Mtk

Summary

Gene Symbol: Mtk
Description: Metchnikowin
Alias: BcDNA:RH07954, CG8175, DIM 17, Dmel\CG8175, METCH, Metc, Metch, metch, metk, mtk, CG8175 gene product from transcript CG8175-RA, CG8175-PA, Mtk-PA, drosophila immune induced molecule 17, metchikowin, metchinkowin, metchnikovin, metchnikowin, metchnikowin A, metchnikowin B, metchnikowin precursor, metchnikowin-1, metchnikowin-2, metchnikowine
Species: fruit fly

Top Publications

  1. pmc Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection
    Iiro Taneli Helenius
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 106:18710-5. 2009
  2. ncbi REL1, a homologue of Drosophila dorsal, regulates toll antifungal immune pathway in the female mosquito Aedes aegypti
    Sang Woon Shin
    Department of Entomology and the Institute for Integrative Genome Biology, University of California, Riverside, California 92521, USA
    J Biol Chem 280:16499-507. 2005
  3. ncbi Cutting edge: the toll pathway is required for resistance to gram-positive bacterial infections in Drosophila
    Sophie Rutschmann
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    J Immunol 168:1542-6. 2002
  4. ncbi Requirement for a peptidoglycan recognition protein (PGRP) in Relish activation and antibacterial immune responses in Drosophila
    Kwang Min Choe
    Molecular Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Science 296:359-62. 2002
  5. doi Identification of a cis-regulatory element required for 20-hydroxyecdysone enhancement of antimicrobial peptide gene expression in Drosophila melanogaster
    Z Zhang
    Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, USA
    Insect Mol Biol 18:595-605. 2009
  6. ncbi Metchnikowin, a novel immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties
    E A Levashina
    Department of Genetics and Breeding, St Petersburg State University, Russia
    Eur J Biochem 233:694-700. 1995
  7. pmc Down-regulation of NF-kappaB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila
    Lark Kyun Kim
    Department of Biochemistry, National Creative Research Initiative Center for Genome Regulation, Yonsei University, Seoul, Korea
    PLoS Biol 5:e238. 2007
  8. ncbi Immunity regulatory DNAs share common organizational features in Drosophila
    Kate Senger
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 13:19-32. 2004
  9. ncbi Genetic basis of natural variation in D. melanogaster antibacterial immunity
    Brian P Lazzaro
    Department of Entomology, 4138 Comstock Hall, Cornell University, Ithaca, NY 14853, USA
    Science 303:1873-6. 2004
  10. doi FOXO-dependent regulation of innate immune homeostasis
    Thomas Becker
    Development, Genetics and Molecular Physiology Unit, LIMES Institute, University of Bonn, Carl Troll Strasse 31, D 53115 Bonn, Germany
    Nature 463:369-73. 2010

Research Grants

  1. Evolutionary Genomics of Anti-Malaria Genes in Mosquito
    Brian P Lazzaro; Fiscal Year: 2010
  2. The Role of Peroxiredoxins in Immunity and Aging
    SVETLANA RADYUK; Fiscal Year: 2006
  3. REGULATION OF IMMUNE RESPONSE AND CELL GROWTH
    DEBORAH KIMBRELL; Fiscal Year: 2001

Scientific Experts

  • BRIAN LAZZARO
  • H Anbutsu
  • Kenneth M Fedorka
  • Svetlana N Radyuk
  • S Fellous
  • Yoshimasa Yagi
  • Sarah M Short
  • Timothy B Sackton
  • DEBORAH KIMBRELL
  • François Leulier
  • Katie E Marshall
  • Catherine A Brennan
  • J A Hoffmann
  • Seiji Tsuzuki
  • Kate Senger
  • Elissa A Hallem
  • J M Reichhart
  • B Lemaitre
  • Jennifer M Peña
  • Lisa J Sudmeier
  • Shanming Ji
  • Chun Taek Oh
  • Hitoshi Matsumoto
  • Yosuke Seto
  • Matthew S Thimgan
  • J C Castillo
  • Yoichi Hayakawa
  • E A Levashina
  • Ki Moon Seong
  • Zhipeng Wang
  • Lorri R Marek
  • Gayle Overend
  • Rebecca I Clark
  • Xiang Jun Rao
  • D Ferrandon
  • Julian A T Dow
  • Pablo Cabrero
  • Huiwen W Zhao
  • Ahmed Abdelsadik
  • Keetae Kim
  • Thomas Becker
  • S Rutschmann
  • Wade E Winterhalter
  • Aurélien Lauwers
  • Alexandre Costa
  • Iiro Taneli Helenius
  • Vasanthi Avadhanula
  • Erin M Hill-Burns
  • M Hedengren
  • Z Zhang
  • Konstantinos Stergiopoulos
  • Neal Silverman
  • Anni Kleino
  • Thomas Flatt
  • Ji Hwan Ryu
  • Georgios Chamilos
  • C W Tsai
  • Darien E Reed
  • Huaping Tang
  • Jodell E Linder
  • Katherine Taylor
  • Lark Kyun Kim
  • Richard R Copley
  • Y Lu
  • L P Wu
  • Joseph R Delaney
  • P Tzou
  • Viola Gesellchen
  • Shin Young Park
  • Dan Hultmark
  • Jing Peng
  • Robert A Zambon
  • Sang Woon Shin
  • Hsiling Chiu
  • M S Dushay
  • Kathryn V Anderson
  • Svenja Stoven
  • Caroline R Craig
  • Mayra A Carrillo
  • Steven P Howard
  • Sai Suma Samudrala
  • Barry Ganetzky
  • Eui Jae Sung
  • Qinmiao Sun
  • Servane Tauszig-Delamasure
  • Sophie Rutschmann
  • Ming Sun
  • Hirotoshi Tanaka
  • Dahua Chen
  • Shunsuke Furihata

Detail Information

Publications86

  1. pmc Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection
    Iiro Taneli Helenius
    Department of Biochemistry, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 106:18710-5. 2009
    ....
  2. ncbi REL1, a homologue of Drosophila dorsal, regulates toll antifungal immune pathway in the female mosquito Aedes aegypti
    Sang Woon Shin
    Department of Entomology and the Institute for Integrative Genome Biology, University of California, Riverside, California 92521, USA
    J Biol Chem 280:16499-507. 2005
    ..bassiana. These results indicate that the homologue of Dl, but not that of Dif, is a key regulator of the Toll antifungal immune pathway in A. aegypti female mosquitoes...
  3. ncbi Cutting edge: the toll pathway is required for resistance to gram-positive bacterial infections in Drosophila
    Sophie Rutschmann
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    J Immunol 168:1542-6. 2002
    ..In this study, we report that the Toll pathway, but not that of immune deficiency, is required for resistance to other Gram-positive bacteria and that this response does not involve Defensin...
  4. ncbi Requirement for a peptidoglycan recognition protein (PGRP) in Relish activation and antibacterial immune responses in Drosophila
    Kwang Min Choe
    Molecular Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Science 296:359-62. 2002
    ....
  5. doi Identification of a cis-regulatory element required for 20-hydroxyecdysone enhancement of antimicrobial peptide gene expression in Drosophila melanogaster
    Z Zhang
    Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, USA
    Insect Mol Biol 18:595-605. 2009
    ..peptidoglycan-induced expression of four other antimicrobial peptide (AMP) genes--drosomycin, attacin-A, metchnikowin and cecropin A1...
  6. ncbi Metchnikowin, a novel immune-inducible proline-rich peptide from Drosophila with antibacterial and antifungal properties
    E A Levashina
    Department of Genetics and Breeding, St Petersburg State University, Russia
    Eur J Biochem 233:694-700. 1995
    ..The novel peptide, which we propose to name metchnikowin, is a member of a family of proline-rich peptides, and we discuss the possible evolutionary relationships ..
  7. pmc Down-regulation of NF-kappaB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila
    Lark Kyun Kim
    Department of Biochemistry, National Creative Research Initiative Center for Genome Regulation, Yonsei University, Seoul, Korea
    PLoS Biol 5:e238. 2007
    ..We conclude that an inhibitory effect of AP-1 and STAT on NF-kappaB is required for properly balanced immune responses and appears to be evolutionarily conserved...
  8. ncbi Immunity regulatory DNAs share common organizational features in Drosophila
    Kate Senger
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 13:19-32. 2004
    ..Aspects of this "regulatory code" are essential for the immune response. These results suggest that immunity regulatory DNAs contain constrained organizational features, which may be a general property of eukaryotic enhancers...
  9. ncbi Genetic basis of natural variation in D. melanogaster antibacterial immunity
    Brian P Lazzaro
    Department of Entomology, 4138 Comstock Hall, Cornell University, Ithaca, NY 14853, USA
    Science 303:1873-6. 2004
    ..Variation in these genes, therefore, seems to drive variability in immunocompetence among wild Drosophila...
  10. doi FOXO-dependent regulation of innate immune homeostasis
    Thomas Becker
    Development, Genetics and Molecular Physiology Unit, LIMES Institute, University of Bonn, Carl Troll Strasse 31, D 53115 Bonn, Germany
    Nature 463:369-73. 2010
    ..The sparse production of AMPs in epithelial tissues in response to FOXO may help modulating the defence reaction without harming the host tissues, in particular when animals are suffering from energy shortage or stress...
  11. ncbi Drosophila MyD88 is required for the response to fungal and Gram-positive bacterial infections
    Servane Tauszig-Delamasure
    UPR9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, 15, rue René Descartes, 67000 Strasbourg, France
    Nat Immunol 3:91-7. 2002
    ..Phenotypic comparison of DmMyD88-mutant flies and MyD88-deficient mice showed essential differences in the control of Gram-negative infection in insects and mammals...
  12. pmc The Toll pathway is important for an antiviral response in Drosophila
    Robert A Zambon
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    Proc Natl Acad Sci U S A 102:7257-62. 2005
    ..Additionally, our results demonstrate the validity of using a genetic approach to identify genes and pathways used in viral innate immune responses in Drosophila...
  13. pmc Phosphoinositide binding by the Toll adaptor dMyD88 controls antibacterial responses in Drosophila
    Lorri R Marek
    Division of Gasteroenterology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Immunity 36:612-22. 2012
    ..We therefore propose that dMyD88 is the functional homolog of TIRAP and that both proteins function as sorting adaptors to recruit downstream signaling adaptors to activated receptors...
  14. pmc A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response
    Vasanthi Avadhanula
    Department of Biology, Indiana University, Bloomington, IN, USA
    PLoS Pathog 5:e1000582. 2009
    ..These findings show that the Imd pathway mediates an antiviral response to Sindbis virus replication. To our knowledge, this is the first demonstration of an antiviral role for the Imd pathway in insects...
  15. ncbi Nematodes, bacteria, and flies: a tripartite model for nematode parasitism
    Elissa A Hallem
    Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Curr Biol 17:898-904. 2007
    ....
  16. pmc Cooperative control of Drosophila immune responses by the JNK and NF-kappaB signaling pathways
    Joseph R Delaney
    Brookdale Department of Developmental, Cell and Molecular Biology, The Mount Sinai School of Medicine, New York, NY, USA
    EMBO J 25:3068-77. 2006
    ..Our data indicate that dTAK1 is not required for Relish activation, but instead is required in JNK signaling for antimicrobial peptide gene expression...
  17. pmc The Imd pathway is involved in antiviral immune responses in Drosophila
    Alexandre Costa
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    PLoS ONE 4:e7436. 2009
    ..Our data show that antiviral innate immune responses in flies infected with CrPV depend upon hemocytes and signaling through the Imd pathway...
  18. ncbi Psidin is required in Drosophila blood cells for both phagocytic degradation and immune activation of the fat body
    Catherine A Brennan
    Department of Microbiology and Immunology, Stanford University School of Medicine, 299 Campus Drive, Stanford, California 94305, USA
    Curr Biol 17:67-72. 2007
    ..These data establish a role for the phagocytic blood cells of Drosophila in detection of infection and activation of the humoral immune response...
  19. pmc Drosophila melanogaster mounts a unique immune response to the Rhabdovirus sigma virus
    C W Tsai
    Department of Entomology, The Ohio State University OARDC, Wooster, Ohio 44691, USA
    Appl Environ Microbiol 74:3251-6. 2008
    ..SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections...
  20. pmc A serpin that regulates immune melanization in the respiratory system of Drosophila
    Huaping Tang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Cell 15:617-26. 2008
    ..Such signaling between local and systemic immune responses could represent an alarm mechanism that prepares the host in case a pathogen breaches epithelial defenses to invade internal tissues...
  21. pmc Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms
    B Lemaitre
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 94:14614-9. 1997
    ..This response is mediated through the selective activation of the Toll pathway...
  22. pmc The Drosophila caspase Dredd is required to resist gram-negative bacterial infection
    F Leulier
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, France
    EMBO Rep 1:353-8. 2000
    ....
  23. pmc The antibacterial arm of the drosophila innate immune response requires an IkappaB kinase
    Y Lu
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Genes Dev 15:104-10. 2001
    ..The ird5 gene encodes a Drosophila homolog of mammalian IkappaB kinases (IKKs). The ird5 phenotype and sequence suggest that the gene is specifically required for the activation of Relish, a Drosophila NF-kappaB family member...
  24. ncbi Pirk is a negative regulator of the Drosophila Imd pathway
    Anni Kleino
    Institute of Medical Technology, University of Tampere, Finland
    J Immunol 180:5413-22. 2008
    ..Furthermore, pirk-overexpressing flies were more susceptible to Gram-negative bacterial infection than wild-type flies. We conclude that Pirk is a negative regulator of the Imd pathway...
  25. ncbi Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin
    E A Levashina
    Réponse Immunitaire et Développement chez les Insectes, UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg, 67000, France
    J Mol Biol 278:515-27. 1998
    b>Metchnikowin is a recently discovered proline-rich peptide from Drosophila with antibacterial and antifungal properties. Like most other antimicrobial peptides from insects, its expression is immune-inducible...
  26. ncbi Role of Drosophila IKK gamma in a toll-independent antibacterial immune response
    S Rutschmann
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15, rue R Descartes, F67084 Strasbourg, France
    Nat Immunol 1:342-7. 2000
    ..Thus, in contrast to the vertebrate inflammatory response, IKK gamma is required for the activation of only one immune signaling pathway in Drosophila...
  27. ncbi The Rel protein DIF mediates the antifungal but not the antibacterial host defense in Drosophila
    S Rutschmann
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France
    Immunity 12:569-80. 2000
    ..We discuss in this light the possibility that NF-KB1/p50 might be required more specifically in the innate immune response against gram-positive bacteria in mammals...
  28. pmc Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study
    S Uttenweiler-Joseph
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, 15, rue René Descartes, 67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 95:11342-7. 1998
    ..Finally, molecular cloning and Northern blot analyses revealed that one of the DIMs is produced as a prepropeptide and is inducible on a bacterial challenge...
  29. pmc Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes
    A Braun
    Unité Propre de Recherche 9022, Réponse Immunitaire et Développement chez les Insectes, Institut de Biologie Moléculaire et Cellulaire 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    Proc Natl Acad Sci U S A 95:14337-42. 1998
    ..By working in synergy, they provide Drosophila a highly effective defense against injury and/or infection...
  30. pmc Drosophila melanogaster as a model host to dissect the immunopathogenesis of zygomycosis
    Georgios Chamilos
    Department of Infectious Diseases, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:9367-72. 2008
    ..Our results provide insights into the factors that mediate host-pathogen interactions in zygomycosis and establish D. melanogaster as a promising model to study this important mycosis...
  31. pmc Hormonal regulation of the humoral innate immune response in Drosophila melanogaster
    Thomas Flatt
    Division of Biology and Medicine, Department of Ecology and Evolutionary Biology, Brown University, Providence, RI 02912, USA
    J Exp Biol 211:2712-24. 2008
    ..Our results suggest that 20E and JH play major roles in the regulation of gene expression in response to immune challenge...
  32. ncbi Drosophila immune deficiency (IMD) is a death domain protein that activates antibacterial defense and can promote apoptosis
    P Georgel
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France
    Dev Cell 1:503-14. 2001
    ..We also show that imd is involved in the apoptotic response to UV irradiation. These data raise the possibility that antibacterial response and apoptosis share common control elements in Drosophila...
  33. ncbi Drosophila sex-peptide stimulates female innate immune system after mating via the Toll and Imd pathways
    Jing Peng
    Zoological Institute, University of Zurich Irchel, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Curr Biol 15:1690-4. 2005
    ..Here, we show that AMP genes are differentially expressed in mated females. Metchnikowin (Mtk) expression is strongly stimulated in the first 6 hr after mating...
  34. ncbi Relish, a central factor in the control of humoral but not cellular immunity in Drosophila
    M Hedengren
    Umeå Center for Molecular Pathogenesis, Umea University, Sweden
    Mol Cell 4:827-37. 1999
    ..Our results illustrate the importance of the humoral response in Drosophila immunity and demonstrate that Relish plays a key role in this response...
  35. ncbi Tissue-specific inducible expression of antimicrobial peptide genes in Drosophila surface epithelia
    P Tzou
    Centre de Génétique Moléculaire du CNRS, 91198 Gif sur Yvette, France
    Immunity 13:737-48. 2000
    ....
  36. pmc Mutations in the Drosophila dTAK1 gene reveal a conserved function for MAPKKKs in the control of rel/NF-kappaB-dependent innate immune responses
    S Vidal
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Genes Dev 15:1900-12. 2001
    ..Our genetic demonstration of dTAK1's role in the regulation of Drosophila antimicrobial peptide gene expression suggests an evolutionary conserved role for TAK1 in the activation of Rel/NF-kappaB-mediated host defense reactions...
  37. pmc DEAF-1 regulates immunity gene expression in Drosophila
    Darien E Reed
    Helen Diller Family Comprehensive Cancer Center and Cancer Research Institute, University of California, San Francisco, CA 94115 0128, USA
    Proc Natl Acad Sci U S A 105:8351-6. 2008
    ..immune response and is specifically important for the induction of two genes encoding antimicrobial peptides, Metchnikowin (Mtk) and Drosomycin (Drs)...
  38. pmc Post-mating disparity between potential and realized immune response in Drosophila melanogaster
    Kenneth M Fedorka
    Department of Biology, University of Central Florida, Orlando, FL 32816, USA
    Proc Biol Sci 274:1211-7. 2007
    ..Thus, estimates of immunity based on gene expression do not appear to reflect an actual ability to defend against pathogens in the hours following copulation. We discuss the possible mechanisms that may account for this pattern...
  39. pmc Persistent Activation of the Innate Immune Response in Adult Drosophila Following Radiation Exposure During Larval Development
    Lisa J Sudmeier
    Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706
    G3 (Bethesda) 5:2299-306. 2015
    ..These studies may facilitate the development of strategies to reduce the deleterious side effects of CRT. ..
  40. pmc Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress
    Seiji Tsuzuki
    Department of Applied Biological Sciences, Saga University, Honjo
    Sci Rep 2:210. 2012
    ..These results indicate that the Drosophila GBP signaling pathway mediates acute innate immune reactions under various stresses, regardless of whether they are infectious or non-infectious...
  41. pmc Directed expression of the HIV-1 accessory protein Vpu in Drosophila fat-body cells inhibits Toll-dependent immune responses
    François Leulier
    Centre de Génétique Moléculaire Centre National de la Recherche Scientifique, Batiment 26, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    EMBO Rep 4:976-81. 2003
    ....
  42. doi Immune gene transcription in Drosophila adult flies infected by entomopathogenic nematodes and their mutualistic bacteria
    J C Castillo
    Insect Infection and Immunity Lab, Department of Biological Sciences, Institute for Biomedical Sciences, Columbian College of Arts and Sciences, The George Washington University, Washington, DC 20052, USA
    J Insect Physiol 59:179-85. 2013
    ..These results suggest that Heterorhabditis nematodes and their associated Photorhabdus bacteria employ distinct strategies to evade the Drosophila immune response and establish infection...
  43. pmc Cross-translational studies in human and Drosophila identify markers of sleep loss
    Matthew S Thimgan
    Department of Anatomy and Physiology, Washington University School of Medicine, St Louis, Missouri, United States of America
    PLoS ONE 8:e61016. 2013
    ..These cross-translational data indicate that fly and human discovery experiments are mutually reinforcing and can be used interchangeably to identify candidate biomarkers of sleep loss...
  44. doi Drosophila melanogaster model for Mycobacterium abscessus infection
    Chun Taek Oh
    Drug Biology, Institut Pasteur Korea, Seongnam si, Gyeonggi Do, Republic of Korea
    Microbes Infect 15:788-95. 2013
    ..To the best of our knowledge, this is the first report that mycobacteria induce the production of antimicrobial peptides in D. melanogaster. ..
  45. pmc Extensive differences in antifungal immune response in two Drosophila species revealed by comparative transcriptome analysis
    Yosuke Seto
    Department of Biological Sciences, Tokyo Metropolitan University, 1 1 Minami Osawa, Hachioji shi, Tokyo 192 0397, Japan
    Int J Genomics 2013:542139. 2013
    ..melanogaster and D. virilis: the genes encoding antifungal peptides, Drosomycin and Metchnikowin, were highly expressed in D...
  46. pmc Cell-surface localization of Pellino antagonizes Toll-mediated innate immune signalling by controlling MyD88 turnover in Drosophila
    Shanming Ji
    1 State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 2 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 3
    Nat Commun 5:3458. 2014
    ..Collectively, our study uncovers a mechanism by which a feedback regulatory loop involving MyD88 and Pellino controls Toll-mediated signalling, thereby maintaining homeostasis of host innate immunity. ..
  47. doi Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP
    Seiji Tsuzuki
    1 Department of Applied Biological Sciences, Saga University, Saga 840 8502, Japan 2
    Nat Commun 5:4628. 2014
    ..Thus, we describe mechanisms to initiate immune programs that are either humoral or cellular in nature, but not both; such immunophysiological polarization may minimize homeostatic imbalance during infection. ..
  48. pmc Variation in the susceptibility of Drosophila to different entomopathogenic nematodes
    Jennifer M Peña
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, USA
    Infect Immun 83:1130-8. 2015
    ..Our results establish a powerful model system for understanding mechanisms of host-parasite interactions and the insect immune response to parasitic nematode infection. ..
  49. doi Evasion, suppression and tolerance of Drosophila innate immunity by a male-killing Spiroplasma endosymbiont
    H Anbutsu
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Insect Mol Biol 19:481-8. 2010
    ..These findings suggest that not only immune evasion but also immune suppression and tolerance are involved in the establishment and maintenance of the insect-microbe symbiotic association...
  50. pmc Chronic activation of the epithelial immune system of the fruit fly's salivary glands has a negative effect on organismal growth and induces a peculiar set of target genes
    Ahmed Abdelsadik
    Christian Albrechts University of Kiel, Zoophysiology, Kiel, Germany
    BMC Genomics 11:265. 2010
    ..Our major goal was to elucidate if the fly's salivary glands are able to mount an immune response and what the major characteristics of this immune response are...
  51. pmc Repeated stress exposure results in a survival-reproduction trade-off in Drosophila melanogaster
    Katie E Marshall
    Department of Biology, The University of Western Ontario, London, Ontario, Canada
    Proc Biol Sci 277:963-9. 2010
    ..Given that many environments are not static, this trade-off indicates that investigating the effects of repeated stress exposure is important for understanding and predicting physiological responses in the wild...
  52. pmc Peroxiredoxin 5 modulates immune response in Drosophila
    Svetlana N Radyuk
    Department of Biological Sciences, Southern Methodist University, 6501 Airline Rd, Room 113, Dallas, TX 75275, USA
    Biochim Biophys Acta 1800:1153-63. 2010
    ..Previously, we reported on the potent antioxidant function of Drosophila peroxiredoxin 5 (dPrx5). Studies with mammalian and human cells suggest that peroxiredoxins can modulate immune-related signaling...
  53. doi Larval food quality affects adult (but not larval) immune gene expression independent of effects on general condition
    Simon Fellous
    Department of Entomology, Cornell University, Ithaca, NY 14853, USA
    Mol Ecol 19:1462-8. 2010
    ..We conclude that the nutritional environment of insect larvae can affect adult immunity by influencing plastic allocation of resources. These influences are less predictable than constraints linked to general condition would be...
  54. pmc Experimental selection for Drosophila survival in extremely high O2 environments
    Huiwen W Zhao
    Division of Respiratory Medicine, Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 5:e11701. 2010
    ..Thus our study provides a molecular basis for understanding the mechanisms of hyperoxia tolerance...
  55. doi Enhancement of stress resistances and downregulation of Imd pathway by lower developmental temperature in Drosophila melanogaster
    Keetae Kim
    Department of Cell Biology and Molecular Medicine, UMDNJ New Jersey Medical School, Newark, NJ 07103, United States
    Exp Gerontol 45:984-7. 2010
    ..We also showed that development at a lower temperature represented by 18°C significantly downregulates anti-microbial peptide genes, AttA and DptB, of Imd pathway...
  56. pmc Functional analysis of Toll-related genes in Drosophila
    Yoshimasa Yagi
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Dev Growth Differ 52:771-83. 2010
    ..Overall our results suggest that some of the Toll-related proteins, such as 18W, Toll-7 and Toll-8, may have redundant functions in regulating developmental processes...
  57. doi The receptor guanylate cyclase Gyc76C and a peptide ligand, NPLP1-VQQ, modulate the innate immune IMD pathway in response to salt stress
    Gayle Overend
    Institute of Molecular, Cell and Systems Biology, University of Glasgow, Glasgow, Scotland, UK
    Peptides 34:209-18. 2012
    ..Thus, the principal-cell localized NPLP1-VQQ/Gyc76C cGMP pathway acts to signal environmental (salt) stress to the whole organism...
  58. pmc Multiple TGF-β superfamily signals modulate the adult Drosophila immune response
    Rebecca I Clark
    Centre for the Molecular and Cellular Biology of Inflammation and Peter Gorer Department of Immunobiology, King s College London School of Medicine, London SE1 1UL, UK
    Curr Biol 21:1672-7. 2011
    ..Together, these data imply a model in which the bone morphogenetic protein (BMP) dpp is an important inhibitor of inflammation following sterile injury whereas the activin-like dawdle determines the nature of the induced immune response...
  59. pmc Functional conservation of Rel binding sites in drosophilid genomes
    Richard R Copley
    Wellcome Trust Centre for Human Genetics, Oxford University, Oxford OX3 7BN, United Kingdom
    Genome Res 17:1327-35. 2007
    ..We discuss how two types of conservation may contribute to the stabilization and optimization of a functional gene regulatory code in evolution...
  60. pmc Manduca sexta moricin promoter elements can increase promoter activities of Drosophila melanogaster antimicrobial peptide genes
    Xiang Jun Rao
    Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
    Insect Biochem Mol Biol 41:982-92. 2011
    ..These findings revealed an approach of engineering AMP genes with enhanced activities, which may lead to broad applications...
  61. ncbi Low-dose radiation induces Drosophila innate immunity through Toll pathway activation
    Ki Moon Seong
    Division of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co, Ltd, Seoul, Korea 132 703
    J Radiat Res 53:242-9. 2012
    ..These results indicate that innate immunity is activated after low-dose irradiation through Toll signaling pathway in Drosophila...
  62. ncbi Drosophila melanogaster is susceptible to Vibrio cholerae infection
    Shin Young Park
    Department of Life Science, Sogang University, Seoul 121 742, Korea
    Mol Cells 20:409-15. 2005
    ..cholerae infection compared to V. vulnificus infection. Ectopic expression of Attacin A and Metchnikowin enhanced the survival of D. melanogaster upon V. cholerae infection...
  63. pmc The Drosophila protein mustard tailors the innate immune response activated by the immune deficiency pathway
    Zhipeng Wang
    Division of Infectious Diseases, Children s Hospital Boston, Boston, MA 02115, USA
    J Immunol 188:3993-4000. 2012
    ..Mtd homologs have been implicated in resistance to oxidative stress. However, to our knowledge this is the first evidence that Mtd or its homologs alter the output of an innate immunity signaling cascade from within the nucleus...
  64. pmc Female Drosophila melanogaster suffer reduced defense against infection due to seminal fluid components
    Sarah M Short
    Field of Genetics and Development, Cornell University Ithaca, NY 14853, USA
    J Insect Physiol 58:1192-201. 2012
    ....
  65. pmc The effects of temperature on host-pathogen interactions in D. melanogaster: who benefits?
    Jodell E Linder
    Department of Genetics, University of Georgia, Athens, GA 30602, USA
    J Insect Physiol 54:297-308. 2008
    ..The approach described here allows us to determine whether this effect is due primarily to temperature-specific effects on the host or on its pathogen. These results suggest that both may be important...
  66. ncbi dUbc9 negatively regulates the Toll-NF-kappa B pathways in larval hematopoiesis and drosomycin activation in Drosophila
    Hsiling Chiu
    Department of Functional Genomics, Novartis Institutes for BioMedical Research, 100 Technology Square Bldg 601 Rm 6404, Cambridge, MA 02139, USA
    Dev Biol 288:60-72. 2005
    ..dUbc9-mediated drosomycin expression requires Dorsal and Dif. Together, our results support a role for dUbc9 in the negative regulation of the Drosophila NF-kappaB signaling pathways in larval hematopoiesis and humoral immunity...
  67. ncbi Antimicrobial peptide defense in Drosophila
    M Meister
    UPR 9022, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    Bioessays 19:1019-26. 1997
    ..Recent data on the molecular mechanisms underlying recognition of non-self are also discussed in this review...
  68. ncbi Phylogenetic perspectives in innate immunity
    J A Hoffmann
    Institute of Molecular and Cellular Biology, CNRS, Strasbourg, 67084, France
    Science 284:1313-8. 1999
    ..In addition to its role in the early phase of defense, innate immunity in mammals appears to play a key role in stimulating the subsequent, clonal response of adaptive immunity...
  69. ncbi Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila
    E A Levashina
    UPR 9022 CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg 67084, France
    Science 285:1917-9. 1999
    ..Hence, Spn43Ac negatively regulates the Toll signaling pathway, and Toll does not function as a pattern recognition receptor in the Drosophila host defense...
  70. ncbi Two attacin antibacterial genes of Drosophila melanogaster
    M S Dushay
    Department of Biological Sciences, University of Notre Dame, POB 369, Notre Dame, IN 46556, USA
    Gene 246:49-57. 2000
    ..We present a comparison of the promoter regions of the two attacin genes to those cloned from other antimicrobial peptide genes to assist a better understanding of how antimicrobial genes are differentially regulated...
  71. ncbi Expression and evolution of the Drosophila attacin/diptericin gene family
    M Hedengren
    Umea Centre for Molecular Pathogenesis, Umea University, Umea, S 901 87, Sweden
    Biochem Biophys Res Commun 279:574-81. 2000
    ..This induction is reduced in imd mutants, and unexpectedly also in Tl(-) mutants. The 18w mutation particularly affects the induction of AttC, which may be a useful marker for 18w signaling...
  72. ncbi Tehao functions in the Toll pathway in Drosophila melanogaster: possible roles in development and innate immunity
    C Luo
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA
    Insect Mol Biol 10:457-64. 2001
    ..transiently transfected cells, Tehao activated both Dorsal and the transcription of endogenous drosomycin and metchnikowin genes...
  73. pmc Critical evaluation of the role of the Toll-like receptor 18-Wheeler in the host defense of Drosophila
    Petros Ligoxygakis
    Institut de Biologie Moléculaire and Cellulaire, Strasbourg Cedex, France
    EMBO Rep 3:666-73. 2002
    ..18-Wheeler does not qualify as a pattern recognition receptor of Gram-negative bacteria...
  74. pmc A Drosophila p38 orthologue is required for environmental stress responses
    Caroline R Craig
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 S Euclid Avenue, Campus Box 8103, Saint Louis, Missouri 63110, USA
    EMBO Rep 5:1058-63. 2004
    ..These phenotypes only partially overlap those caused by mutations in D-MEKK1 and dTAK1, suggesting that the D-p38a gene is required to mediate some, but not all, of the functions ascribed to p38 signalling...
  75. pmc An RNA interference screen identifies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila
    Viola Gesellchen
    German Cancer Research Center DKFZ, Boveri Group Signaling and Functional Genomics, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
    EMBO Rep 6:979-84. 2005
    ..Our results indicate that IAP gene family members may have acquired other functions, such as the regulation of the tumour necrosis factor-like IMD pathway during innate immune responses...
  76. pmc Genetic variation in Drosophila melanogaster resistance to infection: a comparison across bacteria
    Brian P Lazzaro
    Department of Entomology, Field of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York 14853, USA
    Genetics 174:1539-54. 2006
    ..lactis and E. faecalis, respectively, most of the molecular polymorphisms tested explain <10% of the total variance in bacterial load sustained after infection...
  77. pmc The Drosophila inhibitor of apoptosis protein DIAP2 functions in innate immunity and is essential to resist gram-negative bacterial infection
    François Leulier
    The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, London SW3 6JB, UK
    Mol Cell Biol 26:7821-31. 2006
    ..Taken together, our genetic data identify DIAP2 as an essential component of the Imd signaling cascade, protecting the organism from infiltrating microbes...
  78. pmc GATA factors participate in tissue-specific immune responses in Drosophila larvae
    Kate Senger
    Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, Center for Integrative Genomics, University of California, Berkeley, CA 94720 3204, USA
    Proc Natl Acad Sci U S A 103:15957-62. 2006
    ..Regulatory regions for antimicrobial genes Diptericin and Metchnikowin require GATA sites for activation in the midgut, where Grain (dGATAc), dGATAd, and dGATAe are expressed in ..
  79. doi Innate immune homeostasis by the homeobox gene caudal and commensal-gut mutualism in Drosophila
    Ji Hwan Ryu
    Division of Molecular Life Science, Ewha Woman s University and National Creative Research Initiative Center for Symbiosystem, Seoul 120 750, South Korea
    Science 319:777-82. 2008
    ..These results reveal that a specific genetic deficiency within a host can profoundly influence the gut commensal microbial community and host physiology...
  80. ncbi Host immune response and differential survival of the sexes in Drosophila
    Katherine Taylor
    Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616, USA
    Fly (Austin) 1:197-204. 2007
    ..We also found that antimicrobial protein gene mRNA levels for Drosomycin and Metchnikowin showed both similarities and differences between the sexes...
  81. pmc Salty dog, an SLC5 symporter, modulates Drosophila response to salt stress
    Konstantinos Stergiopoulos
    Integrative and Systems Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Physiol Genomics 37:1-11. 2009
    ..Accordingly, CG2196 was renamed salty dog (salt)...
  82. pmc Sex-specific variation in the emphasis, inducibility and timing of the post-mating immune response in Drosophila melanogaster
    Wade E Winterhalter
    Department of Biology, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA
    Proc Biol Sci 276:1109-17. 2009
    ..Our results suggest that variation in the effectiveness of the immune response between the sexes may be driven by differences in emphasis rather than overall investment...
  83. pmc Post-transcriptional Regulation of Genes Encoding Anti-microbial Peptides in Drosophila
    Aurélien Lauwers
    Laboratoire de Biologie Moléculaire du Gène, Faculte des Sciences, Universite Libre de Bruxelles, 12 rue des Prof Jeener et Brachet, 6041 Gosselies, Belgium
    J Biol Chem 284:8973-83. 2009
    ....
  84. pmc Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing
    Timothy B Sackton
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    BMC Genomics 10:259. 2009
    ..However, limited functional annotation in non-model systems has hindered understanding of evolutionary novelties in the insect innate immune system...
  85. pmc X-linked variation in immune response in Drosophila melanogaster
    Erin M Hill-Burns
    Field of Genetics and Development, Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Genetics 183:1477-91. 2009
    ..Many of the associations act in a sex-specific or sexually antagonistic manner, supporting the theory that with the selective pressures facing genes on the X chromosome, sexually antagonistic variation may be more easily maintained...
  86. pmc Drosophila immunity: genes on the third chromosome required for the response to bacterial infection
    L P Wu
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, Maryland 20742, USA
    Genetics 159:189-99. 2001
    ..These genetic studies should provide a basis for a comprehensive understanding of the genetic control of immune responses in Drosophila...

Research Grants3

  1. Evolutionary Genomics of Anti-Malaria Genes in Mosquito
    Brian P Lazzaro; Fiscal Year: 2010
    ..Evolutionary patterns in mosquito defense genes will be used to identify proteins that coevolve with malaria parasites. This work may suggest genetic targets that could be manipulated to limit malaria transmission. ..
  2. The Role of Peroxiredoxins in Immunity and Aging
    SVETLANA RADYUK; Fiscal Year: 2006
    ..This will set the stage for a more thorough investigation of the molecular underpinnings of innate immunity during aging. ..
  3. REGULATION OF IMMUNE RESPONSE AND CELL GROWTH
    DEBORAH KIMBRELL; Fiscal Year: 2001
    ..abstract_text> ..