O-fut1

Summary

Gene Symbol: O-fut1
Description: O-fucosyltransferase 1
Alias: 4R6, AAF58290.1, CG12366, Dmel\CG12366, O-FUT1, O-fut, OFUT1, OFut1, Ofut-1, Ofut1, l(2)SH2 2260, l(2)SH2260, ntc, nti, o-fut1, CG12366 gene product from transcript CG12366-RB, CG12366-PA, CG12366-PB, O-fucosyl transferase, O-fucosyltransferase, O-fut1-PA, O-fut1-PB, neurotic
Species: fruit fly

Top Publications

  1. ncbi Regulation of notch signaling by o-linked fucose
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Cell 111:893-904. 2002
  2. ncbi Modulation of notch-ligand binding by protein O-fucosyltransferase 1 and fringe
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Biol Chem 278:42340-5. 2003
  3. ncbi neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions
    Takeshi Sasamura
    PRESTO, Japan Science and Technology Corporation, Noda, Chiba, Japan
    Development 130:4785-95. 2003
  4. ncbi An O-fucose site in the ligand binding domain inhibits Notch activation
    Liang Lei
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA
    Development 130:6411-21. 2003
  5. ncbi Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
    Science 307:1599-603. 2005
  6. ncbi Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells
    Nobuo Sasaki
    Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Genes Cells 12:89-103. 2007
  7. ncbi The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila
    Takeshi Sasamura
    Precursory Research for Embryonic Science and Technology PRESTO Science and Technology Agency, 4 1 8 Honcho Kawaguchi, Saitama, Japan
    Development 134:1347-56. 2007
  8. pmc Mapping Sites of O-Glycosylation and Fringe Elongation on Drosophila Notch
    Beth M Harvey
    From the Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794 5215
    J Biol Chem 291:16348-60. 2016
  9. pmc Frequent Somatic Mutation in Adult Intestinal Stem Cells Drives Neoplasia and Genetic Mosaicism during Aging
    Katarzyna Siudeja
    Institut Curie, 26 Rue d Ulm, F 75248 Paris, France CNRS UMR3215, F 75248 Paris, France INSERM U934, F 75248 Paris, France
    Cell Stem Cell 17:663-74. 2015
  10. pmc O-fucose monosaccharide of Drosophila Notch has a temperature-sensitive function and cooperates with O-glucose glycan in Notch transport and Notch signaling activation
    Akira Ishio
    From the Department of Biological Science and Technology, Tokyo University of Science, 6 3 1 Niijuku, Katsushika ku, Tokyo, 125 1500, The Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043
    J Biol Chem 290:505-19. 2015

Scientific Experts

  • Alvaro Glavic
  • Shinya Yamamoto
  • S B Ben
  • Francois Schweisguth
  • Tetsuya Okajima
  • Takeshi Sasamura
  • Kenji Matsuno
  • Hiroyuki O Ishikawa
  • Tomoko Yamakawa
  • Nobuo Sasaki
  • Koichi Furukawa
  • Yuta Sakaidani
  • Aiko Matsuura
  • Maiko Kanai
  • Beth M Harvey
  • Akira Ishio
  • Katarzyna Siudeja
  • Mitsutaka Ogawa
  • Omar S Akbari
  • Casper Groth
  • Allison J Bardin
  • Mark E Fortini
  • Mo Weng
  • Emiko Suzuki
  • Tomonori Ayukawa
  • Robert S Haltiwanger
  • Carolina N Perdigoto
  • Makiko Ito
  • Kosuke Murakami
  • Daita Nadano
  • Tsukasa Matsuda
  • Malin J Svensson
  • Yi Luo
  • Shiho Nakao
  • Vladislav M Panin
  • Véronique Brodu
  • Francois Ahimou
  • Hillary Moss
  • Nadia A Rana
  • Hamed Jafar-Nejad
  • Jessica Leonardi
  • Liang Lei
  • Louis Gervais
  • Junpei Kuroda
  • Maria Zande
  • Donato Stolfa
  • Sonya Nassari
  • Naoki Aoyama
  • Takuma Gushiken
  • Sonia Lameiras
  • Thomas Rio Frio
  • Virginie Bernard
  • Kenjiroo Matsumoto
  • Patricia Skorski
  • Haixia Huang
  • John M Marshall
  • Kelly D Matzen
  • Bruce A Hay
  • Michael Whitley
  • Mansi R Khanna
  • Catherine M Ward
  • Y Wang
  • Jill M Haenfler
  • Kenta Yamada
  • Cheng Yu Lee
  • Naotaka Nakazawa
  • Tomoko Nomura
  • Tatsuhiko Kondo
  • Kaoru Saigo
  • Jan Larsson
  • Katsuhisa Noda
  • Naoyuki Taniguchi
  • Toshiro Aigaki
  • Per Stenberg
  • Syunsuke Higashi
  • Eiji Miyoshi
  • Ryu Ueda
  • Wendy Vorndam
  • Kate Koles
  • Lee Peng Mok
  • Philip R Elstob
  • Boris Bardot
  • Alex P Gould
  • Cedric Wesley
  • Norbert Perrimon
  • Mikiko Ito
  • David Bilder
  • Kenneth D Irvine
  • Aiguo Xu
  • Motoo Kitagawa

Detail Information

Publications29

  1. ncbi Regulation of notch signaling by o-linked fucose
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Cell 111:893-904. 2002
    Notch and its ligands are modified by a protein O-fucosyltransferase (OFUT1) that attaches fucose to a Serine or Threonine within EGF domains...
  2. ncbi Modulation of notch-ligand binding by protein O-fucosyltransferase 1 and fringe
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    J Biol Chem 278:42340-5. 2003
    ..by protein O-fucosyltransferase 1, and down-regulation of this enzyme by RNA interference or mutation of the Ofut1 gene in Drosophila or by mutation of the Pofut1 gene in mouse prevents Notch signaling...
  3. ncbi neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions
    Takeshi Sasamura
    PRESTO, Japan Science and Technology Corporation, Noda, Chiba, Japan
    Development 130:4785-95. 2003
    ..We identify a novel maternal neurogenic gene, neurotic, and show that it is essential for Notch signalling...
  4. ncbi An O-fucose site in the ligand binding domain inhibits Notch activation
    Liang Lei
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA
    Development 130:6411-21. 2003
    Two glycosyltransferases that transfer sugars to EGF domains, OFUT1 and Fringe, regulate Notch signaling. However, sites of O-fucosylation on Notch that influence Notch activation have not been previously identified...
  5. ncbi Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding
    Tetsuya Okajima
    Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA
    Science 307:1599-603. 2005
    ..We found that in Drosophila, Protein O-fucosyltransferase 1 (OFUT1), an enzyme that glycosylates epidermal growth factor-like domains of Notch, also has a distinct Notch chaperone ..
  6. ncbi Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells
    Nobuo Sasaki
    Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278 8510, Japan
    Genes Cells 12:89-103. 2007
    ..N, but not Dl, formed a specific complex with DEcad in vivo. Finally, our results suggest that juxtacrine signaling in epithelia generally depends on the apicobasally polarized structure of epithelial cells...
  7. ncbi The O-fucosyltransferase O-fut1 is an extracellular component that is essential for the constitutive endocytic trafficking of Notch in Drosophila
    Takeshi Sasamura
    Precursory Research for Embryonic Science and Technology PRESTO Science and Technology Agency, 4 1 8 Honcho Kawaguchi, Saitama, Japan
    Development 134:1347-56. 2007
    ..We propose that O-fut1 is the first example, except for ligands, of a molecule that is required extracellularly for receptor transportation by endocytosis...
  8. pmc Mapping Sites of O-Glycosylation and Fringe Elongation on Drosophila Notch
    Beth M Harvey
    From the Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794 5215
    J Biol Chem 291:16348-60. 2016
    ..These data provide foundational information for future studies investigating the mechanisms of how O-glycosylation regulates Notch activity. ..
  9. pmc Frequent Somatic Mutation in Adult Intestinal Stem Cells Drives Neoplasia and Genetic Mosaicism during Aging
    Katarzyna Siudeja
    Institut Curie, 26 Rue d Ulm, F 75248 Paris, France CNRS UMR3215, F 75248 Paris, France INSERM U934, F 75248 Paris, France
    Cell Stem Cell 17:663-74. 2015
    ..Together, our findings reveal frequent genomic modification in adult stem cells and show that somatic genetic mosaicism has important functional consequences on aging tissues...
  10. pmc O-fucose monosaccharide of Drosophila Notch has a temperature-sensitive function and cooperates with O-glucose glycan in Notch transport and Notch signaling activation
    Akira Ishio
    From the Department of Biological Science and Technology, Tokyo University of Science, 6 3 1 Niijuku, Katsushika ku, Tokyo, 125 1500, The Department of Biological Sciences, Graduate School of Science, Osaka University, 1 1 Machikaneyama, Toyonaka, Osaka 560 0043
    J Biol Chem 290:505-19. 2015
    ..Our findings elucidate how different forms of glycosylation on a protein can influence the protein's functions. ..
  11. doi Introduction to Notch signaling
    Shinya Yamamoto
    Department of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children s Hospital, Baylor College of Medicine, Houston, TX, 77030, USA
    Methods Mol Biol 1187:1-14. 2014
    ..Further studies to understand and manipulate Notch signaling in vivo in model organisms and in clinical settings will require a combination of a number of different approaches that are discussed throughout this book. ..
  12. pmc Extracellular O-linked β-N-acetylglucosamine: Its biology and relationship to human disease
    Mitsutaka Ogawa
    Mitsutaka Ogawa, Koichi Furukawa, Tetsuya Okajima, Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya 466 0065, Japan
    World J Biol Chem 5:224-30. 2014
    ..This review summarizes the current knowledge of extracellular O-GlcNAc and its implications in the pathological processes in Adams-Oliver syndrome. ..
  13. pmc Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup
    Casper Groth
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Development 140:3018-27. 2013
    ..In addition, Catsup mutant cells exhibit elevated ER stress, suggesting that impaired zinc homeostasis causes increased levels of misfolded proteins within the secretory compartment. ..
  14. doi A synthetic gene drive system for local, reversible modification and suppression of insect populations
    Omar S Akbari
    Division of Biology, MC 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Curr Biol 23:671-7. 2013
    ..They also show that in a population in which single-locus UD(MEL) has been carried out, repeated release of wild-type males can result in population suppression, a novel method of genetic population manipulation...
  15. pmc Changes in Notch signaling coordinates maintenance and differentiation of the Drosophila larval optic lobe neuroepithelia
    Mo Weng
    Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Dev Neurobiol 72:1376-90. 2012
    ..Thus, changes in Notch signaling orchestrate a dynamic balance between maintenance and conversion of neuroepithelial cells during optic lobe neurogenesis...
  16. doi The balance between GMD and OFUT1 regulates Notch signaling pathway activity by modulating Notch stability
    Alvaro Glavic
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Spain
    Biol Res 44:25-34. 2011
    ..O-fucosyltransferase-1 (OFUT1) performs the first glycosylation step in this process, O-fucosylating various EGF repeats at the Notch ..
  17. pmc Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling
    Tetsuya Okajima
    Nagoya University Graduate School of Bioagricultural Sciences, Department of Applied Molecular Biosciences, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    BMC Biol 6:1. 2008
    O-fucosyltransferase1 (OFUT1) is a conserved ER protein essential for Notch signaling. OFUT1 glycosylates EGF domains, which can then be further modified by the N-acetylglucosaminyltransferase Fringe...
  18. ncbi Protein O-fucosyltransferase 2 adds O-fucose to thrombospondin type 1 repeats
    Yi Luo
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, New York 11794 5215, USA
    J Biol Chem 281:9393-9. 2006
    ..These results demonstrate that O-fucosyltransferase 2 is in fact a TSR-specific O-fucosyltransferase...
  19. doi Distinct levels of Notch activity for commitment and terminal differentiation of stem cells in the adult fly intestine
    Carolina N Perdigoto
    Institut Pasteur, Developmental Biology Department, F 75015 Paris, France
    Development 138:4585-95. 2011
    ..Our work suggests that a commitment requirement for high-level Notch activity safeguards the stem cells from loss through differentiation, revealing a novel role for the importance of Notch signaling levels in this system...
  20. doi O-linked N-acetylglucosamine is present on the extracellular domain of notch receptors
    Aiko Matsuura
    Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sciences, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    J Biol Chem 283:35486-95. 2008
    ..Thus, the finding of O-GlcNAc modification in extracellular environments predicts a distinct glycosylation process that might be associated with a novel regulatory mechanism for Notch receptor activity...
  21. doi Selenoprotein dSelK in Drosophila elevates release of Ca2+ from endoplasmic reticulum by upregulating expression of inositol 1,4,5-tris-phosphate receptor
    S B Ben
    School of Life Science, Liaoning University, Shenyang, China
    Biochemistry (Mosc) 76:1030-6. 2011
    ..These results indicate that dSelK in D. melanogaster is involved in regulating the release of Ca2+ from the ER to the cytosol and may play important roles in the signal transduction pathways involving Ca2+ mobilization...
  22. doi O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions
    Yuta Sakaidani
    Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa Ku, Nagoya 466 0065, Japan
    Nat Commun 2:583. 2011
    ..Thus, O-GlcNAcylation of secreted and membrane glycoproteins is a novel mediator of cell-cell or cell-matrix interactions at the cell surface...
  23. doi Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila
    Tomoko Yamakawa
    Department of Biological Science and Technology, Tokyo University of Science, Noda, Chiba, 278 8510 Japan
    Development 139:558-67. 2012
    ..Our results indicate that the ER plays a previously unrecognized role in N signaling and that this ER function depends on pcx activity...
  24. ncbi Organization and regulation of sex-specific thioredoxin encoding genes in the genus Drosophila
    Malin J Svensson
    UCMP, Umea University, SE 901 87, Umea, Sweden
    Dev Genes Evol 217:639-50. 2007
    ..In addition, we show that the 50-70 C terminal amino acids of TrxT constitute a hyper-variable domain, which could play a role in sexual conflict and male-female co-evolution...
  25. pmc The adhesion force of Notch with Delta and the rate of Notch signaling
    Francois Ahimou
    Department of Microbiology and Molecular Genetics, The University of Vermont, VT 05405, USA
    J Cell Biol 167:1217-29. 2004
    ..Reduced turnover or Delta pulling accelerate this loss. These data suggest that strong adhesion between Notch and Delta might serve as a booster for initiating Notch signaling at a high rate...
  26. ncbi EGF receptor signaling regulates pulses of cell delamination from the Drosophila ectoderm
    Véronique Brodu
    Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Dev Cell 7:885-95. 2004
    ..These findings illustrate how intercellular signaling and cell movements can generate multiple cycles of a cell behavior, despite individual cells experiencing only one cycle of receptor activation...
  27. ncbi Regulation of notch signaling activity
    Francois Schweisguth
    CNRS UMR 8542, Ecole Normale Superieure, Paris, France
    Curr Biol 14:R129-38. 2004
    ..Recent analyses of the cell biology of the Notch receptor have identified several fundamental mechanisms that contribute to regulate Notch signaling activity in space and time...
  28. ncbi Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase
    Y Wang
    Department of Pharmacokinetics and Metabolism, Genentech, Inc, South San Francisco, California 94080, USA
    J Biol Chem 276:40338-45. 2001
    ..The identification of the gene encoding protein O-fucosyltransferase I now makes possible mutational strategies to examine the functions of the unusual O-fucose post-translational modification...