alpha2 3 sialyltransferase

Summary

Gene Symbol: alpha2 3 sialyltransferase
Description: ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Alias: SIAT10, ST3GALVI, type 2 lactosamine alpha-2,3-sialyltransferase, CMP-NeuAc:beta-galactoside alpha-2,3-sialyltransferase VI, alpha2,3-sialyltransferase ST3Gal VI, sialyltransferase 10 (alpha-2,3-sialyltransferase VI)
Species: human

Top Publications

  1. ncbi Expression and characterization of human N-acetylglucosaminyltransferases and alpha2,3-sialyltransferase in insect cells for in vitro glycosylation of recombinant erythropoietin
    Na Young Kim
    Department of Life Science, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 18:383-91. 2008
  2. ncbi Generation of serum-stabilized retroviruses: reduction of alpha1,3gal-epitope synthesis in a murine NIH3T3-derived packaging cell line by expression of chimeric glycosyltransferases
    Wiebke Hansen
    Department of Regulation and Differentiation, Molecular Biotechnology, German Research Centre of Biotechnology, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Metab Eng 7:221-8. 2005
  3. doi Alpha2,3-sialyltransferase III knockdown sensitized ovarian cancer cells to cisplatin-induced apoptosis
    Xiaoyu Wang
    Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, 510630, Guangzhou, China Electronic address
    Biochem Biophys Res Commun 482:758-763. 2017
  4. doi Cigarette smoking alters sialylation in the Fallopian tube of women, with implications for the pathogenesis of ectopic pregnancy
    Junko Nio-Kobayashi
    Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Mol Reprod Dev 83:1083-1091. 2016
  5. doi Functions of Smad Transcription Factors in TGF-β1-Induced Selectin Ligand Expression on Murine CD4 Th Cells
    Mark E Ebel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611
    J Immunol 197:2627-34. 2016
  6. pmc Targeting Selectins and Their Ligands in Cancer
    Alessandro Natoni
    Biomedical Sciences, National University of Ireland Galway, Galway, Ireland
    Front Oncol 6:93. 2016
  7. pmc Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes
    Benjamin L Parker
    From the Charles Perkins Centre, School of Molecular Bioscience and
    Mol Cell Proteomics 15:141-53. 2016
  8. pmc Characterization of the platelet transcriptome by RNA sequencing in patients with acute myocardial infarction
    John D Eicher
    a The Framingham Heart Study, Framingham, MA, USA
    Platelets 27:230-9. 2016
  9. doi Assessment of the coordinated role of ST3GAL3, ST3GAL4 and ST3GAL6 on the α2,3 sialylation linkage of mammalian glycoproteins
    Cheng Yu Chung
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 463:211-5. 2015
  10. pmc Diverse inflammatory cytokines induce selectin ligand expression on murine CD4 T cells via p38α MAPK
    Mark E Ebel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611
    J Immunol 194:5781-8. 2015

Research Grants

Scientific Experts

Detail Information

Publications92

  1. ncbi Expression and characterization of human N-acetylglucosaminyltransferases and alpha2,3-sialyltransferase in insect cells for in vitro glycosylation of recombinant erythropoietin
    Na Young Kim
    Department of Life Science, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 18:383-91. 2008
    ..A significant increase in the number of reticulocytes was not observed in the mice injected with in vitro glycosylated rhEPO as compared with those injected with rhEPO...
  2. ncbi Generation of serum-stabilized retroviruses: reduction of alpha1,3gal-epitope synthesis in a murine NIH3T3-derived packaging cell line by expression of chimeric glycosyltransferases
    Wiebke Hansen
    Department of Regulation and Differentiation, Molecular Biotechnology, German Research Centre of Biotechnology, Mascheroder Weg 1, D 38124 Braunschweig, Germany
    Metab Eng 7:221-8. 2005
    ....
  3. doi Alpha2,3-sialyltransferase III knockdown sensitized ovarian cancer cells to cisplatin-induced apoptosis
    Xiaoyu Wang
    Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, 510630, Guangzhou, China Electronic address
    Biochem Biophys Res Commun 482:758-763. 2017
    ..Taken together, these results uncover an alternative mechanism of cisplatin-resistance through ST3Gal3 and open a window for effective prevention of chemoresistance and relapse of ovarian cancer by targeting ST3Gal3...
  4. doi Cigarette smoking alters sialylation in the Fallopian tube of women, with implications for the pathogenesis of ectopic pregnancy
    Junko Nio-Kobayashi
    Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Mol Reprod Dev 83:1083-1091. 2016
    ..Mol. Reprod. Dev. 83: 1083-1091, 2016. © 2016 Wiley Periodicals, Inc...
  5. doi Functions of Smad Transcription Factors in TGF-β1-Induced Selectin Ligand Expression on Murine CD4 Th Cells
    Mark E Ebel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611
    J Immunol 197:2627-34. 2016
    ..These results show that Smads are directly required for Th cell differentiation independent of Runx induction but only indirectly required via Runx2 for TGF-β1-induced selectin ligand induction on murine CD4 T cells. ..
  6. pmc Targeting Selectins and Their Ligands in Cancer
    Alessandro Natoni
    Biomedical Sciences, National University of Ireland Galway, Galway, Ireland
    Front Oncol 6:93. 2016
    ....
  7. pmc Terminal Galactosylation and Sialylation Switching on Membrane Glycoproteins upon TNF-Alpha-Induced Insulin Resistance in Adipocytes
    Benjamin L Parker
    From the Charles Perkins Centre, School of Molecular Bioscience and
    Mol Cell Proteomics 15:141-53. 2016
    ..This revealed the modulation of N-glycosylation on specific proteins in IR, including those previously associated with insulin-stimulated GLUT4 trafficking to the plasma membrane. ..
  8. pmc Characterization of the platelet transcriptome by RNA sequencing in patients with acute myocardial infarction
    John D Eicher
    a The Framingham Heart Study, Framingham, MA, USA
    Platelets 27:230-9. 2016
    ..Future platelet RNA-seq studies examining more diverse sets of healthy and diseased samples will add to our understanding of platelet thrombotic and non-thrombotic functions...
  9. doi Assessment of the coordinated role of ST3GAL3, ST3GAL4 and ST3GAL6 on the α2,3 sialylation linkage of mammalian glycoproteins
    Cheng Yu Chung
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Biochem Biophys Res Commun 463:211-5. 2015
    ..From these experiments, we concluded that each of these three genes were involved in N-linked sialylation and ST3GAL4 may play the critical role in glycoprotein sialylation of recombinant proteins such as EPO. ..
  10. pmc Diverse inflammatory cytokines induce selectin ligand expression on murine CD4 T cells via p38α MAPK
    Mark E Ebel
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611
    J Immunol 194:5781-8. 2015
    ..Our results identify the repertoire of cytokines responsible for selectin ligand induction on CD4 T cells and provide a mechanistic link between Th cell development and T cell migration...
  11. pmc alpha2,3-sialyltransferase ST3Gal III modulates pancreatic cancer cell motility and adhesion in vitro and enhances its metastatic potential in vivo
    Marta Pérez-Garay
    Biochemistry and Molecular Biology Unit, Department of Biology, University of Girona, Girona, Spain
    PLoS ONE 5:. 2010
    ..We have focused on the influence of alpha-2,3-sialyltransferase ST3Gal III in key steps of the pancreatic tumorigenic process...
  12. doi Differential expression of alpha-2,3-sialyltransferases and alpha-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells
    A S Carvalho
    Instituto de Patologia e Imunologia Molecular da Universidade do Porto IPATIMUP, Porto, Portugal
    Int J Biochem Cell Biol 42:80-9. 2010
    ..This increase was observed together with an increase in mRNA levels of ST3GAL3, FUT5 and FUT6, and a decrease in FUT4 transcript levels in MKN45 confluent cells, suggesting a possible control at the transcriptional level...
  13. ncbi Chronic ethanol consumption down-regulates CMP-NeuAc:GM3 alpha 2,8-sialyltransferase (ST8Sia-1) gene in the rat brain
    Mamatha Garige
    The Lipid Research Laboratory, 151 T, Veterans Affairs Medical Center, 50 Irving Street NW, Washington, DC 20422, USA
    Neurochem Int 49:312-8. 2006
    ..Therefore, the selective loss of 2,8-sialic acid residues from gangliosides might contribute towards the appearance of asialogangliosides and related brain-abnormalities associated with ethanol abuse...
  14. ncbi Sensitivity to human serum of gammaretroviruses produced from pig endothelial cells transduced with glycosyltransferase genes
    Takashi Kurihara
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Xenotransplantation 10:562-8. 2003
    ..Our results suggest that gammaretroviruses including PERVs produced by transgenic pigs, that are generally modified to reduce the cell-surface alpha-Gal epitope to overcome the HAR in xenotransplantation, are less sensitive to HS...
  15. ncbi Validation of the Hsp150 polypeptide carrier and HSP150 promoter in expression of rat alpha2,3-sialyltransferase in yeasts
    Eeva Sievi
    Program in Cellular Biotechnology, Institute of Biotechnology, and Department of Applied Chemistry and Microbiology, University of Helsinki, Viikinkaari 9, 00710 Helsinki, Finland
    Biotechnol Prog 19:1368-71. 2003
    ..Most of Hsp150Delta-ST3Ne and MFalpha-ST3Ne remained noncovalently attached to the cell wall via the ST3Ne portion. The strength of the HSP150 promoter was found to be comparable to that of the GAL1 promoter...
  16. ncbi Alpha2,6-sialylation of cell-surface N-glycans inhibits glioma formation in vivo
    H Yamamoto
    Chicago Institute for Neurosurgery and Neuroresearch, Chicago, Illinois 60614, USA
    Cancer Res 61:6822-9. 2001
    ..Thus, manipulating ST6Gal I gene expression may have therapeutic potential for the treatment of malignant gliomas...
  17. ncbi Increased alpha2,6 sialylation of N-glycans in a transgenic mouse model of hepatocellular carcinoma
    D Pousset
    Glycobiologie, Centre de Biophysique Moleculaire, Orleans, France
    Cancer Res 57:4249-56. 1997
    ..The single transcripts detected in both normal and cancerous liver showed identical size...
  18. ncbi Gene-expression profiles for five key glycosylation genes for galactose-fed CHO cells expressing recombinant IL-4/13 cytokine trap
    Kevin J R Clark
    Department of Chemical Engineering, 112 Biosystems Research Complex, Clemson University, Clemson, South Carolina 29634 0905, USA
    Biotechnol Bioeng 90:568-77. 2005
    ..Thus, galactose feeding is an unnecessary expense for the production of the IL-4/13 cytokine trap fusion protein in a batch process...
  19. ncbi Expression of the high-affinity selectin glycan ligand C2-O-sLeX by colon carcinoma cells
    Catherine A St Hill
    Department of Veterinary and Biomedical Sciences, University of Minnesota Twin Cities campuses, Minneapolis St Paul, MN 55108, USA
    Cancer Lett 217:105-13. 2005
    ..Hence, we demonstrate for the first time the detection of C2-O-sLeX on colon carcinoma cells, which, as with leukocytes, may be directly relevant to the expression of high affinity glycan ligands for the selectins...
  20. ncbi Production of a complement inhibitor possessing sialyl Lewis X moieties by in vitro glycosylation technology
    Lawrence J Thomas
    AVANT Immunotherapeutics, 119 Fourth Ave, Needham, MA 02494, USA
    Glycobiology 14:883-93. 2004
    ..These results demonstrate that in vitro glycosylation of the sCR1 drug product reduces heterogeneity of the glycan profile, improves pharmacokinetics, and enhances carbohydrate-mediated binding to E-selectin...
  21. ncbi A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria
    Bernard Priem
    Centre de Recherches sur les Macromolecules Vegetales, CNRS, Joseph Fourier University, BP 53, 38041 Grenoble cedex 9 France
    Glycobiology 12:235-40. 2002
    ..meningitidis genes of the alpha2,3 sialyltransferase and of the CMP-NeuAc synthase efficiently produced sialyllactose (NeuAc alpha 2-3Gal beta 1-4Glc) from exogenous NeuAc and lactose...
  22. ncbi Alteration of sialyl Lewis epitope expression in pterygium
    C Creuzot-Garcher
    Department of Ophthalmology, University of Burgundy, Dijon, France
    Invest Ophthalmol Vis Sci 40:1631-6. 1999
    ..The pattern of these antigens in pterygium, and especially the role of Galbeta1-3GlcNAc alpha2,3-sialyltransferase (ST3Gal III), sialyltransferase necessary to build the sialyl-Le(a) (Lewis(a)) antigen, were studied...
  23. pmc Relationship of sialyl-Lewis(x/a) underexpression and E-cadherin overexpression in the lymphovascular embolus of inflammatory breast carcinoma
    Mary L Alpaugh
    Department of Pathology, University of California at Los Angeles School of Medicine, Los Angeles, California 90024, USA
    Am J Pathol 161:619-28. 2002
    ..Our findings support the cooperative relationship of sLe(x/a) underexpression and E-cadherin overexpression in the genesis of the lymphovascular embolus of IBC...
  24. ncbi The Hexapeptide inhibitor of Galbeta 1,3GalNAc-specific alpha 2,3-sialyltransferase as a generic inhibitor of sialyltransferases
    Ki Young Lee
    Animal Cell and Medical Glycobiology Laboratory and Liver Cell Signal Transduction Laboratory, Korea Research Institute of Bioscience and Biotechnology, P O Box 115, Yusung, Taejon 305 333, South Korea
    J Biol Chem 277:49341-51. 2002
    ....
  25. ncbi Potent and specific sialyltransferase inhibitors: imino-linked 5a'-carbadisaccharides
    Kensuke Okazaki
    Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Hiyoshi, Kohoku Ku, Yokohama, 223 8522, Japan
    Org Biomol Chem 1:2229-30. 2003
    ..Methyl 5a'-carba-beta-lactoside, imino-linked, has been shown to possess potent and specific inhibitory activity (IC50 = 185 microM) toward rat recombinant alpha2,3-sialyltransferase...
  26. ncbi Apoptosis of human breast carcinoma cells in the presence of cis-platin and L-/D-PPMP: IV. Modulation of replication complexes and glycolipid: Glycosyltransferases
    Patrick J Boyle
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
    Glycoconj J 23:175-87. 2006
    ....
  27. ncbi A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries
    Hai Yu
    Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA
    J Am Chem Soc 127:17618-9. 2005
    ..The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality...
  28. ncbi Spatiotemporal expression patterns of sialoglycoconjugates during nephron morphogenesis and their regional and cell type-specific distribution in adult rat kidney
    Christian Zuber
    Division of Cell and Molecular Pathology, Department of Pathology, University of Zurich, Schmelzbergstrasse 12, 8091 Zurich, Switzerland
    Histochem Cell Biol 120:143-60. 2003
    ..Thus, alpha2,6- and alpha2,3-linked sialic acids are differently expressed in embryonic anlagen and mesenchyme-derived early stages of nephron and show regional and cell type-specific differences in adult kidney...
  29. ncbi High-level expression of human glycosyltransferases in insect cells as biochemically active form
    Hyung Gu Kim
    Department of Life Science, Sogang University, Mapo gu, Seoul 121 742, Republic of Korea
    Biochem Biophys Res Commun 305:488-93. 2003
    ..Taken together, the mouse IgM signal peptide and IgG binding domain of the protein A could be efficiently used as an N-terminus fusion partner for the over-expression of heterologous proteins in insect cells...
  30. pmc A fine-mapping study of 7 top scoring genes from a GWAS for major depressive disorder
    Eva C Verbeek
    Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands
    PLoS ONE 7:e37384. 2012
    ..9E-7 but was not genome wide significant. Although our study did not identify a more strongly associated variant, the results for PCLO suggest that the causal variant is in high LD with rs2715147, rs2715148 and rs2522833...
  31. pmc Coordinated roles of ST3Gal-VI and ST3Gal-IV sialyltransferases in the synthesis of selectin ligands
    Won Ho Yang
    Center for Nanomedicine, Sanford Burnham Medical Research Institute, University of California, Santa Barbara, Santa Barbara, CA 93106, USA
    Blood 120:1015-26. 2012
    ..The results of the present study demonstrate the coordinated participation of both ST3Gal-VI and ST3Gal-IV in the synthesis of functional selectin ligands...
  32. ncbi Effect of mild-thiol reducing agents and alpha2,3-sialyltransferase expression on secretion and sialylation of recombinant EPO in CHO cells
    Kern Hee Chang
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Microbiol Biotechnol 23:699-706. 2013
    ..Thus, our study is significant in that it offers increased EPO production while still allowing the prevention of decreased sialylation of EPO...
  33. ncbi Tumor necrosis factor alpha increases the expression of glycosyltransferases and sulfotransferases responsible for the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa
    Philippe Delmotte
    INSERM U 377 and Université de Lille 2, 59045 Lille Cedex, France
    J Biol Chem 277:424-31. 2002
    ..However, other cytokines may also be involved in this process...
  34. doi Screening for differentially methylated genes among human colorectal cancer tissues and normal mucosa by microarray chip
    Wei Chen
    Department of Colorectal Surgery, the Sixth Affiliated Hospital, Sun Yat Sen University, 26 Yuancunerheng Rd, Guangzhou, Guangdong 510655, People s Republic of China
    Mol Biol Rep 40:3457-64. 2013
    ..Through Preliminary validation, there were two genes (St3gal6, Opcml) in thirty top-ranking genes shown hypermethylated in six pairs of CRC and adjacent normal mucosa...
  35. ncbi Down-regulation of human Galbeta1,3GalNAc/Galbeta1,4GlcNAc alpha2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line
    A Taniguchi
    Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan
    Mol Cell Biol Res Commun 2:197-201. 1999
    ..These results suggest that this element plays a critical role in down-regulating the B3 promoter activity during HL-60 cell differentiation...
  36. ncbi Promoter structure and transcriptional regulation of human beta-galactoside alpha2, 3-sialyltransferase genes
    Akiyoshi Taniguchi
    Biomaterials Center, National Institute for Materials Science 1 1 Namiki, Tsukuba, Ibaraki 305 0044, Japan
    Curr Drug Targets 9:310-6. 2008
    ..The multiple regulatory pathways of these ubiquitous sialyltransferases may be differentially modulated in various cell types...
  37. doi DNA hypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer
    Yuki I Kawamura
    Department of Gastroenterology, Research Institute, International Medical Center of Japan, Tokyo, Japan
    Gastroenterology 135:142-151.e3. 2008
    ..The aim of this study was to clarify the cause of cancer-associated abnormal glycosylation in gastrointestinal (GI) cancers...
  38. pmc Multifunctionality of Campylobacter jejuni sialyltransferase CstII: characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities
    Jiansong Cheng
    Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA
    Glycobiology 18:686-97. 2008
    ....
  39. pmc Sialyltransferase ST3Gal-IV controls CXCR2-mediated firm leukocyte arrest during inflammation
    David Frommhold
    Children s Hospital, University of Heidelberg, 69120 Heidelberg, Germany
    J Exp Med 205:1435-46. 2008
    ..Collectively, we provide substantial evidence that sialylation by ST3Gal-IV significantly contributes to CXCR2-mediated leukocyte adhesion during inflammation in vivo...
  40. doi Enhanced sialylation of recombinant erythropoietin in genetically engineered Chinese-hamster ovary cells
    Yeon Tae Jeong
    Department of Biological Sciences, KAIST Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Appl Biochem 52:283-91. 2009
    ..The present study provides a useful strategy for enhancing the sialylation of therapeutic glycoproteins produced in CHO cells...
  41. ncbi Enhanced sialylation of recombinant erythropoietin in CHO cells by human glycosyltransferase expression
    Yeon Tae Jeong
    Department of Biological Sciences, KAIST, Daejeon 305 701, Korea
    J Microbiol Biotechnol 18:1945-52. 2008
    ..Thus, coexpression of alpha2,3-ST and beta1,4-GT may be beneficial for producing therapeutic glycoproteins with enhanced sialylation in CHO cells...
  42. doi Identification and characterization of flavonoids as sialyltransferase inhibitors
    Kazuya I P J Hidari
    Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Japan and Global COE Program, 52 1 Yada, Suruga ku, Shizuoka 422 8526, Japan
    Biochem Biophys Res Commun 382:609-13. 2009
    ..Kinetic analysis using human ST6Gal I indicated a mixed inhibition mechanism of the compounds. In conclusion, the flavonoids identified could be applied for control of cellular expression of sialic acid...
  43. doi Enhancing of GM3 synthase expression during differentiation of human blood monocytes into macrophages as in vitro model of GM3 accumulation in atherosclerotic lesion
    Elena V Gracheva
    Russian Cardiology Research Center, Ministry of Health of Russian Federation, 3rd Cherepkovskaya Street 15A, 121552, Moscow, Russia
    Mol Cell Biochem 330:121-9. 2009
    ..The presented data suggest that up-regulation of GM3 levels is an element of monocyte/macrophage differentiation that provides a tool for control of macrophage accumulation in inflammatory loci...
  44. pmc Myxoma virus encodes an alpha2,3-sialyltransferase that enhances virulence
    R J Jackson
    Vertebrate Biocontrol CRC, CSIRO Wildlife and Ecology, Canberra, Australia
    J Virol 73:2376-84. 1999
    ..The possible implications of the myxoma virus-expressed sialyltransferase in terms of the host's defenses against infection are discussed...
  45. doi Crystal structure of alpha/beta-galactoside alpha2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum
    Toru Iwatani
    Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka 812 8581, Japan
    FEBS Lett 583:2083-7. 2009
    ..The predicted structure suggested that the broad substrate specificity could be attributed to the wider entrance of the acceptor substrate binding site...
  46. ncbi Expression cloning of mouse cDNA of CMP-NeuAc:Lactosylceramide alpha2,3-sialyltransferase, an enzyme that initiates the synthesis of gangliosides
    S Fukumoto
    Department of Biochemistry II, Nagoya University School of Medicine, 65 Tsurumai, Nagoya 466 0065, Japan
    J Biol Chem 274:9271-6. 1999
    ..Among various mouse tissues, brain, liver, and testis showed relatively high expression of a 2.3-kilobase mRNA, whereas all tissues, more or less, expressed this gene...
  47. doi Polyoxometalates as effective inhibitors for sialyl- and sulfotransferases
    Akira Seko
    Innovative Research Initiatives, Tokyo Institute of Technology, Midori ku, Yokohama, Japan
    J Inorg Biochem 103:1061-6. 2009
    ..Collectively, PMs are novel inhibitors of specific sialyl/sulfotransferases...
  48. doi Selectin ligand sialyl-Lewis x antigen drives metastasis of hormone-dependent breast cancers
    Sylvain Julien
    Breast Cancer Biology, King s College London, Guy s Hospital, London, United Kingdom
    Cancer Res 71:7683-93. 2011
    ....
  49. ncbi Epithelial-cell-specific transcriptional regulation of human Galbeta1,3GalNAc/Galbeta1,4GlcNAc alpha2,3-sialyltransferase (hST3Gal IV) gene
    A Taniguchi
    School of Pharmaceutical Sciences, Toho University, 2 2 1, Miyama, Funabashi, Chiba, 274 8510, Japan
    Biochem Biophys Res Commun 257:516-22. 1999
    ..These results suggest that AP2 plays a critical role in the epithelium-cell specific transcriptional regulation of the hST3Gal IV gene...
  50. doi Sialylation enhancement of CTLA4-Ig fusion protein in Chinese hamster ovary cells by dexamethasone
    Ying Jing
    Biologics Process and Product Development, Technical Operations, Bristol Myers Squibb Company, East Syracuse, New York 13057, USA
    Biotechnol Bioeng 107:488-96. 2010
    ..2% for total sialic acid content and 15.8% in the protein fraction with N-linked sialylation. DEX was found to be a simple and effective method for increasing sialylation of this CTLA4-Ig fusion protein expressed in CHO cells...
  51. pmc Use of genome-wide expression data to mine the "Gray Zone" of GWA studies leads to novel candidate obesity genes
    Jussi Naukkarinen
    FIMM, Institute for Molecular Medicine Finland, University of Helsinki, Finland
    PLoS Genet 6:e1000976. 2010
    ..This study presents a new approach to utilizing gene expression studies for informing choice of candidate genes for complex human phenotypes, such as obesity...
  52. ncbi Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that sialylates type II lactosamine structures on glycoproteins and glycolipids
    T Okajima
    Department of Biochemistry, Nagoya University School of Medicine, Tsurumai, Nagoya 466 0065, Japan
    J Biol Chem 274:11479-86. 1999
    ..Consequently, these data indicated that this enzyme is involved in the synthesis of sialyl-paragloboside, a precursor of sialyl-Lewis X determinant...
  53. ncbi Mutational analysis for enzyme activity of mouse Galbeta1,3GalNAc alpha2,3-sialyltransferase (mST3Gal I)
    Kyoung sook Kim
    Brain Korea 21 Center for Silver Bio Industrialization, Dong A University, Busan 604 714, South Korea
    Indian J Biochem Biophys 47:135-40. 2010
    ..In the case of cysteine mutants, no enzyme activity was observed like tryptophan mutants, except for C139S. These results suggest that tryptophan and cysteine residues conserved in ST3Gal I are critical for its activity...
  54. ncbi Regulation of natural killer cell-mediated swine endothelial cell lysis through genetic remodeling of a glycoantigen
    S Miyagawa
    Division of Organ Transplantation, Biomedical Research Center, Osaka University Graduate School of Medicine, Osaka University Medical School, Suita, Osaka, 565 0871, Japan ac jp
    J Biochem 126:1067-73. 1999
    ..Thus, the genetic remodeling of the alpha-Gal epitope and probably other glycoantigens as well can be expected to represent a new approach for overcoming not only indirect but also direct immunity to xenografts...
  55. doi A functional analysis of N-glycosylation-related genes on sialylation of recombinant erythropoietin in six commonly used mammalian cell lines
    Peiqing Zhang
    Bioprocessing Technology Institute, Agency for Science, Technology and Research A STAR, 20 Biopolis Way, 06 01 Centros 138668, Singapore
    Metab Eng 12:526-36. 2010
    ..Overexpression of ST6GalI, ST3GalIII or ST3GalIV, but not ST3GalVI, was able to improve EPO sialylation in these four cell lines...
  56. ncbi O-glycosylation regulates LNCaP prostate cancer cell susceptibility to apoptosis induced by galectin-1
    Hector F Valenzuela
    Department of Pathology, School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1732, USA
    Cancer Res 67:6155-62. 2007
    ..Resistance to galectin-1-induced apoptosis may directly contribute to the survival of prostate cancer cells as well as promote immune evasion by the tumor...
  57. ncbi Developmental patterns of GalBeta1,3(4)GlcNAc alpha2,3-sialyltransferase (ST3Gal III) expression in the mouse: in situ hybridization using DIG-labeled RNA probes
    M Y Ji
    Division of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Korea
    Arch Pharm Res 22:243-8. 1999
    ..These results indicate that ST3GAI III is differently expressed at developmental stages of mice embryo, and this may be importantly related with regulation of organogenesis in mice...
  58. doi Differences in CD75s- and iso-CD75s-ganglioside content and altered mRNA expression of sialyltransferases ST6GAL1 and ST3GAL6 in human hepatocellular carcinomas and nontumoral liver tissues
    Jamal Souady
    Institute of Medical Physics and Biophysics, University of Munster, Germany
    Glycobiology 21:584-94. 2011
    ..Thus, further experiments are required to explore the rationale behind the differential ganglioside level and to validate the applicability of CD75s- and iso-CD75s-gangliosides as targets for individual HCC therapies...
  59. doi Accelerated biosynthesis of neolacto-series glycosphingolipids in differentiated mouse embryonal carcinoma F9 cells detected by using dodecyl N-acetylglucosaminide as a saccharide primer
    Nao Ogasawara
    Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya Ku, Tokyo, Japan
    J Biochem 149:321-30. 2011
    ..Furthermore, the sialyltransferase activity of ST3GalVI sialylating paragloboside was enhanced with the increase in St3gal6 expression...
  60. ncbi Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver
    J Shang
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Academia Sinica, Beijing, China
    Eur J Biochem 265:580-8. 1999
    ..coli and both 32-kDa and 41-kDa proteins in P. pastoris. These results suggested that correct glycosylation of the enzyme might play a key role in its folding that may be directly related to the enzymatic activity...
  61. ncbi Comparison of genomic structures of four members of beta-galactoside alpha2,3-sialyltransferase genes in the mouse
    S Takashima
    Molecular Glycobiology, Frontier Research Program, The Institute of Physical and Chemical Research RIKEN, Wako, Saitama, Japan
    Cytogenet Cell Genet 89:101-6. 2000
    ..These results suggest that the gene pair Siat4 and Siat5 arose from a common ancestral gene, as did the two genes Siat3 and Siat4c...
  62. ncbi Genetic engineering of CHO cells producing human interferon-gamma by transfection of sialyltransferases
    K Fukuta
    Life Science Laboratory, Mitsui Chemicals, Inc, 1144 Togo, Mobara, Chiba 297 0017, Japan
    Glycoconj J 17:895-904. 2000
    ..9%). These results indicated that genetic engineering of STs is highly effective for regulating the terminal structures of sugar chains on recombinant proteins in CHO cells...
  63. ncbi Decrease in cell surface sialic acid in etoposide-treated Jurkat cells and the role of cell surface sialidase
    Y Azuma
    Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan
    Glycoconj J 17:301-6. 2000
    ..These results suggested that activation or exposure of sialidase on the cell surface was induced by etoposide treatment and was the main cause of the decrease in sialic acids...
  64. ncbi Gene structure and transcriptional regulation of human Gal beta1,4(3) GlcNAc alpha2,3-sialyltransferase VI (hST3Gal VI) gene in prostate cancer cell line
    A Taniguchi
    Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, 2 2 1, Miyama, Funabashi, Chiba, 274 8510, Japan
    Biochem Biophys Res Commun 287:1148-56. 2001
    ..The results suggested that the hST3Gal VI gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of transcription...
  65. ncbi Delineation of the minimal catalytic domain of human Galbeta1-3GalNAc alpha2,3-sialyltransferase (hST3Gal I)
    V Vallejo-Ruiz
    Unite de Glycobiologie Structurale et Fonctionnelle, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Biochim Biophys Acta 1549:161-73. 2001
    ..In addition, the present study demonstrates that the recombinant hST3Gal I polypeptides transiently expressed in COS-7 cells are glycosylated with complex and high mannose type glycans on each of the five potential N-glycosylation sites...
  66. ncbi The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin
    Bruce Walcheck
    Department of Veterinary Pathobiology, University Minnesota Academic Health Center, University of Minnesota, St Paul 55108, USA
    Blood 99:4063-9. 2002
    ..These findings support evidence that differential C2GnT activity results in T-cell subsets that express ligands for E-selectin, P-selectin, or both...
  67. ncbi CpsK of Streptococcus agalactiae exhibits alpha2,3-sialyltransferase activity in Haemophilus ducreyi
    Donald O Chaffin
    Department of Pediatrics, Division of Infectious Diseases, Children s Hospital and Regional Medical Center University of Washington, Seattle, 98105, USA
    Mol Microbiol 45:109-22. 2002
    ....
  68. ncbi Beta-galactoside alpha2,3-sialyltransferase-I gene expression during Th2 but not Th1 differentiation: implications for core2-glycan formation on cell surface proteins
    Nir Grabie
    Immunology Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Room 521, Boston, MA 02115, USA
    Eur J Immunol 32:2766-72. 2002
    ..We suggest that lack of ST3Gal-I expression in Th1 cells allows the formation of surface core2 O-glycans and supports their interactions with endothelial selectins...
  69. ncbi Production of cytidine 5'-monophosphate N-acetylneuraminic acid using recombinant Escherichia coli as a biocatalyst
    Sun Gu Lee
    Institute of Molecular Biology and Genetics, Seoul National University, Korea
    Biotechnol Bioeng 80:516-24. 2002
    ..coli extract and alpha2,3-sialyltransferase...
  70. ncbi Identification of nine alternatively spliced alpha2,3-sialyltransferase, ST3Gal IV, transcripts and analysis of their expression by RT-PCR and laser-induced fluorescent capillary electrophoresis (LIF-CE) in twenty-one human tissues
    A Grahn
    Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, SE 413 45 Goteborg, Sweden
    Glycoconj J 18:759-67. 2001
    ..We found a tissue specific expression of ST3Gal IV A1, A1 - 12, A1 + 18, A2, A2 - 12, A2 + 18 and B + 18 as well as a general expression of ST3Gal IV B and B - 12 isotranscripts in all tissues examined...
  71. ncbi Transcriptional regulation of human Galbeta1,3GalNAc/Galbeta1, 4GlcNAc alpha2,3-sialyltransferase (hST3Gal IV) gene in testis and ovary cell lines
    Akiyoshi Taniguchi
    Bionic Materials Group, Biomaterials Center, National Institute for Materials Science, 1 1 Namiki, Tsukuba, Ibaraki, Japan
    Biochem Biophys Res Commun 301:764-8. 2003
    ....
  72. pmc Enhanced insulin sensitivity in mice lacking ganglioside GM3
    Tadashi Yamashita
    Genetics of Development and Disease Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, 10 Center Drive, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 100:3445-9. 2003
    ..Importantly, the mutant mice were protected from high-fat diet-induced insulin resistance. Our results show that GM3 ganglioside is a negative regulator of insulin signaling, making it a potential therapeutic target in type 2 diabetes...
  73. ncbi Characterization of the promoter region of the human Galbeta1,3(4)GlcNAc alpha2,3-sialyltransferase III (hST3Gal III) gene
    Akiyoshi Taniguchi
    Biomaterials Center, National Institute for Materials Science, 1 1 Namiki, Ibaraki 305 0044, Tsukuba, Japan
    Biochim Biophys Acta 1626:92-6. 2003
    ..These results suggest that ubiquitous factors, such as Sp1, may be important for hST3Gal III gene expression...
  74. ncbi Human alpha2,3-sialyltransferase (ST3Gal II) is a stage-specific embryonic antigen-4 synthase
    Seiichi Saito
    Department of Urology, Tohoku University Graduate School of Medicine, Sendai 980 8574, Japan
    J Biol Chem 278:26474-9. 2003
    ..This study indicates that ST3Gal II is a MSGb5 (stage-specific embryonic antigen-4) synthase and that its increased expression level is closely related to renal carcinogenesis...
  75. ncbi Cloning and sequencing of nineteen transcript isoforms of the human alpha2,3-sialyltransferase gene, ST3Gal III; its genomic organisation and expression in human tissues
    Ammi Grahn
    Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, SE 413 45 Goteborg, Sweden
    Glycoconj J 19:197-210. 2002
    ..Neural and muscular tissues showed the most complex patterns and were distinctly different from all other tissues examined...
  76. ncbi Developmental patterns of mST3GalV mRNA expression in the mouse: in situ hybridization using DIG-labeled RNA probes
    M Y Ji
    Division of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk, Korea
    Arch Pharm Res 23:525-30. 2000
    ..These data indicate that mST3GalV is differently expressed at developmental stages of embryo, and this may be importantly related with regulation of organogenesis in mice...
  77. ncbi Photobacterium sp. JT-ISH-224 produces two sialyltransferases, alpha-/beta-galactoside alpha2,3-sialyltransferase and beta-galactoside alpha2,6-sialyltransferase
    Hiroshi Tsukamoto
    Glycotechnology Business Unit, Japan Tobacco Inc, Higashibara, Iwata, Shizuoka, Japan
    J Biochem 143:187-97. 2008
    ....
  78. ncbi Effect of N-acetylglucosaminyltransferase V on the expressions of other glycosyltransferases
    Peng Guo
    Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry, Shanghai Medical College, Fudan University, Shanghai 200032, PR China
    FEBS Lett 562:93-8. 2004
    ..However, it did not cause the increase of SLe(x) synthesis. Transfection of antisense GnTV into H7721 cells showed entirely opposite effects on the expression of above-mentioned SLe(x) and glycosyltransferases as the sense GnTV...
  79. ncbi Transfection of 2,6 and 2,3-sialyltransferase genes and GlcNAc-transferase genes into human glioma cell line U-373 MG affects glycoconjugate expression and enhances cell death
    G Dawson
    Department of Pediatrics MC 4068, University of Chicago School of Medicine, 5841 S Maryland Avenue, Chicago, IL 60637, USA
    J Neurochem 89:1436-44. 2004
    ..Thus altered surface protein glycosylation of a human glioblastoma cell line can lead to lowered resistance to chemotherapeutic agents...
  80. ncbi Identification of seven new alpha2,3-sialyltransferase III, ST3Gal III, transcripts from human foetal brain
    Ammi Grahn
    Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, SE 413 45 Goteborg, Sweden
    Glycoconj J 20:493-500. 2004
    ..Isotranscripts ST3Gal III C9 and B10 were distinctly different from all other forms identified so far. The splice variants reported here are unlikely to express enzymatic activities but may other biological functions...
  81. pmc Reversion of the Jun-induced oncogenic phenotype by enhanced synthesis of sialosyllactosylceramide (GM3 ganglioside)
    Yutaka Miura
    Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122 4302, USA
    Proc Natl Acad Sci U S A 101:16204-9. 2004
    ..The amounts of this complex are greatly reduced in transformed cells. Expression of GM3 and consequent reversion of the transformed phenotype results in increased levels of that microdomain complex...
  82. ncbi Cloning and expression of cDNA for a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase from the CEM T-cell line
    V Giordanengo
    Laboratoire de Virologie, Faculte de Medecine, Nice, France
    Eur J Biochem 247:558-66. 1997
    ....
  83. ncbi Altered mRNA expressions of sialyltransferases in ovarian cancers
    Peng Hui Wang
    Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, and Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
    Gynecol Oncol 99:631-9. 2005
    ....
  84. ncbi Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms
    M Gawlitzek
    Process Sciences, Genentech, Inc, South San Francisco, California 94080, USA
    Biotechnol Bioeng 68:637-46. 2000
    ..Consequently, ammonium seems to alter the carbohydrate biosynthesis of TNFR-IgG by a pH-mediated effect on glycosyltransferase activity...
  85. ncbi Alpha 2,3-sialyltransferase-IV is essential for L-selectin ligand function in inflammation
    Markus Sperandio
    Children s Hospital, University of Heidelberg, Heidelberg, Germany
    Eur J Immunol 36:3207-15. 2006
    ....
  86. ncbi Lactosylceramide alpha2,3-sialyltransferase is induced via a PKC/ERK/CREB-dependent pathway in K562 human leukemia cells
    Hee Jung Choi
    Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, Sungkyunkwan University, Suwon 440 746, Korea
    Mol Cells 23:138-44. 2007
    ..Our results demonstrate that induction of GM3 synthase during megakaryocytic differentiation in PMA-stimulated human leukemia K562 cells depends upon the PKC/ERK/CREB pathway...
  87. ncbi A remodeling system of the 3'-sulfo-Lewis a and 3'-sulfo-Lewis x epitopes
    N Ikeda
    Department of Biochemistry and Department of Surgery, Osaka University Medical School, Suita, Osaka 565 0871, Japan
    J Biol Chem 276:38588-94. 2001
    ..The present remodeling system might be of potential use as a tool for the study of the physiological roles of 3'-sulfated Lewis epitopes, including interaction with selectins...
  88. pmc The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase
    Yanhong Li
    Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA
    Biochem Biophys Res Commun 361:555-60. 2007
    ..Here, we show that Hd0053 of H. ducreyi genomic strain 35000HP, the third member of the glycosyltransferase family 80 (GT80), also encodes an alpha2,3-sialyltransferase that may be important for LOS sialylation...
  89. ncbi N-glycan structures and associated gene expression reflect the characteristic N-glycosylation pattern of human hematopoietic stem and progenitor cells
    Heidi Hemmoranta
    Finnish Red Cross Blood Service, Research and Development, Helsinki, Finland
    Exp Hematol 35:1279-92. 2007
    ..Cell surface glycans contribute to the adhesion capacity of cells and are essential in cellular signal transduction. Yet, the glycosylation of hematopoietic stem and progenitor cells (HSPC), such as CD133+ cells, is poorly explored...
  90. ncbi Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16
    Yoshimitsu Takakura
    Glycotechnology Business Unit, Japan Tobacco Inc, 700 Higashibara, Iwata, Shizuoka, Japan
    J Biochem 142:403-12. 2007
    ..These results suggest that the enzyme can be used as a powerful tool for the study for glycotechnology...
  91. ncbi IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewisx epitopes in the human bronchial mucosa
    Sophie Groux-Degroote
    Unite de Glycobiologie Structurale et Fonctionnelle, UMR CNRS no 8576, Batiment C9, Universite des Sciences et Technologies de Lille, F 59655 Villeneuve d Ascq, Lille, France
    Biochem J 410:213-23. 2008
    ..In conclusion, our results indicate that IL-6 and -8 may contribute to the increased levels of sialyl-Lewis(x) and 6-sulfo-sialyl-Lewis(x) epitopes on human airway mucins from patients with CF...
  92. ncbi The evolution of galactose alpha2,3-sialyltransferase: Ciona intestinalis ST3GAL I/II and Takifugu rubripes ST3GAL II sialylate Galbeta1,3GalNAc structures on glycoproteins but not glycolipids
    Friederike Lehmann
    Institute for Glycomics, Gold Coast Campus, Griffith University, QLD, 4222, Australia
    Glycoconj J 25:323-34. 2008
    ..This suggests that during the evolution of the ST3Gal II, probably following the separation of the teleosts, a significant shift in substrate specificity enabling the sialylation of gangliosides took place...

Research Grants2

  1. Distinction of skin homing T cells that bind P-selectin
    Bruce Walcheck; Fiscal Year: 2004
    ..abstract_text> ..
  2. Regulation of inflammation: sheddases and CD62L
    Bruce Walcheck; Fiscal Year: 2009
    ..This knowledge will be highly useful in the development of more specific therapies tailored to inflammatory processes and immunity. ..