GENOME
    Michael Karin; Fiscal Year: 2007
    ..As NF-KB plays a key role in inflammation, understanding the regulation of IKK activity by oxidants, such as ozone, will provide a molecular basis for oxidant induced inflammatory disease. ..
  33. ACUTE MYOCARDITIS--ROLES OF REOVIRUS AND INTERFERON BETA
    Barbara Sherry; Fiscal Year: 2001
    ..Reoviruses offer a unique tool to investigate the IFN response invoked by closely related viruses in the heart. Our work will be the first to identity cell factors that are determinants of this cardiac IFN response and myocarditis. ..
  34. Molecular Mechanisms in Vertebrate Midbrain Development
    Seema Agarwala; Fiscal Year: 2010
    ..Understanding the specification of midbrain neurons is also crucial to the development of stem cell based therapeutic strategies to combat Parkinson's disease and stroke. ..
  35. Establishment of C.elegans tissue culture cell lines
    EDWARD KIPREOS; Fiscal Year: 2007
    ..The availability of cell lines would greatly extend research using this organism to provide further health-related insights. [unreadable] [unreadable]..
  36. How Anthrax lethal factor kills macrophages
    Michael Karin; Fiscal Year: 2008
    ..Interference with this activation process may be used to increase host resistance to bacterial infections, both with agents of bioterrorism and more common pathogens. ..