AP2M1

Summary

Gene Symbol: AP2M1
Description: adaptor related protein complex 2 mu 1 subunit
Alias: AP50, CLAPM1, mu2, AP-2 complex subunit mu, AP-2 mu 2 chain, HA2 50 kDA subunit, adaptin-mu2, adaptor protein complex AP-2 subunit mu, adaptor-related protein complex 2 subunit mu, clathrin adaptor complex AP2, mu subunit, clathrin assembly protein complex 2 medium chain, clathrin assembly protein complex 2 mu medium chain, clathrin coat adaptor protein AP50, clathrin coat assembly protein AP50, clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein
Species: human

Top Publications

  1. ncbi Characterization of a protein phosphatase 2A holoenzyme that dephosphorylates the clathrin adaptors AP-1 and AP-2
    Doris Ricotta
    Department of Biomedical Science and Biotechnology, University of Brescia, 25100 Brescia, Italy
    J Biol Chem 283:5510-7. 2008
  2. pmc Role of the HIV-1 Matrix Protein in Gag Intracellular Trafficking and Targeting to the Plasma Membrane for Virus Assembly
    Ruba H Ghanam
    Department of Microbiology, University of Alabama at Birmingham Birmingham, AL, USA
    Front Microbiol 3:55. 2012
  3. pmc A diacidic motif in human immunodeficiency virus type 1 Nef is a novel determinant of binding to AP-2
    O Wolf Lindwasser
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 82:1166-74. 2008
  4. ncbi Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif
    H M Craig
    Department of Pathology and, Department of Medicine, San Diego Veterans Affairs Medical Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, California, 92093 0679, USA
    Virology 271:9-17. 2000
  5. ncbi CD4 down-regulation by HIV-1 and simian immunodeficiency virus (SIV) Nef proteins involves both internalization and intracellular retention mechanisms
    Jeremy J Rose
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:7413-26. 2005
  6. doi Viral genetic determinants of nonprogressive HIV type 1 subtype C infection in antiretroviral drug-naive children
    D B Tzitzivacos
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, Parktown, Johannesburg 2193, South Africa
    AIDS Res Hum Retroviruses 25:1141-8. 2009
  7. ncbi HIV-1 matrix protein: a mysterious regulator of the viral life cycle
    Alissa Bukrinskaya
    D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow 123098, RF, Russia
    Virus Res 124:1-11. 2007
  8. pmc The 50 kDa protein subunit of assembly polypeptide (AP) AP-2 adaptor from clathrin-coated vesicles is phosphorylated on threonine-156 by AP-1 and a soluble AP50 kinase which co-purifies with the assembly polypeptides
    A Pauloin
    INRA, Laboratoire de Biologie Cellulaire et Moleculaire, Centre de Recherche de Jouy, Jouy en Josas, France
    Biochem J 296:409-15. 1993
  9. pmc HIV-1 Tat enters T cells using coated pits before translocating from acidified endosomes and eliciting biological responses
    Agnès Vendeville
    UMR 5539 CNRS, Département Biologie Santé, Case 107, Universite Montpellier II, 34095 Montpellier Cedex 5, France
    Mol Biol Cell 15:2347-60. 2004
  10. ncbi Serine phosphorylation-independent downregulation of cell-surface CD4 by nef
    J V Garcia
    Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Nature 350:508-11. 1991

Detail Information

Publications222 found, 100 shown here

  1. ncbi Characterization of a protein phosphatase 2A holoenzyme that dephosphorylates the clathrin adaptors AP-1 and AP-2
    Doris Ricotta
    Department of Biomedical Science and Biotechnology, University of Brescia, 25100 Brescia, Italy
    J Biol Chem 283:5510-7. 2008
    ..large subunits (alpha- and beta2-adaptins) as well as at a single threonine residue (Thr-156) of the medium subunit mu2. Phosphorylation of mu2 is necessary for efficient cargo recruitment, whereas the functional context of the large ..
  2. pmc Role of the HIV-1 Matrix Protein in Gag Intracellular Trafficking and Targeting to the Plasma Membrane for Virus Assembly
    Ruba H Ghanam
    Department of Microbiology, University of Alabama at Birmingham Birmingham, AL, USA
    Front Microbiol 3:55. 2012
    ..In this review, we summarize the current understanding of the structural and functional role of MA in HIV replication...
  3. pmc A diacidic motif in human immunodeficiency virus type 1 Nef is a novel determinant of binding to AP-2
    O Wolf Lindwasser
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 82:1166-74. 2008
    ....
  4. ncbi Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif
    H M Craig
    Department of Pathology and, Department of Medicine, San Diego Veterans Affairs Medical Center, University of California at San Diego, 9500 Gilman Drive, La Jolla, California, 92093 0679, USA
    Virology 271:9-17. 2000
    ..The interactions with mu1 and mu3 were markedly stronger than the interaction with mu2. Leucine residues of the sorting motif were required for the interactions with mu3 and mu2 and contributed to the ..
  5. ncbi CD4 down-regulation by HIV-1 and simian immunodeficiency virus (SIV) Nef proteins involves both internalization and intracellular retention mechanisms
    Jeremy J Rose
    Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:7413-26. 2005
    ..In contrast, AP-2-mediated enhanced endocytosis constitutes the predominant mechanism for SIV (MAC-239 strain) Nef-induced down-regulation of human CD4 in human cells...
  6. doi Viral genetic determinants of nonprogressive HIV type 1 subtype C infection in antiretroviral drug-naive children
    D B Tzitzivacos
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, Parktown, Johannesburg 2193, South Africa
    AIDS Res Hum Retroviruses 25:1141-8. 2009
    ..For example, LT46 Nef is unable to bind AP-1 and AP-2 and therefore is inactive on CD4 endocytosis. The biological relevance of these findings requires further investigation...
  7. ncbi HIV-1 matrix protein: a mysterious regulator of the viral life cycle
    Alissa Bukrinskaya
    D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow 123098, RF, Russia
    Virus Res 124:1-11. 2007
    ..It is suggested that two MA fractions possess diverse functions and are involved in different stages of virus morphogenesis as key regulators of the viral life cycle...
  8. pmc The 50 kDa protein subunit of assembly polypeptide (AP) AP-2 adaptor from clathrin-coated vesicles is phosphorylated on threonine-156 by AP-1 and a soluble AP50 kinase which co-purifies with the assembly polypeptides
    A Pauloin
    INRA, Laboratoire de Biologie Cellulaire et Moleculaire, Centre de Recherche de Jouy, Jouy en Josas, France
    Biochem J 296:409-15. 1993
    b>AP50 is a subunit of the assembly polypeptide (AP) subclass AP-2 from bovine brain coated vesicles. It can be phosphorylated in vivo and in vitro on a threonine residue by means of the AP50 kinase activity associated with AP...
  9. pmc HIV-1 Tat enters T cells using coated pits before translocating from acidified endosomes and eliciting biological responses
    Agnès Vendeville
    UMR 5539 CNRS, Département Biologie Santé, Case 107, Universite Montpellier II, 34095 Montpellier Cedex 5, France
    Mol Biol Cell 15:2347-60. 2004
    ..Hence, Tat enters T cells essentially like diphtheria toxin, using clathrin-mediated endocytosis before low-pH-induced and Hsp90-assisted endosomal translocation. Cell responses are then induced from the cytosol...
  10. ncbi Serine phosphorylation-independent downregulation of cell-surface CD4 by nef
    J V Garcia
    Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Nature 350:508-11. 1991
    ..Also, nef can downregulate a CD4 triple mutant (Ser----Ala) that is neither phosphorylated nor down-regulated by phorbol esters, indicating that nef is acting by a different mechanism...
  11. ncbi Interaction of tyrosine-based sorting signals with clathrin-associated proteins
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 269:1872-5. 1995
    ..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
  12. pmc Targeting signals and subunit interactions in coated vesicle adaptor complexes
    L J Page
    Department of Clinical Biochemistry, University of Cambridge, Addenbrooke s Hospital, England
    J Cell Biol 131:619-30. 1995
    ..The subunit composition of the plasma membrane adaptor complex is alpha-adaptin, beta-adaptin, AP50, and AP17; while that of the TGN adaptor complex is gamma-adaptin, beta'-adaptin, AP47, and AP19...
  13. ncbi Interaction of CTLA-4 with the clathrin-associated protein AP50 results in ligand-independent endocytosis that limits cell surface expression
    E Chuang
    Gwen Knapp Center for Lupus and Immunology Research, Department of Molecular Genetics and Cell Biology, The University of Chicago, IL 60637, USA
    J Immunol 159:144-51. 1997
    ..The cytoplasmic domain of CTLA-4 was found to specifically bind to AP50, the medium chain subunit of AP-2 in both yeast two-hybrid and coimmunoprecipitation assays...
  14. pmc Interaction of CTLA-4 with AP50, a clathrin-coated pit adaptor protein
    Y Zhang
    Department of Molecular and Cellular Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 94:9273-8. 1997
    ..In this study, we demonstrate that CTLA-4 is associated with AP50, the medium chain of the clathrin-associated coated pit adaptor protein complex AP2...
  15. ncbi Interaction of endocytic signals from the HIV-1 envelope glycoprotein complex with members of the adaptor medium chain family
    H Ohno
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Virology 238:305-15. 1997
    ..These observations suggest that HIV-1 Env utilizes the protein sorting machinery of the host cells for internalization and sorting at various steps of the endocytic and biosynthetic pathways...
  16. ncbi A structural explanation for the recognition of tyrosine-based endocytotic signals
    D J Owen
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 282:1327-32. 1998
    ..for endocytosis by a cytoplasmic sequence motif, tyrosine-X-X-(hydrophobic residue), that is recognized by the mu2 subunit of AP2 adaptors...
  17. pmc Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins
    C Berlioz-Torrent
    CJF 97 03 INSERM, Interactions Moléculaires, Hôte Pathogène, Institut Cochin de Genetique Moleculaire, 75014 Paris, France
    J Virol 73:1350-61. 1999
    ....
  18. pmc The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter]
    S Wyss
    Institute of Microbiology, University of Lausanne, CH 1011 Lausanne, Switzerland
    J Virol 75:2982-92. 2001
    ..Finally, the C-terminal dileucine was shown to assist the membrane-proximal Y712 motif in restricting the cell surface expression of Env...
  19. ncbi Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2
    S M Morris
    Fred Hutchinson Cancer Research Center, Division of Basic Sciences, 1100 Fairview Avenue North, Seattle, WA 98109 1024, USA
    Traffic 2:111-23. 2001
    ..These findings suggest that in addition to previously reported signal-transduction functions, Dab2 could also act as an adaptor protein that may regulate protein trafficking...
  20. ncbi CK2 and GAK/auxilin2 are major protein kinases in clathrin-coated vesicles
    Viktor I Korolchuk
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Traffic 3:428-39. 2002
    ..The medium subunit of the AP2 adaptor complex (mu2) is not a substrate for CK2, but is phosphorylated by a second kinase that we show to be cyclin G-associated kinase ..
  21. ncbi The mu2 subunit of the clathrin adaptor AP-2 binds to FDNPVY and YppØ sorting signals at distinct sites
    Werner Boll
    Department of Cell Biology and The Center for Blood Research, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA
    Traffic 3:590-600. 2002
    ..with a photoactivated peptide probe to demonstrate the interaction between FDNPVY-containing peptides and the mu2 chain of purified AP-2 clathrin adaptors (the complexes responsible for plasma membrane sorting)...
  22. pmc Clathrin-mediated endocytosis in AP-2-depleted cells
    Alison Motley
    University of Cambridge, Department of Clinical Biochemistry, Cambridge Institute for Medical Research, Cambridge CB2 2XY, UK
    J Cell Biol 162:909-18. 2003
    We have used RNA interference to knock down the AP-2 mu2 subunit and clathrin heavy chain to undetectable levels in HeLaM cells...
  23. ncbi Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2
    Melissa Batonick
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Virology 342:190-200. 2005
    ..Together these data attribute a role to the AP-2 complex in the regulation of HIV-1 assembly/release...
  24. pmc Recruitment of the adaptor protein 2 complex by the human immunodeficiency virus type 2 envelope protein is necessary for high levels of virus release
    Beth Noble
    Department of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, Los Angeles, USA
    J Virol 80:2924-32. 2006
    ....
  25. pmc A conserved dileucine motif mediates clathrin and AP-2-dependent endocytosis of the HIV-1 envelope protein
    Rahel Byland
    Cell Biology Unit, MRC Laboratory for Molecular Cell Biology and Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT, United Kingdom
    Mol Biol Cell 18:414-25. 2007
    ....
  26. pmc Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor
    Rittik Chaudhuri
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bldg 18T, Rm 101, National Institutes of Health, Bethesda, MD 20892, USA
    J Virol 81:3877-90. 2007
    ..Together, these results support a model in which HIV-1 Nef downregulates CD4 by promoting its accelerated endocytosis by a clathrin/AP2 pathway...
  27. pmc Site-specific ubiquitination exposes a linear motif to promote interferon-alpha receptor endocytosis
    K G Suresh Kumar
    Department of Animal Biology and 2Mari Lowe Center for Comparative Oncology Research, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 179:935-50. 2007
    ....
  28. pmc Roles of AP-2 in clathrin-mediated endocytosis
    Emmanuel Boucrot
    Department of Cell Biology, Harvard Medical School and Immune Disease Institute at Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 5:e10597. 2010
    ....
  29. pmc HIV-1 envelope glycoprotein biosynthesis, trafficking, and incorporation
    Mary Ann Checkley
    Virus Cell Interaction Section, HIV Drug Resistance Program, NCI Frederick, Frederick, MD 21702 1201, USA
    J Mol Biol 410:582-608. 2011
    ..Here, we review our current understanding of HIV-1 Env glycoprotein trafficking and incorporation into virions...
  30. pmc Competition model for upregulation of the major histocompatibility complex class II-associated invariant chain by human immunodeficiency virus type 1 Nef
    Richard S Mitchell
    Department of Medicine, 1 Division of Biology, University of California, San Diego, La Jolla, CA 92093 0679, USA
    J Virol 82:7758-67. 2008
    ..These data indicate that Nef usurps AP-2 complexes to dysregulate Ii trafficking and potentially interfere with antigen presentation in the context of MHC-II...
  31. pmc Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking
    Richard S Mitchell
    Department of Medicine, University of California San Diego, La Jolla, CA, USA
    PLoS Pathog 5:e1000450. 2009
    ..Together, the data support a model in which Vpu co-opts the beta-TrCP/SCF E3 ubiquitin ligase complex to induce endosomal trafficking events that remove BST-2 from its site of action as a virion-tethering factor...
  32. ncbi Microarray analysis in the HSV-1 latently infected mouse trigeminal ganglion
    Shiro Higaki
    Department of Ophthalmology, Kinki University School of Medicine, Osaka, Japan
    Cornea 23:S42-7. 2004
    ..To review our previous studies regarding alterations in gene expression in HSV-1 latently infected mouse trigeminal ganglia (TGs) following treatment with immunosuppressants and hyperthermia...
  33. ncbi Molecular characterization of the 50- and 57-kDa subunits of the bovine vacuolar proton pump
    Z Zhou
    Division of Molecular Transport, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
    J Biol Chem 273:5878-84. 1998
    ..Biol. Chem. 269, 28509-25815). It has also been reported that the clathrin-coated vesicle proton pump contains AP50, a 50-kDa component of the AP-2 complex responsible for the assembly of clathrin-coated pits, and that AP50 is ..
  34. pmc Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules
    John S L Parker
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 76:4483-96. 2002
    ..When the mu2 proteins derived from T1L and the other laboratory's T3D isolate were expressed after transfection of their cloned ..
  35. ncbi Modulation of emesis by fentanyl and opioid receptor antagonists in Suncus murinus (house musk shrew)
    J A Rudd
    Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N T
    Eur J Pharmacol 374:77-84. 1999
    ..p.) or naloxonazine (35 mg/kg, i.p.). This indicates an involvement of mu2-opioid receptors within the brain to mediate the anti-emetic effect of fentanyl...
  36. ncbi Characterization of M-class genome segments of muscovy duck reovirus S14
    Yun Zhang
    Avian Infectious Disease Division of National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, PR China
    Virus Res 125:42-53. 2007
    ..Vero cells, which provides evidence that muA might be functional related to the mammalian reovirus (MRV) mu2. The M2 gene was 2155 nucleotides in length and was predicted to encode muB major outer capsid protein of 676 amino ..
  37. pmc A distributed set of interactions controls mu2 functionality in the role of AP-2 as a sorting adaptor in synaptic vesicle endocytosis
    Sung Hyun Kim
    Department of Biochemistry, Weill Cornell Medical College, New York, New York 10065, USA
    J Biol Chem 284:32803-12. 2009
    ..Using quantitative endocytosis assays at nerve terminals, we examined how endocytosis depends on the integrity of mu2 function...
  38. ncbi Stabilization of clathrin coats by the core of the clathrin-associated protein complex AP-2
    W Matsui
    Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 29:10791-8. 1990
    ..an AP-2 complex is an asymmetric structure consisting of one large alpha chain, one large beta chain, one medium AP50 chain, and one small AP17 chain. The complex has been shown to contain a core and two appendages...
  39. ncbi Interactions of annexins with the mu subunits of the clathrin assembly proteins
    Carl E Creutz
    Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908, USA
    Biochemistry 44:13795-806. 2005
    ..In a yeast two-hybrid screen for proteins that interact with the N-terminal domain of annexin A2 we identified the mu2 subunit of the clathrin assembly protein complex AP-2...
  40. doi Anticomplementary principles of a Chinese multiherb remedy for the treatment and prevention of SARS
    Ting Zhang
    Department of Pharmacognosy, School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
    J Ethnopharmacol 117:351-61. 2008
    ....
  41. ncbi Antenatal glucocorticoids attenuate activation of the inflammatory reaction and clotting in preterm lambs
    Anneke S Jaarsma
    Department of Pediatrics, University Hospital Groningen, Groningen, The Netherlands
    Biol Neonate 85:82-9. 2004
    ..Blood was used to determine oxygenation index, alveolar - arterial partial O(2) difference (AaDO(2)), AP50 titer (see text), polymorphonuclear leukocytes (PMNs), beta-glucuronidase, thrombin inhibition, activated partial ..
  42. ncbi Involvement of mu-opioid receptors in antinociception and inhibition of gastrointestinal transit induced by 7-hydroxymitragynine, isolated from Thai herbal medicine Mitragyna speciosa
    Kenjiro Matsumoto
    Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba 283 8555, Japan
    Eur J Pharmacol 549:63-70. 2006
    ..The antinociceptive effect of 7-hydroxymitragynine was significantly blocked by the mu1/mu2-opioid receptor antagonist beta-funaltrexamine hydrochloride (beta-FNA) and the mu1-opioid receptor-selective ..
  43. pmc Hydroxide-promoted core conversions of molybdenum-iron-sulfur edge-bridged double cubanes: oxygen-ligated topological PN clusters
    Mark L Hlavinka
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Inorg Chem 46:9192-200. 2007
    ..HQ- affords the clusters [(Tp)2Mo2Fe6S8Q(QH)2](3-) (Q = S, Se) in which HQ- is a terminal ligand and Q(2-) is a mu2-bridging atom in the core...
  44. pmc Sex-limited protein: in vitro and in vivo functions
    F J Beurskens
    Eijkman Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Utrecht University Hospital, Utrecht, The Netherlands
    Clin Exp Immunol 116:395-400. 1999
    ..C57Bl/6J mice were tested for functional Slp and classical and alternative complement pathway activities (CH50 and AP50 levels, respectively)...
  45. ncbi Early activation of inflammation and clotting in the preterm lamb with neonatal RDS: comparison of conventional ventilation and high frequency oscillatory ventilation
    A S Jaarsma
    Department of Pediatrics, Division of Neonatology, University Hospital Groningen, The Netherlands
    Pediatr Res 50:650-7. 2001
    ..minutes after birth and at 2-h intervals thereafter blood samples, from umbilical catheters, were analyzed for AP50 (complement activation), number of polymorphonuclear leukocytes, beta-glucuronidase, platelet function, activated ..
  46. ncbi Signalling through phospholipase C interferes with clathrin-mediated endocytosis
    Nicolas Carvou
    Department of Physiology, University College London, London WC1E 6JJ UK
    Cell Signal 19:42-51. 2007
    ..The two uptake mechanisms were different as only insulin uptake was reduced when the mu2-subunit of the adaptor complex AP-2 was silenced by RNA interference...
  47. ncbi A Synechococcus elongatus PCC 7942 mutant with a higher tolerance toward the herbicide bentazone also confers resistance to sodium chloride stress
    Suvendra N Bagchi
    BioVIII Lehrstuhl für Molekulare Zellphysiologie, Fakultat fur Biologie, Universitat Bielefeld, Universitatsstr 25, Bielefeld, 33615, Germany
    Photosynth Res 92:87-101. 2007
    ..Mutant Mu1 has in part been previously characterized. In the present paper we report on another mutant, called Mu2, which also has a higher tolerance toward bentazone...
  48. ncbi Analgesic effects of Tyr-W-MIF-1: a mixed mu2-opioid receptor agonist/mu1-opioid receptor antagonist
    K A Gergen
    Tulane University School of Medicine, New Orleans, LA 70146, USA
    Eur J Pharmacol 316:33-8. 1996
    ..Recently, intrathecal (i.t.) Tyr-W-MIF-1 was shown to induce potent analgesia mediated through spinal mu2-opioid receptors in mice...
  49. pmc Rotavirus and reovirus modulation of the interferon response
    Barbara Sherry
    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA
    J Interferon Cytokine Res 29:559-67. 2009
    ..Reovirus likely inhibits the IFN response by at least 3 virus strain-specific mechanisms. First, the reovirus mu2 protein can induce an unusual nuclear accumulation of IRF9 and repress IFN-stimulated gene (ISG) expression, most ..
  50. ncbi Synthesis, structural characterization, and theoretical studies of gold(I) and gold(I)-gold(III) thiolate complexes: quenching of gold(I) thiolate luminescence
    Manuel Bardají
    Quimica Inorganica, Facultad de Ciencias, Universidad de Valladolid, E 47005 Valladolid, Spain
    Inorg Chem 45:1059-68. 2006
    ..dinuclear or tetranuclear mixed gold(I)-gold(III) derivatives with a thiolate bridge, namely, [(AuPPh3){Au(C6F5)3}(mu2-2-SC6H4NH2)] (4) and [(C6F5)3Au(mu2-2-SC6H4NH2)(AudppmAu)(mu2-2-SC(6)H4NH2)Au(C6F5)3] (5)...
  51. pmc Silencing and complementation of reovirus core protein mu2: functional correlations with mu2-microtubule association and differences between virus- and plasmid-derived mu2
    John Carvalho
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Virology 364:301-16. 2007
    A low-copy component of mammalian reovirus particles is mu2, an 83-kDa protein encoded by the M1 viral genome segment and packaged within the viral core...
  52. ncbi Large tandem duplication associated with a Mu2 insertion in Zea mays B-Peru gene
    L J Harris
    Plant Research Centre, Central Experimental Farm, Agriculture Canada, Ottawa, Ontario
    Plant Mol Biol 25:817-28. 1994
    ..Characterization of relevant regions of b-Perum220 revealed a Mu2 element insertion in one copy of a 534 bp sequence...
  53. ncbi Avian reovirus core protein muA expressed in Escherichia coli possesses both NTPase and RTPase activities
    Yu Pin Su
    Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan
    J Gen Virol 88:1797-805. 2007
    ..acid sequence of core protein muA of avian reovirus has indicated that it may share similar functions to protein mu2 of mammalian reovirus...
  54. pmc Increased ubiquitination and other covariant phenotypes attributed to a strain- and temperature-dependent defect of reovirus core protein mu2
    Cathy L Miller
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave, Boston, MA 02115, USA
    J Virol 78:10291-302. 2004
    ..difference in the morphology of these structures reflects more effective microtubule association by the mu2 core proteins of some viral strains, which form filamentous factories, than by those of others, which form globular ..
  55. ncbi A Tyr-W-MIF-1 analog containing D-Pro2 discriminates among antinociception in mice mediated by different classes of mu-opioid receptors
    Daisuke Nakayama
    Department of Physiology and Anatomy, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Aoba ku, Sendai 981 8558, Japan
    Eur J Pharmacol 563:109-16. 2007
    ..The present results clearly suggest that D-Pro2-Tyr-W-MIF-1 is a selective antagonist for the mu2-opioid receptor in the mouse brain...
  56. ncbi Molecular epidemiology of Candida species isolated from clinical specimens of intensive care unit patients
    Serhat Marol
    Department of Microbiology and Clinical Microbiology, School of Medicine, Dokuz Eylul University, Inciralti, Izmir, Turkey
    Mycoses 51:40-9. 2008
    ..The strains were typed by random amplified polymorphic DNA (RAPD)-PCR using OPE-03, OPE-18, RP4-2 and AP50-1 primers. In the patients with Candida infections, most isolates exhibited different RAPD patterns. Only three C...
  57. ncbi Molecular cloning and complete amino acid sequence of AP50, an assembly protein associated with clathrin-coated vesicles
    C Thurieau
    Universite de Paris V, CNRS No 1188, France
    DNA 7:663-9. 1988
    b>AP50 is the 50,000-dalton protein component found in clathrin-coated vesicles as part of the coat assembly protein (AP) complex, AP-2...
  58. pmc Specificity of interaction between adaptor-complex medium chains and the tyrosine-based sorting motifs of TGN38 and lgp120
    D J Stephens
    Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Biochem J 335:567-72. 1998
    ..Whereas the wild-type version of the cytosolic domain of TGN38 interacts with highest affinity with mu2, we show that the cytosolic domain of lgp120 interacts almost exclusively with mu3A...
  59. ncbi Orthoreovirus and Aquareovirus core proteins: conserved enzymatic surfaces, but not protein-protein interfaces
    Jonghwa Kim
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02115, USA
    Virus Res 101:15-28. 2004
    ..Other evidence indicates that the Orthoreovirus mu2 and Aquareovirus VP5 proteins are homologous, suggesting that VP5 is a core protein as mu2 is known to be...
  60. ncbi Phospholipase C-related inactive protein is implicated in the constitutive internalization of GABAA receptors mediated by clathrin and AP2 adaptor complex
    Takashi Kanematsu
    Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science, and Station for Collaborative Research, Kyushu University, Fukuoka, Japan
    J Neurochem 101:898-905. 2007
    ..The clathrin heavy chain, mu2 and beta2 subunits of AP2 and PRIP-1, were complexed with GABA(A) receptor in brain extract as analyzed by co-..
  61. ncbi Involvement of spinal mu1-opioid receptors on the Tyr-d-Arg-Phe-sarcosine-induced antinociception
    Hirokazu Mizoguchi
    Department of Physiology and Anatomy, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Aoba ku, Sendai 981 8558, Japan
    Eur J Pharmacol 540:67-72. 2006
    ..co-treatment with Tyr-D-Pro-Trp-Phe-NH2 (D-Pro2-endomorphin-1) or Tyr-D-Pro-Trp-Gly-NH2 (D-Pro2-Tyr-W-MIF-1), the mu2-opioid receptor antagonists, did not affect the TAPS-induced antinociception...
  62. ncbi The development of the complement system after 28 weeks' gestation
    B Wolach
    Department of Paediatrics, Meir General Hospital, Sapir Medical Center, Kfar Saba, Israel
    Acta Paediatr 86:523-7. 1997
    ..To assess the levels and development of the various complement components in preterm infants, particularly among those born before 34 weeks' gestation...
  63. ncbi Anti-complement effects of lactoferrin-derived peptides
    Ørjan Samuelsen
    Department of Medical Microbiology, University Hospital of North Norway, P O Box 56, N 9038 Tromsø, Norway
    FEMS Immunol Med Microbiol 41:141-8. 2004
    ..No inhibitory effect of these peptides was observed on the alternative complement pathway in an AP50 assay...
  64. ncbi Gene expression profiling of the early pulmonary response to hyperoxia in mice
    Sandra Perkowski
    Department of Clinical Studies Philadelphia, School of Veterinary Medicine, University of Pennsylvania, 3850 Spruce Street, Philadelphia, PA 19104 6010, USA
    Am J Respir Cell Mol Biol 28:682-96. 2003
    ..a number of other antioxidant enzymes, including glutathione peroxidase, glutathione-S-Transferase (GST) Pi1, GST mu2, and heme oxygenase-1 showing relatively moderate increases...
  65. pmc Inhibition of reovirus by mycophenolic acid is associated with the M1 genome segment
    Laura L Hermann
    Department of Medical Microbiology and Infectious Diseases, University of Manitoba, 730 William Avenue, Winnipeg, Manitoba, Canada R3E 0W3
    J Virol 78:6171-9. 2004
    ..differences in sensitivity to MPA mapped to the viral M1 genome segment, which encodes the minor core protein mu2. MPA also inhibited replication of both strains of reovirus in a variety of other cell lines, including Vero monkey ..
  66. ncbi A Tyr-W-MIF-1 analog containing D-Pro2 acts as a selective mu2-opioid receptor antagonist in the mouse
    Hiroyuki Watanabe
    Department of Physiology and Anatomy, Tohoku Pharmaceutical University, 4 4 1 Komatsushima, Aoba ku, Sendai 981 8558, Japan
    J Pharmacol Exp Ther 312:1075-81. 2005
    ..d-Pro(2)-Tyr-W-MIF-1 may also discriminate between Tyr-W-MIF-1-induced antinociception and the antinociception induced by endomorphin-1 or DAMGO, all of which show a preference for the mu(2)-opioid receptor in the spinal cord...
  67. ncbi Bonding trends of thiosemicarbazones in mononuclear and dinuclear copper(I) complexes: syntheses, structures, and theoretical aspects
    Tarlok S Lobana
    Department of Chemistry, Guru Nanak Dev University, Amritsar, India
    Inorg Chem 45:1535-42. 2006
    ..I (5), Br (6), Cl (7), pyridine-2-carbaldehyde thiosemicarbazone (Hpytsc)], (ii) halogen-bridged dimers, [Cu2(mu2-X)2(eta1-S-Htsc)2(Ph3P)2] [X, Htsc = Br (3), Hbtsc; I (8), furan-2-carbaldehyde thiosemicarbazone (Hftsc); I (11), ..
  68. pmc Recognition of double strand breaks by a mutator protein (MU2) in Drosophila melanogaster
    Raghuvar Dronamraju
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA
    PLoS Genet 5:e1000473. 2009
    ..A mutation, mu2(a), in Drosophila melanogaster decreases the rate of repair of double strand DNA breaks in oocytes, thus leading to ..
  69. ncbi Defective expression of the mu3 subunit of the AP-3 adaptor complex in the Drosophila pigmentation mutant carmine
    C Mullins
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Gen Genet 262:401-12. 1999
    ..sequence tag (EST) database identified orthologs of family members mammalian medium (mu) chain families mu1, mu2, and mu3, of the corresponding AP complexes, and delta-COP, the analogous component of the coatomer (COPI) complex...
  70. ncbi Insights into crystallization mechanism: a synchrotron study of polymorphism in a cobalt acetate cluster compound
    Jonathan C Burley
    University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, England
    Acta Crystallogr C 62:m63-6. 2006
    The structure of the title compound, di-mu3-acetato-1kappa2O,O':2kappaO';2kappaO:3kappa2O,O'-di-mu2-acetato-1kappaO:2kappaO';2kappaO:3kappaO'-octapyridyl-1kappa3N,2kappa2N,3kappa3N-tricobalt(II) bis(hexafluorophosphate), [Co3(C2H3O2)4(..
  71. ncbi Metal-organic frameworks from homometallic chains of nickel(II) and 1,4-cyclohexanedicarboxylate connectors: ferrimagnet-ferromagnet transformation
    Mohamedally Kurmoo
    Laboratoire de Chimie de Coordination Organique, UMR7140 CNRS, Institut Le Bel, Universite Louis Pasteur, Strasbourg, France
    Inorg Chem 45:1627-37. 2006
    ..2H2O (1), [Ni(3)(mu3-OH)2(mu4-trans-1,4-chdc)2(H2O)4].4H2O (2), and [Ni(H2O)4(mu2-trans-1,4-chdc)], depending on the reaction conditions...
  72. ncbi MIL-96, a porous aluminum trimesate 3D structure constructed from a hexagonal network of 18-membered rings and mu3-oxo-centered trinuclear units
    Thierry Loiseau
    Institut Lavoisier UMR CNRS 8180, Institut Universitaire de France, Porous Solids Group, Tectospin, Universite de Versailles Saint Quentin en Yvelines, 45, Avenue des Etats Unis, 78035 Versailles, France
    J Am Chem Soc 128:10223-30. 2006
    ..to each other through the trimesate species, which induce corrugated chains of aluminum octahedra, linked via mu2-hydroxo bonds with the specific -cis-cis-trans- sequence. The 3D framework of MIL-96 reveals three types of cages...
  73. doi Zoledronate complexes. I. Poly[[mu2-aqua[mu3-1-hydroxy-2-(1H,3H-imidazol-3-ium-1-yl)ethylidenediphosphonato]potassium(I)] monohydrate]
    Eleonora Freire
    Gerencia de Investigación y Aplicaciones, Centro Atomico Constituyentes, Comision Nacional de Energia Atomica, Buenos Aires, Argentina
    Acta Crystallogr C 66:m13-6. 2010
    ..Intra- and interplanar interactions are enhanced by a variety of hydrogen bonds involving all available O-H and N-H donors. A strong imidazole-phosphonate C-H...O interaction is present in the structure...
  74. pmc Dimerization Mediated by a Divergent Forkhead-associated Domain Is Essential for the DNA Damage and Spindle Functions of Fission Yeast Mdb1
    Shukun Luo
    From the National Institute of Biological Sciences, Beijing 102206, China and
    J Biol Chem 290:21054-66. 2015
    ..The FHA domain of the Drosophila homolog of MDC1, MU2, also forms a homodimer but utilizes a different dimer interface...
  75. ncbi Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu2
    R C Aguilar
    Cell Biology and Metabolism Branch, NICHD, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    J Biol Chem 272:27160-6. 1997
    ..The medium chains of these complexes, mu1 and mu2, have been implicated in two types of interaction: assembly with the beta1 and beta2 chains of the corresponding ..
  76. ncbi [Electronic structure of the high symmetrical compounds of f-element. Part 33. A novel experimental crystallized trigonal-bipyramidal coordinated lanthanide(III) systems: Pr[N(SiMe3)2](CNR)2 (R = tBu, C6H11)]
    Hanns Dieter Amberger
    Institut für Anorganische und Angewandte Chemie der Universität Hamburg, Germany
    Spectrochim Acta A Mol Biomol Spectrosc 58:379-96. 2002
    ..the low energy range is proved by the excellent agreement of calculated and experimental temperature dependence of mu2(eff) for compound 1...
  77. ncbi Polymeric aqua(mu4-dihydrogen benzene-1,2,4,5-tetracarboxylato)(1,10-phenanthroline)cobalt(II)
    Mao Lin Hu
    Department of Chemistry and Material Science, Wenzhou Normal College, Wenzhou 325027, People s Republic of China
    Acta Crystallogr C 60:m112-3. 2004
    ..The bridging H2TCB2- anions have two coordination modes, viz. mu2-H2TCB2- and mu4-H2TCB2-, resulting in a two-dimensional coordination polymer...
  78. ncbi An EPR method for probing surface magnetic fields, dipolar distances, and magnetization fluctuations in single molecule magnets
    B Rakvin
    Department of Physical Chemistry, Ruder Boskovic Institute, Bijenicka 54, P O Box 180, Zagreb 10002, Croatia
    Spectrochim Acta A Mol Biomol Spectrosc 60:1241-5. 2004
    ..effective magnetic field B on the surface of crystals of the single molecule magnet (SMM) [(C6H15N3)6Fe8(mu3-O)2(mu2-OH)12]Br7(H2O)Br.H2O, (Fe8Br8)...
  79. ncbi Di-u-hydroxo-bis{(acetonitrile)dibenzyl(6-methyl-2-pyridylmethyl)-amine-kappa2N,N]copper(II)} bis(perchlorate)
    Dario Rojas
    Departamento de Química Básica, Facultad de Ciencias Fisicas y Matematicas, Universidad de Chile, Avenida Tupper 2009, Santiago, Chile
    Acta Crystallogr C 61:m115-6. 2005
    ..title compound corresponds to a copper(II) dimer, [Cu2(OH)2(C2H3N)2(C21H22N2)2](ClO4)2, where the metal centres are mu2-bridged by hydroxo groups...
  80. ncbi Coordination polymers formed by bridging 2-substituted tetrazole ligands: poly[[dichlorocopper(II)]-di-mu2-2-propyl-2H-tetrazole-kappa2N1:N4] and poly[[dichlorocopper(II)]-di-mu2-2-allyl-2H-tetrazole-kappa2N1:N4]
    Alexander S Lyakhov
    Physico Chemical Research Institute, Belarusian State University, Leningradskaya Street 14, Minsk 220050, Belarus
    Acta Crystallogr C 61:m158-60. 2005
    ..The Cl atoms do not participate in the polymeric layer formation...
  81. ncbi Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors
    Stephen J Royle
    Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK
    J Cell Sci 118:3073-80. 2005
    ..The crystal structure of a complex between residues 160-435 of the mu2 subunit of AP2 and a P2X4 C-terminal peptide showed that the YEQGL motif binds to mu2 at the same site as YXXPhi ..
  82. pmc Mechanism for removal of tumor necrosis factor receptor 1 from the cell surface by the adenovirus RIDalpha/beta complex
    Y Rebecca Chin
    Department of Microbiology and Immunology, Forchheimer Building, Room 411, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Virol 79:13606-17. 2005
    ..By using small interfering RNA against the mu2 subunit of adaptor protein 2, dominant negative dynamin construct K44A, and the lysosomotropic agents bafilomycin ..
  83. ncbi [Anticholinergic effect of procainamide and its sulfonylcarbamide derivatives on electromechanical activity in guinea pig atrium]
    Herta Gurskaite
    Institute of Cardiology, Kaunas University of Medicine, Kaunas, Lithuania
    Medicina (Kaunas) 41:1054-60. 2005
    ..perfusion of atrial strips with Tyrode solution), mean action potential duration measured at 90% (AP90) and 50% (AP50) of repolarization were 92.5+/-4.5 ms and 44.4+/-2.9 ms (n=18), respectively, and contraction force was of 2.7+/-0...
  84. ncbi Complementarity and countercomplementarity in polynuclear copper(II) complexes with R2NCH2CH(OH)CH2NR2 (R = H, CH3): crystal structures and magnetic study
    M Salah El Fallah
    Departament de Quimica Inorganica, Universitat de Barcelona, Marti i Franques 1 11, 08028, Barcelona, Spain
    Dalton Trans . 2006
    ..structural characterization and magnetic behavior of five new copper(II) polynuclear compounds with formulae [Cu4(mu2-CH3COO)2(mu-bdmap)2(micro(1,5)-dca)2(dca)2(H2O)2] 1, [Cu2(mu2-CH3COO)(mu-bdap)(mu(1,1,5)-dca)(mu(1,3)-dca)]n 2, [..
  85. ncbi Ferromagnetic vs. antiferromagnetic coupling in bis(mu2-1,1-azido)dinickel(II) complexes with syn- and anti-conformations of the end-on azide bridges
    Phalguni Chaudhuri
    Max Planck Institut fur Bioanorganische Chemie, Stiftstrasse 34 36, D 45470, Mulheim an der Ruhr, Germany
    Dalton Trans . 2006
    ..The magnetic behaviours of these compounds are discussed in the context of the known bis(end-on azido) bridging dinickel(II) complexes...
  86. ncbi Kit for unsymmetric dinucleating double-Schiff-base ligands: facile access to a versatile new ligand system and its first heterobimetallic copper-zinc complex
    Arne Roth
    Institut fur Anorganische und Analytische Chemie, Friedrich Schiller Universitat Jena, Carl Zeiss Promenade 10, D 07745 Jena, Germany
    Inorg Chem 46:4362-4. 2007
    ..The reaction of the ligand H2bpamptsc with zinc(II) acetate and copper(II) perchlorate yields the heterobinuclear Cu-Zn complex [CuZn(bpamptsc)(mu2,eta1-OAc)(MeCN)](ClO4) (1).
  87. pmc beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53
    Cedric Boularan
    Institut Cochin, Universite Paris Descartes, Centre National de la Recherche Scientifique Unite Mixte de Recherche 8104, 75014 Paris, France
    Proc Natl Acad Sci U S A 104:18061-6. 2007
    ..These observations suggest that the intracellular concentration of beta-arr2 oligomers might control cell survival and proliferation...
  88. doi C-H bond activation by air-stable [(diimine)M(II)(mu2-OH)]2(2+) dimers (M = Pd, Pt)
    TRAVIS J WILLIAMS
    Arnold and Mabel Beckman Laboratories of Chemical Synthesis, California Institute of Technology, Pasadena, California 91125, USA
    J Am Chem Soc 130:2418-9. 2008
  89. pmc Molecular characterization of a variant of Bacillus anthracis-specific phage AP50 with improved bacteriolytic activity
    Shanmuga Sozhamannan
    Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Appl Environ Microbiol 74:6792-6. 2008
    ..Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype...
  90. pmc The dyslexia-associated protein KIAA0319 interacts with adaptor protein 2 and follows the classical clathrin-mediated endocytosis pathway
    Clotilde Levecque
    Wellcome Trust Centre for Human Genetics, Univ of Oxford, Roosevelt D, Oxford OX3 7BN, UK
    Am J Physiol Cell Physiol 297:C160-8. 2009
    ..The present study has identified the medium subunit (mu2) of adaptor protein 2 (AP-2) as a binding partner of KIAA0319 in a yeast two-hybrid screen...
  91. doi Essential and supporting host cell factors for HIV-1 budding
    Aurelien Dordor
    Unit of Virus Host Cell Interactions UMI 3265 Université Joseph Fourier EMBL CNRS, 6 rue Jules Horowitz 38042 Grenoble Cedex 9, France
    Future Microbiol 6:1159-70. 2011
    ..Here we review recent advances in understanding the role of host cell factors recruited by Gag during HIV-1 assembly and budding...
  92. doi Structure analysis of a heteropolysaccharide from Taraxacum mongolicum Hand.-Mazz. and anticomplementary activity of its sulfated derivatives
    Miaomiao Chen
    The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China
    Carbohydr Polym 152:241-52. 2016
    ..94±0.43μg/mL; heparin, 104.40±3.82μg/mL) and alternative pathway (AP50: Sul-DPSW-A, 42.76±0.46μg/mL; heparin, 43.42±0.22μg/mL)...
  93. pmc Identification of the phoM gene product and its regulation in Escherichia coli K-12
    D Ludtke
    J Bacteriol 159:19-25. 1984
    ..fusion was constructed by using a plasmid carrying the phoM gene and a derivative of phage lambda, lambda plac Mu2. Restriction endonuclease analysis of the plasmid carrying the fusion indicated that phoM is transcribed in a ..
  94. ncbi Serine 331 and tyrosine 333 are both involved in the interaction between the cytosolic domain of TGN38 and the mu2 subunit of the AP2 clathrin adaptor complex
    D J Stephens
    Department of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom
    J Biol Chem 272:14104-9. 1997
    ..It was recently demonstrated that this motif specifically interacts with the mu2 subunit of the AP2 adaptor complex...
  95. ncbi The effects of postmortem delay on mu, delta and kappa opioid receptor subtypes in rat brain and guinea pig cerebellum evaluated by radioligand receptor binding
    D Paul
    Department of Pharmacology, LSU Medical Center, New Orleans, LA 70112, USA
    Life Sci 61:1993-8. 1997
    ..Tissue homogenates from these brains were analysed for mu1, mu2, delta, kappa1 and kappa3 opioid receptor binding using standard radioligand binding techniques...
  96. ncbi Concomitant effect of topical ubiquinone Q10 and vitamin E to prevent keratocyte apoptosis after excimer laser photoablation in rabbits
    Rosario Brancato
    Department of Ophthalmology and Visual Sciences, University Hospital San Raffaele of Milan, Italy
    J Refract Surg 18:135-9. 2002
    ..To investigate in vivo whether ubiquinone Q10 together with vitamin E protects rabbit corneas from keratocyte apoptosis after excimer laser irradiation...
  97. ncbi Polymeric tris(mu2)-acetone-kappa2O:O)sodium polyiodide at 120 K
    R Alan Howie
    Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland, UK
    Acta Crystallogr C 59:m184-6. 2003
    ..Na distances of 2.439 (5) and 3.2237 (4) A, respectively. I atoms form infinite linear chains, in which the I-atom separation is 3.2237 (4) A...
  98. ncbi Differentiation of opioid receptor preference by [Dmt1]endomorphin-2-mediated antinociception in the mouse
    Yunden Jinsmaa
    Medicinal Chemistry Group, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    Eur J Pharmacol 509:37-42. 2005
    ..vivo model paradigms: naloxone (non-specific opioid receptor antagonist) and beta-funaltrexamine (irreversible mu1/mu2-opioid receptor antagonist) blocked antinociception in both tests, although stronger inhibition occurred in the hot-..
  99. ncbi Crystal structures and cytotoxicity of isopropylamine Pt(II) complexes: a trinuclear squarato-bridged [Pt3(mu2-C4O4)3(H2NPri)6].3H2O and a mononuclear cis-[Pt(NO3)2(H2NPri)2]
    Sabina Grabner
    Faculty of Chemistry and Chemical Technology, Department of Chemistry, University of Ljubljana, Slovenia
    J Inorg Biochem 99:1465-71. 2005
    ....
  100. ncbi Tetrapiperidinium di-mu-methoxy-di-mu5-oxo-tetra-mu3-oxo-dodeca-mu2-oxo-octaoxodecavanadate
    Fen Sun
    Marine Drug and Food Institute, Ocean University of China, 266003 Qingdao, People s Republic of China
    Acta Crystallogr C 62:m60-2. 2006
    ..The compound is distinguished by presenting, in contrast with other anionic decavanadates, two methoxy groups bridging the outermost V atoms, and it becomes the first example of this type among reported decavanadates...
  101. ncbi Synthesis and characterization of f-element iodate architectures with variable dimensionality, alpha- and beta-Am(IO3)3
    Wolfgang Runde
    Chemistry and Nuclear Materials Technology Divisions, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA
    Inorg Chem 45:2479-82. 2006
    ..IO3] ligands are solely corner-sharing with [AmO8] polyhedra, a complex arrangement of corner- and edge-sharing mu2- and mu3-[IO3] pyramids can be found in beta-Am(IO3)3...

Research Grants18

  1. Martina Buck; Fiscal Year: 2014
    ..Clathrin-mediated endocytosis (CME), through site-specific phosphorylations of <2(AP50), a subunit of the heterodimeric cargo adaptor AP2, a key regulator of clathrin-mediated receptor endocytosis and ..
  2. HEPATOCELLULAR CARCINOMA AND HEPATITIS C: E2 MOLECULAR MECHANISMS
    Martina Buck; Fiscal Year: 2010
    ..Clathrin-mediated endocytosis, through a site-specific phosphorylation of the clathrin adaptor protein-50 (AP50), a key regulator of clathrin-mediated receptor endocytosis;and 2...
  3. Characterizing exon 11 promoter of the mu opioid receptor gene, OPRM
    Ying Xian Pan; Fiscal Year: 2012
    ..Early pharmacological studies have proposed several mu opioid receptor subtypes: mu1, mu2 and morphine-62-glucuronide (M6G)...
  4. Intracellular mechanisms of reovirus genome replication and particle assembly
    MAX NIBERT; Fiscal Year: 2007
    ..are proposed to be studied insofar as they associate with muNS, with a special emphasis on the viral sigmaNS and mu2 proteins, which have also been implicated in determining features or functions of the factories...
  5. Arrestins in G protein-coupled receptor trafficking
    GREGORY FRALISH; Fiscal Year: 2005
    ..We have recently discovered that a small region of the N-terminus of beta-arrestin2 binds to the mu2-adaptin subunit of AP2...
  6. Research Training in Viral mu2 Localization and Function
    John Carvalho; Fiscal Year: 2006
    ..The mu2 protein, an inner capsid component of reovirus, is suggested to be a regulator of viral cytopathology...
  7. CD5 MODULATION AND REGULATION OF CK2 IN B CELL FUNCTION
    Chander Raman; Fiscal Year: 1999
    ..The investigator has found that the cytoplasmic tail of CD5 interacts with the AP50 subunit of the AP2-adaptor complex that links cytoplasmic tails of membrane proteins to clathrin-coated pits...
  8. MU-OPIOID AND ALPHA2-ADRENERGIC MECHANISMS IN ANALGESIA
    ANNA LEENA SIREN; Fiscal Year: 1993
    The primary objective of the proposed research is to test the hypothesis that a alpha2-adrenergic-mu2-opioid (alpha2/mu2) receptor complex mediates the respiratory depression and development of physical dependence associated with opiate ..
  9. MOLECULAR MECHANISMS MEDIATING CD22 ACCESSORY FUNCTION
    LOUIS JUSTEMENT; Fiscal Year: 2003
    ..Additionally, studies performed in this laboratory have determined that CD22 physically interacts with AP50, the medium chain subunit of the AP-2 complex, via a tyrosine-containing motif...
  10. MOLECULAR MECHANISMS IN REOVIRUS TRANSCRIPTION & CAPPING
    MAX NIBERT; Fiscal Year: 2000
    ..The general approach involves expression of the five core proteins (gamma1, gamma2, gamma3, mu2, and sigma2) for studies of their structure, function, and assembly into particles and more classical genetic and ..
  11. Stoichiometry and modular retrieval of ENaC
    James Stockand; Fiscal Year: 2009
    ..the channel for ubiquitinylation via Nedd4 ubiquitin ligases;and the latter motif is known to interact with the mu2 subunit of the AP-2 complex, which targets proteins for clathrin coated-pit endocytosis mediated by dynamin...
  12. ANNEXIN PHOSPHORYLATION: CAUSES AND CONSEQUENCES
    CARL CREUTZ; Fiscal Year: 2004
    ....
  13. Research Training in Virus RNA Localization
    Cathy Miller; Fiscal Year: 2004
    ..Understanding the RNA localization process may lead to the development of new anti-viral strategies, and will aid in the development of a robust reverse genetics system for dsRNA viruses. ..
  14. Poxvirus adverse effects on cardiac cells and the heart
    Barbara Sherry; Fiscal Year: 2005
    ..Studies proposed in this R21 application will provide the foundation for future in-depth studies with the goal of improving current and future smallpox vaccines. ..
  15. The cardiac interferon response to reovirus infection
    Barbara Sherry; Fiscal Year: 2008
    ..In sum, results will identify cell type-specific IFN-beta-related responses critical for protection against myocarditis, potentially providing new avenues for intervention against viral infections of the heart. ..
  16. CALCIUM DEPENDENT MEMBRANE BINDING PROTEINS
    CARL CREUTZ; Fiscal Year: 2002
    ....
  17. ACUTE MYOCARDITIS--ROLES OF REOVIRUS AND INTERFERON BETA
    Barbara Sherry; Fiscal Year: 2001
    ..Reoviruses offer a unique tool to investigate the IFN response invoked by closely related viruses in the heart. Our work will be the first to identity cell factors that are determinants of this cardiac IFN response and myocarditis. ..
  18. The role of reovirus factories in viral RNA regulation.
    Cathy Miller; Fiscal Year: 2007
    ..unreadable] [unreadable]..