aquaporin 4

Summary

Gene Symbol: aquaporin 4
Description: aquaporin 4
Alias: MIWC, WCH4, aquaporin-4, aquaporin type4, mercurial-insensitive water channel
Species: human

Top Publications

  1. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
  2. ncbi Aquaporin-1, nothing but a water channel
    Satoshi P Tsunoda
    Forschungsinstitut fuer Molekulare Pharmakologie, Robert Roessle Strasse 10, D 13125 Berlin, Germany
    J Biol Chem 279:11364-7. 2004
  3. pmc Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance
    Joseph D Ho
    Graduate Program in Chemistry and Chemical Biology and Department of Biochemistry and Biophysics, Genentech Hall, University of California, 600 16th Street, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 106:7437-42. 2009
  4. ncbi Fetal development of membrane water channel proteins aquaporin-1 and aquaporin-4 in the human brain
    Eva Gomori
    Department of Pathology, Faculty of Medicine, University of Pecs, 7643 Pecs, Szigeti u 12, Hungary
    Int J Dev Neurosci 24:295-305. 2006
  5. doi Variants of the genes encoding AQP4 and Kir4.1 are associated with subgroups of patients with temporal lobe epilepsy
    Kjell Heuser
    Department of Neurology, Rikshospitalet University Hospital, Oslo, Norway
    Epilepsy Res 88:55-64. 2010
  6. doi The water permeability channels aquaporins 1-4 are differentially expressed in granulosa and theca cells of the preovulatory follicle during precise stages of human ovulation
    Asgeir Thoroddsen
    Department of Obstetrics and Gynaecology, Sahlgrenska Academy, University of Gothenburg, SE 413 45 Goteborg, Sweden
    J Clin Endocrinol Metab 96:1021-8. 2011
  7. ncbi Aquaporin 1 and aquaporin 4 expression in human brain after subarachnoid hemorrhage and in peritumoral tissue
    J Badaut
    Department of Neurosurgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Acta Neurochir Suppl 86:495-8. 2003
  8. ncbi Increased expression of water channel aquaporin 1 and aquaporin 4 in Creutzfeldt-Jakob disease and in bovine spongiform encephalopathy-infected bovine-PrP transgenic mice
    Agustin Rodriguez
    Institut de Neuropatologia, Servei Anatomia Patològica, IDIBELL Hospital Universitari de Bellvitge, Universitat de Barcelona, Hospitalet de Llobregat, Barcelona, Spain
    Acta Neuropathol 112:573-85. 2006
  9. ncbi Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein
    G M Preston
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Science 256:385-7. 1992
  10. pmc Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance
    J S Jung
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Proc Natl Acad Sci U S A 91:13052-6. 1994

Research Grants

  1. Alan S Verkman; Fiscal Year: 2016
  2. HANS CHRISTIAN VON BUEDINGEN; Fiscal Year: 2015
  3. Jeffrey L Bennett; Fiscal Year: 2016
  4. Michael Levy; Fiscal Year: 2016
  5. SEAN JOSEPH PITTOCK; Fiscal Year: 2014
  6. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    CHRISTINA ROSE MARMAROU; Fiscal Year: 2012
  7. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    Anthony Marmarou; Fiscal Year: 2007
  8. NFkB in the Astrocyte Swelling/Brain Edema with Acute Liver Failure
    MICHAEL DAVID NORENBERG; Fiscal Year: 2012
  9. MICHAEL DAVID NORENBERG; Fiscal Year: 2016
  10. AQP4 and JNK Inhibition Together Reduce Edema and Excitotoxic Injury in jTBI
    Jerome Badaut; Fiscal Year: 2013

Detail Information

Publications265 found, 100 shown here

  1. ncbi Structural determinants of water permeation through aquaporin-1
    K Murata
    National Institute for Physiological Sciences, Okazaki, Japan
    Nature 407:599-605. 2000
    ..The atomic model provides a possible molecular explanation to a longstanding puzzle in physiology-how membranes can be freely permeable to water but impermeable to protons...
  2. ncbi Aquaporin-1, nothing but a water channel
    Satoshi P Tsunoda
    Forschungsinstitut fuer Molekulare Pharmakologie, Robert Roessle Strasse 10, D 13125 Berlin, Germany
    J Biol Chem 279:11364-7. 2004
    ..The lack of significant CNG ion channel activity rules out a secondary role of AQP1 water channels in cellular signal transduction...
  3. pmc Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance
    Joseph D Ho
    Graduate Program in Chemistry and Chemical Biology and Department of Biochemistry and Biophysics, Genentech Hall, University of California, 600 16th Street, San Francisco, CA 94158 2517, USA
    Proc Natl Acad Sci U S A 106:7437-42. 2009
    ....
  4. ncbi Fetal development of membrane water channel proteins aquaporin-1 and aquaporin-4 in the human brain
    Eva Gomori
    Department of Pathology, Faculty of Medicine, University of Pecs, 7643 Pecs, Szigeti u 12, Hungary
    Int J Dev Neurosci 24:295-305. 2006
    ..Neuronal labeling was not observed. These results in human fetal brain lend morphological support to the previous findings that aquaporin-1 and aquaporin-4 play different roles in the regulation of the water homeostasis of the brain...
  5. doi Variants of the genes encoding AQP4 and Kir4.1 are associated with subgroups of patients with temporal lobe epilepsy
    Kjell Heuser
    Department of Neurology, Rikshospitalet University Hospital, Oslo, Norway
    Epilepsy Res 88:55-64. 2010
    ..The current study was designed to identify variants of the AQP4 and KCNJ10 genes associated with TLE and subgroups of this condition...
  6. doi The water permeability channels aquaporins 1-4 are differentially expressed in granulosa and theca cells of the preovulatory follicle during precise stages of human ovulation
    Asgeir Thoroddsen
    Department of Obstetrics and Gynaecology, Sahlgrenska Academy, University of Gothenburg, SE 413 45 Goteborg, Sweden
    J Clin Endocrinol Metab 96:1021-8. 2011
    ..Changes in vascular permeability and expansion of the fluid-filled antrum are major events in the LH-induced ovulatory process...
  7. ncbi Aquaporin 1 and aquaporin 4 expression in human brain after subarachnoid hemorrhage and in peritumoral tissue
    J Badaut
    Department of Neurosurgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    Acta Neurochir Suppl 86:495-8. 2003
    ..These results reinforce the hypothesis that AQPs may be involved in the dynamics of brain edema formation or resolution. Further studies are needed to understand their functional role...
  8. ncbi Increased expression of water channel aquaporin 1 and aquaporin 4 in Creutzfeldt-Jakob disease and in bovine spongiform encephalopathy-infected bovine-PrP transgenic mice
    Agustin Rodriguez
    Institut de Neuropatologia, Servei Anatomia Patològica, IDIBELL Hospital Universitari de Bellvitge, Universitat de Barcelona, Hospitalet de Llobregat, Barcelona, Spain
    Acta Neuropathol 112:573-85. 2006
    ..by gel electrophoresis and Western blotting, the expression levels of the water channels aquaporin 1 (AQP1) and aquaporin 4 (AQP4) in the frontal cortex (area 8) homogenates of sporadic CJD cases (six men, four women; seven cases with ..
  9. ncbi Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein
    G M Preston
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Science 256:385-7. 1992
    ..Therefore it is likely that CHIP28 is a functional unit of membrane water channels...
  10. pmc Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance
    J S Jung
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205
    Proc Natl Acad Sci U S A 91:13052-6. 1994
    ....
  11. ncbi The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 271:4577-80. 1996
    The mercurial insensitive water channel (MIWC, AQP-4) is a water-selective transporter expressed at the basolateral plasma membrane of principal cells in kidney collecting duct, airway epithelium, and gastric parietal cells, as well as ..
  12. pmc The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain
    M Lu
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    Proc Natl Acad Sci U S A 93:10908-12. 1996
    ..2 and q12.1 by fluorescence in situ hybridization. These studies may now permit molecular characterization of AQP4 during human development and in clinical disorders...
  13. pmc Aquaporin-6: An intracellular vesicle water channel protein in renal epithelia
    M Yasui
    Departments of Biological Chemistry and Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205 2185, USA
    Proc Natl Acad Sci U S A 96:5808-13. 1999
    ..Moreover, our studies predict that AQP6 participates in distinct physiological functions such as glomerular filtration, tubular endocytosis, and acid-base metabolism...
  14. ncbi Reduced aquaporin 4 expression in the muscle plasma membrane of patients with Duchenne muscular dystrophy
    Yoshihiro Wakayama
    Department of Neurology, Showa University Fujigaoka Hospital, 1 30 Fujigaoka, Aoba ku, Yokohama 227 8501, Japan
    Arch Neurol 59:431-7. 2002
    ..Recent investigations showed that orthogonal arrays were composed of aquaporin 4 (AQP4) molecules, a member of the water channel protein family.
  15. ncbi New possible roles for aquaporin-4 in astrocytes: cell cytoskeleton and functional relationship with connexin43
    Grazia P Nicchia
    Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics CEGBA, University of Bari, Bari, Italy
    FASEB J 19:1674-6. 2005
    ..Finally, the strong down-regulation of Cx43 and cell coupling in AQP4 KD mouse astrocytes indicate that a functional relationship likely exists between water channels and gap junctions in brain astrocytes...
  16. ncbi Loss of aquaporin-4 in active perivascular lesions in neuromyelitis optica: a case report
    Tatsuro Misu
    Department of Neurology, Tohoku University School of Medicine, Sendai, Japan
    Tohoku J Exp Med 209:269-75. 2006
    ....
  17. ncbi Neuromyelitis optica brain lesions localized at sites of high aquaporin 4 expression
    Sean J Pittock
    Department of Neurology, Mayo Clinic College of Medicine, Rochester, MN 55905 0001, USA
    Arch Neurol 63:964-8. 2006
    Neuromyelitis optica (NMO)-IgG is a specific autoantibody marker for NMO. It binds selectively to aquaporin 4 (AQP4), which is highly concentrated in astrocytic foot processes at the blood-brain barrier and is not restricted to optic ..
  18. ncbi Expression pattern of the water channel aquaporin-4 in human gliomas is associated with blood-brain barrier disturbance but not with patient survival
    Arne Warth
    Institute of Pathology, University of Tuebingen, Tuebingen, Germany
    J Neurosci Res 85:1336-46. 2007
    ..In addition, our results provide unexpectedly high AQP4 levels in pilocytic astrocytomas and present AQP4 as tumor progression marker in WHO grade II-IV astrocytomas...
  19. pmc Expression and function of water channels (aquaporins) in migrating malignant astrocytes
    Eric McCoy
    Department of Neurobiology, Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Glia 55:1034-43. 2007
    ..Yet, only the presence of AQP1, but not AQP4, enhanced cell growth and migration, typical properties of gliomas, while AQP4 enhanced cell adhesion suggesting differential biological roles for AQP1 and AQP4 in glioma cell biology...
  20. doi Differential expression of aquaporin-4 in human gastric normal and cancer tissues
    H Xu
    Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, PR China
    Gastroenterol Clin Biol 33:72-6. 2009
    ..Aquaporin-4 (AQP4) is a member of the water channel protein family, which is thought to be associated with tumor development. A recent work suggests that AQP play key roles in tumor biology...
  21. pmc Intrathecal pathogenic anti-aquaporin-4 antibodies in early neuromyelitis optica
    Jeffrey L Bennett
    Department of Neurology and Ophthalmology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA
    Ann Neurol 66:617-29. 2009
    ..Our objectives were to determine the source of central nervous system (CNS) NMO-IgGs and their role in disease pathogenesis...
  22. pmc Evidences for a leaky scanning mechanism for the synthesis of the shorter M23 protein isoform of aquaporin-4: implication in orthogonal array formation and neuromyelitis optica antibody interaction
    Andrea Rossi
    Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics, University of Bari, Bari 70125, Italy
    J Biol Chem 285:4562-9. 2010
    ..In this study we also provide evidence that AQP4-M1 is mobile in the plasma membrane, that it is inserted and not excluded into immobile OAPs, and that it is an important determinant of OAP structure and size...
  23. pmc Patterns of antibody binding to aquaporin-4 isoforms in neuromyelitis optica
    Simone Mader
    Clinical Department of Neurology, Innsbruck Medical University, Innsbruck, Austria
    PLoS ONE 5:e10455. 2010
    ..Serum NMO-IgG autoantibodies (Abs), a specific finding in NMO patients, target the water channel protein aquaporin-4 (AQP4), which is expressed as a long (M-1) or a short (M-23) isoform...
  24. doi Aquaporin-4 astrocytopathy in Baló's disease
    Takeshi Matsuoka
    Department of Neuropathology, Kyushu University, Higashi ku, Fukuoka, Japan
    Acta Neuropathol 120:651-60. 2010
    ..Furthermore, acute BCS lesions are characterized by extensive AQP4 loss without vasculocentric deposition of complement or immunoglobulin...
  25. doi Aquaporin-4 in glioma invasion and an analysis of molecular mechanisms
    Ting Ding
    Department of Breast Pathology, Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education, Hexi District, Tianjin, China
    J Clin Neurosci 17:1359-61. 2010
    ..This review focuses on the newly discovered functions and molecular mechanisms of AQP4 in infiltrative gliomas...
  26. pmc Identification of two major conformational aquaporin-4 epitopes for neuromyelitis optica autoantibody binding
    Francesco Pisani
    Department of General and Environmental Physiology, Centre of Excellence in Comparative Genomics, University of Bari, I 70126 Bari, Italy
    J Biol Chem 286:9216-24. 2011
    ....
  27. pmc Binding affinity and specificity of neuromyelitis optica autoantibodies to aquaporin-4 M1/M23 isoforms and orthogonal arrays
    Jonathan M Crane
    Department of Medicine, University of California, San Francisco, California 94143, USA
    J Biol Chem 286:16516-24. 2011
    ..Our study establishes a quantitative assay of NMO-IgG binding to AQP4 and indicates remarkable, OAP-dependent heterogeneity in NMO autoantibody binding specificity...
  28. pmc Aquaporin-4 Mz isoform: brain expression, supramolecular assembly and neuromyelitis optica antibody binding
    Andrea Rossi
    Department of Medicine, University of California, San Francisco, California 94143 0521, USA
    Glia 59:1056-63. 2011
    ....
  29. doi Role of aquaporins in cell migration and edema formation in human brain tumors
    Beatrice Nico
    Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy
    Exp Cell Res 317:2391-6. 2011
    ..This review article summarizes literature data concerning the involvement of AQP-1 and -4 in human brain tumor growth and edema formation and suggests a potential therapeutic approach by antagonizing their biological activity...
  30. doi Quantitative measurement of anti-aquaporin-4 antibodies by enzyme-linked immunosorbent assay using purified recombinant human aquaporin-4
    Woojun Kim
    Department of Neurology, Research Institute and Hospital of National Cancer Center, Goyang, Korea
    Mult Scler 18:578-86. 2012
    ..Antibodies to aquaporin-4 (AQP4-Ab), known as NMO-IgG, are a sensitive and specific marker for neuromyelitis optica (NMO)...
  31. ncbi Aquaporin 1 and aquaporin 4 are involved in invasion of lung cancer cells
    Yanping Xie
    Department of Respiratory, First People s Hospital of Huzhou, Zhejiang Province, China
    Clin Lab 58:75-80. 2012
    ..Most recently, overexpression of several AQPs has been reported in different types of human cancer, which indicates that AQPs may play an important role in human carcinogenesis...
  32. doi Quantitative assays for anti-aquaporin-4 antibody with subclass analysis in neuromyelitis optica
    Noriko Isobe
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Japan
    Mult Scler 18:1541-51. 2012
    ..To clarify the clinical relevance of anti-aquaporin-4 (anti-AQP4) antibody titers and immunoglobulin (IgG) subclass...
  33. pmc Astrocytic autoantibody of neuromyelitis optica (NMO-IgG) binds to aquaporin-4 extracellular loops, monomers, tetramers and high order arrays
    Raffaele Iorio
    Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street S W, Rochester, MN 55905, USA
    J Autoimmun 40:21-7. 2013
    ..The relative abundance and localization of AQP4 high order arrays in distinct CNS regions may explain the variability in clinical phenotype of NMO spectrum disorders...
  34. ncbi A water channel closely related to rat brain aquaporin 4 is expressed in acid- and pepsinogen-secretory cells of human stomach
    T Misaka
    Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Japan
    FEBS Lett 381:208-12. 1996
    ..The protein was designated human aquaporin 4 (hAQP4) because of its 94% sequence similarity to rat brain AQP4...
  35. ncbi Expression and localization of epithelial aquaporins in the adult human lung
    S M Kreda
    Cystic Fibrosis Pulmonary Research and Clinical Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    Am J Respir Cell Mol Biol 24:224-34. 2001
    ..This study describes an intricate network of AQP expression that mediates water transport across the human airway epithelium...
  36. pmc Syntrophin-dependent expression and localization of Aquaporin-4 water channel protein
    J D Neely
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 98:14108-13. 2001
    ..These results demonstrate that perivascular localization of AQP4 in brain requires alpha-Syn, and stability of AQP4 in the membrane is increased by the C-terminal PDZ-binding motif...
  37. ncbi Reduced expression of aquaporin 4 in human muscles with amyotrophic lateral sclerosis and other neurogenic atrophies
    Takahiro Jimi
    Department of Neurology, Showa University Fujigaoka Hospital, 1 30 Fujigaoka, Aoba ku, Yokohama 227 8501, Japan
    Pathol Res Pract 200:203-9. 2004
    b>Aquaporin 4 (AQP4) is a water channel protein that is widely distributed in human tissues. However, the precise functional role of AQP4 in skeletal muscle tissue has not yet been determined...
  38. ncbi Linkage disequilibrium in aquaporin 4 gene and association study with schizophrenia
    Tatsuyuki Muratake
    Department of Psychiatry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
    Psychiatry Clin Neurosci 59:595-8. 2005
    b>Aquaporin 4 (AQP4) has an important role in water homeostasis of human brain and a dysfunction of AQP4 could induce pathological conditions in neuronal activity...
  39. ncbi Human astrocytes express aquaporin-1 and aquaporin-4 in vitro and in vivo
    Jun ichi Satoh
    Department of Bioinformatics, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Tokyo 204 8588, Japan
    Neuropathology 27:245-56. 2007
    ....
  40. ncbi Aquaporin-4, homeostasis, and neurologic disease
    Eduardo E Benarroch
    Mayo Clinic, 200 First Street SW, West 8A Mayo Bldg or 811 Guggenheim Bldg, Rochester, MN 55905, USA
    Neurology 69:2266-8. 2007
  41. doi The role of aquaporin-4 polymorphisms in the development of brain edema after middle cerebral artery occlusion
    Ilka Kleffner
    Stroke 39:1333-5. 2008
    ..We asked whether genetic variation in the AQP4 gene is related to the severity of brain edema after middle cerebral artery occlusion...
  42. doi Changes in skeletal muscle expression of AQP1 and AQP4 in dystrophinopathy and dysferlinopathy patients
    Carol G Au
    Kids Heart Research, The Children s Hospital at Westmead, Discipline of Paediatrics and Child Health, Faculty of Medicine, University of Sydney, Locked Bag 4001, Westmead, Sydney, NSW, 2145, Australia
    Acta Neuropathol 116:235-46. 2008
    ..Our study shows that AQP4 downregulation can occur in muscular dystrophies with either normal or disrupted expression of dystrophin-associated proteins, and that this might be associated with upregulation of AQP1...
  43. pmc Neuromyelitis optica pathogenesis and aquaporin 4
    David J Graber
    Department of Pathology, Dartmouth Medical School, Lebanon, New Hampshire, USA
    J Neuroinflammation 5:22. 2008
    ..An IgG1 autoantibody (NMO-IgG) that binds aquaporin 4 (AQP4) has been identified in the sera of a significant number of NMO patients, as well as in patients with two ..
  44. pmc Novel variants in human Aquaporin-4 reduce cellular water permeability
    MARCO D SORANI
    Program in Biological and Medical Informatics, University of California, San Francisco, San Francisco, CA 94110, USA
    Hum Mol Genet 17:2379-89. 2008
    ..Our experiments provide insight into the function of the AQP4 protein. These nsSNPs may have clinical implications for patients with cerebral edema and related disorders...
  45. ncbi Aquaporin-4 expression is severely reduced in human sarcoglycanopathies and dysferlinopathies
    Stefania Assereto
    Muscular and Neurodegenerative Disease Unit, Department of Pediatrics of University of Genova, Department of Neuroscience and Rehabilitation, G Gaslini Institute, Genoa, Italy
    Cell Cycle 7:2199-207. 2008
    ..In conclusion, AQP4 expression and membrane localization are markedly reduced in LGMD 2B-2F. The role of AQP4 in the degenerative mechanism occurring in these diseases will be the object of our future research...
  46. doi Aquaporin-4 expression is increased in edematous meningiomas
    Wai Hoe Ng
    Department of Neurosurgery, National Neuroscience Institute, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Level 3, Clinical Staff Office, 308433 Singapore
    J Clin Neurosci 16:441-3. 2009
    ..The inhibition of AQP4 water channels is a potential therapeutic option to reduce the adverse effects of peritumoral edema in meningiomas...
  47. doi Diagnosis of neuromyelitis spectrum disorders: comparative sensitivities and specificities of immunohistochemical and immunoprecipitation assays
    Andrew McKeon
    Department of Neurology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Arch Neurol 66:1134-8. 2009
    ....
  48. pmc Water permeability through aquaporin-4 is regulated by protein kinase C and becomes rate-limiting for glioma invasion
    E S McCoy
    Department of Neurobiology and Center for Glial Biology in Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Neuroscience 168:971-81. 2010
    ..AQP4 expressing glioma cells showed significantly reduced invasion compared to AQP1 and S180 expressing tumors as determined by quantitative stereology, consistent with a differential role for AQP1 and AQP4 in this process...
  49. ncbi [AQP4 expression in the brains of patients with glioblastoma and its association with brain edema]
    Ke Jie Mou
    Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China
    Sichuan Da Xue Xue Bao Yi Xue Ban 40:651-4. 2009
    ..To investigate the expression of aquaporin-4 (AQP4) in the brains of patients with glioblastoma and its association with brain edema...
  50. pmc MicroRNA 320a functions as a novel endogenous modulator of aquaporins 1 and 4 as well as a potential therapeutic target in cerebral ischemia
    Sugunavathi Sepramaniam
    Department of Biochemistry, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore
    J Biol Chem 285:29223-30. 2010
    ..We have also shown that anti-miR-320a could bring about a reduction of infarct volume in cerebral ischemia with a concomitant increase in aquaporins 1 and 4 mRNA and protein expression...
  51. doi Role of aquaporin-4 in the regulation of migration and invasion of human glioma cells
    Ting Ding
    Department of Breast Pathology, Tianjin Medical University, Cancer Institute and Hospital, Tianjin 300060, PR China
    Int J Oncol 38:1521-31. 2011
    ..In summary, our results indicate that AQP4 is involved in the control of glioblastoma cell migration and invasion and may be a potential therapeutic target for glioblastoma cell infiltration...
  52. doi Detection of anti-aquaporin-4 antibodies in neuromyelitis optica: comparison of tissue-based and cell-based indirect immunofluorescence assays and ELISA
    Yoon Joo Kim
    Department of Laboratory Medicine, School of Medicine, The Catholic University of Korea, Seoul, Korea
    J Clin Lab Anal 26:184-9. 2012
    ..01). In summary, we observed an increased prevalence of NMO-IgG in patients with NMO and NMO spectrum disorders. ELISA-AQP4 may be more sensitive and specific when confirmed by IIF-AQP4...
  53. pmc Intra-cerebral injection of neuromyelitis optica immunoglobulin G and human complement produces neuromyelitis optica lesions in mice
    Samira Saadoun
    St George s, University of London, London SW17 0RE, UK
    Brain 133:349-61. 2010
    ..In our mouse model, immunoglobulin G from neuromyelitis optica patients does not require pre-existing central nervous system inflammation to produce lesions...
  54. doi Propofol inhibits aquaporin 4 expression through a protein kinase C-dependent pathway in an astrocyte model of cerebral ischemia/reoxygenation
    Sheng Mei Zhu
    Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hang Zhou 310003, People s Republic of China
    Anesth Analg 109:1493-9. 2009
    b>Aquaporin 4 (AQP4) plays a key role in maintaining water balance in the central nervous system, and its dysfunction may lead to brain edema...
  55. ncbi Altered expression of renal AQPs and Na(+) transporters in rats with lithium-induced NDI
    T H Kwon
    Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000 Aarhus C, United Kingdom
    Am J Physiol Renal Physiol 279:F552-64. 2000
    ..In contrast, the increased or unchanged expression of NHE3, BSC-1, Na-K-ATPase, and TSC indicates that these Na(+) transporters do not participate in the development of Li-induced polyuria...
  56. ncbi A prominent elevation of glial fibrillary acidic protein in the cerebrospinal fluid during relapse in neuromyelitis optica
    Rina Takano
    Department of Neurology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Tohoku J Exp Med 215:55-9. 2008
    ..CSF-GFAP may be useful as a biomarker of NMO...
  57. ncbi Downregulation of renal aquaporins in response to unilateral ureteral obstruction
    Chunling Li
    The Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus C, Denmark
    Am J Physiol Renal Physiol 284:F1066-79. 2003
    ....
  58. ncbi Lead induces increased water permeability in astrocytes expressing aquaporin 4
    E Gunnarson
    Nordic Centre for Water Imbalance Related Disorders, Department of Woman and Child Health, Karolinska Institutet, Pediatric Unit, Research Laboratory, Q2 09 Astrid Lindgren Children s Hospital, 171 76 Stockholm, Sweden
    Neuroscience 136:105-14. 2005
    The water channel aquaporin 4 (AQP4) is abundantly expressed in astrocytes. There is now compelling evidence that AQP4 may contribute to an unfavorable course in brain edema...
  59. ncbi Severe alterations of endothelial and glial cells in the blood-brain barrier of dystrophic mdx mice
    Beatrice Nico
    Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy
    Glia 42:235-51. 2003
    ..The BBB alterations suggest that changes in vascular permeability are involved in the pathogenesis of the neurological dysfunction associated with DMD...
  60. doi Aquaporin-4 antibodies in neuromyelitis optica and longitudinally extensive transverse myelitis
    Patrick Waters
    Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, England
    Arch Neurol 65:913-9. 2008
    ..There is increasing recognition of antibody-mediated immunotherapy-responsive neurologic diseases and a need for appropriate immunoassays...
  61. ncbi Heterogeneity of aquaporin-4 autoimmunity and spinal cord lesions in multiple sclerosis in Japanese
    Takeshi Matsuoka
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashiku, Fukuoka 812 8582, Japan
    Brain 130:1206-23. 2007
    ..This indicated that the mechanisms producing LESCLs are also heterogeneous in cases with optic-spinal presentation, namely AQP4 autoimmunity-related and -unrelated...
  62. ncbi [Recent advances of multiple sclerosis research in Japan]
    Jun Ichi Kira
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University
    Rinsho Shinkeigaku 49:549-59. 2009
    ..These findings indicate that both anti-AQP4 autoimmunity-related and -unrelated OSMS occur in Japanese. Th17/Th1 cells are involved in both conditions, while additional humoral factors also act in the former...
  63. doi AQP-4 in peritumoral edematous tissue is correlated with the degree of glioma and with expression of VEGF and HIF-alpha
    Kejie Mou
    West China Hospital, Sichuan University, Chengdu, Sichuan, China
    J Neurooncol 100:375-83. 2010
    ....
  64. ncbi Establishment of a new sensitive assay for anti-human aquaporin-4 antibody in neuromyelitis optica
    Toshiyuki Takahashi
    Department of Neurology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Tohoku J Exp Med 210:307-13. 2006
    ..Thus, the newly developed anti-AQP4 antibody assay appears to have a higher sensitivity for NMO than the original NMO-IgG assay and is expected to be useful for the diagnosis of NMO...
  65. doi Brain edema after intracerebral hemorrhage in rats: the role of iron overload and aquaporin 4
    Wang Gai Qing
    Department of Neurology, Second Xiangya Hospital of Central South University, Tai Yuan, Shan Xi, China
    J Neurosurg 110:462-8. 2009
    ..The goal of this study was to clarify the relationship between iron overload and AQP4 expression and to characterize the effects of the iron chelator deferoxamine (DFO) on delayed brain edema after experimental ICH...
  66. ncbi [Clinical features of NMO according to brain MRI findings]
    Yuko Shimizu
    Department of Neurology, Tokyo Women s Medical University School of Medicine, Tokyo, Japan
    Brain Nerve 62:933-43. 2010
    ..This review presents the characteristic clinical features of NMO according to the brain MRI findings and the features that can be used to distinguish NMO from MS...
  67. ncbi Aquaporins in brain: distribution, physiology, and pathophysiology
    Jerome Badaut
    Departement de Neurochirurgie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
    J Cereb Blood Flow Metab 22:367-78. 2002
    ..b>Aquaporin 4 may also play a role in pathophysiologic conditions, as shown by the reduced edema formation observed after ..
  68. pmc A role for aquaporin-4 during induction of form deprivation myopia in chick
    Melinda J Goodyear
    School of Psychological Science, La Trobe University, Melbourne, Australia
    Mol Vis 14:298-307. 2008
    ..Thus, on the basis of the hyperosmolarity seen across the retina and choroid of hatchling chickens made myopic by form deprivation (FD), we predicted an upregulation of retinal AQP4 expression during induction of myopia...
  69. ncbi Altered expression of aquaporin 4 and H(+)/K(+)-ATPase in the stomachs of peptide YY (PYY) transgenic mice
    M Carmosino
    Dipartimento di Fisiologia Generale ed Ambientale, Via Amendola 165 A, 70126, Bari, Italy
    Biol Cell 97:735-42. 2005
    ..In the present study, we investigated the expression of AQP4 (aquaporin 4) water channel and H(+)/K(+)-ATPase in stomachs from both control and transgenic mice.
  70. ncbi Aquaporin-4 expression in adult and developing mouse and rat kidney
    Y H Kim
    Department of Anatomy, Catholic University Medical College, Seoul, Korea
    J Am Soc Nephrol 12:1795-804. 2001
    ..These results suggest that there are differences in transepithelial water transport between mouse and rat or that additional, not yet identified water channels exist in the rat proximal tubule...
  71. ncbi [Mechanism of potassium channel in hypoxia-ischemic brain edema: experiment with neonatal rat astrocyte]
    Xue mei Fu
    Department of Pediatrics, Chengdu Materal and Children Health Hospital, Chengdu 610031, China
    Zhonghua Yi Xue Za Zhi 88:2862-6. 2008
    ..To investigate the mechanism of potassium channel in brain edema caused by hypoxia-ischemia (HI)...
  72. doi High salt loading alters the expression and localization of glial aquaporins in rat retina
    Yaowu Qin
    EENT Hospital, Eye Institute, Fudan University, Fenyang Road 83, Shanghai, China
    Exp Eye Res 89:88-94. 2009
    ..Our findings indicate that high salt loading may induce neural retina edema, and that altered glial cell-mediated water transport via AQP channels in the retina may be one of the reasons for intracellular edema in the neural retina...
  73. pmc Changes in ocular aquaporin expression following optic nerve crush
    Adnan Dibas
    Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA
    Mol Vis 16:330-40. 2010
    ..This study was designed to analyze changes in the expression of AQP4 (water selective channel) and AQP9 (water and lactate channel) following ONC in the rat...
  74. pmc Antibody to aquaporin 4 in the diagnosis of neuromyelitis optica
    Friedemann Paul
    Cecilie Vogt Clinic for Molecular Neurology, Charite, University Medicine Berlin, Berlin, Germany
    PLoS Med 4:e133. 2007
    ..The binding sites of this autoantibody were reported to colocalize with aquaporin 4 (AQP4) water channels. Thus we hypothesized that AQP4 antibodies in fact characterize NMO patients.
  75. ncbi Pathogenic potential of IgG binding to water channel extracellular domain in neuromyelitis optica
    S R Hinson
    Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Neurology 69:2221-31. 2007
    ..Evidence for antigen-specific autoantibody pathogenicity is lacking...
  76. doi Immunolocalization of water channel aquaporins in the vomeronasal organ of the rat: expression of AQP4 in neuronal sensory cells
    Abduxukur Ablimit
    Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Chem Senses 33:481-8. 2008
    ..The present findings suggest a unique water-handling feature in neuronal sensory cells in the vomeronasal organ...
  77. doi Antiacquaporin 4 antibodies detection by different techniques in neuromyelitis optica patients
    R Fazio
    Institute of Experimental Neurology INSPE, Department of Neurology, San Raffaele Scientific Institute, Milan, Italy
    Mult Scler 15:1153-63. 2009
    ..However, a consensus on seroprevalence and optimal detection method has not yet been reached...
  78. doi Neurological autoimmunity targeting aquaporin-4
    S R Hinson
    Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Neuroscience 168:1009-18. 2010
    ..Development of effective, and potentially curative, therapies requires validated models of the disease, in animals and cell culture systems...
  79. ncbi The relationship between aquaporin-4 expression and blood-brain and spinal cord barrier permeability following experimental autoimmune encephalomyelitis in the rat
    Xiang Nan Huang
    Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, People s Republic of China
    Anat Rec (Hoboken) 294:46-54. 2011
    b>Aquaporin 4(AQP4) is a water channel protein strongly expressed in the central nervous system in perimicrovessel astrocyte foot processes, the glia limitans, and ependyma...
  80. ncbi Modulation of AQP4 expression by the selective V1a receptor antagonist, SR49059, decreases trauma-induced brain edema
    Keisuke Taya
    Department of Neurosurgery, Virginia Commonwealth University Medical Center, 1101 East Marshall Street, P O Box 980508, Richmond, VA 23298 0508, USA
    Acta Neurochir Suppl 102:425-9. 2008
    ..We, therefore, examined whether the selective V1a receptor antagonist, SR49059, reduces brain edema by modulating AQP4 expression following cortical contusion injury (CCI)...
  81. doi Immunoglobulin M antibodies to aquaporin-4 in neuromyelitis optica and related disorders
    Sven Jarius
    Division of Molecular Neuroimmunology, Department of Neurology, University of Heidelberg, Heidelberg, Germany
    Clin Chem Lab Med 48:659-63. 2010
    ..Testing for AQP4-IgM may be of importance since 20%-30% of patients with NMO are negative for AQP4-IgG. Moreover, IgM antibodies are more potent activators of complement compared with IgG, and are detectable at NMO lesional sites...
  82. ncbi Loss of aquaporin 4 in lesions of neuromyelitis optica: distinction from multiple sclerosis
    T Misu
    Department of Neurology, Tohoku University School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
    Brain 130:1224-34. 2007
    ..antibody (NMO-IgG) was found in the sera from patients with NMO, and its target antigen was identified as aquaporin 4 (AQP4) water channel protein, mainly expressed in astroglial foot processes...
  83. pmc Anti-aquaporin 4 antibody in Japanese multiple sclerosis: the presence of optic spinal multiple sclerosis without long spinal cord lesions and anti-aquaporin 4 antibody
    Masami Tanaka
    MS Centre, Utano National Hospital, Kyoto, Japan
    J Neurol Neurosurg Psychiatry 78:990-2. 2007
    Anti-aquaporin 4 (AQP4) antibodies were found in patients with neuromyelitis optica (NMO) and Japanese optic-spinal multiple sclerosis (OSMS).
  84. doi Intractable hiccup and nausea in neuromyelitis optica with anti-aquaporin-4 antibody: a herald of acute exacerbations
    T Takahashi
    Department of Neurology, Tohoku University Graduate School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
    J Neurol Neurosurg Psychiatry 79:1075-8. 2008
    ..Recent studies have strongly suggested that the pathogenesis of NMO is closely associated with anti-aquaporin-4 (AQP4) antibody. However, clinical implications of IHN and the relationship with anti-AQP4 antibody remain unknown...
  85. doi Multimodality-evoked potential study of anti-aquaporin-4 antibody-positive and -negative multiple sclerosis patients
    Akihiro Watanabe
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Japan
    J Neurol Sci 281:34-40. 2009
    ..These findings suggest a distinctive nature of optic nerve lesions between anti-AQP4 antibody-positive and -negative patients; lesions are supposed to be more necrotic in the former group and more demyelinating in the latter...
  86. ncbi Characterization of aquaporin-4 in muscle and muscular dystrophy
    Rachelle H Crosbie
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    FASEB J 16:943-9. 2002
    ..Taken together, our data demonstrate that AQP4 loss in skeletal muscle correlates with muscular dystrophy and is a common feature of pathogenesis...
  87. ncbi [A case of anti-aquaporin 4 antibody-positive Sjögren syndrome associated with a relapsed myelitis in pregnancy]
    Jun Tsugawa
    Department of Neurology, Fukuoka University
    Rinsho Shinkeigaku 50:27-30. 2010
    ..Intravenous steroid administration seems safe and effective against relapse of NMO during pregnancy...
  88. ncbi Neuromyelitis optica and opticospinal multiple sclerosis: Mechanisms and pathogenesis
    Jun Ichi Kira
    Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Pathophysiology 18:69-79. 2011
    ..Possible mechanisms for NMO and OSMS are discussed in this review...
  89. ncbi [What I have learned and accomplished through research on multiple sclerosis (MS)]
    Yasuto Itoyama
    Department of Neurology, Tohoku University
    Rinsho Shinkeigaku 49:699-707. 2009
    ..The most recent and important discovery that NMO-IgG reacts specifically with aquaporin 4 (AQP4), which is a water channel localized in astrocytes, opened new avenues for understanding the pathogenesis ..
  90. ncbi Impaired hearing in mice lacking aquaporin-4 water channels
    J Li
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 276:31233-7. 2001
    ..AQP4 may facilitate rapid osmotic equilibration in epithelial cells in the organ of Corti, which are subject to large K(+) fluxes during mechano-electric signal transduction...
  91. pmc Delayed onset of brain edema and mislocalization of aquaporin-4 in dystrophin-null transgenic mice
    Zsolt Vajda
    Water and Salt Research Center, University of Aarhus, DK 8000 Aarhus, Denmark
    Proc Natl Acad Sci U S A 99:13131-6. 2002
    ..Moreover, these results predict that interference with the subcellular localization of AQP4 may have therapeutic potential for delaying the onset of impending brain edema...
  92. ncbi Cerebral cortical aquaporin-4 expression in brain edema following cardiac arrest in rats
    Feng Xiao
    Department of Emergency Medicine, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130, USA
    Acad Emerg Med 11:1001-7. 2004
    ..The objective of this study was to determine the expression of cerebral cortex aquaporin (AQP)-4, a member of a family of membrane water-channel proteins, in brain edema formation following normothermic or hypothermic CA...
  93. pmc Aquaporin-4-binding autoantibodies in patients with neuromyelitis optica impair glutamate transport by down-regulating EAAT2
    Shannon R Hinson
    Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Exp Med 205:2473-81. 2008
    ..Thus, binding of NMO-IgG to astrocytic AQP4 initiates several potentially neuropathogenic mechanisms: complement activation, AQP4 and EAAT2 down-regulation, and disruption of glutamate homeostasis...
  94. doi Anti-aquaporin-4 antibody-positive optic neuritis
    Mineo Takagi
    Niigata University, Japan
    Acta Ophthalmol 87:562-6. 2009
    ..We present three cases of optic neuritis (ON) where the patients tested positive for AQP4-Ab, but showed no neurological signs...
  95. ncbi [Effects of transient cerebral ischemia and reperfusion injury on expression of aquaporin-4 and apoptosis in hippocampal neurons of aged animals: experiment with rats]
    Ming Shan Wang
    Department of Anesthesiology, Affiliated Hospital of Qingdao University, Qingdao 266071, China
    Zhonghua Yi Xue Za Zhi 88:2504-7. 2008
    ..To investigate the effects of transient cerebral ischemia and reperfusion injury on brain edema and apoptosis hippocampal neurons of aged animals...
  96. pmc Anti-aquaporin-4 antibodies in Devic's neuromyelitis optica: therapeutic implications
    Romain Marignier
    Service de Neurologie A and EDMUS Coordinating Center, Hopital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, Lyon, F 69677, France
    Ther Adv Neurol Disord 3:311-21. 2010
    ..In this review, we summarize the present and future therapeutic implications generated by the discovery of the various pathogenic mechanisms of AQP4 Abs in DNMO pathophysiology...
  97. pmc Linking binge alcohol-induced neurodamage to brain edema and potential aquaporin-4 upregulation: evidence in rat organotypic brain slice cultures and in vivo
    Kumar Sripathirathan
    CoMentis Inc, Oklahoma City, Oklahoma, USA
    J Neurotrauma 26:261-73. 2009
    ....
  98. ncbi Role of aquaporin-4 water channel in the development and integrity of the blood-brain barrier
    B Nico
    Department of Human Anatomy and Histology, University of Bari Medical School, I 70124 Bari, Italy
    J Cell Sci 114:1297-307. 2001
    In this study, we have investigated the expression of aquaporin 4 during blood-brain barrier development in the optic tectum of chick embryos and newly hatched chicks, by means of western-blot, reverse transcriptase-polymerase chain ..
  99. ncbi Absence of aquaporin-4 expression in lesions of neuromyelitis optica but increased expression in multiple sclerosis lesions and normal-appearing white matter
    Colin Sinclair
    Neuropathology Laboratory, Institute of Pathology, Royal Group of Hospitals Trust, and School of Medicine and Dentistry, Belfast, N Ireland, UK
    Acta Neuropathol 113:187-94. 2007
    ..The finding of increased AQP4 expression in MS lesions in contrast to the lack of expression in NMO lesions may suggest different mechanisms of initiation and progression between the two disease states...
  100. ncbi Anti-aquaporin-4 antibody is involved in the pathogenesis of NMO: a study on antibody titre
    Toshiyuki Takahashi
    Department of Neurology, Tohoku University Graduate School of Medicine, 1 1 Seiryomachi, Aobaku, Sendai 980 8574, Japan
    Brain 130:1235-43. 2007
    ....
  101. doi Aquaporin-4 orthogonal arrays of particles are the target for neuromyelitis optica autoantibodies
    Grazia Paola Nicchia
    Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics CEGBA, University of Bari, Bari, Italy
    Glia 57:1363-73. 2009
    ..Finally, water transport measurements show that NMO-IgG treatment does not significantly affect AQP4 function. In conclusion, our results suggest for the first time that OAP assemblies are required for NMO-IgG to recognize AQP4...

Research Grants76

  1. Alan S Verkman; Fiscal Year: 2016
    ..We have developed both monoclonal antibody and small-molecule approaches, which will be optimized and used in mouse models to obtain proof-of-concept that blocking NMO-IgG binding to AQP4 can reduce ocular pathology in NMO. ..
  2. HANS CHRISTIAN VON BUEDINGEN; Fiscal Year: 2015
    ..These studies will elucidate pathologically relevant antibody-antigen interactions and are expected to serve as an important foundation for the development of disease-specific therapies and novel biomarkers. ..
  3. Jeffrey L Bennett; Fiscal Year: 2016
    ....
  4. Michael Levy; Fiscal Year: 2016
    ..Levy will have 75% protected time to spend in the lab and use the rest of the time to translate his basic science work to the bedside in the NMO clinic and on the inpatient neurology service at Johns Hopkins Hospital. ..
  5. SEAN JOSEPH PITTOCK; Fiscal Year: 2014
    ....
  6. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    CHRISTINA ROSE MARMAROU; Fiscal Year: 2012
    ..This proposal will address the fundamental mechanism of brain swelling and how it can be blocked pharmacologically. ..
  7. BIOMECHANICS OF BRAIN EDEMA AND INTRACRANIAL PRESSURE
    Anthony Marmarou; Fiscal Year: 2007
    ..Taken in concert, these studies will provide a more indepth understanding of the role of aquaporins in brain injury and introduce novel therapeutic approaches for treating brain edema. ..
  8. NFkB in the Astrocyte Swelling/Brain Edema with Acute Liver Failure
    MICHAEL DAVID NORENBERG; Fiscal Year: 2012
    ..We propose to examine the role of the transcription factor NF-:B as a critical intermediary responsible for causing brain swelling in ALF. ..
  9. MICHAEL DAVID NORENBERG; Fiscal Year: 2016
    ....
  10. AQP4 and JNK Inhibition Together Reduce Edema and Excitotoxic Injury in jTBI
    Jerome Badaut; Fiscal Year: 2013
    ....
  11. Role of Aquaporin-0 for cell-to-cell adhesion and lens transparency
    Kulandaiappan Varadaraj; Fiscal Year: 2013
    ..The objectives will be pursued using structure-function approach and performing cytological, biochemical and molecular biological experiments as appropriate to verify the results. ..
  12. Alan S Verkman; Fiscal Year: 2014
    ....
  13. Maiken Nedergaard; Fiscal Year: 2016
    ..The proposed studies will provide fundamental new insight into cognitive impairment in vascular dementia, and will likely also improve our understanding of the pathophysiology of brain injury following stroke and head trauma. ..
  14. Structure Analysis of Aquaporin Membrane Channels
    Mark Yeager; Fiscal Year: 2006
    ..In addition, this project will provide insight into the molecular basis for channel regulation and will contribute to the general principles that govern conformational changes of membrane proteins. ..
  15. Aquaporins and Neuropathic Pain after Spinal Cord Injury
    Olivera Nesic Taylor; Fiscal Year: 2009
    ..Our hypothesis on the involvement of AQP4, AQP1 and impaired osmoregulation in the pathogenesis of neuropathic pain conditions after SCI is novel and has not been previously studied. Project Description Page 6 ..
  16. Maiken Nedergaard; Fiscal Year: 2016
    ..abstract_text> ..
  17. ROLE OF AQUAPORIN (AQP) 5 IN SALIVARY SECRETION
    Anil Menon; Fiscal Year: 2004
    ....
  18. Molecular and Cellular Analyses of Aquaporins in Lung
    LANDON KING; Fiscal Year: 2005
    ..These studies should provide insight into mechanisms regulating expression of AQP1 and AQP5, and establish a framework from which to better understand their role in the pathophysiology of the respiratory tract. ..
  19. STRUCTURE/FUNCTION OF MEMBRANE PROTEIN CHANNELS
    ALOK MITRA; Fiscal Year: 2002
    ..The pore-forming anthrax toxin has been shown to have a potential for delivery of macromolecules across the bilayer. Thus structural studies on these systems will potentially have impact on structure-based drug design. ..
  20. Debasish Sinha; Fiscal Year: 2015
    ....
  21. Lorenz P Studer; Fiscal Year: 2014
    ..establish a glial specific reporter human ESC lines targeting the glial fibrillary aciic protein (GFAP) and the aquaporin 4 (AQP4) to identify astrocytes and glial competent NSCs...
  22. Refinement of lead compounds to modulate water permeability through AQP4
    Marc F Pelletier; Fiscal Year: 2012
    ..In this Phase II SBIR, Aeromics plans to use this information to create new drugs that it will test in animal models of cerebral edema and stroke. The next step will be to refine the drugs further and prepare them for human testing. ..
  23. Tonia S Rex; Fiscal Year: 2016
    ..There is also evidence that EPO can decrease the levels of Aquaporin 4 (AQP4), a water channel that induces astrocyte hypertrophy in the brain...
  24. Scale-Up from Pilot To High-Throughput Screen for Inhibitors of Aquaporin-4
    Marc F Pelletier; Fiscal Year: 2011
    ..These compounds are currently under development in a Phase II SBIR. With the current screening technologies in place, we are ready to scale-up our drug discovery program to complete a more thorough search for new drug candidates. ..
  25. Development of a high-throughput screen for inhibitors of water permeability thro
    Marc Pelletier; Fiscal Year: 2009
    ..The goal of this project is to develop the technology to identify new drugs for treating cerebral edema while maintaining the delivery of oxygen to the brain. ..
  26. Mechanisms of rapid paravascular fluid transport in the brain
    Jeffrey J Iliff; Fiscal Year: 2012
    ..Here, the aim is to define how water flows along the outside of blood vessels in the brain, and to determine if the stoppage of this flow contributes to cerebral edema following stroke. ..
  27. Multiparametric Classification of Muscle Damage in Inflammatory Myopathy
    Jane Park; Fiscal Year: 2013
    ..In this study, we would like to develop new tools, based on non-invasive imaging methods, that will allow clinicians to describe muscle damage brought about inflammatory muscle disease in a more specific and quantitative fashion. ..
  28. TRP and AQP Channels: Modulation of Function, Raft Location by Membrane Lipids
    THOMAS J MC INTOSH; Fiscal Year: 2012
    ..abstract_text> ..
  29. Regulation of Syntrophin Function
    Stanley C Froehner; Fiscal Year: 2013
    ..Deciphering the role of phosphorylation and phosphatidylinositol binding in this process is essential to understanding the signaling function of the DPC and its role in muscle diseases. ..
  30. HMGB1 and Traumatic Brain Injury
    Krishnan M Dhandapani; Fiscal Year: 2013
    ..The results of these studies may support the future development of novel therapeutics directed against this pathway to limit neurological injury following head trauma. ..
  31. Alan S Verkman; Fiscal Year: 2016
    ..In addition, the proposed studies will establish novel and widely applicable methodology to measure protein dynamics and interactions in cell plasma membrane and in the extracellular space. ..
  32. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  33. KININ REGULATION OF COLLECTING DUCT H+ EFFLUX
    YURII MUKHIN; Fiscal Year: 2002
    ..My goal in performing these studies will be to obtain training in signal transduction methods so that I can eventually integrate my background in physics into the study of renal problems. ..
  34. PHYSIOLOGY OF POTASSIUM CHANNELS IN RETINAL GLIAL CELLS
    Paulo Kofuji; Fiscal Year: 2009
    ..The molecular understanding of the events that are central to their regulated expression is therefore essential for developing our knowledge in the area of retinal function in health and in diseases. ..
  35. FUNCTION & REGULATION OF GASTRIC NA/K/2CL COTRANSPORTERS
    Christian Lytle; Fiscal Year: 2002
    ..Completion of these aims will begin to clarify the functional consequences of the migration-associated differentiation of parietal cells. ..
  36. Role of Aquaporin-4 Water Channels in Cerebral Edema
    Geoffrey Manley; Fiscal Year: 2009
    ..If AQP4 is proven to be important, as anticipated, then modulation of AQP4 function could provide a novel therapeutic strategy for the treatment of cerebral edema. ..
  37. Mechanisms of Sec61-Mediated Polytopic Protein Integration into the ER
    Brian Conti; Fiscal Year: 2009
    ..Using a model polytopic protein, aquaporin 4 (AQP4), the current proposal determines the manner in which transmembrane segments move from the endoplasmic ..
  38. MECHANISMS OF WATER TRANSPORT IN RENAL EPITHELIA
    Alan Verkman; Fiscal Year: 2008
    ..Methods will include fluorescence photobleaching recovery, energy transfer and ratio imaging. Also, specific water/solute transporting properties (Pd, c^)of the mammalian aquaporins will be measured. ..
  39. Role of airway submucosal glands in CF lung disease
    Alan S Verkman; Fiscal Year: 2010
    ..The data will provide a rigorous scientific rationale for use of small-molecules in CF therapy, and establish widely useful assays to prioritize small molecules for clinical development. ..
  40. PHYSIOLOGY AND MOLECULAR BIOLOGY OF LUNG WATER TRANSPORT
    Alan Verkman; Fiscal Year: 2007
    ..abstract_text> ..
  41. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2006
    ..abstract_text> ..
  42. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2001
    ..These studies will address the hypothesis that the membrane skeleton of these cells is a mobile, dynamic structure. ..
  43. Varenicline and Smoking Cessation in Schizophrenia
    SEYYED HOSSEIN FATEMI; Fiscal Year: 2010
    ..Such outcomes will be significant, because they will offer a new treatment for smoking cessation in this vulnerable population. ..
  44. Prenatal Virally Induced Brain Disorder in Mouse
    SEYYED FATEMI; Fiscal Year: 2009
    ..In addition, it is expected that the results will provide clues that will lead to fundamental advances in our knowledge of the pathogenesis of schizophrenia and, therefore, of how it can be prevented and treated. ..
  45. Zeiss LSM 510 Laser Scanning Microscope
    JOHN RASH; Fiscal Year: 2002
    ..The DIC Board of Directors will meet bi-monthly to insure continued efficient scheduling and operation of the replacement confocal microscope, to consider future upgrades, and to establish or change DIC access policies. ..
  46. ALTERED KCa CHANNEL EXPRESSION IN DEVELOPING CEREBELLUM
    ANDREA YOOL; Fiscal Year: 2002
    ..The rat provides a useful model for studying postnatal cerebellar development and neuropathological damage, and may provide insights into methods to manipulate developmental abnormalities in the cerebellum. ..
  47. Connexins in Neuronal and Glial Gap Junctions in the Central Nervous System
    JOHN RASH; Fiscal Year: 2007
    ..abstract_text> ..
  48. HIV-1 protein neurotoxicity and ethanol pre-conditioning
    Michael Collins; Fiscal Year: 2006
    ..abstract_text> ..
  49. Gap Junctions and Connexins in Developing CNS
    JOHN RASH; Fiscal Year: 2005
    ....
  50. Interferon Gamma Polymorphisms and Gender Bias in MS
    Brian Weinshenker; Fiscal Year: 2004
    ..If successful, this paradigm could be applied to a number of similar genetic association studies. ..
  51. Temporal lobe epilepsy - validation of a new animal model
    Tore Eid; Fiscal Year: 2008
    ..More effective therapies of epilepsy are therfore needed. This proposal seeks to validate a new animal model that may greatly speed up the search for better therapies against this disorder. ..
  52. Aquaporin 5 (AQP5) in Lung Fluid Homeostasis
    CARISSA KRANE; Fiscal Year: 2004
    ..This combinatorial approach will provide valuable information for the identification of new mechanistic pathways critical for the development of novel drug targets for the treatment of asthma. ..
  53. Genetic Signatures in Human Temporal Lobe Epilespy
    Nihal de Lanerolle; Fiscal Year: 2005
    ..abstract_text> ..
  54. PEPTIDE REGULATION OF THE CHOROID PLEXUS-CSF SYSTEM
    Conrad Johanson; Fiscal Year: 2002
    ..Our long-term goal is to delineate the multifunctional roles of CP in brain fluid homeostasis. ..
  55. Genetics of Severe Systemic Lupus Erythematosus (SLE) defined by thrombocytopenia
    R Scofield; Fiscal Year: 2007
    ..Finally, Specific Aim 4 will assemble a new cohort of families multiplex for SLE so that linkages and associations as well as particular polvmorphisms can be prospectively confirmed. ..
  56. Blood-brain barrier gene delivery in knock-out mice.
    EAIN CORNFORD; Fiscal Year: 2005
    ..abstract_text> ..
  57. Abnormalities in Keratoconus Corneas
    MARIA KENNEY; Fiscal Year: 2003
    ..These studies will provide fundamental insights into KC pathogensis and may provide a basis for therapy. ..
  58. Herpes Simplex Virus, Early Brain Injury and Epilepsy
    Tore Eid; Fiscal Year: 2004
    ..If a viral causation of TLE is established, then this would not only open new avenues for prevention and control of this disorder, but also improve our understanding of viral-induced brain injury. ..
  59. Role of reactive oxygen species in synaptic transmission
    Michael Levy; Fiscal Year: 2002
    ..In addition to characterizing the VDAC knockout mouse, I will determine the downstream effectors involved in ROS signaling during synaptic transmission. ..
  60. RECEPTORS FROM MOSQUITO AEDES AEGYPTI EXCRETORY SYSTEM
    PATRICIA PIETRANTONIO; Fiscal Year: 2002
    ..Aegypti. To test the hypothesis, the applicant proposes to clone the receptor gene, localize the tissue expression by in situ hybridization and immunohistochemistry and express the protein in transformed cells. ..
  61. EXTRACELLULAR MATRIX ABNORMALITIES IN CORNEAL EDEMA
    MARIA KENNEY; Fiscal Year: 2002
    ....
  62. Spectrum of Autoimmune Gastrointestinal Dysmotility
    Vanda Lennon; Fiscal Year: 2009
    ..l.dysmotilities, and justify early consideration of immunomodulatory therapies. The proposed research also has high clinical relevance to cancer immunity. ..
  63. High Field MRI Scanner for Animal Studies
    Ponnada Narayana; Fiscal Year: 2003
    ..Organizational and financial plans for administering and maintaining the scanner are described. To the best of the PI's knowledge, this will be the only facility of its kind in Houston and the Southwest part of the USA. ..
  64. MRI of Angiogenesis in Spinal Cord Injury
    Ponnada Narayana; Fiscal Year: 2009
    ..In addition, it is possible to modulate angiogenesis in conjunction with other treatments, such as cellular grafts to further enhance the recovery from SCI. Thus the proposed studies have a very high degree of clinical relevance. ..
  65. 3 T Whole Body Magnetic Resonance Scanner
    Ponnada Narayana; Fiscal Year: 2004
    ..Strong institutional commitment and financial plans for operating the scanner, particularly the long-term maintenance, are described. ..
  66. TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUM
    Jorge Fischbarg; Fiscal Year: 2004
    ..The understanding to be gained may provide insights on how to modulate fluid transport so as to offset endothelial dysfunction leading to the loss of normal vision. ..
  67. Air Pollution and Central Nervous System Disease
    Lilian Calderon Garciduenas; Fiscal Year: 2008
    ..The Award will give me the opportunity to build new skills in 4 areas: Molecular Biology, DNA Damage, Developmental / Neurodegenerative Neuropathology, and to gain experience in grantsmanship. [unreadable] [unreadable]..
  68. Sjogren's model with anti-Ro/La and Salivary Pathology and Dysfunction
    R Hal Scofield; Fiscal Year: 2010
    ..Improved understanding of the disease at a fundamental level is possible through study of animal models. Such understanding may lead to improved recognition and treatment. ..
  69. MOLECULAR PHYSIOLOGY AND STRUCTURE OF AQUAPORINS
    Alfred Van Hoek; Fiscal Year: 2002
    ....
  70. MR Image Analysis in MS: Identification of a Surrogate
    Ponnada Narayana; Fiscal Year: 2002
    ..While the main emphasis is on MS, this system should be readily adaptable to investigate and manage various neurological disorders that require accurate determination of tissue volumes and their temporal change. ..
  71. PET AND THE BLOOD BRAIN BARRIER IN HUMAN EPILEPSY
    EAIN CORNFORD; Fiscal Year: 2002
    ..abstract_text> ..
  72. Vestibular Pathology in Meniere's Disease
    Akira Ishiyama; Fiscal Year: 2007
    ..or with alterations in neuronal number or size? 6) What are the morphometric correlates to abnormalities on traditional vestibular testing: caloric paresis, and decreased gain and time constant on step rotational testing? ..
  73. The X chromosome in systemic lupus erythematosus
    R Hal Scofield; Fiscal Year: 2010
    ..This proposal will determine whether the presence of two X chromosomes is a risk factor for women and men with Klinefelter's syndrome (47,XXY). ..