beta-actin

Summary

Gene Symbol: beta-actin
Description: actin beta
Alias: BRWS1, PS1TP5BP1, actin, cytoplasmic 1, PS1TP5-binding protein 1, beta cytoskeletal actin
Species: human

Top Publications

  1. ncbi Expression of the hepatic insulin receptor gene in the rat during postnatal development
    E Tozzo
    , Centre Hospitalier Necker Enfants Malades, Paris, France
    Horm Metab Res 27:163-8. 1995
  2. pmc beta-Actin messenger RNA localization and protein synthesis augment cell motility
    E H Kislauskis
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655, USA
    J Cell Biol 136:1263-70. 1997
  3. ncbi A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expression
    T Kimura
    Department of Microbiology, Kansai Medical University, Osaka, Japan
    Biochimie 78:1075-80. 1996
  4. ncbi Actin deficiency induces cofilin phosphorylation: proteome analysis of HeLa cells after beta-actin gene silencing
    Ning Liu
    Bio Rad Laboratories, Inc, Hercules, California 94547, USA
    Cell Motil Cytoskeleton 64:110-20. 2007
  5. pmc Beta-actin association with endothelial nitric-oxide synthase modulates nitric oxide and superoxide generation from the enzyme
    Dmitry Kondrikov
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 285:4319-27. 2010
  6. pmc A protein ballet around the viral genome orchestrated by HIV-1 reverse transcriptase leads to an architectural switch: from nucleocapsid-condensed RNA to Vpr-bridged DNA
    Sébastien Lyonnais
    AIDS Research Group, IDIBAPS, E 08036 Barcelona, Spain
    Virus Res 171:287-303. 2013
  7. pmc N-terminal Slit2 inhibits HIV-1 replication by regulating the actin cytoskeleton
    Appakkudal R Anand
    Department of Pathology, Ohio State University Wexner Medical Center, 460 W 12th Avenue, 810 Biological Research Tower, Columbus, OH 43210, USA
    Retrovirology 10:2. 2013
  8. pmc Gelsolin activity controls efficient early HIV-1 infection
    Laura García-Expósito
    Cellular and Viral Immunology Lab, Department of Physical Medicine and Pharmacology, School of Medicine, University of La Laguna, Campus de Ofra s n, Tenerife 38071, Spain
    Retrovirology 10:39. 2013
  9. pmc A posttranslational modification of beta-actin contributes to the slow dissociation of the spectrin-protein 4.1-actin complex of irreversibly sickled cells
    A Shartava
    Department of Structural and Cellular Biology, University of South Alabama College of Medicine, Mobile 36688
    J Cell Biol 128:805-18. 1995
  10. ncbi Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle
    S Yokota
    HSP Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto, Japan
    Eur J Biochem 268:4664-73. 2001

Research Grants

  1. Alzheimer Vaccines: Noninvasive Vaccination by DNA-Base*
    Ken Ichiro Fukuchi; Fiscal Year: 2005
  2. Robert H Singer; Fiscal Year: 2015
  3. Harish Vasudevan; Fiscal Year: 2015
  4. Changwei Liu; Fiscal Year: 2014
  5. Regulating retinal microvascular morphogenesis
    Ira M Herman; Fiscal Year: 2010
  6. BETA-ACTIN MRNA LOCALIZATION
    GARY BASSELL; Fiscal Year: 2002
  7. MOLECULAR BASIS FOR ETHANOL-INDUCED HYPOTHYROIDISM
    Robert Zoeller; Fiscal Year: 1993
  8. Transgenic Mouse Model for Echovirus Pathogenesis
    VINCENT RACANIELLO; Fiscal Year: 2004
  9. ROLES OF MICROFILAMENT PROTEINS IN NEOPLASIA/METASTASIS
    John Leavitt; Fiscal Year: 1993
  10. METALS EFFECT ON HEME METABOLISM
    MAHIN MAINES; Fiscal Year: 1999

Detail Information

Publications283 found, 100 shown here

  1. ncbi Expression of the hepatic insulin receptor gene in the rat during postnatal development
    E Tozzo
    , Centre Hospitalier Necker Enfants Malades, Paris, France
    Horm Metab Res 27:163-8. 1995
    ..5 and 7.5 kb. When normalized to beta actin mRNA, insulin receptor mRNA levels increased 4-fold between 1 and 15 days, remained stable between 15 and 30 ..
  2. pmc beta-Actin messenger RNA localization and protein synthesis augment cell motility
    E H Kislauskis
    Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655, USA
    J Cell Biol 136:1263-70. 1997
    ..The serum induction of cell movement was also inhibited by antizipcode ODNs. These observations support the hypothesis that beta-actin mRNA localization and consequent protein synthesis augment cell motility...
  3. ncbi A role for Rev in the association of HIV-1 gag mRNA with cytoskeletal beta-actin and viral protein expression
    T Kimura
    Department of Microbiology, Kansai Medical University, Osaka, Japan
    Biochimie 78:1075-80. 1996
    ....
  4. ncbi Actin deficiency induces cofilin phosphorylation: proteome analysis of HeLa cells after beta-actin gene silencing
    Ning Liu
    Bio Rad Laboratories, Inc, Hercules, California 94547, USA
    Cell Motil Cytoskeleton 64:110-20. 2007
    ..This study also demonstrates that the combination of RNA interference and 2-dimensional gel electrophoresis technologies provides a valuable method to study protein interactions in a specific cellular pathway...
  5. pmc Beta-actin association with endothelial nitric-oxide synthase modulates nitric oxide and superoxide generation from the enzyme
    Dmitry Kondrikov
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 285:4319-27. 2010
    ..Modulation of NO and superoxide formation from eNOS by beta-actin plays an important role in endothelial function...
  6. pmc A protein ballet around the viral genome orchestrated by HIV-1 reverse transcriptase leads to an architectural switch: from nucleocapsid-condensed RNA to Vpr-bridged DNA
    Sébastien Lyonnais
    AIDS Research Group, IDIBAPS, E 08036 Barcelona, Spain
    Virus Res 171:287-303. 2013
    ....
  7. pmc N-terminal Slit2 inhibits HIV-1 replication by regulating the actin cytoskeleton
    Appakkudal R Anand
    Department of Pathology, Ohio State University Wexner Medical Center, 460 W 12th Avenue, 810 Biological Research Tower, Columbus, OH 43210, USA
    Retrovirology 10:2. 2013
    ..Slit2 is a ~ 200 kDa secreted glycoprotein that has been recently shown to regulate immune functions. However, not much is known about its role in HIV (human immunodeficiency virus)-1 pathogenesis...
  8. pmc Gelsolin activity controls efficient early HIV-1 infection
    Laura García-Expósito
    Cellular and Viral Immunology Lab, Department of Physical Medicine and Pharmacology, School of Medicine, University of La Laguna, Campus de Ofra s n, Tenerife 38071, Spain
    Retrovirology 10:39. 2013
    ..However, it remains unclear whether the dynamic processing of F-actin and the amount of cortical actin available during the initial virus-cell contact are required to such events...
  9. pmc A posttranslational modification of beta-actin contributes to the slow dissociation of the spectrin-protein 4.1-actin complex of irreversibly sickled cells
    A Shartava
    Department of Structural and Cellular Biology, University of South Alabama College of Medicine, Mobile 36688
    J Cell Biol 128:805-18. 1995
    ....
  10. ncbi Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle
    S Yokota
    HSP Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto, Japan
    Eur J Biochem 268:4664-73. 2001
    ..These results suggest that the content of alpha and delta subunits of CCT reduces from the complete active complex in S phase cells to incomplete inactive complex in M phase cells...
  11. ncbi Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins
    Vincent C Chen
    Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada
    J Proteome Res 5:2123-34. 2006
    ....
  12. doi Essential and supporting host cell factors for HIV-1 budding
    Aurelien Dordor
    Unit of Virus Host Cell Interactions UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France
    Future Microbiol 6:1159-70. 2011
    ..Here we review recent advances in understanding the role of host cell factors recruited by Gag during HIV-1 assembly and budding...
  13. pmc HIV-1 Nef impairs heterotrimeric G-protein signaling by targeting Gα(i2) for degradation through ubiquitination
    Prabha Chandrasekaran
    Laboratory of Molecular Immunology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:41481-98. 2012
    ..By specifically degrading Gα(i2), Nef directly subverts leukocyte migration and homing. Impaired trafficking and homing of HIV Nef-expressing lymphocytes probably contributes to early immune dysfunction following HIV infection...
  14. pmc The nucleocapsid domain of Gag is dispensable for actin incorporation into HIV-1 and for association of viral budding sites with cortical F-actin
    Sarah Stauffer
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    J Virol 88:7893-903. 2014
    ..Furthermore, actin was not enriched in the virus compared to the average levels in the respective producing cell. Our data argue against a specific recruitment of actin to HIV-1 budding sites by the nucleocapsid domain of Gag...
  15. ncbi HIV-1 protease cleaves actin during acute infection of human T-lymphocytes
    L D Adams
    Biochemistry Unit, Upjohn Laboratories, Kalamazoo, MI 49001
    AIDS Res Hum Retroviruses 8:291-5. 1992
    ..Hydrolysis by the HIV-1 protease of physiologically important host cellular proteins during infection may have important consequences relative to viral pathogenesis...
  16. pmc Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF
    H Zhang
    The Lineberger Comprehensive Cancer Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Curr Biol 9:1271-4. 1999
    ..These findings suggest that an important function of gp41C is to modulate the activity of p115-RhoGEF and they thus reveal a new potential anti-HIV-1 target...
  17. ncbi HIV-1 Nef triggers Vav-mediated signaling pathway leading to functional and morphological differentiation of dendritic cells
    Maria Giovanna Quaranta
    Department of Immunology, Istituto Superiore di Sanità Viale Regina Elena, 299 00161 Rome, Italy
    FASEB J 17:2025-36. 2003
    ..Our results highlight how Nef may enhance T lymphocyte activation, thus fostering virus dissemination, manipulating the DC arm of the immune response...
  18. pmc Guanidine alkaloid analogs as inhibitors of HIV-1 Nef interactions with p53, actin, and p56lck
    Allison Olszewski
    Department of Chemistry, University of California, Irvine, CA 92697 2025, USA
    Proc Natl Acad Sci U S A 101:14079-84. 2004
    ..Although compounds that are effective in vitro proved to be too cytotoxic for cellular assays, the reported Nef inhibitors provide proof-of-concept for disrupting a new HIV target and offer useful leads for drug development...
  19. pmc Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice
    Zhenglin Yang
    Department of Ophthalmology and Visual Science, Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah, USA
    J Clin Invest 118:2908-16. 2008
    ..Collectively, our results identify what we believe to be a novel complex involved in photoreceptor disk morphogenesis and indicate a possible role for PROM1 and PCDH21 in macular degeneration...
  20. pmc Nuclear myosin II regulates the assembly of preinitiation complex for ICAM-1 gene transcription
    Qingjie Li
    Department of Internal Medicine, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1083, USA
    Gastroenterology 137:1051-60, 1060.e1-3. 2009
    ..We do not know whether myosin II and actins in the nucleus function as motors to generate relative motion, such as that between RNA polymerase II holoenzyme and DNA, for assembly of the preinitiation complex...
  21. pmc Cloning of human erythroid dematin reveals another member of the villin family
    A P Rana
    Department of Biomedical Research, St Elizabeth s Hospital, Tufts University School of Medicine, Boston, MA 02135
    Proc Natl Acad Sci U S A 90:6651-5. 1993
    ..The widespread expression of dematin transcripts in human tissues suggests that dematin and its homologues may substitute for villin in villin-negative tissues to regulate actin reorganization by a phosphorylation-regulated mechanism...
  22. ncbi HIV-1 Gag protein associates with F-actin present in microfilaments
    O Rey
    Department of Pediatrics, School of Medicine, University of California at Los Angeles 90095, USA
    Virology 220:530-4. 1996
    ..In vivo and in vitro analyses of this interaction indicated that the unprocessed Gag polyprotein is capable of association with polymerized actin (F-actin). Binding of Gag to F-actin may be involved in the assembly or budding of HIV-1...
  23. pmc Cytoskeletal proteins inside human immunodeficiency virus type 1 virions
    D E Ott
    AIDS Vaccine Program, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702 1201, USA
    J Virol 70:7734-43. 1996
    ..The presence of these fragments, as well as the incorporation of a few specific cytoskeletal proteins into virions, suggests an active interaction between cytoskeletal and viral proteins...
  24. ncbi Beta cap73: a novel beta actin-specific binding protein
    C B Shuster
    Program in Cell, Molecular, and Developmental Biology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Cell Motil Cytoskeleton 35:175-87. 1996
    ..Cell Biol. 128:837-848]. To identify the beta actin-specific binding protein that fosters ezrin-beta actin interactions, we developed an isoactin affinity ..
  25. pmc Establishment of a functional human immunodeficiency virus type 1 (HIV-1) reverse transcription complex involves the cytoskeleton
    A Bukrinskaya
    Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA
    J Exp Med 188:2113-25. 1998
    ....
  26. ncbi Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling
    K Zhao
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, California 94305 5323, USA
    Cell 95:625-36. 1998
    ..This work indicates that membrane signals control the activity of the mammalian SWI/SNF or BAF complex and demonstrates a direct interface between signaling and chromatin regulation...
  27. pmc Actin associates with the nucleocapsid domain of the human immunodeficiency virus Gag polyprotein
    T Wilk
    Structural Biology Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    J Virol 73:1931-40. 1999
    ..The same result was obtained when mature HIV-1 particles were disrupted with detergent. Taken together, these results indicate that actin molecules are associated with the NC domain of the viral polyprotein...
  28. pmc Interaction of the human immunodeficiency virus type 1 nucleocapsid with actin
    B Liu
    Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA
    J Virol 73:2901-8. 1999
    ....
  29. pmc A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection
    H Nunoi
    Institute of Medical Science, University of Tokyo, Minatoku, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 96:8693-8. 1999
    ..Heterozygous expression of mutant beta-actin in neutrophils and other cells of this patient may act in a dominant-negative fashion to adversely affect cellular activities dependent on the function of nonmuscle actin...
  30. pmc The golgi-associated COPI-coated buds and vesicles contain beta/gamma -actin
    F Valderrama
    Departament de Biologia Cellular, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, 08036 Barcelona, Spain
    Proc Natl Acad Sci U S A 97:1560-5. 2000
    ....
  31. pmc Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts
    M Sakaguchi
    Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700 8558, Japan
    J Cell Biol 149:1193-206. 2000
    ..These data indicate the possible involvement of nuclear S100C in the contact inhibition of cell growth...
  32. ncbi Specific changes to the mechanism of cell locomotion induced by overexpression of beta-actin
    M Peckham
    School of Biomedical Sciences, Worsley Building, University of Leeds, Leeds LS2 9JT, UK
    J Cell Sci 114:1367-77. 2001
    ....
  33. ncbi HIV type 1 Gag and nucleocapsid proteins: cytoskeletal localization and effects on cell motility
    F J Ibarrondo
    Department of Pediatrics, UCLA School of Medicine, Los Angeles, California 90095, USA
    AIDS Res Hum Retroviruses 17:1489-500. 2001
    ..These data suggest that interactions between HIV-1 Gag and actin in infected cells enhance cell motility. Ultimately this enhanced motility of infected cells could promote the dissemination of virus into the brain and other tissues...
  34. ncbi Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin
    Ryusuke Niwa
    Department of Molecular Genetics, The Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Cell 108:233-46. 2002
    ..Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo...
  35. ncbi Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  36. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  37. pmc Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory proteins
    Patrick B Allen
    Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06508, USA
    Proc Natl Acad Sci U S A 101:7187-92. 2004
    ..The current findings present a mechanism by which PP1 may be directed toward neuronal substrates associated with the actin cytoskeleton...
  38. pmc Disruption of the actin cytoskeleton can complement the ability of Nef to enhance human immunodeficiency virus type 1 infectivity
    Edward M Campbell
    Department of Microbiology and Immunology, University of Illinois at Chicago, 835 S Wolcott, Chicago, IL 60612, USA
    J Virol 78:5745-55. 2004
    ..The results presented here suggest that Nef functions to allow the HIV genome to penetrate the cortical actin network, a known barrier for intracellular parasitic organisms...
  39. ncbi Nuclear actin and myosin I are required for RNA polymerase I transcription
    Vlada V Philimonenko
    Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic
    Nat Cell Biol 6:1165-72. 2004
    ..The association with Pol I requires phosphorylation of TIF-IA at Ser 649 by RSK kinase, indicating a role for NMI in the growth-dependent regulation of rRNA synthesis...
  40. ncbi Beta-actin in human colon adenocarcinoma cell lines with different metastatic potential
    Dorota Nowak
    Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland
    Acta Biochim Pol 52:461-8. 2005
    ..beta-actin subcellular localization was done by immunofluorescence staining with monoclonal anti-beta actin antibodies...
  41. pmc Requirement for an intact T-cell actin and tubulin cytoskeleton for efficient assembly and spread of human immunodeficiency virus type 1
    Clare Jolly
    The Sir William Dunn School of Pathology, The University of Oxford, Oxford OX1 3RE, United Kingdom
    J Virol 81:5547-60. 2007
    ..Based on these data, we propose that HIV-1 requires both actin and tubulin components of the T-cell cytoskeleton to direct its assembly and budding and to elaborate a functional VS...
  42. pmc The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the beta-actin mRNA in a U-rich-element-dependent manner
    Virginie Dormoy-Raclet
    Department of Biochemistry, and McGill Cancer Center, McGill University, McIntyre Building, Room 904, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada H3G 1Y6
    Mol Cell Biol 27:5365-80. 2007
    ....
  43. pmc Selective targeting of ITK blocks multiple steps of HIV replication
    Julie A Readinger
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:6684-9. 2008
    ..Our data suggest that inhibition of ITK blocks HIV infection by affecting multiple steps of HIV replication...
  44. pmc HIV envelope-CXCR4 signaling activates cofilin to overcome cortical actin restriction in resting CD4 T cells
    Alyson Yoder
    Department of Molecular and Microbiology, George Mason University, Manassas, VA 20110, USA
    Cell 134:782-92. 2008
    ..Conversely, induction of active cofilin greatly facilitates it. These findings shed light on viral exploitation of cellular machinery in resting T cells, where chemokine receptor signaling becomes obligatory...
  45. doi Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity
    Vera Dugina
    Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow, Russia
    J Cell Sci 122:2980-8. 2009
    ..Our results reveal new aspects of beta- and gamma-actin organization that support their functional diversity...
  46. pmc Human immunodeficiency virus type 1 envelope gp120-induced partial T-cell receptor signaling creates an F-actin-depleted zone in the virological synapse
    Gaia Vasiliver-Shamis
    Program in Molecular Pathogenesis, Marty and Helen Kimmel Center for Biology and Medicine, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Virol 83:11341-55. 2009
    ..We propose a model in which the F-actin-depleted zone formed within the target CD4 T cell enhances the reception of virions by releasing the physical barrier for HIV-1 entry and facilitating postentry events...
  47. pmc Retroviral assembly and budding occur through an actin-driven mechanism
    Micha Gladnikoff
    Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
    Biophys J 97:2419-28. 2009
    ..The results of this study show that retroviruses utilize the cell cytoskeleton to expedite their assembly and budding...
  48. doi Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4
    Masahiro Kumeta
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8501, Japan
    J Cell Sci 123:1020-30. 2010
    ....
  49. pmc The PDZ-adaptor protein syntenin-1 regulates HIV-1 entry
    Mónica Gordón-Alonso
    Servicio de Inmunologia, Instituto de Investigación Sanitaria de la Princesa, Hospital Universitario de la Princesa, 28006 Madrid, Spain
    Mol Biol Cell 23:2253-63. 2012
    ..Once the virus has entered the target cell, syntenin-1 polarization toward the viral nucleocapsid is lost, suggesting a spatiotemporal regulatory role of syntenin-1 in actin remodeling, PIP(2) production, and the dynamics of HIV-1 entry...
  50. pmc The trinity of the cortical actin in the initiation of HIV-1 infection
    Mark Spear
    National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University, Manassas, VA 20110, USA
    Retrovirology 9:45. 2012
    ..It is the purpose of this review to examine, in detail, the manifestation of viral dependence on the actin cytoskeleton, and present a model of how HIV utilizes actin dynamics to initiate infection...
  51. doi ACTB in cancer
    Chunmei Guo
    Department of Biotechnology, Dalian Medical University, Dalian 116044, China
    Clin Chim Acta 417:39-44. 2013
    ..The current review explores relevant mechanisms, integrates current understandings, and provides suggestions for future studies of the roles of ACTB in tumors...
  52. ncbi The cleavage of host cell proteins by HIV-1 protease
    J Snasel
    Department of Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Folia Biol (Praha) 42:227-30. 1996
  53. pmc Human immunodeficiency virus type 1 Tat regulates endothelial cell actin cytoskeletal dynamics through PAK1 activation and oxidant production
    Ru Feng Wu
    The University of Texas Southwestern Medical Center and the Dallas Veterans Administration Medical Center, Dallas, Texas 75216, USA
    J Virol 78:779-89. 2004
    ..We conclude that Tat induces actin cytoskeletal rearrangements through PAK1 and downstream activation of the endothelial NADPH oxidase...
  54. pmc A role for beta-actin in RNA polymerase III transcription
    Ping Hu
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA
    Genes Dev 18:3010-5. 2004
    ..Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription...
  55. ncbi HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET
    Emma Poole
    Department of Medicine, University of Cambridge, Addenbrooke s Hospital, Cambridge, CB2 2QQ, UK
    Traffic 6:741-55. 2005
    ..HIV-1 Psi thus acts as a subcellular localisation signal as well as a high-affinity-binding site for Gag...
  56. ncbi Modifications in the human T cell proteome induced by intracellular HIV-1 Tat protein expression
    Mayte Coiras
    AIDS Immunopathology Unit, National Centre of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    Proteomics 6:S63-73. 2006
    ..In conclusion, in addition to its role in viral mRNA elongation, the proteomic approach has provided insight into the way that Tat modifies host cell gene expression...
  57. ncbi HIV-1 replication from after cell entry to the nuclear periphery
    David Warrilow
    Division of Immunology and Infectious Disease, Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    Curr HIV Res 5:293-9. 2007
    ..An actin-transport process may then facilitate the remaining short journey to the nuclear membrane...
  58. ncbi Actin, troponin C, Alzheimer amyloid precursor protein and pro-interleukin 1 beta as substrates of the protease from human immunodeficiency virus
    A G Tomasselli
    Upjohn Laboratories, Upjohn Company, Kalamazoo, Michigan 49001
    J Biol Chem 266:14548-53. 1991
    ....
  59. ncbi Non-viral cellular substrates for human immunodeficiency virus type 1 protease
    R L Shoeman
    Max Planck Institut für Zellbiologie, Ladenburg Heidelberg, Germany
    FEBS Lett 278:199-203. 1991
    ....
  60. pmc The large subunit of HIV-1 reverse transcriptase interacts with beta-actin
    M Hottiger
    Department of Veterinary Biochemistry, University of Zurich Irchel, Switzerland
    Nucleic Acids Res 23:736-41. 1995
    ..The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions...
  61. pmc Indirect association of ezrin with F-actin: isoform specificity and calcium sensitivity
    C B Shuster
    Program in Cell, Molecular, and Developmental Biology, Tufts University Health Science Schools, Boston, Massachusetts 02111
    J Cell Biol 128:837-48. 1995
    ..and E64, two specific inhibitors of the calcium-activated protease, calpain I, protect ezrin binding to beta actin in the presence of calcium...
  62. ncbi Association of human immunodeficiency virus Nef protein with actin is myristoylation dependent and influences its subcellular localization
    O T Fackler
    Universitatskliniken des Saarlandes, Institut für Med Mikrobiologie und Hygiene, Abt Virologie, Homburg Saar, Germany
    Eur J Biochem 247:843-51. 1997
    ..This novel interaction of HIV-1 Nef with actin provides insight into the association of Nef with cellular structures and reveals general differences in the interactions of the Nef proteins from HIV-1 and HIV-2...
  63. ncbi In vitro interaction of the carboxy-terminal domain of lamin A with actin
    A M Sasseville
    Centre de recherche du Centre Hospitalier de l Université de Montréal and Institut du cancer de Montréal, Que, Canada
    FEBS Lett 425:485-9. 1998
    ..This result suggests that an actin-based molecular motor linked to the lamina could be involved in the movement of chromatin domains...
  64. pmc Lasp-1, a novel type of actin-binding protein accumulating in cell membrane extensions
    V Schreiber
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP Strasbourg, Illkirch, France
    Mol Med 4:675-87. 1998
    ..Altogether, our results show that Lasp-1 defines a new type of actin-binding protein and suggest that the protein may play a role in a signaling pathway involved in the organization of the cytoskeleton...
  65. ncbi Rev-dependent association of the intron-containing HIV-1 gag mRNA with the nuclear actin bundles and the inhibition of its nucleocytoplasmic transport by latrunculin-B
    T Kimura
    Departments of Microbiology and Physiology, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
    Genes Cells 5:289-307. 2000
    ..A hallmark of HIV-1 gene expression is that unspliced genomic RNA, which also acts as mRNA for the expression of Gag/Pol, is exported to the cytoplasm. Rev directs this transport through the nuclear export signal (NES)...
  66. ncbi Association of emerin with nuclear and cytoplasmic actin is regulated in differentiating myoblasts
    Giovanna Lattanzi
    ITOI CNR, Unit of Bologna, c o IOR, Via di Barbiano, 1 10, I 40136 Bologna, Italy
    Biochem Biophys Res Commun 303:764-70. 2003
    ..These data provide evidence of a modulation of emerin-actin interaction in muscle cells, possibly through differentiation-related stimuli...
  67. ncbi Silencing of the Tropomyosin-1 gene by DNA methylation alters tumor suppressor function of TGF-beta
    Andrea E Varga
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Oncogene 24:5043-52. 2005
    ..Together these results suggest that epigenetic suppression of TPM1 may alter TGF-beta tumor suppressor function and contribute to metastatic properties of tumor cells...
  68. ncbi Regulating the functions of the HIV-1 matrix protein
    Anna C Hearps
    Nuclear Signalling Laboratory, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
    AIDS Res Hum Retroviruses 23:341-6. 2007
    ..J Virol 2005;79:13028-13036) confirms the importance of this protein for HIV infection and highlights a potentially new avenue in multivalent drug therapy...
  69. ncbi Proteomic characterization of protein phosphatase 1 complexes in ischemia-reperfusion and ischemic tolerance
    Cristina Cid
    Department of Investigation, Hospital Ramon y Cajal, Madrid, Spain
    Proteomics 7:3207-18. 2007
    ..It supports a potential role of PP1 in IR stress and as a target of the endogenous protective mechanisms induced by IT...
  70. pmc Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL
    Stephane Mouilleron
    Structural Biology Laboratory, Cancer Research UK, London Research Institute, London, UK
    EMBO J 27:3198-208. 2008
    ..Comparison with other actin-binding proteins reveals an unexpected similarity to the vitamin-D-binding protein, extending the G-actin-binding protein repertoire...
  71. pmc Lentiviral Nef proteins utilize PAK2-mediated deregulation of cofilin as a general strategy to interfere with actin remodeling
    Bettina Stolp
    Department of Infectious Diseases, Virology, University of Heidelberg, INF 324, 69120 Heidelberg, Germany
    J Virol 84:3935-48. 2010
    ....
  72. doi HIV-1 gp41 ectodomain enhances Cryptococcus neoformans binding to human brain microvascular endothelial cells via gp41 core-induced membrane activities
    Sheng He Huang
    Division of Infectious Diseases, Saban Research Institute, Childrens Hospital Los Angeles, University of Southern California, Keck School of Medicine, Los Angeles, CA 90027, USA
    Biochem J 438:457-66. 2011
    ..neoformans binding to HBMECs via gp41 core domain-induced membrane activities, revealing a potential mechanism of invasion for this pathogenic fungus into the brain tissues of HIV-1-infected patients...
  73. pmc LIM kinase 1 - dependent cofilin 1 pathway and actin dynamics mediate nuclear retinoid receptor function in T lymphocytes
    Mohammad Ishaq
    Laboratory of Molecular Cell Biology, SAIC Frederick, National Cancer Institute, Frederick, MD 21702, USA
    BMC Mol Biol 12:41. 2011
    ..Here we have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation...
  74. pmc A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia
    Vincent Procaccio
    Center for Molecular and Mitochondrial Medicine and Genetics, University of California, Irvine, CA 92697, USA
    Am J Hum Genet 78:947-60. 2006
    ..These findings suggest that mutations in nonmuscle actins may be associated with a broad spectrum of developmental malformations and/or neurological abnormalities such as dystonia...
  75. ncbi Effects of protein kinase C alpha overexpression on A7r5 smooth muscle cell proliferation and differentiation
    S Wang
    Department of Physiology, Marshall University School of Medicine, Huntington, West Virginia 25755, USA
    Exp Cell Res 236:117-26. 1997
    ..These findings suggest that PKC alpha regulates growth and differentiation of A7r5 smooth muscle cells and that these changes might result from altered expression/function of AP-1 transcription factors...
  76. ncbi Expression of transforming growth factor-beta1 and transforming growth factor-beta Type-II receptor mRNA in papillary thyroid carcinoma
    H Matoba
    First Department of Internal Medicine, Toho University School of Medicine, Tokyo, Japan
    Horm Metab Res 30:624-8. 1998
    ..The reduction of TGF-beta Type-II receptor mRNA may play a role in the pathogenesis of papillary thyroid carcinoma...
  77. pmc A beta-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies
    C Bonnerot
    , , Paris
    Proc Natl Acad Sci U S A 84:6795-9. 1987
    ..Thus, transcriptional specificity must already be present at the stage of activation of the embryonic genome...
  78. ncbi Expression of an orphan nuclear receptor DAX-1 in human pituitary adenomas
    S Ikuyama
    Third Department of Internal Medicine, Kyushu University Faculty of Medicine, Fukuoka, Japan
    Clin Endocrinol (Oxf) 48:647-54. 1998
    ..CONCLUSIONS: The results shown here indicate that DAX-1 is expressed in the majority of human pituitary adenomas of gonadotrophic origin in parallel with Adrenal-4-binding protein/steroidogenic factor-1...
  79. ncbi Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes
    K Gorzelniak
    Franz-Volhard-Klinik, , , Germany
    Horm Metab Res 33:625-7. 2001
    ..Only GAPDH and transferrin receptor gene expression levels did not change under any of the stimuli tested, thus appeared best suited for gene expression studies in human adipose cells across a wide range of experimental settings...
  80. pmc Oncogene expression in vivo by ovarian adenocarcinomas and mixed-mullerian tumors
    B M Kacinski
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510
    Yale J Biol Med 62:379-92. 1989
    ..The authors discuss the role that tumor epithelial cell expression of the fms gene product might play in the auto- and paracrine control of growth and dissemination of ovarian adenocarcinomas...
  81. ncbi Expression of three negative regulators of CYP17 gene transcription in adrenocortical cells
    T Lehmann
    Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznan, Poland
    Endocr Res 26:1019-26. 2000
    ..Our results suggest that in the adrenocortical cells in culture, the expression of DAX1 and COUP-TF is constitutive, whereas N-CoR expression might be inducible...
  82. ncbi Angiotensin converting enzyme inhibition corrects Na+/H+ exchanger overactivity in essential hypertension
    A Fortuno
    Vascular Pathophysiology Unit, School of Medicine, University of Navarra, Pamplona, Spain
    Am J Hypertens 10:84-93. 1997
    ..This effect appears to be independent of changes in the expression of the mRNA encoding for the NHE-1 isoform of the exchanger...
  83. ncbi The effect of interferon-alpha2b on an in vitro model Dupuytren's contracture
    J L Sanders
    Department of Surgery, University of Alberta, Edmonton, Canada
    J Hand Surg Am 24:578-85. 1999
    ..In Dupuytren's fibroblasts, treatment with IFN-alpha2b significantly down-regulated mRNA expression for cytoplasmic beta-actin and gamma-actin...
  84. ncbi Cytochalasin D-induced actin gene expression in murine erythroleukemia cells
    C J Sympson
    Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292
    Exp Cell Res 205:225-31. 1993
    ..By contrast, a minimal 99-bp actin promoter-CAT construct containing a functional SRE did not respond to CD...
  85. doi The heterogeneous nuclear ribonucleoprotein-R is necessary for axonal beta-actin mRNA translocation in spinal motor neurons
    Michael Glinka
    Institute for Clinical Neurobiology, Zinklesweg 10, 97078 Wurzburg, Germany
    Hum Mol Genet 19:1951-66. 2010
    ..Our data suggest that hnRNP-R-mediated axonal beta-actin mRNA translocation plays an essential physiological role for axon growth and presynaptic differentiation...
  86. ncbi Overexpression of human PHEX under the human beta-actin promoter does not fully rescue the Hyp mouse phenotype
    Reinhold G Erben
    Institute of Physiology, Physiological Chemistry and Animal Nutrition, Ludwig Maximilians University, Munich, Germany
    J Bone Miner Res 20:1149-60. 2005
    ....
  87. doi FRET analysis of actin-myosin interaction in contracting rat aortic smooth muscle
    J Black
    The Joan C Edwards School of Medicine, Department of Pharmacology, Physiology and Toxicology, Marshall University, Huntington, WV 25704, USA
    Can J Physiol Pharmacol 87:327-36. 2009
    ..The results provide direct evidence of dynamic remodeling of the contractile protein during vascular smooth muscle contraction and suggest that FRET analysis may be a powerful tool for assessment of tissue protein-protein associations...
  88. pmc beta-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels
    E M Glare
    Department of Respiratory Medicine, Alfred Hospital and Monash University Medical School, Melbourne 3181, Australia
    Thorax 57:765-70. 2002
    ..We hypothesised that airway samples which differ in their cellular profiles and activation status have different levels of expression of GAPDH and beta-actin...
  89. ncbi Increased beta-actin and tubulin polymerization in regrowing axons: relationship to the conditioning lesion effect
    Linda M Lund
    VA Medical Cetner 151W, Cleveland, Ohio 44106, USA
    Exp Neurol 178:306-12. 2002
    ....
  90. ncbi Crystallization and structure determination of bovine profilin at 2.0 A resolution
    E S Cedergren-Zeppezauer
    Department of Zoological Cell Biology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden
    J Mol Biol 240:459-75. 1994
    ..In forming this cluster, Trp31 adopts a sterically strained rotamer conformation.(ABSTRACT TRUNCATED AT 400 WORDS)..
  91. pmc Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization
    Yuri Oleynikov
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Curr Biol 13:199-207. 2003
    ..The ZBP1 protein contains four KH RNA binding domains and a classic RBD RNA binding domain. It also contains a putative nuclear import and export sequence, suggesting a nuclear phase in this protein's function...
  92. ncbi Differential distribution of beta- and gamma-actin in guinea-pig cochlear sensory and supporting cells
    D N Furness
    MacKay Institute of Communication and Neuroscience, School of Life Sciences, Keele University, Staffordshire ST5 5BG, United Kingdom
    Hear Res 207:22-34. 2005
    ..Increased expression of beta-actin in outer pillar cells towards the cochlear base may contribute to the greater stiffness of this region...
  93. ncbi cDNA cloning and sequence of a new type of actin in mouse B16 melanoma
    H Sadano
    Department of Experimental Cell Research, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    J Biol Chem 263:15868-71. 1988
    ..These differences between Ax and beta-actin cDNA indicate that the Ax actin is encoded by an unique gene set, independent of beta-actin...
  94. ncbi Tumor necrosis factor-alpha causes barrier dysfunction mediated by tyrosine198 and tyrosine218 in beta-actin
    Nancy Gertzberg
    Department of Pharmaceutical Science, Albany College of Pharmacy, Albany, NY 12208, USA
    Am J Physiol Lung Cell Mol Physiol 293:L1219-29. 2007
    ..The data indicate that TNF induces an increase in endothelial permeability that is dependent on tyrosine(198) and tyrosine(218) in beta-actin...
  95. pmc Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes
    Sundaravadivel Balasubramanian
    Cardiology Division, Department of Medicine, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston, South Carolina, United States of America
    PLoS ONE 5:e11470. 2010
    ..These studies demonstrate the presence and dynamics of beta-actin in the adult cardiomyocyte and reinforce its usefulness in measuring cardiac cytoskeletal rearrangement during hypertrophic stimulation...
  96. pmc High level expression of transfected beta- and gamma-actin genes differentially impacts on myoblast cytoarchitecture
    G Schevzov
    Cell Biology Unit, Children s Medical Research Foundation, Camperdown N S W, Australia
    J Cell Biol 117:775-85. 1992
    ..In particular, we conclude that the beta- and gamma-actin genes encode functionally distinct cytoarchitectural information...
  97. doi Both beta-actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer
    Ryutaro Mori
    Department of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Prostate 68:1555-60. 2008
    ..We investigated which housekeeping gene is useful to study gene expression of paraffin embedded human tissue samples of prostate cancer...
  98. pmc Ion-dependent polymerization differences between mammalian beta- and gamma-nonmuscle actin isoforms
    Sarah E Bergeron
    Department of Biochemistry, Roy A and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 285:16087-95. 2010
    ..This difference may be particularly important in sites such as the gamma-actin-rich cochlear hair cell stereocilium where local mm calcium concentrations may exist...
  99. ncbi Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses
    Taesun Eom
    Department of Neuroscience, Rose F Kennedy Center for Mental Retardation, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:10433-44. 2003
    ..These results reveal a novel function for an mRNA localization element and its binding protein in the regulation of dendritic morphology and synaptic growth via dendritic filopodia...
  100. ncbi Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1
    Stefan Huttelmaier
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nature 438:512-5. 2005
    ..These sequential events provide both temporal and spatial control over beta-actin mRNA translation, which is important for cell migration and neurite outgrowth...
  101. ncbi Expression patterns of ubiquitin, heat shock protein 70, alpha-actin and beta-actin over the molt cycle in the abdominal muscle of marine shrimp Litopenaeus vannamei
    Jose Renato O Cesar
    Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, Hawaii
    Mol Reprod Dev 74:554-9. 2007
    ..Muscle structural alpha-actin and cytoskeletal beta-actin were increased during intermolt and premolt stages, suggesting high muscle growth during these stages in the abdominal muscle of the L. vannamei...

Research Grants97

  1. Alzheimer Vaccines: Noninvasive Vaccination by DNA-Base*
    Ken Ichiro Fukuchi; Fiscal Year: 2005
    ..The nasal membrane application procedure eliminates pain and other problems associated with needle injection, protein purification, and adjuvant modification, and so may reduce medical costs. ..
  2. Robert H Singer; Fiscal Year: 2015
    ....
  3. Harish Vasudevan; Fiscal Year: 2015
    ..By studying the interactions between PDGF signaling and SRF activity, we will build the foundation required to design innovative diagnostic and therapeutic strategies targeting these fundamental pathways. ..
  4. Changwei Liu; Fiscal Year: 2014
    ..Our study might discover a deubiquitinating enzyme as a novel regulator of the SMN complex and thus, identify a novel therapeutic target for SMA. ..
  5. Regulating retinal microvascular morphogenesis
    Ira M Herman; Fiscal Year: 2010
    ....
  6. BETA-ACTIN MRNA LOCALIZATION
    GARY BASSELL; Fiscal Year: 2002
    ..Using high resolution in situ hybridization and digital imaging microscopy methods, we have shown that beta actin mRNAs were localized to growth cones of developing dendritic and axonal processes...
  7. MOLECULAR BASIS FOR ETHANOL-INDUCED HYPOTHYROIDISM
    Robert Zoeller; Fiscal Year: 1993
    ....
  8. Transgenic Mouse Model for Echovirus Pathogenesis
    VINCENT RACANIELLO; Fiscal Year: 2004
    ..Establishment of a small animal model may yield information about echovirus pathogenesis that could stimulate development of echovirus vaccines or antiviral drugs. ..
  9. ROLES OF MICROFILAMENT PROTEINS IN NEOPLASIA/METASTASIS
    John Leavitt; Fiscal Year: 1993
    ....
  10. METALS EFFECT ON HEME METABOLISM
    MAHIN MAINES; Fiscal Year: 1999
    ..4) To investigate the role of HO isozymes in regulation in vivo and in vitro synthesis of NO and production of cGMP. ..
  11. DEMYELINATION IN MULTIPLE SCLEROSIS
    MOON SHIN; Fiscal Year: 1999
    ..Specifically, the role of C1 activation directly by myelin in vivo following breakdown of blood brain barrier will be examined as well as the role of TCC in inflammatory demyelination. ..
  12. Anti-Viral Gene Delivery in the Nervous System
    Daniel Carr; Fiscal Year: 2005
    ..abstract_text> ..
  13. Mammary Tumor Stem Cells
    Anne Peaston; Fiscal Year: 2009
    ..Extending the use of fluorescently marked PI-MEC to multiple mouse mammary tumor models will be important to understand the general extent to which this cell population contributes to different types of mammary cancer. ..
  14. PROCESSING OF EUKARYOTIC MESSENGER RNA
    Mary Edmonds; Fiscal Year: 1990
    ..If specific proteins are identified, their binding to the oligo U(+) mRNA population would be tested and ultimately binding to an oligo U containing mRNA such as beta-actin would be tested...
  15. Caveolin-1 and Cyclin D1 in Mammary Tumorigenesis
    Richard Pestell; Fiscal Year: 2006
    ..3). Determine if genetic ablation of the caveolin- 1 gene predisposes towards mammary tumorigenesis and metastasis in vivo. ..
  16. Effects of HMG-coA Reductase Pathway Inhibitors on GCAD
    William Stein; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  17. MECHANISM IN DOPAMINE RECEPTOR SENSITIVITY REGULATION
    JEANNETTE MILLER; Fiscal Year: 1990
    ....
  18. mRNA transport and local translation after axotomy
    Dianna Willis; Fiscal Year: 2007
    ..I will focus these analyses on axonal mRNAs encoding structural proteins (Beta-actin, peripherin and vimentin) and injury-response proteins (HSP70, grp78/BiP and Importin Beta1) that play central roles in axonal growth. ..
  19. MECHANISMS OF REGULATION OF CELL PROLIFERATION
    Michael Getz; Fiscal Year: 1993
    ....
  20. Spatial and temporal control of the mouse genome by lac
    Heidi Scrable; Fiscal Year: 2005
    ..Moreover, use of the lac system to regulate Hdh and Arc will allow us to explore the hypothesis that these genes play essential roles during synaptogenesis and in synaptic plasticity and spermatogenesis in the adult (aim 3) ..
  21. MPTP-Induced Homing of Bone Marrow Stem Cells to the Br*
    Lee Anna Cunningham; Fiscal Year: 2003
    ..If successful, these studies may provide the basis for future work to development new non-invasive gene therapies for Parkinson's disease, using accessible, renewable and autologous bone marrow stem cells. ..
  22. Neuronal mRNA transport & local translation analysis
    Jeffery Twiss; Fiscal Year: 2004
    ..g., spinal motor neurons) and whether we can utilize exogenous agents to increase local axonal protein synthesis for expediting axonal regeneration. ..
  23. ROLE OF METASCHEMATIN PROTEINS IN NEOPLASIA
    CHINGSHWUN LIN; Fiscal Year: 1991
    ..Finally, we will study the synergistic effect of metaschematin, plastin, and mutant beta-actin expression on the induction of the neoplastic phenotype in diploid human fibroblasts...
  24. Zebrafish Hair Cell Machines at Molecular Resolution
    Manfred Auer; Fiscal Year: 2005
    ..In addition, it will provide important insight into the pathogenesis of cytoplasmic actins and myosin-6-associated deafness. ..
  25. EF1ALPHA ACTIN IN CELL MOTILITY AND TRANSLATION
    John Condeelis; Fiscal Year: 2003
    ..mRNAs for beta-actin and Arp3, when localized to the leading edge, determine cell polarity. 2. The EF1a-F-actin complex is the scaffold to which mRNAs, with a beta-actin mRNA-like zipcode, become anchored. ..
  26. Transgenic Mouse Models for Pathogenesis and Therapy
    Tehsheng Chan; Fiscal Year: 2006
    ..The gene will be introduced under control of the general vector/promoter pCAGGS/chicken beta actin. Animals will be selected on the basis of the presence of the ACE2 gene, levels of protein expression, and early ..
  27. HIGH-THROUGHPUT SCREEN OF TOPICALLY APPLIED THERAPEUTICS
    Patrick Hayden; Fiscal Year: 2000
    ..The model will also be adaptable for other basic studies involving modulation of gene messages in skin by chemicals, UV radiation, or other exogenous treatments. ..
  28. Hippocampal GABAergic Neurons from Progenitor Grafts
    Vittorio Gallo; Fiscal Year: 2006
    ..Altogether, these studies will define a novel experimental paradigm aimed at repairing hippocampal GABAergic intemeuron damage in various pathological states, such as epilepsy, stroke, and ischemia ..
  29. Evolution of Promoter Activity in Conserved Genes
    GLENN MASTON; Fiscal Year: 2005
    ....
  30. MOLECULAR MECHANISMS WHEREBY CTG EXPANSION RESULTS IN DM
    Sita Reddy; Fiscal Year: 2004
    ..expanded CTG tracts integrated at random loci in the genome under the transcriptional control of the human beta actin promoter...
  31. MECHANISMS OF NO-MEDIATED PROTEIN S-NITROSYLATION IN EAE
    OSCAR BIZZOZERO; Fiscal Year: 2007
    ..abstract_text> ..
  32. NITRIC OXIDE, PLP ACYLATION & THE PATHOPHYSIOLOGY OF MS
    OSCAR BIZZOZERO; Fiscal Year: 2003
    ..abstract_text> ..
  33. Invasion of C. neoformans into brain endothelial cells
    Ambrose Jong; Fiscal Year: 2008
    ..The information derived from the studies is expected to be helpful in the development of novel strategies to prevent cryptococcal meningitis and its associated morbidity. ..
  34. REGULATION OF RENAL FUNCTION AND BP BY THROMBOXANE
    CHRISTOPHER WILCOX; Fiscal Year: 2002
    ....
  35. Cellular Immunology of Enterovirus Infection
    Paul Krogstad; Fiscal Year: 2007
    ..These studies will provide a foundation for further studies of host-pathogen relationships in picornavirus disease and the potential utility of picornaviruses as enterally delivered vaccine vectors. [unreadable] [unreadable] [unreadable]..
  36. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  37. HIV Replication and Thymopoiesis in Adolescents
    Paul Krogstad; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  38. Mutations in PKA gene transcripts of lupus T cells
    Dama Laxminarayana; Fiscal Year: 2007
    ..abstract_text> ..
  39. Individual Promoter SNP and CpG Methylation Signatures
    SIMON SPIVACK; Fiscal Year: 2006
    ..abstract_text> ..
  40. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  41. Innovative Investigations and Therapies for Asthma
    Richard Martin; Fiscal Year: 2007
    ..The ability to predict who will and will not respond to corticosteroids and additive therapy will greatly improve our ability to treat asthma effectively. ..
  42. Extreme Ammonia Tolerance Mechanisms: A Model Vertebrate
    Patrick Walsh; Fiscal Year: 2004
    ..abstract_text> ..
  43. MAP KINASE SIGNALING IN ASTROGLIAL REACTIONS
    James Mandell; Fiscal Year: 2003
    ..3) To generate transgenic mice expressing astrocyte-targeted activators of the ERK/MAPK pathway as critical tests of the working hypothesis. ..
  44. Restraint stress-induced neurogenic bladder inflammation
    Theoharis Theoharides; Fiscal Year: 2006
    ..Our findings may be relevant to the pathophysiology of IC and may suggest new therapeutic approaches. ..
  45. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  46. Conditional Deletion of Dnaic1 as a Model of Primary Ciliary Dyskinesia
    LAWRENCE OSTROWSKI; Fiscal Year: 2007
    ..If successful, this model will also provide a novel system to investigate other questions concerning pulmonary health and disease. [unreadable] [unreadable] [unreadable]..
  47. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. [unreadable]..
  48. High density mutagenesis of mtDNA
    Mikhail Alexeyev; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  49. Mechanism of Circadian Susceptibility to Pesticides
    LOUISA HOOVEN; Fiscal Year: 2008
    ..Humans are inevitably exposed to pyrethroids and other pesticides, and this interdisciplinary investigation is likely to lead to novel strategies protective of human health. [unreadable] [unreadable] [unreadable]..
  50. A Ciliated Cell-Specific Promoter for Gene Therapy of CF
    LAWRENCE OSTROWSKI; Fiscal Year: 2005
    ..Specific Aim 3: To demonstrate correction of the CF phenotype in both in vitro and in vivo models by targeted expression of the normal CFTR gene in ciliated cells. ..
  51. Design and Testing of a Parechovirus Vaccine Vector
    Paul Krogstad; Fiscal Year: 2005
    ..We propose to construct and complete the initial in vitro testing of a parechovirus based vaccine vector, based on strategies already employed in the successful construction of enteroviral vectors. ..
  52. Lesion Induced Synaptic Plasticity
    STEPHEN GINSBERG; Fiscal Year: 2004
    ....
  53. Varenicline and Smoking Cessation in Schizophrenia
    SEYYED HOSSEIN FATEMI; Fiscal Year: 2010
    ..Such outcomes will be significant, because they will offer a new treatment for smoking cessation in this vulnerable population. ..
  54. IL-1 Beta influences on NGF in neural Development
    ANNA JELASO; Fiscal Year: 2003
    ..This work will establish a system for understanding how cytokines and neurotrophins interact during neural development to influence the development and/or maintenance of functional neural circuits. ..
  55. TRANSGENIC STUDY VASCULAR ROLE OF NEUROPEPTIDE Y
    Michael Michalkiewicz; Fiscal Year: 2002
    ..The NPY overexpressing rats with well defined mutation of the NPY gene will provide a new transgenic animal model of hypertension; such a model should also be very useful for development of new therapies for treating this diseases. ..
  56. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  57. Role of Adenosine Receptors in Mast Cells
    Igor Feoktistov; Fiscal Year: 2008
    ..Finally, in Specific Aim 4, we will explore the relevance of adenosine receptor subtypes in an in vivo model of chronic pulmonary inflammation. [unreadable] [unreadable]..
  58. BIOMECHANICAL EFFECT OF ACUPUNCTURE NEEDLING
    HELENE LANGEVIN; Fiscal Year: 2001
    ..This will contribute to the fundamental understanding of acupuncture therapy and may lead to improvements in treatments. ..
  59. Role of Acetaldehyde in Alcoholic Cardiomyopathy
    Jun Ren; Fiscal Year: 2008
    ..Our long-term goal is to establish the toxic mechanism of acetaldehyde in the development of alcoholic cardiomyopathy so that prevention and treatment can be optimized [unreadable] [unreadable]..
  60. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  61. Prenatal Virally Induced Brain Disorder in Mouse
    SEYYED FATEMI; Fiscal Year: 2009
    ..In addition, it is expected that the results will provide clues that will lead to fundamental advances in our knowledge of the pathogenesis of schizophrenia and, therefore, of how it can be prevented and treated. ..
  62. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  63. MICROTUBULE/KERATIN INTERACTIONS DURING SPERMATOGENESIS
    ABRAHAM KIERSZENBAUM; Fiscal Year: 2002
    ..Engineering mouse models in which the expression of K9 is null, is important for both reproductive biology and cancer research. ..
  64. Retinal Circadian Signaling in a Transgenic Model
    Allan Wiechmann; Fiscal Year: 2004
    ..Discovering the molecular mechanisms of melatonin action may therefore provide a greater understanding of the role of circadian signals in environmentally- and genetically- induced photoreceptor degenerations. ..
  65. Prostate Cancer: A Mouse Model Testing 15-Lipoxygenase-1
    Uddhav Kelavkar; Fiscal Year: 2004
    ..abstract_text> ..
  66. Soft Tissue Biomechanical Behavior During Acupuncture in Low Back Pain
    Helene M Langevin; Fiscal Year: 2010
    ....
  67. Arsenic Embryotoxocity: Cellular and Molecular Targets
    M Pisano; Fiscal Year: 2003
    ....
  68. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  69. Arp2/3 mRNA localization and directional cell migration
    Gang Liu; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  70. The Role of Adenosine Receptors in Endothelial Cells
    Igor Feoktistov; Fiscal Year: 2005
    ..Our preliminary results also suggest that hypoxia modulates A2B receptor expression, a hypothesis that will be explored in Specific Aim IV. These experiments may lead to a novel approach for the treatment of pathologic angiogenesis. ..
  71. Regulation of Cx43 by Src and Novel Interacting Proteins
    Alan Lau; Fiscal Year: 2009
    ....
  72. CORTICOTROPIN RELEASING HORMONE INDUCED DURA MAST CELL A
    Theoharis Theoharides; Fiscal Year: 2001
    ..They may also help explain some key events in the pathophysiology of migraines, the clinical symptoms of which are often precipitated by stress. ..
  73. The Effect of Mycoplasma on Chronic Asthma
    Richard Martin; Fiscal Year: 2008
    ..pneumoniae and asthma will be obtained through this PPG and will ultimately give new knowledge to the understanding of asthma pathophysiology and potential future novel therapies. ..
  74. BIOLOGICAL PROPERTIES OF RPE MELANIN
    Janice M Burke; Fiscal Year: 2010
    ..The effects of age on melanosomes will also be studied to determine whether their ability to protect RPE cells from stress declines over time, which could contribute to diseases of the retina such as age-related macular degeneration. ..
  75. IDENTIFICATION OF BIOCHEMICAL ABNORMALITIES IN PCD CILIA
    LAWRENCE OSTROWSKI; Fiscal Year: 2002
    ..Further studies of the role of these proteins in the assembly and function of cilia may also result in improved treatment for other air way diseases. ..
  76. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  77. IGF-1, Oxidative Stress and Cardiovascular Aging
    Jun Ren; Fiscal Year: 2002
    ..Our long-term goal is to establish the causal link among IGF-1 deficiency, enhanced oxidative damage and ventricular dysfunction in the progression of cardiovascular aging so that hormonal or antioxidant therapy can be optimized...
  78. Hypoglycemic signaling targets in astrocytes
    James Mandell; Fiscal Year: 2003
    ..The longterm goal is to develop pharmacologic or molecular methods to augment or modulate neuroprotective aspects of the astrocytic response to hypoglycemia. ..
  79. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  80. High throughput PCR assays for diagnosing tuberculosis
    Sanjay Tyagi; Fiscal Year: 2007
    ..abstract_text> ..
  81. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  82. Determinants of Phenotype in Retinal Pigment Epithelium
    Janice Burke; Fiscal Year: 2007
    ..To determine whether the anticipated loss of polarity in RPE cells leads to a clinically or morphologically detectable retinal degeneration. ..
  83. Visualizing the Movement of mRNAs in Living Cells
    Sanjay Tyagi; Fiscal Year: 2007
    ..Through the development of these synergistic techniques, it will be possible to observe the movement of specific mRNAs in living cells. ..
  84. REGULATION AND FUNCTION OF LUNG SPECIFIC FREAC GENES
    Ye Shih Ho; Fiscal Year: 2002
    ..abstract_text> ..
  85. ASTHMA CLINICAL RESEARCH NETWORK (ACRN)
    Richard Martin; Fiscal Year: 2002
    ....
  86. Protein Biomarker Profiles of Abusive Alcohol Consumption
    Frank Witzmann; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  87. Synaptosomal Protein Changes After EtOH Self-Administration
    Frank Witzmann; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  88. ASTHMA CHRONOBIOLOGY: CENTRAL AND LUNG INTERACTIONS
    Richard Martin; Fiscal Year: 2003
    ....
  89. Molecular Diagnosis Utilizing Cerebrospinal Fluid
    STEPHEN GINSBERG; Fiscal Year: 2003
    ..abstract_text> ..
  90. THE PATHOBIOLOGY OF INHERITED RETINAL DEGENERATION
    Dean Bok; Fiscal Year: 2006
    ..abstract_text> ..
  91. Mast cells, antidepressants and chronic fatigue syndrome
    Theoharis Theoharides; Fiscal Year: 2007
    ..Future studies will build on these findings to develop in vitro and in vivo models of CFS and lead to clinical trials with select antidepressants or other molecules that inhibit brain mast cells. [unreadable] [unreadable] [unreadable]..
  92. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
  93. Functions of ERK and p38 MAP Kinases in Astrogliosis
    James Mandell; Fiscal Year: 2008
    ..This approach could promote recovery of brain and spinal cord function after injury or stroke. [unreadable] [unreadable]..
  94. SHEAR STRESS MODULATION OF ENDOTHELIAL WOUND HEALING
    MARIA LUIZA ALBUQUERQUE; Fiscal Year: 2003
    ..Investigation of the mechanisms underlying endothelial repair during shear stress may lead to better strategies for the management of patients suffering from a variety of cardiovascular disorders. ..
  95. ACTIN BASED MOTILITY BY RICKETTSIA RICKETTSII
    ROBERT HEINZEN; Fiscal Year: 2002
    ..Not only will these studies lead to a greater understanding of molecular mechanisms of rickettsial pathogenesis, but they will also provide insight into the dynamic control of actin polymerization. ..