ATG13

Summary

Gene Symbol: ATG13
Description: autophagy related 13
Alias: KIAA0652, PARATARG8, autophagy-related protein 13, ATG13 autophagy related 13 homolog
Species: human

Top Publications

  1. ncbi Prime-numbered Atg proteins act at the primary step in autophagy: unphosphorylatable Atg13 can induce autophagy without TOR inactivation
    Yoshiaki Kamada
    National Institute for Basic Biology, Okazaki, Japan
    Autophagy 6:415-6. 2010
  2. ncbi ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes
    Wiebe H Meijer
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Autophagy 3:106-16. 2007
  3. pmc Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 20:1981-91. 2009
  4. pmc ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery
    Chang Hwa Jung
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 20:1992-2003. 2009
  5. pmc ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy
    Ian G Ganley
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Biol Chem 284:12297-305. 2009
  6. pmc FIP200 regulates targeting of Atg16L1 to the isolation membrane
    Taki Nishimura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    EMBO Rep 14:284-91. 2013
  7. ncbi Nix, a receptor protein for mitophagy in mammals
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:433-5. 2010
  8. pmc ULK1 regulates melanin levels in MNT-1 cells independently of mTORC1
    Eyal Kalie
    Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, London, United Kingdom
    PLoS ONE 8:e75313. 2013
  9. doi A small switch has a large effect on autophagy
    Winfried Weissenhorn
    University Grenoble Alpes, 38000 Grenoble, France CNRS, UVHCI, 38000 Grenoble, France Unit of Virus Host Cell Interactions, University Grenoble Alpes EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France Electronic address
    Structure 22:1-2. 2014
  10. pmc Roles for PI(3,5)P2 in nutrient sensing through TORC1
    Natsuko Jin
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109 Department of Molecular, Cellular, and Developmental Biology and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 Department of Internal Medicine and Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 Department of Molecular Biology, University of Geneva, Geneva 1211, Switzerland Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109
    Mol Biol Cell 25:1171-85. 2014

Research Grants

  1. DEREK RONALD DUCKETT; Fiscal Year: 2014
  2. Role of Adipose Autophagy in Metabolism
    Do Hyung Kim; Fiscal Year: 2010
  3. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2013
  4. ULK, mediator of mTORC1 signaling to aging
    Do Hyung Kim; Fiscal Year: 2013
  5. Characterization of LC3-Associated Phagocytosis
    Jennifer Martinez; Fiscal Year: 2013
  6. Xuejun Jiang; Fiscal Year: 2016
  7. Cindy Puente; Fiscal Year: 2015
  8. Do Hyung Kim; Fiscal Year: 2016
  9. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007

Scientific Experts

  • Yoshiaki Kamada
  • Ioannis P Nezis
  • Justyna Gil
  • Piotr Czarny
  • Taeyoul Ha
  • Tomotake Kanki
  • Winfried Weissenhorn
  • K Muniyappa
  • Xin Yu
  • Kuninori Suzuki
  • Sebastian Alers
  • Claudine Kraft
  • MARTHA STIPANUK
  • Edmond Y Chan
  • Giichi Takaesu
  • Do Hyung Kim
  • Daniel J Klionsky
  • Chang Hwa Jung
  • Laurence Booth
  • James H Hurley
  • Michael J Ragusa
  • Noboru Mizushima
  • Thomas P Neufeld
  • Xuejun Jiang
  • Robin E Stanley
  • Yuh Ying Yeh
  • Cindy Puente
  • Jennifer Martinez
  • DEREK RONALD DUCKETT
  • Björn Stork
  • Christine C Jao
  • Antje S Löffler
  • Elaine A Dunlop
  • Takeshi Kaizuka
  • Fulvio Reggiori
  • Jennifer Jung
  • Paul Dent
  • Andrew Poklepovic
  • Jane L Roberts
  • Christian Behrends
  • Carlo Follo
  • Nao Hosokawa
  • Dario Barbone
  • Nora Hieke
  • Stefan Drießen
  • Nan Peng
  • Sebastian Wesselborg
  • Marine Maud Desgeorges
  • Vinay V Eapen
  • Ricardo C Ferreira
  • Heesun Cheong
  • Faqiang Li
  • Seung Hyun Ro
  • Jun Lin Guan
  • Mondira Kundu
  • E Tian
  • Paul K Herman
  • Yoshinori Ohsumi
  • Mirjana Sumarac-Dumanovic
  • Mehrad Tavallai
  • Weilin Liu
  • Qinwei Sun
  • Rachel Barrow-McGee
  • Nobuo N Noda
  • Shuang Zhao
  • V Martínez-Cisuelo
  • Yijian Rao
  • Zhong Hua Yan
  • Giulia Allavena
  • Paola G Bronson
  • Mario Mauthe
  • Shenglan Lin
  • V Courtney Broaddus
  • Raphael Bueno
  • Anne Simonsen
  • Christoph Peter
  • Huiwen Song
  • Jürgen Köfinger
  • Petter Holland
  • Fabian Stuhldreier
  • Christopher W Davies
  • Niklas Berleth
  • Olena Friesen
  • Ana Mesquita
  • Philip Böhler
  • Sho W Suzuki
  • Elizabeth Delorme-Axford
  • Sharon A Tooze
  • Natsuko Jin
  • Leonor Miller-Fleming

Detail Information

Publications108 found, 100 shown here

  1. ncbi Prime-numbered Atg proteins act at the primary step in autophagy: unphosphorylatable Atg13 can induce autophagy without TOR inactivation
    Yoshiaki Kamada
    National Institute for Basic Biology, Okazaki, Japan
    Autophagy 6:415-6. 2010
    ..The Atg1 kinase complex that comprises Atg1 and its binding prime-numbered Atg proteins (Atg11, Atg13, Atg17, Atg29 and Atg31) has long been a candidate for TORC1's downstream target...
  2. ncbi ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes
    Wiebe H Meijer
    Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    Autophagy 3:106-16. 2007
    ..This suggests that in fungi an organism-specific form of selective autophagy may occur, for which specialized Atg proteins have evolved...
  3. pmc Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 20:1981-91. 2009
    ..Here, we report a novel mammalian autophagy factor, Atg13, which forms a stable approximately 3-MDa protein complex with ULK1 and FIP200...
  4. pmc ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery
    Chang Hwa Jung
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 20:1992-2003. 2009
    ..Here, we identify that mTOR phosphorylates a mammalian homologue of Atg13 and the mammalian Atg1 homologues ULK1 and ULK2...
  5. pmc ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy
    Ian G Ganley
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Biol Chem 284:12297-305. 2009
    ..we report identification of a novel protein complex containing ULK1 and two additional protein factors, FIP200 and ATG13, all of which are essential for starvation-induced autophagy...
  6. pmc FIP200 regulates targeting of Atg16L1 to the isolation membrane
    Taki Nishimura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    EMBO Rep 14:284-91. 2013
    ..Thus, FIP200 regulates not only early events but also late events of autophagosome formation through direct interaction with Atg16L1...
  7. ncbi Nix, a receptor protein for mitophagy in mammals
    Tomotake Kanki
    Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Autophagy 6:433-5. 2010
    ..Recently, it was reported that Nix is a mitochondrial receptor that can directly connect to one of the autophagic machinery components, the Atg8 homologs LC3 and GABARAP...
  8. pmc ULK1 regulates melanin levels in MNT-1 cells independently of mTORC1
    Eyal Kalie
    Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, London, United Kingdom
    PLoS ONE 8:e75313. 2013
    ..evidence to show that ULK1 function in this context is independent of the canonical ULK1 autophagy partners, ATG13 and FIP200. Furthermore we show that regulation of melanogenesis by ULK1 is independent of mTORC1 inhibition...
  9. doi A small switch has a large effect on autophagy
    Winfried Weissenhorn
    University Grenoble Alpes, 38000 Grenoble, France CNRS, UVHCI, 38000 Grenoble, France Unit of Virus Host Cell Interactions, University Grenoble Alpes EMBL CNRS, 6 rue Jules Horowitz, 38042 Grenoble, France Electronic address
    Structure 22:1-2. 2014
    Atg8 family proteins and Atg13 are important regulators of autophagy...
  10. pmc Roles for PI(3,5)P2 in nutrient sensing through TORC1
    Natsuko Jin
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109 Department of Molecular, Cellular, and Developmental Biology and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109 Department of Internal Medicine and Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109 Department of Molecular Biology, University of Geneva, Geneva 1211, Switzerland Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109
    Mol Biol Cell 25:1171-85. 2014
    ..is required for multiple downstream pathways via TORC1-dependent phosphorylation of additional targets, including Atg13, the modification of which inhibits autophagy, and phosphorylation of Npr1, which releases its inhibitory function ..
  11. pmc Yeast DJ-1 superfamily members are required for diauxic-shift reprogramming and cell survival in stationary phase
    Leonor Miller-Fleming
    Instituto de Medicina Molecular, 1649 028 Lisbon, Portugal Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom
    Proc Natl Acad Sci U S A 111:7012-7. 2014
    ..disrupted target of rapamycin complex 1 (TORC1) localization to P-bodies, and caused abnormal TORC1-mediated Atg13 phosphorylation...
  12. doi The HORMA domain: an evolutionarily conserved domain discovered in chromatin-associated proteins, has unanticipated diverse functions
    K Muniyappa
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India Electronic address
    Gene 545:194-7. 2014
    ..Another recent study reveals that S. cerevisiae Atg13 HORMA domain acts as a phosphorylation-dependent conformational switch in the cellular autophagic process.
  13. pmc Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells
    Di Ge
    Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology School of Life Science Shandong University Jinan, China
    Autophagy 10:957-71. 2014
    ..Further experiments results showed that FLJ11812 could bind with MIR4459 targeting ATG13 (autophagy-related 13), and ATG13 protein level was decreased along with 3BDO-decreased FLJ11812 level...
  14. pmc An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E⁻/⁻ mice
    Nan Peng
    1 Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China 2
    Sci Rep 4:5519. 2014
    ..results showed that oxLDL inhibited the activity of mTOR and increased the protein level of autophagy-related 13 (ATG13) and its dephosphorylation, thus inducing autophagy in human umbilical vein endothelial cells (HUVECs)...
  15. doi Post-transcriptional regulation of autophagy in C2C12 myotubes following starvation and nutrient restoration
    Marine Maud Desgeorges
    Laboratoire de Physiologie de l Exercice EA 4338, Universite de Lyon, Université Jean Monnet, Saint Etienne, France
    Int J Biochem Cell Biol 54:208-16. 2014
    ..LC3B-II level and LC3B-positive punctate), and an increase in the content of autophagy-related proteins (Ulk1, Atg13, Vps34, and Atg5-Atg12 conjugate)...
  16. pmc FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation
    Elaine A Dunlop
    Institute of Cancer and Genetics, Cardiff University Heath Park, Cardiff, Wales UK
    Autophagy 10:1749-60. 2014
    ..Consequently, this work reveals a novel facet of autophagy regulation by ULK1 and substantially contributes to our understanding of FLCN function by linking it directly to autophagy through GABARAP and ULK1. ..
  17. pmc How the Atg1 complex assembles to initiate autophagy
    Christopher W Davies
    a Department of Molecular and Cell Biology and California Institute for Quantitative Biosciences, University of California, Berkeley Berkeley, CA USA
    Autophagy 11:185-6. 2015
    The Atg1 complex, comprising Atg1, Atg13, Atg17, Atg29, and Atg31, is a key initiator of autophagy...
  18. pmc Atg13 HORMA domain recruits Atg9 vesicles during autophagosome formation
    Sho W Suzuki
    Frontier Research Center and Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226 8501, Japan and
    Proc Natl Acad Sci U S A 112:3350-5. 2015
    ..b>Atg13, which plays a central role in the initial step of PAS formation, consists of two structural regions, the N-..
  19. pmc Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site
    Jürgen Köfinger
    Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max von Laue Straße 3, 60438 Frankfurt am Main, Germany Electronic address
    Structure 23:809-18. 2015
    ..The Atg1 complex consists of the Atg1 protein kinase, the intrinsically disordered region-rich Atg13, and the dimeric double crescent-shaped Atg17-Atg31-Atg29 subcomplex...
  20. pmc ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation
    Huiwen Song
    a Department of Cardiology Affiliated Baoan Hospital of Southern Medical University Shenzhen, China
    Autophagy 11:1308-25. 2015
    Recent studies have shown that the phosphorylation and dephosphorylation of ULK1 and ATG13 are related to autophagy activity...
  21. pmc Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy
    Stefan Drießen
    a Institute of Molecular Medicine Heinrich Heine University Düsseldorf, Germany
    Autophagy 11:1458-70. 2015
    ..The ULK1 kinase complex consisting of the serine/threonine protein kinase ULK1 and the adapter proteins ATG13, RB1CC1, and ATG101, is centrally involved in the regulation of autophagy initiation...
  22. pmc Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells
    Nora Hieke
    a Institute of Molecular Medicine I Heinrich Heine University Düsseldorf, Germany
    Autophagy 11:1471-83. 2015
    ..The ULK1/2 complex comprising the kinase ULK1/2 and the accessory proteins ATG13, RB1CC1, and ATG101 has been identified as a central player in the autophagy network, and it represents the main ..
  23. pmc Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase
    Ana Mesquita
    Instituto de Investigaciones Biom dicas Alberto Sols, CSIC UAM, Madrid, Spain
    Open Biol 5:. 2015
    ..discoideum is more similar to mammalian cells than to Saccharomyces cerevisiae as, besides Atg13, it contains Atg101, a protein not conserved in this yeast...
  24. pmc Autophagy Correlates with the Therapeutic Responsiveness of Malignant Pleural Mesothelioma in 3D Models
    Dario Barbone
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, California, 94110, United States of America
    PLoS ONE 10:e0134825. 2015
    ..Instead, sensitivity to GDC-0980 correlated with the presence of constitutive ATG13 puncta, a feature of autophagy, a cellular program that supports cells under stress...
  25. pmc A missing piece of the puzzle: Atg11 functions as a scaffold to activate Atg1 for selective autophagy
    Elizabeth Delorme-Axford
    a Life Sciences Institute University of Michigan Ann Arbor, MI USA
    Autophagy 11:2139-41. 2015
    ..starvation or rapamycin treatment repress TOR complex 1 and stimulate the Atg1 complex (including at least Atg1, Atg13, Atg17, Atg29 and Atg31), which allows the recruitment of downstream autophagy-related (Atg) components to the ..
  26. pmc Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway
    Weilin Liu
    College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P R China
    Int J Mol Med 37:309-18. 2016
    ..protein 1 light chain 3 beta (LC3B)Ⅱ/Ⅰ, Unc-51-like kinase 1 (ULK1), autophagy related gene 13 Atg13) and Beclin1 (ser14) were decreased, whereas mTORC1 expression was increased in the peri-infarct cortex...
  27. pmc The Atg1-kinase complex tethers Atg9-vesicles to initiate autophagy
    Yijian Rao
    Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany
    Nat Commun 7:10338. 2016
    ..This interaction is inhibited by the two regulatory subunits Atg31 and Atg29. Engagement of the Atg1-Atg13 subcomplex restores the Atg9-binding and membrane-tethering activity of Atg17...
  28. doi Atg101: Not Just an Accessory Subunit in the Autophagy-initiation Complex
    Nobuo N Noda
    Laboratory of Structural Chemistry and Biology, Institute of Microbial Chemistry BIKAKEN
    Cell Struct Funct 41:13-20. 2016
    The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), ..
  29. pmc The afatinib resistance of in vivo generated H1975 lung cancer cell clones is mediated by SRC/ERBB3/c-KIT/c-MET compensatory survival signaling
    Laurence Booth
    Departments of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Oncotarget 7:19620-30. 2016
    ..treatment profoundly inactivated ERBB3, AKT and mTOR in the H1975 afatinib resistant clones and increased ATG13 S318 phosphorylation...
  30. pmc Autophagy initiation correlates with the autophagic flux in 3D models of mesothelioma and with patient outcome
    Carlo Follo
    a San Francisco General Hospital, University of California San Francisco, San Francisco, CA, USA
    Autophagy 12:1180-94. 2016
    ..by detection of the accumulation of LC3 after lysosomal inhibition and as autophagy initiation by detection of ATG13 puncta. We found that autophagic flux in 3D, but not in 2D, correlated with ATG13 positivity...
  31. pmc HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation
    Shenglan Lin
    Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China
    Oxid Med Cell Longev 2016:2586706. 2016
    ..Furthermore, old Tg hearts demonstrated lower accumulation of LC3-II and p62 than old WT hearts. Levels of Atg13, Vps34, and Rab7 were also higher in old Tg hearts than in old WT hearts...
  32. doi Folate deprivation modulates the expression of autophagy- and circadian-related genes in HT-22 hippocampal neuron cells through GR-mediated pathway
    Qinwei Sun
    Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China
    Steroids 112:12-9. 2016
    ..05) up-regulated in FD group, which includes ATG2b, ATG3, ATG4c, ATG5, ATG10, ATG12, ATG13 and ATG14...
  33. pmc Multi-kinase inhibitors interact with sildenafil and ERBB1/2/4 inhibitors to kill tumor cells in vitro and in vivo
    Laurence Booth
    Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298, USA
    Oncotarget 7:40398-40417. 2016
    ..fashion to: (i) profoundly activate an eIF2α/Beclin1 pathway; (ii) profoundly inactivate mTOR and increase ATG13 phosphorylation, collectively resulting in the formation of toxic autophagosomes...
  34. pmc Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes
    Rachel Barrow-McGee
    Spatial Signalling Team, Centre for Tumour Biology, Barts Cancer Institute A Cancer Research UK Centre of Excellence, Queen Mary University of London, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, UK
    Nat Commun 7:11942. 2016
    ..This signalling is dependent on ATG5 and Beclin1 but not on ATG13, suggesting ARE belong to a non-canonical autophagy pathway...
  35. doi An ultrasensitive assay format for detecting ULK1 inhibition by monitoring the phosphorylation status of Atg13
    Zhong Hua Yan
    Takeda Pharmaceuticals International Co, Cambridge, MA, USA
    Anal Biochem 509:73-8. 2016
    ..Our studies focused upon the inhibition of the autophagy initiating kinase ULK1 by measuring the both total Atg13 and the phosphorylation of Atg13(pSer(318)) from control and following compound treatment in either overexpressing ..
  36. pmc The down-regulated ING5 expression in lung cancer: a potential target of gene therapy
    Shuang Zhao
    Cancer Center, Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, and Animal Center, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121001, China
    Oncotarget 7:54596-54615. 2016
    ..ING5 transfection up-regulated the expression of Cdc2, ATG13, ATG14, Beclin-1, LC-3B, AIF, cytochrome c, Akt1/2/3, ADFP, PFK-1 and PDPc, while down-regulated the expression of ..
  37. doi Rapamycin reverses age-related increases in mitochondrial ROS production at complex I, oxidative stress, accumulation of mtDNA fragments inside nuclear DNA, and lipofuscin level, and increases autophagy, in the liver of middle-aged mice
    V Martínez-Cisuelo
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, E 28040, Spain Electronic address
    Exp Gerontol 83:130-8. 2016
    ..Rapamycin also decreased the amount of RAPTOR (of mTOR complex) and increased the amounts of the PGC1-α and ATG13 proteins...
  38. pmc An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication
    Mario Mauthe
    Department of Cell Biology, University Medical Center Groningen, University of Groningen, 9713 AV Groningen, Netherlands Department of Cell Biology, University Medical Center Utrecht, 3584 CX Utrecht, Netherlands
    J Cell Biol 214:619-35. 2016
    ..Detailed analysis of two candidates revealed an undocumented role for ATG13 and FIP200 in picornavirus replication that is independent of their function in autophagy as part of the ULK ..
  39. pmc Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation
    Giulia Allavena
    a Institute of Environmental Medicine, Division of Toxicology, Karolinska Institutet, Stockholm, Sweden
    Autophagy 12:2085-2097. 2016
    ..These inhibitors could also drastically increase the ULK1 protein in U1810 cells with knockout of ATG13, where the ULK1 expression is significantly diminished...
  40. pmc Common variants at PVT1, ATG13-AMBRA1, AHI1 and CLEC16A are associated with selective IgA deficiency
    Paola G Bronson
    Department of Human Genetics, Genentech, Inc, South San Francisco, California, USA
    Nat Genet 48:1425-1429. 2016
    ..Ile923Val). Peak new variants (PVT1, P = 4.3 × 10-11; ATG13-AMBRA1, P = 6.7 × 10-10; AHI1, P = 8.4 × 10-10; CLEC16A, P = 1...
  41. doi Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome
    Mirjana Sumarac-Dumanovic
    Clinic for Endocrinology, Diabetes, and Diseases of Metabolism, Clinical Center of Serbia, Belgrade, Serbia School of Medicine, University of Belgrade, Belgrade, Serbia
    Mol Cell Endocrinol 440:116-124. 2017
    ..of transcription factor forkhead box protein O1 (FOXO1) and several molecules involved in autophagosome formation (ATG13, RB1-inducible coiled-coil 1), autophagosome nucleation (ATG14, beclin 1, SH3-domain GRB2-like endophilin B1), ..
  42. pmc PDE5 inhibitors enhance the lethality of pemetrexed through inhibition of multiple chaperone proteins and via the actions of cyclic GMP and nitric oxide
    Laurence Booth
    Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298 0035, USA
    Oncotarget 8:1449-1468. 2017
    ..pathway that was associated with reduced mTOR S2448 and ULK-1 S757 phosphorylation and increased ULK-1 S317 and ATG13 S318 phosphorylation...
  43. pmc The BAX gene as a candidate for negative autophagy-related genes regulator on mRNA levels in colorectal cancer
    Justyna Gil
    Department of Genetics, Wroclaw Medical University, 50 368, Wroclaw, Poland
    Med Oncol 34:16. 2017
    ..Probe Library was used to assess the expression of the seven following selected genes: BECN1, UVRAG, ULK1, ATG13, Bif-1, BCL2 and BAX. For all but one of the tested genes, a decrease in expression was observed...
  44. pmc Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator
    Jennifer Jung
    Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany
    elife 6:. 2017
    ..Global mRNA expression analysis revealed that SMCR8 regulates transcription of several other autophagy genes including WIPI2. Collectively, we established SMCR8 as multifaceted negative autophagy regulator...
  45. pmc The beginning of the end: how scaffolds nucleate autophagosome biogenesis
    Robin E Stanley
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 24:73-81. 2014
    ..A multiprotein complex comprising the protein kinase autophagy-related protein 1 (Atg1) together with Atg13, Atg17, Atg29, and Atg31 (ULK1, ATG13, FIP200, and ATG101 in humans) has a pivotal role in the earliest steps of ..
  46. pmc Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae
    Shenshen Zou
    College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China
    Traffic 14:233-46. 2013
    ..Genetic studies showed that the effect of the Trs130 mutation was downstream of Atg5 and upstream of Atg1, Atg13, Atg9 and Atg14 on the autophagic pathway...
  47. pmc DNA damage signaling triggers the cytoplasm-to-vacuole pathway of autophagy to regulate cell cycle progression
    Vinay V Eapen
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA, USA
    Autophagy 9:440-1. 2013
    ..GARP genes can be mimicked by hyperactivation of the Cvt pathway by overexpressing an unphosphorylatable form of ATG13 or by adding the TORC1 inhibitor rapamycin...
  48. pmc CCCP-Induced LC3 lipidation depends on Atg9 whereas FIP200/Atg13 and Beclin 1/Atg14 are dispensable
    Daohong Chen
    Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, United States
    Biochem Biophys Res Commun 432:226-30. 2013
    ..In addition, deletion or knockdown of the UKC component FIP200 or Atg13 only led to a partial reduction in LC3 lipidation, indicating that UKC could be also dispensable for this step ..
  49. pmc A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy
    Christine C Jao
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 110:5486-91. 2013
    Autophagy-related 13 (Atg13) is a key early-acting factor in autophagy and the major locus for nutrient-dependent regulation of autophagy by Tor. The 2...
  50. doi Fine mapping of autophagy-related proteins during autophagosome formation in Saccharomyces cerevisiae
    Kuninori Suzuki
    BioImaging Center, Graduate School of Frontier Sciences, University of Tokyo, FSB 101, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    J Cell Sci 126:2534-44. 2013
    ..The PAS scaffold proteins (Atg13 and Atg17) and phosphatidylinositol 3-kinase complex I were localized to a position at the junction between the IM ..
  51. doi Inhibition of autophagy via p53-mediated disruption of ULK1 in a SCA7 polyglutamine disease model
    Xin Yu
    Department of Neurochemistry, Stockholm University, 106 91, Stockholm, Sweden
    J Mol Neurosci 50:586-99. 2013
    ..Together, this leads to a decreased capacity for autophagy induction via the ULK1-FIP200-Atg13-Atg101 complex...
  52. pmc Anthricin Isolated from Anthriscus sylvestris (L.) Hoffm. Inhibits the Growth of Breast Cancer Cells by Inhibiting Akt/mTOR Signaling, and Its Apoptotic Effects Are Enhanced by Autophagy Inhibition
    Chang Hwa Jung
    Department of Food Biotechnology, University of Science and Technology, Daejon 305 806, Republic of Korea Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201 Anyangpangyo ro, Seongnam 463 746, Republic of Korea
    Evid Based Complement Alternat Med 2013:385219. 2013
    ..Interestingly, blockage of autophagy by a pharmacological inhibitor or genetic deletion of ULK1 and Atg13 accelerated anthricin-induced apoptosis, suggesting that autophagy has cytoprotective effects...
  53. doi Regulation of Akt-mTOR, ubiquitin-proteasome and autophagy-lysosome pathways in response to formoterol administration in rat skeletal muscle
    Olivier Roger Joassard
    Laboratoire de Physiologie de l Exercice, Universite de Lyon, F 42023 Saint Etienne, France
    Int J Biochem Cell Biol 45:2444-55. 2013
    ..E1B interacting protein3 (Bnip3), and transcription factor EB (TFEB), as well as the protein content of Ulk1, Atg13, Atg5-Atg12 complex and p62/Sqstm1 remained unchanged or was even decreased in response to formoterol ..
  54. doi Dynamic association of the ULK1 complex with omegasomes during autophagy induction
    Eleftherios Karanasios
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB22 3AT, UK
    J Cell Sci 126:5224-38. 2013
    ..Here we show that the ULK1 complex colocalises with omegasomes in a PtdIns3P-dependent way. Live-cell imaging of Atg13 (a ULK1 complex component), omegasomes and LC3 establishes and annotates for the first time a complete sequence of ..
  55. pmc Distinct functions of Ulk1 and Ulk2 in the regulation of lipid metabolism in adipocytes
    Seung Hyun Ro
    Department of Biochemistry, Molecular Biology and Biophysics University of Minnesota Minneapolis, MN USA
    Autophagy 9:2103-14. 2013
    ..However, unlike other autophagy genes (Atg5, Atg13, Rb1cc1/Fip200, and Becn1) Ulk1 was dispensable for adipogenesis without affecting the expression of CCAAT/..
  56. pmc ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding
    Elaine A Dunlop
    Institute of Medical Genetics, Cardiff University, Cardiff, UK
    Autophagy 7:737-47. 2011
    ..This is a novel negative feedback loop that occurs upon activation of autophagy to maintain mTORC1 inhibition when nutrient supplies are limiting...
  57. pmc DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase
    Farokh Dotiwala
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02445, USA
    Proc Natl Acad Sci U S A 110:E41-9. 2013
    ..Autophagy in response to DNA damage can be induced in three different ways: by deleting the Golgi-associated retrograde protein complex (GARP), by adding rapamycin, or by overexpression of a dominant ATG13-8SA mutation.
  58. pmc Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis
    Michael J Ragusa
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 151:1501-12. 2012
    ..Upon induction of autophagy, Atg17-Atg31-Atg29 assembles with Atg1 and Atg13, which in turn initiates the formation of the phagophore...
  59. ncbi Inhibition of autophagy enhances sunitinib-induced cytotoxicity in rat pheochromocytoma PC12 cells
    Tatsuhiko Ikeda
    Department of Endocrine Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
    J Pharmacol Sci 121:67-73. 2013
    ..Indeed, direct mTORC1 inhibition initiates ULK1/2 autophosphorylation and subsequent Atg13 and FIP200 phosphorylation, inducing autophagy...
  60. pmc What the N-terminal domain of Atg13 looks like and what it does: a HORMA fold required for PtdIns 3-kinase recruitment
    Christine C Jao
    Laboratory of Molecular Biology National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, MD USA
    Autophagy 9:1112-4. 2013
    b>Atg13 is a subunit of the Atg1 complex that is involved in autophagy. The middle and C-terminal regions of Atg13 are intrinsically disordered and rich in regulatory phosphorylation sites...
  61. doi Knockdown of mTOR by lentivirus‑mediated RNA interference suppresses atherosclerosis and stabilizes plaques via a decrease of macrophages by autophagy in apolipoprotein E‑deficient mice
    XiaoChuang Wang
    Department of Emergency Medicine, the Second Affiliated Hospital of Medical College, Xi an Jiaotong University, Xi an, Shaanxi 710004, P R China
    Int J Mol Med 32:1215-21. 2013
    ..In addition, western blot analysis revealed that the knockdown of mTOR increased autophagy‑related protein 13 (Atg13) dephosphorylation and light chain 3‑I/light chain 3‑II (LC3‑I/LC3‑II) ratios, both of which were ..
  62. doi TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy
    Giichi Takaesu
    Center for Integrated Medical Research, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku ku, Tokyo, 160 8582 Japan
    J Biochem 151:157-66. 2012
    ..Here we report that TAB2 and TAB3 bind to Beclin1 and colocalize in the cytoplasm. TAB2 also interacts with ATG13 and is phosphorylated by ULK1...
  63. ncbi Atg13 and FIP200 act independently of Ulk1 and Ulk2 in autophagy induction
    Sebastian Alers
    Department of Internal Medicine I University Clinic of Tübingen Tübingen, Germany
    Autophagy 7:1423-33. 2011
    ..of Unc-51-like kinases 1/2 (Ulk1/2), focal adhesion kinase family-interacting protein of 200 kDa (FIP200) and Atg13. Upon starvation, mTORC1-mediated repression of this complex is released, which then leads to Ulk1/2 activation...
  64. pmc Pro-tumorigenic function of autophagy in mammary oncogenesis
    Huijun Wei
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Autophagy 8:129-31. 2012
    ..FIP200 (focal adhesion kinase family interacting protein of 200 kD) is a component of the ULK1-Atg13-FIP200-Atg101 complex that is essential for the induction of mammalian autophagy...
  65. pmc The incredible ULKs
    Sebastian Alers
    Department of Internal Medicine I, University Hospital of Tubingen, Otfried M and 252ller Str, 10, 72076 Tubingen, Germany
    Cell Commun Signal 10:7. 2012
    ..This review will mainly focus on the vertebrate Ulk1/2-Atg13-FIP200 protein complex, its function in autophagy initiation, its evolutionary descent from the yeast Atg1-Atg13-..
  66. pmc Ksp1 kinase regulates autophagy via the target of rapamycin complex 1 (TORC1) pathway
    Midori Umekawa
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    J Biol Chem 287:16300-10. 2012
    ..kinase functions in part as a negative regulator of autophagy; deletion of KSP1 facilitated dephosphorylation of Atg13, a TORC1 substrate, which correlates with enhanced autophagy...
  67. pmc Regulator and substrate: dual roles for the ATG1-ATG13 kinase complex during autophagic recycling in Arabidopsis
    Faqiang Li
    Department of Genetics, University of Wisconsin Madison, Madison, WI, USA
    Autophagy 8:982-4. 2012
    ..The process is tightly regulated by the autophagy-related 1(ATG1)-ATG13 kinase complex which is controlled by multiple nutrient-responsive upstream regulators that integrate nutrient ..
  68. pmc The protein factor-arrest 11 (Far11) is essential for the toxicity of human caspase-10 in yeast and participates in the regulation of autophagy and the DNA damage signaling
    Patricia Lisa-Santamaría
    Instituto de Biología Funcional y Genómica and Departamento de Microbiología y Genética, CSIC Universidad de Salamanca, Campus Miguel de Unamuno, E 37007 Salamanca, Spain
    J Biol Chem 287:29636-47. 2012
    ..Furthermore, the protein Far11, which is a target of MAP kinases, is essential for the dephosphorylation of Atg13 and, consequently, for the induction of autophagy...
  69. pmc Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy
    Claudine Kraft
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 31:3691-703. 2012
    ..The Atg1/ULK1 kinase is an essential component of the core autophagy machinery possibly activated by binding to Atg13 upon starvation...
  70. doi The C. elegans ATG101 homolog EPG-9 directly interacts with EPG-1/Atg13 and is essential for autophagy
    Qianqian Liang
    College of Life Sciences, Beijing Normal University, Beijing, China
    Autophagy 8:1426-33. 2012
    ..exhibit autophagy phenotypes characteristic of those associated with loss of function of unc-51/Atg1 and epg-1/Atg13. epg-9 encodes a protein with significant homology to mammalian ATG101. EPG-9 directly interacts with EPG-1/Atg13...
  71. pmc ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs
    Endalkachew Ashenafi Alemu
    Molecular Cancer Research Group, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 287:39275-90. 2012
    ..The mammalian ULK complex involved in autophagy consists of ULK1, ULK2, ATG13, FIP200, and ATG101...
  72. ncbi The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure
    Fulvio Reggiori
    Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Dev Cell 6:79-90. 2004
    ..We show that Atg9 and Atg23 cycle through the PAS in a process governed by the Atg1-Atg13 signaling complex...
  73. pmc Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy
    Yukiko Kabeya
    Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 16:2544-53. 2005
    ..Two-hybrid analyses and coimmunoprecipitation experiments demonstrated that Atg17 physically associates with Atg1-Atg13 complex, and this binding was enhanced under starvation conditions...
  74. pmc Atg17 regulates the magnitude of the autophagic response
    Heesun Cheong
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 16:3438-53. 2005
    ..As a result, it is defective in peroxisome degradation and is partially defective for autophagy. Atg17 interacts with both Atg1 and Atg13, via two coiled-coil domains, and these interactions facilitate its inclusion in the Atg1 complex.
  75. pmc The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae
    Heesun Cheong
    Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:668-81. 2008
    ..The Atg1 C terminus mediates an interaction with Atg13 and Atg17, facilitating a structural role of Atg1 that is needed to efficiently organize an initial step of PAS ..
  76. pmc Organization of the pre-autophagosomal structure responsible for autophagosome formation
    Tomoko Kawamata
    Department of Cell Biology, National Institute for Basic Biology, and School of Life Science, The Graduate University for Advanced Studies, Okazaki 444 8585, Japan
    Mol Biol Cell 19:2039-50. 2008
    ..The Atg1 kinase complex (Atg1-Atg13) is also essential for recruitment of Atg proteins to the PAS...
  77. ncbi Dual role of Atg1 in regulation of autophagy-specific PAS assembly in Saccharomyces cerevisiae
    Hessun Cheong
    Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:724-6. 2008
    ..We found that Atg1, Atg13 and Atg17 play a similar role for PAS formation under autophagy-inducing conditions as seen for Atg11 during ..
  78. pmc Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism
    Edmond Y W Chan
    Secretory Pathways Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Mol Cell Biol 29:157-71. 2009
    ..other distinct regions that direct membrane association and interaction with the putative human homologue of Atg13, which we have here characterized. Atg13 is required for autophagy and Atg9 trafficking during autophagy...
  79. pmc An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation
    Yu Yun Chang
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Biol Cell 20:2004-14. 2009
    ..Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila...
  80. ncbi A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy
    Carol A Mercer
    Department of Cancer and Cell Biology, Genome Research Institute, University of Cincinnati, Cincinnati, OH, USA
    Autophagy 5:649-62. 2009
    ..In yeast, the Atg1 kinase complex includes Atg1, Atg13, Atg17, and at least four other interacting proteins, some of which are phosphorylated in a TOR-dependent manner, ..
  81. ncbi epg-1 functions in autophagy-regulated processes and may encode a highly divergent Atg13 homolog in C. elegans
    E Tian
    College of Life Sciences, China Agricultural University, Beijing, China
    Autophagy 5:608-15. 2009
    ..epg-1 encodes a novel protein that shows limited sequence similarity to the yeast autophagy protein Atg13. epg-1 displays a similar expression pattern to, and directly interacts with, the C...
  82. ncbi Atg101, a novel mammalian autophagy protein interacting with Atg13
    Nao Hosokawa
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    Autophagy 5:973-9. 2009
    ..In yeast, Atg1 forms a complex with Atg11, Atg13, Atg17, Atg29 and Atg31, whereas mammalian Atg1 (ULK1/2) interacts with Atg13 and FIP200...
  83. doi mTORC1 phosphorylates the ULK1-mAtg13-FIP200 autophagy regulatory complex
    Edmond Y Chan
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
    Sci Signal 2:pe51. 2009
    ..mechanism in which mTORC1 phosphorylates the autophagy regulatory complex containing unc-51-like kinase 1 (ULK1), the mammalian Atg13 protein, and focal adhesion kinase interacting protein of 200 kD (FIP200) has also been elucidated.
  84. pmc The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy
    Joseph S Stephan
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 106:17049-54. 2009
    ..We show that in addition to Atg1, PKA directly phosphorylates Atg13, a conserved regulator of Atg1 kinase activity...
  85. doi Macroautophagy and its role in nutrient homeostasis
    Martha H Stipanuk
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    Nutr Rev 67:677-89. 2009
    ..In addition, the recent placement of the Atg1*Atg13*Atg17 complex downstream of mTORC1 has provided at least one pathway by which changes in nutrient availability ..
  86. pmc Tor directly controls the Atg1 kinase complex to regulate autophagy
    Yoshiaki Kamada
    Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Cell Biol 30:1049-58. 2010
    ..However, the mechanism by which TORC1 controls autophagy and the direct target of TORC1 activity remain unclear. Atg13 is an essential regulatory component of autophagy upstream of the Atg1 kinase complex, and here we show that yeast ..
  87. pmc Autophosphorylation within the Atg1 activation loop is required for both kinase activity and the induction of autophagy in Saccharomyces cerevisiae
    Yuh Ying Yeh
    Department of Molecular Genetics, The Ohio State University, Columbus Ohio 43210, USA
    Genetics 185:871-82. 2010
    ..required the presence of a functional Atg1 kinase domain and two known regulators of Atg1 activity, Atg13 and Atg17...
  88. doi DAP1, a novel substrate of mTOR, negatively regulates autophagy
    Itay Koren
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Biol 20:1093-8. 2010
    ..Thus far, only a few substrates of mTOR that control autophagy have been identified, including ULK1 and Atg13, both of which function as positive mediators...
  89. ncbi "Autophagy suite": Atg9 cycling in the cytoplasm to vacuole targeting pathway
    Nobuo Munakata
    Rikkyo University, Faculty of Science, Biophysics Laboratory, Tokyo, Japan
    Autophagy 6:679-85. 2010
    ..Additional proteins, including Atg11, Atg23 and Atg27 are involved in this anterograde movement, whereas Atg1-Atg13 and Atg2-Atg18 are required for the retrograde return to the peripheral sites...
  90. doi Association of IFIH1 and other autoimmunity risk alleles with selective IgA deficiency
    Ricardo C Ferreira
    Genentech, Inc, South San Francisco, California, USA
    Nat Genet 42:777-80. 2010
    ..0 x 10(-4)) or random SNPs across the genome (P < 0.0001). These findings support the hypothesis that autoimmune mechanisms may contribute to the pathogenesis of IgAD...
  91. pmc Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    J Cell Biol 190:523-31. 2010
    ..Inhibition of autophagy by genetically removing the function of the autophagy genes atg1, atg13, and vps34 resulted in late stage egg chambers that contained persisting nurse cell nuclei without fragmented DNA ..
  92. pmc An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
    Muriel Mari
    Department of Cell Biology and Institute of Biomembranes, University Medical Centre Utrecht, Utrecht University, 3584 CX Utrecht, The Netherlands
    J Cell Biol 190:1005-22. 2010
    ..In addition, genetic analyses indicate that Atg1, Atg13, and phosphatidylinositol-3-phosphate are involved in the further rearrangement of these initial membranes...
  93. pmc Autophagy as a trigger for cell death: autophagic degradation of inhibitor of apoptosis dBruce controls DNA fragmentation during late oogenesis in Drosophila
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    Autophagy 6:1214-5. 2010
    ..Genetic inhibition: of autophagy-related genes atg1, atg13 and vps34 results in late-stage egg chambers containing persisting nurse cell nuclei without fragmented DNA and ..
  94. ncbi Activation of Atg1 kinase in autophagy by regulated phosphorylation
    Monika Kijanska
    Institute of Biochemistry, Department of Biology, ETH Züric, Zurich, Switzerland
    Autophagy 6:1168-78. 2010
    ..assembly site (PAS), or the proper assembly of the multisubunit Atg1 kinase complex and binding to its activator Atg13. However, mutation of either one of these sites results in a loss of Atg1 kinase activity and its function in ..
  95. ncbi FIP200, an essential component of mammalian autophagy is indispensible for fetal hematopoiesis
    Fei Liu
    Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Autophagy 7:229-30. 2011
    ..family interacting protein of 200 kD) is a newly identified essential autophagy gene and a component of the ULK-Atg13-FIP200 complex...
  96. ncbi Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop
    Antje S Löffler
    Department of Internal Medicine I, University of Tubingen, Tubingen, Germany
    Autophagy 7:696-706. 2011
    Unc-51-like kinase 1 (Ulk1) plays a central role in autophagy induction. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)...
  97. pmc Mitochondria regulate autophagy by conserved signalling pathways
    Martin Graef
    Department of Molecular and Cellular Biology, Davis University of California, Davis, CA, USA
    EMBO J 30:2101-14. 2011
    ..respiratory deficiency suppresses autophagic flux, autophagy gene induction, and recruitment of the Atg1-Atg13 kinase complex to the pre-autophagosomal structure by stimulating PKA activity...
  98. pmc Upregulation of human autophagy-initiation kinase ULK1 by tumor suppressor p53 contributes to DNA-damage-induced cell death
    W Gao
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell Death Differ 18:1598-607. 2011
    In yeast, activation of ATG1/ATG13 kinase complex initiates autophagy...
  99. ncbi Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae
    Chun Shik Shin
    School of Biological Sciences and Research Center for Functional Cellulomics, Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
    Autophagy 7:854-62. 2011
    ..In addition, we found that Atg13 is gradually rephosphorylated during prolonged nitrogen starvation, and the kinase activity of Atg1 is required ..
  100. doi Paraproteins of familial MGUS/multiple myeloma target family-typical antigens: hyperphosphorylation of autoantigens is a consistent finding in familial and sporadic MGUS/MM
    Sandra Grass
    José Carreras Center for Immuno and Gene Therapy, Department Internal Medicine I, Saarland University Medical School, Homburg Saar, Germany
    Blood 118:635-7. 2011
    ..targeted P-7, and paraproteins from 4 affected members of a fourth family targeted P-8, which is encoded by the ATG13 gene...
  101. pmc An Atg13 protein-mediated self-association of the Atg1 protein kinase is important for the induction of autophagy
    Yuh Ying Yeh
    Department of Molecular Genetics, Ohio State University, Columbus Ohio 43210, USA
    J Biol Chem 286:28931-9. 2011
    ..In this study we show that Atg13, an evolutionarily conserved regulator of Atg1, promotes the formation of a specific Atg1 self-interaction in the ..

Research Grants9

  1. DEREK RONALD DUCKETT; Fiscal Year: 2014
    ..Multi-PI research team has shown that Ulk1 kinase activity is essential for control of the autophagy pathway that Atg13 is a bona fide substrate of Ulk1 and that Ulk1-directed phosphorylation of Atg13 on S318 is essential for ..
  2. Role of Adipose Autophagy in Metabolism
    Do Hyung Kim; Fiscal Year: 2010
    ..The central hypothesis is that mTOR-regulated protein complex, which consists of ULK1 (Unc51-like protein 1) and Atg13 (mammalian homolog of yeast AuToPhagy gene 13), plays a crucial role in the regulation of the induction of ..
  3. Regulation of TOR Signaling and Autophagy in Drosophila
    Thomas P Neufeld; Fiscal Year: 2013
    ..the activity of a multicomponent complex containing the autophagy related kinase Atg1 and the phosphoprotein Atg13. Specific aims of this project are to: 1) test potential models of Atg1/13 complex regulation, including the ..
  4. ULK, mediator of mTORC1 signaling to aging
    Do Hyung Kim; Fiscal Year: 2013
    ..hypothesis has been formulated based on our preliminary data demonstrating protein complexes containing ULK and Atg13. We identified these protein complexes as targets of mTORC1 and the mediators of mTORC1 activity toward the ..
  5. Characterization of LC3-Associated Phagocytosis
    Jennifer Martinez; Fiscal Year: 2013
    ..These studies will include examination of the ULK1-ATG13-FIP200 complex, mTOR, and the Beclin1-VPS34- ATG14L-UVRAG complex, all important mediators of the autophagic ..
  6. Xuejun Jiang; Fiscal Year: 2016
    ..and is autophagy pathway a potential target for cancer therapy? Recently, we and others identified the ULK1-ATG13-FIP200 complex as an essential component of mammalian autophagy that mediates the activity of the nutrient-sensing ..
  7. Cindy Puente; Fiscal Year: 2015
    ..Molecularly, many autophagy-related (Atg) proteins are required for autophagy, with the Ulk1-Atg13- FIP200 complex functioning at the top of the hierarchy...
  8. Do Hyung Kim; Fiscal Year: 2016
    ..Recent studies from my group revealed that mTORC1, the ULK1-Atg13-FIP200-Atg101 complex, and the Beclin 1-PI3KC3 (hVps34)-Atg14L complex interact and form a large macromolecular ..
  9. Nutritional Regulation of y-Glutamylcysteine Synthetase
    MARTHA STIPANUK; Fiscal Year: 2007
    ..Capacity for GSH synthesis, availability of the precursor amino acid cysteine, and steady-state GSH levels all contribute to the body's ability to defend itself against oxidative or chemical stressors. ..