Brn 3b

Summary

Gene Symbol: Brn 3b
Description: POU class 4 homeobox 2
Alias: BRN3.2, BRN3B, Brn-3b, POU domain, class 4, transcription factor 2, Brn3b POU domain transcription factor, POU domain protein, brain-3B, brain-specific homeobox/POU domain protein 3B
Species: human

Top Publications

  1. ncbi Brn3a-expressing retinal ganglion cells project specifically to thalamocortical and collicular visual pathways
    Lely A Quina
    Department of Psychiatry, University of California, San Diego, Veterans Affairs San Diego Healthcare System, La Jolla, California 92093 0603, USA
    J Neurosci 25:11595-604. 2005
  2. ncbi The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons
    M Xiang
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 15:4762-85. 1995
  3. ncbi Brn-3b: a POU domain gene expressed in a subset of retinal ganglion cells
    M Xiang
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Neuron 11:689-701. 1993
  4. ncbi Inhibition of neuronal process outgrowth and neuronal specific gene activation by the Brn-3b transcription factor
    M D Smith
    Department of Molecular Pathology, University College London Medical School, United Kingdom
    J Biol Chem 272:1382-8. 1997
  5. pmc POU transcription factors Brn-3a and Brn-3b interact with the estrogen receptor and differentially regulate transcriptional activity via an estrogen response element
    V Budhram-Mahadeo
    Department of Molecular Pathology, The Windeyer Institute of Medical Sciences, University College Medical School, London, United Kingdom
    Mol Cell Biol 18:1029-41. 1998
  6. ncbi The Brn-3b POU family transcription factor regulates the cellular growth, proliferation, and anchorage dependence of MCF7 human breast cancer cells
    J H Dennis
    Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Oncogene 20:4961-71. 2001
  7. ncbi Activation of CDK4 gene expression in human breast cancer cells by the Brn-3b POU family transcription factor
    Laila Samady
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London, UK
    Cancer Biol Ther 3:317-23. 2004
  8. ncbi The Brn-3b transcription factor regulates the growth, behavior, and invasiveness of human neuroblastoma cells in vitro and in vivo
    Shazia Irshad
    Medical Molecular Biology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 12EH, UK
    J Biol Chem 279:21617-27. 2004
  9. ncbi Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter
    Sonia A Lee
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Cancer Res 65:3072-80. 2005
  10. ncbi The Brn-3b POU family transcription factor represses plakoglobin gene expression in human breast cancer cells
    Laila Samady
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London, United Kingdom
    Int J Cancer 118:869-78. 2006

Research Grants

  1. Xiuqian Mu; Fiscal Year: 2015
  2. Deborah C Otteson; Fiscal Year: 2015
  3. CELLULAR AND MOLECULAR MECHANISMS OF GLAUCOMA IN AN ANIMAL MODEL
    Richard Lee; Fiscal Year: 2009
  4. Transcriptional Regulation of Retinal Development
    Mengqing Xiang; Fiscal Year: 2012
  5. IDENTIFICATION OF GENES REGULATING RETINAL GANGLION CELL
    WILLIAM KLEIN; Fiscal Year: 2002
  6. GROWTH FACTORS IN MAMMALIAN EYE DEVELOPMENT
    Jeffrey Bennett; Fiscal Year: 2002
  7. Math5 Regulatory Pathway in Retinal Development
    Lin Gan; Fiscal Year: 2010
  8. EXPRESSION OF SNRNA GENES
    Arne Stenlund; Fiscal Year: 2006
  9. Selective Silencing of Mammalian Neurons: Proof of Mice
    Henry Lester; Fiscal Year: 2004
  10. Brn-3 Transcription Factors in Retinal Development
    Lin Gan; Fiscal Year: 2007

Scientific Experts

Detail Information

Publications144 found, 100 shown here

  1. ncbi Brn3a-expressing retinal ganglion cells project specifically to thalamocortical and collicular visual pathways
    Lely A Quina
    Department of Psychiatry, University of California, San Diego, Veterans Affairs San Diego Healthcare System, La Jolla, California 92093 0603, USA
    J Neurosci 25:11595-604. 2005
    ..Brn3a is coexpressed extensively with the closely related factor Brn3b in the embryonic retina, and the effects of the loss of Brn3a in retinal development are not severe, suggesting ..
  2. ncbi The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ganglion cells and somatosensory neurons
    M Xiang
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 15:4762-85. 1995
    ....
  3. ncbi Brn-3b: a POU domain gene expressed in a subset of retinal ganglion cells
    M Xiang
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Neuron 11:689-701. 1993
    ..POU domain transcription factors in human retina cDNA has led to the identification of Brn-3b, a class IV POU domain protein. Immunohistochemical experiments show that chicken, mouse, rabbit, monkey, and human retinas contain Brn-3b ..
  4. ncbi Inhibition of neuronal process outgrowth and neuronal specific gene activation by the Brn-3b transcription factor
    M D Smith
    Department of Molecular Pathology, University College London Medical School, United Kingdom
    J Biol Chem 272:1382-8. 1997
    ..Hence a single amino acid difference results in these closely related factors having opposite effects and allows the balance between them to regulate differentiation...
  5. pmc POU transcription factors Brn-3a and Brn-3b interact with the estrogen receptor and differentially regulate transcriptional activity via an estrogen response element
    V Budhram-Mahadeo
    Department of Molecular Pathology, The Windeyer Institute of Medical Sciences, University College Medical School, London, United Kingdom
    Mol Cell Biol 18:1029-41. 1998
    ..These observations and their implications for transcriptional regulation by the ER are discussed...
  6. ncbi The Brn-3b POU family transcription factor regulates the cellular growth, proliferation, and anchorage dependence of MCF7 human breast cancer cells
    J H Dennis
    Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
    Oncogene 20:4961-71. 2001
    ..These results thus further indicate a role for the Brn-3b transcription factor in regulating mammary cell growth and suggest that its elevation in breast cancer is of functional significance...
  7. ncbi Activation of CDK4 gene expression in human breast cancer cells by the Brn-3b POU family transcription factor
    Laila Samady
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London, UK
    Cancer Biol Ther 3:317-23. 2004
    ..The effect of Brn-3b on the growth regulatory CDK4 protein provides a further mechanism by which Brn-3b can regulate breast cancer cell growth and indicates that it can do this by activating as well as repressing specific target genes...
  8. ncbi The Brn-3b transcription factor regulates the growth, behavior, and invasiveness of human neuroblastoma cells in vitro and in vivo
    Shazia Irshad
    Medical Molecular Biology Unit, Institute of Child Health, 30 Guilford Street, London WC1N 12EH, UK
    J Biol Chem 279:21617-27. 2004
    ..It is possible that decreasing Brn-3b levels may reverse some effects on growth and proliferation of these cells...
  9. ncbi Expression of the Brn-3b transcription factor correlates with expression of HSP-27 in breast cancer biopsies and is required for maximal activation of the HSP-27 promoter
    Sonia A Lee
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Cancer Res 65:3072-80. 2005
    ..Thus, Brn-3b can, directly and indirectly (via interaction with the ER), activate HSP-27 expression, and this may represent one mechanism by which Brn-3b mediates its effects in breast cancer cells...
  10. ncbi The Brn-3b POU family transcription factor represses plakoglobin gene expression in human breast cancer cells
    Laila Samady
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London, United Kingdom
    Int J Cancer 118:869-78. 2006
    ....
  11. ncbi Proliferation-associated Brn-3b transcription factor can activate cyclin D1 expression in neuroblastoma and breast cancer cells
    V S Budhram-Mahadeo
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London, UK
    Oncogene 27:145-54. 2008
    ..Site-directed mutagenesis of this sequence resulted in loss of transactivation of the CD1 promoter by Brn-3b. Thus, Brn-3b may act to alter growth properties of breast cancer and NB cells by enhancing CD1 expression in these cells...
  12. ncbi Delayed expression of the Crx gene and photoreceptor development in the Chx10-deficient retina
    Adam D Rutherford
    Developmental Biology Unit, Institute of Child Health, University College London, London, United Kingdom
    Invest Ophthalmol Vis Sci 45:375-84. 2004
    ..The purpose of this study was to examine how neuronal development is affected by absence of the Chx10 transcription factor in the mouse retina...
  13. ncbi Identification of chaperonin CCT gamma subunit as a determinant of retinotectal development by whole-genome subtraction cloning from zebrafish no tectal neuron mutant
    Naoto Matsuda
    Department of Molecular Neurobiology and Pharmacology, Graduate School of Medicine, University of Tokyo, and SORST, Japan Science and Technology Agency, Tokyo 113 0033, Japan
    Development 131:1913-25. 2004
    ..The expression patterns of transcription factor genes ath5 and brn3b that are essential for the development and maintenance of RGCs were indistinguishable between wild-type and ntn ..
  14. pmc Laser-induced ocular hypertension in albino CD-1 mice
    Christine T Fu
    Neuroscience Graduate Program, Department of Ophthalmology, University of California, San Francisco, San Francisco, California 94143, USA
    Invest Ophthalmol Vis Sci 51:980-90. 2010
    ..To establish a laser-induced model of ocular hypertension (LIOH) in albino CD-1 mice and to characterize the sequence of pathologic events triggered by intraocular pressure (IOP) elevation...
  15. ncbi Microarray analysis reveals differential gene expression patterns in tumors of the pineal region
    Michelle Fevre-Montange
    University Claude Bernard Lyon 1, Hopital Neurologique, BP Lyon Montchat, Lyon, France
    J Neuropathol Exp Neurol 65:675-84. 2006
    ..Our results highlight the usefulness of gene expression profiling for classify tumors of the pineal region and identify genes with potential use as diagnostic markers...
  16. ncbi Quantitative ex vivo detection of rodent retinal ganglion cells by immunolabeling Brn-3b
    Kathleen M Leahy
    Pfizer Corporation, St Louis, MO, USA
    Exp Eye Res 79:131-40. 2004
    ..Thus, ex vivo immunolabeling of retinal ganglion cells using antibody to Brn-3b provides an alternative to other methods of quantifying retinal ganglion cells...
  17. pmc The class III POU domain protein Brn-1 can fully replace the related Oct-6 during schwann cell development and myelination
    Ralf P Friedrich
    Institut fur Biochemie, Universitat Erlangen, Fahrstrasse 17, 91054 Erlangen, Germany
    Mol Cell Biol 25:1821-9. 2005
    ..Although the closely related POU domain protein Brn-2 is coexpressed with Oct-6 in Schwann cells, its loss has only mild consequences...
  18. pmc Differential splicing of the GHF1 primary transcript gives rise to two functionally distinct homeodomain proteins
    L E Theill
    Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093 0636
    EMBO J 11:2261-9. 1992
    The POU domain protein GHF-1 has a critical role in generation, proliferation and phenotypic expression of three pituitary cell types...
  19. ncbi Identification of the nuclear localization signal of the POU domain protein Tst-1/Oct6
    E Sock
    Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Martinistrasse 52, D 20246 Hamburg, Federal Republic of Germany
    J Biol Chem 271:17512-8. 1996
    ....
  20. pmc Essential role of POU-domain factor Brn-3c in auditory and vestibular hair cell development
    M Xiang
    Center for Advanced Biotechnology and Medicine, Department of Pediatrics, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 94:9445-50. 1997
    ....
  21. pmc Luman, a new member of the CREB/ATF family, binds to herpes simplex virus VP16-associated host cellular factor
    R Lu
    Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada
    Mol Cell Biol 17:5117-26. 1997
    ..function is to stabilize the herpes simplex virus virion transactivator VP16 in a complex with the cellular POU domain protein Oct-1 and cis-acting regulatory elements in promoters of immediate-early viral genes...
  22. pmc Brn3a is a transcriptional regulator of soma size, target field innervation and axon pathfinding of inner ear sensory neurons
    E J Huang
    Program in Neuroscience, Department of Physiology, and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Development 128:2421-32. 2001
    The POU domain transcription factors Brn3a, Brn3b and Brn3c are required for the proper development of sensory ganglia, retinal ganglion cells, and inner ear hair cells, respectively...
  23. pmc Interaction of HCF-1 with a cellular nuclear export factor
    Shahana S Mahajan
    Department of Microbiology and the Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    J Biol Chem 277:44292-9. 2002
    ..and, after release into the cell, binds to HCF-1 and translocates to the nucleus to form a complex with the POU domain protein Oct-1 and a VP16-responsive DNA sequence...
  24. pmc Subtype specification of GABAergic amacrine cells by the orphan nuclear receptor Nr4a2/Nurr1
    Haisong Jiang
    Center for Advanced Biotechnology and Medicine, Department of Pediatrics, and Graduate Program in Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    J Neurosci 29:10449-59. 2009
    ..Moreover, the expression of Nr4a2 is positively regulated by Foxn4 and negatively controlled by Brn3b, two retinogenic factors previously shown to promote and suppress GABAergic ACs, respectively...
  25. ncbi Localized expression of a Xenopus POU gene depends on cell-autonomous transcriptional activation and induction-dependent inactivation
    D Frank
    Department of Molecular and Cellular Biology, University of California, Berkeley 94720
    Development 115:439-48. 1992
    We have cloned a cDNA encoding a Xenopus POU domain protein, XLPOU91, which is expressed at high levels in gastrula embryos. XLPOU91 transcription initiates at the midblastula transition, and declines to low levels by late neurula stages...
  26. pmc A novel complex regulates cardiac actin gene expression through interaction of Emb, a class VI POU domain protein, MEF2D, and the histone transacetylase p300
    S Molinari
    CNRS URA 2578, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France
    Mol Cell Biol 24:2944-57. 2004
    ..Oct1 and Emb, a class VI POU domain protein, bind to consensus sites on the DNA, and it is the binding of Emb which is important for activity...
  27. ncbi The solution structure of the Oct-1 POU-specific domain reveals a striking similarity to the bacteriophage lambda repressor DNA-binding domain
    N Assa-Munt
    Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037
    Cell 73:193-205. 1993
    ..A human dwarfism mutation that affects positive control in the related POU domain protein Pit-1 maps to the same region of the HTH motif as do positive control mutations in lambda repressor.
  28. doi Conserved role of the Vsx genes supports a monophyletic origin for bilaterian visual systems
    Ted Erclik
    Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, TMDT Building, 101 College Street, Toronto, Ontario M5G 1L7, Canada
    Curr Biol 18:1278-87. 2008
    ....
  29. ncbi Binding of a Drosophila POU-domain protein to a sequence element regulating gene expression in specific dopaminergic neurons
    W A Johnson
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Nature 343:467-70. 1990
    ..The Cfla gene product binds to a DNA element required for expression of the dopa decarboxylase gene (Ddc) in selected dopaminergic neurons, implying that it functions as a neuron-specific transcription factor...
  30. ncbi Identification of an N-terminal transcriptional activation domain within Brn3b/POU4f2
    Suzanna E Martin
    Department of Biochemistry and Molecular Biology, Unit 117, The University of Texas, M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Differentiation 73:18-27. 2005
    The POU-domain transcription factor Brn3b/ POU4f2 is an essential regulator of gene expression in mouse retinal ganglion cells...
  31. pmc Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression
    Vishwanie S Budhram-Mahadeo
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Nucleic Acids Res 34:6640-52. 2006
    ..Thus, the ability of Brn-3b to interact with p53 and modulate Bax expression may demonstrate an important mechanism that helps to determine the fate of cells when p53 is induced...
  32. pmc A novel POU domain protein which binds to the T-cell receptor beta enhancer
    H Messier
    Division of Molecular Biology, La Jolla Institute for Allergy and Immunology, California 92037
    Mol Cell Biol 13:5450-60. 1993
    ..It was therefore of special interest when we identified a novel lymphoid POU domain protein in Southwestern (DNA-protein) screens of T-cell lambda gt11 libraries...
  33. ncbi NT-3 regulates expression of Brn3a but not Brn3b in developing mouse trigeminal sensory neurons
    S Wyatt
    Department of Molecular Pathology, University College London Medical School, The Windeyer Building, 46 Cleveland Street, London, WIP 6DB, UK
    Brain Res Mol Brain Res 55:254-64. 1998
    We have used a quantitative RT-PCR approach to determine the levels of Brn3a and Brn3b POU domain transcription factor mRNAs in the developing mouse trigeminal ganglion from E10 to E18...
  34. ncbi Cooperative dimerization of the POU domain protein Brn-2 on a new motif activates the neuronal promoter of the human aromatic L-amino acid decarboxylase gene
    Claire Dugast-Darzacq
    Laboratoire de Biologie Moleculaire Eucaryote, CNRS UMR 5099 IFR 109, 118 route de Narbonne, 31062, Cedex, Toulouse, France
    Brain Res Mol Brain Res 120:151-63. 2004
    ..decarboxylase (AADC) gene contains a perfectly palindromic element (TB) that conforms to the structure of a POU domain protein binding site of the MORE+2 type...
  35. pmc Eomesodermin, a target gene of Pou4f2, is required for retinal ganglion cell and optic nerve development in the mouse
    Chai An Mao
    Department of Biochemistry and Molecular Biology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Development 135:271-80. 2008
    ..We conclude that Eomes is a crucial regulator positioned immediately downstream of Pou4f2 and is required for RGC differentiation and optic nerve development...
  36. pmc Gene regulation logic in retinal ganglion cell development: Isl1 defines a critical branch distinct from but overlapping with Pou4f2
    Xiuqian Mu
    Department of Biochemistry and Molecular Biology, M D Anderson Cancer Center and Graduate Training Program in Genes and Development, Graduate School of Biomedical Sciences, University of Texas, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:6942-7. 2008
    ..They also reveal that identical RGC expression patterns are achieved by different combinations of divergent inputs from upstream transcription factors...
  37. doi Cell-specific regulation of the pro-survival Brn-3b transcription factor by microRNAs
    Mattia Calissano
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, WC1N 1EH London, UK
    Mol Cell Neurosci 45:317-23. 2010
    ..We speculate that this mechanism could have a widespread role in the regulation of mRNAs encoding for essential proteins...
  38. pmc The Brn-3a transcription factor induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins
    M D Smith
    Department of Molecular Pathology, University College London Medical School, United Kingdom
    Mol Cell Biol 17:345-54. 1997
    ..Thus, Brn-3a appears to play a critical role in the specification of the mature neuronal phenotype, acting by stimulating the expression of genes whose products are required for process outgrowth and synapse formation...
  39. ncbi I-POU: a POU-domain protein that inhibits neuron-specific gene activation
    M N Treacy
    Eukaryotic Regulatory Biology Program, University of California, San Diego School of Medicine, La Jolla 92093 0648
    Nature 350:577-84. 1991
    ..The inhibition by I-POU provides a potential strategy by which the activation of genes in development is controlled by a homeodomain-containing protein that does not bind DNA...
  40. ncbi The unc-86 gene product couples cell lineage and cell identity in C. elegans
    M Finney
    Department of Genetics, Harvard Medical School, Boston, Massachusetts
    Cell 63:895-905. 1990
    ..Consistent with the fact that unc-86 encodes a POU domain protein, we find that the unc-86 protein is localized to the nucleus...
  41. ncbi Nubbin encodes a POU-domain protein required for proximal-distal patterning in the Drosophila wing
    M Ng
    Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Development 121:589-99. 1995
    ....
  42. ncbi Nonpituitary human prolactin gene transcription is independent of Pit-1 and differentially controlled in lymphocytes and in endometrial stroma
    B Gellersen
    Institute for Hormone and Fertility Research, University of Hamburg, Germany
    Mol Endocrinol 8:356-73. 1994
    ..We focused our studies on the role of Pit-1 and of PR as potential transcriptional regulators, since the POU domain protein Pit-1 is essential in the control of pituitary PRL expression, and progesterone induces decidual ..
  43. ncbi Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes
    T Theil
    Institut für Molekularbiologie und Tumorforschung IMT, Philipps Universitat Marburg, Germany
    Cytogenet Cell Genet 66:267-71. 1994
    ..Fluorescence in situ hybridization experiments with mouse chromosomes showed that Brn-3a maps to mouse chromosome 14E1-3, Brn-3b to XF1-5 and Brn-3c to 18B3-E1...
  44. pmc Cloning, chromosomal localization and expression pattern of the POU domain gene Oct-11
    A S Goldsborough
    Chester Beatty Laboratories, Institute of Cancer Research, London, UK
    Nucleic Acids Res 21:127-34. 1993
    ..We have cloned and sequenced cDNAs encoding a novel mouse POU domain protein, Oct-11, that is closely related within the POU domain to the POU class II proteins, Oct-1 and Oct-2...
  45. ncbi Binding preferences of the POU domain protein Brain-4: implications for autoregulation
    K F Malik
    Laboratory of Cell Biology, NIMH, NIH, Bethesda, MD 20892, USA
    Brain Res Mol Brain Res 38:209-21. 1996
    ..Furthermore, Brn-4 can transactivate from this region of the Brn-4 promoter, suggesting that Brn-4 expression may be autoregulated...
  46. pmc The POU-domain factor Brn-3.0 recognizes characteristic sites in the herpes simplex virus genome
    E E Turner
    Department of Psychiatry, University of California, San Diego, La Jolla, CA 92093 0603, USA
    Nucleic Acids Res 25:2589-94. 1997
    ..The experiments described also provide an effective general method for exhaustive screening of large viral genomes or sub-genomic fragments of eukaryotic DNA for sites of interaction with specific transcription factors...
  47. ncbi Selective inhibition of prolactin gene transcription by the ETS-2 repressor factor
    R N Day
    Departments of Internal Medicine and Cell Biology, National Science Foundation Center for Biological Timing, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 273:31909-15. 1998
    Regulation of prolactin gene transcription requires cooperative interactions between the pituitary-specific POU domain protein Pit-1 and members of the ETS transcription factor family...
  48. ncbi Interaction between the bHLH-PAS protein Trachealess and the POU-domain protein Drifter, specifies tracheal cell fates
    E Zelzer
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Mech Dev 91:163-73. 2000
    ..A direct interaction between Drifter and Trh proteins, mediated by the PAS domain of Trh and the POU domain of Drifter, was demonstrated...
  49. ncbi The closely related POU family transcription factors Brn-3a and Brn-3b are expressed in distinct cell types in the testis
    V Budhram-Mahadeo
    Medical Molecular Biology Unit, Institute of Child Health, University College London, 30 Guilford Street, WC1N 1EH, London, UK
    Int J Biochem Cell Biol 33:1027-39. 2001
    ..Hence, as observed in neuronal cells, Brn-3a and Brn-3b may play distinct and important functional roles in the regulation of gene expression during germ cell development...
  50. ncbi Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth
    Steven W Wang
    Department of Biochemistry and Molecular Biology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Development 129:467-77. 2002
    ..During retinal ganglion cell differentiation, Brn3b is expressed first, followed by Brn3a and Brn3c...
  51. ncbi Targeting Brn-3b in breast cancer therapy
    Vishwanie S Budhram-Mahadeo
    Medical Molecular Biology Unit, Institute of Child Health, University College London, London WC1N 1EH, UK
    Expert Opin Ther Targets 10:15-25. 2006
    ..This review discusses the effect of altering Brn-3b in these cancer cells and possible approaches to targeting Brn-3b as a strategy for therapy in treatment of breast cancers...
  52. pmc ISL1 and BRN3B co-regulate the differentiation of murine retinal ganglion cells
    Ling Pan
    Department of Ophthalmology, University of Rochester, Rochester, NY 14642, USA
    Development 135:1981-90. 2008
    ..Here, we show that ISL1, a LIM-HD protein, is co-expressed with BRN3B, a POU-HD factor, in nascent post-mitotic retinal ganglion cells (RGCs)...
  53. pmc Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina
    Iona D Raymond
    Department of Neurobiology, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, CA 90095 1763, USA
    Mol Vis 14:1559-74. 2008
    ..To characterize cyan fluorescent protein (CFP) expression in the retina of the thy1-CFP (B6.Cg-Tg(Thy1-CFP)23Jrs/J) transgenic mouse line...
  54. pmc Retinal ganglion cell death and optic nerve degeneration by genetic ablation in adult mice
    Jang Hyeon Cho
    Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Exp Eye Res 88:542-52. 2009
    ..To achieve this, we took advantage of Pou4f2 (Brn3b), a gene activated immediately as RGCs begin to differentiate and expressed throughout life...
  55. pmc Overlapping spatiotemporal patterns of regulatory gene expression are required for neuronal progenitors to specify retinal ganglion cell fate
    Takae Kiyama
    Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Vision Res 51:251-9. 2011
    ..We conclude that RPCs have a complex regulatory gene expression program in which they acquire competence using highly integrated mechanisms...
  56. doi Brn-3b inhibits generation of amacrine cells by binding to and negatively regulating DLX1/2 in developing retina
    L Feng
    Department of Ophthalmology, University of Rochester, NY 14642, USA
    Neuroscience 195:9-20. 2011
    ..Thus, DLX1/2 play an essential role in cell fate selection between amacrine and RGCs. Brn-3b suppresses the role of DLX1/2 through physical interaction and biases the competent precursors toward RGC fates...
  57. pmc RIT2, a neuron-specific small guanosine triphosphatase, is expressed in retinal neuronal cells and its promoter is modulated by the POU4 transcription factors
    Ling Zhang
    The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Mol Vis 19:1371-86. 2013
    ..RIT2 is a unique member of the Ras family in that RIT2 is preferentially expressed in various neurons, including retinal neurons. The mechanisms that regulate RIT2 expression in neurons were studied...
  58. pmc Chemically induced specification of retinal ganglion cells from human embryonic and induced pluripotent stem cells
    Hamidreza Riazifar
    Department of Pediatrics, Division of Human Genetics, Department of Anatomy and Neurobiology, Department of Psychiatry and Human Behavior, MitoMed Molecular Diagnostic Laboratory, Department of Pathology, Department of Developmental and Cell Biology, and Department of Ophthalmology, University of California, Irvine, Irvine, California, USA Division of Human Genetics, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio, USA
    Stem Cells Transl Med 3:424-32. 2014
    ..The expression of neural and RGC markers (BRN3A, BRN3B, ATOH7/Math5, γ-synuclein, Islet-1, and THY-1) was examined...
  59. doi Repetitive mild traumatic brain injury causes optic nerve and retinal damage in a mouse model
    Radouil Tzekov
    From the Roskamp Institute, Sarasota RT, AQ, MG, DB, CF, BM, MM, FC and University of South Florida, Tampa RT, Florida Ophthy DS, Inc, Kalamazoo, Michigan JJ and James A Haley Veteran s Administration, Tampa, Florida RT, BM, MM, FC
    J Neuropathol Exp Neurol 73:345-61. 2014
    ..cellularity in the retinal ganglion cell layer and approximately 67% decrease in brain-specific homeobox/POU domain protein 3A-positive retinal ganglion cells in retinal flat mounts...
  60. doi Induction of the ganglion cell differentiation program in human retinal progenitors before cell cycle exit
    Marek Pacal
    Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto Department of Ophthalmology and Vision Sciences, Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
    Dev Dyn 243:C1. 2014
    ..Results: Induction of POU4F2 (BRN3B) marks ganglion cell commitment, and we detected this factor in S-phase progenitors that had already silenced ..
  61. pmc Transcriptome of Atoh7 retinal progenitor cells identifies new Atoh7-dependent regulatory genes for retinal ganglion cell formation
    Zhiguang Gao
    Department of Biochemistry and Molecular Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas, 77030
    Dev Neurobiol 74:1123-40. 2014
    ..Our results expand current knowledge of the regulatory networks operating in Atoh7-expressing RPCs and offer new directions for exploring the earliest aspects of retinogenesis...
  62. doi Functional analysis of the nuclear localization signal of the POU transcription factor Skn‑1a in epidermal keratinocytes
    Ryuta Moritsugu
    Department of Dermatology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036 8562, Japan
    Int J Mol Med 34:539-44. 2014
    ..Skn‑1a, a member of the POU domain protein family, appears to be expressed predominantly in epidermal keratinocytes and is thought to play a critical ..
  63. doi Class III/IV POU transcription factors expressed in small cell lung cancer cells are involved in proneural/neuroendocrine differentiation
    Jun Ishii
    Department of Pathology, Kyorin University School of Medicine, Mitaka, Japan
    Pathol Int 64:415-22. 2014
    ..These findings suggest that the expression of class III/IV POU genes is important for the proneural/neuroendocrine differentiation of lung cancer cells. ..
  64. doi The transcriptional regulator BCL6 participates in the secondary gene regulatory response to vitamin D
    Veijo Nurminen
    School of Medicine, Institute of Biomedicine, University of Eastern Finland, FI 70211 Kuopio, Finland
    Biochim Biophys Acta 1849:300-8. 2015
    ..In conclusion, our study indicated that in monocytes most of the physiological responses to 1,25(OH)2D3 involve the action of the transcription factor BCL6...
  65. doi Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network
    Reza Roozafzoon
    Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
    Biochem Biophys Res Commun 457:154-60. 2015
    ..Immuncytochemical and gene expression analysis revealed the expression of Pax6, Atoh7 and BRN3B increased in 3D fibrin culture compared to 2D conventional culture...
  66. pmc Profound hyperglycemia in knockout mutant mice identifies novel function for POU4F2/Brn-3b in regulating metabolic processes
    Stavroula Bitsi
    Medical Molecular Biology Unit, University College London Institute of Child Health, London, United Kingdom
    Am J Physiol Endocrinol Metab 310:E303-12. 2016
    ....
  67. pmc An epigenetic biomarker combination of PCDH17 and POU4F2 detects bladder cancer accurately by methylation analyses of urine sediment DNA in Han Chinese
    Yongqiang Wang
    Department of Urology, Sun Yat sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Guangzhou, 510060, China
    Oncotarget 7:2754-64. 2016
    ..The combination of POU4F2/PCDH17 has yielded the highest sensitivity and specificity of 90.00% and 93.96% in all the 312 individuals, showing the capability of detecting BlCa effectively among pathologically varied sample groups. ..
  68. pmc Molecular features distinguish ten neuronal types in the mouse superficial superior colliculus
    Haewon Byun
    Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut, 06511
    J Comp Neurol 524:2300-21. 2016
    ..3, netrin G2, cadherin 6, protocadherin 20, retinoid-related orphan receptor β, brain-specific homeobox/POU domain protein 3b, Ets variant gene 1, substance P, somatostatin, vasoactive intestinal polypeptide, and parvalbumin...
  69. pmc Substituting mouse transcription factor Pou4f2 with a sea urchin orthologue restores retinal ganglion cell development
    Chai An Mao
    Department of Systems Biology, Unit 0950, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Proc Biol Sci 283:20152978. 2016
    ....
  70. pmc Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas
    Sumana R Chintalapudi
    Department of Ophthalmology, The University of Tennessee Health Science Center Memphis, TN, USA
    Front Aging Neurosci 8:93. 2016
    ..labeling of homogeneous sorted cells for the intracellular RGC-markers SNCG, brain-specific homeobox/POU domain protein 3A (BRN3A), TUJ1, and RNA-binding protein with multiple splicing (RBPMS); and (3) by applying the ..
  71. pmc Novel Roles and Mechanism for Krüppel-like Factor 16 (KLF16) Regulation of Neurite Outgrowth and Ephrin Receptor A5 (EphA5) Expression in Retinal Ganglion Cells
    Jianbo Wang
    From the Departments of Physiological Optics and Vision Science and
    J Biol Chem 291:18084-95. 2016
    ....
  72. ncbi Genetic labelling of specific axonal pathways in the mouse central nervous system
    Mohamad Zubair
    Division of Molecular Neurobiology, National Institute for Basic Biology, Myodaiji cho, Okazaki 444 8585, Japan
    Eur J Neurosci 15:807-14. 2002
    ..The reporter gene was introduced into the genome under the control of a promoter element of Brn3b transcription factor to establish transgenic mouse lines...
  73. ncbi Coupling the transcriptional regulatory action of Brn-3b to the cell cycle clock
    Tao Zhu
    Breast Cancer Program, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Biol Ther 3:324-5. 2004
  74. ncbi [Studies on the effects of lead on the growth and differentiation of hippocampal neural cells as well as the expression of Oct-2]
    Jun Chen
    Department of Occupational and Environmental Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhonghua Yu Fang Yi Xue Za Zhi 38:151-4. 2004
    In order to explore the effects of lead on the growth and development of cultured hippocampal neural cells and on the expression of Oct-2, the II subtype POU domain protein.
  75. ncbi Elevated expression of the Brn-3a and Brn-3b transcription factors in systemic lupus erythematosus correlates with antibodies to Brn-3 and overexpression of Hsp90
    B J M Ripley
    University College London, London, UK
    Arthritis Rheum 52:1171-9. 2005
    ....
  76. ncbi Expression of AmphiPOU-IV in the developing neural tube and epidermal sensory neural precursors in amphioxus supports a conserved role of class IV POU genes in the sensory cells development
    Simona Candiani
    Department of Biology, University of Genoa, Viale Benedetto XV, 5, Genoa, 16132, Italy
    Dev Genes Evol 216:623-33. 2006
    ....
  77. ncbi Maternal control of vertebrate dorsoventral axis formation and epiboly by the POU domain protein Spg/Pou2/Oct4
    Gerlinde Reim
    Biotechnology Center and Center for Regenerative Therapies, University of Technology TU Dresden, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Development 133:2757-70. 2006
    ..Based on the maternal requirement for pou2 in ventral specification, we propose that ventral specification employs an active, pou2-dependent maternal induction step, rather than a default ventralizing program...
  78. pmc Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line
    Elena Ivanova
    Department of Ophthalmology and Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    J Comp Neurol 521:2165-80. 2013
    ..The remaining two types were identified as putative On-Off and On-DSGCs. This Cre mouse line could be useful for further studies of the molecular and functional properties of BGCs in mice...
  79. ncbi The Wilms' tumor suppressor Wt1 encodes a transcriptional activator of the class IV POU-domain factor Pou4f2 (Brn-3b)
    Kay Dietrich Wagner
    Johannes Müller Institut für Physiologie, Humboldt Universitat, Charite, Tucholskystrasse 2, 10117 Berlin, Germany
    Gene 305:217-23. 2003
    ..Co-localization of both proteins in glomerular podocytes of the kidney and in developing retinal ganglion cells suggests a role for Wt1-Pou4f2 interaction in these tissues...
  80. doi Establishment of retinal progenitor cell clones by transfection with Pax6 gene of mouse induced pluripotent stem (iPS) cells
    Noboru Suzuki
    Department of Immunology, St Marianna University School of Medicine, Kawasaki, Japan
    Neurosci Lett 509:116-20. 2012
    ..We obtained totally 8 clonally expanding Pax6-transfected cells. They started to express mRNAs of Brn3b, Cone-rod homeobox (Crx), pkc, CD73, rhodopsin and the γ-subunit of rod cGMP phosphodiesterase (PDEγ)...
  81. pmc Brn3a/Pou4f1 regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord
    Min Zou
    Center for Advanced Biotechnology and Medicine and Department of Pediatrics, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Dev Biol 364:114-27. 2012
    ..Given the expression of the POU homeodomain transcription factors Brn3a/Pou4f1 and Brn3b/Pou4f2 in DRG and spinal cord sensory neurons, we determined the subtype specification of DRG and spinal cord ..
  82. pmc Dynamic expression of ganglion cell markers in retinal progenitors during the terminal cell cycle
    Lev Prasov
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, United States
    Mol Cell Neurosci 50:160-8. 2012
    ..The first-born type of retinal neurons, ganglion cells (RGCs), develop through the actions of Math5 (Atoh7), Brn3b (Pou4f2) and Islet1 (Isl1) factors, whereas inhibitory amacrine and horizontal precursors require Ptf1a for ..
  83. pmc Whole number, distribution and co-expression of brn3 transcription factors in retinal ganglion cells of adult albino and pigmented rats
    Francisco M Nadal-Nicolás
    Unidad de Investigacion, Hospital Universitario Virgen de la Arrixaca Fundación para la Formación e Investigación Sanitarias de la Región de Murcia, IMIB, El Palmar, Murcia, Spain
    PLoS ONE 7:e49830. 2012
    The three members of the Pou4f family of transcription factors: Pou4f1, Pou4f2, Pou4f3 (Brn3a, Brn3b and Brn3c, respectively) play, during development, essential roles in the differentiation and survival of sensory neurons...
  84. pmc Human Müller glia with stem cell characteristics differentiate into retinal ganglion cell (RGC) precursors in vitro and partially restore RGC function in vivo following transplantation
    Shweta Singhal
    Divisions of Ocular Biology and Therapeutics University College London, Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
    Stem Cells Transl Med 1:188-99. 2012
    ..to neurotransmitters, and green fluorescent protein (GFP) expression by cells transduced with a transcriptional BRN3b-GFP reporter vector...
  85. ncbi Sequence comparison of the Caenorhabditis elegans dpy-13 and col-34 genes, and their deduced collagen products
    D M Bird
    Department of Nematology, University of California, Riverside 92521 0415
    Gene 120:261-6. 1992
    ..In particular, one region of Dpy-13, which is predicted to have secondary structure different from Col-34, is altered by the recessive dpy-13(e225) mutation...
  86. ncbi The POU domain transcription factor Brn-2: elevated expression in malignant melanoma and regulation of melanocyte-specific gene expression
    T Eisen
    Eukaryotic Transcription Laboratory, Marie Curie Research Institute, Oxted, Surrey, UK
    Oncogene 11:2157-64. 1995
    ..Using a proteolytic clipping assay, we show that the melanoma-specific octamer factor is Brn-2/N-Oct3, a POU domain protein previously known to be expressed in adult brain and in the developing nervous system...
  87. ncbi Interaction of Oct-1 with TFIIB. Implications for a novel response elicited through the proximal octamer site of the lipoprotein lipase promoter
    H Nakshatri
    Laboratory of Gene Regulation, Picower Institute for Medical Research, Manhasset, New York 11030, USA
    J Biol Chem 270:19613-23. 1995
    The ubiquitous human POU domain protein, Oct-1, and the related B-cell protein, Oct-2, regulate transcription from a variety of eukaryotic genes by binding to a common cis-acting octamer element, 5'-ATTTGCAT-3'...
  88. pmc Functional interaction between the POU domain protein Tst-1/Oct-6 and the high-mobility-group protein HMG-I/Y
    H Leger
    Zentrum für Molekulare Neurobiologie, Universitat Hamburg, Germany
    Mol Cell Biol 15:3738-47. 1995
    The POU domain protein Tst-1/Oct-6 is a transcriptional activator of human papovavirus JC virus in transient transfections...
  89. ncbi The cellular C1 factor of the herpes simplex virus enhancer complex is a family of polypeptides
    T M Kristie
    Laboratory of Viral Diseases, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:4387-94. 1995
    ..simplex virus are regulated by the specific assembly of a multiprotein enhancer complex containing the Oct-1 POU domain protein, the viral alpha-transinduction factor alpha TIF, (VP16, ICP25), and the C1 cellular factor...
  90. ncbi Brn-5 is a divergent POU domain factor highly expressed in layer IV of the neocortex
    B Andersen
    Eukaryotic Regulatory Biology Program, School of Medicine, University of California at San Diego, La Jolla 92093 0648
    J Biol Chem 268:23390-8. 1993
    We have identified rat cDNA clones that encode a POU domain protein, referred to as brain-5 (Brn-5)...
  91. ncbi Cloning, sequencing and restriction fragment length polymorphism analysis of a porcine cDNA for OCT2
    C K Tuggle
    Department of Animal Science, Iowa State University, Ames 50011
    Anim Genet 25:141-5. 1994
    ..The TaqI polymorphism was also detected by a non-POU-domain OCT2 probe, demonstrating that this RFLP is located in the OCT2 gene. This result suggests that OCT2 is likely to be a single-copy gene in swine as seen in other mammals...
  92. ncbi Pit-1 gene expression in the human pituitary and pituitary adenomas
    I Pellegrini
    INSERM U297, Marseille, France
    J Clin Endocrinol Metab 79:189-96. 1994
    The cellular basis for pituitary neoplasia is poorly understood. The POU domain protein Pit-1 is a pituitary-specific transcription factor involved in the generation, differentiation, and proliferation of three pituitary cell types: ..
  93. pmc Differential expression of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells
    A K Bhargava
    Department of Genetics, School of Medicine, Yale University, New Haven, CT 06536 0812
    Proc Natl Acad Sci U S A 90:10260-4. 1993
    ..Transient transfection of the gene encoding Oct-T1 decreased the activity of the interleukin 2 gene promoter in activated Jurkat cells. Further, there is evidence for an additional octamer-binding protein, Oct-T3, in Jurkat T cells...
  94. pmc Mouse Brn-3 family of POU transcription factors: a new aminoterminal domain is crucial for the oncogenic activity of Brn-3a
    T Theil
    Institut für Molekularbiologie und Tumorforschung IMT, Philipps Universitat Marburg, Germany
    Nucleic Acids Res 21:5921-9. 1993
    ..In addition, our data indicate that Brn-3b acts as an inhibitor of Brn-3a(l) activity...
  95. ncbi SpOct, a gene encoding the major octamer-binding protein in sea urchin embryos: expression profile, evolutionary relationships, and DNA binding of expressed protein
    B R Char
    Department of Biochemistry and Kenneth R Norris Cancer Hospital and Research Institute, University of Southern California School of Medicine, Los Angeles 90033
    Dev Biol 158:350-63. 1993
    ..1992) showed that expression of the alpha H2B gene in blastula-stage embryos was entirely dependent on an octamer element. Together, these data strongly suggest that SpOct may be the key regulator of the alpha H2B gene...
  96. ncbi XLPOU-60, a Xenopus POU-domain mRNA, is oocyte-specific from very early stages of oogenesis, and localised to presumptive mesoderm and ectoderm in the blastula
    T Whitfield
    Wellcome CRC Institute of Cancer and Developmental Biology, Cambridge, United Kingdom
    Dev Biol 155:361-70. 1993
    ..Localisation of the transcript in the embryo may indicate that XLPOU-60 is also required for the initiation of mesoderm- and ectoderm-specific patterns of transcription in the embryo...
  97. ncbi Short isoform of POU factor Brn-3b can form a heterodimer with Brn-3a that is inactive for octamer motif binding
    T Theil
    Institute für Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    J Biol Chem 270:30958-64. 1995
    ..Therefore, we hypothesize that Brn-3b(s) can act as a direct antagonist of Brn-3a(1) by inhibiting its DNA binding through the formation of an inactive hetero-oligomeric complex...
  98. ncbi Retina-derived POU-domain factor-1: a complex POU-domain gene implicated in the development of retinal ganglion and amacrine cells
    H Zhou
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 16:2261-74. 1996
    ..These data suggest that RPF-1 is likely to be involved in early steps in the differentiation of amacrine and ganglion cells...
  99. pmc POU domain factor Brn-3b is required for the development of a large set of retinal ganglion cells
    L Gan
    Department of Biochemistry and Molecular Biology, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Proc Natl Acad Sci U S A 93:3920-5. 1996
    ..In Brn-3b (-/-) mice other neurons within the retina and brain are minimally or not at all affected. These experiments indicate that Brn-3b plays an essential role in the development of specific ganglion cell types...
  100. ncbi A single amino acid change converts an inhibitory transcription factor into an activator
    S J Dawson
    Department of Molecular Pathology, University College London Medical School, United Kingdom
    J Biol Chem 271:11631-3. 1996
    ..Hence a single amino acid difference determines the difference between an activator and a repressor in the Brn-3 family...
  101. pmc P-OTX: a PIT-1-interacting homeodomain factor expressed during anterior pituitary gland development
    D P Szeto
    Biomedical Sciences Graduate Program, Howard Hughes Medical Institute, University of California, San Diego 92093 0648, USA
    Proc Natl Acad Sci U S A 93:7706-10. 1996
    ..identified on the basis of its ability to interact with the transactivation domain of the pituitary-specific POU domain protein, Pit-1...

Research Grants20

  1. Xiuqian Mu; Fiscal Year: 2015
    ..The knowledge obtained from this study will serve as guidance in future endeavors of developing preventive and therapeutic measures for these diseases. ..
  2. Deborah C Otteson; Fiscal Year: 2015
    ..We have cloned the EphA5 promoter and have data showing Pou4F2/Brn3b can activate the promoter in vitro...
  3. CELLULAR AND MOLECULAR MECHANISMS OF GLAUCOMA IN AN ANIMAL MODEL
    Richard Lee; Fiscal Year: 2009
    ..This grant's specific aims will characterize a popular animal model of glaucoma, lead to important discoveries in our understanding of glaucoma, and develop the career of the principal investigator as a clinician scientist. ..
  4. Transcriptional Regulation of Retinal Development
    Mengqing Xiang; Fiscal Year: 2012
    ..are proposed that will focus on the molecular and developmental events controlled by the Brn3b POU domain transcription factor, a crucial regulator of retinal ganglion cell (RGC) differentiation and survival, and its ..
  5. IDENTIFICATION OF GENES REGULATING RETINAL GANGLION CELL
    WILLIAM KLEIN; Fiscal Year: 2002
    ....
  6. GROWTH FACTORS IN MAMMALIAN EYE DEVELOPMENT
    Jeffrey Bennett; Fiscal Year: 2002
    ..These studies should provide novel insight into the molecular genetics of eye development, potentially broaden our understanding of degenerative eye disorders and open new avenues for their treatment with growth factors. ..
  7. Math5 Regulatory Pathway in Retinal Development
    Lin Gan; Fiscal Year: 2010
    ..we have shown that Math5 positively regulates the expression of RGC- specific transcription factors like Brn3b and Isl1, and negatively regulates non-RGC factors such as Bhlhb5, NeuroD, Math3, and Ngn2...
  8. EXPRESSION OF SNRNA GENES
    Arne Stenlund; Fiscal Year: 2006
    ..sequence element or DSE, which activates transcription and contains an octamer sequence that recruits the POU domain protein Oct-1 and a so-called SPH site that recruits the zinc finger protein Staf(ZNF143)...
  9. Selective Silencing of Mammalian Neurons: Proof of Mice
    Henry Lester; Fiscal Year: 2004
    ..The assay is pain. The second, for retinal ganglion cells, will employ the Brn3b promoter. The assays are visual behavior and visual evoked potentials...
  10. Brn-3 Transcription Factors in Retinal Development
    Lin Gan; Fiscal Year: 2007
    ..Transgenic approaches will be used to investigate their roles in retinal development, particularly the axon pathfinding and survival of RGCs. ..
  11. Roles of Homeodomain Factors in Neural Development
    Mengqing Xiang; Fiscal Year: 2004
    ..We have demonstrated that the POU homeodomain transcription factors Brn3a and Brn3b play essential roles during sensorineural development...
  12. Inducible Gene Expression in Retinal Ganglion Cells
    John Kerrison; Fiscal Year: 2004
    ..containing retinal ganglion cell development as evidenced by a 70% reduction in retinal ganglion cell number in Brn3b knockout mice. The final specific aim is to ascertain the network of genes regulated by Brn3b using microarrays...
  13. GENETIC CONTROL OF RETINA SPECIFICATION
    Graeme Mardon; Fiscal Year: 2005
    ..3. Place Dach1 and Dach2 in a retinal hierarchy with Pax6, Math5, and Brn3b. These studies are required to characterize the relationships between genes operating during retinal development...
  14. POU PROTEINS IN NEURAL DEVELOPMENT
    Greg Lemke; Fiscal Year: 1999
    ..We wish to understand the role of one particular POU domain protein, specifically with regard to the determination of neural cell fate and the timing of neural cell ..
  15. Genetic technologies for visualizing neuronal structure
    Jeremy Nathans; Fiscal Year: 2007
    ..ganglion cell morphologies in two lines of transcription factor gene knock-out mice (the POU-homeodomain genes Brn3b and Brn3c), and (b) identifying the spatial distribution of pre- and post-synaptic sites in single identified ..
  16. GENETIC ANALYSIS OF PAX3 INDUCTION
    STEVEN PRUITT; Fiscal Year: 2003
    ..These studies will move our understanding of how DV pattern is established, and overlaid by AP patterning mechanisms, to localize Pax3 expression in mammals to a new level of resolution. ..
  17. Neural Stem Cell Survival and Function in Aging Mice an*
    STEVEN PRUITT; Fiscal Year: 2004
    ..The ability to monitor the fates of neural stem cell progeny in vivo will allow us to determine if age- related changes in the differentiation potentials of these cells occur. ..
  18. Comprehensive Protein Interaction Mapping in Mammals
    STEVEN PRUITT; Fiscal Year: 2004
    ..Based on the utility of similar protein interaction maps in model organisms, this work can be expected to provide an enormous wealth of insight into the function of mammalian cells. ..
  19. Development of a high throughput gene trap vetor
    STEVEN PRUITT; Fiscal Year: 2003
    ..The methodologies and reagents developed here will be fully sufficient to allow the rapid establishment of a random sequence tag insertion library of 100,000 or more events in which the location of each insertion sites is known. ..
  20. TRANSCRIPTIONAL REGULATION OF BRAIN DEVELOPMENT
    Eric Turner; Fiscal Year: 2002
    ..0 gene locus in transgenic mice. (3) Compare the functional DNA recognition sites of Brn-3.0 to those of other POU proteins expressed in the brain to begin to decipher the transcriptional genetic code of the nervous system. ..