Lam

Summary

Gene Symbol: Lam
Description: Lamin
Alias: 2459, 74/76, CG6944, DM[[O]], D[[m0]], Dm(0), Dm0, Dm1, Dm2, DmLamin, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], Dmel\CG6944, Dmo, LAM, Lam Dm[[0]], Lam(Dm0), LamDm, LamDm0, LamDm[[0]], LamDm[[o]], Lam[[Dm0]], Lamin B, Lamin Dm[[0]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, lamDm0, lamDm[[0]], lamin, lamin B, lamin Dm0, lamin Dm[[0]], lamin-B, misg, nlam, CG6944 gene product from transcript CG6944-RD, B-type lamin, CG6944-PA, CG6944-PB, CG6944-PC, CG6944-PD, Lam-PA, Lam-PB, Lam-PC, Lam-PD, Lamin-B, LaminDm, LaminDm0, alpha-Lamin, intermediate filament-like lamina, lamin, lamin A, lamin B, lamin D, lamin D[[0]], lamin D[[m0]], lamin D[[m]]0, lamin D[[o]], lamin Dm, lamin Dm0, lamin Dm[[0]], lamin Dmo, lethal(2)25Ec, misguided, misrouted, n-lamin, nuclear lamin
Species: fruit fly

Top Publications

  1. pmc A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene
    G Gregory Neely
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Cell 143:628-38. 2010
  2. ncbi Phosphorylation of the major Drosophila lamin in vivo: site identification during both M-phase (meiosis) and interphase by electrospray ionization tandem mass spectrometry
    U Schneider
    Department of Biochemistry, Biozentrum of the University of Basel, Switzerland
    Biochemistry 38:4620-32. 1999
  3. pmc Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene
    Sandra R Schulze
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Genetics 171:185-96. 2005
  4. ncbi Caspases function in autophagic programmed cell death in Drosophila
    Damali N Martin
    Center for Biosystems Research, University of Maryland Biotechnology Institute, and Department of Cell Biology, University of Maryland, College Park, Maryland 20742, USA
    Development 131:275-84. 2004
  5. pmc Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila
    Deborah L Berry
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    Cell 131:1137-48. 2007
  6. pmc Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations
    J C Fung
    Graduate Group in Biophysics, University of California, San Francisco, California 94143 0554, USA
    J Cell Biol 141:5-20. 1998
  7. ncbi Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos
    M R Paddy
    Center for Structural Biology and Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610 0235, USA
    J Cell Sci 109:591-607. 1996
  8. ncbi Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation
    Joachim Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Chromosoma 111:13-21. 2002
  9. pmc drICE is an essential caspase required for apoptotic activity in Drosophila cells
    A G Fraser
    Biochemistry of the Cell Nucleus Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 16:6192-9. 1997
  10. pmc Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract
    N Ulitzur
    Department of Genetics, Hebrew University of Jerusalem, Israel
    J Cell Biol 119:17-25. 1992

Research Grants

Scientific Experts

Detail Information

Publications119 found, 100 shown here

  1. pmc A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene
    G Gregory Neely
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr Bohr Gasse 3 5, A 1030 Vienna, Austria
    Cell 143:628-38. 2010
    ..Intriguingly, in α2δ3 mutant mice, thermal pain and tactile stimulation triggered strong cross-activation, or synesthesia, of brain regions involved in vision, olfaction, and hearing...
  2. ncbi Phosphorylation of the major Drosophila lamin in vivo: site identification during both M-phase (meiosis) and interphase by electrospray ionization tandem mass spectrometry
    U Schneider
    Department of Biochemistry, Biozentrum of the University of Basel, Switzerland
    Biochemistry 38:4620-32. 1999
    ..tandem mass spectrometry, we determined the phosphorylation sites of both interphase and M-phase isoforms of nuclear lamin Dm from Drosophila melanogaster...
  3. pmc Molecular genetic analysis of the nested Drosophila melanogaster lamin C gene
    Sandra R Schulze
    Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Genetics 171:185-96. 2005
    ..These transgenic animals display a nuclear lamin aggregation phenotype remarkably similar to that observed when human mutant A-type lamins are expressed in ..
  4. ncbi Caspases function in autophagic programmed cell death in Drosophila
    Damali N Martin
    Center for Biosystems Research, University of Maryland Biotechnology Institute, and Department of Cell Biology, University of Maryland, College Park, Maryland 20742, USA
    Development 131:275-84. 2004
    ..salivary gland destruction, and coincide with increased levels of active Caspase 3 and a cleaved form of nuclear Lamin. Mutations in the steroid-regulated genes beta FTZ-F1, E93, BR-C and E74A that prevent salivary gland cell ..
  5. pmc Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila
    Deborah L Berry
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    Cell 131:1137-48. 2007
    ....
  6. pmc Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations
    J C Fung
    Graduate Group in Biophysics, University of California, San Francisco, California 94143 0554, USA
    J Cell Biol 141:5-20. 1998
    ..Thus, we conclude that simple random contacts through diffusion could suffice to allow pairing of homologous sites...
  7. ncbi Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos
    M R Paddy
    Center for Structural Biology and Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610 0235, USA
    J Cell Sci 109:591-607. 1996
    ..The process of lamin delocalization and dispersal, typical of 'open' forms of mitosis, does not begin until about the time the final, ..
  8. ncbi Stage-specific chromosomal association of Drosophila dMBD2/3 during genome activation
    Joachim Marhold
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Chromosoma 111:13-21. 2002
    ..Our results indicate that dMBD2/3 forms specialized nuclear compartments to keep certain genes epigenetically silenced during genome activation...
  9. pmc drICE is an essential caspase required for apoptotic activity in Drosophila cells
    A G Fraser
    Biochemistry of the Cell Nucleus Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 16:6192-9. 1997
    ..expression or cycloheximide treatment contain a caspase activity with DEVD specificity which can cleave p35, lamin DmO, drICE and DCP-1 in vitro, and which can trigger chromatin condensation in isolated nuclei...
  10. pmc Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract
    N Ulitzur
    Department of Genetics, Hebrew University of Jerusalem, Israel
    J Cell Biol 119:17-25. 1992
    The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a Drosophila in vitro assembly system that reconstitutes nuclei from added sperm chromatin or naked DNA...
  11. ncbi Immunoaffinity purification and functional characterization of interphase and meiotic Drosophila nuclear lamin isoforms
    L Lin
    Department of Pharmacological Sciences, State University of New York, Stony Brook, 11794 8651
    J Biol Chem 265:12596-601. 1990
    Three isoforms of a single nuclear lamin have been identified in Drosophila. Two, lamins Dm1 and Dm2, are present during interphase and are apparently in equilibrium with each other in vivo...
  12. pmc Biosynthesis and interconversion of Drosophila nuclear lamin isoforms during normal growth and in response to heat shock
    D E Smith
    J Cell Biol 105:771-90. 1987
    ..Both lamins are apparently derived posttranslationally from a single, primary translation product, lamin Dm0. A pathway has been established whereby lamin Dm0 is processed almost immediately upon synthesis in the ..
  13. ncbi Monoclonal antibodies prepared against the major Drosophila nuclear Matrix-pore complex-lamina glycoprotein bind specifically to the nuclear envelope in situ
    A J Filson
    J Biol Chem 260:3164-72. 1985
    ..Ottaviano, Y., and Kondor-Koch, C. (1982) J. Cell Biol. 95, 826-837)...
  14. ncbi Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins
    D Riemer
    Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Goettingen, FRG
    J Cell Sci 108:3189-98. 1995
    ..the pG-IF encoded protein is expressed and whether it encodes a cytoplasmic intermediate filament protein or a nuclear lamin we raised antibodies against the recombinant pG-IF protein...
  15. ncbi A cDNA from Drosophila melanogaster encodes a lamin C-like intermediate filament protein
    C A Bossie
    Department of Pharmacology, UMDNJ Robert Wood Johnson Medical School, Piscataway 08854
    J Cell Sci 104:1263-72. 1993
    ..Of these, one has been identified as the previously characterized Drosophila nuclear lamin cDNA, Dm0, and a second, pG-IF, demonstrates homology to Dm0 by cross hybridization on Southern blots...
  16. ncbi Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody
    N Stuurman
    Department of Pharmacological Sciences, University Medical Center, SUNY at Stony Brook 11794 8651, USA
    J Cell Sci 108:3137-44. 1995
    The Drosophila nuclear lamin is highly phosphorylated during interphase. Two interphase isoforms, differing in degree of phosphorylation, can be distinguished by one-dimensional SDS-polyacrylamide gel electrophoresis...
  17. ncbi DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ
    E A Baricheva
    Department of Pharmacological Sciences, University Medical Center, State University of New York at Stony Brook 11794 8651, USA
    Gene 171:171-6. 1996
    A DNA fragment designated lambda 20p1.4 binds in vitro to polymerized Drosophila melanogaster lamin. In situ hybridization of lambda 20p1...
  18. pmc Specific interactions of chromatin with the nuclear envelope: positional determination within the nucleus in Drosophila melanogaster
    W F Marshall
    Department Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    Mol Biol Cell 7:825-42. 1996
    ....
  19. ncbi Identification of a conserved phosphorylation site modulating nuclear lamin polymerization
    N Stuurman
    Department of Pharmacological Sciences, SUNY at Stony Brook, NY 11794 8651, USA
    FEBS Lett 401:171-4. 1997
    ..A co-immunoprecipitation assay, employing Drosophila nuclear lamin Dm0 fragments was used to study the effect of phosphorylation on head-to-tail binding...
  20. pmc Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae
    B Lenz-Böhme
    Max Planck Institut für Hirnforschung, Abteilung Neurochemie, D 60528 Frankfurt am Main, Germany
    J Cell Biol 137:1001-16. 1997
    ..Here, we describe a Drosophila lamin Dm0 mutant resulting from a P element insertion into the first intron of the Dm0 gene...
  21. pmc Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development
    R Ashery-Padan
    Department of Genetics, The Life Sciences Institute, The Hebrew University of Jerusalem, Israel
    Mol Cell Biol 17:4114-23. 1997
    ..With the exception of sperm cells, otefin colocalizes with lamin Dm0 derivatives in situ and presumably in vivo and is present in all somatic cells examined during the different ..
  22. ncbi Assembly of A- and B-type lamins studied in vivo with the baculovirus system
    M Klapper
    Division of Electron Microscopy, Biocenter of the University of Wurzburg, Germany
    J Cell Sci 110:2519-32. 1997
    ..Xenopus lamin A), a probable A-type lamin (Drosophila lamin C), two B-type lamins (Xenopus lamin LI, Drosophila lamin Dmo), and two mutants of Xenopus lamin A in Sf9 cells...
  23. ncbi In vivo association of lamins with nucleic acids in Drosophila melanogaster
    R Rzepecki
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, State University of New York at Stony Brook, Stony Brook, NY 11794 8651, USA
    J Cell Sci 111:121-9. 1998
    ..Interactions with DNA as well as RNA were detected. In contrast, interaction of nucleic acids with mitotic lamin was not observed...
  24. ncbi Homologous association of the Bithorax-Complex during embryogenesis: consequences for transvection in Drosophila melanogaster
    M J Gemkow
    Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, FRG
    Development 125:4541-52. 1998
    ..We examined the onset of pairing of the chromosome 4 as well as of loci near the centromere of chromosome 3 and near the telomere of 3R in order to test models for the mechanism of homologue pairing...
  25. pmc The tail domain of lamin Dm0 binds histones H2A and H2B
    M Goldberg
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 96:2852-7. 1999
    ..Deletion mutants of Drosophila lamin Dm0 were expressed to map regions of the protein that are required for its binding to chromosomes...
  26. pmc A putative exchange factor for Rho1 GTPase is required for initiation of cytokinesis in Drosophila
    S N Prokopenko
    Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030 USA
    Genes Dev 13:2301-14. 1999
    ..Our results identify Pbl as a RhoGEF specifically required for cytokinesis and linked through Rho1 activity to the reorganization of the actin cytoskeleton at the cleavage furrow...
  27. ncbi The balance between two isoforms of the Drosophila RNA-binding protein how controls tendon cell differentiation
    H Nabel-Rosen
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
    Mol Cell 4:573-84. 1999
    ..This inhibition is likely to be counteracted by the short How(S) protein, present in both nucleus and cytoplasm, which is upregulated in the muscle-bound tendon cell following EGF receptor activation...
  28. pmc members only encodes a Drosophila nucleoporin required for rel protein import and immune response activation
    A E Uv
    Umeâ Center for Molecular Pathogenesis UCMP, Umea University, S 90187 Umea, Sweden
    Genes Dev 14:1945-57. 2000
    ..Our results demonstrate that distinct nuclear import events require different nucleoporins in vivo and suggest a regulatory role for mbo in signal transduction...
  29. pmc The Ketel gene encodes a Drosophila homologue of importin-beta
    M Lippai
    Faculty of General Medicine, Department of Biology, University of Szeged, H 6720 Szeged, Hungary
    Genetics 156:1889-900. 2000
    ..The Ketel gene appears to be ubiquitously expressed in embryonic cells. Western blot analysis revealed that the Ketel gene is not expressed in several larval cell types of late third instar larvae...
  30. pmc Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix
    D L Walker
    Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA
    J Cell Biol 151:1401-12. 2000
    ..Thus, the identification and characterization of skeletor represents the first direct molecular evidence for the existence of a complete spindle matrix that forms within the nucleus before microtubule spindle formation...
  31. ncbi The fly caspases
    S Kumar
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    Cell Death Differ 7:1039-44. 2000
    ..The presence of multiple caspases in Drosophila indicates that apoptotic pathways in insects are likely to be as complex as in vertebrates...
  32. ncbi A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila
    K Guillemin
    Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305 5307, USA
    Nat Cell Biol 3:848-51. 2001
    ..Here we present evidence that a nuclear lamin is also required for cytoplasmic organization in two highly polarized cell types...
  33. ncbi Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation of Gurken through Chk2/Mnk
    Uri Abdu
    HHMI, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Curr Biol 12:1645-51. 2002
    ..However, the components of this checkpoint in flies have not been completely elucidated...
  34. pmc Visualization of chromatin domains created by the gypsy insulator of Drosophila
    Keith Byrd
    Department of Biology, Johns Hopkins University, 3400 N Charles Street, Baltimore, MD 21218, USA
    J Cell Biol 162:565-74. 2003
    ..These results suggest that the gypsy insulator might establish higher-order domains of chromatin structure and regulate nuclear organization by tethering the DNA to the nuclear matrix and creating chromatin loops...
  35. pmc The functions of Klarsicht and nuclear lamin in developmentally regulated nuclear migrations of photoreceptor cells in the Drosophila eye
    Kristin Patterson
    Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA
    Mol Biol Cell 15:600-10. 2004
    ..In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm(0), which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear ..
  36. ncbi The lamin B receptor of Drosophila melanogaster
    Nicole Wagner
    Division of Electron Microscopy, Biocenter of the University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Cell Sci 117:2015-28. 2004
    ..Third, dLBR can be shown by co-immunoprecipitations and in vitro binding assays to bind to the Drosophila B-type lamin Dm0. Fourth, the N-terminal domain of dLBR is sufficient for in vitro binding to sperm chromatin and lamin Dm0...
  37. ncbi The lamin CxxM motif promotes nuclear membrane growth
    Kristina Prüfert
    Division of Electron Microscopy, Biocenter of the University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    J Cell Sci 117:6105-16. 2004
    ..the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins...
  38. ncbi The ubiquitin ligase dTopors directs the nuclear organization of a chromatin insulator
    Maya Capelson
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Mol Cell 20:105-16. 2005
    ..dTopors associates with the nuclear lamina, and mutations in lamin disrupt dTopors localization as well as nuclear organization and activity of the gypsy insulator...
  39. ncbi Characterization of the Drosophila melanogaster genome at the nuclear lamina
    Helen Pickersgill
    Department of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    Nat Genet 38:1005-14. 2006
    ..Using an in vivo approach, we identified approximately 500 Drosophila melanogaster genes that interact with B-type lamin (Lam)...
  40. ncbi Lamin B1 duplications cause autosomal dominant leukodystrophy
    Quasar S Padiath
    Department of Neurology, University of California, San Francisco, San Francisco, California 94158, USA
    Nat Genet 38:1114-23. 2006
    ..This is the first human disease attributable to mutations in the gene encoding lamin B1. Antibodies to lamin B are found in individuals with autoimmune diseases, and it is also an antigen recognized by a monoclonal antibody ..
  41. ncbi The nucleoporin Nup214 sequesters CRM1 at the nuclear rim and modulates NFkappaB activation in Drosophila
    Nikos Xylourgidis
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 10691, Stockholm, Sweden
    J Cell Sci 119:4409-19. 2006
    ..We hypothesize that variation in levels of the Nup214-Nup88 complex at the pore changes the amount of NPC-bound CRM1 and influences the relative strength and duration of NFkappaB signaling responses...
  42. ncbi Specific and conserved sequences in D. melanogaster and C. elegans lamins and histone H2A mediate the attachment of lamins to chromosomes
    Anna Mattout
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904 Israel
    J Cell Sci 120:77-85. 2007
    ..have mapped a region between the rod domain and the Ig fold in the tail domain of Drosophila melanogaster lamin Dm0, which binds chromatin in vitro via the histone H2A/H2B dimer...
  43. ncbi Invertebrate lamins
    Shai Melcer
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Exp Cell Res 313:2157-66. 2007
    ..Studying lamins in invertebrate species has unique advantages including the smaller number of lamin genes in the invertebrate genomes and powerful genetic analyses in Caenorhabditis elegans and Drosophila ..
  44. pmc Characterization of lamin mutation phenotypes in Drosophila and comparison to human laminopathies
    Andrés Muñoz-Alarcón
    Department of Life Sciences, Södertörns högskola, Huddinge, Sweden Department of Biosciences and Nutrition, Karolinska Institute, Stockholm, Sweden
    PLoS ONE 2:e532. 2007
    ..It is therefore of interest to examine the effects of mutations in lamin genes. Mutations in the mammalian lamin A/C gene cause a range of diseases, collectively called laminopathies, that include muscular dystrophies and ..
  45. ncbi SUUR joins separate subsets of PcG, HP1 and B-type lamin targets in Drosophila
    Alexey V Pindyurin
    Institute of Cytology and Genetics of Siberian Division, Russian Academy of Sciences, Novosibirsk 630090, Russia
    J Cell Sci 120:2344-51. 2007
    ..PcG proteins (Pc and Esc; Polycomb and Extra sexcombs), heterochromatic proteins [HP1 and SU(VAR)3-9] and B-type lamin. The SUUR binding profile negatively correlates with the DNA polytenization levels of salivary gland polytene ..
  46. doi The farnesylated nuclear proteins KUGELKERN and LAMIN B promote aging-like phenotypes in Drosophila flies
    Annely Brandt
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, DKFZ ZMBH Allianz, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Aging Cell 7:541-51. 2008
    ..The expression of laminDelta50, a constitutively active lamin A splicing variant in Hutchinson-Gilford progeria syndrome patients, leads to the lobulation of the nuclear ..
  47. pmc Tissue-specific defects are caused by loss of the Drosophila MAN1 LEM domain protein
    Belinda S Pinto
    Molecular and Cellular Biology Program, University of Iowa, College of Medicine, Iowa City, Iowa 52242, USA
    Genetics 180:133-45. 2008
    ..The tissue-specific, age-enhanced dMAN1 mutant phenotypes are reminiscent of human laminopathies, suggesting that studies in Drosophila will provide insights into lamina dysfunction associated with disease...
  48. doi Towards a Drosophila model of Hutchinson-Gilford progeria syndrome
    Gemma S Beard
    Division of Biosciences, Centre for Cell and Chromosome Biology, School of Health Sciences and Social Care, Brunel University, Uxbridge, Middlesex, UK
    Biochem Soc Trans 36:1389-92. 2008
    The laminopathy Hutchinson-Gilford progeria syndrome (HGPS) is caused by the mutant lamin A protein progerin and leads to premature aging of affected children...
  49. pmc Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
    Mariana Lince-Faria
    Instituto de Biologia Molecular e Celular, Faculdade de Medicina, Universidade do Porto, 4150 180 Porto, Portugal
    J Cell Biol 184:647-57. 2009
    ....
  50. pmc A comparative study of Drosophila and human A-type lamins
    Sandra R Schulze
    Department of Biology, Western Washington University, Bellingham, Washington, United States of America
    PLoS ONE 4:e7564. 2009
    ..The Drosophila genome possesses two genes encoding lamins, Lamin C and lamin Dm(0), which have been designated A- and B-type, respectively, based on their expression profile and structural ..
  51. pmc Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope
    Gul Civelekoglu-Scholey
    Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    J Cell Biol 188:49-68. 2010
    ..Functional perturbation and modeling suggest that this range can be extended significantly by a disassembling lamin-B envelope that surrounds the prometaphase spindle and augments the finely tuned, antagonistic kinesin force ..
  52. doi Sunspot, a link between Wingless signaling and endoreplication in Drosophila
    Kenzui Taniue
    Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Development 137:1755-64. 2010
    ..In addition, Ssp was found not to be involved in the signaling pathway mediated by Arm associated with dTCF. Our findings indicate that Arm controls development in part by regulating the function of Ssp...
  53. pmc The role of Drosophila Lamin C in muscle function and gene expression
    George Dialynas
    Department of Biochemistry, University of Iowa, Iowa City, IA 52241, USA
    Development 137:3067-77. 2010
    ..The GAL4-UAS system was used to express wild-type and mutant forms of Lamin C (the presumed Drosophila A-type lamin), in an otherwise wild-type background...
  54. pmc The meiotic recombination checkpoint suppresses NHK-1 kinase to prevent reorganisation of the oocyte nucleus in Drosophila
    Oscar M Lancaster
    The Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Genet 6:e1001179. 2010
    ..Therefore we propose that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired...
  55. pmc The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome
    Joke G van Bemmel
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 5:e15013. 2010
    ..Our results provide insights into the evolution of LAD organization and identify SU(HW) as a fine-tuner of genome - NL interactions...
  56. doi Flybow: genetic multicolor cell labeling for neural circuit analysis in Drosophila melanogaster
    Dafni Hadjieconomou
    Medical Research Council National Institute for Medical Research, Division of Molecular Neurobiology, London, UK
    Nat Methods 8:260-6. 2011
    ....
  57. pmc History and phylogeny of intermediate filaments: now in insects
    Harald Herrmann
    Group Functional Architecture of the Cell B065 German Cancer Research Center DKFZ Im Neuenheimer Feld 280 D 69120 Heidelberg, Germany
    BMC Biol 9:16. 2011
    ..Here we briefly review the history of research on intermediate filaments, and discuss the implications of this latest finding in the context of what is known of their structure and functions...
  58. doi Identification of new in vivo phosphosites on lamin Dm - the evidence of heterogeneity of phosphorylation sites in different Drosophila tissues
    Magdalena Zaremba-Czogalla
    Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wrocław, Przybyszewskiego St 63 78, 51 148 Wrocław, Poland
    Nucleus 2:478-88. 2011
    ..Here, we report the identification of new in vivo phosphorylation sites on Drosophila melanogater lamin Dm using immunoisolation and mass spectrometry with collision-induced peptide fragmentation (Electrospray-Linear ..
  59. ncbi Molecular analysis of the Drosophila nuclear lamin gene
    M Osman
    Department of Genetics, Hebrew University, Jerusalem, Israel
    Genomics 8:217-24. 1990
    A complete nucleotide sequence of a 4.2-kb genomic fragment containing the Drosophila lamin gene and flanking sequences is presented...
  60. ncbi Interphase nuclear envelope lamins form a discontinuous network that interacts with only a fraction of the chromatin in the nuclear periphery
    M R Paddy
    Structural Biology Unit, Howard Hughes Medical Institute, San Francisco, California
    Cell 62:89-106. 1990
    Antibodies directed against nuclear envelope lamin proteins have been used in conjunction with three-dimensional light and electron microscope methodologies to determine the spatial organization of lamins in diploid interphase nuclei and ..
  61. ncbi Binding of matrix attachment regions to nuclear lamin is mediated by the rod domain and depends on the lamin polymerization state
    K Zhao
    Department of Genetics, Life Sciences Institute, Hebrew University of Jerusalem, Israel
    FEBS Lett 380:161-4. 1996
    ..M/SARs bind in vitro to lamin polymers. We show that the polymerized alpha-helical rod domain of lamin Dm0 provides by itself the specific binding to the ftz M/SAR...
  62. ncbi In vitro assembly of Drosophila lamin Dm0--lamin polymerization properties are conserved
    B Sasse
    M E Müller Institute for Microscopy, Biozentrum, University of Basel, Switzerland
    Eur J Biochem 250:30-8. 1997
    ..conditions where vertebrate cytoplasmic IF proteins form tetramers consisting of laterally associated dimers, nuclear lamin dimers associate longitudinally into head-to-tail polymers...
  63. ncbi Ectopic overexpression of Drosophila lamin C is stage-specific lethal
    N Stuurman
    Maurice E Müller Institut am Biozentrum, Universitat Basel, Klingelbergstrasse 70, Basel, CH 4055, Switzerland
    Exp Cell Res 248:350-7. 1999
    ..we transformed Drosophila melanogaster with a construct containing the hsp70 promoter followed by the Drosophila lamin C (an analog of vertebrate A-type lamins) cDNA...
  64. ncbi During both interphase and mitosis, DNA topoisomerase II interacts with DNA as well as RNA through the protein's C-terminal domain
    R Rzepecki
    The Department of Pharmacological Sciences, University Medical Center, The State University of New York at Stony Brook, Stony Brook, NY 11794 8651, USA
    J Cell Sci 113:1635-47. 2000
    ..Our observations suggest that cell cycle-dependent changes in the distribution, nucleic acid interactions and enzymatic activity of topo II are regulated, at least in part, by phosphorylation/dephosphorylation...
  65. ncbi Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila
    Eli Arama
    Howard Hughes Medical Institute, Strang Laboratory of Cancer Research, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Dev Cell 4:687-97. 2003
    ..These observations suggest that an apoptosis-like mechanism is required for spermatid differentiation in Drosophila...
  66. ncbi Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila
    Kazuhiro Furukawa
    Department of Chemistry, Faculty of Science, Niigata University, Niigata 950 2181, Japan
    J Cell Sci 116:3811-23. 2003
    ..At the light microscopic level, grossly aberrant nuclear lamina structure and B-type lamin distribution correlated well with the loss of detectable amounts of BAF protein from nuclei...
  67. ncbi Two novel LEM-domain proteins are splice products of the annotated Drosophila melanogaster gene CG9424 (Bocksbeutel)
    Nicole Wagner
    Division of Electron Microscopy, Biocenter of the University of Wurzburg, Wurzburg, Germany
    Eur J Cell Biol 82:605-16. 2004
    ..By RNA interference we have down-regulated the expression of Bocksbeutel, BAF, otefin, and lamin DmO in Drosophila Kc167 cells...
  68. ncbi Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis
    Tasman J Daish
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia
    Dev Cell 7:909-15. 2004
    ..Our results indicate that DRONC is involved in specific developmental cell death pathways and that in some tissues, effector caspase activation and cell death can occur independently of DRONC...
  69. ncbi Null mutants of Drosophila B-type lamin Dm(0) show aberrant tissue differentiation rather than obvious nuclear shape distortion or specific defects during cell proliferation
    Shinichi Osouda
    Department of Chemistry, Faculty of Science, Niigata University, Niigata 950 2181, Japan
    Dev Biol 284:219-32. 2005
    ..Although in all these mutants, lamin Dm(0) protein was undetectable in neuroblasts and imaginal disc cells from the second instar larval stage onward, ..
  70. ncbi Developmental control of nuclear size and shape by Kugelkern and Kurzkern
    Annely Brandt
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Curr Biol 16:543-52. 2006
    ..We investigated this question in early Drosophila embryos, where the nuclear shape changes from spherical to ellipsoidal together with a 2.5-fold increase in nuclear length during cellularization...
  71. pmc NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus
    Oscar M Lancaster
    The Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 179:817-24. 2007
    ..We propose that BAF phosphorylation by NHK-1 disrupts anchorage of chromosomes to the nuclear envelope, allowing karyosome formation in oocytes. These data provide the first mechanistic insight into how the karyosome forms...
  72. pmc Interconversion of Drosophila nuclear lamin isoforms during oogenesis, early embryogenesis, and upon entry of cultured cells into mitosis
    D E Smith
    Department of Pharmacological Sciences, State University of New York, Stony Brook 11794 8651
    J Cell Biol 108:255-65. 1989
    Two isoforms of a single nuclear lamin, distinguishable on one-dimensional SDS-polyacrylamide gels, have previously been identified in Drosophila nuclei during interphase...
  73. pmc Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene
    Y Gruenbaum
    Department of Biochemistry and Biophysics, University of California at San Francisco 94143
    J Cell Biol 106:585-96. 1988
    ..Southern blot analyses of genomic DNA are consistent with the notion that a single or only a few highly similar genes encoding Drosophila nuclear lamin Dm0 exist in the genome.
  74. ncbi The RRM protein NonA from Drosophila forms a complex with the RRM proteins Hrb87F and S5 and the Zn finger protein PEP on hnRNA
    I Reim
    Institut für Biologie Abt Zytogenetik, Humboldt Universitat zu Berlin, Berlin, D 10115, Germany
    Exp Cell Res 253:573-86. 1999
    ..Like NonA, X4/PEP, S5, and P11/Hrb87F are present on active sites on polytene chromosomes. The precipitated NonA complex is enriched for certain protein encoding RNAs, notably, histone H3 and H4 RNA...
  75. ncbi Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 25:307-16. 2000
    ..Thus, RhoA is an important regulator of dendritic morphogenesis, while distinct mechanisms are used for axonal morphogenesis...
  76. ncbi Expression of the vertebrate Gli proteins in Drosophila reveals a distribution of activator and repressor activities
    P Aza-Blanc
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Development 127:4293-301. 2000
    ..These observations suggest that the separate activator and repressor functions of Ci are unevenly partitioned among the three Glis, yielding proteins with related yet distinct properties...
  77. ncbi Paternal chromosome incorporation into the zygote nucleus is controlled by maternal haploid in Drosophila
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre 1918, Villeurbanne Cedex, 69622, France
    Dev Biol 231:383-96. 2001
    ..The mh phenotype is highly reminiscent of the early developmental defects observed in eggs fertilized by ms(3)K81 mutant males and in eggs produced in incompatible crosses of Drosophila harboring the endosymbiont bacteria Wolbachia...
  78. ncbi The JIL-1 tandem kinase mediates histone H3 phosphorylation and is required for maintenance of chromatin structure in Drosophila
    Y Wang
    Department of Zoology and Genetics, 3154 Molecular Biology Building, Iowa State University, Ames, IA 50011, USA
    Cell 105:433-43. 2001
    ..We propose a model where JIL-1 kinase activity is required for maintaining the structure of the more open chromatin regions that facilitate gene transcription...
  79. ncbi Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis
    Tzu Kang Sang
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA
    Development 129:143-54. 2002
    ..Loss of Eyc function results in a lethal failure of nuclear envelope assembly in early zygotic divisions. Phenotypes resulting from eyc mutations provide the first in vivo evidence for a role for p47 in membrane biogenesis...
  80. ncbi Dcas is required for importin-alpha3 nuclear export and mechano-sensory organ cell fate specification in Drosophila
    Hildegard Tekotte
    Wellcome Trust Centre for Cell Biology, ICMB, King s Buildings, University of Edinburgh, Edinburgh, Scotland, EH9 3JR, UK
    Dev Biol 244:396-406. 2002
    ..We propose that mutations in house keeping genes often cause specific developmental phenotypes, such as those observed in many human genetic disorders...
  81. pmc Nuclear location of a chromatin insulator in Drosophila melanogaster
    Qinghao Xu
    Department of Cellular Biology, The University of Georgia, Athens, GA 30602, USA
    J Cell Sci 117:1025-32. 2004
    ..Our results indicate that the suHw insulator can function in the nuclear interior, possibly through local interactions with chromatin components or other nuclear structures...
  82. ncbi The Drosophila RCC1 homolog, Bj1, regulates nucleocytoplasmic transport and neural differentiation during Drosophila development
    Wei Yang Shi
    Program in Developmental Biology, Washington University School of Medicine, St Louis, MO 63110, USA
    Dev Biol 270:106-21. 2004
    ..Finally, we show that dRCC1 is required for cell proliferation and/or survival during germline, eye and wing development and that dRCC1 appears to facilitate apoptosis...
  83. ncbi Pygopus and Legless target Armadillo/beta-catenin to the nucleus to enable its transcriptional co-activator function
    Fiona M Townsley
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Nat Cell Biol 6:626-33. 2004
    ..This indicates that Pygo and Lgs function in targeting Armadillo/beta-catenin to the nucleus, thus ensuring its availability to TCF during Wnt signalling...
  84. pmc Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda
    Carolina Bartolomé
    Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, UK
    Genetics 169:1495-507. 2005
    ..The joint intensity of selection and biased gene conversion, in terms of the product of effective population size and the sum of the selection and conversion coefficients, was estimated to be approximately 0.65...
  85. pmc Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration
    Brian McHugh
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 167:673-86. 2004
    ..present in wild-type larval brains, is missing from homozygous tissue, and we show that IX-14/invadolysin cleaves lamin in vitro...
  86. ncbi Centrosome inheritance in the male germ line of Drosophila requires hu-li tai-shao function
    Patricia G Wilson
    Department of Biology, Georgia State University, MSC 8L0389, 33, Gilmer Street SE, Unit 8, Atlanta, GA 30303 3088, USA
    Cell Biol Int 29:360-9. 2005
    ..Collectively, these results indicate that hts function is necessary for centrosome inheritance in spermatocytes as well as for male fertility...
  87. ncbi The lamin Dm0 allele Ari3 acts as an enhancer of position effect variegation of the wm4 allele in Drosophila
    Xiaomin Bao
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 3154 Molecular Biology Building, Ames, Iowa 50011, USA
    Genetica 129:339-42. 2007
    ..To test this hypothesis we have examined the effect of a recently generated partial loss-of-function lamin Dm0 allele Ari3 on PEV of the wm4 allele in the Drosophila eye...
  88. pmc The Drosophila mitotic inhibitor Frühstart specifically binds to the hydrophobic patch of cyclins
    Pawel Gawlinski
    ZMBH, Im Neuenheimer Feld 282, Heidelberg 69120, Germany
    EMBO Rep 8:490-6. 2007
    ..We propose that binding of Frs to cyclins blocks the hydrophobic patch to interfere with Cdk1 substrate recognition...
  89. pmc Boundary element-associated factor 32B connects chromatin domains to the nuclear matrix
    Rashmi U Pathak
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
    Mol Cell Biol 27:4796-806. 2007
    ..These results suggest that boundary elements may function by tethering chromatin to nuclear architectural components and thereby provide a structural basis for compartmentalization of the genome into functionally independent domains...
  90. pmc Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
    Nafiseh Sabri
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 10691 Stockholm, Sweden
    J Cell Biol 178:557-65. 2007
    ..Our data suggest that peripheral metazoan FG nucleoporins have distinct functions in nuclear protein transport events...
  91. pmc Cotranscriptional recruitment of the dosage compensation complex to X-linked target genes
    Jop Kind
    European Molecular Biology Laboratory, Heidelberg 69117, Germany
    Genes Dev 21:2030-40. 2007
    ..We propose a model of dynamic MSL complex binding to active genes based on exposed DNA target elements...
  92. doi UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization in Drosophila
    Carine Meignin
    Department of Biochemistry, The University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Dev Biol 315:89-98. 2008
    ..The first is in the nucleus for mRNA export of most transcripts from the nucleus. The second is in the cytoplasm for remodeling the transacting factors that decorate mRNA and dictate its cytoplasmic destination...
  93. pmc Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis
    Floris Bosveld
    Department of Cell Biology, Section of Radiation and Stress Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    BMC Res Notes 1:75. 2008
    ..Mutations in Drosophila dPPCS disrupt female fecundity and in this study we analyzed the female sterile phenotype of dPPCS mutants in detail...
  94. pmc Bicaudal is a conserved substrate for Drosophila and mammalian caspases and is essential for cell survival
    Emma M Creagh
    Molecular Cell Biology Laboratory, Department of Genetics, Smurfit Institute, Trinity College, Dublin, Ireland corrected
    PLoS ONE 4:e5055. 2009
    ..These data suggest that bicaudal/betaNAC is essential for cell survival and is a conserved target of caspases from flies to man...
  95. doi Nucleoporins directly stimulate expression of developmental and cell-cycle genes inside the nucleoplasm
    Bernike Kalverda
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 140:360-71. 2010
    ..Thus, nucleoporins stimulate developmental and cell-cycle gene expression away from the NPC by interacting with these genes inside the nucleoplasm...
  96. pmc Inactivation of both Foxo and reaper promotes long-term adult neurogenesis in Drosophila
    Sarah E Siegrist
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720 3200, USA
    Curr Biol 20:643-8. 2010
    ..Thus, inhibition of the pathways that normally function to eliminate neural stem cells during development enables adult neurogenesis...
  97. pmc Developmental regulation of Drosophila DNA topoisomerase II
    A M Whalen
    Department of Pharmacological Sciences, State University of New York, Stony Brook 11794 8651
    J Cell Biol 112:203-13. 1991
    ..fluctuations were recorded at different stages, these fluctuations were paralleled by changes in levels of the nuclear lamin, a nuclear structural protein used as an internal standard...
  98. ncbi Independent roles of centrosomes and DNA in organizing the Drosophila cytoskeleton
    G K Yasuda
    Department of Genetics, University of Washington, Seattle 98195
    Development 111:379-91. 1991
    ..We speculate that the principles of cytoskeleton organization in this system may be different from those of the Xenopus "open" mitotic system...
  99. ncbi Persistence of major nuclear envelope antigens in an envelope-like structure during mitosis in Drosophila melanogaster embryos
    A Harel
    Department of Genetics, Hebrew University of Jerusalem, Israel
    J Cell Sci 94:463-70. 1989
    ..Two of these proteins, lamin and otefin, a newly characterized nuclear envelope polypeptide with an apparent Mr of 53,000, are apparently ..
  100. ncbi Disassembly of the Drosophila nuclear lamina in a homologous cell-free system
    N Maus
    Graduate Program in Genetics, State University of New York at Stony Brook 11794 8651, USA
    J Cell Sci 108:2027-35. 1995
    ..cell-free extract of these oocytes catalyzes apparent disassembly of purified Drosophila nuclei as well as of nuclear lamin polymers formed in vitro from isolated interphase lamins...
  101. ncbi [Beta-heterochromatin in Drosophila: molecular organization and function. Molecular biological analysis of a MAR/SAR DNA sequence in centromere heterochromatin of Drosophila melanogaster]
    S S Bogachev
    Department of Pharmacological Sciences, University Medical Center at Stony Brook, State University of New York at Stony Brook 11794 8651, USA
    Genetika 32:240-51. 1996
    ..The region of the delta 20p1.4 fragment between nucleotides 350 and 905 polymerizes with purified lamin from D. melanogaster...

Research Grants2

  1. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
    ..Thus, we exploit the powerful genetics of Drosophila to elucidate efficiently the molecules and gene pathways needed to start development in humans. ..
  2. Molecular Genetics of Polyglutamine-Induced Degeneration
    George Jackson; Fiscal Year: 2003
    ..abstract not available ..