rho

Summary

Gene Symbol: rho
Description: rhomboid
Alias: CG1004, DMRHO, DMRHOa, DMRHOb, DRORHO, DmRho1, Dmel\CG1004, Dmel\rho, Dmrho, RHO, RHOb, Rho, Rho-1, Rho1, iks, rho-1, rho1, rhom, rhomboid/veinlet, CG1004 gene product from transcript CG1004-RB, CG1004-PA, CG1004-PB, rho-PA, rho-PB, rhomboid 1, rhomboid-1, rhomboid/veinlet, rhomboid1, veinlet
Species: fruit fly

Top Publications

  1. ncbi Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila
    D A Baker
    Cell Signalling Unit, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Nature 411:330-4. 2001
  2. ncbi Multiplex detection of RNA expression in Drosophila embryos
    Dave Kosman
    Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA
    Science 305:846. 2004
  3. pmc RNA polymerase is poised for activation across the genome
    Ginger W Muse
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Nat Genet 39:1507-11. 2007
  4. doi Coordinated sequential action of EGFR and Notch signaling pathways regulates proneural wave progression in the Drosophila optic lobe
    Tetsuo Yasugi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Development 137:3193-203. 2010
  5. ncbi Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster
    L Konrad
    Institut fur Genetik, Johannes Gutenberg Universitat, Mainz, Federal Republic of Germany
    Dev Biol 163:98-111. 1994
  6. ncbi Drosophila rhomboid-1 defines a family of putative intramembrane serine proteases
    S Urban
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 107:173-82. 2001
  7. pmc MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
    Daniel R Marenda
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Development 133:43-51. 2006
  8. pmc EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors
    Huaqi Jiang
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA 98109, USA
    Development 136:483-93. 2009
  9. ncbi The dorsal gradient morphogen regulates stripes of rhomboid expression in the presumptive neuroectoderm of the Drosophila embryo
    Y T Ip
    Biology Department, University of California, San Diego, La Jolla 92093 0322
    Genes Dev 6:1728-39. 1992
  10. ncbi Alternate functions of the single-minded and rhomboid genes in development of the Drosophila ventral neuroectoderm
    H Xiao
    Program for Molecular and Cellular Biology, University of Massachusetts at Amherst 01003, USA
    Mech Dev 58:65-74. 1996

Research Grants

  1. CONTROL OF TGF-BETA SIGNALING IN DROSOPHILA BY BRINKER
    Gerard Campbell; Fiscal Year: 2004
  2. High-Volume Confocal Imaging System
    Stephen Crews; Fiscal Year: 2005
  3. MSM: CR Multiscale analysis of epithelial patterning: m*
    Stanislav Shvartsman; Fiscal Year: 2007
  4. CELL FATE DETERMINATION IN THE MESODERM OF DROSOPHILA
    Mary Baylies; Fiscal Year: 2008
  5. The structural basis for APP cleavability
    Ya Ha; Fiscal Year: 2009
  6. MECHANISMS OF TRANSCRIPTIONAL REPRESSION IN DROSOPHILA
    GERARD L CAMPBELL; Fiscal Year: 2010
  7. Synaptic Glutamate Release in Drosphila
    Aaron DiAntonio; Fiscal Year: 2008
  8. The Role of Presenilin in Gamma-Secretase Activity
    Michael Wolfe; Fiscal Year: 2009
  9. Biology of Serine Proteases and Serpins
    Carl Hashimoto; Fiscal Year: 2010
  10. Molecular Genetics of Midline Glial Development
    Stephen T Crews; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications148 found, 100 shown here

  1. ncbi Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila
    D A Baker
    Cell Signalling Unit, Institute of Reproductive and Developmental Biology, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
    Nature 411:330-4. 2001
    ..Our study indicates that MAPK signalling specificity may depend on proteins that couple specific substrates to the kinase...
  2. ncbi Multiplex detection of RNA expression in Drosophila embryos
    Dave Kosman
    Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA
    Science 305:846. 2004
    ....
  3. pmc RNA polymerase is poised for activation across the genome
    Ginger W Muse
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Nat Genet 39:1507-11. 2007
    ..This finding indicates a role for regulation of polymerase elongation in the transcriptional responses to dynamic environmental and developmental cues...
  4. doi Coordinated sequential action of EGFR and Notch signaling pathways regulates proneural wave progression in the Drosophila optic lobe
    Tetsuo Yasugi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
    Development 137:3193-203. 2010
    ..EGFR activation is regulated by transient expression of Rhomboid (Rho), which is required for the maturation of the EGF ligand Spitz. Rho expression is also regulated by the EGFR signal...
  5. ncbi Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster
    L Konrad
    Institut fur Genetik, Johannes Gutenberg Universitat, Mainz, Federal Republic of Germany
    Dev Biol 163:98-111. 1994
    ..Homologies of the G-S-rich C-terminus of the putative MBN83 protein to human cytokeratins K1, K10, and mouse loricrin were found. The structure and possible function of the wild-type l(3)mbn-1+ gene are discussed...
  6. ncbi Drosophila rhomboid-1 defines a family of putative intramembrane serine proteases
    S Urban
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 107:173-82. 2001
    ..Rhomboid-1 is conserved throughout evolution from archaea to humans, and our results show that a human Rhomboid promotes Spitz cleavage by a similar mechanism. This growth factor activation mechanism may therefore be widespread...
  7. pmc MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
    Daniel R Marenda
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Development 133:43-51. 2006
    ....
  8. pmc EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors
    Huaqi Jiang
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA 98109, USA
    Development 136:483-93. 2009
    ..Two stronger EGFR ligands, Spitz and Keren, are expressed by the AMPs themselves and provide an additional, autocrine mitogenic stimulus to the AMPs during late larval stages...
  9. ncbi The dorsal gradient morphogen regulates stripes of rhomboid expression in the presumptive neuroectoderm of the Drosophila embryo
    Y T Ip
    Biology Department, University of California, San Diego, La Jolla 92093 0322
    Genes Dev 6:1728-39. 1992
    rhomboid (rho) encodes a putative transmembrane receptor that is required for the differentiation of the ventral epidermis...
  10. ncbi Alternate functions of the single-minded and rhomboid genes in development of the Drosophila ventral neuroectoderm
    H Xiao
    Program for Molecular and Cellular Biology, University of Massachusetts at Amherst 01003, USA
    Mech Dev 58:65-74. 1996
    We have investigated the roles of the single-minded (sim) and rhomboid (rho) genes in generating distinct cell fates in the Drosophila embryonic neuroectoderm...
  11. ncbi Notch signalling regulates veinlet expression and establishes boundaries between veins and interveins in the Drosophila wing
    J F de Celis
    Department of Genetics, University of Cambridge, UK
    Development 124:1919-28. 1997
    ..the expression of the Enhancer of split gene m beta is activated and the transcription of the vein-promoting gene veinlet is repressed, thus restricting vein differentiation...
  12. ncbi In situ activation pattern of Drosophila EGF receptor pathway during development
    L Gabay
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 277:1103-6. 1997
    ..This in situ approach can be used to monitor other receptor-triggered pathways in a wide range of organisms...
  13. ncbi Requirement for EGF receptor signalling in neural recruitment during formation of Drosophila chordotonal sense organ clusters
    P Lage
    Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh EH9 3JR, UK
    Curr Biol 7:166-75. 1997
    ..We present evidence that a similar mechanism is required for the clustering of embryonic chordotonal organs...
  14. ncbi Signalling by the Drosophila epidermal growth factor receptor is required for the specification and diversification of embryonic muscle progenitors
    E Buff
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School and Howard Hughes Medical Institute, Boston, MA 02115, USA
    Development 125:2075-86. 1998
    ..Thus, the epidermal growth factor receptor contributes both to muscle progenitor specification and to the diversification of muscle identities...
  15. ncbi The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis
    C Ghiglione
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 96:847-56. 1999
    ..We show that the extracellular and transmembrane domains of Kek1 can inhibit and physically associate with the EGFR, suggesting potential models for this inhibitory mechanism...
  16. pmc zfh-1, the Drosophila homologue of ZEB, is a transcriptional repressor that regulates somatic myogenesis
    A A Postigo
    Division of Molecular Oncology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Cell Biol 19:7255-63. 1999
    ....
  17. ncbi A temporal switch in DER signaling controls the specification and differentiation of veins and interveins in the Drosophila wing
    E Martin-Blanco
    Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK
    Development 126:5739-47. 1999
    ..At this time, rhomboid (rho), in combination with vein, initiates and amplifies the activity of DER in vein cells...
  18. ncbi Regulation of cell number by MAPK-dependent control of apoptosis: a mechanism for trophic survival signaling
    Andreas Bergmann
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Dev Cell 2:159-70. 2002
    ..Therefore, a direct pathway linking a specific extracellular survival factor with a caspase-based death program has been established...
  19. ncbi Signaling from germ cells mediated by the rhomboid homolog stet organizes encapsulation by somatic support cells
    Cordula Schulz
    Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5329, USA
    Development 129:4523-34. 2002
    ..The micro-environment provided by the surrounding somatic cells may, in turn, regulate differentiation of the germ cells they enclose...
  20. ncbi Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye
    Lihui Yang
    Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Dev Cell 4:359-69. 2003
    ..Our results suggest that, rather than triggering all or none responses, EGFR coordinates partially independent processes as the eye differentiates...
  21. ncbi Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline
    Marco Gallio
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 106 96 Stockholm, Sweden
    Development 131:3605-14. 2004
    ..We propose that Egfr activation provides a necessary switch for the interpretation of a yet unknown repellent function of the midline...
  22. pmc Drosophila damaged DNA-binding protein 1 is an essential factor for development
    Kei ichi Takata
    Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda shi, Chiba ken 278 8510, Japan
    Genetics 168:855-65. 2004
    ..Correspondingly, all of the D-DDB1 gene knocked-out flies also died. D-DDB1 therefore appears to be an essential development-associated factor in a multicellular organism...
  23. ncbi Computational models for neurogenic gene expression in the Drosophila embryo
    Robert P Zinzen
    Center for Integrative Genomics, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 3204, USA
    Curr Biol 16:1358-65. 2006
    ..We propose that binding site occupancy is the key rate-limiting step for establishing localized patterns of gene expression in the early Drosophila embryo...
  24. ncbi A wave of EGFR signaling determines cell alignment and intercalation in the Drosophila tracheal placode
    Mayuko Nishimura
    RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Kobe 650 0047, Japan
    Development 134:4273-82. 2007
    ....
  25. pmc Drosophila EGFR signalling is modulated by differential compartmentalization of Rhomboid intramembrane proteases
    Shaul Yogev
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    EMBO J 27:1219-30. 2008
    We explore the role of differential compartmentalization of Rhomboid (Rho) proteases that process the Drosophila EGF receptor ligands, in modulating the amount of secreted ligand and consequently the level of EGF receptor (EGFR) ..
  26. pmc Regulation of neurogenesis and epidermal growth factor receptor signaling by the insulin receptor/target of rapamycin pathway in Drosophila
    Helen McNeill
    The Wolfson Centre for Age Related Diseases, King s College, London SE1 1UL, United Kingdom
    Genetics 179:843-53. 2008
    ..Together these data suggest that InR/TOR signaling regulates the timing of differentiation through modulation of EGFR target genes in developing photoreceptors...
  27. pmc Dorsal interacting protein 3 potentiates activation by Drosophila Rel homology domain proteins
    Girish S Ratnaparkhi
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, USA
    Dev Comp Immunol 32:1290-300. 2008
    ..Chromatin immunoprecipitation experiments in S2 cells indicate the presence of Dip3 at the promoters of these genes, and this binding requires the presence of Rel proteins at these promoters...
  28. ncbi The single-minded gene of Drosophila is required for the expression of genes important for the development of CNS midline cells
    J R Nambu
    Department of Biology, University of California, Los Angeles 90024
    Cell 63:63-75. 1990
    ....
  29. ncbi Dorsoventral patterning in Drosophila oogenesis
    T Schupbach
    Department of Molecular Biology, Princeton University, New Jersey 08544
    Curr Opin Genet Dev 4:502-7. 1994
    ..This localized signal from the oocyte to the follicle cells appears to initiate a cascade of events leading to dorsal follicle cell differentiation, and delimiting and orienting the future dorsoventral axis of the embryo...
  30. ncbi Short-range repression permits multiple enhancers to function autonomously within a complex promoter
    S Gray
    Department of Biology, University of California at San Diego, La Jolla 92093 0322
    Genes Dev 8:1829-38. 1994
    ..sna is not a dedicated repressor but, instead, appears to block disparate activators. We discuss the importance of quenching as a means of permitting separate enhancers to function autonomously within a complex promoter...
  31. ncbi brother of rhomboid, a rhomboid-related gene expressed during early Drosophila oogenesis, promotes EGF-R/MAPK signaling
    A Guichard
    Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093 0349, USA
    Dev Biol 226:255-66. 2000
    The Drosophila rhomboid (rho) gene participates in localized activation of EGF-receptor signaling in various developmental settings...
  32. ncbi Ras pathway specificity is determined by the integration of multiple signal-activated and tissue-restricted transcription factors
    M S Halfon
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    Cell 103:63-74. 2000
    ..The integration of Pointed with the combinatorial effects of dTCF, Mad, Twist, and Tinman determines inductive Ras signaling specificity in muscle and heart development...
  33. pmc A family of Rhomboid intramembrane proteases activates all Drosophila membrane-tethered EGF ligands
    Sinisa Urban
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 21:4277-86. 2002
    ..This is distinct from the metalloprotease-induced activation of mammalian EGF-like ligands...
  34. ncbi Ventral dominance governs sequential patterns of gene expression across the dorsal-ventral axis of the neuroectoderm in the Drosophila embryo
    John Cowden
    Department of Molecular and Cell Biology, Division of Genetics and Development, 401 Barker Hall, University of California, Berkeley, CA 94720, USA
    Dev Biol 262:335-49. 2003
    ....
  35. pmc Coordinate enhancers share common organizational features in the Drosophila genome
    Albert Erives
    Center for Integrative Genomics, Department of Molecular and Cell Biology, Division of Genetics and Development, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 101:3851-6. 2004
    ..Organized binding sites should facilitate the identification of regulatory codes that link primary DNA sequence information with predicted patterns of gene activity...
  36. ncbi Interactions between the Notch, EGFR, and decapentaplegic signaling pathways regulate vein differentiation during Drosophila pupal wing development
    Sol Sotillos
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Dev Dyn 232:738-52. 2005
    ..Once dpp expression is initiated, Dpp and EGFR activities in the provein maintain each other and, in cooperation, determine vein cell differentiation...
  37. pmc Evolution acts on enhancer organization to fine-tune gradient threshold readouts
    Justin Crocker
    Department of Biological Sciences, Dartmouth College, Hanover, NH, USA
    PLoS Biol 6:e263. 2008
    ..Our study demonstrates that equivalence classes of CRMs are powerful tools for detecting lineage-specific adaptations by gene regulatory sequences...
  38. pmc Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling
    Yanlan Mao
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Development 136:473-81. 2009
    ..Our results introduce a new regulatory interaction between an adhesion protein and a Drosophila signalling pathway and highlight the extent to which the EGFR pathway must be regulated at multiple levels...
  39. ncbi The Drosophila spitz gene encodes a putative EGF-like growth factor involved in dorsal-ventral axis formation and neurogenesis
    B J Rutledge
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115
    Genes Dev 6:1503-17. 1992
    ..We propose that spi may act as a ligand for cell-specific receptors, possibly rhomboid and/or the Drosophila EGF receptor homolog...
  40. ncbi rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster
    E Bier
    Howard Hughes Medical Institute, University of California, San Francisco 94143
    Genes Dev 4:190-203. 1990
    rhomboid (rho) belongs to a group of four genes involved in the elaboration of positional information at a ventrolateral level in the Drosophila embryo...
  41. ncbi A group of genes required for pattern formation in the ventral ectoderm of the Drosophila embryo
    U Mayer
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, FRG
    Genes Dev 2:1496-511. 1988
    Mutations in the genes spitz (spi), Star (S), single-minded (sim), pointed (pnt), rhomboid (rho) (all zygotic), and sichel (sic) (maternal), collectively called the spitz group, cause similar pattern alterations in ventral ectodermal ..
  42. ncbi Secreted Spitz triggers the DER signaling pathway and is a limiting component in embryonic ventral ectoderm determination
    R Schweitzer
    Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel
    Genes Dev 9:1518-29. 1995
    ..Spatially restricted processing of Spitz may be responsible for this graded activation. The Rhomboid (Rho) and Star proteins were suggested, on the basis of genetic interactions, to act as modulators of DER signaling...
  43. ncbi The relationship between ovarian and embryonic dorsoventral patterning in Drosophila
    S Roth
    Department of Molecular Biology, Princeton University, NJ 08544
    Development 120:2245-57. 1994
    ....
  44. ncbi Blistered: a gene required for vein/intervein formation in wings of Drosophila
    D Fristrom
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Development 120:2661-71. 1994
    ..rhove mutations (lacking rhomboid in wings) suppress the excess vein formation and associated with bs. Conversely, rho expression in prepupal and pupal bs wings is expanded in the regions of increased vein formation...
  45. ncbi Signalling pathways initiated by receptor protein tyrosine kinases in Drosophila
    N Perrimon
    Howard Hughes Medical Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115
    Curr Opin Cell Biol 6:260-6. 1994
    ..e. Grb2, Sos, Ras-Gap, p21ras, Raf, MEK and MAPK, genetic studies have suggested that two novel proteins, the protein tyrosine phosphatase (PTPase) Csw and the transmembrane protein Rho, are involved in RPTK signalling.
  46. ncbi The sevenless signalling cassette mediates Drosophila EGF receptor function during epidermal development
    F J Diaz-Benjumea
    Zoologisches Institut, Universitat Zurich, Switzerland
    Development 120:569-78. 1994
    ..It appears that these components form a signalling cassette, which mediates all aspects of DER signalling but that is not required for other signalling processes during epidermal development...
  47. ncbi Binding affinities and cooperative interactions with bHLH activators delimit threshold responses to the dorsal gradient morphogen
    J Jiang
    Department of Biology, University of California, San Diego, La Jolla 92093 0322
    Cell 72:741-52. 1993
    ..We discuss how enhancers serve as templates to bring weakly interacting regulatory factors into close proximity so that they can function combinatorially to activate and repress transcription...
  48. pmc The gap protein knirps mediates both quenching and direct repression in the Drosophila embryo
    D N Arnosti
    Department of Biology, UCSD, La Jolla 92093 0347, USA
    EMBO J 15:3659-66. 1996
    ..Our studies suggest that short-range repression represents a flexible form of gene regulation, exhibiting enhancer- or promoter-specific effects depending on the location of repressor binding sites...
  49. ncbi The Drosophila embryonic midline is the site of Spitz processing, and induces activation of the EGF receptor in the ventral ectoderm
    M Golembo
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Development 122:3363-70. 1996
    ..The Rhomboid and Star proteins are also expressed and required in the midline. The ectodermal defects of spitz, rho or Star mutant embryos could be rescued by inducing the expression of the respective normal genes only in the ..
  50. ncbi Pointed, an ETS domain transcription factor, negatively regulates the EGF receptor pathway in Drosophila oogenesis
    A M Morimoto
    Department of Biochemistry, University of Washington, Seattle 98195, USA
    Development 122:3745-54. 1996
    ....
  51. ncbi The Drosophila decapentaplegic and short gastrulation genes function antagonistically during adult wing vein development
    K Yu
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093, USA
    Development 122:4033-44. 1996
    ..in dpp and sog mutant wings suggests that dpp promotes vein fates indirectly by activating the vein gene rhomboid (rho), and that sog functions by blocking an autoactivating Dpp feedback loop...
  52. ncbi Expression and function of decapentaplegic and thick veins during the differentiation of the veins in the Drosophila wing
    J F de Celis
    Department of Genetics, University of Cambridge, UK
    Development 124:1007-18. 1997
    ..acting through its receptor Thick veins, activates vein differentiation and restricts expression of both veinlet and the Notch-ligand Delta to the developing veins...
  53. ncbi A thousand and one roles for the Drosophila EGF receptor
    R Schweitzer
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Trends Genet 13:191-6. 1997
    ..The cellular mechanisms that translate EGFR activation to discrete cell fates are now the focus of intense studies...
  54. ncbi MAP kinase in situ activation atlas during Drosophila embryogenesis
    L Gabay
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Development 124:3535-41. 1997
    ..Since the antibody was raised against the phosphorylated form of a conserved ERK peptide containing the TEY motif, this approach is applicable to a wide spectrum of multicellular organisms...
  55. ncbi Ectopic activation of torpedo/Egfr, a Drosophila receptor tyrosine kinase, dorsalizes both the eggshell and the embryo
    A M Queenan
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, NJ 08544, USA
    Development 124:3871-80. 1997
    ..The expression of genes known to respond to top/Egfr activation, argos (aos), kekkon1 (kek 1) and rhomboid (rho), was also expanded in the presence of the lambda top construct...
  56. ncbi Genetic interactions and cell behaviour in blistered mutants during proliferation and differentiation of the Drosophila wing
    F Roch
    Centro de Biologia Molecular Severo Ochoa, Madrid, Spain
    Development 125:1823-32. 1998
    ..We observe strong genetic interactions between blistered, veinlet and genes of the Ras signaling cascade...
  57. pmc Seven-up, the Drosophila homolog of the COUP-TF orphan receptors, controls cell proliferation in the insect kidney
    B Kerber
    Max Planck Institut fur biophysikalische Chemie, Abteilung Molekulare Entwicklungsbiologie, 37077 Gottingen, Germany
    Genes Dev 12:1781-6. 1998
    ..Seven-up in turn is capable of regulating the transcription of cell cycle regulators...
  58. ncbi EGFR signaling is required for the differentiation and maintenance of neural progenitors along the dorsal midline of the Drosophila embryonic head
    K Dumstrei
    Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA
    Development 125:3417-26. 1998
    ..Overactivity of EGFR signaling, as achieved by heat-shock-driven activation of a wild-type rhomboid (rho) construct, or by loss of function of argos (aos) or yan, results in an hyperplasia and deformity of the head ..
  59. ncbi Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs
    B Biehs
    Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093 0349, USA ucsd edu
    Development 125:4245-57. 1998
    ..We present a model in which different A/P boundaries organize vein-specific genetic programs to govern the development of individual veins...
  60. ncbi Regulation of EGF receptor signaling establishes pattern across the developing Drosophila retina
    S A Spencer
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, Campus Box 8103, St Louis, MO 63110, USA
    Development 125:4777-90. 1998
    ..Finally, secretion by the proneural clusters of Argos represses DER activity in less mature regions to create a new pattern of R8s. Propagation of this process anteriorly results in a retina with a precise array of maturing ommatidia...
  61. ncbi Transcriptional repression by the Drosophila giant protein: cis element positioning provides an alternative means of interpreting an effector gradient
    G F Hewitt
    Department of Biochemistry, Michigan State University, East Lansing, MI 48823, USA
    Development 126:1201-10. 1999
    ..We also demonstrate that a chimeric Gal4-Giant protein lacking the basic/zipper domain can specifically repress reporter genes, suggesting that the Giant effector domain is an autonomous repression domain...
  62. ncbi rhomboid and Star interact synergistically to promote EGFR/MAPK signaling during Drosophila wing vein development
    A Guichard
    Department of Biology, University of California San Diego, La Jolla CA 92093 0349, USA
    Development 126:2663-76. 1999
    ..Egfr encodes the Drosophila EGF-Receptor, spitz (spi) and vein (vn) encode EGF-related ligands, and rhomboid (rho) and Star (S) encode membrane proteins...
  63. ncbi Antagonism of EGFR and notch signalling in the reiterative recruitment of Drosophila adult chordotonal sense organ precursors
    P zur Lage
    Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Edinburgh, EH9 3JR, UK
    Development 126:3149-57. 1999
    ..SOP recruitment is reiterative because the inductive signal comes from previously recruited SOPs...
  64. ncbi Combined activities of Gurken and decapentaplegic specify dorsal chorion structures of the Drosophila egg
    F Peri
    Institut fur Entwicklungsbiologie, Universitat zu Koln, D 50923 Köln, Germany
    Development 127:841-50. 2000
    ..This intersection also induces rhomboid expression and thereby initiates the positive feedback loop of EGF receptor activation, which positions the dorsal appendages along the dorsal-ventral egg axis...
  65. pmc A family of rhomboid-like genes: Drosophila rhomboid-1 and roughoid/rhomboid-3 cooperate to activate EGF receptor signaling
    J D Wasserman
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Genes Dev 14:1651-63. 2000
    ..Rhomboid-1 and Roughoid/Rhomboid-3 act in the signal-emitting not signal-receiving cell, supporting the idea that Spitz activation is regulated by Rhomboid-like molecules...
  66. ncbi Drawing lines in the Drosophila wing: initiation of wing vein development
    E Bier
    Division of Biology, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla 92093 0349, USA
    Curr Opin Genet Dev 10:393-8. 2000
    ..As a consequence of this constraint, cells lying in a narrow line immediately outside the signal-producing domain are the only cells that can respond to the signal by activating expression of vein-promoting genes...
  67. ncbi Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila
    C A Conley
    Department of Zoology, University of Wisconsin, Madison, WI 53706, USA
    Development 127:3947-59. 2000
    ..These features strongly suggest that Crossveinless 2 acts extracelluarly or in the secretory pathway to directly potentiate Dpp or Gbb signaling...
  68. ncbi Feedback control of intercellular signalling in development
    M Freeman
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 408:313-9. 2000
    ..These feedback interactions can impart precision, robustness and versatility to intercellular signals. Feedback failure can cause disease...
  69. ncbi The EGF receptor and N signalling pathways act antagonistically in Drosophila mesothorax bristle patterning
    J Culi
    Centro de Biologia Molecular Severo Ochoa, CSIC and UAM, Cantoblanco, Madrid, Spain
    Development 128:299-308. 2001
    ..Excess EGFR signalling can overrule lateral inhibition and allow adjacent cells to become SMCs and sensory organs. Thus, the EGFR and Notch pathways act antagonistically in notum macrochaetae determination...
  70. ncbi Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development
    I Castanon
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Development 128:3145-59. 2001
    ..Moreover, this work provides a paradigm for understanding how the same protein controls a sequence of events within a single lineage...
  71. pmc Intracellular trafficking by Star regulates cleavage of the Drosophila EGF receptor ligand Spitz
    Rachel Tsruya
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Genes Dev 16:222-34. 2002
    ..We show that the Star and Rhomboid (Rho) proteins are necessary for Spi cleavage in Drosophila cells...
  72. ncbi Subdivision of the Drosophila wing imaginal disc by EGFR-mediated signaling
    Myriam Zecca
    Department of Genetics and Development, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA
    Development 129:1357-68. 2002
    ..Hence, as the disc grows, the DV compartment boundary can shift ventrally, beyond the range of the instructive EGFR signal(s), in contrast to the notum-wing boundary, which continues to be defined by EGFR input...
  73. ncbi abdominal A specifies one cell type in Drosophila by regulating one principal target gene
    Véronique Brodu
    Medical Research Council, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
    Development 129:2957-63. 2002
    ..use Hox mutant rescue assays to demonstrate that this function of abdA can be reconstituted by providing Rhomboid (Rho), a processing factor for the EGF receptor ligand, secreted Spitz...
  74. pmc Keren, a new ligand of the Drosophila epidermal growth factor receptor, undergoes two modes of cleavage
    Aderet Reich
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    EMBO J 21:4287-96. 2002
    ..To allow cleavage, Star transports Spi to the Golgi, where it undergoes cleavage by Rhomboid (Rho)...
  75. ncbi Conservation of intramembrane proteolytic activity and substrate specificity in prokaryotic and eukaryotic rhomboids
    Sinisa Urban
    MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
    Curr Biol 12:1507-12. 2002
    ..Moreover, a function for Rhomboids in activating intercellular signaling appears to have evolved early...
  76. ncbi Vein-positioning in the Drosophila wing in response to Hh; new roles of Notch signaling
    Michele Crozatier
    Centre de Biologie du Developpement, UMR 5547 and IFR 109 CNRS UPS, 118 route de Narbonne, 31062, Toulouse Cedex 4, France
    Mech Dev 120:529-35. 2003
    ..Observation that the vein marker rhomboid (rho) is expressed at the centre of wider vein-competent domains led to propose that narrow vein primordia form first, ..
  77. ncbi Substrate specificity of rhomboid intramembrane proteases is governed by helix-breaking residues in the substrate transmembrane domain
    Sinisa Urban
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Mol Cell 11:1425-34. 2003
    ..These results provide an explanation of how rhomboid proteases achieve specificity, and allow some rhomboid substrates to be predicted from sequence information...
  78. ncbi Species-specific activation of EGF receptor signaling underlies evolutionary diversity in the dorsal appendage number of the genus Drosophila eggshells
    Yukio Nakamura
    Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Mech Dev 120:897-907. 2003
    ..Here we show that during oogenesis the expression patterns of rhomboid (rho) and argos (aos), positive and negative regulators of EGFR signaling, respectively, were substantially different ..
  79. ncbi Multiple signaling pathways and a selector protein sequentially regulate Drosophila wing development
    Shian Jang Yan
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Development 131:285-98. 2004
    ..Such a mechanism is possibly conserved in the appendage outgrowth of other arthropods and vertebrates...
  80. ncbi A regulatory code for neurogenic gene expression in the Drosophila embryo
    Michele Markstein
    Department of Molecular and Cellular Biology, Division of Genetics and Development, 401 Barker Hall, University of California, Berkeley, CA 94720, USA
    Development 131:2387-94. 2004
    ..We discuss the prospects for deciphering regulatory codes that link primary DNA sequence information with predicted patterns of gene expression...
  81. pmc Mechanism of intramembrane proteolysis investigated with purified rhomboid proteases
    Marius K Lemberg
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 24:464-72. 2005
    ..We identify a motif shared between rhomboids and the recently discovered derlins, which participate in translocation of misfolded membrane proteins...
  82. ncbi Juxtaposition between two cell types is necessary for dorsal appendage tube formation
    Ellen J Ward
    Department of Genome Sciences, University of Washington, Box 357730, Seattle, WA 98195 7730, USA
    Mech Dev 122:241-55. 2005
    ..Two distinct cell types contribute to dorsal appendage formation: cells expressing the rhomboid-lacZ (rho-lacZ) marker form the ventral floor of the tube and cells expressing high levels of the transcription factor Broad ..
  83. ncbi Hox-controlled reorganisation of intrasegmental patterning cues underlies Drosophila posterior spiracle organogenesis
    Samir Merabet
    Laboratoire de Genetique et Physiologie du Developpement, IBDM, CNRS, Universite de la Mediterranee, Parc Scientifique de Luminy, Case 907, 13288, Marseille Cedex 09, France
    Development 132:3093-102. 2005
    ..These changes in expression patterns confer axial specificity to otherwise reiteratively used segmental patterning cues, linking intrasegmental polarity and acquisition of segment identity...
  84. ncbi Egfr is essential for maintaining epithelial integrity during tracheal remodelling in Drosophila
    Carolina Cela
    Institut de Biologia Molecular de Barcelona IBMB Samitier 1 5, 08028 Barcelona, Spain
    Development 133:3115-25. 2006
    ..This finding establishes a link between a developmental pathway governing tracheal formation and cell adhesiveness...
  85. pmc Comprehensive identification of Drosophila dorsal-ventral patterning genes using a whole-genome tiling array
    Frederic Biemar
    Division of Genetics and Development, Department of Molecular Cell Biology, Center for Integrative Genomics, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 103:12763-8. 2006
    ..We discuss the potential functions of these recently identified genes and suggest that intronic enhancers are a common feature of the DV gene network...
  86. ncbi Epithelial cell adhesion in the developing Drosophila retina is regulated by Atonal and the EGF receptor pathway
    Katherine E Brown
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Dev Biol 300:710-21. 2006
    ..These observations define a new function for EGFR signalling in eye development and illustrate a mechanism for the control of epithelial morphology by developmental signals...
  87. pmc Enzymatic analysis of a rhomboid intramembrane protease implicates transmembrane helix 5 as the lateral substrate gate
    Rosanna P Baker
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 507 Preclinical Teaching Building, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:8257-62. 2007
    ..Moreover, our mutagenesis also underscores the importance of other residue interactions within the enzyme that warrant further scrutiny...
  88. ncbi Activation of EGFR and ERK by rhomboid signaling regulates the consolidation and maintenance of sleep in Drosophila
    Krisztina Foltenyi
    Department of Biology, University of California San Diego, 9500 Gilman Dr, La Jolla, California 92093, USA
    Nat Neurosci 10:1160-7. 2007
    ..Our results demonstrate that rhomboid (Rho)- and Star-mediated activation of EGFR and ERK signaling increases sleep in a dose-dependent manner, and that ..
  89. pmc Egfr/Ras signaling regulates DE-cadherin/Shotgun localization to control vein morphogenesis in the Drosophila wing
    David D O'Keefe
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Dev Biol 311:25-39. 2007
    ..Ras, therefore, regulates both the transcriptional responses necessary for vein cell identity, and the cell adhesive changes that determine vein and intervein cell morphology...
  90. pmc RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
    Julia Zeitlinger
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:1512-6. 2007
    ..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
  91. ncbi Drosophila eggshell is patterned by sequential action of feedforward and feedback loops
    Nir Yakoby
    Department of Chemical Engineering and Lewis Sigler Institute for Integrative Genomics, Princeton University, NJ 08544, USA
    Development 135:343-51. 2008
    ..This mechanism demonstrates how complex patterns of gene expression can emerge from simple inputs, through the interaction of regulatory network motifs...
  92. pmc Hox and senseless antagonism functions as a molecular switch to regulate EGF secretion in the Drosophila PNS
    David Li-Kroeger
    Division of Developmental Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    Dev Cell 15:298-308. 2008
    ..As the vertebrate senseless homolog is essential for neural development as well as hematopoiesis, we propose Hox-Senseless antagonism will broadly control cell fate decisions...
  93. doi Distinct activation patterns of EGF receptor signaling in the homoplastic evolution of eggshell morphology in genus Drosophila
    Tatsuo Kagesawa
    Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Mech Dev 125:1020-32. 2008
    ..Our results suggest that acquisition of the homoplastic two-DA characteristic could be explained by modifications of the EGFR signaling system in the genus Drosophila that occurred independently and at least twice during evolution...
  94. doi Osa, a subunit of the BAP chromatin-remodelling complex, participates in the regulation of gene expression in response to EGFR signalling in the Drosophila wing
    Ana Terriente-Félix
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Dev Biol 329:350-61. 2009
    ....
  95. pmc Feedback control of the EGFR signaling gradient: superposition of domain-splitting events in Drosophila oogenesis
    Jeremiah J Zartman
    Lewis Sigler Institute and Department of Chemical Engineering, Carl Icahn Laboratory, Washington Road, Princeton University, Princeton, NJ 08544, USA
    Development 136:2903-11. 2009
    ..Thus, the processes that define the two-domain patterns of Broad and EGFR activation are distinct; their actions are separated in time and have different effects on eggshell morphology...
  96. doi Graded Egfr activity patterns the Drosophila eggshell independently of autocrine feedback
    Jean François Boisclair Lachance
    Department of Biology, McGill University, 1205 Doctor Penfield Avenue, Montreal, Quebec, Canada
    Development 136:2893-902. 2009
    ....
  97. pmc Sequence-specific intramembrane proteolysis: identification of a recognition motif in rhomboid substrates
    Kvido Strisovsky
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Mol Cell 36:1048-59. 2009
    ..Our work demonstrates that intramembrane proteases can be sequence specific and that genome-wide substrate prediction based on their recognition motifs is feasible...
  98. pmc Dynamic evolution of precise regulatory encodings creates the clustered site signature of enhancers
    Justin Crocker
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Nat Commun 1:99. 2010
    ....
  99. pmc Capicua DNA-binding sites are general response elements for RTK signaling in Drosophila
    Leiore Ajuria
    Institut de Biologia Molecular de Barcelona CSIC, Parc Cientific de Barcelona, Barcelona, Spain
    Development 138:915-24. 2011
    ..We conclude that Capicua octamers are general response elements for RTK signaling in Drosophila...
  100. pmc Insights into Hox protein function from a large scale combinatorial analysis of protein domains
    Samir Merabet
    Institut de Biologie du Developpement de Marseille Luminy, UMR6216 CNRS, Parc Scientifique de Luminy, Case 907, Marseille, France
    PLoS Genet 7:e1002302. 2011
    ....
  101. ncbi Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila
    J R Lee
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 107:161-71. 2001
    ..Rhomboid-1 is localized in the Golgi, where it promotes the cleavage of Spitz. This defines a novel growth factor release mechanism that is distinct from metalloprotease-dependent shedding from the cell surface...

Research Grants19

  1. CONTROL OF TGF-BETA SIGNALING IN DROSOPHILA BY BRINKER
    Gerard Campbell; Fiscal Year: 2004
    ..Consequently, the present research should have direct relevance to our understanding of how defects in TGF-B signaling can lead to disease. ..
  2. High-Volume Confocal Imaging System
    Stephen Crews; Fiscal Year: 2005
    ..The Pascal will allow the labs to expeditiously and comprehensively study the cellular basis of embryonic development in combination with existing bright-field and spinning disk microscopes. ..
  3. MSM: CR Multiscale analysis of epithelial patterning: m*
    Stanislav Shvartsman; Fiscal Year: 2007
    ..The project will bring together researchers in biology, engineering and mathematics in an interdisciplinary research program aimed at bringing about new understanding of EGFR system. ..
  4. CELL FATE DETERMINATION IN THE MESODERM OF DROSOPHILA
    Mary Baylies; Fiscal Year: 2008
    ..Our work in Drosophila on the mechanisms and targets of these regulators' action will provide insight as to the underlying causes of these diseases and will help to reveal potential therapies. ..
  5. The structural basis for APP cleavability
    Ya Ha; Fiscal Year: 2009
    ..abstract_text> ..
  6. MECHANISMS OF TRANSCRIPTIONAL REPRESSION IN DROSOPHILA
    GERARD L CAMPBELL; Fiscal Year: 2010
    ..Consequently, the proposed studies should provide insights into diseases such as these, potentially leading to improved diagnosis and treatment. ..
  7. Synaptic Glutamate Release in Drosphila
    Aaron DiAntonio; Fiscal Year: 2008
    ..The results of these experiments will provide novel and fundamental information on the mechanisms by which VGLUT contributes to the development and function of glutamatergic synapses. ..
  8. The Role of Presenilin in Gamma-Secretase Activity
    Michael Wolfe; Fiscal Year: 2009
    ..for substrate-protease recognition? (3) What is the role of Aph-1 and NCT in the assembly and activity of the protease complex? (4) How do mutations in PS that cause familial AD alter the specificity and activity of gamma-secretase? ..
  9. Biology of Serine Proteases and Serpins
    Carl Hashimoto; Fiscal Year: 2010
    ..abstract_text> ..
  10. Molecular Genetics of Midline Glial Development
    Stephen T Crews; Fiscal Year: 2010
    ..abstract_text> ..
  11. Mechanisms Regulating Myoblast Fusion in Drosophila
    Mary K Baylies; Fiscal Year: 2010
    ....
  12. Development of tissue-specific cell lines in Drosophila
    Amanda Simcox; Fiscal Year: 2009
    ..Subsequent use of the cell lines by individual investigators to study diverse biological problems is expected to impact our understanding of human disease. ..
  13. Structure and mechanism of signal peptide peptidase
    Michael S Wolfe; Fiscal Year: 2010
    ..A structure of SPP would provide tremendous insight into the workings of an unusual new class of enzymes that are critical to both biology and medicine. ..
  14. Molecular and genetic analysis of sanpodo
    James B Skeath; Fiscal Year: 2010
    ..Such insight should help us understand the etiology of diseases in which this process is de-regulated and design new methods to treat these diseases. ..
  15. Regulation of Cell Migration by the APC-Microtubule Complex
    William Nelson; Fiscal Year: 2009
    ..abstract_text> ..
  16. SYNAPSE STRUCTURE AND DEVELOPMENT
    W Nelson; Fiscal Year: 2001
    ..This research will aid in the prevention of psychiatric illness and the development of therapies for afflicted individuals. ..
  17. Genome wide analysis and modeling of signal integration in the Drosophila ovary
    Stanislav Shvartsman; Fiscal Year: 2009
    ..Given the highly conserved nature of these signaling pathways, our results will provide quantitative insights into the mechanisms of signaling crosstalk in developing and adult tissues. ..
  18. Cytoskeleton Coordination in Neuronal Morphogenesis
    W Nelson; Fiscal Year: 2005
    ....
  19. Vascular cell migration and VHL gene function in flies
    Tien Hsu; Fiscal Year: 2003
    ..2) To assess the relevance of the potential D-VHL interacting proteins and complete the ongoing yeast 2-hybrid screen for D-VHL interacting proteins. (4) Generating genomic D-VHL mutant. ..