alpha tocopherol transfer protein

Summary

Gene Symbol: alpha tocopherol transfer protein
Description: alpha tocopherol transfer protein
Alias: ATTP, AVED, TTP1, alphaTTP, alpha-tocopherol transfer protein, alpha-TTP, tocopherol (alpha) transfer protein (ataxia (Friedreich-like) with vitamin E deficiency)
Species: human

Top Publications

  1. ncbi Mycobacteriophage D29 contains an integration system similar to that of the temperate mycobacteriophage L5
    G Ribeiro
    Laboratory of Molecular Virology, Gulbenkian Institute of Science, Oeiras, Portugal
    Microbiology 143:2701-8. 1997
  2. pmc Increased atherosclerosis in hyperlipidemic mice deficient in alpha -tocopherol transfer protein and vitamin E
    Y Terasawa
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 97:13830-4. 2000
  3. pmc Delayed-onset ataxia in mice lacking alpha -tocopherol transfer protein: model for neuronal degeneration caused by chronic oxidative stress
    T Yokota
    Department of Neurology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 98:15185-90. 2001
  4. ncbi Molecular genetic and expression analysis of alpha-tocopherol transfer protein mRNA in German shepherd dogs with degenerative myelopathy
    Henry Fechner
    Department of Cardiology and Pneumology, University Hospital Benjamin Franklin, Freie Universitat, Berlin, Germany
    Berl Munch Tierarztl Wochenschr 116:31-6. 2003
  5. ncbi pH-dependent translocation of alpha-tocopherol transfer protein (alpha-TTP) between hepatic cytosol and late endosomes
    Masakuni Horiguchi
    Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
    Genes Cells 8:789-800. 2003
  6. doi Epidemiological, clinical, paraclinical and molecular study of a cohort of 102 patients affected with autosomal recessive progressive cerebellar ataxia from Alsace, Eastern France: implications for clinical management
    M Anheim
    Departement de Neurologie, Hopital Civil, Centre Hospitalier Universitaire de Strasbourg, 1, Place de l Hopital, 67000, Strasbourg, France
    Neurogenetics 11:1-12. 2010
  7. ncbi Vitamin E: action, metabolism and perspectives
    E Herrera
    Facultad de Ciencias Experimentales y Técnicas, Universidad San Pablo CEU, Madrid, Spain
    J Physiol Biochem 57:43-56. 2001
  8. ncbi Genetic analysis of early onset cerebellar ataxia with retained tendon reflexes in four Tunisian families
    N Marzouki
    Labratoire de Génétique Moléculaire, Université Mohamed Ben Abdellah, Faculte des Sciences, Fes, Morocco
    Clin Genet 59:257-62. 2001
  9. ncbi Vitamin E trafficking
    Maret G Traber
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331 6512, USA
    Ann N Y Acad Sci 1031:1-12. 2004
  10. pmc Depletion of vitamin E increases amyloid beta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease
    Yoichiro Nishida
    Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519 Japan
    J Biol Chem 284:33400-8. 2009

Research Grants

  1. Carrie Finno; Fiscal Year: 2016
  2. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2012
  3. Integration and Excision by Serine Intergrases
    Graham F Hatfull; Fiscal Year: 2013
  4. Inna Djagaeva; Fiscal Year: 2014
  5. Recombination-mediated cassette exchange in Aedes aegypti mosquitoes
    Zach N Adelman; Fiscal Year: 2013
  6. Hugo J Bellen; Fiscal Year: 2016
  7. ATTACHMENT SITE RECOGNITION IN PHAGE LAMBDA INTEGRATION
    MICHAEL BRUIST; Fiscal Year: 1991
  8. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2009
  9. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2005
  10. Oxidant induced gene expression in vitamin E deficient *
    Kishorchandra Gohil; Fiscal Year: 2002

Detail Information

Publications207 found, 100 shown here

  1. ncbi Mycobacteriophage D29 contains an integration system similar to that of the temperate mycobacteriophage L5
    G Ribeiro
    Laboratory of Molecular Virology, Gulbenkian Institute of Science, Oeiras, Portugal
    Microbiology 143:2701-8. 1997
    ..excisase gene and the integrase gene had a lower than average G+C content and showed the presence of the same attP core sequence as mycobacteriophage L5...
  2. pmc Increased atherosclerosis in hyperlipidemic mice deficient in alpha -tocopherol transfer protein and vitamin E
    Y Terasawa
    Gladstone Institute of Cardiovascular Disease, P O Box 419100, San Francisco, CA 94141 9100, USA
    Proc Natl Acad Sci U S A 97:13830-4. 2000
    ..Ttpa(-/-) mice are a genetic model of vitamin E deficiency and should be valuable for studying other diseases in which oxidative stress is thought to play a role...
  3. pmc Delayed-onset ataxia in mice lacking alpha -tocopherol transfer protein: model for neuronal degeneration caused by chronic oxidative stress
    T Yokota
    Department of Neurology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 98:15185-90. 2001
    ..Mutations of the alpha-TTP gene are linked to ataxia with isolated vitamin E deficiency (AVED)...
  4. ncbi Molecular genetic and expression analysis of alpha-tocopherol transfer protein mRNA in German shepherd dogs with degenerative myelopathy
    Henry Fechner
    Department of Cardiology and Pneumology, University Hospital Benjamin Franklin, Freie Universitat, Berlin, Germany
    Berl Munch Tierarztl Wochenschr 116:31-6. 2003
    ..we analyzed the expression and the nucleotide sequence of the canine alpha-tocopherol transfer protein (alpha Ttp) of German shepherd dogs with DM in order to determine whether a deficiency or a defect of the alpha Ttp could ..
  5. ncbi pH-dependent translocation of alpha-tocopherol transfer protein (alpha-TTP) between hepatic cytosol and late endosomes
    Masakuni Horiguchi
    Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan
    Genes Cells 8:789-800. 2003
    ..To explore the mechanism of alpha-TTP mediated alpha-tocopherol secretion, we investigated drugs which may affect this secretion...
  6. doi Epidemiological, clinical, paraclinical and molecular study of a cohort of 102 patients affected with autosomal recessive progressive cerebellar ataxia from Alsace, Eastern France: implications for clinical management
    M Anheim
    Departement de Neurologie, Hopital Civil, Centre Hospitalier Universitaire de Strasbourg, 1, Place de l Hopital, 67000, Strasbourg, France
    Neurogenetics 11:1-12. 2010
    ..with autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), one with ataxia with vitamin E deficiency (AVED) and one with autosomal recessive cerebellar ataxia type 2 (ARCA2)...
  7. ncbi Vitamin E: action, metabolism and perspectives
    E Herrera
    Facultad de Ciencias Experimentales y Técnicas, Universidad San Pablo CEU, Madrid, Spain
    J Physiol Biochem 57:43-56. 2001
    ..The most intriguing is its interaction with other antioxidants that may explain how foods containing small amounts of vitamin E provide greater benefits than larger doses of vitamin E alone...
  8. ncbi Genetic analysis of early onset cerebellar ataxia with retained tendon reflexes in four Tunisian families
    N Marzouki
    Labratoire de Génétique Moléculaire, Université Mohamed Ben Abdellah, Faculte des Sciences, Fes, Morocco
    Clin Genet 59:257-62. 2001
    ..that three of the four families studied were not linked to Friedreich's ataxia (FA), vitamin E deficiency ataxia (AVED), and autosomal dominant cerebellar ataxia (ADCA) loci...
  9. ncbi Vitamin E trafficking
    Maret G Traber
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331 6512, USA
    Ann N Y Acad Sci 1031:1-12. 2004
    ..Clearly, critical information is lacking in our understanding of the mechanism by which alpha-TTP facilitates the preferential enrichment of VLDLs with alpha-tocopherol...
  10. pmc Depletion of vitamin E increases amyloid beta accumulation by decreasing its clearances from brain and blood in a mouse model of Alzheimer disease
    Yoichiro Nishida
    Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519 Japan
    J Biol Chem 284:33400-8. 2009
    ..These results suggest that lipid peroxidation due to depletion of alpha-tocopherol impairs Abeta clearances from the brain and from the blood, possibly causing increased Abeta accumulation in Ttpa(-/-)APPsw mouse brain and plasma...
  11. ncbi Molecular mechanisms of vitamin E transport
    Achim Stocker
    Institute of Microbiology, Swiss Federal Institute of Technology Zurich, Schmelzbergstr 7, 8092 Zurich, Switzerland
    Ann N Y Acad Sci 1031:44-59. 2004
    ....
  12. doi ATP-binding cassette transporter A1 is involved in hepatic alpha-tocopherol secretion
    Mototada Shichiri
    Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    J Nutr Biochem 21:451-6. 2010
    ..In a mouse in vivo experiment, addition of 1% probucol to the diet decreased plasma alpha-tocopherol concentrations. These results strongly suggest that ABCA1 is substantially involved in hepatic alpha-tocopherol secretion...
  13. pmc Integration into the phage attachment site, attB, impairs multicellular differentiation in Stigmatella aurantiaca
    Susanne Muller
    Georgia Institute of Technology, School of Biology, 310 Ferst Drive, Atlanta, Georgia 30332 0230, USA
    J Bacteriol 188:1701-9. 2006
    ..However, plasmids containing Mx8 fragments encoding the integrase and attP are able to integrate at the attB locus in the S. aurantiaca genome by site-specific recombination...
  14. pmc Genomic characterization of the filamentous integrative bacteriophages {phi}RSS1 and {phi}RSM1, which infect Ralstonia solanacearum
    Takeru Kawasaki
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1 3 1 Kagamiyama, Higashi Hiroshima 739 8530, Japan
    J Bacteriol 189:5792-802. 2007
    ..Interestingly, ORF14, located next to the attP site on the phiRSM1 genome, showed high amino acid sequence homology with bacterial DNA recombinases and resolvases,..
  15. ncbi [Negative cortisone attitude in patients with bronchial asthma]
    W Woller
    Klinisches Institut und Klinik für Psychosomatische Medizin und Psychotherapie, Heinrich Heine Universitat Dusseldorf
    Pneumologie 46:326-9. 1992
    ..with bronchial asthma before and after participation in the Düsseldorf Asthma treatment and teaching programme (ATTP) and one year later...
  16. ncbi Gene expression profile of oxidant stress and neurodegeneration in transgenic mice deficient in alpha-tocopherol transfer protein
    Kishorchandra Gohil
    Center for Comparative Respiratory and Medicine, Department of Internal Medicine, University of California, Davis, CA 95616, USA
    Free Radic Biol Med 35:1343-54. 2003
    ..The repression of specific genes that determine synaptic plasticity and neuronal development may account for suppressed electrophysiological activities of cortex and impaired behavior in Ttpa(-/-) mice...
  17. doi A novel delins mutation in the alpha-TTP gene in a family segregating ataxia with isolated vitamin E deficiency
    Miguel Fernandez-Burriel
    Research Unit Clinical Chemistry Laboratory, Hospital de Mérida, Merida, Badajoz, Spain
    Pediatr Res 64:262-4. 2008
    ..These findings suggest that other genes may play a role in producing this atypical biochemical profile...
  18. pmc Mice lacking alpha-tocopherol transfer protein gene have severe alpha-tocopherol deficiency in multiple regions of the central nervous system
    Kishorchandra Gohil
    Department of Internal Medicine, Genome and Biomedical Sciences Facility, University of California, Davis, CA 95616, USA
    Brain Res 1201:167-76. 2008
    ..Interestingly, functional abnormality (ataxia) develops only very late in alpha-TTP-KO mice in spite of the severe alpha-tocopherol deficiency in the brain starting at an early age...
  19. doi SXT-related integrating conjugative element and IncC plasmids in Vibrio cholerae O1 strains in Eastern Africa
    N Pugliese
    Dipartimento di Genetica e Microbiologia, Universita di Bari, Bari, Italy
    J Antimicrob Chemother 63:438-42. 2009
    ..The objective of this study was to investigate the extent of resistance patterns and associated mobile genetic elements in epidemic V. cholerae O1 El Tor strains isolated from Eastern Africa in the late 1990s...
  20. ncbi Sequence analysis of a unique temperature phage: mycoplasma virus L2
    J Maniloff
    Department of Microbiology and Immunology, University of Rochester, NY 14642
    Gene 141:1-8. 1994
    ..The putative attP integration site has been mapped to an intergenic region, 280-bp downstream from ORF 5...
  21. ncbi Acceleration of age-related changes in the retina in alpha-tocopherol transfer protein null mice fed a Vitamin E-deficient diet
    Masaki Tanito
    Department of Ophthalmology, Shimane University School of Medicine, Shimane, Japan
    Invest Ophthalmol Vis Sci 48:396-404. 2007
    ..To assess the role of vitamin E (VE) in age-related changes in the retinal tissues by using a mouse model of severe VE deficiency...
  22. ncbi Tocopherol transfer protein deficiency modifies nuclear receptor transcriptional networks in lungs: modulation by cigarette smoke in vivo
    K Gohil
    Pulmonary and Critical Care Medicine, Genome and Biomedical Sciences Facility, 451 East Health Sciences Drive, University of California, Davis, CA 95616, USA
    Mol Aspects Med 28:453-80. 2007
    ..this study we have used mice with severe AT deficiency inflicted at birth by the deletion of AT transfer protein (ATTP) which is abundantly expressed in the liver and regulates systemic concentrations of AT...
  23. ncbi Clinical comparison between AVED patients with 744 del A mutation and Friedreich ataxia with GAA expansion in 15 Moroccan families
    Ali Benomar
    Laboratoire de Neurogénétique, Service de Neurologie, Hopital des specialites, BP 6220 Rabat Instituts, Rabat, Morocco
    J Neurol Sci 198:25-9. 2002
    ..in the alpha-tocopherol transfer protein (alpha-TTP) gene, characteristic of ataxia with vitamin E deficiency (AVED). The other eight families (16 patients) had GAA expansions in the first intron of the frataxin gene...
  24. ncbi Selective accumulation of alpha-tocopherol in Drosophila is associated with cytochrome P450 tocopherol-omega-hydroxylase activity but not alpha-tocopherol transfer protein
    Robert S Parker
    Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA
    Biochem Biophys Res Commun 338:1537-41. 2005
    ..The selective pressure favoring this trait and its remarkable conservation from insects to humans requires elucidation...
  25. ncbi Galactosamine-induced acute liver injury in rats reduces hepatic alpha-tocopherol transfer protein production
    Asako Takenaka
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University, Yamagata, Japan
    J Nutr Sci Vitaminol (Tokyo) 53:366-71. 2007
    ..Serum alpha-tocopherol concentration was not directly affected by the acute change in hepatic alpha-TTP level, suggesting that the chronic changes in alpha-TTP activity would be necessary to regulate serum alpha-tocopherol concentration...
  26. ncbi Control of prophage integration and excision in bacteriophage P2: nucleotide sequences of the int gene and att sites
    A Yu
    Department of Microbial Genetics, Karolinska Institutet, Stockholm, Sweden
    Gene 80:1-11. 1989
    ..P2 into the Escherichia coli host genome involves recombination between two specific attachment sites, attP and attB, one on the phage and the other on the host genome, respectively...
  27. ncbi A method to maintain introduced DNA sequences stably and safely on the bacterial chromosome: application of prophage integration and subsequent designed excision
    M Shimizu-Kadota
    Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186 8650, Japan
    J Biotechnol 89:73-9. 2001
    ..gene as a selection marker, N- and C-terminally-truncated Cm-resistance genes were inserted into both sides of the attP of phi FSW, within which the site-specific recombination took place with the attB of phi FSW on the recipient ..
  28. ncbi Dietary zinc restriction in rats alters antioxidant status and increases plasma F2 isoprostanes
    Richard S Bruno
    Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA
    J Nutr Biochem 18:509-18. 2007
    ..Collectively, zinc deficiency increased oxidative stress, which may be partially explained by increased CYP activity and reductions in hepatic alpha-tocopherol and gamma-tocopherol and in plasma uric acid...
  29. pmc Isolation of new Stenotrophomonas bacteriophages and genomic characterization of temperate phage S1
    Pilar Garcia
    Area de Microbiologia, Facultad de Medicina, Universidad de Oviedo, Spain
    Appl Environ Microbiol 74:7552-60. 2008
    ..The attP site of S1 may be located within a gene that presents over 75% homology to a Stenotrophomonas chromosomal ..
  30. pmc Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14
    Carlos A Santiviago
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile, P O Box 174, Correo 22, Santiago, Chile
    J Bacteriol 192:2246-54. 2010
    ..The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed...
  31. pmc Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555 Japan
    Malar J 9:101. 2010
    ..However, mechanisms of this Plasmodium inhibition, in addition to means by which to exploit this finding as a therapeutic strategy, remain unclear...
  32. ncbi Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces
    F Boccard
    Laboratoire de Biologie et Génétique Moléculaire, URA CNRS D 0086, Orsay, France
    Plasmid 21:59-70. 1989
    ..A 58-bp sequence (att) present in both pSAM2 (attP) and S...
  33. ncbi Tocopherol-associated protein is a ligand-dependent transcriptional activator
    J Yamauchi
    Division of Applied Food Research, National Institute of Health and Nutrition, 1 23 1 Toyama, Shinjyuku, Japan
    Biochem Biophys Res Commun 285:295-9. 2001
    ..These results suggest that the biological function of alpha-tocopherol is not only as an antioxidant but also as a transcriptional regulator of gene expression via association with a transcription factor TAP...
  34. pmc Study of plastid genome stability in tobacco reveals that the loss of marker genes is more likely by gene conversion than by recombination between 34-bp loxP repeats
    Tarinee Tungsuchat-Huang
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Plant Physiol 153:252-9. 2010
    ..The P1 phage loxP sites are identical 34-bp direct repeats, whereas the phiC31 phage attB/attP sites are 54- and 215-bp sequences with partial homology within the 54-bp region...
  35. ncbi Protective effect of vitamin E supplements on experimental atherosclerosis is modest and depends on preexisting vitamin E deficiency
    Cacang Suarna
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia
    Free Radic Biol Med 41:722-30. 2006
    ..These results show that vitamin E at best has a modest effect on experimental atherosclerosis in hyperlipidemic mice, and only in situations of severe vitamin E deficiency and independent of lipid oxidation in the vessel wall...
  36. ncbi Liver alpha-tocopherol transfer protein and its mRNA are differentially altered by dietary vitamin E deficiency and protein insufficiency in rats
    H M Shaw
    Laboratory of Nutritional Biochemistry, Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan 10716
    J Nutr 128:2348-54. 1998
    ..On the other hand, protein inadequacy appeared to down-regulate the expression of the alpha-TTP gene...
  37. ncbi Complete nucleotide sequence, molecular analysis and genome structure of bacteriophage A118 of Listeria monocytogenes: implications for phage evolution
    M J Loessner
    Institut fur Mikrobiologie, FML Weihenstephan, Technische Universitat Munchen, Weihenstephaner Berg 3, 85350 Freising, Germany
    Mol Microbiol 35:324-40. 2000
    ..We have also identified the A118 attachment site attP and the corresponding attB in Listeria monocytogenes, and show that site-specific integration of the A118 prophage ..
  38. ncbi Structure and function of alpha-tocopherol transfer protein: implications for vitamin E metabolism and AVED
    K Christopher Min
    Department of Neurology, Columbia University, New York, New York 10032, USA
    Vitam Horm 76:23-43. 2007
    ..in the protein are a cause of a progressive neurodegenerative disorder known as ataxia with vitamin E deficiency (AVED)...
  39. pmc Alpha-Tocopherol Transfer Protein (alpha-TTP): Insights from Alpha-Tocopherol Transfer Protein Knockout Mice
    Yunsook Lim
    Department of Food and Nutrition, Kyung Hee University, Seoul 130 701, Korea
    Nutr Res Pract 1:247-53. 2007
    ..Furthermore, alpha-TTP(-/-) mice appear to be useful to elucidate functions of alpha-TTP beyond its well recognized functions of transferring alpha-T from liver to plasma lipoprotein fractions...
  40. pmc Postmortem study of ataxia with retinitis pigmentosa by mutation of the alpha-tocopherol transfer protein gene
    T Yokota
    Department of Neurology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    J Neurol Neurosurg Psychiatry 68:521-5. 2000
    ..It is therefore considered that oral supplementation of vitamin E should effectively counteract the progression of ataxia...
  41. pmc Characterization of the integrase gene and attachment site for the Myxococcus xanthus bacteriophage Mx9
    Bryan Julien
    Kosan Biosciences, Inc, Hayward, California 94545, USA
    J Bacteriol 185:6325-30. 2003
    ..Mx9 integration requires a single protein, Int. Analysis of integration revealed that the phage attachment site (attP) is contained in the int gene and that upon integration, the 3' end of the int gene is altered...
  42. doi Conjugated linoleic acid causes a marked increase in liver alpha-tocopherol and liver alpha-tocopherol transfer protein in C57BL/6 J mice
    Pei Min Chao
    Institute of Nutrition and Department of Nutrition, China Medical University, Taichung, Taiwan
    Int J Vitam Nutr Res 80:65-73. 2010
    ..The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to alpha-tocopherol accumulation...
  43. ncbi Suicide substrates reveal properties of the homology-dependent steps during integrative recombination of bacteriophage lambda
    A B Burgin
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892 4034, USA
    Curr Biol 5:1312-21. 1995
    ....
  44. ncbi Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296
    M M Adams
    Division of Biochemistry and Molecular Biology, School of Life Sciences, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Microbiology 147:851-60. 2001
    ..DNAs homologous to bacteriophage int and attP were located upstream of gtrA(IV), suggesting that this region of the NCTC 8296 genome may have originated from a ..
  45. ncbi Ataxia with vitamin E deficiency and severe dystonia: report of a case
    Agathe Roubertie
    Service de Neuropediatrie, Hopital Saint Eloi, 80 avenue Augustin Fliche, 34295 Montpellier Cedex 5, France
    Brain Dev 25:442-5. 2003
    ..Dystonia has rarely been described in ataxia with isolated vitamin E deficiency (AVED) patients...
  46. ncbi Vitamin E deficiency ataxia with (744 del A) mutation on alpha-TTP gene: genetic and clinical peculiarities in Moroccan patients
    Naima Marzouki
    Molecular Genetics Laboratory, Faculty of Sciences, The Department of Neurology, University of Fez Sidi Med Benabdellah, Hospital of Rabat, Morocco
    Eur J Med Genet 48:21-8. 2005
    Ataxia with vitamin E deficiency (AVED) is an autosomal recessive disease characterized clinically by neurological symptoms with often striking resemblance to those of Friedreich's ataxia (FA)...
  47. pmc The Bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages
    Shanmuga Sozhamannan
    Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, Maryland 20852, USA
    BMC Microbiol 6:34. 2006
    ..The B. anthracis genome sequence contains four putative lambdoid prophages. We undertook this study in order to understand whether the four prophages are unique to B. anthracis and whether they produce active phages...
  48. pmc Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363
    M van de Guchte
    National Food Biotechnology Centre, University College, Cork, Ireland
    Appl Environ Microbiol 60:2324-9. 1994
    The DNA sequence of the int-attP region of the small-isometric-headed lactococcal bacteriophage Tuc2009 is presented...
  49. pmc Integration of bacteriophage Mx8 into the Myxococcus xanthus chromosome causes a structural alteration at the C-terminal region of the IntP protein
    N Tojo
    Department of Biology, Tokyo Metropolitan University, Japan
    J Bacteriol 178:4004-11. 1996
    ..Here, we characterize the mechanism of Mx8 integration into the M. xanthus chromosome. The Mx8 attachment site, attP, the M...
  50. ncbi Architectural flexibility in lambda site-specific recombination: three alternate conformations channel the attL site into three distinct pathways
    A M Segall
    Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892 4034, USA
    Genes Cells 1:453-63. 1996
    ..life cycle, the Integrase (Int) protein carries out recombination between two different sets of DNA substrates: attP and attB in integration, attL and attR in excision...
  51. ncbi A highly efficient and stable system for site-specific integration of genes and plasmids into the phage phiLC3 attachment site (attB) of the Lactococcus lactis chromosome
    D Lillehaug
    Department of Biotechnological Sciences, The Agricultural University of Norway, As
    Gene 188:129-36. 1997
    ..A 1.6-kb recombinogenic DNA cassette, containing the phiLC3 integrase gene (int) and the phage attachment site (attP), mediated site-specific integration of a single marker-gene, as well as of a replication-thermosensitive (-ts) ..
  52. pmc The transcriptional switch of bacteriophage WPhi, a P2-related but heteroimmune coliphage
    T Liu
    Department of Genetics, Stockholm University, S 106 91 Stockholm, Sweden
    J Virol 73:9816-26. 1999
    ..The DNA sequence of the whole early-control region and the int and attP region of phage WPhi has been determined. The phage integration site was located at 88...
  53. ncbi Genomic sequence and analysis of the atypical temperate bacteriophage N15
    V Ravin
    Center for Bioengineering, Russian Academy of Science, Moscow, Russia
    J Mol Biol 299:53-73. 2000
    ..prophage ends is close to the center of the genome in a similar location to that occupied by the attachment site, attP, in lambda and its relatives and defines the boundary between the left and right arms...
  54. pmc Ataxia caused by mutations in the alpha-tocopherol transfer protein gene
    F Usuki
    Department of Clinical Medicine, National Institute for Minamata Disease, 4058 18 Hama, Minamata 867 0008, Japan
    J Neurol Neurosurg Psychiatry 69:254-6. 2000
    ..Supplementary therapy increased her serum vitamin E concentration to the normal range with mild improvement of the deep senses...
  55. ncbi Instability and site-specific excision of integration-proficient mycobacteriophage L5 plasmids: development of stably maintained integrative vectors
    B Springer
    Institut fur Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany
    Int J Med Microbiol 290:669-75. 2001
    ..By providing attP in cis and integrase function in trans, vectors integrating at the attB site are stably maintained, even when ..
  56. ncbi Expression plasmid with a very tight two-step control: Int/att-mediated gene inversion with respect to the stationary promoter
    M Sektas
    University of Gdansk, Department of Microbiology, 80 822 Gdansk, ul Kladki 24, Poland
    Gene 267:213-20. 2001
    ..to transcribe genes cloned in the proximal multiple cloning site (MCS), which was flanked by convergent attB and attP sites. The gene of interest was cloned into MCS in the OFF orientation, i.e. facing the promoter(s)...
  57. ncbi Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages
    A J Clark
    Department of Molecular and Cell Biology, Division of Life Sciences, MS74 157, Berkeley, CA 94720, USA
    J Mol Biol 311:657-79. 2001
    ..Integration, on the other hand, resembles that of satellite phage P4 in that the attP sequence lies between the leftward promoter and int rather than downstream of int...
  58. ncbi Genomic characterization of human SEC14L1 splice variants within a 17q25 candidate tumor suppressor gene region and identification of an unrelated embedded expressed sequence tag
    L M Kalikin
    Department of Internal Medicine and Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Mamm Genome 12:925-9. 2001
    ..Coding exon-specific PCR primers were designed to aid in future expression and mutational analyses...
  59. ncbi NMR solution structure of ATTp, an Arabidopsis thaliana trypsin inhibitor
    Qin Zhao
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    Biochemistry 41:12284-96. 2002
    ..The overall fold of ATT(p) is distinct from those of other classes of serine proteinase inhibitors except in the inhibitor loop; therefore, it represents a new inhibitor fold...
  60. ncbi Phosphorylation of supernatant protein factor enhances its ability to stimulate microsomal squalene monooxygenase
    Dev K Singh
    Division of Pharmaceutical Sciences, College of Pharmacy and The Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536 0082, USA
    J Biol Chem 278:5646-51. 2003
    ..Supernatant protein factor thus appears to be regulated both post-translationally through phosphorylation and at the level of expression. Phosphorylation may provide a means for the rapid short term modulation of cholesterol synthesis...
  61. pmc The IntP C-terminal segment is not required for excision of bacteriophage Mx8 from the Myxococcus xanthus chromosome
    Nobuki Tojo
    Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji shi, Tokyo 192 0397, Japan
    J Bacteriol 185:2187-93. 2003
    ..We previously demonstrated that the Mx8 attP site is located within the coding sequence of the Mx8 intP gene. Hence, the integration of Mx8 into the M...
  62. ncbi Distribution and transfer pathways of antioxidant molecules inside the first trimester human gestational sac
    Eric Jauniaux
    Academic Department of Obstetrics and Gynaecology, Royal Free and University College London Medical School, London WC1E 6HX, UK
    J Clin Endocrinol Metab 89:1452-8. 2004
    ..This finding indicates that the uterine glands and the secondary yolk sac play key roles in supplying this essential vitamin to the developing fetus before the placental circulations are established...
  63. ncbi In silico search for single nucleotide polymorphisms in genes important in vitamin E homeostasis
    Frank Döring
    Research Group Molecular Nutrition, University of Kiel, Hermann Weigmann Street 1, 24103 Kiel, Germany
    IUBMB Life 56:615-20. 2004
    ..We suggest that future studies are aimed at addressing the role of such SNPs in vitamin E homeostasis...
  64. pmc Complete and persistent phenotypic correction of phenylketonuria in mice by site-specific genome integration of murine phenylalanine hydroxylase cDNA
    Li Chen
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, One Gustave L Levy Place, Box 1496, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 102:15581-6. 2005
    ..we showed that this phage integrase functions efficiently in mouse cells, and several naturally occurring pseudo-attP sites located in the intergenic regions of the mouse genome have been identified and molecularly characterized...
  65. ncbi Preparation of fluorescent tocopherols for use in protein binding and localization with the alpha-tocopherol transfer protein
    Phillip Nava
    Department of Chemistry and Centre for Biotechnology, Brock University, St Catharines, Ont, Canada L2S 3A1
    Bioorg Med Chem 14:3721-36. 2006
    ..Thus, compounds have been prepared that allow the investigation of the rate of alpha-TTP-mediated inter-membrane transfer of alpha-tocopherol and to investigate the mechanism of alpha-TTP function at membranes of different composition...
  66. ncbi Genetic vitamin E deficiency does not affect MPTP susceptibility in the mouse brain
    Yong Ri Ren
    Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan
    J Neurochem 98:1810-6. 2006
    ..Our results suggest that vitamin E does not play a major protective role in MPTP-induced nigrostriatal dopaminergic neurodegeneration in the brain...
  67. ncbi Rationale and delineation of a composite index of relative antitumoural efficacy (In-RATE)
    Jean Yves Blay
    Hopital Edouard Herriot, Oncologie Médicale, Pavillon E, 5 Place d Arsonval, 69437 Lyon Cedex 03, France
    Crit Rev Oncol Hematol 64:106-14. 2007
    ....
  68. doi Evidence for the horizontal transfer of an integrase gene from a fusellovirus to a pRN-like plasmid within a single strain of Sulfolobus and the implications for plasmid survival
    Xu Peng
    Danish Archaea Centre, Department of Molecular Biology, Biocenter, Copenhagen University, Ole Maaløes Vej 5, Copenhagen N, Denmark
    Microbiology 154:383-91. 2008
    ..mutation within an otherwise identical 500 bp region of the integrase gene occurs in the viral attachment site (attP), which corresponds to the anticodon region of the targeted tRNA gene in the host chromosome...
  69. doi Severe Vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation
    Shigeo Yamaoka
    Department of Pediatrics, Perinatal Center, Osaka Medical College, Takatsuki, Osaka, Japan
    Free Radic Res 42:602-12. 2008
    ..It was concluded that severe vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress or inflammation...
  70. pmc Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi
    Yang Hong
    Department of Infectious Diseases, University of Georgia, Athens, GA, USA
    FEMS Microbiol Lett 287:63-8. 2008
    ..The transformation efficiency of vectors incorporating the PhiC31 integrase and attP sites was comparable to that of replication plasmids using the same electroporation procedure...
  71. pmc A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila
    Guanjun Gao
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 105:13999-4004. 2008
    ..In this method, which we term "site-specific integrase mediated repeated targeting" (SIRT), an attP attachment site for the phage phiC31 integrase is first targeted to the vicinity of the gene of interest by ..
  72. pmc Higher incidence of Osteonecrosis of the Jaw (ONJ) in patients with metastatic castration resistant prostate cancer treated with anti-angiogenic agents
    Jeanny B Aragon-Ching
    Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892, USA
    Cancer Invest 27:221-6. 2009
    ..patients enrolled in a phase II clinical trial combining bevacizumab, docetaxel, thalidomide, and prednisone (ATTP) in chemotherapy-naive men with metastatic castration resistant prostate cancer (mCRPC)...
  73. ncbi Targeted engineering of the Drosophila genome
    Juan Huang
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Fly (Austin) 3:274-7. 2009
    ..All three use HR-based gene targeting to first implant a single or a pair of phi C31-attP recombination sites into the target locus...
  74. doi Transgene excision from wheat chromosomes by phage phiC31 integrase
    Katja Kempe
    Leibniz Institut für Pflanzengenetik und Kulturpflanzenforschung IPK Gatersleben, Corrensstr 3, 06466, Gatersleben, Germany
    Plant Mol Biol 72:673-87. 2010
    ..express phiC31 integrase were crossed to plants carrying a target construct bearing the phiC31 recognition sites, attP and attB...
  75. doi Dietary alpha-tocopherol and neuromuscular health: search for optimal dose and molecular mechanisms continues!
    Kishorchandra Gohil
    Department of Internal Medicine, Genome and Biomedical Sciences Facility, University of California, Davis, CA 95616, USA
    Mol Nutr Food Res 54:693-709. 2010
    ..alphaT caused by mutations in either the microsomal triglyceride transfer protein or the alphaT-transfer protein (alphaTTP), underscores the necessity of alphaT for neuromuscular health in humans...
  76. doi Ataxia with vitamin E deficiency: update of molecular diagnosis
    I Di Donato
    Dipartimento di Scienze Neurologiche, Neurochirurgiche e del Comportamento, Universita degli Studi di Siena, Viale Bracci, 53100, Siena, Italy
    Neurol Sci 31:511-5. 2010
    Ataxia with vitamin E deficiency (AVED) is a rare autosomal recessive neurodegenerative disease, due to mutations in TTPA gene (Arita et al. in Biochem J 306(Pt. 2):437-443, 1995; Hentati et al...
  77. doi Characterization of the site-specific recombination system of phage ΦD145, and its capacity to promote recombination in human cells
    Sridhar Mandali
    Dept of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden
    Virology 408:64-70. 2010
    ..The minimal attB site is determined to 22 nt with 18 nt identity to the core region of attP. A non-coding sequence on the human chromosome 13 is shown to be a rather good substrate for recombination in vivo ..
  78. doi Analysis of the site-specific integration system of the Streptomyces aureofaciens phage μ1/6
    Jarmila Farkasovská
    Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Curr Microbiol 64:226-33. 2012
    ..The phage attachment site (attP) was localized downstream of the int gene...
  79. doi Genome-wide manipulations of Drosophila melanogaster with transposons, Flp recombinase, and ΦC31 integrase
    Koen J T Venken
    Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA
    Methods Mol Biol 859:203-28. 2012
    ..Similarly, transposons that contain attP attachment sites for the ΦC31 integrase can be used for numerous applications...
  80. pmc An efficient procedure for marker-free mutagenesis of S. coelicolor by site-specific recombination for secondary metabolite overproduction
    Bo Zhang
    Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China
    PLoS ONE 8:e55906. 2013
    ..markers were removed by φC31 integrase-mediated in vivo site-specific recombination between pre-placed attB and attP sites...
  81. pmc Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination
    Sean D Colloms
    Institute of Molecular, Cell and Systems Biology, University of Glasgow, Bower Building, University Avenue, Glasgow G12 8QQ, Scotland, UK, Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK, Department of Metabolic Biology, The John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK, Joint BioEnergy Institute, Emeryville, CA 94608, USA, Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    Nucleic Acids Res 42:e23. 2014
    ..Using six orthogonal attP/attB recombination site pairs with different overlap sequences, we can assemble up to five DNA fragments in a ..
  82. pmc Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells
    Yuan Yu
    1 College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi, PR China 2 Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A and F University, Yangling, Shaanxi, PR China 3
    Sci Rep 4:4240. 2014
    ..in phiC31-mediated integration in different types of mammalian cells; as a result, the efficiencies of pseudo attP site integration and evaluation of site-specific integration are compromised...
  83. pmc Hybrid lentivirus-phiC31-int-NLS vector allows site-specific recombination in murine and human cells but induces DNA damage
    Nicolas Grandchamp
    Unit of Biotechnology and Biotherapy, Centre de Recherche de l Institut du Cerveau et de la Moelle Epiniere, Pierre and Marie Curie University Institut National de la Santé et de la Recherche Médicale, Paris, France NewVectys, Villebon sur Yvette, France Biosource, Paris, France
    PLoS ONE 9:e99649. 2014
    ..to use the integrase of phage phiC31 (phiC31-int) which catalyzes efficient site-specific recombination between the attP site in the phage genome and the chromosomal attB site of its Streptomyces host...
  84. doi Targeted gene integration using the combination of a sequence-specific DNA-binding protein and phiC31 integrase
    Hideyuki Nakanishi
    Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, 46 29 Yoshida Shimoadachi cho, Sakyo ku, Kyoto 606 8501, Japan Japan Society for the Promotion of Science JSPS, Sumitomo Ichibancho FS Bldg, 8 Ichibancho, Chiyoda ku, Tokyo 102 8472, Japan
    J Biotechnol 186:139-47. 2014
    PhiC31 integrase-based vectors can integrate therapeutic genes selectively into attP or pseudo-attP sites in genomes, but considerable numbers of pseudo-attP sites in human genomes exist inside endogenous gene-coding regions...
  85. doi A GROMOS-Compatible Force Field for Small Organic Molecules in the Condensed Phase: The 2016H66 Parameter Set
    Bruno A C Horta
    Laboratory of Physical Chemistry, ETH Zurich, CH 8093 Zurich, Switzerland
    J Chem Theory Comput 12:3825-50. 2016
    ..5 kJ mol(-1), 4.1 kJ mol(-1), and 2.1 kJ mol(-1), respectively, and the corresponding average deviations (AVED) are 1.0 kg m(-3), 0.2 kJ mol(-1), 2.6 kJ mol(-1), and 1.0 kJ mol(-1), respectively...
  86. ncbi Tumor regression and growth rates determined in two intramural NCI prostate cancer trials: The growth rate constant as an indicator of therapeutic efficacy
    Bamidele Adesunloye
    Medical Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD Hebrew University, Jerusalem, Israel National Cancer Institute, National Institutes of Health, Bethesda, MD Laboratory of Tumor Immunology and Biology and Medical Oncology Branch, National Cancer Institute, Bethesda, MD Molecular Pharmacology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD National Cancer Insitute, Bethesda, MD
    J Clin Oncol 30:133. 2012
    133 Background: Like ATTP [bevacizumab + thalidomide + docetaxel + prednisone], ARTP [bevacizumab + lenalidomide + docetaxel + prednisone] is active in CRPC and in both the data suggest acquired resistance does not develop, supporting a ..
  87. ncbi Localization of alpha-tocopherol transfer protein in the brains of patients with ataxia with vitamin E deficiency and other oxidative stress related neurodegenerative disorders
    R P Copp
    Department of Medicine, New York University Medical Center, 550 First Avenue, New York, NY 10016, USA
    Brain Res 822:80-7. 1999
    ..are associated with a neurological syndrome of spinocerebellar ataxia, called ataxia with vitamin E deficiency (AVED). Earlier studies suggested that alpha-TTP is found only in the liver...
  88. pmc Genome-wide screening of alpha-tocopherol sensitive genes in heart tissue from alpha-tocopherol transfer protein null mice (ATTP(-/-))
    Vihas T Vasu
    Department of Internal Medicine, Centre for Comparative Respiratory Biology and Medicine, Genome and Biomedical Sciences Facility, Lab 6404A, 451 East Health Sciences Drive, UC Davis, Davis, CA 95616, USA
    FEBS Lett 581:1572-8. 2007
    Alpha-tocopherol transfer protein (ATTP) null mice (ATTP(-/-)) have a systemic deficiency of alpha-tocopherol (AT). The heart AT levels of ATTP(-/-) are <10% of those in ATTP(+/+) mice...
  89. ncbi [Construction of the oocyte-specific expressing phiC31 integrase vector pZP3-INT and its expression in mouse oocytes]
    Huan Yu Xu
    Shanghai Institute of Medical Genetics, Shanghai Jiaotong University, Key Laboratory of Embryo Molecular Biology, Ministry of Health, Shanghai 200040, China
    Yi Chuan 31:595-9. 2009
    ..is a site-specific recombinase, which can catalyze site-specific, unidirectional recombination between the attP site and attB site...
  90. pmc PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome
    James G Thomson
    Crop Improvement and Utilization Research Unit, Western Regional Research Center, USDA ARS, 800 Buchanan Street, Albany, CA 94710, USA
    BMC Biotechnol 10:17. 2010
    ..recombination of two recognition sites that differ in sequence, typically known as attachment sites attB and attP. Previously, we characterized the phiC31 catalytic activity and modes of action in the fission yeast ..
  91. pmc PhiC31 integrase induces efficient site-specific excision in zebrafish
    Jianjun Lu
    Vollum Institute, OHSU, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Transgenic Res 20:183-9. 2011
    ..Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination...
  92. pmc Double conditional human embryonic kidney cell line based on FLP and ΦC31 mediated transgene integration
    Christoph Waldner
    Institut für Zellbiologie Tumorforschung, Universitat Duisburg Essen, Hufelandstr, 55, D 45122 Essen, Germany
    BMC Res Notes 4:420. 2011
    ..In this study, we broaden the system of site specific integration by introducing ΦC31 integrase mediated integration into attP sites.
  93. pmc Junker: an intergenic explorer for bacterial genomes
    Jayavel Sridhar
    Centre of Excellence in Bioinformatics, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamilnadu, India
    Genomics Proteomics Bioinformatics 9:179-82. 2011
    ..units such as small regulatory RNAs, small open reading frames, pseudogenes, transposons, integrase binding attB/attP sites, repeat elements within the bacterial intergenic regions (IGRs) and in the analysis of those "junk" regions ..
  94. pmc Site-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE)
    John R Heil
    Biology, University of Waterloo, Waterloo, Canada
    J Vis Exp . 2012
    ..The ΦC31 integrase catalyzes a direct recombination between two specific DNA sites: attB and attP (34 and 39 bp, respectively). This recombination is stable and does not revert...
  95. doi Normal spermatogenesis and sperm function in a subject affected by cerebellar ataxia due to congenital vitamin E deficiency
    M Rossato
    Department of Internal Medicine, Clinica Medica 3, University of Padova, Padova, Italy
    Andrologia 46:322-4. 2014
    ..Subjects affected by cerebellar ataxia due to congenital isolated vitamin E deficiency (AVED) show vitamin E deficiency caused by a selective impaired gastrointestinal absorption of vitamin E for a mutation ..
  96. doi Fluorescent protein-based detection of φC31 integrase activity in mammalian cells
    Taian Liu
    State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Agricultural Ministry, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
    Anal Biochem 441:104-8. 2013
    ..protein (EGFP) gene and the reverse complementary DsRed gene, which are flanked by attB and reverse complementary attP. This cassette can be inverted by φC31 integrase, thereby altering the fluorescent protein expression...
  97. pmc DICE, an efficient system for iterative genomic editing in human pluripotent stem cells
    Fangfang Zhu
    Department of Genetics, Stanford University, Stanford, CA 94305, USA, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA 94305, USA, Howard Hughes Medical Institute and Department of Biology, Stanford University, Stanford, CA 94305, USA, Parkinson s Institute and Clinical Center, Sunnyvale, CA 94085, USA and Stanford Transgenic Research Facility, Stanford University, Stanford, CA 94305, USA
    Nucleic Acids Res 42:e34. 2014
    ..Recipient cell lines were established by site-specific placement of a 'landing pad' cassette carrying attP sites for phiC31 and Bxb1 integrases at the H11 locus by spontaneous or TALEN-assisted homologous recombination...
  98. pmc PhiC31 integrase induces efficient site-specific recombination in the Capra hircus genome
    Haiyan Ma
    1 Shanghai Institute of Medical Genetics, Shanghai Children s Hospital, Shanghai Jiao Tong University, Shanghai, People s Republic of China
    DNA Cell Biol 33:484-91. 2014
    ..C31 integrase induces efficient site-specific recombination capable of integrating exogenous genes at pseudo attP sites in human, mouse, rat, rabbit, sheep, Drosophila, and bovine genomes...
  99. pmc Molecular dissection of Mycobacterium tuberculosis integration host factor reveals novel insights into the mode of DNA binding and nucleoid compaction
    Narayanaswamy Sharadamma
    From the Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
    J Biol Chem 289:34325-40. 2014
    ..that wild-type mIHF, but not its DNA-binding variants, binds with high affinity to fragments containing attB and attP sites and curved DNA...
  100. pmc Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells
    Susanne Andreas
    Artemis Pharmaceuticals GmbH, Neurather Ring 1, 51063 Köln, Germany
    Nucleic Acids Res 30:2299-306. 2002
    The integrase of the phage PhiC31 recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host...
  101. ncbi Characterization of XerC- and XerD-dependent CTX phage integration in Vibrio cholerae
    Sarah M McLeod
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, Boston, MA 02111, USA
    Mol Microbiol 54:935-47. 2004
    ..cholerae chromosome. Here, we define the elements on the phage genome (attP) and bacterial chromosome (attB) required for CTXphi integration...

Research Grants34

  1. Carrie Finno; Fiscal Year: 2016
    ..Experience using mouse model will enhance Dr. Finno's future research potential. Five years of mentored support is requested. ..
  2. Functions of Tocopherol Transfer Protein
    Danny Manor; Fiscal Year: 2012
    ..The hepatic alpha tocopherol transfer protein (TTP) is a critical regulator of vitamin E status;humans carrying mutated TTP alleles develop severe ..
  3. Integration and Excision by Serine Intergrases
    Graham F Hatfull; Fiscal Year: 2013
    ..More specifically, the Bxb1 integrase acts to recombine two DNA sites - attB and attP - that are small (<50bp), different in sequence and size, and requires no other DNA or protein components...
  4. Inna Djagaeva; Fiscal Year: 2014
    ..and time consuming homologous recombination to replace the native Khc coding sequence with an enzyme-actuated attP "landing pad" ([unreadable]Khc-attP)...
  5. Recombination-mediated cassette exchange in Aedes aegypti mosquitoes
    Zach N Adelman; Fiscal Year: 2013
    ..the investigator to examine multiple transgenes in the same genetic environment, an unfortunate consequence of the attP-attB C31 system is that as a result of recombination the entire bacterial plasmid becomes integrated into the ..
  6. Hugo J Bellen; Fiscal Year: 2016
    ..Mi{MIC} contains two phage fC31 attP sites for site-specific recombination...
  7. ATTACHMENT SITE RECOGNITION IN PHAGE LAMBDA INTEGRATION
    MICHAEL BRUIST; Fiscal Year: 1991
    ..coli protein integration host factor (IHF) organizes at a DNA site, attP, on the phage chromosome. This structure, the intasome, then binds the bacterial integration site, attB...
  8. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2009
    ..on infectiousness, VEI, of vaccination, and the analogous effects of antiviral agents on infection, AVES, disease, AVED, and infectiousness, AVEI...
  9. METHODS FOR EVALUATING VACCINE EFFICACY
    M Halloran; Fiscal Year: 2005
    ..on infectiousness, VE|, of vaccination, and the analogous effects of antiviral agents on infection, AVES, disease, AVED, and infectiousness, AVE]...
  10. Oxidant induced gene expression in vitamin E deficient *
    Kishorchandra Gohil; Fiscal Year: 2002
    ..These molecular data may provide the basis for designing more effective protocols for dietary interventions with antioxidants that might ameliorate lung diseases induced by environmental oxidants. ..
  11. LIPOPROTEIN STRUCTURE/FUNCTION IN CHOLESTEROL METABOLISM
    Stephen Young; Fiscal Year: 2003
    ..The Cores (Animal Model; Histology, Atherosclerosis, Ultrastructure; Cell Culture and Protein Production; Administration) will provide the common services that are necessary for accomplishing the goals of the Program Project. ..
  12. Antioxidant Supplementation,Oxidative Stress & Enduranc*
    Maret Traber; Fiscal Year: 2002
    ..Finally, the investigators plan to measure markers of inflammatory response, such as C-oxidants increase and vitamin E decreases these markers. ..
  13. Mechanisms of Vitamin E Function Studied in Zebrafish
    Maret G Traber; Fiscal Year: 2010
    ..the molecular targets altered by - tocopherol-deficiency in the zebrafish will provide a solid basis for understanding why humans require -tocopherol for reproduction as well as to maintain a healthy nervous system ..
  14. Alpha-Tocopherol Modulation of Xenobiotic Metabolism
    Maret Traber; Fiscal Year: 2009
    ..We believe that our findings may well have a significant impact on current self-medication practices of the millions of Americans currently taking prescription drugs and vitamin E supplements. ..
  15. Oxidative Stress and Vitamin E Requirements
    Maret Traber; Fiscal Year: 2005
    ..These proposed studies will further our goal of understanding the role of vitamin E in the amelioration of oxidative damage in aging and in chronic diseases, such as heart disease, diabetes and cancer. ..
  16. Transferring integrase technology to animals
    MICHELE CALOS; Fiscal Year: 2006
    ..By scaling up to animals more similar to humans, these experiments will move the site-specific integrase technology closer to the clinic. [unreadable] [unreadable] [unreadable]..
  17. Phage mimicry of mycobacterial signaling
    Graham F Hatfull; Fiscal Year: 2010
    ..We propose to elucidate the specific role of GroEL1 in biofilm formation and mycolic acid synthesis, and to determine its role in the pathogenesis of Mycobacterium tuberculosis. ..
  18. Site-specific transgenesis in zebrafish
    JAMES LISTER; Fiscal Year: 2008
    ..The phiC31 integrase promotes recombination between an attachment site in the phage (attP) and another on the bacterial chromosome (attB)...
  19. Mycobacteriophage-mediated Recombineering in Mycobacterium tuberculosis
    Graham Hatfull; Fiscal Year: 2007
    ..These putative recombination proteins will be functionally and biochemically characterized and used to develop recombineering systems in both fast- and slow-growing mycobacteria. ..
  20. Retinal Gene Therapy by Site-Specific Integration
    MICHELE CALOS; Fiscal Year: 2006
    ..These experiments will move the phiC31 integrase [unreadable] [unreadable] [unreadable]..
  21. Inhibitors of pox virus Holliday juction resolvase
    Anca Segall; Fiscal Year: 2005
    ....
  22. Human immunotherapeutics for Biodefense Applications
    Philip Leighton; Fiscal Year: 2004
    ..abstract_text> ..
  23. MECHANISM OF PHAGE L5 INTEGRATION AND EXCISION
    Graham Hatfull; Fiscal Year: 2002
    ..by the phage-encoded integrase protein which mediates site-specific recombination between the phage attachment, attP, and the chromosomal attachment site, attB...
  24. Membrane Proteins and Mycoplasma arthritidis
    LEIGH WASHBURN; Fiscal Year: 2006
    ..arthritidis-induced arthritis. This, in turn, will provide insight into how similar events may occur in inflammatory joint diseases of humans and other animals. [unreadable] [unreadable]..
  25. MOLECULAR BIOLOGY OF MYCOBACTERIA AND THEIR PHAGES
    Graham Hatfull; Fiscal Year: 2004
    ..We will also use proteomic approaches to understand the influence of phage gene expression on that of its host. ..
  26. Characterization of Acid Induced Promoters of M. tb
    Beatrice Saviola; Fiscal Year: 2004
    ..This work is significant because it will contribute to a greater understanding of mycobacterial stress response and ultimately survival and persistence in the host during infection. ..
  27. Pathways fo Lambda Site-Specific Recombination
    ANCA MARA SEGALL; Fiscal Year: 2010
    ..The new characterization will include structural studies of the inhibitors. ..
  28. Custom integration tools for functional genomics
    MICHELE CALOS; Fiscal Year: 2003
    ..in my laboratory involving recombination mediated by phage integrases at their compact (30 - 40-bp) attB and attP recognition sites...
  29. SITE-SPECIFIC INTEGRATION FOR GENE THERAPY
    MICHELE CALOS; Fiscal Year: 2003
    ..The novel site-specific integration strategy developed here can be used to add value to most current gene therapy vectors. ..
  30. INTEGRATION AND EXCISION OF PROPHAGES IN TUBERCULOSIS
    Graham Hatfull; Fiscal Year: 2003
    ..tuberculoses to determine whether they have functional recombination systems; whether they act as Genetic Transfer Agents; and whether they play a role in virulence of M. tuberculosis. ..
  31. Human Polyclonal Antibody Production in Chickens
    Philip Leighton; Fiscal Year: 2002
    ..The proposed technology will offer several advantages over current antibody production methods and thus will easily capture a share of this market. ..
  32. Novel antibiotics that trap Holliday junctions
    Anca Segall; Fiscal Year: 2008
    ..6) Investigate the effect of the peptides on Salmonella and Staph infections in mice in two well-studied infectious disease model systems. ..