alpha-actinin

Summary

Gene Symbol: alpha-actinin
Description: actinin alpha 1
Alias: BDPLT15, alpha-actinin-1, F-actin cross-linking protein, actinin 1 smooth muscle, alpha-actinin cytoskeletal isoform, non-muscle alpha-actinin-1
Species: human

Top Publications

  1. ncbi Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines
    Hiroyuki Sakagami
    Division of Histology, Department of Cell Biology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Sendai 980 8575, Japan
    Eur J Neurosci 25:618-28. 2007
  2. doi Alpha-actinin-4 is selectively required for insulin-induced GLUT4 translocation
    Ilana Talior-Volodarsky
    Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    J Biol Chem 283:25115-23. 2008
  3. pmc An interaction between alpha-actinin and the beta 1 integrin subunit in vitro
    C A Otey
    Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599
    J Cell Biol 111:721-9. 1990
  4. pmc The cDNA sequence of a human placental alpha-actinin
    D B Millake
    Department of Biochemistry, University of Leicester, UK
    Nucleic Acids Res 17:6725. 1989
  5. ncbi An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment
    M Reinhard
    Institute of Clinical Biochemistry and Pathobiochemistry, University of Wurzburg, Versbacher Strasse 5, D 97078 Wurzburg, Germany
    J Biol Chem 274:13410-8. 1999
  6. ncbi CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4
    T Vallenius
    Haartman Institute and Biocentrum Helsinki, University of Helsinki, 00014 Helsinki, Finland
    J Biol Chem 275:11100-5. 2000
  7. ncbi Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein
    M Edlund
    Department of Cell Biology, University of Virginia, Charlottesville, USA
    Cell Motil Cytoskeleton 48:190-200. 2001
  8. ncbi Interactions of a hemidesmosome component and actinin family members
    A M Gonzalez
    Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E Chicago Avenue, Chicago, IL 60611, USA
    J Cell Sci 114:4197-206. 2001
  9. doi Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells
    Quincy Quick
    Department of Biology, Grambling State University, Grambling, LA 71245, USA
    Exp Cell Res 316:1137-47. 2010
  10. pmc ACTN1 mutations cause congenital macrothrombocytopenia
    Shinji Kunishima
    Department of Advanced Diagnosis, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan
    Am J Hum Genet 92:431-8. 2013

Research Grants

  1. Richard A Firtel; Fiscal Year: 2015
  2. Role of Clic in Epithelial Morphogenesis
    Mark Berryman; Fiscal Year: 2008

Scientific Experts

Detail Information

Publications181 found, 100 shown here

  1. ncbi Somatodendritic localization of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6, and its possible interaction with alpha-actinin in dendritic spines
    Hiroyuki Sakagami
    Division of Histology, Department of Cell Biology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Sendai 980 8575, Japan
    Eur J Neurosci 25:618-28. 2007
    ..Our present findings suggest that EFA6A may form a protein complex with alpha-actinin and activate ARF6 in close proximity of the actin cytoskeleton and membrane proteins in the dendritic spines...
  2. doi Alpha-actinin-4 is selectively required for insulin-induced GLUT4 translocation
    Ilana Talior-Volodarsky
    Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada
    J Biol Chem 283:25115-23. 2008
    ..We propose that ACTN4 contributes to GLUT4 traffic, likely by tethering GLUT4 vesicles to the cortical actin cytoskeleton...
  3. pmc An interaction between alpha-actinin and the beta 1 integrin subunit in vitro
    C A Otey
    Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599
    J Cell Biol 111:721-9. 1990
    ..These results lead us to suggest that this integrin-alpha-actinin linkage may contribute to the attachment of actin filaments to the membrane in certain locations...
  4. pmc The cDNA sequence of a human placental alpha-actinin
    D B Millake
    Department of Biochemistry, University of Leicester, UK
    Nucleic Acids Res 17:6725. 1989
  5. ncbi An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment
    M Reinhard
    Institute of Clinical Biochemistry and Pathobiochemistry, University of Wurzburg, Versbacher Strasse 5, D 97078 Wurzburg, Germany
    J Biol Chem 274:13410-8. 1999
    ..Our results suggest a crucial role for the alpha-actinin-zyxin interaction in subcellular zyxin localization and microfilament organization...
  6. ncbi CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4
    T Vallenius
    Haartman Institute and Biocentrum Helsinki, University of Helsinki, 00014 Helsinki, Finland
    J Biol Chem 275:11100-5. 2000
    ..More generally, results presented here demonstrate that the association of PDZ-LIM proteins with the cytoskeleton extends to the actin stress fibers of nonmuscle cells...
  7. ncbi Dynamics of alpha-actinin in focal adhesions and stress fibers visualized with alpha-actinin-green fluorescent protein
    M Edlund
    Department of Cell Biology, University of Virginia, Charlottesville, USA
    Cell Motil Cytoskeleton 48:190-200. 2001
    ..Additionally, the exchange rates revealed by video-microscopy and FRAP analysis of alpha AGFP are more rapid than those reported previously, which were obtained using microinjection of large excesses of fluorescently-tagged protein...
  8. ncbi Interactions of a hemidesmosome component and actinin family members
    A M Gonzalez
    Department of Cell and Molecular Biology, Northwestern University Medical School, 303 E Chicago Avenue, Chicago, IL 60611, USA
    J Cell Sci 114:4197-206. 2001
    ..We discuss a role for BP180 in crosstalk between cell-matrix and cell-cell junctions...
  9. doi Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells
    Quincy Quick
    Department of Biology, Grambling State University, Grambling, LA 71245, USA
    Exp Cell Res 316:1137-47. 2010
    ....
  10. pmc ACTN1 mutations cause congenital macrothrombocytopenia
    Shinji Kunishima
    Department of Advanced Diagnosis, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan
    Am J Hum Genet 92:431-8. 2013
    ..Our findings provide an insight into the pathogenesis of CMTP...
  11. ncbi A fragment of alpha-actinin promotes monocyte/macrophage maturation in vitro
    S Luikart
    Veterans Affairs Medical Center, Department of Medicine, University of Minnesota, Minneapolis 55417, USA
    Exp Hematol 27:337-44. 1999
    ..This represents the first example of a fragment of a cytoskeletal component, which may be released during tissue remodeling and repair, playing a role in phagocyte maturation...
  12. ncbi Structure of the alpha-actinin-vinculin head domain complex determined by cryo-electron microscopy
    Deborah F Kelly
    Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306 4380, USA
    J Mol Biol 357:562-73. 2006
    ..The results show that the interaction between two species with weak affinity can be visualized in a membrane-like environment...
  13. ncbi Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells
    U Gluck
    Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel
    J Cell Sci 107:1773-82. 1994
    ....
  14. ncbi Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins
    Vincent C Chen
    Department of Chemistry, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada
    J Proteome Res 5:2123-34. 2006
    ....
  15. doi RelA/NF-kappaB transcription factor associates with alpha-actinin-4
    Vladimir N Babakov
    Department of Cell Cultures, Institute of Cytology, St Petersburg, Russian Federation
    Exp Cell Res 314:1030-8. 2008
    ..We suggest that the alpha-actinin-4 is important for the NF-kappaB nuclear translocation and its functions inside the nucleus...
  16. ncbi Prognostic significance of drug-regulated genes in high-grade osteosarcoma
    Joerg Fellenberg
    Department of Experimental Orthopedics, Orthopedic Hospital, University of Heidelberg, Heidelberg, Germany
    Mod Pathol 20:1085-94. 2007
    ..Owing to their involvement in drug action these genes may further be potential targets for the modulation of drug sensitivity...
  17. pmc Monocyte protein signatures of disease severity in sickle cell anemia
    Anita Hryniewicz-Jankowska
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75080, USA
    Exp Biol Med (Maywood) 234:210-21. 2009
    ..correlated with crisis rate in the cytoplasmic fraction were far upstream element-binding protein and Alpha actinin 1 or Alpha actinin 4...
  18. doi Kinetic behaviour of WT 1's zinc finger domain in binding to the alpha-actinin-1 mRNA
    Elmar Nurmemmedov
    Molecular Biophysics, Chemical Center, Lund University, 221 00 Lund, Sweden
    Arch Biochem Biophys 497:21-7. 2010
    ..The stem-loop and the flanking nucleotides are important elements for specific recognition by WT 1 zinc fingers...
  19. doi Actin stress fiber pre-extension in human aortic endothelial cells
    Lan Lu
    Department of Biomedical Engineering, Washington University, St Louis, Missouri 63130, USA
    Cell Motil Cytoskeleton 65:281-94. 2008
    ..Unlike pre-extension, the morphology of the compressed SFs is partially determined by MTs and IFs which appear to support the SFs along their lengths...
  20. pmc Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell
    Paula Stanley
    Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, London, UK
    EMBO J 27:62-75. 2008
    ..Identification of distinctive roles for intermediate- and high-affinity LFA-1 in T lymphocyte migration provides a biological function for two active conformations of this integrin for the first time...
  21. pmc Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P
    Max Koltzscher
    Institute for Medical Biochemistry, University of Muenster, Germany
    Mol Biol Cell 14:2372-84. 2003
    ..This identifies S100P as a novel activator of ezrin and indicates that activation of ezrin's cross-linking function can occur directly in response to Ca2+ transients...
  22. pmc Memo-RhoA-mDia1 signaling controls microtubules, the actin network, and adhesion site formation in migrating cells
    Kossay Zaoui
    French National Institute for Health and Medical Research Unit 891, Centre de Recherche en Cancerologie de Marseille, 13009 Marseille, France
    J Cell Biol 183:401-8. 2008
    ....
  23. pmc Development of a multiplex quantitative PCR signature to predict progression in non-muscle-invasive bladder cancer
    Rou Wang
    Department of Urology, Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109 0944, USA
    Cancer Res 69:3810-8. 2009
    ..Thus, we identified a 57-gene QPCR panel to help predict progression of non-muscle-invasive bladder cancers and delineate a systematic, generalizable approach to converting microarray data into a multiplex assay for cancer progression...
  24. pmc α-actinin-4 is essential for maintaining the spreading, motility and contractility of fibroblasts
    Hanshuang Shao
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e13921. 2010
    ..Interestingly, ACTN4 is phosphorylated upon growth factor stimulation, and this loosens its interaction with actin...
  25. pmc Molecular structure of the sarcomeric Z-disk: two types of titin interactions lead to an asymmetrical sorting of alpha-actinin
    P Young
    European Molecular Biology Laboratory, Postfach 10 22 09, 69012 Heidelberg, Germany
    EMBO J 17:1614-24. 1998
    ....
  26. ncbi Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes
    Thomas Nebl
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 277:43399-409. 2002
    ..We suggest that the DRM-H fraction represents a membrane skeleton-associated subset of leukocyte signaling domains...
  27. ncbi Phosphorylation-dependent interactions of alpha-Actinin-1/IQGAP1 with the AMPA receptor subunit GluR4
    Mutsuo Nuriya
    Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurochem 95:544-52. 2005
    ..In addition, our data indicate that activity-dependent phosphorylation of GluR4 may regulate its synaptic targeting through phosphorylation-dependent interactions with alpha-Actinin-1 and IQGAP1...
  28. pmc Phosphoinositide binding regulates alpha-actinin CH2 domain structure: analysis by hydrogen/deuterium exchange mass spectrometry
    Stephen J Full
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    Protein Sci 16:2597-604. 2007
    ....
  29. pmc SiRNA-mediated reduction of alpha-actinin-1 inhibits pressure-induced murine tumor cell wound implantation and enhances tumor-free survival
    David H Craig
    Department of Surgery, John D Dingell VA Medical Center and Wayne State University, Detroit, MI 48201, USA
    Neoplasia 10:217-22. 2008
    ..alpha-Actinin-1 may be an effective therapeutic target to inhibit perioperative pressure-stimulated tumor cell implantation...
  30. doi Insight into the neuroproteomics effects of the food-contaminant non-dioxin like polychlorinated biphenyls
    Laura Brunelli
    Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy
    J Proteomics 75:2417-30. 2012
    ..As such, our work is expected to lead to new insights into the mechanisms of NDL-PCBs neurotoxicity...
  31. doi Pathogenesis and management of inherited thrombocytopenias: rationale for the use of thrombopoietin-receptor agonists
    Alessandro Pecci
    Department of Internal Medicine, IRCCS Policlinico San Matteo Foundation, University of Pavia, Piazzale Golgi, 27100 Pavia, Italy
    Int J Hematol 98:34-47. 2013
    ....
  32. pmc Genome wide proteomics of ERBB2 and EGFR and other oncogenic pathways in inflammatory breast cancer
    Emma Yue Zhang
    Barnett Institute and Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, Massachusetts 02115, United States
    J Proteome Res 12:2805-17. 2013
    ..Future studies of inflammatory breast cancer (IBC), from which the cell lines were derived, will be used to explore the significance of these observations...
  33. pmc Urinary candidate biomarker discovery in a rat unilateral ureteral obstruction model
    Yuan Yuan
    Department of Pathophysiology, National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China
    Sci Rep 5:9314. 2015
    ....
  34. doi Trypanosoma cruzi extracellular amastigotes trigger the protein kinase D1-cortactin-actin pathway during cell invasion
    Alexis Bonfim-Melo
    Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo EPM UNIFESP, Sao Paulo, Brazil
    Cell Microbiol 17:1797-810. 2015
    ..Collectively, these results show new host cell mechanisms subverted during EA internalization into non-phagocytic cells. ..
  35. pmc Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites
    Wei Ke Ji
    Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, United States
    elife 4:e11553. 2015
    ..We propose that Drp1 is in dynamic equilibrium on mitochondria in a fission-independent manner, and that fission factors such as actin filaments target productive oligomerization to fission sites. ..
  36. pmc Interactions between the Microtubule Binding Protein EB1 and F-Actin
    Emily O Alberico
    Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, 46556, USA
    J Mol Biol 428:1304-14. 2016
    ....
  37. pmc Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse
    Irina V Ogneva
    Department of Molecular and Cell Biomedicine, State Scientific Center of Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia
    PLoS ONE 11:e0153650. 2016
    ..Thus, lecithin prevented the reduction of Actn1 and Actn4 mRNA and the migration of ACTN4 from the cortical cytoskeleton to the cytoplasm...
  38. pmc Cardiomyocyte-Specific Ablation of Med1 Subunit of the Mediator Complex Causes Lethal Dilated Cardiomyopathy in Mice
    Yuzhi Jia
    Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America
    PLoS ONE 11:e0160755. 2016
    ....
  39. ncbi Microtubule-associated proteins, MAP 1A and MAP 1B, interact with F-actin in vitro
    T Fujii
    Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Nagano
    J Biochem 114:827-9. 1993
    ....
  40. pmc The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis
    W Witke
    Max Planck Institute for Biochemistry, Martinsried, Germany
    J Cell Biol 121:599-606. 1993
    ....
  41. ncbi The neural cell adhesion molecule is associated with major components of the cytoskeleton
    Bettina Büttner
    Institut fur Molekularbiologie und Biochemie, Charite Universitatsmedizin Berlin, Berlin Dahlem, Germany
    Biochem Biophys Res Commun 310:967-71. 2003
    ..Furthermore, we demonstrate that inhibition of rhoA-binding kinase-alpha stimulates neurite outgrowth independently from NCAM...
  42. ncbi Differential subcellular targeting of PKC-epsilon in response to pharmacological or ischemic stimuli in intestinal epithelia
    Joshua M V Mammen
    Epithelial Pathobiology Research Group, Dept of Surgery, University of Cincinnati College of Medicine, PO Box 670558, Cincinnati, OH 45267 0558, USA
    Am J Physiol Gastrointest Liver Physiol 288:G135-42. 2005
    ..The data suggest that the differential translocation of PKC-epsilon in response to pharmacological agonists versus ischemic stress may lead to different effects on downstream targets...
  43. ncbi Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton
    Mikko Rönty
    Biomedicum Helsinki, Department of Pathology and Neuroscience Program, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
    Exp Cell Res 310:88-98. 2005
    ..The interactions provide an explanation to the previously observed Z-disc-specific localization of ArgBP2 and indicate interplay between signaling adaptors and structural proteins of the Z-disc...
  44. pmc Alternative splicing and differential gene expression in colon cancer detected by a whole genome exon array
    Paul J Gardina
    Affymetrix, Inc, Santa Clara, CA 95051, USA
    BMC Genomics 7:325. 2006
    ..0 ST) that allows genome-wide identification of differential splice variation, and concurrently provides a flexible and inclusive analysis of gene expression...
  45. doi Alternative splicing in colon, bladder, and prostate cancer identified by exon array analysis
    Kasper Thorsen
    Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK 8200 Aarhus N, Denmark
    Mol Cell Proteomics 7:1214-24. 2008
    ....
  46. pmc Mactinin, a fragment of cytoskeletal alpha-actinin, is a novel inducer of heat shock protein (Hsp)-90 mediated monocyte activation
    Sharon D Luikart
    Hematology Oncology Section 111E, Veterans Affairs Medical Center, Minneapolis, MN 55417, USA
    BMC Cell Biol 10:60. 2009
    ..We therefore sought to determine the mechanism by which mactinin stimulates monocytes...
  47. pmc Cell membrane expression of cardiac sodium channel Na(v)1.5 is modulated by alpha-actinin-2 interaction
    Rahima Ziane
    Centre de Recherche Universite Laval Robert Giffard, Quebec City, QC, Canada
    Biochemistry 49:166-78. 2010
    ..Our data suggest that alpha-actinin-2, which is known to regulate the functional expression of the potassium channels, may play a role in anchoring Na(v)1.5 to the membrane by connecting the channel to the actin cytoskeleton network...
  48. pmc Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology
    Shamik Sen
    Department of Bioengineering, University of California, Berkeley, California, USA
    PLoS ONE 4:e8427. 2009
    ....
  49. pmc Highly efficient purification of protein complexes from mammalian cells using a novel streptavidin-binding peptide and hexahistidine tandem tag system: application to Bruton's tyrosine kinase
    Yifeng Li
    Department of Anesthesiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Protein Sci 20:140-9. 2011
    ....
  50. doi α-Actinin-3 deficiency is associated with reduced bone mass in human and mouse
    Nan Yang
    Institute for Neuroscience and Muscle Research, The Children s Hospital at Westmead, Sydney 2145, NSW, Australia
    Bone 49:790-8. 2011
    ..Given the high frequency of R577X in the general population, the potential role of ACTN3 R577X as a factor influencing variations in BMD in elderly humans warrants further study...
  51. doi Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells
    Sabine Windhorst
    Institut für Biochemie und Molekularbiologie I Zelluläre Signaltransduktion, Universitatsklinikum Hamburg Eppendorf, Martinistr 52, D 20246 Hamburg, Germany
    Int J Cancer 129:1300-9. 2011
    ..Therefore, we suggest that ITPKA may be a promising therapeutic molecular target for anti metastatic therapy of lung cancer...
  52. pmc Alpha actinin is specifically recognized by Multiple Sclerosis autoantibodies isolated using an N-glucosylated peptide epitope
    Shashank Pandey
    Laboratory of Peptide and Protein Chemistry and Biology, University of Florence, Via della Lastruccia 13, I 50019 Sesto Fiorentino, Italy
    Mol Cell Proteomics 12:277-82. 2013
    ..and MS/MS techniques, revealed the presence of four proteins distinguishable by their mass: alpha fodrin, alpha actinin 1, creatine kinase, and CNPase...
  53. pmc α-actinin1 and 4 tyrosine phosphorylation is critical for stress fiber establishment, maintenance and focal adhesion maturation
    Yunfeng Feng
    Departments of Medicine, Washington University, St Louis, MO 63110, USA
    Exp Cell Res 319:1124-35. 2013
    ..Further rescue experiments demonstrate that the tyrosine phosphorylation of α-actinin1 at Y12 and α-actinin4 at Y265 is critical for dorsal stress fiber establishment, transverse arc maintenance and focal adhesion maturation...
  54. doi Deletions in 14q24.1q24.3 are associated with congenital heart defects, brachydactyly, and mild intellectual disability
    Barbara Oehl-Jaschkowitz
    Practice of Human Genetics, Homburg Saar, Germany
    Am J Med Genet A 164:620-6. 2014
    ....
  55. pmc Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity
    Irina V Ogneva
    Department of Molecular and Cell Biomedicine, State Scientific Center of Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia
    PLoS ONE 9:e96395. 2014
    ....
  56. pmc RBFOX1 cooperates with MBNL1 to control splicing in muscle, including events altered in myotonic dystrophy type 1
    Roscoe Klinck
    Department of Microbiology and Infectiology, Faculty of Medicine and Heath Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, Canada Laboratory of Functional Genomics, Faculty of Medicine and Heath Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, Canada
    PLoS ONE 9:e107324. 2014
    ..Our results suggest that reduced RBFOX1 activity in DM1 tissues may amplify several of the splicing alterations caused by the deficiency in MBNL1. ..
  57. pmc Arg kinase-binding protein 2 (ArgBP2) interaction with α-actinin and actin stress fibers inhibits cell migration
    Praju Vikas Anekal
    From the sGSK Group Institute of Molecular and Cell Biology IMCB, Proteos Building, 61 Biopolis Drive, 138673 Singapore
    J Biol Chem 290:2112-25. 2015
    ..ArgBP2 is known to be down-regulated in some aggressively metastatic cancers. Our work provides a biochemical explanation for the anti-migratory effect of ArgBP2...
  58. doi Rcan1 deficiency impairs neuronal migration and causes periventricular heterotopia
    Yang Li
    Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai 200031, People s Republic of China, and University of Chinese Academy of Sciences, Shanghai 200031, People s Republic of China
    J Neurosci 35:610-20. 2015
    ..Together, these findings demonstrate that Rcan1 acts upstream from Flna in regulating radial migration and suggest that impairment of Rcan1-Flna pathway may underlie PH pathogenesis. ..
  59. pmc Post-Transcriptional Regulation of KLF4 by High-Risk Human Papillomaviruses Is Necessary for the Differentiation-Dependent Viral Life Cycle
    Vignesh Kumar Gunasekharan
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America
    PLoS Pathog 12:e1005747. 2016
    ..KLF4 has also been shown to be a critical regulator of lytic Epstein Barr virus (EBV) replication underscoring the importance of this cellular transcription factor in the life cycles of multiple human cancer viruses. ..
  60. pmc Supervillin Is a Component of the Hair Cell's Cuticular Plate and the Head Plates of Organ of Corti Supporting Cells
    Lana M Pollock
    Department of Otolaryngology Head and Neck Surgery, Case Western Reserve University, Cleveland, Ohio, 44106, United States of America
    PLoS ONE 11:e0158349. 2016
    ..Overall, supervillin localization suggests this protein may shape the surface structure of the organ of Corti. ..
  61. pmc Celecoxib exhibits an anti-gastric cancer effect by targeting focal adhesion and leukocyte transendothelial migration-associated genes
    Guo Hua Jin
    Department of Gastroenterology, The First Hospital of Jilin University, Changchun, Jilin 130021, P R China
    Oncol Lett 12:2345-2350. 2016
    ..However, relevant experiments are required to confirm the conclusion of the present study...
  62. pmc A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor
    M Brink
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    J Cell Biol 111:1477-89. 1990
    ..Our results indicate that control of cell shape and motility does not require the fine-tuned interactions of all proteins that have been identified as actin-binding proteins by in vitro assays...
  63. ncbi Inhibitory effects of poly(L-aspartic acid) on the assembly of brain microtubules and the interaction of microtubule-associated protein 2 with F-actin in vitro
    A Nakamura
    Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University, Nagano
    J Biochem 106:93-7. 1989
    ..These results suggest that PLAA suppressed the functions of MAP 2 through a domain which is located in the 35 kDa fragment...
  64. ncbi Functional dissection of nebulette demonstrates actin binding of nebulin-like repeats and Z-line targeting of SH3 and linker domains
    C L Moncman
    Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, USA
    Cell Motil Cytoskeleton 44:1-22. 1999
    ..Our data also indicate that the linker and SH3 domains can distinguish between dense bodies and Z-lines, suggesting that the ligands for their interactions are specific to these muscular substructures...
  65. ncbi Intracellular domains of NR2 alter calcium-dependent inactivation of N-methyl-D-aspartate receptors
    Bryce Vissel
    Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, California, USA
    Mol Pharmacol 61:595-605. 2002
    ..We speculate that the M2-3 loop of NR2 modulates calcium-dependent inactivation by interacting with the NR1 C terminus, a region known to be essential for inactivation...
  66. ncbi Brain-specific splicing of alpha-actinin 1 (ACTN1) mRNA
    Joachim Kremerskothen
    Department for Experimental Tumorbiology, University Muenster, Badestrasse 9, Germany
    Biochem Biophys Res Commun 295:678-81. 2002
    ..In situ hybridization analysis revealed that BS expression was highest in neurons of the hippocampus, cortex, and caudate putamen while the cerebellum and other subcortical structures showed only weak labeling...
  67. ncbi Fibroblast growth factor receptor signaling promotes radial glial identity and interacts with Notch1 signaling in telencephalic progenitors
    Keejung Yoon
    Institute for Cell Engineering, Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 24:9497-506. 2004
    ....
  68. ncbi Proteomic signatures corresponding to histological classification and grading of soft-tissue sarcomas
    Yoshiyuki Suehara
    Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo, Japan
    Proteomics 6:4402-9. 2006
    ....
  69. ncbi Evidence of a functional role for interaction between ICAM-1 and nonmuscle alpha-actinins in leukocyte diapedesis
    Lionel Celli
    Institut National de la Sante et de la Recherche Medicale, unité 578, Grenoble, France, and Université Grenoble I, Groupe de Recherche sur le Cancer du Poumon, Institut Albert Bonniot, Grenoble, France
    J Immunol 177:4113-21. 2006
    ..Thus, our data demonstrate that ICAM-1 directly interacts with cytoplasmic alpha-actinin 1 and 4 and that this interaction is required for leukocyte extravasation...
  70. ncbi Alpha-actinin 4 potentiates myocyte enhancer factor-2 transcription activity by antagonizing histone deacetylase 7
    Sharmistha Chakraborty
    Department of Biochemistry, School of Medicine, Case Western Reserve University, and the Research Institute of University Hospitals of Cleveland, OH 44106, USA
    J Biol Chem 281:35070-80. 2006
    ..MEF2A association and enhancement of MEF2-mediated transcription. Taken together, we have identified a novel mechanism by which HDAC7 activity is negatively regulated and uncovered a previously unknown function of alpha-actinin 4...
  71. ncbi Filamin-regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium
    Nandkumar Khaire
    Institut fur Biochemie I, Zentrum Molekulare Medizin Köln, Medizinische Fakultat, Universitat zu Koln, 50931 Koln, Germany
    J Biol Chem 282:1948-55. 2007
    ..Likewise, in chimeric slugs wild type cells were primarily found at the tip of the slug, which acts as an organizer in Dictyostelium morphogenesis...
  72. ncbi Clusterin isoforms differentially affect growth and motility of prostate cells: possible implications in prostate tumorigenesis
    Roberta M Moretti
    Institute of Endocrinology, University of Milan, Milan, Italy
    Cancer Res 67:10325-33. 2007
    ..Thus, the antimotility activity of nCLU and its ability to cause dismantling of the actin cytoskeleton seem to be mediated by its binding to alpha-actinin...
  73. pmc The cytoskeletal protein alpha-actinin regulates acid-sensing ion channel 1a through a C-terminal interaction
    Mikael K Schnizler
    Howard Hughes Medical Institute and Department of Internal Medicine, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    J Biol Chem 284:2697-705. 2009
    ..These findings suggest that alpha-actinins may link ASIC1a to a macromolecular complex in the postsynaptic membrane where it regulates ASIC1a activity...
  74. pmc Filamin A regulates caveolae internalization and trafficking in endothelial cells
    Maria Sverdlov
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    Mol Biol Cell 20:4531-40. 2009
    ..Taken together, these data suggest that filamin A association with caveolin-1 promotes caveolae-mediated transport by regulating vesicle internalization, clustering, and trafficking...
  75. pmc Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates
    Christopher K Holterhoff
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251, USA
    Dev Dyn 238:2936-47. 2009
    ..Duplication of actn3 in the zebrafish indicates that variation in actn3 expression may promote physiological diversity in muscle function among vertebrates...
  76. doi The role of superoxide anion in the inhibitory effect of SIN-1 in thrombin-activated human platelet adhesion
    Marcia H M Cardoso
    Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, P O Box 6111, 13084 971 Campinas, Brazil
    Eur J Pharmacol 627:229-34. 2010
    ..Our data show that platelet adhesion inhibition by SIN-1 in activated platelets involves cGMP-independent mechanism through O(2)(-) generation. Superoxide anion signaling pathway includes ONOO(-) formation and alpha-actinin nitration...
  77. pmc Phosphorylation of alpha-actinin 4 upon epidermal growth factor exposure regulates its interaction with actin
    Hanshuang Shao
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 285:2591-600. 2010
    ..These results suggest that EGF regulates the actin binding activity of ACTN4 by inducing tyrosyl-directed phosphorylation...
  78. pmc Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis
    Parveen Sharma
    Department of Physiology, University of Toronto, Toronto, Ontario, Canada
    Mol Biosyst 7:1185-96. 2011
    ..Since proteins assemble into dynamic complexes within the cell, FHL1 likely mediates its biological functions in conjunction with other proteins. Delineation of FHL1 interactions could provide insight into its regulatory functions...
  79. pmc HAMLET binding to α-actinin facilitates tumor cell detachment
    Maria Trulsson
    Institute of Laboratory Medicine, Department of Microbiology, Immunology and Glycobiology, Lund University, Lund, Sweden
    PLoS ONE 6:e17179. 2011
    ..As α-actinins also associate with signaling molecules, cytoplasmic domains of transmembrane receptors and ion channels, additional α-actinin-dependent mechanisms are discussed...
  80. doi Localization of the invadopodia-related proteins actinin-1 and cortactin to matrix-contact-side cytoplasm of cancer cells in surgically resected lung adenocarcinomas
    Shinichi Hirooka
    Department of Pathology, Tokyo Medical and Dental University, Japan
    Pathobiology 78:10-23. 2011
    ..We investigated an alteration of subcellular localization of invadopodia-related actin-associated proteins, actinin-1 and cortactin, in lung adenocarcinomas, its clinical significance, and its possible regulatory factors...
  81. pmc Familial focal segmental glomerulosclerosis (FSGS)-linked α-actinin 4 (ACTN4) protein mutants lose ability to activate transcription by nuclear hormone receptors
    Simran Khurana
    Department of Biochemistry, School of Medicine, Case Western Reserve University and Research Institute of University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
    J Biol Chem 287:12027-35. 2012
    ..Our data provide the first link between FSGS-linked ACTN4 mutants and transcriptional activation by nuclear receptor such as RARα and peroxisome proliferator-activated receptor γ...
  82. doi Assembly of non-contractile dorsal stress fibers requires α-actinin-1 and Rac1 in migrating and spreading cells
    Bianca Kovac
    Genome Scale Biology Program, University of Helsinki, P O Box 63, Helsinki 00014, Finland
    J Cell Sci 126:263-73. 2013
    ..Furthermore, we propose that non-contractile dorsal stress fibers promote cell migration and early cell spreading through Rac1-induced actin polymerization...
  83. pmc Response gene to complement 32 protein promotes macrophage phagocytosis via activation of protein kinase C pathway
    Rui Tang
    From the Department of Physiology and Pharmacology, University of Georgia, Athens, Georgia 30602 and
    J Biol Chem 289:22715-22. 2014
    ..Taken together, our results demonstrate for the first time that RGC-32 is a novel membrane regulator for macrophage phagocytosis. ..
  84. pmc α-Actinin-4 enhances colorectal cancer cell invasion by suppressing focal adhesion maturation
    Miki Fukumoto
    Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe, Hyogo 650 0017, Japan
    PLoS ONE 10:e0120616. 2015
    ..Altogether, our results suggest that ZYX defective binding to ACTN4, which occupies focal adhesions instead of ACTN1, induces the formation of immature focal adhesions, resulting in the enhancement of cell motility and invasion. ..
  85. doi A translocation t(6;14) in two cases of leiomyosarcoma: Molecular cytogenetic and array-based comparative genomic hybridization characterization
    Marieke A de Graaff
    Department of Pathology, Leiden University Medical Center LUMC, Leiden, The Netherlands
    Cancer Genet 208:537-44. 2015
    ..The other identified breakpoints appeared to be non-recurrent, because they were not detected in other leiomyosarcomas, uterine leiomyomas, undifferentiated spindle cell sarcomas, or undifferentiated pleomorphic sarcomas. ..
  86. pmc Genome-wide Association Study of Platelet Count Identifies Ancestry-Specific Loci in Hispanic/Latino Americans
    Ursula M Schick
    Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA Genetics of Obesity and Related Metabolic Traits Program, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
    Am J Hum Genet 98:229-42. 2016
    ..Our results underscore the value of diverse populations to extend insights into the allelic architecture of complex traits. ..
  87. doi Congenital macrothrombocytopenia-linked mutations in the actin-binding domain of α-actinin-1 enhance F-actin association
    Anita C H Murphy
    School of Biochemistry and Cell Biology, University College Cork, Ireland
    FEBS Lett 590:685-95. 2016
    ....
  88. ncbi RhoA binds to the amino terminus of MEKK1 and regulates its kinase activity
    Ewen D Gallagher
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9041, USA
    J Biol Chem 279:1872-7. 2004
    ..In summary, we have characterized a novel point at which Rho GTPases impinge upon the regulation and function of MEKK1...
  89. ncbi Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding models
    Emma Borrego-Diaz
    Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
    J Struct Biol 155:230-8. 2006
    ....
  90. ncbi Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components
    Brian P Head
    Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, USA
    J Biol Chem 281:26391-9. 2006
    ..We conclude that microtubules and actin filaments restrict cAMP formation by regulating the localization and interaction of GPCR-G(s)-AC in lipid rafts/caveolae...
  91. doi beta-Dystroglycan modulates the interplay between actin and microtubules in human-adhered platelets
    Doris Cerecedo
    Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional IPN, Mexico
    Br J Haematol 141:517-28. 2008
    ..Altogether our data are indicative of a dynamic co-participation of actin filaments and microtubules in modulating focal contacts to achieve platelet function...
  92. doi Arf6 guanine-nucleotide exchange factor, cytohesin-2, interacts with actinin-1 to regulate neurite extension
    Tomohiro Torii
    Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya, Tokyo 157 8535, Japan
    Cell Signal 24:1872-82. 2012
    ....
  93. pmc Identification of gene pathways implicated in Alzheimer's disease using longitudinal imaging phenotypes with sparse regression
    Matt Silver
    Statistics Section, Department of Mathematics, Imperial College London, UK
    Neuroimage 63:1681-94. 2012
    ..Other high ranking previously validated AD endophenotype-related genes include CR1, TOMM40 and APOE...
  94. pmc Intronic parent-of-origin dependent differential methylation at the Actn1 gene is conserved in rodents but is not associated with imprinted expression
    John D Calaway
    Curriculum in Genetics and Molecular Biology, Department of Genetics, Lineberger Comprehensive Cancer Center and Carolina Center for Genome Sciences of the University of North Carolina UNC, Chapel Hill, NC, USA
    PLoS ONE 7:e48936. 2012
    ....
  95. pmc Competition between α-actinin and Ca²⁺-calmodulin controls surface retention of the L-type Ca²⁺ channel Ca(V)1.2
    Duane D Hall
    Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USA
    Neuron 78:483-97. 2013
    ..2 at the plasma membrane and that its displacement by Ca²⁺-calmodulin triggers Ca²⁺-induced endocytosis of Ca(V)1.2, thus providing an important negative feedback mechanism for Ca²⁺ influx...
  96. doi Proteomics screen to reveal molecular changes mediated by C722G missense mutation in CHRM2 gene
    Dongyan Hou
    Heart Failure Center, Department of Cardiology, Capital Medical University, Chao Yang Hospital, Beijing, China
    J Proteomics 89:39-50. 2013
    ..This novel study demonstrated for the first time a full-scale screening of the proteomics research by CHRM2-C722G mutation and profiled 102 changed proteins, of which, eight might be critical in cardiac dysfunction for future mapping...
  97. ncbi Alpha-actinin and vinculin in human neutrophils: reorganization during adhesion and relation to the actin network
    B Yürüker
    Department of Pathology, University of Bern, Switzerland
    J Cell Sci 101:403-14. 1992
    ..Our results suggest a specialized function of vinculin in adhesion sites of human neutrophils, whereas alpha-actinin may structure the actin network in retraction fibers and in less adherent pseudopods...
  98. ncbi Transfection of chicken skeletal muscle alpha-actinin cDNA into nonmuscle and myogenic cells: dimerization is not essential for alpha-actinin to bind to microfilaments
    Y Tokuue
    Department of Biology, Chiba University, Japan
    Exp Cell Res 197:158-67. 1991
    ..Thus, dimer formation is not essential for alpha-actinin molecules to bind to microfilaments...
  99. ncbi Non-viral cellular substrates for human immunodeficiency virus type 1 protease
    R L Shoeman
    Max Planck Institut für Zellbiologie, Ladenburg Heidelberg, Germany
    FEBS Lett 278:199-203. 1991
    ....
  100. ncbi Expression of human alpha-actinin in human hepatocellular carcinoma
    M Nishiyama
    Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Charlestown 02129
    Cancer Res 50:6291-4. 1990
    ..We conclude that the alpha-actinin gene is highly expressed when hepatocytes have assumed the malignant phenotype...
  101. ncbi Identification of the cytoskeletal protein alpha-actinin as a platelet thrombospondin-binding protein
    V Dubernard
    Unité INSERM 353, Hopital Saint Louis, Paris, France
    FEBS Lett 364:109-14. 1995
    ..We further demonstrated that [125I]TSP interacts with alpha-actinin in a specific manner and with a high affinity (Kd = 6.6 nM) in a solid-phase binding assay...

Research Grants2

  1. Richard A Firtel; Fiscal Year: 2015
    ..Roco2 acts upstream of the F-actin cross- linking protein filamin and both are required for pseudopod extension. We will examine the mechanism by which this pathway is regulated and how it controls chemotaxis. ..
  2. Role of Clic in Epithelial Morphogenesis
    Mark Berryman; Fiscal Year: 2008
    ..The fruit fly will be used as a model system to understand how Clic works with other genes and proteins known to be important for cell shape and movement during embryonic development. [unreadable] [unreadable] [unreadable]..