Hsp70Aa

Summary

Gene Symbol: Hsp70Aa
Description: Heat-shock-protein-70Aa
Alias: 87A, CG31366, Dm-hsp70, DmHSP70AA, Dmel\CG31366, HSP70, Hsp 70, Hsp-70, Hsp-70Aa, Hsp70, Hsp70A, Hsp70a, dHsp70, dhsp70, dhsp70Aa, hs70, hsp 70, hsp-70, hsp70, hsp70 87A, hsp70 Aa, hsp70(87A), hsp70A, hsp70Aa, hsp70a, CG31366 gene product from transcript CG31366-RA, 87A locus, CG31366-PA, Hsp70Aa-PA, heat shock 70, heat shock protein-70, heat-shock protein 70, hsp70 87A, protein 70
Species: fruit fly

Top Publications

  1. pmc Chromatin structure, not DNA sequence specificity, is the primary determinant of topoisomerase II sites of action in vivo
    A Udvardy
    Department of Biology, Princeton University, New Jersey 08544
    Mol Cell Biol 11:4973-84. 1991
  2. pmc Distinct mechanisms underlying tolerance to intermittent and constant hypoxia in Drosophila melanogaster
    Priti Azad
    Department of Pediatrics, Section of Respiratory Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 4:e5371. 2009
  3. ncbi Polymerase processivity and termination on Drosophila heat shock genes
    C Giardina
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    J Biol Chem 268:23806-11. 1993
  4. ncbi Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo
    Abbie Saunders
    Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA
    Science 301:1094-6. 2003
  5. ncbi Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex
    Sheryl T Smith
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Nat Cell Biol 6:162-7. 2004
  6. pmc Metal-responsive transcription factor (MTF-1) handles both extremes, copper load and copper starvation, by activating different genes
    Anand Selvaraj
    Institute of Molecular Biology, University of Zurich, CH 8057, Zurich, Switzerland
    Genes Dev 19:891-6. 2005
  7. pmc Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II
    So Hee Kwon
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 24:2133-45. 2010
  8. ncbi S-element insertions are associated with the evolution of the Hsp70 genes in Drosophila melanogaster
    Xulio Maside
    Institute of Cell, Animal and Population Biology, University of Edinburgh, EH9 3JT, Edinburgh, United Kingdom
    Curr Biol 12:1686-91. 2002
  9. pmc A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay
    Isabelle Behm-Ansmant
    Max Planck Institute for Developmental Biology, Tubingen, Germany
    EMBO J 26:1591-601. 2007
  10. ncbi Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster
    D Ish-Horowicz
    Cell 18:1351-8. 1979

Research Grants

Scientific Experts

Detail Information

Publications133 found, 100 shown here

  1. pmc Chromatin structure, not DNA sequence specificity, is the primary determinant of topoisomerase II sites of action in vivo
    A Udvardy
    Department of Biology, Princeton University, New Jersey 08544
    Mol Cell Biol 11:4973-84. 1991
    ..We suggest that chromatin organization may provide a general mechanism for generating specificity in a wide range of DNA-protein interactions in vivo...
  2. pmc Distinct mechanisms underlying tolerance to intermittent and constant hypoxia in Drosophila melanogaster
    Priti Azad
    Department of Pediatrics, Section of Respiratory Medicine, University of California San Diego, La Jolla, California, United States of America
    PLoS ONE 4:e5371. 2009
    ..Our current genome-wide study is designed to investigate gene expression changes and identify protective mechanism(s) in D. melanogaster after exposure to severe (1% O(2)) intermittent or constant hypoxia...
  3. ncbi Polymerase processivity and termination on Drosophila heat shock genes
    C Giardina
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    J Biol Chem 268:23806-11. 1993
    ..Transcription of the uninduced Drosophila hsp70 gene is likewise restricted to the 5'-end, where the polymerase resides in a paused state...
  4. ncbi Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo
    Abbie Saunders
    Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA
    Science 301:1094-6. 2003
    ..Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms...
  5. ncbi Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex
    Sheryl T Smith
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Nat Cell Biol 6:162-7. 2004
    Rapid induction of the Drosophila melanogaster heat shock gene hsp70 is achieved through the binding of heat shock factor (HSF) to heat shock elements (HSEs) located upstream of the transcription start site (reviewed in ref. 3)...
  6. pmc Metal-responsive transcription factor (MTF-1) handles both extremes, copper load and copper starvation, by activating different genes
    Anand Selvaraj
    Institute of Molecular Biology, University of Zurich, CH 8057, Zurich, Switzerland
    Genes Dev 19:891-6. 2005
    ..Thus, a single transcription factor, MTF-1, plays a direct role in both copper detoxification and acquisition by inducing the expression of metallothioneins and of a copper importer, respectively...
  7. pmc Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II
    So Hee Kwon
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 24:2133-45. 2010
    ..The absence of HP1c partially impairs the recruitment of FACT into heat-shock loci and causes a defect in heat-shock gene expression. Thus, HP1c functions to recruit the FACT complex to RNA polymerase II...
  8. ncbi S-element insertions are associated with the evolution of the Hsp70 genes in Drosophila melanogaster
    Xulio Maside
    Institute of Cell, Animal and Population Biology, University of Edinburgh, EH9 3JT, Edinburgh, United Kingdom
    Curr Biol 12:1686-91. 2002
    ..fragments of an S-element are inserted into the 5' regions of three members of a heat shock gene family, Hsp70 (Hsp70Aa and Hsp70Ab in polytene chromosome band 87A, and Hsp70Bb in 87C)...
  9. pmc A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay
    Isabelle Behm-Ansmant
    Max Planck Institute for Developmental Biology, Tubingen, Germany
    EMBO J 26:1591-601. 2007
    ..These findings reveal a conserved role for PABPC1 in mRNA surveillance...
  10. ncbi Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci of D. melanogaster
    D Ish-Horowicz
    Cell 18:1351-8. 1979
    ..melanogaster. One of these codes for the 70,000 dalton heat shock protein (hsp 70) and is found at both loci...
  11. ncbi Stable binding of Drosophila heat shock factor to head-to-head and tail-to-tail repeats of a conserved 5 bp recognition unit
    O Perisic
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Cell 59:797-806. 1989
    ..We propose that trimers of HSF bind to DNA and that the number of HSF subunits in direct contact with DNA is determined by the number of correctly positioned 5 bp recognition units...
  12. ncbi Germline transformation used to define key features of heat-shock response elements
    H Xiao
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Science 239:1139-42. 1988
    ..Here, the sequence requirements for heat-shock induction are tested by Drosophila germline transformation with an hsp70-lacZ gene fused to a pair of synthetic HSEs...
  13. pmc Key features of heat shock regulatory elements
    J Amin
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101
    Mol Cell Biol 8:3761-9. 1988
    ..Second, the nucleotides at the two positions immediately upstream from GAA segments played an important role in defining the competence of regulatory elements...
  14. ncbi A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene
    H R Pelham
    Cell 30:517-28. 1982
    Deletion mutants of the Drosophila hsp 70 heat-shock gene have been assayed in COS cells using a vector that contains an SV40 replication origin...
  15. pmc Heat shock and recovery are mediated by different translational mechanisms
    B J DiDomenico
    Proc Natl Acad Sci U S A 79:6181-5. 1982
    ..Their repression was asynchronous, with translation of hsp70 always the first and translation of hsp82 always the last to be repressed...
  16. pmc Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress
    J C Wheeler
    Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340, USA
    Proc Natl Acad Sci U S A 92:10408-12. 1995
    Induction of Drosophila hsp70 protein was detected during aging in flight muscle and leg muscle in the absence of heat shock, using an hsp70-specific monoclonal antibody, and in transgenic flies containing hsp70-beta-galactosidase fusion ..
  17. ncbi Short transcripts of the ternary complex provide insight into RNA polymerase II elongational pausing
    E B Rasmussen
    Section of Biochemistry Molecular and Cell Biology, Cornell University, Ithaca New York, 14853, USA
    J Mol Biol 252:522-35. 1995
    Expression of the hsp70 gene of Drosophila melanogaster is controlled at the level of transcript elongation...
  18. pmc In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes
    E B Rasmussen
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Proc Natl Acad Sci U S A 90:7923-7. 1993
    The regulation of many eukaryotic genes occurs at the level of transcriptional elongation. On the uninduced hsp70 gene of Drosophila melanogaster, for example, an RNA polymerase II complex has initiated transcription but has paused early ..
  19. pmc Binding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila
    M Fernandes
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA
    Nucleic Acids Res 23:4799-804. 1995
    ..This last result suggests that close proximity of HSEs for cooperative binding of HSF is a result of protein-protein interactions near the point of DNA contact...
  20. ncbi HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences
    L S Shopland
    Section of Biochemistry, Molecular and Cell Biology, 417 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Chromosoma 105:158-71. 1996
    ..Finally, the distribution of the heat shock protein HSP70 is examined for its role in regulating the attenuated response of HSF to heat shock.
  21. ncbi Sodium salicylate decreases intracellular ATP, induces both heat shock factor binding and chromosomal puffing, but does not induce hsp 70 gene transcription in Drosophila
    N A Winegarden
    Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario, Canada L5L 1C6
    J Biol Chem 271:26971-80. 1996
    ..the induction of HSF binding and heat shock puffs in polytene chromosomes, we found no evidence for increased hsp 70 gene transcription suggesting that chromosomal puffing and gene transcription may be separable events...
  22. ncbi Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation
    J Lis
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Cold Spring Harb Symp Quant Biol 63:347-56. 1998
  23. ncbi Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70
    J M Warrick
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Genet 23:425-8. 1999
    ..melanogaster model of polyglutamine disease, we show that directed expression of the molecular chaperone HSP70 suppresses polyglutamine-induced neurodegeneration in vivo...
  24. pmc High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation
    E D Andrulis
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 14:2635-49. 2000
    ..These findings provide support for the roles of Spt5 in promoter-associated pausing and of Spt5 and Spt6 in transcriptional elongation in vivo...
  25. ncbi Reproductive costs of heat shock protein in transgenic Drosophila melanogaster
    R Silbermann
    Department of Ecology and Evolutionary Biology, Brown University, Providence, Rhode Island 02912, USA
    Evolution 54:2038-45. 2000
    ..The molecular chaperone hsp70 may be a candidate for such a gene...
  26. ncbi The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila
    D Gatfield
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Curr Biol 11:1716-21. 2001
    ..We conclude that HEL is essential for the export of bulk mRNA in Drosophila. The association of human UAP56 with spliced mRNAs suggests that this protein might provide a functional link between splicing and export...
  27. ncbi Chaperone suppression of alpha-synuclein toxicity in a Drosophila model for Parkinson's disease
    Pavan K Auluck
    Department of Neuroscience, University of Pennsylvania and University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 295:865-8. 2002
    ..We report that directed expression of the molecular chaperone Hsp70 prevented dopaminergic neuronal loss associated with alpha-synuclein in Drosophila and that interference with ..
  28. ncbi Tissue- and development-specific induction and turnover of hsp70 transcripts from loci 87A and 87C after heat shock and during recovery in Drosophila melanogaster
    S C Lakhotia
    Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India
    J Exp Biol 205:345-58. 2002
    ..genome of Drosophila melanogaster normally carries at least five nearly identical copies of heat-shock-inducible hsp70 genes, two copies at the 87A7 and three copies at the 87C1 chromosome sites...
  29. ncbi Rapid concerted evolution via gene conversion at the Drosophila hsp70 genes
    Brian R Bettencourt
    Department of Organismal Biology and Anatomy, University of Chicago, 1027 East 57th Street, Chicago, IL 60637, USA
    J Mol Evol 54:569-86. 2002
    We analyzed nucleotide variation in the hsp70 genes of Drosophila melanogaster (five genes) and D. simulans (four genes) to characterize the homogenizing and diversifying roles of gene conversion in their evolution...
  30. ncbi Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci
    Alexei Tulin
    Howard Hughes Medical Research Laboratories, Embryology Department, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA
    Science 299:560-2. 2003
    Steroid response and stress-activated genes such as hsp70 undergo puffing in Drosophila larval salivary glands, a local loosening of polytene chromatin structure associated with gene induction...
  31. pmc Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin
    Lucia Piacentini
    Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Roma, Italy
    J Cell Biol 161:707-14. 2003
    ..phenotype of intense gene activity, we did a detailed analysis of the heat shock-induced expression of the HSP70 encoding gene in larvae with different doses of HP1 and found that HP1 is positively involved in Hsp70 gene ..
  32. ncbi Larval crowding in Drosophila melanogaster induces Hsp70 expression, and leads to increased adult longevity and adult thermal stress resistance
    J G Sørensen
    Department of Genetics and Ecology, University of Aarhus, Ny Munkegade, Building 540, DK 8000, Aarhus C, Denmark
    J Insect Physiol 47:1301-1307. 2001
    In this study we show for the first time that moderate high larval density induces Hsp70 expression in Drosophila melanogaster larvae. Larval crowding led to both increased mean and maximal longevity in adults of both sexes...
  33. ncbi RNA-mediated neurodegeneration caused by the fragile X premutation rCGG repeats in Drosophila
    Peng Jin
    Department of Human Genetics, Emory University, Atlanta, GA 30322, USA
    Neuron 39:739-47. 2003
    ..Although devoid of mutant protein, this neurodegeneration exhibits neuronal inclusion bodies that are Hsp70 and ubiquitin positive. Overexpression of Hsp70 could suppress the neurodegeneration...
  34. pmc Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock
    Amber K Boehm
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Mol Cell Biol 23:7628-37. 2003
    The uninduced Drosophila hsp70 gene is poised for rapid activation...
  35. ncbi Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes
    Zhuoyu Ni
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 13:55-65. 2004
    ..Brief treatment of Drosophila cells with flavopiridol leads to a reduction in the accumulation of induced hsp70 and hsp26 RNAs...
  36. pmc Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster
    Gary N Landis
    Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 1340, USA
    Proc Natl Acad Sci U S A 101:7663-8. 2004
    ..Immune reporter expression in young flies was partially predictive of remaining life span, suggesting their potential as biomonitors of aging...
  37. ncbi Mechanisms of Suppression of {alpha}-Synuclein Neurotoxicity by Geldanamycin in Drosophila
    Pavan K Auluck
    Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 280:2873-8. 2005
    ..The molecular chaperone Hsp70 protects cells from the deleterious effects of alpha-synuclein, indicating a potential therapeutic approach to ..
  38. pmc Genomic deletions of the Drosophila melanogaster Hsp70 genes
    Wei J Gong
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    Genetics 168:1467-76. 2004
    ..melanogaster we set out to delete the Hsp70 genes...
  39. ncbi Lifespan extension and elevated hsp gene expression in Drosophila caused by histone deacetylase inhibitors
    Yanmei Zhao
    The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, PR China
    J Exp Biol 208:697-705. 2005
    ..To further determine if hsp22 and hsp70 expression is correlated with lifespan, and if histone acetylation participates in this process, RNA levels for ..
  40. pmc Molecular characterization of Drosophila NELF
    Chwen Huey Wu
    Department of Biochemistry and Molecular Biology, Center for Gene Regulation, The Pennsylvania State University University Park, PA 16802, USA
    Nucleic Acids Res 33:1269-79. 2005
    ..to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided...
  41. ncbi A cluster of diagnostic Hsp68 amino acid sites that are identified in Drosophila from the melanogaster species group are concentrated around beta-sheet residues involved with substrate binding
    Mark Kellett
    Centre for Environmental Stress and Adaptation Research, School of Biological Sciences, Monash University, Victoria, Australia
    Genome 48:226-33. 2005
    ..When the predicted amino acid sequences are compared with available Hsp70 sequences, patterns of conservation suggest that the C-terminal region should be subdivided according to ..
  42. pmc Loss of Hsp70 in Drosophila is pleiotropic, with effects on thermotolerance, recovery from heat shock and neurodegeneration
    Wei J Gong
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    Genetics 172:275-86. 2006
    ..In Drosophila melanogaster the Hsp70 chaperone dominates the profile of protein synthesis during the heat-shock response...
  43. pmc Menin is a regulator of the stress response in Drosophila melanogaster
    Maria Papaconstantinou
    Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada L8S 4K1
    Mol Cell Biol 25:9960-72. 2005
    ..The overexpression of menin enhanced the expression of HSP70 in embryos and interfered with its down-regulation during recovery at the normal temperature...
  44. pmc Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation
    Lyubov A Lebedeva
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de Recherche Scientifique, Unite Mixte de Recherche 7104, Universite Louis Pasteur de Strasbourg, BP 10142, Illkirch, France
    Proc Natl Acad Sci U S A 102:18087-92. 2005
    The presence of general transcription factors and other coactivators at the Drosophila hsp70 gene promoter in vivo has been examined by polytene chromosome immunofluorescence and chromatin immunoprecipitation at endogenous heat-shock ..
  45. ncbi Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript
    Zhiqiang Zhang
    Department of Biochemistry and Molecular Biology, Center for Gene Regulation, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Mol Cell 21:65-74. 2006
    ..We show that Pcf11 is directly involved in termination in Drosophila. dPcf11 is concentrated at the 3' end of the hsp70 gene in cells, and depletion of dPcf11 with RNAi causes Pol II to readthrough the normal region of termination...
  46. pmc Remarkable site specificity of local transposition into the Hsp70 promoter of Drosophila melanogaster
    Victoria Y Shilova
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia
    Genetics 173:809-20. 2006
    ..have exploited a local transposition technique and Drosophila melanogaster strains with EPgy2 insertions near the Hsp70 gene cluster at 87A7 to produce numerous novel EPgy2 insertions into these Hsp70 genes...
  47. pmc The Drosophila P68 RNA helicase regulates transcriptional deactivation by promoting RNA release from chromatin
    Michael Buszczak
    Howard Hughes Laboratories Embryology Department, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genes Dev 20:977-89. 2006
    ..Full-length hsp70 mRNA accumulates in the nucleus near its sites of transcription following heat shock of p68 homozygotes, and hsp70 ..
  48. ncbi Dynamics of heat shock factor association with native gene loci in living cells
    Jie Yao
    Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Nature 442:1050-3. 2006
    ..After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time...
  49. ncbi Cdk9 is an essential kinase in Drosophila that is required for heat shock gene expression, histone methylation and elongation factor recruitment
    Joel C Eissenberg
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd, St Louis, MO 63104, USA
    Mol Genet Genomics 277:101-14. 2007
    ..b>Hsp 70 mRNA induction by heat shock was attenuated in Cdk9 knockdown larvae...
  50. ncbi Selection on knockdown performance in Drosophila melanogaster impacts thermotolerance and heat-shock response differently in females and males
    Donna G Folk
    Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA
    J Exp Biol 209:3964-73. 2006
    ..Finally, we compared temporal profiles of the combined expression of two major heat-shock proteins, HSC70 and HSP70, during heat stress among the females and males from the selected T(KD) lines...
  51. pmc Transcription elongation controls cell fate specification in the Drosophila embryo
    Xiaoling Wang
    Department of Biochemistry, Stony Brook University, Stony Brook, New York 11794, USA
    Genes Dev 21:1031-6. 2007
    ....
  52. pmc RNA polymerase stalling at developmental control genes in the Drosophila melanogaster embryo
    Julia Zeitlinger
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Genet 39:1512-6. 2007
    ..We propose that Pol II stalling facilitates rapid temporal and spatial changes in gene activity during development...
  53. pmc RNA polymerase is poised for activation across the genome
    Ginger W Muse
    Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA
    Nat Genet 39:1507-11. 2007
    ..This finding indicates a role for regulation of polymerase elongation in the transcriptional responses to dynamic environmental and developmental cues...
  54. pmc SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC
    Maria M Kurshakova
    Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
    EMBO J 26:4956-65. 2007
    ..Importantly, E(y)2 and Xmas-2 knockdown decreases the contact between the heat-shock protein 70 (hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription...
  55. pmc Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II
    Edwin R Smith
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Mol Cell Biol 28:1041-6. 2008
    ..Furthermore, heat shock induction results in the recruitment of dUTX to the hsp70 gene, like that of several other Pol II elongation factors...
  56. pmc Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70 null flies
    Brian R Bettencourt
    Department of Biological Sciences, University of Massachusetts Lowell, 1 University Avenue, Lowell, MA 01854, USA
    BMC Biol 6:5. 2008
    The heat shock protein Hsp70 promotes inducible thermotolerance in nearly every organism examined to date...
  57. pmc Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci
    Steven J Petesch
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Cell 134:74-84. 2008
    ..Many genes, including Drosophila melanogaster Hsp70, undergo changes in chromatin structure upon activation...
  58. pmc Contrasting patterns of transposable element insertions in Drosophila heat-shock promoters
    Robert A Haney
    Department of Organismal Biology and Anatomy, University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 4:e8486. 2009
    ..melanogaster natural populations. Hsp70 promoters exhibit more TE insertions per promoter than all other genesets in the 12 species, similarly to in ..
  59. pmc Multifunctional factor ENY2 is associated with the THO complex and promotes its recruitment onto nascent mRNA
    Daria V Kopytova
    Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334 Russia
    Genes Dev 24:86-96. 2010
    ..ENY2 and THO arrive on the transcribed region of the hsp70 gene after its activation, and ENY2 plays an important role in THO recruitment...
  60. pmc Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila
    Sibo Tian
    Department of Organismal Biology and Anatomy, University of Chicago, Chicago, Illinois, United States of America
    PLoS ONE 5:e10669. 2010
    ..In contrast, in the multi-copy gene Hsp70, the number of HSEs is nearly constant across species...
  61. pmc Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells
    Katie L Zobeck
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 40:965-75. 2010
    ..to examine at high temporal resolution the recruitment and dynamics of transcription factors to the inducible Hsp70 loci in individual Drosophila salivary gland nuclei...
  62. pmc The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization
    Stefano Morettini
    Division of Molecular Biology, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020 Innsbruck, Austria
    Nucleic Acids Res 39:3103-15. 2011
    ..II localization and H3K4 methylation but loss of CHD1 decreases transcription-induced histone eviction at the Hsp70 gene in vivo...
  63. pmc Stress-induced PARP activation mediates recruitment of Drosophila Mi-2 to promote heat shock gene expression
    Magdalena Murawska
    Institute of Tumor Research and Molecular Biology, Philipps University, Marburg, Germany
    PLoS Genet 7:e1002206. 2011
    ..We suggest that stress-induced chromatin PARylation serves to rapidly attract factors that are required for an efficient and timely transcriptional response...
  64. pmc Multiple functions of Drosophila heat shock transcription factor in vivo
    P Jedlicka
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    EMBO J 16:2452-62. 1997
    ....
  65. ncbi Aging-specific expression of Drosophila hsp22
    V King
    Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California, 90089 1340, USA
    Dev Biol 207:107-18. 1999
    ..Aging-specific induction of hsp22 is discussed with regard to current evolutionary theories of aging...
  66. ncbi Hsp70 duplication in the Drosophila melanogaster species group: how and when did two become five?
    B R Bettencourt
    Department of Organismal Biology and Anatomy, University of Chicago, Illinois 60637, USA
    Mol Biol Evol 18:1272-82. 2001
    To determine how the modern copy number (5) of hsp70 genes in Drosophila melanogaster evolved, we localized the duplication events that created the genes in the phylogeny of the melanogaster group, examined D...
  67. ncbi A Drosophila melanogaster strain from sub-equatorial Africa has exceptional thermotolerance but decreased Hsp70 expression
    O G Zatsepina
    Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 117984 Moscow, Russia
    J Exp Biol 204:1869-81. 2001
    ..of high temperatures, which in Drosophila melanogaster is due in part to the inducible molecular chaperone Hsp70, is only modest in this strain...
  68. pmc NXF1/p15 heterodimers are essential for mRNA nuclear export in Drosophila
    A Herold
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 7:1768-80. 2001
    ..We conclude that Dm NXF1/p15 heterodimers only (but not NXF2-NXF4) mediate the export of the majority of mRNAs in Drosophila cells and that the other members of the NXF family play more specialized or different roles...
  69. ncbi Drosophila wnt-1 undergoes a hydrophobic modification and is targeted to lipid rafts, a process that requires porcupine
    Linda Zhai
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 279:33220-7. 2004
    ..Based on these results we propose a model whereby lipidation targets Wnt-1 to secretory vesicles that deliver the ligand to specialized microdomains at the cell surface where it can be packaged for secretion...
  70. pmc Location of P element insertions in the proximal promoter region of Hsp70A is consequential for gene expression and correlated with fecundity in Drosophila melanogaster
    Bing Chen
    Department of Organismal Biology and Anatomy, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA
    Cell Stress Chaperones 13:11-7. 2008
    ..strains with P element insertions from -28 to -144 nucleotides 5' to the transcription start site of the Hsp70A genes-corresponding to the range of naturally occurring P element insertion sites-to elucidate the consequences of ..
  71. ncbi Promoter melting and TFIID complexes on Drosophila genes in vivo
    C Giardina
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Genes Dev 6:2190-200. 1992
    ..Melted DNA helices are found associated with the pause site of the uninduced hsp70 and hsp26 heat shock genes and the constitutively expressed beta-1 tubulin gene...
  72. ncbi Heat shock response in Drosophila
    D Pauli
    Département de Zoologie et Biologie Animale, Universite de Geneve, Chêne Bougeries, Switzerland
    Experientia 48:623-9. 1992
    ..Several of the most important aspects of the regulation of the heat shock response and of the functions of the heat shock proteins have been unraveled in Drosophila...
  73. ncbi Distribution of GAGA protein on Drosophila genes in vivo
    T O'BRIEN
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 9:1098-110. 1995
    ..GAGA protein has been implicated in the transcription of numerous Drosophila genes, including hsp70, hsp26, actin 5C, and Ubx...
  74. pmc Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo
    S K Rabindran
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892
    Mol Cell Biol 14:6552-60. 1994
    ..In addition, elevated concentrations of hsp70 alone could not significantly inhibit induction of the DNA-binding activity of endogenous HSF in cultured rat ..
  75. ncbi Heat shock factor and the heat shock response
    P K Sorger
    Department of Microbiology and Immunology, University of California, San Francisco 94143 0502
    Cell 65:363-6. 1991
  76. ncbi Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments
    M Ashburner
    Chromosoma 31:356-76. 1970
  77. ncbi Two protein-binding sites in chromatin implicated in the activation of heat-shock genes
    C Wu
    Nature 309:229-34. 1984
    ..The pattern of resistance before and after induction of gene expression suggests that heat-shock genes are activated by the sequential binding of at least two protein factors...
  78. ncbi The use of promoter fusions in Drosophila genetics: isolation of mutations affecting the heat shock response
    J J Bonner
    Cell 37:979-91. 1984
    We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies...
  79. pmc Mutations in calphotin, the gene encoding a Drosophila photoreceptor cell-specific calcium-binding protein, reveal roles in cellular morphogenesis and survival
    Y Yang
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021
    Genetics 138:413-21. 1994
    ..Analysis of these mutations suggest that Calphotin plays important roles in both rhabdomere development and in photoreceptor cell survival...
  80. ncbi Chromosomal homologies between Drosophila lebanonensis and D. melanogaster determined by in situ hybridization
    M Papaceit
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Spain
    Chromosoma 102:361-8. 1993
    ..Six probes correspond to known genes from D. melanogaster (RpII215, H3-H4, MHC, hsp28/23, hsp83, hsp70), four probes are clones isolated from a D...
  81. ncbi Transcriptional regulation in Drosophila during heat shock: a nuclear run-on analysis
    J Vazquez
    Departement de Biologie Moleculaire, Universite de Geneve, Switzerland
    Chromosoma 102:233-48. 1993
    ..2 polymerases per gene). Engaged but paused RNA polymerase molecules were found at the hsp70 and hsp26 genes, but not at the other heat shock genes...
  82. ncbi Cell-specific expression and heat-shock induction of Hsps during spermatogenesis in Drosophila melanogaster
    S Michaud
    CHUL Research Center CHUQ, Universite Laval, Ste Foy, Quebec, Canada
    J Cell Sci 110:1989-97. 1997
    ..However other Hsps such as Hsp70 and Hsp22 were induced under heat-shock conditions in testes...
  83. ncbi Comparative analysis of promoters for transient gene expression in cultured mosquito cells
    Y G Zhao
    School of Biological Sciences, University of Liverpool, Donnan Laboratories
    Insect Mol Biol 8:31-8. 1999
    Three heterologous promoters (hsp70 and actin 5C from Drosophila melanogaster and IE1 from the immediate early gene of the Bombyx mori baculovirus) were assessed for their ability to drive transient luciferase expression in mosquito ..
  84. pmc Co-operative DNA binding by GAGA transcription factor requires the conserved BTB/POZ domain and reorganizes promoter topology
    K R Katsani
    Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 18:698-708. 1999
    ..We suggest a model in which the promoter DNA is wrapped around a GAGA multimer in a conformation that may exclude normal nucleosome formation...
  85. ncbi Are scs and scs' 'neutral' chromatin domain boundaries of the locus?
    Z Avramova
    Trends Genet 15:138-9. 1999
  86. pmc Regulation of heat shock proteins, Hsp70 and Hsp64, in heat-shocked Malpighian tubules of Drosophila melanogaster larvae
    Subhash C Lakhotia
    Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, Uttar Pradesh, India
    Cell Stress Chaperones 7:347-56. 2002
    ..in Malpighian tubules (MTs) of Drosophila larvae is different from that in other tissues because instead of the Hsp70 and other common heat shock proteins, Hsp64 and certain other new proteins are induced immediately after HS...
  87. ncbi Heat-induced expression of a molecular chaperone decreases by selecting for long-lived individuals
    Fabian M Norry
    Department of Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg 540, DK 8000, Aarhus C, Denmark
    Exp Gerontol 38:673-81. 2003
    The 70 kDa heat-shock protein (Hsp70) exhibits a broad range of chaperone functions that respond both to internal and external stresses, and its heat-induced expression both declines with age and reduces age-specific mortality rates...
  88. pmc Asymmetric sequence divergence of duplicate genes
    Gavin C Conant
    Department of Biology, The University of New Mexico, Albuquerque, New Mexico 87131, USA
    Genome Res 13:2052-8. 2003
    ..The method is also more sensitive in detecting positive selection (Ka/Ks > 1) than models relying only on pairwise gene comparisons...
  89. pmc Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo
    Brian E Schwartz
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA
    Mol Cell Biol 23:6876-86. 2003
    ..The requirement for Cdk7 occurs very early in the transcription cycle. Furthermore, we provide evidence that TFIIH associates with the elongation complex much longer than previously suspected...
  90. ncbi Genetics of aging in the fruit fly, Drosophila melanogaster
    Stephen L Helfand
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3301, USA
    Annu Rev Genet 37:329-48. 2003
    ....
  91. ncbi A constitutively active cryptochrome in Drosophila melanogaster
    Stephane Dissel
    Department of Biology, University of Leicester, University Road, Leicester, LE1 7RH, UK
    Nat Neurosci 7:834-40. 2004
    ..These effects are similar to those seen in wild-type flies under continuous light and suggest a regulatory role for the C terminus of CRY on the photosensitive, photolyase-like part of the protein...
  92. ncbi Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS
    Karen Adelman
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 17:103-12. 2005
    ..we report that Pol II is susceptible to transcription arrest within the promoter-proximal region of Drosophila hsp70 and that transcript cleavage factor TFIIS is essential for rapid induction of hsp70 RNA...
  93. pmc The hangover gene defines a stress pathway required for ethanol tolerance development
    Henrike Scholz
    Department of Anatomy, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    Nature 436:845-7. 2005
    ..There is growing recognition that stress, at both the cellular and systemic levels, contributes to drug- and addiction-related behaviours in mammals. Our studies suggest that this role may be conserved across evolution...
  94. ncbi Evaluation of the activities of the medfly and Drosophila hsp70 promoters in vivo in germ-line transformed medflies
    K Kalosaka
    Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, 26 500 Patras, Greece
    Insect Mol Biol 15:373-82. 2006
    The promoter of the hsp70 gene of Drosophila melanogaster has been widely used for the expression of foreign genes in other insects...
  95. ncbi Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation
    Jie Yao
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 28:978-90. 2007
    ..an "efficient entry" mode, which is followed by the progressive establishment of transcription "compartments" at Hsp70 loci where concentrated Pol II is used in a "local recycling" mode...
  96. pmc Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells
    Kazuko Hanyu-Nakamura
    Laboratory for Germline Development, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Nature 451:730-3. 2008
    ..Thus, inhibition of P-TEFb is probably a common mechanism during germ cell specification in the disparate organisms C. elegans and Drosophila...
  97. doi The RNA Pol II CTD phosphatase Fcp1 is essential for normal development in Drosophila melanogaster
    István Tombácz
    Institute of Biochemistry, Biological Research Center, Szeged, Hungary
    Gene 446:58-67. 2009
    ..The in vivo experimental system described here suggests that fly Fcp1 is associated with the transcription engaged Pol II and offers versatile possibilities for studying this evolutionary conserved essential enzyme...
  98. doi Physical assessment of toxicology at nanoscale: nano dose-metrics and toxicity factor
    P P Pompa
    Center for Bio Molecular Nanotechnology, Italian Institute of Technology, Via Barsanti 1, 73010 Arnesano, Lecce, Italy
    Nanoscale 3:2889-97. 2011
    ..This approach may pave the way to a systematic classification of nanomaterials, leading to important developments in risk assessment and regulatory approval, as well as in a wide range of nanomedicine applications...
  99. pmc Mutation generating a fragment of the major heat shock-inducible polypeptide in Drosophila melanogaster
    C Caggese
    Proc Natl Acad Sci U S A 76:2385-9. 1979
    ..One break point of the deletion is in subdivision 87A, close to or at a heat shock locus that codes for the 70,000-Dal protein...
  100. ncbi Studies of cloned sequences from four Drosophila heat shock loci
    R Holmgren
    Cell 18:1359-70. 1979
    ..in an interval of a few hundred bp near the 5' end of the messenger sequence, and the corresponding two classes of hsp 70 messenger RNA are found on polysomes after heat shock...
  101. ncbi Messenger RNA in heat-shocked Drosophila cells
    A Spradling
    J Mol Biol 109:559-87. 1977

Research Grants12

  1. 2003 Gordon Research Conference - Biology of Aging
    John Tower; Fiscal Year: 2003
    ..Significant funding is also provided by the Gordon Research Conference Organization, and additional funds are being solicited from private foundations and industry. ..
  2. DROSOPHILA LONGEVITY ASSURANCE GENES
    John Tower; Fiscal Year: 2003
    ..PdL creates dominant conditional mutations, which should greatly facilitate the identification, characterization and cloning of novel Drosophila LAGs. ..
  3. Drosophila DNA Replication Origins
    John Tower; Fiscal Year: 2005
    ..The experiments will test a number of specific hypotheses as to the organization and regulation of the chorion locus DNA replication origins. ..
  4. Genetic Architecture of Hsp90-buffered variation
    Suzannah Rutherford; Fiscal Year: 2009
    ..We expect this research will identify conserved Hsp90 target pathways and an extended network of naturally polymorphic genes that influence their function. ..
  5. ACTIVITY OF THE ALZHEIMERS DISEASE PRESENIL IN PROTEIN
    Nancy Bonini; Fiscal Year: 2004
    ..The studies proposed here will clarify the biochemical activity of Presenilin in the processing of Notch, APP and other proteins, and may ultimately increase our understanding of the molecular causes of Alzheimer's disease. ..
  6. Molecular Genetic Analysis of Neurodegeneration
    Nancy Bonini; Fiscal Year: 2007
    ....
  7. IN VIVO ROLE OF PROTEOGLYCANS IN GROWTH FACTOR SIGNALING
    J Marsh; Fiscal Year: 2001
    ..Further, these in vivo studies may help in understanding the basis of some dysmorphologies in humans. ..
  8. LONGEVITY/CANDIDATE GENE POLYMORPHISMS IN DROSOPHILA
    Marc Tatar; Fiscal Year: 2003
    ..Nucleotide polymorphisms within the genes hsp70 and Cu/ZnSOD will be discovered from a sample of 100 third chromosomes extracted from a wild population...
  9. MOLECULAR BASIS OF DEVELOPMENTAL CELL INTERACTIONS
    J Marsh; Fiscal Year: 2005
    ..Finally, we have identified a collection of mutants affecting disc growth patterns that serve as an enriched collection of new candidate genes to participate in these events. ..
  10. Regulatory systems of Drosophila aging
    Marc Tatar; Fiscal Year: 2007
    ..In a comprehensive experimental design, we shall assess qualifies of stress resistance, central metabolism and mitochondria function to determine which feature best predicts variance in life span [unreadable] [unreadable]..
  11. Genetics of Reproductive Senescence
    Marc Tatar; Fiscal Year: 2010
    ..Coupled with the program-wide multidimensional measures of somatic aging, we shall evaluate the extent that reproductive and somatic aging are concordant or independent. ..
  12. Functions and Interactions dFOXO in Drosopila Aging
    Marc Tatar; Fiscal Year: 2010
    ....