His2Av

Summary

Gene Symbol: His2Av
Description: Histone H2A variant
Alias: 5499, CG5499, Dmel\CG5499, H2A, H2A.F/Z, H2A.V, H2A.X, H2A.Z, H2AV, H2AV_DROM, H2AX, H2AZ, H2Av, H2AvD, H2a.V, H2av, H2avD, HIS, HIS2AV, HIS2AVD, His, His2, His2A, His2AV, His2AvD, HisH2Av, Hist, Hist2av, gamma-H2AV, gamma-H2Av, gamma-HIS2AV, gamma-His2Av, gammaH2AV, gammaH2Av, h2AvD, his, his2Av, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602, CG5499 gene product from transcript CG5499-RB, CG5499-PA, CG5499-PB, H2A variant, His2Av-PA, His2Av-PB, Histone-2A, Histone-2av, histone, histone 2A variant, histone H2A variant, histone H2Av, histone variant H2Av, histone2A, histone2Av, histone2AvD
Species: fruit fly

Top Publications

  1. ncbi Regions of variant histone His2AvD required for Drosophila development
    M J Clarkson
    The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory
    Nature 399:694-7. 1999
  2. pmc Drosophila has a single copy of the gene encoding a highly conserved histone H2A variant of the H2A.F/Z type
    A van Daal
    Department of Biology, Washington University, St Louis, MO 63130
    Nucleic Acids Res 16:7487-97. 1988
  3. ncbi A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior
    M Clarkson
    Department of Biochemistry, University of Adelaide, SA, Australia
    DNA Cell Biol 18:457-62. 1999
  4. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
  5. ncbi The EAST protein of drosophila controls an expandable nuclear endoskeleton
    M Wasser
    Institute of Molecular and Cell Biology, National University of Singapore
    Nat Cell Biol 2:268-75. 2000
  6. pmc The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis
    D H Parry
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 11:671-83. 2001
  7. pmc DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis
    James P Madigan
    Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201 2002, USA
    Nucleic Acids Res 30:3698-705. 2002
  8. pmc The degradation of two mitotic cyclins contributes to the timing of cytokinesis
    Arnaud Echard
    Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
    Curr Biol 13:373-83. 2003
  9. ncbi The Drosophila EAST protein associates with a nuclear remnant during mitosis and constrains chromosome mobility
    Martin Wasser
    Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609
    J Cell Sci 116:1733-43. 2003
  10. pmc Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila
    Jun Yuan Ji
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Development 131:401-11. 2004

Scientific Experts

Detail Information

Publications124 found, 100 shown here

  1. ncbi Regions of variant histone His2AvD required for Drosophila development
    M J Clarkson
    The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory
    Nature 399:694-7. 1999
    ..domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes...
  2. pmc Drosophila has a single copy of the gene encoding a highly conserved histone H2A variant of the H2A.F/Z type
    A van Daal
    Department of Biology, Washington University, St Louis, MO 63130
    Nucleic Acids Res 16:7487-97. 1988
    The Tetrahymena histone H2A variant designated hv1 is localized exclusively in the transcriptionally active macronucleus and is absent from the quiescent micronucleus (1)...
  3. ncbi A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior
    M Clarkson
    Department of Biochemistry, University of Adelaide, SA, Australia
    DNA Cell Biol 18:457-62. 1999
    ..melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria...
  4. pmc Megabase chromatin domains involved in DNA double-strand breaks in vivo
    E P Rogakou
    Laboratory of Molecular Pharmacology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 146:905-16. 1999
    ..breaks introduced into mammalian cells by ionizing radiation results in the specific phosphorylation of histone H2AX on serine residue 139, yielding a specific modified form named gamma-H2AX...
  5. ncbi The EAST protein of drosophila controls an expandable nuclear endoskeleton
    M Wasser
    Institute of Molecular and Cell Biology, National University of Singapore
    Nat Cell Biol 2:268-75. 2000
    ..We propose that EAST mediates the assembly of an expandable nuclear endoskeleton which, through alterations of its volume, can modulate the spatial arrangement of chromosomes...
  6. pmc The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis
    D H Parry
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 11:671-83. 2001
    ..We tested a recent proposal that the destruction of the different cyclins guides progress through mitosis...
  7. pmc DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis
    James P Madigan
    Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201 2002, USA
    Nucleic Acids Res 30:3698-705. 2002
    ..We demonstrate the DSB-induced phosphorylation of histone variant H2Av in Drosophila melanogaster. H2Av is a member of the H2AZ family of histone variants...
  8. pmc The degradation of two mitotic cyclins contributes to the timing of cytokinesis
    Arnaud Echard
    Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
    Curr Biol 13:373-83. 2003
    ..We examined the interface between mitotic cyclin destruction and the timing of cytokinesis...
  9. ncbi The Drosophila EAST protein associates with a nuclear remnant during mitosis and constrains chromosome mobility
    Martin Wasser
    Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609
    J Cell Sci 116:1733-43. 2003
    ..We propose that EAST constitutes a component of a nucleoskeleton that helps to constrain the mobility of chromosomes in interphase, mitosis and meiosis...
  10. pmc Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila
    Jun Yuan Ji
    Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
    Development 131:401-11. 2004
    ....
  11. pmc Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo
    Dmitry V Fyodorov
    Section of Molecular Biology, University of California at San Diego, La Jolla, CA 92093, USA
    Genes Dev 18:170-83. 2004
    ..These findings collectively indicate that ACF/CHRAC functions in the assembly of periodic nucleosome arrays that contribute to the repression of genetic activity in the eukaryotic nucleus...
  12. pmc Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in Drosophila
    Jiangtao Yu
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853 2703, USA
    J Cell Biol 164:487-92. 2004
    ..Chromosomes are highly undercondensed, particularly in the euchromatin, but nevertheless contain phosphorylated histone H3, condensin, and topoisomerase II...
  13. pmc The cohesion protein ORD is required for homologue bias during meiotic recombination
    Hayley A Webber
    Dept of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, NH 03755, USA
    J Cell Biol 164:819-29. 2004
    ..We conclude that ORD activity suppresses sister chromatid exchange and stimulates inter-homologue crossovers, thereby promoting homologue bias during meiotic recombination in Drosophila...
  14. ncbi Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
    Thomas Kusch
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Science 306:2084-7. 2004
    Phosphorylation of the human histone variant H2A.X and H2Av, its homolog in Drosophila melanogaster, occurs rapidly at sites of DNA double-strand breaks...
  15. ncbi The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1
    Matthias G Fischer
    BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
    Curr Biol 14:2019-24. 2004
    ..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
  16. pmc Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis
    Jason Stumpff
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Curr Biol 14:2143-8. 2004
    ..These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase...
  17. pmc The role of histone H2Av variant replacement and histone H4 acetylation in the establishment of Drosophila heterochromatin
    Jyothishmathi Swaminathan
    Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Genes Dev 19:65-76. 2005
    ..Here we show that the histone H2A variant of Drosophila melanogaster, H2Av, localizes to the centromeric heterochromatin, and it is recruited to an ..
  18. pmc Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus
    Sunil Jayaramaiah Raja
    Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
    Mol Cell Biol 25:6165-77. 2005
    ..Mst77F encodes a linker histone-like protein showing significant similarity to mammalian HILS1 protein...
  19. pmc Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
    Sebastian Heeger
    Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
    Genes Dev 19:2041-53. 2005
    ....
  20. pmc A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes
    Irena Ivanovska
    Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Genes Dev 19:2571-82. 2005
    ..We describe a mutation in a recently identified Histone H2A kinase, nhk-1, in Drosophila that leads to female sterility due to defects in the formation of the meiotic ..
  21. ncbi Patterning chromatin: form and function for H2A.Z variant nucleosomes
    Ryan M Raisner
    Department of Biochemistry and Biophysics, University of California, 600 16th Street, San Francisco, CA 94143 2240, USA
    Curr Opin Genet Dev 16:119-24. 2006
    Although many histone variants are specific to higher eukaryotes, the H2A variant H2A.Z has been conserved during eukaryotic evolution. Genetic studies have demonstrated roles for H2A...
  22. ncbi Regulation of higher-order chromatin structures by nucleosome-remodelling factors
    Patrick D Varga-Weisz
    Babraham Institute, Cambridge, CB2 4AT, UK
    Curr Opin Genet Dev 16:151-6. 2006
    ..At the level of single nucleosomes, they are involved in nucleosome-repositioning, altering histone-DNA interactions, disassembly of nucleosomes, and the exchange of histones with variants of different properties...
  23. ncbi Chromatin assembly: a basic recipe with various flavours
    Sophie E Polo
    Laboratory of Nuclear Dynamics and Genome Plasticity, UMR 218 CNRS Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 5, France
    Curr Opin Genet Dev 16:104-11. 2006
    Packaging of eukaryotic genomes into chromatin is a hierarchical mechanism, starting with histone deposition onto DNA to produce nucleosome arrays, which then further fold and ultimately form functional domains...
  24. pmc Onset of the DNA replication checkpoint in the early Drosophila embryo
    Justin Crest
    Department of Biology, University of Washington, Seattle, Washington 98195 1800, USA
    Genetics 175:567-84. 2007
    ..Our results suggest an antagonistic interaction between DNA replication checkpoint activation and Cdk1-CycB activity during the transition from preblastoderm to blastoderm cycles...
  25. pmc Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila females
    S Mehrotra
    Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
    PLoS Genet 2:e200. 2006
    Using an antibody against the phosphorylated form of His2Av (gamma-His2Av), we have described the time course for the series of events leading from the formation of a double-strand break (DSB) to a crossover in Drosophila female meiotic ..
  26. ncbi Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response
    Carla Klattenhoff
    Program in Molecular Medicine and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Dev Cell 12:45-55. 2007
    ..Furthermore, rasiRNA pathway mutations lead to germline-specific accumulation of gamma-H2Av foci characteristic of DNA damage...
  27. ncbi Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase
    Melina Schuh
    Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Curr Biol 17:237-43. 2007
    ..A candidate for an epigenetic mark is the centromere-specific histone H3 variant CENP-A that replaces H3 in alternating blocks of chromatin exclusively in active centromeres...
  28. ncbi Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes
    Raquel A Oliveira
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Chromosoma 116:259-74. 2007
    ..Furthermore, the rapid turnover of Barren-EGFP indicates that the mechanism by which condensin I promotes mitotic chromosome organization is inconsistent with a static scaffold model...
  29. pmc The cyclin-dependent kinase inhibitor Dacapo promotes replication licensing during Drosophila endocycles
    Amy Hong
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 26:2071-82. 2007
    ..Finally, genetic interaction studies suggest that dap functions to promote replication licensing in a subset of Drosophila mitotic cycles...
  30. ncbi Rapid effects of acute anoxia on spindle kinetochore interactions activate the mitotic spindle checkpoint
    Rahul Pandey
    Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
    J Cell Sci 120:2807-18. 2007
    ..Although involving adenosine triphosphate (ATP)-consuming kinases, the spindle checkpoint can therefore be activated by spindle damage in response to acute anoxia and protect against aneuploidies...
  31. pmc ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo
    Davide F V Corona
    Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, United States of America
    PLoS Biol 5:e232. 2007
    ..does not cause obvious defects in nucleosome assembly, but results in a significant reduction in the level of histone H1 associated with chromatin in vivo...
  32. pmc Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint
    Martha Klovstad
    Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
    PLoS Genet 4:e31. 2008
    ..In addition, Brca2 co-immunoprecipitates with the checkpoint protein Rad9, suggesting a direct role for Brca2 in the transduction of the meiotic recombination checkpoint signal...
  33. pmc Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
    Andrea Pauli
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Dev Cell 14:239-51. 2008
    ..These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa...
  34. pmc RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycle
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 2200, USA
    Curr Biol 18:245-54. 2008
    ..However, we still know very little of how individual cell-cycle events are coupled to this oscillator and how the timing of each event is controlled...
  35. pmc In vivo dynamics of Drosophila nuclear envelope components
    Katerina R Katsani
    Institut Curie, Centre de Recherche, Paris Cedex 05, France
    Mol Biol Cell 19:3652-66. 2008
    ..Surprisingly, Drosophila Nup107 shows no evidence of localization to kinetochores, despite the demonstrated importance of this association in mammalian cells...
  36. pmc PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen
    S Kotadia
    Department of Pharmacology, The Cecil and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9051, USA
    Oncogene 27:6334-46. 2008
    ..These analyses demonstrate the validity and utility of Drosophila as a model for viral oncoprotein function in vivo...
  37. pmc Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
    Anne Royou
    Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    J Cell Biol 183:63-75. 2008
    ..Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms...
  38. pmc Loss of the histone variant H2A.Z restores capping to checkpoint-defective telomeres in Drosophila
    Yikang S Rong
    Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 180:1869-75. 2008
    The conserved histone variant H2A.Z fulfills many functions by being an integral part of the nucleosomes placed at specific regions of the genome...
  39. pmc Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formation
    Eric F Joyce
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
    Genetics 181:39-51. 2009
    ..Interestingly, PCH2-dependent delays in prophase may allow additional crossovers to form...
  40. pmc Endocycling cells do not apoptose in response to DNA rereplication genotoxic stress
    Sonam Mehrotra
    Department of Biology, Syracuse University, Syracuse, New York 13244, USA
    Genes Dev 22:3158-71. 2008
    ..Similar mechanisms may operate during vertebrate development, with implications for cancer predisposition in certain tissues...
  41. pmc Genome-wide profiling of salt fractions maps physical properties of chromatin
    Steven Henikoff
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Genome Res 19:460-9. 2009
    ..3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3...
  42. pmc The Cyclin-dependent kinase inhibitor Dacapo promotes genomic stability during premeiotic S phase
    Karine Narbonne-Reveau
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 20:1960-9. 2009
    ..Finally, we report that dap(-/-) ovarian cysts frequently undergo an extramitotic division before meiotic entry, indicating that Dap influences the timing of the mitotic/meiotic transition...
  43. pmc Influence of cyclin type and dose on mitotic entry and progression in the early Drosophila embryo
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    J Cell Biol 184:639-46. 2009
    ....
  44. pmc Drosophila ISWI regulates the association of histone H1 with interphase chromosomes in vivo
    Giorgia Siriaco
    Adolf Butenandt Institute, Molecular Biology, Munich Center of Integrated Protein Science, Ludwig Maximilians University, 80336 Munich, Germany
    Genetics 182:661-9. 2009
    ..chromatin-remodeling factor plays a key role in this process by promoting the assembly of chromatin containing histone H1. To test this hypothesis, we investigated the function of H1 in Drosophila...
  45. pmc Cell rearrangements, cell divisions and cell death in a migrating epithelial sheet in the abdomen of Drosophila
    Marcus Bischoff
    Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
    Development 136:2403-11. 2009
    ..Furthermore, our results suggest that an active force created by the histoblasts contributes to the formation of the adult epidermis. Finally, we show that histoblasts occasionally undergo apoptosis...
  46. pmc Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion
    Haifeng Wang
    Department of Molecular and Cell Biology, One Shields Avenue, University of California Davis, Davis, California 95616, USA
    Cytoskeleton (Hoboken) 67:715-28. 2010
    ....
  47. doi Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1
    Ralf B Schittenhelm
    Institute of Molecular Life Sciences, University of Zurich, CH 8057 Zurich, Switzerland
    J Cell Sci 123:3768-79. 2010
    ..cycle progression in higher eukaryotes depends critically on the faithful incorporation of a centromere-specific histone H3 variant encoded by CENPA in humans and cid in Drosophila...
  48. pmc The Drosophila zinc finger protein trade embargo is required for double strand break formation in meiosis
    Cathleen M Lake
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 7:e1002005. 2011
    ..We speculate that Trem may act by either directing the binding of Mei-P22 to preferred sites of DSB formation or by altering chromatin structure in a manner that allows Mei-P22 to form foci...
  49. doi TLM-Converter: reorganization of long time-lapse microscopy datasets for downstream image analysis
    Wee Choo Puah
    Bioinformatics Institute, A STAR, Singapore
    Biotechniques 51:49-50, 52-3. 2011
    ..In addition, saving the outputs in open standard image file formats enables data sharing between independently developed software tools...
  50. pmc Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair
    Eric F Joyce
    Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 08854, USA
    J Cell Biol 195:359-67. 2011
    ..Both kinases phosphorylate H2AV (γ-H2AV), and, using this as a reporter for ATM/ATR activity, we found that the DSB repair response is ..
  51. pmc Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53
    Brent S Wells
    Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
    Dev Biol 361:263-76. 2012
    ..find that after IR, p53 is required for imaginal disc cells to repair DNA, and in its absence the damage marker, γ-H2AX is persistently expressed...
  52. doi Three-dimensional segmentation of nuclei and mitotic chromosomes for the study of cell divisions in live Drosophila embryos
    Rambabu Chinta
    Bioinformatics Institute, Agency for Science, Technology and Research A STAR, Singapore 138671, Republic of Singapore
    Cytometry A 81:52-64. 2012
    ..We tested our method on 3D time-series images of live embryos expressing histone-2Av-green fluorescence protein...
  53. ncbi Mitosis in primary cultures of Drosophila melanogaster larval neuroblasts
    Matthew S Savoian
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, P O Box 509, Albany, New York 12201 0509, USA
    J Cell Sci 115:3061-72. 2002
    ..5 minutes. Analysis of neuroblasts containing various GFP-tagged proteins (e.g. histone, fizzy, fizzy-related and alpha-tubulin) reveals that attaching kinetochores exhibit sudden, rapid pole-directed ..
  54. ncbi Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutants
    Rahul Pandey
    BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    J Cell Sci 118:733-42. 2005
    ....
  55. pmc Regulation of mitosis in response to damaged or incompletely replicated DNA require different levels of Grapes (Drosophila Chk1)
    Amanda Purdy
    MCD Biology, University of Colorado, Boulder, CO 80309 0347, USA
    J Cell Sci 118:3305-15. 2005
    ..Finally, our results demonstrate that Drosophila provides a unique opportunity to study the regulation of the entry into, and progress through, mitosis by DNA structure checkpoints in metazoa...
  56. pmc DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 187:7-14. 2009
    ..We conclude that normal S phase and replication checkpoint activities are important timers of the undisturbed cell cycle before, but not after, the MBT...
  57. pmc Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo
    Ivo A Telley
    Cell Biology and Biophysics Unit and 2 Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
    J Cell Biol 197:887-95. 2012
    ..These activities define a characteristic separation length scale that appears to be a conserved property of developing insect embryos...
  58. pmc A histone variant, H2AvD, is essential in Drosophila melanogaster
    A van Daal
    Department of Biology, Washington University, St Louis, Missouri 63130
    Mol Biol Cell 3:593-602. 1992
    H2AvD, a Drosophila melanogaster histone variant of the H2A.Z class, is encoded by a single copy gene in the 97CD region of the polytene chromosomes...
  59. ncbi Histone H2A.Z is widely but nonrandomly distributed in chromosomes of Drosophila melanogaster
    T J Leach
    Wadsworth Center, New York State Department of Health and Department of Biomedical Sciences, State University of New York, Albany, New York 12201 2002, USA
    J Biol Chem 275:23267-72. 2000
    ..H2A.F/Z is a highly conserved family of H2A variants. H2Av, the H2A...
  60. ncbi The Grapes checkpoint coordinates nuclear envelope breakdown and chromosome condensation
    K R Yu
    Department of Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA
    Nat Cell Biol 2:609-15. 2000
    ..These results are likely to apply to vertebrate cells and suggest new strategies for destroying checkpoint-compromised cancer cells...
  61. ncbi Relationship of DNA double-strand breaks to synapsis in Drosophila
    Janet K Jang
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
    J Cell Sci 116:3069-77. 2003
    ..requirements for double-strand break formation during Drosophila meiosis, we used an antibody that recognizes a histone modification at double-strand break sites, phosphorylation of HIS2AV (gamma-HIS2AV)...
  62. pmc Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression
    Ruth McCaffrey
    MRC Laboratory of Molecular Biology, The Gurdon Institute and Department of Genetics, University of Cambridge, Cambridge CB2 1QR, United Kingdom
    Genetics 174:1273-85. 2006
    ..However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination...
  63. pmc Nucleosome organization in the Drosophila genome
    Travis N Mavrich
    Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nature 453:358-62. 2008
    ..Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from ..
  64. pmc Nuclear trapping shapes the terminal gradient in the Drosophila embryo
    Mathieu Coppey
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08540, USA
    Curr Biol 18:915-9. 2008
    ..Inside the syncytial embryo, nuclei play the role of traps that localize diffusible dpERK. We argue that the length scale of the terminal-patterning gradient is determined mainly by the intracellular module...
  65. doi Loss of RecQ5 leads to spontaneous mitotic defects and chromosomal aberrations in Drosophila melanogaster
    Minoru Nakayama
    Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge, Hirakata, Osaka 573 0101, Japan
    DNA Repair (Amst) 8:232-41. 2009
    ..Therefore, endogenous genomic damage may be produced in Drosophila development, and RecQ5 would be involved in the maintenance of genomic stability by suppressing the accumulation of DNA damage...
  66. pmc HorkaD, a chromosome instability-causing mutation in Drosophila, is a dominant-negative allele of Lodestar
    Tamas Szalontai
    Department of Biochemistry and Molecular Biology, University of Szeged, H 6726 Szeged, Hungary
    Genetics 181:367-77. 2009
    ..The Horka(D) mutation results in replacement of Ala777 with Thr, which we suggest causes chromosome instability by increasing the affinity of Lodestar for chromatin...
  67. pmc An assessment of histone-modification antibody quality
    Thea A Egelhofer
    Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, USA
    Nat Struct Mol Biol 18:91-3. 2011
    We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells...
  68. pmc Transcription initiation patterns indicate divergent strategies for gene regulation at the chromatin level
    Elizabeth A Rach
    Program in Computational Biology and Bioinformatics, Duke University, Durham, North Carolina, United States of America
    PLoS Genet 7:e1001274. 2011
    ..These differences extend to histone variants (H2A...
  69. pmc Evidence of activity-specific, radial organization of mitotic chromosomes in Drosophila
    Yuri G Strukov
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Biol 9:e1000574. 2011
    ..chromatin loci on mitotic chromosomes was found to be correlated with their functional activity as judged by core histone modifications...
  70. pmc Gebiss: an ImageJ plugin for the specification of ground truth and the performance evaluation of 3D segmentation algorithms
    Janos Kriston-Vizi
    Bioinformatics Institute, Agency for Science, Technology and Research A STAR, 138671, Singapore
    BMC Bioinformatics 12:232. 2011
    ..Scenarios include the quantification of 3D images with poor signal-to-noise ratios or the generation of so-called ground truth segmentations that are used to evaluate the accuracy of automated segmentation methods...
  71. pmc Genomic organization of H2Av containing nucleosomes in Drosophila heterochromatin
    Zhenhai Zhang
    Center for Comparative Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America
    PLoS ONE 6:e20511. 2011
    H2Av is a versatile histone variant that plays both positive and negative roles in transcription, DNA repair, and chromatin structure in Drosophila. H2Av, and its broader homolog H2A...
  72. pmc Cell cycle phase classification in 3D in vivo microscopy of Drosophila embryogenesis
    Tie Hua Du
    Imaging Informatics Division, Live Cell Imaging and Automation of Image Analysis Group Bioinformatics Institute, Agency for Science, Technology and Research, Singapore
    BMC Bioinformatics 12:S18. 2011
    ..Most existing studies have dealt with 2D images of cultured cells. Few, if any, studies have addressed the problem of cell cycle classification in 3D image stacks of intact tissues...
  73. doi Age- and oxidative stress-induced DNA damage in Drosophila intestinal stem cells as marked by Gamma-H2AX
    Joung Sun Park
    Department of Molecular Biology, Pusan National University, Busan 609 735, Republic of Korea
    Exp Gerontol 47:401-5. 2012
    ..midgut model for stem-cell biology, we demonstrated aging- and oxidative stress-related accumulation of γH2AvD foci, analogous to mammal γH2AX, in Drosophila intestinal stem cells (ISCs), and obtained evidence that the ..
  74. pmc Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1
    Friederike Althoff
    Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
    Mol Biol Cell 23:2275-91. 2012
    ..These findings further expand Mps1's significance for faithful mitotic chromosome segregation and emphasize the importance of its careful regulation...
  75. doi A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila development
    Kiriaki Kanakousaki
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
    Development 139:2751-62. 2012
    ..Supporting this view, knockdown of SUMO or its E1 and E2 enzymes robustly disrupts proliferating cells in the developing eye, without any detectable effects on the development or differentiation of neighboring post-mitotic cells...
  76. ncbi Conservation of intron position indicates separation of major and variant H2As is an early event in the evolution of eukaryotes
    A van Daal
    Department of Biology, Washington University, St Louis, Missouri 63130
    J Mol Evol 30:449-55. 1990
    ..of the variant and major cell cycle-regulated histone H2A proteins from several species indicates that the H2A variant proteins are evolutionarily separate and distinct from the major cell cycle-regulated histone H2A proteins...
  77. pmc Genetic characterization of ms (3) K81, a paternal effect gene of Drosophila melanogaster
    G K Yasuda
    Department of Zoology, University of Washington, Seattle 98195 1800, USA
    Genetics 140:219-29. 1995
    ..Based on the embryonic lethal phenotypes, we suggest that the K81+ gene encodes a sperm-specific product that is essential for the male pronucleus to participate in the first few embryonic nuclear divisions...
  78. ncbi The cyclin-dependent kinase inhibitor Roughex is involved in mitotic exit in Drosophila
    E Foley
    Institut fur Genetik, Universitat zu Koln, Weyertal 121, D 50931, Koln, Germany
    Curr Biol 11:151-60. 2001
    ..In contrast, the only mechanism known to be essential for CDK inactivation during mitosis in higher eukaryotes is cyclin destruction...
  79. pmc A complex degradation signal in Cyclin A required for G1 arrest, and a C-terminal region for mitosis
    H W Jacobs
    Department of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany
    EMBO J 20:2376-86. 2001
    ..However, we demonstrate that this failure of mitosis is not caused simply by DNA replication or damage checkpoints. Entry into mitosis requires a function of Cyclin A that does not depend on the presence of the N-terminal region...
  80. ncbi Toucan protein is essential for the assembly of syncytial mitotic spindles in Drosophila melanogaster
    A Debec
    Observatoire Oceanologique, Laboratoire de Biologie du Developpement, Université Pierre et Marie Curie CNRS, Villefranche sur Mer, France
    Genesis 31:167-75. 2001
    ..These results strongly suggest that Toucan represents a factor essential for the assembly and the function of the syncytial mitotic spindles...
  81. ncbi The centrosome is a dynamic structure that ejects PCM flares
    Timothy L Megraw
    Department of Biology, Indiana University, Bloomington, IN 47405, USA
    J Cell Sci 115:4707-18. 2002
    ..We propose that flare particles play a role in organizing the actin cytoskeleton during syncytial cleavage...
  82. ncbi The dual function of ovo/shavenbaby in germline and epidermis differentiation is conserved between Drosophila melanogaster and the olive fruit fly Bactrocera oleae
    Abderrahman Khila
    Laboratoire de Biotechnologie Végétale et Agro Alimentaire, Faculté des Sciences et Techniques de Fès Saïss, Université Sidi Mohammed Ben Abdellah, route de Imouzer, BP2202 Fès, Morocco
    Insect Biochem Mol Biol 33:691-9. 2003
    ..oleae svb embryonic expression precisely prefigures the pattern of larval trichomes, supporting the conclusion that regulatory changes in svb transcription underlie evolutionary diversification of trichome patterns seen among Dipterae...
  83. pmc Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytes
    Elena Rebollo
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    PLoS Biol 2:E8. 2004
    ..These observations are consistent with a model in which centrosomal and noncentrosomal microtubules contribute to the assembly and are required for the robustness of the cell division spindle in cells that contain centrosomes...
  84. pmc Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes
    Rabindra Tirouvanziam
    Herzenberg Laboratory, Department of Genetics, Stanford University School of Medicine, Beckman Center B007, Stanford, CA 94305 5318, USA
    Proc Natl Acad Sci U S A 101:2912-7. 2004
    ....
  85. ncbi Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster
    Ken ichi Kimura
    Laboratory of Biology, Iwamizawa Campus, Hokkaido University of Education, Iwamizawa, Hokkaido 068 8642, Japan
    Development 131:1597-606. 2004
    ..Thus, activation of the cAMP/PKA signaling pathway is required for transduction of the hormonal signal that induces wing epidermal cell death after eclosion...
  86. pmc The generation of cloned Drosophila melanogaster
    Andrew J Haigh
    Department of Biology, Dalhousie University, Halifax, Nova Scotia B4H 4J1, Canada
    Genetics 169:1165-7. 2005
    ....
  87. ncbi Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons
    Darren W Williams
    Department of Biology, University of Washington, Seattle, WA 98195, USA
    Development 132:3631-42. 2005
    ..Finally, we find that phagocytic blood cells not only engulf neuronal debris but also attack and sever intact branches that show signs of destabilization...
  88. ncbi Expression of COPI components during development of Drosophila melanogaster
    Nicole C Grieder
    Biozentrum der Universitat Basel, Abteilung Zellbiologie, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
    Gene Expr Patterns 6:11-21. 2005
    ....
  89. pmc All paired up with no place to go: pairing, synapsis, and DSB formation in a balancer heterozygote
    Wei J Gong
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 1:e67. 2005
    ....
  90. ncbi Somatic stem cell niche tropism in Wolbachia
    Horacio M Frydman
    Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey 08540, USA
    Nature 441:509-12. 2006
    ..This is, to our knowledge, the first report of an intracellular parasite displaying tropism for a stem cell niche...
  91. pmc Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila development
    Mary D Schneider
    Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G H7, Canada
    J Cell Biol 174:349-58. 2006
    ..This suggests that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development...
  92. pmc Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientation
    Cheng Yu Lee
    Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
    Genes Dev 20:3464-74. 2006
    ..We conclude that Aurora-A and Numb are novel inhibitors of neuroblast self-renewal and that spindle orientation regulates neuroblast self-renewal...
  93. pmc A Distinct type of heterochromatin within Drosophila melanogaster chromosome 4
    Karmella A Haynes
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    Genetics 175:1539-42. 2007
    ..distal arm chromosome 4 heterochromatin, distinguishing these two heterochromatin types...
  94. pmc Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster
    Ayako Sakaguchi
    Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA
    J Cell Biol 176:155-62. 2007
    PR-Set7 is a histone methyltransferase that specifically monomethylates histone H4 lysine 20 (K20) and is essential for cell proliferation. Our results show that in PR-Set7 mutants, the DNA damage checkpoint is activated...
  95. pmc Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogaster
    Anna Mia Johansson
    UCMP, Umea University, Umea, Sweden
    EMBO J 26:2307-16. 2007
    ..We propose a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome...
  96. pmc A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression
    Jianrong Lu
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:9381-6. 2007
    ..These findings suggest that potential chromatin regulation mediated by Domino and PcG-like factors plays an important role in controlling E2F activity and cell growth...
  97. pmc Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification
    David H Parma
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Dev Biol 308:507-19. 2007
    ..On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process...
  98. pmc RSF governs silent chromatin formation via histone H2Av replacement
    Kazuma Hanai
    Department of Developmental Genetics, National Institute of Genetics, Shizuoka ken, Japan
    PLoS Genet 4:e1000011. 2008
    ..In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin...
  99. pmc Identification of EMS-induced mutations in Drosophila melanogaster by whole-genome sequencing
    Justin P Blumenstiel
    Department of Physiology, Kansas University Medical Center, Kansas City, Kansas 66160, USA
    Genetics 182:25-32. 2009
    ..This new method of whole-genome sequencing represents great promise for mutant mapping in flies, potentially replacing conventional methods...
  100. doi The Drosophila hus1 gene is required for homologous recombination repair during meiosis
    Gabriella Peretz
    Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University, POB 653, Beer Sheva 84105, Israel
    Mech Dev 126:677-86. 2009
    ..Together, our results imply that hus1 is required for repair of DSBs during meiotic recombination...
  101. pmc The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
    Carla Klattenhoff
    University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 138:1137-49. 2009
    ..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle...

Research Grants9

  1. Cell Growth and Radiation Survival in Drosophila
    Tin Tin Su; Fiscal Year: 2007
    ..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
  2. Lipid droplets as sites for protein sequestration
    MICHAEL WELTE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  3. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  4. Mechanisms of Swallow protein function in Drosophila
    NANCY POKRYWKA; Fiscal Year: 2002
    ....
  5. Mini spindles and microtubule function in Drosophila
    NANCY POKRYWKA; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  6. Cell Cycle Regulation by Checkpoints in Drosophila
    Tin Tin Su; Fiscal Year: 2006
    ..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
  7. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  8. Lipid Droplets: Metabolic Consequences of Stored Neutral Lipids
    Dawn Brasaemle; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  9. An in vivo Screen for Radiation Sensitizers
    Tin Tin Su; Fiscal Year: 2008
    ..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. [unreadable] [unreadable] [unreadable]..