Genomes and Genes
Gene Symbol: okr
Alias: CG3736, DhR54, DmRAD54, DmRad54, Dmel\CG3736, Dmrad54, OKR, Okr, RAD54, RAD54L, Rad54, Ras54, rad54, CG3736 gene product from transcript CG3736-RA, CG3736-PA, okr-PA
Species: fruit fly
- The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombinationR Kooistra
Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University, The Netherlands
Mol Cell Biol 17:6097-104. 1997..Here the isolation and functional characterization of the RAD54 homolog of the fruit fly Drosophila melanogaster, DmRAD54, are described. The putative Dmrad54 protein displays 46 to 57% identity to its homologs from yeast and mammals...
- The Drosophila zinc finger protein trade embargo is required for double strand break formation in meiosisCathleen M Lake
Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
PLoS Genet 7:e1002005. 2011..We speculate that Trem may act by either directing the binding of Mei-P22 to preferred sites of DSB formation or by altering chromatin structure in a manner that allows Mei-P22 to form foci...
- Meiotic recombination provokes functional activation of the p53 regulatory networkWan Jin Lu
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Science 328:1278-81. 2010..Our findings establish a physiological role for p53 in meiosis and suggest that tumor-suppressive functions may have been co-opted from primordial activities linked to recombination...
- Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formationEric F Joyce
Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
Genetics 181:39-51. 2009..Interestingly, PCH2-dependent delays in prophase may allow additional crossovers to form...
- Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpointMartha Klovstad
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
PLoS Genet 4:e31. 2008..In addition, Brca2 co-immunoprecipitates with the checkpoint protein Rad9, suggesting a direct role for Brca2 in the transduction of the meiotic recombination checkpoint signal...
- Multiple-pathway analysis of double-strand break repair mutations in DrosophilaDena M Johnson-Schlitz
Department of Genetics, University of Wisconsin, Madison, Wisconsin, United States of America
PLoS Genet 3:e50. 2007....
- An essential role for Drosophila hus1 in somatic and meiotic DNA damage responsesUri Abdu
Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben Gurion University, Beer Sheva, 84105, Israel
J Cell Sci 120:1042-9. 2007..These results demonstrate that hus1 is essential for the activation of the meiotic checkpoint and that hus1 is also required for the organization of the oocyte DNA, a function that might be independent of the meiotic checkpoint...
- Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila femalesS Mehrotra
Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
PLoS Genet 2:e200. 2006..From a comparison of the frequency of gamma-His2Av foci and crossovers, it appears that Drosophila females have only a weak mechanism to ensure a crossover in the presence of a low number of DSBs...
- Mutations in the extra sex combs and Enhancer of Polycomb genes increase homologous recombination in somatic cells of Drosophila melanogasterAngela M Holmes
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
Genetics 172:2367-77. 2006..We propose that esc and E(Pc) mutants enhance homologous recombination by modulating the histone acetylation status of histone H4 at the double-strand break...
- Lig4 and rad54 are required for repair of DNA double-strand breaks induced by P-element excision in DrosophilaRon J Romeijn
Department of Toxicogenetics, Leiden University Medical Center LUMC, 2333 AL, Leiden, The Netherlands
Genetics 169:795-806. 2005..the repair of these breaks, the w(hd) P-element was mobilized in flies carrying mutant alleles of either lig4 or rad54. The survival of both lig4- and rad54-deficient males was reduced to 25% in comparison to the wild type, ..
- Studies on crossover-specific mutants and the distribution of crossing over in Drosophila femalesR Bhagat
Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
Cytogenet Genome Res 107:160-71. 2004..This may be the activation of a regulatory system that ensures at least one crossover per chromosome, and which compensates for an absence of crossovers by attempting to generate them at random locations...
- A large-scale screen for mutagen-sensitive loci in DrosophilaAnne Laurencon
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
Genetics 167:217-31. 2004..At least one allele of each new gene identified in this study is available at the Bloomington Stock Center...
- The Drosophila melanogaster DNA Ligase IV gene plays a crucial role in the repair of radiation-induced DNA double-strand breaks and acts synergistically with Rad54Marcin M Gorski
Department of Toxicogenetics, Leiden University Medical Center, 2333 AL, Leiden, The Netherlands
Genetics 165:1929-41. 2003..To determine the relative contribution of NHEJ and homologous recombination (HR) in Drosophila, Lig4; Rad54 double-mutant flies were generated...
- The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosisUri Abdu
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Genetics 165:197-204. 2003..As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1...
- The Synaptonemal complex component C(2)M regulates meiotic crossing over in DrosophilaElizabeth A Manheim
Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
Curr Biol 13:276-85. 2003..Previous studies have suggested that the SC is required for meiotic crossing over in Drosophila. However, only one component of this structure, C(3)G, has been identified in Drosophila...
- Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/MnkUri Abdu
HHMI, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
Curr Biol 12:1645-51. 2002..However, the components of this checkpoint in flies have not been completely elucidated...
- Activation of a meiotic checkpoint regulates translation of Gurken during Drosophila oogenesisA Ghabrial
HHMI, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
Nat Cell Biol 1:354-7. 1999..We also show that Vasa is a target of this meiotic checkpoint, and so may mediate the checkpoint-dependent translational regulation of Gurken...
- Relationship of DNA double-strand breaks to synapsis in DrosophilaJanet K Jang
Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
J Cell Sci 116:3069-77. 2003..Furthermore, mutations in Drosophila Rad54 (okr) and Rad51 (spnB) homologs cause delayed and prolonged gamma-HIS2AV staining, suggesting that double-strand ..
- An RNAi-Based Candidate Screen for Modifiers of the CHD1 Chromatin Remodeler and Assembly Factor in Drosophila melanogasterSharon Kim
The W M Keck Science Department, Claremont McKenna College, Scripps College and Pitzer College, California 91711
G3 (Bethesda) 6:245-54. 2015..Our results indicate that CHD1 may act in opposition to other remodeling factors, including INO80, and that the recruitment of CHD1 to active genes by RTF1 is conserved in flies. ..
- Vilya, a component of the recombination nodule, is required for meiotic double-strand break formation in DrosophilaCathleen M Lake
Stowers Institute for Medical Research, Kansas City, United States
elife 4:e08287. 2015..Thus Vilya links the mechanism of DSB formation to either the selection of those DSBs that will become crossovers or to the actual process of crossing over. ..
- Corolla is a novel protein that contributes to the architecture of the synaptonemal complex of DrosophilaKimberly A Collins
Stowers Institute for Medical Research, Kansas City, Missouri 64110
Genetics 198:219-28. 2014..These observations help provide a more complete model of SC structure and function in Drosophila females...
- Aneuploidy and tumorigenesis in DrosophilaMarco Milan
ICREA, Spain Institute for Research in Biomedicine IRB Barcelona, Baldiri Reixac, 10 12, 08028 Barcelona, Spain Electronic address
Semin Cell Dev Biol 28:110-5. 2014..Here we discuss the role of aneuploidy in tumorigenesis in light of the contribution of Drosophila epithelial cancer models and propose a stress-induced tumor-promoting role of aneuploidy. ..
- Contributions of DNA repair, cell cycle checkpoints and cell death to suppressing the DNA damage-induced tumorigenic behavior of Drosophila epithelial cellsA Dekanty
Institute for Research in Biomedicine IRB Barcelona, Barcelona, Spain
Oncogene 34:978-85. 2015..The tumorigenic response of the tissue to IR is enhanced by depletion of Okra/DmRAD54 or spnA/DmRAD51--genes required for homologous recombination (HR) repair of DNA double-strand breaks in G2--and ..
- p53 activity is selectively licensed in the Drosophila stem cell compartmentAnnika Wylie
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States
elife 3:e01530. 2014..Furthermore, the findings uncover ancestral links between p53 and aberrant proliferation that are independent of DNA breaks and predate evolution of the ARF/Mdm2 axis. DOI: http://dx.doi.org/10.7554/eLife.01530.001. ..
- Sources and structures of mitotic crossovers that arise when BLM helicase is absent in DrosophilaMatthew C LaFave
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599
Genetics 196:107-18. 2014..These findings provide important new insights into sources and structures of mitotic crossovers and functions of BLM helicase. ..
- The development of a monoclonal antibody recognizing the Drosophila melanogaster phosphorylated histone H2A variant (γ-H2AV)Cathleen M Lake
Stowers Institute for Medical Research, Kansas City, Missouri 64110
G3 (Bethesda) 3:1539-43. 2013....
- Transcriptomic analysis provides insights on hexavalent chromium induced DNA double strand breaks and their possible repair in midgut cells of Drosophila melanogaster larvaeManish Mishra
Embryotoxicology Section and Environmental Toxicology Group, CSIR Indian Institute of Toxicology Research CSIR IITR, Lucknow 226001, Uttar Pradesh, India
Mutat Res 747:28-39. 2013..Along with this, DSBs generation in spn-A and okr [essential genes in homologous recombination repair (HR) pathway] mutants was also tested for the possible ..
- Cyclin G is involved in meiotic recombination repair in Drosophila melanogasterAnja C Nagel
Institut fur Genetik, Universitat Hohenheim, Garbenstr 30, 70599 Stuttgart, Germany
J Cell Sci 125:5555-63. 2012..Therefore, we propose that CycG has a role in an early step of meiotic recombination repair, thereby affecting EGFR-mediated patterning processes during oogenesis...
- DNA damage induced by industrial solid waste leachates in Drosophila melanogaster: a mechanistic approachHifzur R Siddique
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, Uttar Pradesh, India
Environ Mol Mutagen 49:206-16. 2008..protein), mus201 (deficient in excision repair protein), mus308 (deficient in postreplication repair protein), and rad54 (deficient in double strand break repair protein) than in the OregonR(+) wild-type strain...
- Genomic integrity and the repair of double-strand DNA breaksA Pastink
Sylvius Laboratory, Department of Radiation Genetics and Chemical Mutagenesis, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
Mutat Res 480:37-50. 2001..This review summarizes our current knowledge about DSB-repair with emphasis on recent progress in understanding the precise biochemical activities of individual proteins involved...
- Double-strand breaks in heterochromatin move outside of a dynamic HP1a domain to complete recombinational repairIrene Chiolo
Department of Genome Dynamics, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
Cell 144:732-44. 2011..We propose that the spatial and temporal control of DSB repair in heterochromatin safeguards genome stability by preventing aberrant exchanges between repeats...
- Localization of the Drosophila Rad9 protein to the nuclear membrane is regulated by the C-terminal region and is affected in the meiotic checkpointRotem Kadir
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
PLoS ONE 7:e38010. 2012..Thus, by studying the localization pattern of DmRad9, our study reveals that the DmRad9A C-terminal region targets the protein to the nuclear membrane, where it might play a role in response to the activation of the meiotic checkpoint...
- Extrachromosomal circular DNA of tandemly repeated genomic sequences in DrosophilaSarit Cohen
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
Genome Res 13:1133-45. 2003..Mutations in the genes okra, mus309, and mei41 did not affect eccDNA under normal conditions or following mutagen treatment, implying that eccDNA formation is different from known pathways of DNA repair...
- A genetic screen for DNA double-strand break repair mutations in DrosophilaDebbie S Wei
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 177:63-77. 2007..We show that Drosophila Rad51 and Rad54 are both required for homologous repair by gene conversion, but are dispensable for single-strand annealing repair...
- Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuageSeth D Findley
Department of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195 7350, USA
Development 130:859-71. 2003..Furthermore, maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and/or RNAi pathways, Dicer and Argonaute2, suggesting a potential connection between nuage and the microRNA-pathway...
- Relative contribution of DNA repair, cell cycle checkpoints, and cell death to survival after DNA damage in Drosophila larvaeBurnley R Jaklevic
MCD Biology, University of Colorado, Boulder, CO 80309, USA
Curr Biol 14:23-32. 2004..Cell cycle regulation and DNA repair appear essential for unicellular systems to survive DNA damage. The relative importance of these responses and apoptosis for surviving DNA damage in multicellular organisms remains unclear...
- A conserved N-terminal motif in Rad54 is important for chromatin remodeling and homologous strand pairingVassilios Alexiadis
Section of Molecular Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
J Biol Chem 279:27824-9. 2004The Swi2/Snf2-related protein Rad54 is a chromatin remodeling enzyme that is important for homologous strand pairing catalyzed by the eukaryotic recombinase Rad51...
- Strand pairing by Rad54 and Rad51 is enhanced by chromatinVassilios Alexiadis
Section of Molecular Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
Genes Dev 16:2767-71. 2002We investigated the role of chromatin in the catalysis of homologous strand pairing by Rad54 and Rad51. Rad54 is related to the ATPase subunits of chromatin-remodeling factors, whereas Rad51 is related to bacterial RecA...
- Ras1 interacts with multiple new signaling and cytoskeletal loci in Drosophila eggshell patterning and morphogenesisJ D Schnorr
Department of Biology, Whitman College, Walla Walla, Washington 99362, USA
Genetics 159:609-22. 2001..Mutations in Star and l(2)43Bb had no significant effect upon the lambdatop eggshell defect whereas smt3 and dock alleles significantly suppressed the lambdatop phenotype...
- Repair and consequences of double-strand breaks in DNAA Pastink
Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, Netherlands
Mutat Res 428:141-56. 1999
- The Drosophila melanogaster DmRAD54 gene plays a crucial role in double-strand break repair after P-element excision and acts synergistically with Ku70 in the repair of X-ray damageR Kooistra
Department of Radiation Genetics and Chemical Mutagenesis, MGC, Leiden University Medical Center, Leiden, The Netherlands
Mol Cell Biol 19:6269-75. 1999..A Drosophila melanogaster strain deficient in the RAD54 homolog DmRAD54 is characterized by increased X-ray and methyl methanesulfonate (MMS) sensitivity...
- A maternal screen for genes regulating Drosophila oocyte polarity uncovers new steps in meiotic progressionVitor Barbosa
Howard Hughes Medical Institute and the Kimmel Center for Biology and Medicine, Skirball Institute, 540 First Avenue, New York, NY 10016, USA
Genetics 176:1967-77. 2007....
- Novel genetic screen for genes involved in posterior body patterning in DrosophilaJ E Wilson
Department of Biological Sciences, Stanford University, California 94305 5020, USA
Dev Genet 19:199-209. 1996..These defects are distinct from previously described perturbations in oskar activity and provide new insights into the regulation of oskar...
- Search for DNA repair pathways in Drosophila melanogasterP G de Buendía
Instituto de Genetica, Universidad de Los Andes, Bogota, Colombia
Mutat Res 407:67-84. 1998..A possible explanation could be a weaker control of post-replication repair mechanisms in Drosophila than in yeast. It is expected that this research could help for a better understanding of repair mechanisms in complex organisms...
- The XNP remodeler targets dynamic chromatin in DrosophilaJonathan I Schneiderman
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 106:14472-7. 2009..Thus, the XNP focus appears to be a functional genetic element that can contribute to gene silencing throughout the nucleus...
- Genetic analysis of zinc-finger nuclease-induced gene targeting in DrosophilaAna Bozas
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112 5650, USA
Genetics 182:641-51. 2009..The frequency of HR dropped significantly in flies homozygous for mutations in spnA (Rad51) or okr (Rad54), two components of the invasion-mediated synthesis-dependent strand annealing (SDSA) pathway...
- Drosophila oogenesis: versatile spn doctorsJ Morris
Developmental Genetics Program, Skirball Institute, Howard Hughes Medical Institute, Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, 10016, USA
Curr Biol 9:R55-8. 1999..Genes of the spindle class, which encode double-strand break repair enzymes and RNA helicases, affect oocyte polarity and the decision whether to differentiate as an oocyte or a nurse cell...
- Metabolomic signatures of inbreeding at benign and stressful temperatures in Drosophila melanogasterKamilla Sofie Pedersen
Department of Biological Sciences, University of Aarhus, DK 8000 Aarhus C, Denmark
Genetics 180:1233-43. 2008....
- A surfeit of RAD51-like genes?J Thacker
Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire, UK OX11 0RD
Trends Genet 15:166-8. 1999..The recent cloning and functional analysis of eukaryotic recombination genes suggests that at least part of the intricate mechanism involved is conserved from bacteria to humans, but is also yielding some surprises...
- Investigations of CHD1 function in transcription and development of Drosophila melanogasterIvy E McDaniel
Joint Science Department, W M Keck Science Center, Scripps, Claremont McKenna, and Pitzer Colleges, Claremont, California 91711, USA
Genetics 178:583-7. 2008..These results clarify the roles of chromatin remodelers in transcription and provide novel insights into CHD1 function...