Genomes and Genes
Gene Symbol: His2Av
Description: Histone H2A variant
Alias: 5499, CG5499, Dmel\CG5499, H2A, H2A.F/Z, H2A.V, H2A.X, H2A.Z, H2AV, H2AV_DROM, H2AX, H2AZ, H2Av, H2AvD, H2a.V, H2av, H2avD, HIS, HIS2AV, HIS2AVD, His, His2, His2A, His2AV, His2AvD, HisH2Av, Hist, Hist2av, gamma-H2Av, gamma-HIS2AV, gamma-His2Av, gammaH2Av, h2AvD, his, his2Av, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602, CG5499-PA, CG5499-PB, H2A variant, His2Av-PA, His2Av-PB, Histone-2A, Histone-2av, histone, histone 2A variant, histone H2A variant, histone H2Av, histone variant H2Av, histone2A, histone2Av, histone2AvD
Species: fruit fly
Publications102 found, 100 shown here
- Regions of variant histone His2AvD required for Drosophila developmentM J Clarkson
The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory
Nature 399:694-7. 1999..domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes...
- Greatwall kinase: a nuclear protein required for proper chromosome condensation and mitotic progression in DrosophilaJiangtao Yu
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853 2703, USA
J Cell Biol 164:487-92. 2004..Chromosomes are highly undercondensed, particularly in the euchromatin, but nevertheless contain phosphorylated histone H3, condensin, and topoisomerase II...
- Genome-wide profiling of salt fractions maps physical properties of chromatinSteven Henikoff
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
Genome Res 19:460-9. 2009..3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3...
- Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphaseMelina Schuh
Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
Curr Biol 17:237-43. 2007..A candidate for an epigenetic mark is the centromere-specific histone H3 variant CENP-A that replaces H3 in alternating blocks of chromatin exclusively in active centromeres...
- Acetylation by Tip60 is required for selective histone variant exchange at DNA lesionsThomas Kusch
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
Science 306:2084-7. 2004Phosphorylation of the human histone variant H2A.X and H2Av, its homolog in Drosophila melanogaster, occurs rapidly at sites of DNA double-strand breaks...
- A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytesIrena Ivanovska
Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
Genes Dev 19:2571-82. 2005..We describe a mutation in a recently identified Histone H2A kinase, nhk-1, in Drosophila that leads to female sterility due to defects in the formation of the meiotic ..
- PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigenS Kotadia
Department of Pharmacology, The Cecil and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9051, USA
Oncogene 27:6334-46. 2008..These analyses demonstrate the validity and utility of Drosophila as a model for viral oncoprotein function in vivo...
- Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage responseCarla Klattenhoff
Program in Molecular Medicine and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
Dev Cell 12:45-55. 2007..Furthermore, rasiRNA pathway mutations lead to germline-specific accumulation of gamma-H2Av foci characteristic of DNA damage...
- Mitosis in primary cultures of Drosophila melanogaster larval neuroblastsMatthew S Savoian
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, P O Box 509, Albany, New York 12201 0509, USA
J Cell Sci 115:3061-72. 2002..5 minutes. Analysis of neuroblasts containing various GFP-tagged proteins (e.g. histone, fizzy, fizzy-related and alpha-tubulin) reveals that attaching kinetochores exhibit sudden, rapid pole-directed ..
- Drosophila PCH2 is required for a pachytene checkpoint that monitors double-strand-break-independent events leading to meiotic crossover formationEric F Joyce
Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854 8020, USA
Genetics 181:39-51. 2009..Interestingly, PCH2-dependent delays in prophase may allow additional crossovers to form...
- Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivoDmitry V Fyodorov
Section of Molecular Biology, University of California at San Diego, La Jolla, CA 92093, USA
Genes Dev 18:170-83. 2004..These findings collectively indicate that ACF/CHRAC functions in the assembly of periodic nucleosome arrays that contribute to the repression of genetic activity in the eukaryotic nucleus...
- Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulationAnne Royou
Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
J Cell Biol 183:63-75. 2008..Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms...
- The cohesion protein ORD is required for homologue bias during meiotic recombinationHayley A Webber
Dept of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, NH 03755, USA
J Cell Biol 164:819-29. 2004..We conclude that ORD activity suppresses sister chromatid exchange and stimulates inter-homologue crossovers, thereby promoting homologue bias during meiotic recombination in Drosophila...
- Influence of cyclin type and dose on mitotic entry and progression in the early Drosophila embryoMark L McCleland
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
J Cell Biol 184:639-46. 2009....
- Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in DrosophilaJun Yuan Ji
Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
Development 131:401-11. 2004....
- The degradation of two mitotic cyclins contributes to the timing of cytokinesisArnaud Echard
Department of Biochemistry and Biophysics, University of California, San Francisco, Genentech Hall, 600 16th Street, San Francisco, CA 94143, USA
Curr Biol 13:373-83. 2003..We examined the interface between mitotic cyclin destruction and the timing of cytokinesis...
- DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosisJames P Madigan
Wadsworth Center, New York State Department of Health, State University of New York, Albany, NY 12201 2002, USA
Nucleic Acids Res 30:3698-705. 2002..We demonstrate the DSB-induced phosphorylation of histone variant H2Av in Drosophila melanogaster. H2Av is a member of the H2AZ family of histone variants...
- The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosisD H Parry
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
Curr Biol 11:671-83. 2001..We tested a recent proposal that the destruction of the different cyclins guides progress through mitosis...
- A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behaviorM Clarkson
Department of Biochemistry, University of Adelaide, SA, Australia
DNA Cell Biol 18:457-62. 1999..melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria...
- Onset of the DNA replication checkpoint in the early Drosophila embryoJustin Crest
Department of Biology, University of Washington, Seattle, Washington 98195 1800, USA
Genetics 175:567-84. 2007..Our results suggest an antagonistic interaction between DNA replication checkpoint activation and Cdk1-CycB activity during the transition from preblastoderm to blastoderm cycles...
- Regulation of mitosis in response to damaged or incompletely replicated DNA require different levels of Grapes (Drosophila Chk1)Amanda Purdy
MCD Biology, University of Colorado, Boulder, CO 80309 0347, USA
J Cell Sci 118:3305-15. 2005..Finally, our results demonstrate that Drosophila provides a unique opportunity to study the regulation of the entry into, and progress through, mitosis by DNA structure checkpoints in metazoa...
- Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleusSunil Jayaramaiah Raja
Philipps Universitat Marburg, Fachbereich Biologie, Entwicklungsbiologie, 35043 Marburg, Germany
Mol Cell Biol 25:6165-77. 2005..Mst77F encodes a linker histone-like protein showing significant similarity to mammalian HILS1 protein...
- RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycleMark L McCleland
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 2200, USA
Curr Biol 18:245-54. 2008..However, we still know very little of how individual cell-cycle events are coupled to this oscillator and how the timing of each event is controlled...
- The role of histone H2Av variant replacement and histone H4 acetylation in the establishment of Drosophila heterochromatinJyothishmathi Swaminathan
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA
Genes Dev 19:65-76. 2005..Here we show that the histone H2A variant of Drosophila melanogaster, H2Av, localizes to the centromeric heterochromatin, and it is recruited to an ..
- Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesisJason Stumpff
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Curr Biol 14:2143-8. 2004..These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase...
- Temporal analysis of meiotic DNA double-strand break formation and repair in Drosophila femalesS Mehrotra
Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, New Jersey, United States of America
PLoS Genet 2:e200. 2006Using an antibody against the phosphorylated form of His2Av (gamma-His2Av), we have described the time course for the series of events leading from the formation of a double-strand break (DSB) to a crossover in Drosophila female meiotic ..
- Nucleosome organization in the Drosophila genomeTravis N Mavrich
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
Nature 453:358-62. 2008..Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from ..
- Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progressionRuth McCaffrey
MRC Laboratory of Molecular Biology, The Gurdon Institute and Department of Genetics, University of Cambridge, Cambridge CB2 1QR, United Kingdom
Genetics 174:1273-85. 2006..However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination...
- Histone H2A.Z is widely but nonrandomly distributed in chromosomes of Drosophila melanogasterT J Leach
Wadsworth Center, New York State Department of Health and Department of Biomedical Sciences, State University of New York, Albany, New York 12201 2002, USA
J Biol Chem 275:23267-72. 2000..H2A.F/Z is a highly conserved family of H2A variants. H2Av, the H2A...
- Relationship of DNA double-strand breaks to synapsis in DrosophilaJanet K Jang
Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, 190 Frelinghuysen Rd, Piscataway, New Jersey 08854 8020, USA
J Cell Sci 116:3069-77. 2003..requirements for double-strand break formation during Drosophila meiosis, we used an antibody that recognizes a histone modification at double-strand break sites, phosphorylation of HIS2AV (gamma-HIS2AV)...
- A histone variant, H2AvD, is essential in Drosophila melanogasterA van Daal
Department of Biology, Washington University, St Louis, Missouri 63130
Mol Biol Cell 3:593-602. 1992H2AvD, a Drosophila melanogaster histone variant of the H2A.Z class, is encoded by a single copy gene in the 97CD region of the polytene chromosomes...
- The Grapes checkpoint coordinates nuclear envelope breakdown and chromosome condensationK R Yu
Department of Biology, University of California at Santa Cruz, Santa Cruz, California 95064, USA
Nat Cell Biol 2:609-15. 2000..These results are likely to apply to vertebrate cells and suggest new strategies for destroying checkpoint-compromised cancer cells...
- HorkaD, a chromosome instability-causing mutation in Drosophila, is a dominant-negative allele of LodestarTamas Szalontai
Department of Biochemistry and Molecular Biology, University of Szeged, H 6726 Szeged, Hungary
Genetics 181:367-77. 2009..The Horka(D) mutation results in replacement of Ala777 with Thr, which we suggest causes chromosome instability by increasing the affinity of Lodestar for chromatin...
- Gebiss: an ImageJ plugin for the specification of ground truth and the performance evaluation of 3D segmentation algorithmsJanos Kriston-Vizi
Bioinformatics Institute, Agency for Science, Technology and Research A STAR, 138671, Singapore
BMC Bioinformatics 12:232. 2011..Scenarios include the quantification of 3D images with poor signal-to-noise ratios or the generation of so-called ground truth segmentations that are used to evaluate the accuracy of automated segmentation methods...
- Genomic organization of H2Av containing nucleosomes in Drosophila heterochromatinZhenhai Zhang
Center for Comparative Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America
PLoS ONE 6:e20511. 2011H2Av is a versatile histone variant that plays both positive and negative roles in transcription, DNA repair, and chromatin structure in Drosophila. H2Av, and its broader homolog H2A...
- Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repairEric F Joyce
Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ 08854, USA
J Cell Biol 195:359-67. 2011..Both kinases phosphorylate H2AV (γ-H2AV), and, using this as a reporter for ATM/ATR activity, we found that the DSB repair response is ..
- Cell cycle phase classification in 3D in vivo microscopy of Drosophila embryogenesisTie Hua Du
Imaging Informatics Division, Live Cell Imaging and Automation of Image Analysis Group Bioinformatics Institute, Agency for Science, Technology and Research, Singapore
BMC Bioinformatics 12:S18. 2011..Most existing studies have dealt with 2D images of cultured cells. Few, if any, studies have addressed the problem of cell cycle classification in 3D image stacks of intact tissues...
- Loss of RecQ5 leads to spontaneous mitotic defects and chromosomal aberrations in Drosophila melanogasterMinoru Nakayama
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, 45 1 Nagaotoge, Hirakata, Osaka 573 0101, Japan
DNA Repair (Amst) 8:232-41. 2009..Therefore, endogenous genomic damage may be produced in Drosophila development, and RecQ5 would be involved in the maintenance of genomic stability by suppressing the accumulation of DNA damage...
- Age- and oxidative stress-induced DNA damage in Drosophila intestinal stem cells as marked by Gamma-H2AXJoung Sun Park
Department of Molecular Biology, Pusan National University, Busan 609 735, Republic of Korea
Exp Gerontol 47:401-5. 2012..midgut model for stem-cell biology, we demonstrated aging- and oxidative stress-related accumulation of γH2AvD foci, analogous to mammal γH2AX, in Drosophila intestinal stem cells (ISCs), and obtained evidence that the ..
- Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1Friederike Althoff
Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
Mol Biol Cell 23:2275-91. 2012..These findings further expand Mps1's significance for faithful mitotic chromosome segregation and emphasize the importance of its careful regulation...
- A differential requirement for SUMOylation in proliferating and non-proliferating cells during Drosophila developmentKiriaki Kanakousaki
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA
Development 139:2751-62. 2012..Supporting this view, knockdown of SUMO or its E1 and E2 enzymes robustly disrupts proliferating cells in the developing eye, without any detectable effects on the development or differentiation of neighboring post-mitotic cells...
- Evidence of activity-specific, radial organization of mitotic chromosomes in DrosophilaYuri G Strukov
Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
PLoS Biol 9:e1000574. 2011..chromatin loci on mitotic chromosomes was found to be correlated with their functional activity as judged by core histone modifications...
- Nuclear trapping shapes the terminal gradient in the Drosophila embryoMathieu Coppey
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08540, USA
Curr Biol 18:915-9. 2008..Inside the syncytial embryo, nuclei play the role of traps that localize diffusible dpERK. We argue that the length scale of the terminal-patterning gradient is determined mainly by the intracellular module...
- Transcription initiation patterns indicate divergent strategies for gene regulation at the chromatin levelElizabeth A Rach
Program in Computational Biology and Bioinformatics, Duke University, Durham, North Carolina, United States of America
PLoS Genet 7:e1001274. 2011..These differences extend to histone variants (H2A...
- An assessment of histone-modification antibody qualityThea A Egelhofer
Department of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, California, USA
Nat Struct Mol Biol 18:91-3. 2011We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells...
- Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletionHaifeng Wang
Department of Molecular and Cell Biology, One Shields Avenue, University of California Davis, Davis, California 95616, USA
Cytoskeleton (Hoboken) 67:715-28. 2010....
- The centrosome is a dynamic structure that ejects PCM flaresTimothy L Megraw
Department of Biology, Indiana University, Bloomington, IN 47405, USA
J Cell Sci 115:4707-18. 2002..We propose that flare particles play a role in organizing the actin cytoskeleton during syncytial cleavage...
- The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clustersCarla Klattenhoff
University of Massachusetts Medical School, Worcester, MA 01605, USA
Cell 138:1137-49. 2009..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle...
- A Distinct type of heterochromatin within Drosophila melanogaster chromosome 4Karmella A Haynes
Department of Biology, Washington University, St Louis, Missouri 63130, USA
Genetics 175:1539-42. 2007..distal arm chromosome 4 heterochromatin, distinguishing these two heterochromatin types...
- Drosophila Aurora-A kinase inhibits neuroblast self-renewal by regulating aPKC/Numb cortical polarity and spindle orientationCheng Yu Lee
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA
Genes Dev 20:3464-74. 2006..We conclude that Aurora-A and Numb are novel inhibitors of neuroblast self-renewal and that spindle orientation regulates neuroblast self-renewal...
- Transcriptional and developmental functions of the H3.3 histone variant in DrosophilaAkiko Sakai
Department of BCMP, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
Curr Biol 19:1816-20. 2009..In most animals, replacement uses the conserved H3.3 histone variant, but the functions of this variant have not been defined. Using mutations for the two H3...
- Identification of EMS-induced mutations in Drosophila melanogaster by whole-genome sequencingJustin P Blumenstiel
Department of Physiology, Kansas University Medical Center, Kansas City, Kansas 66160, USA
Genetics 182:25-32. 2009..This new method of whole-genome sequencing represents great promise for mutant mapping in flies, potentially replacing conventional methods...
- Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loadingHeather K MacAlpine
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Genome Res 20:201-11. 2010..ORC-associated sequences are enriched for the histone variant, H3.3, often at transcription start sites, and depleted for bulk nucleosomes...
- Loss of the histone variant H2A.Z restores capping to checkpoint-defective telomeres in DrosophilaYikang S Rong
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genetics 180:1869-75. 2008The conserved histone variant H2A.Z fulfills many functions by being an integral part of the nucleosomes placed at specific regions of the genome...
- Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogasterAnna Mia Johansson
UCMP, Umea University, Umea, Sweden
EMBO J 26:2307-16. 2007..We propose a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome...
- The cyclin-dependent kinase inhibitor Dacapo promotes replication licensing during Drosophila endocyclesAmy Hong
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
EMBO J 26:2071-82. 2007..Finally, genetic interaction studies suggest that dap functions to promote replication licensing in a subset of Drosophila mitotic cycles...
- A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repressionJianrong Lu
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:9381-6. 2007..These findings suggest that potential chromatin regulation mediated by Domino and PcG-like factors plays an important role in controlling E2F activity and cell growth...
- Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specificationDavid H Parma
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Dev Biol 308:507-19. 2007..On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process...
- The Drosophila hus1 gene is required for homologous recombination repair during meiosisGabriella Peretz
Department of Life Sciences and the National Institute for Biotechnology in the Negev, Ben Gurion University, POB 653, Beer Sheva 84105, Israel
Mech Dev 126:677-86. 2009..Together, our results imply that hus1 is required for repair of DSBs during meiotic recombination...
- RSF governs silent chromatin formation via histone H2Av replacementKazuma Hanai
Department of Developmental Genetics, National Institute of Genetics, Shizuoka ken, Japan
PLoS Genet 4:e1000011. 2008..In dRsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin...
- Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogasterAyako Sakaguchi
Waksman Institute, Department of Molecular Biology and Biochemistry, Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, USA
J Cell Biol 176:155-62. 2007PR-Set7 is a histone methyltransferase that specifically monomethylates histone H4 lysine 20 (K20) and is essential for cell proliferation. Our results show that in PR-Set7 mutants, the DNA damage checkpoint is activated...
- All paired up with no place to go: pairing, synapsis, and DSB formation in a balancer heterozygoteWei J Gong
Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
PLoS Genet 1:e67. 2005....
- The cyclin-dependent kinase inhibitor Roughex is involved in mitotic exit in DrosophilaE Foley
Institut fur Genetik, Universitat zu Koln, Weyertal 121, D 50931, Koln, Germany
Curr Biol 11:151-60. 2001..In contrast, the only mechanism known to be essential for CDK inactivation during mitosis in higher eukaryotes is cyclin destruction...
- The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1Matthias G Fischer
BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
Curr Biol 14:2019-24. 2004..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
- The generation of cloned Drosophila melanogasterAndrew J Haigh
Department of Biology, Dalhousie University, Halifax, Nova Scotia B4H 4J1, Canada
Genetics 169:1165-7. 2005....
- Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogasterKen ichi Kimura
Laboratory of Biology, Iwamizawa Campus, Hokkaido University of Education, Iwamizawa, Hokkaido 068 8642, Japan
Development 131:1597-606. 2004..Thus, activation of the cAMP/PKA signaling pathway is required for transduction of the hormonal signal that induces wing epidermal cell death after eclosion...
- Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytesRabindra Tirouvanziam
Herzenberg Laboratory, Department of Genetics, Stanford University School of Medicine, Beckman Center B007, Stanford, CA 94305 5318, USA
Proc Natl Acad Sci U S A 101:2912-7. 2004....
- Contribution of noncentrosomal microtubules to spindle assembly in Drosophila spermatocytesElena Rebollo
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany
PLoS Biol 2:E8. 2004..These observations are consistent with a model in which centrosomal and noncentrosomal microtubules contribute to the assembly and are required for the robustness of the cell division spindle in cells that contain centrosomes...
- A complex degradation signal in Cyclin A required for G1 arrest, and a C-terminal region for mitosisH W Jacobs
Department of Genetics, University of Bayreuth, D 95440 Bayreuth, Germany
EMBO J 20:2376-86. 2001..However, we demonstrate that this failure of mitosis is not caused simply by DNA replication or damage checkpoints. Entry into mitosis requires a function of Cyclin A that does not depend on the presence of the N-terminal region...
- Toucan protein is essential for the assembly of syncytial mitotic spindles in Drosophila melanogasterA Debec
Observatoire Oceanologique, Laboratoire de Biologie du Developpement, Université Pierre et Marie Curie CNRS, Villefranche sur Mer, France
Genesis 31:167-75. 2001..These results strongly suggest that Toucan represents a factor essential for the assembly and the function of the syncytial mitotic spindles...
- The dual function of ovo/shavenbaby in germline and epidermis differentiation is conserved between Drosophila melanogaster and the olive fruit fly Bactrocera oleaeAbderrahman Khila
Laboratoire de Biotechnologie Végétale et Agro Alimentaire, Faculté des Sciences et Techniques de Fès Saïss, Université Sidi Mohammed Ben Abdellah, route de Imouzer, BP2202 Fès, Morocco
Insect Biochem Mol Biol 33:691-9. 2003..oleae svb embryonic expression precisely prefigures the pattern of larval trichomes, supporting the conclusion that regulatory changes in svb transcription underlie evolutionary diversification of trichome patterns seen among Dipterae...
- The Drosophila EAST protein associates with a nuclear remnant during mitosis and constrains chromosome mobilityMartin Wasser
Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609
J Cell Sci 116:1733-43. 2003..We propose that EAST constitutes a component of a nucleoskeleton that helps to constrain the mobility of chromosomes in interphase, mitosis and meiosis...
- Epithelial re-organization and dynamics of progression through mitosis in Drosophila separase complex mutantsRahul Pandey
BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
J Cell Sci 118:733-42. 2005....
- Conservation of intron position indicates separation of major and variant H2As is an early event in the evolution of eukaryotesA van Daal
Department of Biology, Washington University, St Louis, Missouri 63130
J Mol Evol 30:449-55. 1990..of the variant and major cell cycle-regulated histone H2A proteins from several species indicates that the H2A variant proteins are evolutionarily separate and distinct from the major cell cycle-regulated histone H2A proteins...
- Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neuronsDarren W Williams
Department of Biology, University of Washington, Seattle, WA 98195, USA
Development 132:3631-42. 2005..Finally, we find that phagocytic blood cells not only engulf neuronal debris but also attack and sever intact branches that show signs of destabilization...
- Gawky is a component of cytoplasmic mRNA processing bodies required for early Drosophila developmentMary D Schneider
Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G H7, Canada
J Cell Biol 174:349-58. 2006..This suggests that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development...
- The EAST protein of drosophila controls an expandable nuclear endoskeletonM Wasser
Institute of Molecular and Cell Biology, National University of Singapore
Nat Cell Biol 2:268-75. 2000..We propose that EAST mediates the assembly of an expandable nuclear endoskeleton which, through alterations of its volume, can modulate the spatial arrangement of chromosomes...
- Somatic stem cell niche tropism in WolbachiaHoracio M Frydman
Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey 08540, USA
Nature 441:509-12. 2006..This is, to our knowledge, the first report of an intracellular parasite displaying tropism for a stem cell niche...
- Expression of COPI components during development of Drosophila melanogasterNicole C Grieder
Biozentrum der Universitat Basel, Abteilung Zellbiologie, Klingelbergstrasse 50 70, CH 4056 Basel, Switzerland
Gene Expr Patterns 6:11-21. 2005....
- Genetic characterization of ms (3) K81, a paternal effect gene of Drosophila melanogasterG K Yasuda
Department of Zoology, University of Washington, Seattle 98195 1800, USA
Genetics 140:219-29. 1995..Based on the embryonic lethal phenotypes, we suggest that the K81+ gene encodes a sperm-specific product that is essential for the male pronucleus to participate in the first few embryonic nuclear divisions...
- Ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryosNadia Trieselmann
Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Ontario M5S 1A8, Canada
Curr Biol 12:1124-9. 2002..In contrast, nuclear proteins redistribute throughout the embryo upon nuclear envelope breakdown (NEB). Thus, in vivo RanGTP has the correct spatial localization within the cell to modulate spindle assembly...
- The importin-beta P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelopeGyula Timinszky
The University of Szeged, Faculty of Medicine, Department of Biology, Somogyi B u 4, H 6720 Szeged, Hungary
J Cell Sci 115:1675-87. 2002..However, it interferes with nuclear envelope formation during mitosis-to-interphase transition, revealing a novel function of importin-beta...
- Hypoxia and nitric oxide induce a rapid, reversible cell cycle arrest of the Drosophila syncytial divisionsP J DiGregorio
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143, USA
J Biol Chem 276:1930-7. 2001..We examined this rapid arrest in real time using a fusion of green fluorescent protein and histone 2A. In addition to an interphase arrest, mitosis was specifically blocked in metaphase, much like a checkpoint ..
- DNA damage leads to a Cyclin A-dependent delay in metaphase-anaphase transition in the Drosophila gastrulaT T Su
MCD Biology, University of Colorado, Boulder, CO 80309, USA
Curr Biol 11:8-17. 2001..The resulting polyploid nuclei are eliminated...
- Genetic analysis of chromosomal region 97D2-9 of Drosophila melanogasterW R Knibb
Department of Biochemistry, University of Adelaide, Australia
Mol Gen Genet 239:109-14. 1993..In addition to rough, one other locus within this region, 1(3)97De, was shown to be required for formation of the normal pattern of photoreceptor cells in the compound eye...
- Developmental biology: holding pattern for histonesDawn L Brasaemle
Department of Nutritional Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
Curr Biol 16:R918-20. 2006..New research on lipid droplets in Drosophila embryos has led to the surprising conclusion that these poorly understood organelles have a novel role as a regulated storage depot of maternally supplied proteins, particularly histones...
- Pictures in cell biology. A functional marker for Drosophila chromosomes in vivoR Saint
Centre for the Molecular Genetics of Development and Dept of Molecular Biosciences, University of Adelaide, SA 5005, Adelaide, Australia
Trends Cell Biol 10:553. 2000
- Completion of mitosis requires neither fzr/rap nor fzr2, a male germline-specific Drosophila Cdh1 homologHenning Jacobs
Department of Genetics, University of Bayreuth, 95440, Bayreuth, Germany
Curr Biol 12:1435-41. 2002..However, fzr is an essential gene corresponding to the rap locus, and FZR, which accumulates predominantly in the cytoplasm, is clearly required during G1...
- Drosophila APC2 and APC1 have overlapping roles in the larval brain despite their distinct intracellular localizationsKathryn Akong
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599 3280, USA
Dev Biol 250:71-90. 2002..Our experiments suggest that this does not result from misregulation of Wg signaling, and thus may involve the cytoskeletal or adhesive roles of APC proteins...
- The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesisJuehua Yu
Institute of Developmental Biology and Molecular Medicine, Morgan Tan International Center for Life Sciences, School of Life Science, Fudan University, Shanghai, China
Dev Biol 289:336-45. 2006..These results indicate that maternal MSP-300 plays an important role in actin-dependent nuclear anchorage during cytoplasmic transport...
- Synthesis of the sulfate donor PAPS in either the Drosophila germline or somatic follicle cells can support embryonic dorsal-ventral axis formationXianjun Zhu
Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
Development 134:1465-9. 2007..This finding indicates the existence of a conduit for the movement of PAPS between the germline and the follicle cells, which highlights a previously unappreciated mechanism of soma/germline cooperation affecting pattern formation...
- Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogasterDaniel Pérez-Mongiovi
Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
Development 132:4509-20. 2005..Our results suggest that BubR1 is required for normal synchrony and progression of syncytial nuclei through mitosis and to maintain the mitotic arrest of the polar body chromosomes after completion of meiosis...
- grp (chk1) replication-checkpoint mutations and DNA damage trigger a Chk2-dependent block at the Drosophila midblastula transitionSaeko Takada
Program in Molecular Medicine, Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
Development 134:1737-44. 2007..We conclude that the DNA-replication checkpoint maintains genome integrity during the cleavage divisions, and that checkpoint mutations lead to DNA damage that induces a novel Chk2-dependent block at the MBT...
- Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homologSebastian Heeger
Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
Genes Dev 19:2041-53. 2005....
- Genetic interactions between Cdk1-CyclinB and the Separase complex in DrosophilaJun Yuan Ji
Department of Biology, University of Washington, Seattle, WA 98195 1800, USA
Development 132:1875-84. 2005....
- One- and two-photon photoactivation of a paGFP-fusion protein in live Drosophila embryosJanine N Post
Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
FEBS Lett 579:325-30. 2005We constructed a photoactivatable Drosophila histone 2 A variant green fluorescent fusion protein (H2AvD-paGFP) for tracking chromatin loci in living Drosophila embryos...
- The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motilityMijung Kwon
University of California Davis, Davis, California 95616, USA
Mol Biol Cell 15:219-33. 2004..We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility...
- Activation of facultatively silenced Drosophila loci associates with increased acetylation of histone H2AvDMasahiko Tanabe
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo ku, Tokyo, Japan
Genes Cells 13:1279-88. 2008H2A.Z is an evolutionarily highly conserved non-allelic variant of histone H2A. H2A.Z and its homologues have been shown to involve in both chromatin silencing and activation. Although much of our knowledge of H2A...
- Control of cleavage cycles in Drosophila embryos by frühstartJörg Grosshans
ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany
Dev Cell 5:285-94. 2003..Furthermore, the expression of frs is delayed in haploid embryos and requires activity of the maternal checkpoint gene grapes. We propose that zygotic frs expression is involved in linking the N/C and the pause of cleavage cycle...
- Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryosD J Sharp
Section of Molecular and Cellular Biology, University of California Davis, 1 Shields Avenue, Davis, California 95616, USA
Nat Cell Biol 2:922-30. 2000..Thus, dynein is essential for poleward chromosome motility throughout mitosis in Drosophila embryos...
- Postsynaptic mad signaling at the Drosophila neuromuscular junctionVeronica Dudu
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Curr Biol 16:625-35. 2006..There is evidence that a retrograde, presynaptic transforming growth factor-beta (TGF-beta) signaling event regulates synapse development and function in Drosophila...
- Cell Growth and Radiation Survival in DrosophilaTin Tin Su; Fiscal Year: 2007..Understanding radiation survival in a multicellular context could help identify potential therapies for when we do not want cells to survive irradiation, such as in the treatment of cancer. ..
- ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSISSteven Henikoff; Fiscal Year: 2001..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
- Mechanisms of Swallow protein function in DrosophilaNANCY POKRYWKA; Fiscal Year: 2002....
- Mini spindles and microtubule function in DrosophilaNANCY POKRYWKA; Fiscal Year: 2006..unreadable] [unreadable]..
- Cell Cycle Regulation by Checkpoints in DrosophilaTin Tin Su; Fiscal Year: 2006..Aim 4) We will screen through existing mutagensensitive Drosophila mutants for their ability to regulate mitosis, S phase and cell death after irradiation, in order to uncover novel genes needed for proper response to DNA damage. ..
- Maintenance of blocks-based tools for functional genomicSteven Henikoff; Fiscal Year: 2006..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
- Lipid Droplets: Metabolic Consequences of Stored Neutral LipidsDawn Brasaemle; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- An in vivo Screen for Radiation SensitizersTin Tin Su; Fiscal Year: 2008..Successful completion of this project would establish a novel, rapid and effective primary screen in vivo for radiosensitizers with potential therapeutic value. [unreadable] [unreadable] [unreadable]..