Hsf

Summary

Gene Symbol: Hsf
Description: Heat shock factor
Alias: CG5748, D-HSF, Dm-Hsf, DmHSF, DmHSFa, Dmel\CG5748, HSF, HSF1, HSFa, Hsf1, dHSF, dHSFa, hsf, l(2)03091, CG5748 gene product from transcript CG5748-RA, CG5748-PA, CG5748-PB, CG5748-PC, CG5748-PD, Heat-Shock factor, Hsf-PA, Hsf-PB, Hsf-PC, Hsf-PD, heat shock factor, heat shock transcription factor, heat shock transcription factor 1, heat-shock transcription factor
Species: fruit fly

Top Publications

  1. pmc Heat shock transcription factor 1-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperones
    Nobuhiro Fujikake
    Division of Clinical Genetics, Department of Medical Genetics, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 283:26188-97. 2008
  2. ncbi Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    J Biol Chem 281:38675-81. 2006
  3. pmc The C-terminal region of Drosophila heat shock factor (HSF) contains a constitutively functional transactivation domain
    J Wisniewski
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 24:367-74. 1996
  4. pmc Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions
    M Fernandes
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Nucleic Acids Res 22:167-73. 1994
  5. ncbi Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS
    Karen Adelman
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 17:103-12. 2005
  6. ncbi Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes
    Zhuoyu Ni
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 13:55-65. 2004
  7. ncbi Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex
    Sheryl T Smith
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Nat Cell Biol 6:162-7. 2004
  8. pmc Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock
    Amber K Boehm
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Mol Cell Biol 23:7628-37. 2003
  9. ncbi Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo
    Abbie Saunders
    Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA
    Science 301:1094-6. 2003
  10. ncbi Cooperative binding of Drosophila heat shock factor to arrays of a conserved 5 bp unit
    H Xiao
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Cell 64:585-93. 1991

Research Grants

Patents

  1. Procedure for the detection and identification of viral and subviral pathogens

Scientific Experts

  • Hafid Laayouni
  • Marta L Wayne
  • R Kellum
  • JOHN THOMAS LIS
  • Morten Muhlig Nielsen
  • Alexey Moskalev
  • Denis C Bauer
  • D N Lerman
  • L Nover
  • Erik D Andrulis
  • Louise Toft Jensen
  • Jie Yao
  • Michael J Guertin
  • C Wu
  • Zhuoyu Ni
  • Abbie Saunders
  • Watt W Webb
  • Nobuhiro Fujikake
  • M Zhong
  • Katie L Zobeck
  • Janis Werner
  • Tomás Stetina
  • Bhavana G Achary
  • Yousuf O Ali
  • Sarah E Gonsalves
  • Yasuaki Enoki
  • Brian E Schwartz
  • Sarah Chahine
  • Yu Chien Chen
  • J Wisniewski
  • J Timothy Westwood
  • Chih Chiang Chan
  • Steven J Petesch
  • Weili Cai
  • M Soledad Ivaldi
  • Yoshitaka Nagai
  • Tatsushi Toda
  • Masamitsu Yamaguchi
  • H Akiko Popiel
  • Nathan A Baird
  • A Orosz
  • Zdenek Berger
  • Xiaoching Zhao
  • Scott J Neal
  • Abraham Korol
  • H R Jensen
  • Mihiro Yano
  • Jose Ramon Suarez
  • Pavan K Auluck
  • Yanmei Zhao
  • Lyubov A Lebedeva
  • Karen Adelman
  • Sheryl T Smith
  • Amber K Boehm
  • Lucia Piacentini
  • Jaroslava Korbelová
  • Vladimír Kostál
  • David S Gilmour
  • Ivy S Co
  • Haibo Zhang
  • Katie M Campbell
  • Jennifer A Armstrong
  • R Sandaltzopoulos
  • M Fernandes
  • X Fang
  • André L Martins
  • J M Park
  • Adam Siepel
  • L S Shopland
  • J T Westwood
  • R Grace Zhai
  • Hiroshi Sakurai
  • Francois Robert
  • Zak Razak
  • Andrew Darr
  • S J Kim
  • Ryan McCormack
  • Alan M Moses
  • Warren R Zipfel
  • P Jedlicka
  • S J Nowak
  • Michael J O'Donnell
  • G Mizuguchi
  • Martin S Buckley
  • Q Wei
  • Suewei I Lin
  • Chuen Sheue Chiang
  • Ying Kuan Chen
  • R Marin
  • Gwo Jen Liaw

Detail Information

Publications99

  1. pmc Heat shock transcription factor 1-activating compounds suppress polyglutamine-induced neurodegeneration through induction of multiple molecular chaperones
    Nobuhiro Fujikake
    Division of Clinical Genetics, Department of Medical Genetics, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    J Biol Chem 283:26188-97. 2008
    ..In this study, we assessed the therapeutic effects of heat shock transcription factor 1 (HSF1)-activating compounds, which induce multiple molecular chaperones, on polyQ-induced ..
  2. ncbi Induction of the heat shock pathway during hypoxia requires regulation of heat shock factor by hypoxia-inducible factor-1
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    J Biol Chem 281:38675-81. 2006
    ..Hsps are known to be regulated by heat shock factor (Hsf), but our results demonstrate an unexpected regulatory link between the oxygen-sensing and heat shock ..
  3. pmc The C-terminal region of Drosophila heat shock factor (HSF) contains a constitutively functional transactivation domain
    J Wisniewski
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nucleic Acids Res 24:367-74. 1996
    The heat shock transcription factor (HSF) is constitutively expressed in Drosophila cells as an inactive monomer...
  4. pmc Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions
    M Fernandes
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853
    Nucleic Acids Res 22:167-73. 1994
    Heat shock genes are activated by the binding of the heat shock transcription factor (HSF) to heat shock elements (HSEs), consisting of arrays of the 5-bp unit NGAAN arranged as inverted repeats...
  5. ncbi Efficient release from promoter-proximal stall sites requires transcript cleavage factor TFIIS
    Karen Adelman
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 17:103-12. 2005
    ....
  6. ncbi Coordination of transcription, RNA processing, and surveillance by P-TEFb kinase on heat shock genes
    Zhuoyu Ni
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 13:55-65. 2004
    ..We propose that P-TEFb phosphorylation of Pol II CTD coordinates transcription elongation with 3' end processing, and failure to do so leads to rapid RNA degradation...
  7. ncbi Modulation of heat shock gene expression by the TAC1 chromatin-modifying complex
    Sheryl T Smith
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Nat Cell Biol 6:162-7. 2004
    Rapid induction of the Drosophila melanogaster heat shock gene hsp70 is achieved through the binding of heat shock factor (HSF) to heat shock elements (HSEs) located upstream of the transcription start site (reviewed in ref. 3)...
  8. pmc Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock
    Amber K Boehm
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Mol Cell Biol 23:7628-37. 2003
    ..Here we examine the rapid changes upon heat shock in levels and location of heat shock factor (HSF), RNA polymerase II (Pol II) and its phosphorylated forms, and the Pol II kinase P-TEFb on hsp70 in ..
  9. ncbi Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo
    Abbie Saunders
    Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA
    Science 301:1094-6. 2003
    ..Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms...
  10. ncbi Cooperative binding of Drosophila heat shock factor to arrays of a conserved 5 bp unit
    H Xiao
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Cell 64:585-93. 1991
    Drosophila heat shock factor (HSF) exists as a multimer in solution and when bound to its regulatory element (HSE)...
  11. ncbi HSF recruitment and loss at most Drosophila heat shock loci is coordinated and depends on proximal promoter sequences
    L S Shopland
    Section of Biochemistry, Molecular and Cell Biology, 417 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Chromosoma 105:158-71. 1996
    The heat shock response in Drosophila is primarily dependent on the binding of the heat shock transcription factor, HSF, to conserved sequences in heat shock gene promoters, the heat shock elements (HSEs)...
  12. ncbi Sodium salicylate decreases intracellular ATP, induces both heat shock factor binding and chromosomal puffing, but does not induce hsp 70 gene transcription in Drosophila
    N A Winegarden
    Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario, Canada L5L 1C6
    J Biol Chem 271:26971-80. 1996
    ..Stress-induced transcription of the heat shock genes is mediated by the transcription factor known as Heat Shock Factor (HSF)...
  13. pmc Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin
    Lucia Piacentini
    Istituto Pasteur, Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Roma, Italy
    J Cell Biol 161:707-14. 2003
    ..These data significantly broaden the current views of the roles of HP1 in vivo by demonstrating that this protein has multifunctional roles...
  14. pmc Regulation of Drosophila heat shock factor trimerization: global sequence requirements and independence of nuclear localization
    A Orosz
    Laboratory of Molecular Cell Biology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    Mol Cell Biol 16:7018-30. 1996
    b>Heat shock transcription factor (HSF) is a multidomain protein that exists as a monomer under normal conditions and is reversibly induced upon heat shock to a trimeric state that binds to DNA with high affinity...
  15. pmc Proteolytic mapping of heat shock transcription factor domains
    M Zhong
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    Protein Sci 5:2592-9. 1996
    ..In this work, limited proteolysis was used as a biochemical probe of the domain organization of Drosophila HSF. Both unshocked monomeric and heat-shocked trimeric HSF possess an internal protease-sensitive region located ..
  16. ncbi Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock
    J M Park
    National Creative Research Initiative Center for Genome Regulation, Department of Biochemistry, Yonsei University, 120 749, Seoul, South Korea
    Mol Cell 8:9-19. 2001
    ..Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly recruited to HSF binding sites...
  17. pmc High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation
    E D Andrulis
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 14:2635-49. 2000
    ..Spt6 is recruited within 2 minutes of a heat shock, similar to heat shock factor (HSF); moreover, this recruitment is dependent on HSF...
  18. ncbi Cooperative and competitive protein interactions at the hsp70 promoter
    P B Mason
    Department of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 272:33227-33. 1997
    Drosophila heat shock factor (HSF) binds to specific sequence elements of heat shock genes and can activate their transcription 200-fold...
  19. ncbi Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1
    Scott J Neal
    Department of Biology, University of Toronto, Mississauga, Ontario, Canada
    Physiol Genomics 25:493-501. 2006
    ..synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain (Drosophila heat shock factor mutant hsf4), which has been reported not to be able to produce inducible Hsps in response to heat shock...
  20. pmc An RNA aptamer that interferes with the DNA binding of the HSF transcription activator
    Xiaoching Zhao
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Nucleic Acids Res 34:3755-61. 2006
    b>Heat shock factor (HSF) is a conserved and highly potent transcription activator. It is involved in a wide variety of important biological processes including the stress response and specific steps in normal development...
  21. ncbi Dynamics of heat shock factor association with native gene loci in living cells
    Jie Yao
    Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Nature 442:1050-3. 2006
    ..We show that heat shock factor (HSF), the transcription activator of hsp70, is localized to the nucleus before heat shock and translocates ..
  22. pmc P-TEFb is critical for the maturation of RNA polymerase II into productive elongation in vivo
    Zhuoyu Ni
    Department of Molecular Biology and Genetics, 1 School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA
    Mol Cell Biol 28:1161-70. 2008
    ..In the continued presence of P-TEFb inhibitor, Pol II levels across the gene eventually recovered...
  23. pmc Rapid, transcription-independent loss of nucleosomes over a large chromatin domain at Hsp70 loci
    Steven J Petesch
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Cell 134:74-84. 2008
    ..An RNAi screen of 28 transcription and chromatin-related factors reveals that depletion of heat shock factor, GAGA Factor, or Poly(ADP)-Ribose Polymerase or its activity abolishes the loss of nucleosomes upon Hsp70 ..
  24. ncbi Stress-induced oligomerization and chromosomal relocalization of heat-shock factor
    J T Westwood
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Nature 353:822-7. 1991
    The induction of heat-shock transcription factor (HSF) binding to DNA is accomplished by a heat-induced oligomerization...
  25. ncbi DNA sequence requirements for generating paused polymerase at the start of hsp70
    H Lee
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    Genes Dev 6:284-95. 1992
    ..However, a connection between transcription and pausing is seen. The level of paused polymerase on the various hybrid hsp70-yp1 promoters correlates with the promoter's potential to direct heat-induced transcription...
  26. pmc Whole-genome analysis reveals that active heat shock factor binding sites are mostly associated with non-heat shock genes in Drosophila melanogaster
    Sarah E Gonsalves
    Department of Cell and Systems Biology, University of Toronto, Mississauga, Canada
    PLoS ONE 6:e15934. 2011
    ..HS) and other stresses, HS gene transcription in eukaryotes is up-regulated by the transcription factor heat shock factor (HSF)...
  27. doi Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms
    Yasuaki Enoki
    Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan
    FEBS Lett 585:1293-8. 2011
    b>Heat shock transcription factor (HSF), an evolutionarily conserved heat-responsive regulator, binds to heat shock elements (HSEs) comprising continuous inverted repeats of the pentamer nGAAn...
  28. pmc P-TEFb kinase recruitment and function at heat shock loci
    J T Lis
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 14:792-803. 2000
    ..Upon heat shock, P-TEFb, like the regulatory factor HSF, is rapidly recruited to heat shock loci, and this recruitment is blocked in an HSF mutant...
  29. pmc Multiple functions of Drosophila heat shock transcription factor in vivo
    P Jedlicka
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4255, USA
    EMBO J 16:2452-62. 1997
    b>Heat shock transcription factor (HSF) is a transcriptional activator of heat shock protein (hsp) genes in eukaryotes...
  30. ncbi Sensitivity of Drosophila heat shock transcription factor to low pH
    M Zhong
    Laboratory of Molecular Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Biol Chem 274:3135-40. 1999
    The heat shock transcription factor (HSF) mediates the induction of heat shock gene expression...
  31. pmc The Role of Inducible Hsp70, and Other Heat Shock Proteins, in Adaptive Complex of Cold Tolerance of the Fruit Fly (Drosophila melanogaster)
    Tomás Stetina
    Institute of Entomology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic
    PLoS ONE 10:e0128976. 2015
    ..melanogaster: the wild-type and the Hsp70- null mutant lacking all six copies of Hsp70 gene...
  32. pmc RNAi screen in Drosophila larvae identifies histone deacetylase 3 as a positive regulator of the hsp70 heat shock gene expression during heat shock
    Bhavana G Achary
    Department of Biochemistry and Molecular Biology, Center for Eukaryotic Gene Regulation, Pennsylvania State University, University Park, PA 16802, USA
    Biochim Biophys Acta 1839:355-63. 2014
    ..We validated the screen by showing that the depletion of HSF, CycT, Cdk9, Nurf 301, or ELL prevented the full induction of hsp70 by heat shock...
  33. pmc Dual-functioning transcription factors in the developmental gene network of Drosophila melanogaster
    Denis C Bauer
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072 Australia
    BMC Bioinformatics 11:366. 2010
    ..However, all of the models to date assume a transcription factor (TF) to have either activating or repressing function towards all the genes it is regulating...
  34. pmc The Torso signaling pathway modulates a dual transcriptional switch to regulate tailless expression
    Yu Chien Chen
    Department of Life Sciences and Institute of Genome Sciences, National Yang Ming University, Taipei, 112 Taiwan, ROC
    Nucleic Acids Res 37:1061-72. 2009
    ..In this study, an expanded tll expression pattern was shown in embryos with reduced heat shock factor (hsf) and Trithorax-like (Trl) activities...
  35. pmc Imaging transcription dynamics at endogenous genes in living Drosophila tissues
    Jie Yao
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Methods 45:233-41. 2008
    ..This method has provided the experimental capability to visualize the assembly and dynamics of individual transcription factors and regulators and to dissect their functions at their endogenous gene targets in living cells...
  36. ncbi Histone acetylation regulates both transcription initiation and elongation of hsp22 gene in Drosophila
    Yanmei Zhao
    The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, PR China
    Biochem Biophys Res Commun 326:811-6. 2005
    ..Meanwhile, the elevated acetylation level increased the accessibility of heat shock factor to target cis-acting regulatory sites...
  37. ncbi Deleterious and protective properties of an aggregate-prone protein with a polyalanine expansion
    Zdenek Berger
    Department of Medical Genetics, University of Cambridge, Cambridge, UK
    Hum Mol Genet 15:453-65. 2006
    ..Thus, overexpression of an aggregate-prone protein without any normal functions can result in both pathogenic and protective effects in cell culture and in vivo...
  38. pmc Identification of chromosomal regions involved in decapentaplegic function in Drosophila
    R E Nicholls
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 149:203-15. 1998
    ..As neither of these regions has been previously implicated in dpp function, we propose that each of the deficiencies removes a novel factor or factors required for dpp function...
  39. ncbi Protein traffic on the heat shock promoter: parking, stalling, and trucking along
    J Lis
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14850
    Cell 74:1-4. 1993
  40. pmc Chromatin remodeling by GAGA factor and heat shock factor at the hypersensitive Drosophila hsp26 promoter in vitro
    G Wall
    European Molecular Biology Laboratory, Gene Expression Programme, Heidelberg, Germany
    EMBO J 14:1727-36. 1995
    ..The interactions of GAGA factor and heat shock factor with their binding sites in chromatin occurred in two modes...
  41. pmc Accurate prediction of inducible transcription factor binding intensities in vivo
    Michael J Guertin
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
    PLoS Genet 8:e1002610. 2012
    ..We applied our methods to the Drosophila Heat Shock Factor (HSF), which inducibly binds a target DNA sequence element (HSE) following heat shock stress...
  42. doi Effects of acute or chronic exposure to dietary organic anions on secretion of methotrexate and salicylate by Malpighian tubules of Drosophila melanogaster larvae
    Sarah Chahine
    Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada
    Arch Insect Biochem Physiol 73:128-47. 2010
    ..Increased fluid secretion may enhance secretion of organic anions by eliminating diffusive backflux from the tubule lumen to the hemolymph...
  43. pmc Thermal evolution of gene expression profiles in Drosophila subobscura
    Hafid Laayouni
    Departament de Genetica i de Microbiologia, Grup de Biologia Evolutiva, Universitat Autonoma de Barcelona, Bellaterra Barcelona, Spain
    BMC Evol Biol 7:42. 2007
    ..Total mRNA from each population was compared to a reference pool mRNA in a standard, highly replicated two-colour competitive hybridization experiment using cDNA microarrays...
  44. ncbi Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation
    Jie Yao
    School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 28:978-90. 2007
    ..Pol II at highly transcribed developmental loci exhibits dynamics resembling combinations of these Hsp70 transcription modes...
  45. ncbi HSF access to heat shock elements in vivo depends critically on promoter architecture defined by GAGA factor, TFIID, and RNA polymerase II binding sites
    L S Shopland
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Genes Dev 9:2756-69. 1995
    ..of the Drosophila hsp70 promoter for their ability to facilitate the binding of the transcription factor, heat shock factor (HSF), to chromatin...
  46. ncbi The effect of a synergistic concentration of a Piper nigrum extract used in conjunction with pyrethrum upon gene expression in Drosophila melanogaster
    H R Jensen
    Department of Biology, University of Ottawa, Center for Advanced Research in Environmental Genomics, Ottawa, Ontario, Canada
    Insect Mol Biol 15:329-39. 2006
    ..melanogaster treated with at least two of the following treatments: P. nigrum, pyrethrum or P. nigrum plus pyrethrum. These are likely implicated in Drosophila defence responses to toxins...
  47. pmc Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo
    S K Rabindran
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892
    Mol Cell Biol 14:6552-60. 1994
    ..It has been proposed that the DNA-binding and oligomeric state of the heat shock transcription factor (HSF) is a principal step in the induction pathway that is responsive to the level of 70-kDa stress ..
  48. pmc A novel function of 14-3-3 protein: 14-3-3zeta is a heat-shock-related molecular chaperone that dissolves thermal-aggregated proteins
    Mihiro Yano
    Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770 8503, Japan
    Mol Biol Cell 17:4769-79. 2006
    ..cells, the 14-3-3zeta is up-regulated under heat stress conditions, a process mediated by a heat shock transcription factor. As the biological action linked to heat stress, 14-3-3zeta interacted with apocytochrome c, a ..
  49. ncbi Nonradioactive, solid-phase DNase I footprints analyzed on an A.L.F. DNA Sequencer
    R Sandaltzopoulos
    European Molecular Biology Laboratory, Biochemical Instrumentation Programme, Heidelberg, FRG
    Biotechniques 17:474, 476, 478. 1994
    ..L.F.) DNA Sequencer. The combination of solid-phase footprinting technology and fluorescence-based nonradioactive detection of fragments has unique advantages over established footprinting technologies...
  50. pmc Drosophila flies in "Evolution Canyon" as a model for incipient sympatric speciation
    Abraham Korol
    Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
    Proc Natl Acad Sci U S A 103:18184-9. 2006
    ..We suggest that Drosophila populations at EC represent a rare example, demonstrating how selection overrides migration, and propose an ad hoc ecological model of incipient sympatric divergence...
  51. pmc Phosphorylation of histone H3 at Ser10 facilitates RNA polymerase II release from promoter-proximal pausing in Drosophila
    M Soledad Ivaldi
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genes Dev 21:2818-31. 2007
    ..Taken together, the results introduce H3S10 phosphorylation by JIL-1 as a hallmark of early transcription elongation in Drosophila...
  52. pmc Drosophila Naked cuticle (Nkd) engages the nuclear import adaptor Importin-alpha3 to antagonize Wnt/beta-catenin signaling
    Chih Chiang Chan
    Laboratory of Molecular Pathology, Department of Pathology, UT Southwestern Medical Center, Dallas, TX 75390 9072, USA
    Dev Biol 318:17-28. 2008
    ..The Nkd NLS is similar to the Importin-alpha3-binding NLS in the Drosophila heat-shock transcription factor (dHSF), and each Importin-alpha3-binding NLS required intact basic residues in similar positions for nuclear import and ..
  53. doi Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps
    A Moskalev
    Department of Radioecology, Institute of Biology, Komi Science Center, Syktyvkar, Russia
    Biogerontology 10:3-11. 2009
    ..and/or paraquat treatment in Drosophila in wild type strain Canton-S and strains with mutations of heat shock factor (1-4 alleles) and heat shock proteins (Hsp70Ba ( 304 ), Hsp83 ( e6A ), Hsp22 ( EY09909 ), Hsp67Bb ( EY099099 ..
  54. ncbi Molecular characterisation of the Polycomblike gene of Drosophila melanogaster, a trans-acting negative regulator of homeotic gene expression
    A Lonie
    Department of Biochemistry, University of Adelaide, Australia
    Development 120:2629-36. 1994
    ..These data add further support for a model in which Polycomb Group proteins form multimeric protein complexes at specific chromosomal loci to repress transcription at those loci...
  55. pmc RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila
    Weili Cai
    Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    Development 135:2917-25. 2008
    ....
  56. pmc New candidate genes for heat resistance in Drosophila melanogaster are regulated by HSF
    Louise Toft Jensen
    Department of Biological Sciences, University of Aarhus, Ny Munkegade, Aarhus C, Denmark
    Cell Stress Chaperones 13:177-82. 2008
    ..Hsps are all regulated by a common transcription factor, the heat shock factor (HSF), and it is known that HSF is controlling other, so far uncharacterised, heat-responsive genes...
  57. ncbi Differences in DNA sequence recognition by the heat-shock factors of Drosophila melanogaster and the parasitic helminth Schistosoma mansoni
    R Levy-Holtzman
    Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel
    Biochim Biophys Acta 1264:134-40. 1995
    ..The interactions of HSF in Sm extracts (SmHSF) and HSF of Dm (DmHSF) with 32P-labeled heat shock element (HSE) probes, with and without unlabeled competitor DNA probes (HSE-related ..
  58. pmc Chromatin landscape dictates HSF binding to target DNA elements
    Michael J Guertin
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
    PLoS Genet 6:e1001114. 2010
    ..We used the ChIP-seq assay to examine the genome-wide distribution of Drosophila Heat Shock Factor (HSF), a TF whose binding activity is mediated by heat shock-induced trimerization...
  59. pmc Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells
    Katie L Zobeck
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Cell 40:965-75. 2010
    ..Recruitment of the master regulator, HSF, is first detected within 20 s of gene activation; the timing of its recruitment resolves from RNA polymerase II ..
  60. ncbi Hydrogen peroxide activates immediate binding of a Drosophila factor to DNA heat-shock regulatory element in vivo and in vitro
    J Becker
    Unité Associée au Centre National de la Recherche Scientifique 1135, Universite Pierre et Marie Curie, Paris, France
    Eur J Biochem 189:553-8. 1990
    ..These results were confirmed with mouse fibroblast cells. This very rapid activation, in vivo or in vitro, suggests a direct effect of H2O2 either on the heat-shock factor itself or on its activator...
  61. pmc Nicotinamide mononucleotide adenylyltransferase is a stress response protein regulated by the heat shock factor/hypoxia-inducible factor 1alpha pathway
    Yousuf O Ali
    Department of Molecular and Cellular Pharmacology, University of Miami, Miller School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 286:19089-99. 2011
    ..is transcriptionally regulated during various stress conditions including heat shock and hypoxia through heat shock factor (HSF) and hypoxia-inducible factor 1α in vivo...
  62. ncbi Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation
    J Clos
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Cell 63:1085-97. 1990
    We report the cloning of the transcriptional activator of heat shock genes, HSF, from Drosophila...
  63. ncbi Transcription elongation and eukaryotic gene regulation
    C A Spencer
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
    Oncogene 5:777-85. 1990
    ..We will also present evidence supporting the model that modifications to the RNA polymerase II transcription complex are pivotal to the control of transcriptional at the level of elongation...
  64. ncbi Heat shock factor and the heat shock response
    P K Sorger
    Department of Microbiology and Immunology, University of California, San Francisco 94143 0502
    Cell 65:363-6. 1991
  65. pmc The Drosophila BRM complex facilitates global transcription by RNA polymerase II
    Jennifer A Armstrong
    Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA
    EMBO J 21:5245-54. 2002
    ..These data suggest that the chromatin remodeling activity of the BRM complex plays a general role in facilitating transcription by RNA polymerase II...
  66. ncbi Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin
    G Mizuguchi
    Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    Mol Cell 1:141-50. 1997
    ..Our results provide direct evidence that NURF is able to assist gene activation in a chromatin context, and identify a stage of NURF dependence early in the process leading to transcriptional initiation...
  67. ncbi Direct sensing of heat and oxidation by Drosophila heat shock transcription factor
    M Zhong
    Laboratory of Molecular Cell Biology, National Cancer Institute, Bethesda, Maryland 20892 4255, USA
    Mol Cell 2:101-8. 1998
    The heat shock transcription factor HSF activates expression of its target genes in response to elevated temperatures and chemical or physiological stress...
  68. ncbi Developmentally regulated nuclear transport of transcription factors in Drosophila embryos enable the heat shock response
    Z Wang
    Committee on Developmental Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 125:4841-50. 1998
    ..In both, regulation occurred at the level of transcription. During the refractory period for Hsp70 induction, HSF (heat-shock transcription factor) exhibited specific DNA-binding activity characteristic of activation in extracts ..
  69. ncbi Cell-specific expression and heat-shock induction of Hsps during spermatogenesis in Drosophila melanogaster
    S Michaud
    CHUL Research Center CHUQ, Universite Laval, Ste Foy, Quebec, Canada
    J Cell Sci 110:1989-97. 1997
    ..The heat shock transcriptional factor of Drosophila (DmHSF), present in significantly lower amount in testes when compared to other tissues such as the head, was shown to be ..
  70. ncbi The subcellular localization and activity of Drosophila cubitus interruptus are regulated at multiple levels
    Q T Wang
    Department of Biochemistry, Molecular Biology and Cell Biology, Robert H Lurie Comprehensive Cancer Center, Northwestern University, Evanston, IL 60208, USA
    Development 126:5097-106. 1999
    ..Finally, we propose that there are three levels of apparent Ci activity, corresponding to three zones along the AP axis with different sets of gene expression and different levels of Hh signaling...
  71. pmc Cell-specific heat-shock induction of Hsp23 in the eye of Drosophila melanogaster
    R Marin
    Centre deRecherche du CHUL, Universite Laval, Ste Foy, Quebec, Canada
    Cell Stress Chaperones 1:40-6. 1996
    ..stress conditions does not seem to be regulated by the Drosophila melanogaster heat shock transcriptional factor (DmHSF)...
  72. ncbi Laboratory selection at different temperatures modifies heat-shock transcription factor (HSF) activation in Drosophila melanogaster
    D N Lerman
    The Committee on Evolutionary Biology, The University of Chicago, Chicago, IL 60637, USA
    J Exp Biol 204:315-23. 2001
    The magnitude and time course of activation of the heat-shock transcription factor (HSF) differ among Drosophila melanogaster lines evolving at 18 degrees C, 25 degrees C or 28 degrees C for more than 20 years...
  73. ncbi Nuclear entry, oligomerization, and DNA binding of the Drosophila heat shock transcription factor are regulated by a unique nuclear localization sequence
    E Zandi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA
    Genes Dev 11:1299-314. 1997
    In normally growing Drosophila cultured cells the Drosophila heat shock transcription factor (dHSF) is localized in the cytosol and translocates into the nucleus after heat shock...
  74. pmc EZ-Retrieve: a web-server for batch retrieval of coordinate-specified human DNA sequences and underscoring putative transcription factor-binding sites
    Haibo Zhang
    Center for Applied Genomics, Public Health Research Institute, 225 Warren Street, ICPH W420M, Newark, NJ 07103, USA
    Nucleic Acids Res 30:e121. 2002
    ..A major application of EZ-Retrieve is the analysis of co-expressed genes that are highlighted as expression clusters in DNA microarray experiments...
  75. ncbi The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila
    Erik D Andrulis
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 420:837-41. 2002
    ..These data provide a physical basis for the hypothesis that exosome-mediated pre-mRNA surveillance accompanies transcription elongation...
  76. pmc Binding of heat shock factor to and transcriptional activation of heat shock genes in Drosophila
    M Fernandes
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853, USA
    Nucleic Acids Res 23:4799-804. 1995
    b>Heat shock factor (HSF) binds to heat shock elements (HSEs) and the binding can be highly cooperative. Here we report an analysis of binding of Drosophila HSF to both native and synthetic heat shock regulatory regions...
  77. pmc Cdk7 is required for full activation of Drosophila heat shock genes and RNA polymerase II phosphorylation in vivo
    Brian E Schwartz
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA
    Mol Cell Biol 23:6876-86. 2003
    ..The requirement for Cdk7 occurs very early in the transcription cycle. Furthermore, we provide evidence that TFIIH associates with the elongation complex much longer than previously suspected...
  78. ncbi Mechanisms of Suppression of {alpha}-Synuclein Neurotoxicity by Geldanamycin in Drosophila
    Pavan K Auluck
    Department of Neuroscience, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 280:2873-8. 2005
    ..These studies indicate that compounds that modulate the stress response are a promising approach to treat Parkinson's disease...
  79. ncbi RNA interference demonstrates a role for nautilus in the myogenic conversion of Schneider cells by daughterless
    Q Wei
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Biol 228:239-55. 2000
    ..Genes encoding proteins with relatively short half-lives (10 h), such as nautilus or HSF, are efficiently silenced, whereas more stable proteins, such as cytoplasmic actin or beta-galactosidase, are less ..
  80. pmc Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation
    Lyubov A Lebedeva
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de Recherche Scientifique, Unite Mixte de Recherche 7104, Universite Louis Pasteur de Strasbourg, BP 10142, Illkirch, France
    Proc Natl Acad Sci U S A 102:18087-92. 2005
    ....
  81. ncbi Cooperative binding of heat shock transcription factor to the Hsp70 promoter in vivo and in vitro
    J Amin
    Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101
    J Biol Chem 269:4804-11. 1994
    ..In vitro, HSEII is a weak and HSEI a strong binding site for heat shock transcription factor HSF, and HSF binds to HSEII cooperatively...
  82. ncbi NMR evidence for similarities between the DNA-binding regions of Drosophila melanogaster heat shock factor and the helix-turn-helix and HNF-3/forkhead families of transcription factors
    G W Vuister
    Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892
    Biochemistry 33:10-6. 1994
    Heteronuclear multidimensional NMR experiments of residues 33-163 of the DNA-binding domain of Drosophila heat shock factor, dHSF(33-163), were recorded, using only 3 mg of uniformly 15N-labeled or 2 mg of uniformly 15N/13C-labeled ..
  83. ncbi Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation
    J Lis
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Cold Spring Harb Symp Quant Biol 63:347-56. 1998
  84. pmc An ATP- and hsc70-dependent oligomerization of nascent heat-shock factor (HSF) polypeptide suggests that HSF itself could be a "sensor" for the cellular stress response
    M J Schlesinger
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110
    Protein Sci 2:1356-60. 1993
  85. ncbi Heat stress transcription factors from tomato can functionally replace HSF1 in the yeast Saccharomyces cerevisiae
    O Boscheinen
    Molecular Cell Biology, Biocenter of the J W Goethe University, Frankfurt, Germany
    Mol Gen Genet 255:322-31. 1997
    The fact that yeast HSF1 is essential for survival under nonstress conditions can be used to test heterologous Hsfs for the ability to substitute for the endogenous protein...
  86. pmc Interaction of the DNA-binding domain of Drosophila heat shock factor with its cognate DNA site: a thermodynamic analysis using analytical ultracentrifugation
    S J Kim
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Protein Sci 3:1040-51. 1994
    b>Heat shock transcription factor (HSF) mediates the activation of heat shock genes by binding to its cognate sites with high affinity and specificity...
  87. ncbi Displacement of sequence-specific transcription factors from mitotic chromatin
    M A Martinez-Balbas
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 83:29-38. 1995
    ..transcription factors that bind to the human hsp70 promoter, the DNA-binding activities of three (C/EBP, GBP, and HSF1) were normal, while Sp1 showed reduced binding activity in mitotic cell extracts...
  88. pmc Is HP1 an RNA detector that functions both in repression and activation?
    Rebecca Kellum
    101 T H Morgan Building, Department of Biology, University of Kentucky, Lexington, KY 40506 0225, USA
    J Cell Biol 161:671-2. 2003
    ..Indeed, several genes located at one of these sites (cytological region 31) have increased transcript levels in mutants for HP1 (Hwang et al., 2001)...
  89. pmc Phosphorylation of histone H3 correlates with transcriptionally active loci
    S J Nowak
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genes Dev 14:3003-13. 2000
    ..experiments confirm that this change in phosphorylated H3 distribution is dependent on functional heat shock transcription factor activity...
  90. ncbi Regulation of heat shock factor trimer formation: role of a conserved leucine zipper
    S K Rabindran
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Science 259:230-4. 1993
    ..Two subregions at the carboxyl-terminal end of human HSF1 were identified that maintain the monomeric form of the protein under normal conditions...
  91. ncbi Developmental regulation of the heat shock response by nuclear transport factor karyopherin-alpha3
    X Fang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Development 128:3349-58. 2001
    ..Although the Drosophila heat shock transcription factor (dHSF) is abundant in the early embryo it does not enter the nucleus in response to heat shock...
  92. ncbi Elimination of background in footprinting analysis by template selection
    R Sandaltzopoulos
    European Molecular Biology Laboratory, Heidelberg, Germany
    Biotechniques 18:773, 776. 1995
  93. ncbi Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress
    Nobuhiro Fujikake
    Division of Clinical Genetics, Department of Medical Genetics, Osaka University Graduate School of Medicine, 2 2 B9 Yamadaoka, Suita, Osaka 565 0871, Japan
    FEBS Lett 579:3842-8. 2005
    b>Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock proteins in response to various types of stress...
  94. ncbi Purification of heat shock transcription factor of Drosophila
    M Zhong
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Methods Enzymol 274:113-9. 1996
  95. pmc Energy-dependent chromatin accessibility and nucleosome mobility in a cell-free system
    P D Varga-Weisz
    European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 14:2209-16. 1995
    ..This unprecedented demonstration of energy-dependent nucleosome mobility identifies a new principle which is likely to be fundamental to the mechanism of chromatin remodelling and the binding of regulatory proteins...
  96. pmc Activation of Drosophila heat shock factor: conformational change associated with a monomer-to-trimer transition
    J T Westwood
    Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892
    Mol Cell Biol 13:3481-6. 1993
    The induction of heat shock genes in eukaryotic cells is regulated by the transcription factor heat shock factor (HSF). Activation of HSF occurs at two independent levels, DNA binding and the acquisition of transcriptional competence...
  97. ncbi Antibody-mediated activation of Drosophila heat shock factor in vitro
    V Zimarino
    Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    Science 249:546-9. 1990
    ..Central to this activation is heat shock-inducible binding of the transcriptional activator, termed heat shock factor (HSF), to common regulatory elements, which are located upstream of all heat shock genes...

Research Grants2

  1. RNA APTAMER PROBES OF TRANSCRIPTIONAL MECHANISMS IN VIVO
    JOHN LIS; Fiscal Year: 2007
    ..The regulated in vivo expression of RNA aptamers that target disease-inducing proteins would prove to be of substantial therapeutic value. ..

Patents1

  1. Procedure for the detection and identification of viral and subviral pathogens
    Patent Number: EP0574345-A2; Date:1993-12-15