Syt1

Summary

Gene Symbol: Syt1
Description: Synaptotagmin 1
Alias: CG3139, D. Syt I, DSYT, DSYT2, DSypt, DSyt, DSyt1, Dmel\CG3139, Droso1, Dsyt2, STY1, SYT, SYT I, SYT1, Syt, Syt 1, Syt I, Syt-1, SytI, dSYT, dSyt1, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, l(2)k05909, syt, syt 1, syt I, syt-1, syt1, sytI, CG3139 gene product from transcript CG3139-RI, CG3139-PA, CG3139-PB, CG3139-PC, CG3139-PE, CG3139-PF, CG3139-PG, CG3139-PH, CG3139-PI, Synaptotagmin-1, Synaptotagmin-I, Synaptotagmin1, SynaptotagminI, Syt1-PA, Syt1-PB, Syt1-PC, Syt1-PE, Syt1-PF, Syt1-PG, Syt1-PH, Syt1-PI, synaptoptagmin 1, synaptotagmin, synaptotagmin 1, synaptotagmin I
Species: fruit fly

Top Publications

  1. ncbi Synaptotagmins I and IV promote transmitter release independently of Ca(2+) binding in the C(2)A domain
    Iain M Robinson
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Nature 418:336-40. 2002
  2. ncbi Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
    Giuseppa Pennetta
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Neuron 35:291-306. 2002
  3. pmc A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission
    Ilya Vilinsky
    Department of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853, USA
    Genetics 162:259-71. 2002
  4. ncbi Living synaptic vesicle marker: synaptotagmin-GFP
    Yong Q Zhang
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235 1634, USA
    Genesis 34:142-5. 2002
  5. ncbi Synaptotagmin I functions as a calcium sensor to synchronize neurotransmitter release
    Motojiro Yoshihara
    Picower Center for Learning and Memory, Cambridge, MA, USA
    Neuron 36:897-908. 2002
  6. pmc A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction
    Jan Pielage
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 175:491-503. 2006
  7. pmc A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila melanogaster photoreceptors
    W Ryan Williamson
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 189:885-99. 2010
  8. ncbi The stoned proteins regulate synaptic vesicle recycling in the presynaptic terminal
    T Fergestad
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Neurosci 19:5847-60. 1999
  9. doi Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila
    Julien Séjourné
    Genes and Dynamics of Memory Systems, Neurobiology Unit, CNRS, Ecole Superieure de Physique et de Chimie Industrielles, Paris, France
    Nat Neurosci 14:903-10. 2011
  10. ncbi Characterization of Drosophila fruitless-gal4 transgenes reveals expression in male-specific fruitless neurons and innervation of male reproductive structures
    Jean Christophe Billeter
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, G11 6NU Glasgow, United Kingdom
    J Comp Neurol 475:270-87. 2004

Research Grants

Scientific Experts

Detail Information

Publications153 found, 100 shown here

  1. ncbi Synaptotagmins I and IV promote transmitter release independently of Ca(2+) binding in the C(2)A domain
    Iain M Robinson
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Nature 418:336-40. 2002
    ..The most extensively studied candidate for the Ca(2+)-sensing trigger is synaptotagmin I, whose Ca(2+)-dependent interactions with acidic phospholipids and syntaxin have largely been ascribed to its ..
  2. ncbi Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
    Giuseppa Pennetta
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Neuron 35:291-306. 2002
    ..We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding...
  3. pmc A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission
    Ilya Vilinsky
    Department of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853, USA
    Genetics 162:259-71. 2002
    ..These results suggest that the apparent redundancy between SNAP-25 and SNAP-24 is due to inappropriate genetic substitution...
  4. ncbi Living synaptic vesicle marker: synaptotagmin-GFP
    Yong Q Zhang
    Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235 1634, USA
    Genesis 34:142-5. 2002
  5. ncbi Synaptotagmin I functions as a calcium sensor to synchronize neurotransmitter release
    Motojiro Yoshihara
    Picower Center for Learning and Memory, Cambridge, MA, USA
    Neuron 36:897-908. 2002
    To characterize Ca(2+)-mediated synaptic vesicle fusion, we analyzed Drosophila synaptotagmin I mutants deficient in specific interactions mediated by its two Ca(2+) binding C2 domains...
  6. pmc A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction
    Jan Pielage
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 175:491-503. 2006
    ..We propose a model in which a postsynaptic Spectrin-actin lattice acts as an organizing scaffold upon which pre- and postsynaptic development are arranged...
  7. pmc A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila melanogaster photoreceptors
    W Ryan Williamson
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 189:885-99. 2010
    ..We propose that V100 exerts a temporally integrated dual function that increases neuronal degradative capacity...
  8. ncbi The stoned proteins regulate synaptic vesicle recycling in the presynaptic terminal
    T Fergestad
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Neurosci 19:5847-60. 1999
    ..Mutant terminals also display a specific mislocalization of the synaptic vesicle protein synaptotagmin. These results suggest that the stoned proteins are essential for the recycling of synaptic vesicle membrane ..
  9. doi Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila
    Julien Séjourné
    Genes and Dynamics of Memory Systems, Neurobiology Unit, CNRS, Ecole Superieure de Physique et de Chimie Industrielles, Paris, France
    Nat Neurosci 14:903-10. 2011
    ..As the lateral horn has been implicated in innate responses to repellent odorants, we propose that MB-V2 neurons recruit the olfactory pathway involved in innate odor avoidance during memory retrieval...
  10. ncbi Characterization of Drosophila fruitless-gal4 transgenes reveals expression in male-specific fruitless neurons and innervation of male reproductive structures
    Jean Christophe Billeter
    Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, G11 6NU Glasgow, United Kingdom
    J Comp Neurol 475:270-87. 2004
    ....
  11. ncbi Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size
    Kira E Poskanzer
    Department of Biochemistry and Biophysics, University of California, San Francisco, Rock Hall, 1550 Fourth Street, Box 2822, San Francisco, California 94158, USA
    Neuron 50:49-62. 2006
    It has been demonstrated that synapses lacking functional synaptotagmin I (Syt I) have a decreased rate of synaptic vesicle endocytosis. Beyond this, the function of Syt I during endocytosis remains undefined...
  12. ncbi The products of the Drosophila stoned locus interact with synaptic vesicles via synaptotagmin
    A M Phillips
    Department of Genetics, The University of Melbourne, Victoria, Australia 3010
    J Neurosci 20:8254-61. 2000
    ..colocalization of the stoned proteins at the neuromuscular junction with the integral synaptic vesicle protein synaptotagmin (SYT). We show here that stoned interacts genetically with synaptotagmin to produce a lethal phenotype...
  13. pmc Functional dissection of a eukaryotic dicistronic gene: transgenic stonedB, but not stonedA, restores normal synaptic properties to Drosophila stoned mutants
    Patricia S Estes
    Department of Molecular and Cellular Biology and ARL Division of Neurobiology, University of Arizona, Tucson, Arizona 85721
    Genetics 165:185-96. 2003
    ..deficits of stoned mutants, including those in organismal viability, evoked transmitter release, and synaptotagmin retrieval from the plasma membrane...
  14. ncbi Disruption of axonal transport by loss of huntingtin or expression of pathogenic polyQ proteins in Drosophila
    Shermali Gunawardena
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 40:25-40. 2003
    ..Thus, disruption of axonal transport by pathogenic polyQ proteins could contribute to early neuropathology in Huntington's and other polyQ expansion diseases...
  15. pmc Stoned B mediates sorting of integral synaptic vesicle proteins
    R Mohrmann
    Department of Biological Sciences, Vanderbilt University, 1210 Medical Research Building III, Nashville, TN 37235 1634, USA
    Neuroscience 153:1048-63. 2008
    ..In support of such role of STNB, synaptic levels of the vesicular glutamate transporter (vGLUT) and synaptotagmin-1 are strongly reduced with diminishing STNB function, while other synaptic proteins are largely unaffected...
  16. doi The Drosophila epsin 1 is required for ubiquitin-dependent synaptic growth and function but not for synaptic vesicle recycling
    Hong Bao
    Department of Zoology, University of Oklahoma, Norman, OK 73019, USA
    Traffic 9:2190-205. 2008
    ..These observations demonstrate that Lqf plays distinct roles in two ubiquitin pathways to regulate structural and functional plasticity of the synapse...
  17. pmc Eight different types of dopaminergic neurons innervate the Drosophila mushroom body neuropil: anatomical and physiological heterogeneity
    Zhengmei Mao
    Department of Molecular and Cellular Biology, Baylor College of Medicine Houston, TX, USA
    Front Neural Circuits 3:5. 2009
    ..Differences in functional response properties of these neurons suggest that they are involved in different behavioral processes...
  18. ncbi Highwire regulates synaptic growth in Drosophila
    H I Wan
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 26:313-29. 2000
    ..Within presynaptic terminals, HIW is localized to the periactive zone surrounding active zones; Fasciclin II (Fas II), which also controls synaptic growth, is found at the same location...
  19. pmc The color-vision circuit in the medulla of Drosophila
    Javier Morante
    Center for Developmental Genetics, Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA
    Curr Biol 18:553-65. 2008
    ..This paper analyzes the neural network underlying color vision into the medulla...
  20. ncbi A product of the Drosophila stoned locus regulates neurotransmitter release
    D T Stimson
    Arizona Research Laboratories Division of Neurobiology and Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    J Neurosci 18:9638-49. 1998
    ..Remarkably, almost all phenotypes of stoned mutants are similar to those previously described for mutants of synaptotagmin, a protein postulated to regulate both exocytosis and the recycling of synaptic vesicles...
  21. ncbi Drosophila synaptotagmin I null mutants survive to early adulthood
    C A Loewen
    Department of Anatomy and Neurobiology, Program in Molecular, Cellular, and Integrative Neuroscience, Colorado State University, Fort Collins, Colorado 80523 1670, USA
    Genesis 31:30-6. 2001
    ..have been limited by the early (embryonic or first instar) lethality previously reported for Drosophila synaptotagmin I null (syt(null)) mutants...
  22. ncbi Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix
    D Parnas
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Neuron 32:415-24. 2001
    ..Many, but not all, dpix defects are mediated through dPak, a member of the family of Cdc42/Rac1-activated kinases. Thus, a Rho-type GEF and Rho-type effector kinase regulate postsynaptic structure...
  23. ncbi Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function
    Y Q Zhang
    Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    Cell 107:591-603. 2001
    ..dfxr futsch double mutants restore normal synaptic structure and function. We propose that dFXR acts as a translational repressor of Futsch to regulate microtubule-dependent synaptic growth and function...
  24. ncbi Nervous wreck, an SH3 adaptor protein that interacts with Wsp, regulates synaptic growth in Drosophila
    Ian P Coyle
    Laboratory of Genetics, University of Wisconsin, Madison, WI 53706, USA
    Neuron 41:521-34. 2004
    ..We propose that Nwk controls synapse morphology by regulating actin dynamics downstream of growth signals in presynaptic terminals...
  25. pmc Calcium dependence of neurotransmitter release and rate of spontaneous vesicle fusions are altered in Drosophila synaptotagmin mutants
    J T Littleton
    Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030
    Proc Natl Acad Sci U S A 91:10888-92. 1994
    ..b>Synaptotagmin is an integral membrane protein of synaptic vesicles that contains two copies of a domain known to be involved ..
  26. pmc A genetic method for generating Drosophila eyes composed exclusively of mitotic clones of a single genotype
    R S Stowers
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 152:1631-9. 1999
    ..We illustrate the utility of the method by applying it to lethal mutations in the synaptic transmission genes synaptotagmin and syntaxin.
  27. ncbi Structural and functional conservation of synaptotagmin (p65) in Drosophila and humans
    M S Perin
    Howard Hughes Medical Institute, Dallas, Texas
    J Biol Chem 266:615-22. 1991
    b>Synaptotagmin (p65) is an abundant synaptic vesicle protein that contains two copies of a sequence that is homologous to the regulatory region of protein kinase C...
  28. ncbi Synaptic transmission persists in synaptotagmin mutants of Drosophila
    A DiAntonio
    Department of Molecular and Cellular Physiology, Stanford University Medical Center, California 94305 5426
    Cell 73:1281-90. 1993
    b>Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles...
  29. ncbi synaptotagmin mutants reveal essential functions for the C2B domain in Ca2+-triggered fusion and recycling of synaptic vesicles in vivo
    J T Littleton
    Department of Physiology and Entomology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Neurosci 21:1421-33. 2001
    b>Synaptotagmin has been proposed to function as a Ca(2+) sensor that regulates synaptic vesicle exocytosis, whereas the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex is thought to form the core of a ..
  30. ncbi Drosophila stoned proteins regulate the rate and fidelity of synaptic vesicle internalization
    D T Stimson
    Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, Arizona 85721, USA
    J Neurosci 21:3034-44. 2001
    ..These observations are consistent with a model in which stonedA and stonedB act to facilitate the internalization of synaptic vesicle components from the plasma membrane...
  31. ncbi Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I
    Kurt W Marek
    Department of Biochemistry, University of California, San Francisco, 94143, USA
    Neuron 36:805-13. 2002
    ..fluorescein derivative 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH) was engineered into synaptotagmin I (Syt I4C)...
  32. pmc Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function
    Nina Tang Sherwood
    Broad Center, Division of Biology, California Institute of Technology Pasadena, California, USA
    PLoS Biol 2:e429. 2004
    ....
  33. ncbi Drosophila homologue of Eps15 is essential for synaptic vesicle recycling
    Amitabha Majumdar
    Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba Mumbai 400005, India
    Exp Cell Res 312:2288-98. 2006
    ..Genetic and biochemical studies of interactions with components of the endocytic machinery suggest that Eps15 has an important role in synaptic vesicle recycling and regulates recruitment of alpha-Adaptin...
  34. pmc Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction
    Sara Seabrooke
    Department of Biology, University of Toronto, Mississauga, ON L5L 1C6, Canada
    BMC Neurosci 11:37. 2010
    ..The actin cytoskeleton has been implicated but exactly how actin or actin-binding proteins participate in vesicle movement is not clear...
  35. pmc Differential regulation of synchronous versus asynchronous neurotransmitter release by the C2 domains of synaptotagmin 1
    Motojiro Yoshihara
    Picower Institute for Learning and Memory, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:14869-74. 2010
    ..Mutations in the synaptic vesicle Ca(2+) sensor Synaptotagmin 1 (Syt 1) reduce synchronous neurotransmission while enhancing the slower asynchronous phase of release...
  36. ncbi Wiring by fly: the neuromuscular system of the Drosophila embryo
    M Bate
    Department of Zoology, University of Cambridge, England
    Neuron 15:513-25. 1995
  37. ncbi Expression of synaptotagmin in Drosophila reveals transport and localization of synaptic vesicles to the synapse
    J T Littleton
    Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030
    Development 118:1077-88. 1993
    b>Synaptotagmin is a synaptic vesicle-specific integral membrane protein that has been suggested to play a key role in synaptic vesicle docking and fusion...
  38. ncbi Role of neural activity during synaptogenesis in Drosophila
    J Jarecki
    Department of Genetics, Yale University, New Haven, Connecticut 06511, USA
    J Neurosci 15:8177-90. 1995
    ..Our experiments suggest that neural activity may function during development by preventing inappropriate connections and thereby maintaining the precise connectivity achieved during nerve outgrowth and target selection...
  39. ncbi Functional expression of rat synapse-associated proteins SAP97 and SAP102 in Drosophila dlg-1 mutants: effects on tumor suppression and synaptic bouton structure
    U Thomas
    Federal Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, Magdeburg, Germany
    Mech Dev 62:161-74. 1997
    ....
  40. ncbi Genetic analysis of the mechanisms controlling target selection: target-derived Fasciclin II regulates the pattern of synapse formation
    G W Davis
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 19:561-73. 1997
    ..Changing the relative levels of Fas II on neighboring muscles leads to dramatic shifts in target selection...
  41. ncbi Absence of PS integrins or laminin A affects extracellular adhesion, but not intracellular assembly, of hemiadherens and neuromuscular junctions in Drosophila embryos
    A Prokop
    Department of Zoology, Downing Street, Cambridge, CB2 3EJ, United Kingdom
    Dev Biol 196:58-76. 1998
    ..We suggest that neuromuscular contact in part requires basement membrane adhesion to the general muscle surface, and this form of adhesion is completely abolished in the absence of laminin A...
  42. ncbi Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila
    K Zito
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 22:719-29. 1999
    ..Some new boutons exhibit a lower level of Fasciclin II, suggesting that the levels of this synaptic cell adhesion molecule vary locally during synaptic growth...
  43. pmc Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport
    M Martin
    Department of Biology, Indiana University, Bloomington, Indiana 47405 6801, USA
    Mol Biol Cell 10:3717-28. 1999
    ..The shared bidirectional disruption phenotypes and the dominant genetic interactions demonstrate that cytoplasmic dynein, the dynactin complex, and conventional kinesin are interdependent in fast axonal transport...
  44. ncbi Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin
    Z Nie
    Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6974, USA
    J Neurosci 19:10270-9. 1999
    ....
  45. ncbi Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release
    D E Featherstone
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
    J Neurosci 21:4215-24. 2001
    ....
  46. ncbi wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila
    Hermann Aberle
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Neuron 33:545-58. 2002
    ..The NMJ phenotype is specifically rescued by transgenic expression of Wit only in motoneurons. Thus, Wit appears to function as a presynaptic receptor that regulates synaptic size at the Drosophila NMJ...
  47. ncbi Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis
    Nancy Kaufmann
    Department of Cell Biology, Program in Neuroscience and DFCI Harvard Cancer Center, Harvard Medical School, Boston, MA 02115, USA
    Neuron 34:27-38. 2002
    ..These data suggest a model where Dliprin-alpha and Dlar cooperate to regulate the formation and/or maintenance of a network of presynaptic proteins...
  48. ncbi Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release
    Patrik Verstreken
    Program in Developmental Biology, Houston, TX, USA
    Cell 109:101-12. 2002
    ..We propose that kiss-and-run maintains neurotransmission at active zones of the larval NMJ in endophilin animals...
  49. ncbi Compartmentalization of central neurons in Drosophila: a new strategy of mosaic analysis reveals localization of presynaptic sites to specific segments of neurites
    Robert Lohr
    Institute of Genetics, University of Mainz, D 55128 Mainz, Germany
    J Neurosci 22:10357-67. 2002
    ..This suggests that synaptogenic mechanisms in the CNS might differ from those at neuromuscular junctions...
  50. ncbi Axonal transport of mitochondria to synapses depends on milton, a novel Drosophila protein
    R Steven Stowers
    Department of Molecular and Cell Biology, University of California, Berkeley, Life Sciences Addition, Room 548, Berkeley, CA 94720, USA
    Neuron 36:1063-77. 2002
    ..Transfected into HEK293T cells, Milton induces a redistribution of mitochondria within the cell. We propose that Milton is a mitochondria-associated protein required for kinesin-mediated transport of mitochondria to nerve terminals...
  51. ncbi The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction
    Brian D McCabe
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Neuron 39:241-54. 2003
    ..These experiments reveal that a muscle-derived BMP retrograde signal participates in coordinating neuromuscular synapse development and growth...
  52. ncbi Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo
    Kira E Poskanzer
    Department of Biochemistry and Biophysics, Program in Neuroscience, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nature 426:559-63. 2003
    Neurotransmission requires a balance of synaptic vesicle exocytosis and endocytosis. Synaptotagmin I (Syt I) is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis...
  53. ncbi The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function
    Nick Trotta
    Department of Biological Sciences, Vanderbilt Kennedy Center for Research on Human Development, Vanderbilt University, Nashville, TN 37235, USA
    Curr Biol 14:1135-47. 2004
    ..Previous work in cell culture has proposed a role for Spastin in regulating microtubules...
  54. pmc Ceramidase regulates synaptic vesicle exocytosis and trafficking
    Jeffrey Rohrbough
    Department of Biological Sciences, Vanderbilt Kennedy Center, Vanderbilt Brain Institute, Vanderbilt University, Nashville, Tennessee 37235 1634, USA
    J Neurosci 24:7789-803. 2004
    ..These results indicate that SLAB ceramidase function controls presynaptic terminal sphingolipid composition to regulate vesicle fusion and trafficking, and thus the strength and reliability of synaptic transmission...
  55. ncbi Direct observation demonstrates that Liprin-alpha is required for trafficking of synaptic vesicles
    Kyle E Miller
    Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 15:684-9. 2005
    ..Here, at the light and ultrastructural levels, we discover aberrant accumulations of synaptic vesicle markers (Synaptotagmin and Synaptobrevin-GFP) and clear-core vesicles along Drosophila Liprin-alpha mutant axons...
  56. pmc APLIP1, a kinesin binding JIP-1/JNK scaffold protein, influences the axonal transport of both vesicles and mitochondria in Drosophila
    Dai Horiuchi
    Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
    Curr Biol 15:2137-41. 2005
    ..However, it is also worth considering that APLIP1 and its associated JNK signaling proteins could serve as an important signaling module for regulating transport by the two opposing motors...
  57. pmc Drosophila melanogaster Scramblases modulate synaptic transmission
    Usha Acharya
    Laboratory of Protein Dynamics and Signaling, National Cancer Institute Frederick, Frederick, MD 21702, USA
    J Cell Biol 173:69-82. 2006
    ..The lack of phenotypes related to failure of scrambling and the neurophysiological analysis lead us to propose that Scramblases play a modulatory role in the process of neurotransmission...
  58. ncbi Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila
    Xiankun Zeng
    Department of Genetics and Developmental Biology, Genetics Research Center, Southeast University Medical School, Ministry of Education, 87 Dingjiaqiao Road, Nanjing 210009, Jiangsu Province, China
    FEBS Lett 581:2509-16. 2007
    ..The synapse number is decreased in central nervous system in Neurexin-1 null mutants. In addition, Neurexin-1 null mutants exhibit associative learning defect in larvae...
  59. ncbi A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport
    Eunju Pack-Chung
    Program in Neurobiology, Children s Hospital, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Neurosci 10:980-9. 2007
    ..Our data thus indicate that Imac transports components required for synaptic maturation and provide insight into presynaptic maturation as a process that can be differentiated from axon outgrowth and targeting...
  60. pmc UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly
    Hirofumi Toda
    Division of Neurosciences, Beckman Research Institute of the City of Hope, California 91010, USA
    Genes Dev 22:3292-307. 2008
    ..UNC-51 phosphorylates UNC-76 on Ser(143), and the phosphorylated UNC-76 binds Synaptotagmin-1, a synaptic vesicle protein, suggesting that motor-cargo interactions are regulated in a phosphorylation-..
  61. ncbi Syntaxin and synaptobrevin function downstream of vesicle docking in Drosophila
    K Broadie
    Department of Zoology, University of Cambridge, England
    Neuron 15:663-73. 1995
    ..We conclude that the SNARE hypothesis cannot fully explain the role of these proteins in synaptic transmission. Instead, both proteins play distinct roles downstream of docking...
  62. ncbi Identification and characterization of Drosophila genes for synaptic vesicle proteins
    A DiAntonio
    Department of Molecular and Cellular Physiology, Stanford University Medical Center, California 94305 5426
    J Neurosci 13:4924-35. 1993
    ..This investigation describes Drosophila homologs of vamp, synaptotagmin, and rab3 that are expressed in a pattern consistent with a function in Drosophila neurotransmission...
  63. ncbi Traffic of dynamin within individual Drosophila synaptic boutons relative to compartment-specific markers
    P S Estes
    Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA
    J Neurosci 16:5443-56. 1996
    ..We suggest that these dynamin-rich domains may mark the active zones for synaptic vesicle endocytosis first described at the frog neuromuscular junction...
  64. ncbi Drosophila genetics and the functions of synaptic proteins
    K Parfitt
    Department of Molecular and Cellular Biology, Stanford University, California 94035, USA
    Cold Spring Harb Symp Quant Biol 60:371-7. 1995
  65. ncbi Presynaptic development at the Drosophila neuromuscular junction: assembly and localization of presynaptic active zones
    A Prokop
    Department of Zoology, University of Cambridge, United Kingdom
    Neuron 17:617-26. 1996
    ..Thus, the process of synapse formation can be genetically separated from the process of target recognition, revealing that localization of presynaptic active zones requires mef2-dependent muscle differentiation...
  66. ncbi Genetic dissection of structural and functional components of synaptic plasticity. II. Fasciclin II controls presynaptic structural plasticity
    C M Schuster
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 17:655-67. 1996
    ....
  67. ncbi Genetic dissection of structural and functional components of synaptic plasticity. III. CREB is necessary for presynaptic functional plasticity
    G W Davis
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA
    Neuron 17:669-79. 1996
    ..Expression of dCREB2-a in a FasII mutant background genetically reconstitutes this cAMP-dependent plasticity. Thus, cAMP initiates parallel changes in CREB and Fas II to achieve long-term synaptic enhancement...
  68. ncbi Role of Drosophila alpha-adaptin in presynaptic vesicle recycling
    M Gonzalez-Gaitan
    Max Planck Institut fur biophysikalische Chemie, Abt Molekulare Entwicklungsbiologie, Gottingen, Germany
    Cell 88:767-76. 1997
    ..Our results suggest an alpha-adaptin-dependent control of the vesicle cycle that maintains the balance between the amount of vesicle- and surface-associated membranes...
  69. ncbi Transition from growth cone to functional motor nerve terminal in Drosophila embryos
    M Yoshihara
    Gunma University School of Medicine, Maebashi 371, Japan
    J Neurosci 17:8408-26. 1997
    ..b>Synaptotagmin immunoreactive clusters form concurrently...
  70. ncbi A peritracheal neuropeptide system in insects: release of myomodulin-like peptides at ecdysis
    M A O'Brien
    Department of Anatomy, Washington University School of Medicine, Saint Louis, MO 63110, USA
    J Exp Biol 201:193-209. 1998
    ..These data suggest that peritracheal cells release MM-like peptides as part of a conserved feature of the endocrine regulation of insect ecdysis...
  71. pmc Kinesin light chains are essential for axonal transport in Drosophila
    J G Gindhart
    Howard Hughes Medical Institute, Division of Cellular and Molecular Medicine, Department of Pharmacology, University of California, San Diego, La Jolla, California 92093 0683, USA
    J Cell Biol 141:443-54. 1998
    ..Cell 64:1093-1102; Hurd, D.D., and W.M. Saxton. 1996. Genetics 144: 1075-1085) mutant phenotypes indicates that KLC is essential for kinesin function, perhaps by tethering KHC to intracellular cargos or by activating the kinesin motor...
  72. ncbi Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly
    J T Littleton
    Laboratory of Genetics, University of Wisconsin, Madison 53706, USA
    Neuron 21:401-13. 1998
    ..These results provide in vivo evidence that cycles of assembly and disassembly of SNARE complexes drive membrane trafficking at synapses...
  73. ncbi Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosis
    B Zhang
    Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Neuron 21:1465-75. 1998
    ..These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process...
  74. ncbi Neuronal overexpression of APPL, the Drosophila homologue of the amyloid precursor protein (APP), disrupts axonal transport
    L Torroja
    Department of Biology, Center for Complex Systems, MS 008, Brandeis University Waltham, Massachusetts, 02454, USA
    Curr Biol 9:489-92. 1999
    ....
  75. ncbi Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo
    M N Wu
    Department of Cell Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Neuron 23:593-605. 1999
    ..we identify and characterize a syntaxin Ca2+ effector domain, which may spatially organize the Ca2+ channel, cysteine string protein, and synaptotagmin for effective excitation-secretion coupling in the presynaptic terminal.
  76. ncbi Drosophila Futsch regulates synaptic microtubule organization and is necessary for synaptic growth
    J Roos
    Dept Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Neuron 26:371-82. 2000
    ..These data suggest a common microtubule-based growth mechanism at the synapse and growth cone...
  77. ncbi Drosophila endosomal proteins hook and deep orange regulate synapse size but not synaptic vesicle recycling
    R Narayanan
    Department of Molecular and Cellular Biology and ARL, Division of Neurobiology, University of Arizona, Life Sciences South, Box 210106, Tucson, Arizona 85721, USA
    J Neurobiol 45:105-19. 2000
    ..Our observations suggest a model in which Hook and Dor function in later stages of endocytosis is essential for regulating synaptic plasma membrane composition but not synaptic vesicle recycling...
  78. ncbi Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis
    T Fergestad
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
    J Neurosci 21:1218-27. 2001
    ..We have documented previously a highly specific synaptic mislocalization and degradation of Synaptotagmin I in stoned mutants...
  79. ncbi Mutations in the second C2 domain of synaptotagmin disrupt synaptic transmission at Drosophila neuromuscular junctions
    J M Mackler
    Molecular, Cellular, and Integrative Neuroscience Program, Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Comp Neurol 436:4-16. 2001
    ..We have focused on the second C2 domain (C2B) of synaptotagmin I, in particular, on a series of conserved lysine residues on beta-strand 4 of C2B...
  80. ncbi Disruption of axonal transport and neuronal viability by amyloid precursor protein mutations in Drosophila
    S Gunawardena
    Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093 0683, USA
    Neuron 32:389-401. 2001
    ..We discuss the possibility that axonal transport disruption may play a role in the neurodegenerative pathology of Alzheimer's disease...
  81. ncbi The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo
    J M Mackler
    Department of Anatomy and Neurobiology, Program in Molecular, Cellular, and Integrative Neuroscience, Colorado State University, Fort Collins, Colorado 80523, USA
    Nature 418:340-4. 2002
    b>Synaptotagmin is a synaptic vesicle protein that is postulated to be the Ca(2+) sensor for fast, evoked neurotransmitter release...
  82. ncbi Mutations in the exocyst component Sec5 disrupt neuronal membrane traffic, but neurotransmitter release persists
    Mala Murthy
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    Neuron 37:433-47. 2003
    ..In this regard, sec5 differs from the homologs of other yeast exocytosis genes that are required for both neuronal trafficking pathways...
  83. ncbi Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites
    Matthias Landgraf
    Department of Zoology, Downing Street, CB2 3EJ, Cambridge, UK
    Dev Biol 260:207-25. 2003
    ..Using the Fasciclin2-based mapping procedure as a standard (e.g., in a common database) would facilitate studies on the functional architecture of the neuropile and the identification of candiate circuit elements...
  84. pmc The kinesin-associated protein UNC-76 is required for axonal transport in the Drosophila nervous system
    Joseph G Gindhart
    Biology Department, University of Massachusetts, Boston, Massachusetts 02125, USA
    Mol Biol Cell 14:3356-65. 2003
    ..Given the interaction of FEZ1, the mammalian homolog of UNC-76, with protein kinase Czeta, and the role of FEZ1 in axon outgrowth, we propose that UNC-76 helps integrate kinesin activity in response to transport requirements in axons...
  85. ncbi Morphology and molecular organization of the adult neuromuscular junction of Drosophila
    Patricia K Rivlin
    Department of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853, USA
    J Comp Neurol 468:596-613. 2004
    ..Furthermore, these results demonstrate that there are major differences in the molecular organization of the adult and larval NMJs...
  86. ncbi Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis
    Tong Wey Koh
    Program in Developmental Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Neuron 43:193-205. 2004
    ..Our data suggest that Dap160, like dynamin, is involved in synaptic vesicle retrieval at active and periactive zones...
  87. pmc Synaptotagmins are trafficked to distinct subcellular domains including the postsynaptic compartment
    Bill Adolfsen
    The Picower Center for Learning and Memory, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    J Cell Biol 166:249-60. 2004
    The synaptotagmin family has been implicated in calcium-dependent neurotransmitter release, although Synaptotagmin 1 is the only isoform demonstrated to control synaptic vesicle fusion...
  88. ncbi AP180 maintains the distribution of synaptic and vesicle proteins in the nerve terminal and indirectly regulates the efficacy of Ca2+-triggered exocytosis
    Hong Bao
    Section of Neurobiology, Institute for Neuroscience, 1 University Station, The University of Texas at Austin, Austin, Texas 78712, USA
    J Neurophysiol 94:1888-903. 2005
    ..Consistent with this hypothesis, three vesicle proteins critical for calcium-mediated exocytosis, synaptotagmin I, cysteine-string protein, and neuronal synaptobrevin, are all mislocalized to the extrasynaptic axonal ..
  89. pmc The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila
    P Robin Hiesinger
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 121:607-20. 2005
    ..Our data suggest that Vha100-1 functions downstream of SNAREs in synaptic vesicle fusion...
  90. ncbi Are dendrites in Drosophila homologous to vertebrate dendrites?
    Natalia Sánchez-Soriano
    Faculty of Life Sciences, WTCCMR, The University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Dev Biol 288:126-38. 2005
    ..Implications of these findings for studies of dendrite development, neuronal polarity, transport and evolution are discussed...
  91. ncbi Fruitless specifies sexually dimorphic neural circuitry in the Drosophila brain
    Ken ichi Kimura
    Laboratory of Biology, Iwamizawa Campus, Hokkaido University of Education, Iwamizawa 068 8642, Japan
    Nature 438:229-33. 2005
    ..Thus, Fru expression can produce a male-specific neural circuit, probably used during heterosexual courtship, by preventing cell death in identifiable neurons...
  92. ncbi Altered synaptic development and active zone spacing in endocytosis mutants
    Dion K Dickman
    Division of Neuroscience, Children s Hospital, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 16:591-8. 2006
    ..Mutants in endophilin, synaptojanin, dynamin, AP180, and synaptotagmin all show increases in supernumerary bouton structures...
  93. pmc Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent
    Elizabeth E Glater
    Neurobiology Program, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 173:545-57. 2006
    ..Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain-independent, anterograde transport of mitochondria...
  94. ncbi Highwire restrains synaptic growth by attenuating a MAP kinase signal
    Catherine A Collins
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Washington University, St Louis, Missouri 63110, USA
    Neuron 51:57-69. 2006
    ..In addition to controlling synaptic growth, Highwire promotes synaptic function through a separate pathway that does not require wallenda...
  95. pmc Synaptic vesicle mobility and presynaptic F-actin are disrupted in a N-ethylmaleimide-sensitive factor allele of Drosophila
    Paula Nunes
    Department of Biology and Computer Science, University of Toronto, Mississauga, Ontario, Canada
    Mol Biol Cell 17:4709-19. 2006
    ..Our data are consistent with a model in which actin filaments promote vesicle mobility and suggest that NSF participates in establishing or maintaining this population of actin...
  96. ncbi Modular neuropile organization in the Drosophila larval brain facilitates identification and mapping of central neurons
    Balaji G Iyengar
    Department of Physiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Comp Neurol 499:583-602. 2006
    ..Thus, in the Drosophila larval brain, modular neuropiles can be used as a framework for systematically identifying, mapping, and classifying interneurons; understanding their roles in behavior can then be pursued further...
  97. ncbi Nerve-evoked synchronous release and high K+ -induced quantal events are regulated separately by synaptotagmin I at Drosophila neuromuscular junctions
    Takuya Tamura
    Institute for Molecular and Cellular Neuroscience, Gunma University, Maebashi, Japan
    J Neurophysiol 97:540-9. 2007
    The distal Ca(2+)-binding domain of synaptotagmin I (Syt I), C2B, has two Ca(2+)-binding sites...
  98. pmc Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules
    Xinnan Wang
    Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
    Nat Neurosci 10:177-85. 2007
    ....
  99. ncbi Axonal accumulation of synaptic markers in APP transgenic Drosophila depends on the NPTY motif and is paralleled by defects in synaptic plasticity
    Patricia Rusu
    Zentrum für Molekulare Biologie ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Eur J Neurosci 25:1079-86. 2007
    ....
  100. pmc Genetic modifiers of Drosophila palmitoyl-protein thioesterase 1-induced degeneration
    Haley Buff
    Department of Biology, The College of Charleston, Charleston, South Carolina 29424, USA
    Genetics 176:209-20. 2007
    ..A greater understanding of Ppt1 function in these cellular processes will provide valuable insight into the molecular etiology of the neuronal dysfunction underlying the disease...
  101. ncbi Circadian changes in Drosophila motor terminals
    Kerstin I Mehnert
    Zoology Department, Stockholm University, Stockholm, Sweden
    Dev Neurobiol 67:415-21. 2007
    ..These unexpected results reveal further circadian as well as nonclock related pleiotropic effects for these classic behavioral mutants...

Research Grants5

  1. The Role of Ceramide Kinase in Neurosecretion
    Sandra Bajjalieh; Fiscal Year: 2002
    ..This work will lay the foundation for biochemical and genetic studies of the role of ceramide kianse, its substrate and its product in neurotransmission and how changes in its activity contribute to synaptic plasticity. ..
  2. MOLECULAR ANALYSIS OF THE DROSOPHILA GABA TRANSPORTERS
    Wendi Neckameyer; Fiscal Year: 2001
    ....
  3. Hormonal Factors and Recruitment of Neurons into the Stress Response Circuitry
    Wendi S Neckameyer; Fiscal Year: 2010
    ..This will provide a unique and innovative approach to modeling the development and organization of the stress circuitry in mammals. ..
  4. SYNAPTOTAGMIN/SV2 COMPLEX--ROLE IN NEUROTRANSMISSION
    Sandra Bajjalieh; Fiscal Year: 2001
    ..and her students have identified a protein complex that contains the synaptic vesicle proteins SV2 and synaptotagmin. SV2 is a transporter-like membrane glycoprotein hypothesized to function either as a small molecule ..
  5. FUNCTIONAL ANALYSIS OF SV2 BY TARGETED GENE DISRUPTION
    Sandra Bajjalieh; Fiscal Year: 2009
    ..2. To use exogeneous protein expression in hippocampal neurons to test hypotheses of SV2 function. 3. To test the hypothesis that SV2B plays a crucial role in ribbon synapse maintenance and/or functioning. ..