Genomes and Genes
Gene Symbol: gammaTub37C
Description: gamma-Tubulin at 37C
Alias: CG17566, D.m.GAMMA-37C, Dmel\CG17566, GTU88*, PL10, Rb1011, TW1, Tub3B, fs(2)TW1, fs(2)TWI, fsTW1, g-Tubulin, gamma Tub37CD, gamma tubulin, gamma-Tub, gamma-Tub37C, gamma-tub, gamma-tub37C, gamma-tub37CD, gamma-tubulin, gammaTUB, gammaTUB37C, gammaTub, gammaTub2, gammaTub37CD, gammaTub37c, gamma[mat], gammatub, CG17566 gene product from transcript CG17566-RB, 37CD gamma-tubulin, CG17566-PA, CG17566-PB, female sterile(2)Ted wright 1, gama-tubulin, gamma Tubulin2, gamma tubulin, gamma tubulin at 37C, gamma-Tubulin, gamma-tubulin (37C), gamma-tubulin-2, gamma-tubulin37C, gammaTub37C-PA, gammaTub37C-PB, gammatubulin, maternal gamma-tubulin, tubulin
Species: fruit fly
Publications108 found, 100 shown here
- Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosomeY Zheng
Department of Molecular Genetics, Ohio State University, Columbus 43210 1292
Cell 65:817-23. 1991The mipA gene of A. nidulans encodes a newly discovered member of the tubulin superfamily of proteins, gamma-tubulin. In A...
- Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubulesK Oegema
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Cell Biol 144:721-33. 1999gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1- 12)...
- Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindleMark A Hallen
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Biophys J 95:3048-58. 2008..Here we use it to analyze gamma-tubulin binding to the mitotic spindle and centrosomes to determine the role of gamma-tubulin in microtubule nucleation ..
- Maternally expressed gamma Tub37CD in Drosophila is differentially required for female meiosis and embryonic mitosisP G Wilson
Laboratory of Molecular Biology, University of Wisconsin, Madison 53705, USA
Dev Biol 199:273-90. 1998We report functional analysis of gamma Tub37CD, a maternally synthesized gamma-tubulin that is highly expressed during oogenesis and utilized at centrosomes in precellular embryos...
- Drosophila Lissencephaly-1 functions with Bic-D and dynein in oocyte determination and nuclear positioningA Swan
Department of Biology, McGill University, 1205 Dr Penfield, Montreal, Quebec H3A 1B1, Canada
Nat Cell Biol 1:444-9. 1999....
- PAR-1 is required for the maintenance of oocyte fate in DrosophilaJ R Huynh
The Wellcome CRC Institute, University of Cambridge, Tennis Court Rd, Cambridge CB2 1QR, UK
Development 128:1201-9. 2001..Finally, we show that PAR-1 localises on the fusome, and provides a link between the asymmetry of the fusome and the selection of the oocyte...
- Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centersM do Carmo Avides
Cancer Research Campaign Cell Cycle Genetics Laboratories, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK
Science 283:1733-5. 1999..This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating gamma-tubulin ring complexes that organize the mitotic centrosome.
- The centrosomin protein is required for centrosome assembly and function during cleavage in DrosophilaT L Megraw
Department of Biology, Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA
Development 126:2829-39. 1999..poles in the mutants contain little or no detectable amounts of the centrosomal proteins CP60, CP190 and (gamma)-tubulin and late stage embryos often do not have astral microtubules at their spindle poles...
- Gamma-tubulin complexes: size does matterR Jeng
Dept of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
Trends Cell Biol 9:339-42. 1999gamma-Tubulin is a conserved component of all microtubule-organizing centres and is required for these organelles to nucleate microtubule polymerization. However, the mechanism of nucleation is not known...
- Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviourM J Lee
Department of Genetics, Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
Nat Cell Biol 3:643-9. 2001..We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome...
- Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomesDong Zhang
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
J Cell Biol 177:231-42. 2007..attached microtubule plus ends (Pacman) while being reeled in to spindle poles by the continual poleward flow of tubulin subunits driven by minus-end depolymerization (flux)...
- Three-dimensional structural characterization of centrosomes from early Drosophila embryosM Moritz
Department of Biochemistry and Biophysics, University of California at San Francisco 94143 0448, USA
J Cell Biol 130:1149-59. 1995....
- Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindleI Lajoie-Mazenc
Laboratoire de Pharmacologie et de Toxicologie Fondamentales CNRS, Toulouse, France
J Cell Sci 107:2825-37. 1994It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation...
- Differential expression of two gamma-tubulin isoforms during gametogenesis and development in DrosophilaP G Wilson
Department of Developmental Biology, Stanford University School of Medicine, California 94305, USA
Dev Biol 184:207-21. 1997..1991). We now report identification of a second gamma-tubulin gene, gamma Tub37CD. Immunoblot analysis and immunolocalization show that gamma Tub37CD and gamma Tub23C are differentially ..
- Essential role for gamma-tubulin in the acentriolar female meiotic spindle of DrosophilaG Tavosanis
European Molecular Biology Laboratory, Heidelberg, Germany
EMBO J 16:1809-19. 1997..In Drosophila melanogaster there are two gamma-tubulin genes, gammaTUB23C and gammaTUB37C. Here we report the cytological and molecular characterization of the 37C isoform...
- Centriole and centrosome dynamics during the embryonic cell cycles that follow the formation of the cellular blastoderm in DrosophilaG Callaini
Department of Evolutionary Biology, University of Siena, Italy
Exp Cell Res 234:183-90. 1997..The centrosomal material, as recognized by antibodies against CP190 and gamma-tubulin, does not show the typical shape changes observed in syncytial embryos, but remains compact throughout mitosis...
- gamma-Tubulin is transiently associated with the Drosophila oocyte meiotic apparatusM G Riparbelli
Department of Evolutionary Biology, University of Siena, Italy
Eur J Cell Biol 75:21-8. 1998..This center contained gamma-tubulin and CP190 antigens and nucleated a transient array of radial microtubules...
- Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffoldsM Moritz
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 0448, USA
J Cell Biol 142:775-86. 1998..2 M KI generates salt-resistant scaffolds that lack the centrosomal proteins CP190, CP60, centrosomin, and gamma-tubulin. To clarify the role of these proteins in microtubule nucleation by centrosomes and to identify additional ..
- Centrosomes and microtubule organisation during Drosophila developmentC Gonzalez
European Molecular Biology Laboratory Meyerhofstrasse 1, Germany
J Cell Sci 111:2697-706. 1998..In this review we present some of the many examples which illustrate how centrosomes and microtubule organisation changes through development in Drosophila and discuss some of its implications...
- The POLO kinase is required at multiple stages during spermatogenesis in Drosophila melanogasterS Herrmann
Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, P 4150 Porto, Portugal
Chromosoma 107:440-51. 1998..Immunostaining of polo mutant cells with alpha-tubulin shows several abnormalities of the meiotic spindle, including a significantly reduced central spindle...
- Spindle assembly in Drosophila neuroblasts and ganglion mother cellsS Bonaccorsi
Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, 00185 Rome, Italy
Nat Cell Biol 2:54-6. 2000
- In vivo localisation of the mitotic POLO kinase shows a highly dynamic association with the mitotic apparatus during early embryogenesis in DrosophilaT Moutinho-Santos
Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal
Biol Cell 91:585-96. 1999..Furthermore, the wide distribution of the GFP-POLO protein to all compartments of the mitotic apparatus provides a valuable tool for future studies on cell cycle during development...
- The Drosophila wispy gene is required for RNA localization and other microtubule-based events of meiosis and early embryogenesisA E Brent
Department of Biological Sciences, Columbia University, New York, New York 10027, USA
Genetics 154:1649-62. 2000..We propose that the wispy gene product functions directly in several microtubule-based events in meiosis and early embryogenesis and speculate about its possible mode of action...
- Functional analysis of the Drosophila diaphanous FH protein in early embryonic developmentK Afshar
Department of Biology, Center for Molecular Genetics, University of California, San Diego, La Jolla CA 92093 0634, USA
Development 127:1887-97. 2000..Based on known biochemical functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecules and actin organizers at specific phases of the cell cycle...
- Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of DrosophilaM G Riparbelli
University of Siena, Department of Evolutionary Biology, Via Mattioli 4, I 53100 Siena, Italy
J Cell Sci 113:3341-50. 2000..Migration of the female pronucleus did not take place and so a gonomeric spindle could not form. We discuss these findings in relation to the known roles of polo like kinases in regulating the behaviour of MTOCs...
- The fusome organizes the microtubule network during oocyte differentiation in DrosophilaN C Grieder
Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
Development 127:4253-64. 2000..During this same period, discrete foci rich in gamma tubulin that very probably correspond to migrating cystocyte centrosomes also associate with the fusome, first on the ..
- Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localizationV Barbosa
Cancer Research Campaign, Cell Cycle Genetics Group, University of Cambridge, Department of Genetics, Cambridge, UK
Genes Dev 14:3126-39. 2000We have cloned the Drosophila gene discs degenerate-4 (dd4) and find that it encodes a component of the gamma-tubulin ring complex (gammaTuRC) homologous to Spc98 of budding yeast...
- Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunitsR N Gunawardane
Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
J Cell Biol 151:1513-24. 2000The gamma-tubulin ring complex (gammaTuRC) is important for microtubule nucleation from the centrosome...
- Zygotic development without functional mitotic centrosomesT L Megraw
Department of Biology and Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA
Curr Biol 11:116-20. 2001..These results challenge long-held assumptions that the centrosome and the astral microtubules emanating from it are essential for development and are required specifically for spindle orientation during asymmetric cell divisions...
- The drosophila protein asp is involved in microtubule organization during spindle formation and cytokinesisJ G Wakefield
Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Rome, Italy
J Cell Biol 153:637-48. 2001..both meiotic and mitotic divisions of asp mutants, microtubule nucleation occurs from the centrosome, and gamma-tubulin localizes correctly. However, spindle pole focusing and organization are severely affected...
- Differential properties of the two Drosophila gamma-tubulin isotypesB Raynaud-Messina
Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Toulouse France
Eur J Cell Biol 80:643-9. 2001..The two gamma-tubulins exhibit distinct biochemical properties. Gamma-tub23C is present in the soluble gamma-tubulin small complexes (10S) and gamma-tubulin big complexes (35S) and is loosely associated to the cytoskeleton...
- A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in DrosophilaMaria Giovanna Riparbelli
University of Siena, Department of Evolutionary Biology, Via Mattioli 4, I 53100 Siena, Italy
J Cell Sci 115:913-22. 2002..Upon fertilisation, Asp is also recruited to the MTOC that nucleates the sperm aster. Asp is required for growth of the microtubules of the sperm aster, which in asp mutants remains diminutive and so prevents migration of the pronuclei...
- Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosisDaniela Berdnik
Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, A 1030, Vienna, Austria
Curr Biol 12:640-7. 2002..What triggers the asymmetric localization of Numb at the onset of mitosis is unclear...
- subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogasterKelly L Giunta
Waksman Institute and Department of Genetics, Rutgers University, Piscataway, New Jersey 08854, USA
Genetics 160:1489-501. 2002..sub is also required for the early embryonic divisions but is otherwise dispensable for most mitotic divisions...
- Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posteriorByeong Jik Cha
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Nat Cell Biol 4:592-8. 2002....
- Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesisFrank Schnorrer
Max Planck Institut fur Entwicklungsbiologie, Abteilung Genetik, Spemannstr 35, 72076, Tubingen, Germany
Dev Cell 3:685-96. 2002..They encode maternal gamma-Tubulin37C (gammaTub37C) and gamma-tubulin ring complex protein 75 (Dgrip75), both of which are gamma-tubulin ring complex components...
- Characterization of a new gammaTuRC subunit with WD repeatsRuwanthi N Gunawardane
Carnegie Institution of Washington Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
Mol Biol Cell 14:1017-26. 2003The gamma-tubulin ring complex (gammaTuRC), consisting of multiple protein subunits, can nucleate microtubule assembly. Although many subunits of the gammaTuRC have been identified, a complete set remains to be defined in any organism...
- Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cellsYasuhiko Terada
Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
J Cell Biol 162:757-63. 2003..and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of gamma-tubulin and other centrosomal components to the centrosome...
- Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAPShin Ichi Kawaguchi
Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA
Mol Biol Cell 15:37-45. 2004..this novel Drosophila centrosome protein, centrosome protein of 309 kDa (CP309), cofractionates with the gamma-tubulin ring complex and the centrosome-complementing activity...
- The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosisMaruxa Martinez-Campos
Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, University of Cambridge, England, UK
J Cell Biol 165:673-83. 2004..Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies...
- Spindle pole organization in Drosophila S2 cells by dynein, abnormal spindle protein (Asp), and KLP10ASandra Morales-Mulia
Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California Davis, Davis, CA 95616, USA
Mol Biol Cell 16:3176-86. 2005..These results extend recent findings from Xenopus extracts to Drosophila cultured cells and suggest that common pathways contribute to spindle pole organization and length determination...
- The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytesJ K Jang
Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
Mol Biol Cell 16:4684-94. 2005..We propose that Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle...
- Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubulesTeresa P Barros
The Wellcome Trust Cancer Research UK Gurdon Institute, Department of Genetics, Cambridge CB2 1QN, England, UK
J Cell Biol 170:1039-46. 2005..This may explain why centrosomes are such dominant sites of MT assembly during mitosis...
- The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatusNathalie Colombié
Centre de Recherche en Pharmacologie Santé, Unité Mixte de Recherche 2587, Centre National de la Recherche Scientifique Pierre Fabre, Institut de Sciences et Technologies du Medicament de Toulouse, 31400 Toulouse, France
Mol Biol Cell 17:272-82. 2006Gamma-tubulin, a protein critical for microtubule assembly, functions within multiprotein complexes. However, little is known about the respective role of gamma-tubulin partners in metazoans...
- The centrosome-nucleus complex and microtubule organization in the Drosophila oocyteJens Januschke
Institut Jacques Monod, Unité Mixte Recherche 7592, CNRS, Université Paris 6 et Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France
Development 133:129-39. 2006..Remarkably, the centrosomal components, gamma-tubulin and Drosophila pericentrin-like protein also strongly accumulate at the periphery of this nucleus...
- Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleationChristel Verollet
Centre de Recherche en Pharmacologie, santé, UMR 2587, Centre National de la Recherche Scientifique Pierre Fabre, Institut de Sciences et Technologies du Medicament de Toulouse, 31432 Toulouse, Cedex 4, France
J Cell Biol 172:517-28. 2006In metazoans, gamma-tubulin acts within two main complexes, gamma-tubulin small complexes (gamma-TuSCs) and gamma-tubulin ring complexes (gamma-TuRCs)...
- Revisiting the role of the mother centriole in centriole biogenesisA Rodrigues-Martins
Instituto Gulbenkian de Ciencia, Cell Cycle Regulation Laboratory, Rua da Quinta Grande, 6, P 2780 156 Oeiras, Portugal
Science 316:1046-50. 2007..The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly...
- Overexpressing centriole-replication proteins in vivo induces centriole overduplication and de novo formationNina Peel
The Gurdon Institute, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
Curr Biol 17:834-43. 2007..Here, we investigate the effects of overexpressing the three proteins known to be required for centriole replication in Drosophila-DSas-6, DSas-4, and Sak...
- Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1Jiuli Zhang
Department of Pharmacology, The Cecil and Ida Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9051, USA
Mol Biol Cell 18:4037-49. 2007..Cnn Motif 1 is necessary for proper recruitment of gamma-tubulin, D-TACC (the homolog of vertebrate transforming acidic coiled-coil proteins [TACC]), and Minispindles (Msps) to ..
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in DrosophilaChristiane Wiese
Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
Mol Biol Cell 19:368-77. 2008..extensively, the gamma-tubulin genes are developmentally regulated: the "maternal" gamma-tubulin isotype (named gammaTub37CD according to its location on the genetic map) is expressed in the ovary and is deposited in the egg, where it ..
- Augmin: a protein complex required for centrosome-independent microtubule generation within the spindleGohta Goshima
Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
J Cell Biol 181:421-9. 2008Since the discovery of gamma-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome...
- A multicomponent assembly pathway contributes to the formation of acentrosomal microtubule arrays in interphase Drosophila cellsGregory C Rogers
Department of Biology, Lineberger Comprehensive Cancer Center, and Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Biol Cell 19:3163-78. 2008..However, gamma-tubulin RNAi delays microtubule regrowth after depolymerization, suggesting that it may function partially redundantly ..
- Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosisNathalie Colombié
Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Edinburgh, UK
Development 135:3239-46. 2008..We propose that the two functions of Incenp are part of the mechanisms that compensate for the lack of centrosomes during meiotic spindle formation...
- Drosophila asterless and vertebrate Cep152 Are orthologs essential for centriole duplicationStephanie Blachon
Department of Biology and Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
Genetics 180:2081-94. 2008..The clear absence of several centriolar markers in mecD mutants suggests that Asl is critical early in centriole duplication...
- Drosophila Dgt6 interacts with Ndc80, Msps/XMAP215, and gamma-tubulin to promote kinetochore-driven MT formationElisabetta Bucciarelli
Istituto Pasteur Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del Consiglio Nazionale delle Ricerche CNR, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, P A Moro 5, 00185 Roma, Italy
Curr Biol 19:1839-45. 2009..Recent work has suggested that additional spindle MTs can be nucleated by gamma-tubulin ring complexes (gamma-TuRCs) that associate laterally with preexisting spindle MTs, leading to spindle ..
- Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole retention in Drosophila neuroblastsPaul T Conduit
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Curr Biol 20:2187-92. 2010..This ensures that the daughter centriole maintains its PCM and so its position at the apical cortex. Thus, the daughter centriole, rather than an "immortal" mother centriole, is ultimately retained in these stem cells...
- Anastral spindle assembly and γ-tubulin in Drosophila oocytesSharyn A Endow
Department of Cell Biology and Structural Biology and Biophysics Program, Duke University, USA
BMC Cell Biol 12:1. 2011..It is still uncertain whether γ-tubulin, a microtubule nucleator essential for mitotic spindle assembly and maintenance, plays a role...
- Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosomeJayachandran Gopalakrishnan
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Commun 2:359. 2011..In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome...
- A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyteRichard M Parton
Department of Biochemistry, University of Oxford, Oxford OX1 3QU, England, UK
J Cell Biol 194:121-35. 2011..Our findings explain the biased random posterior cargo movements in the oocyte that establish the germline and posterior...
- Gamma-tubulin is required for bipolar spindle assembly and for proper kinetochore microtubule attachments during prometaphase I in Drosophila oocytesStacie E Hughes
Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
PLoS Genet 7:e1002209. 2011..Previous studies of Drosophila oocytes failed to detect the native form of the germline-specific γ-tubulin (γTub37C) in meiosis I spindles, and genetic studies have yielded conflicting data regarding the role of γ..
- Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRCRichard F Reschen
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
Biol Open 1:422-9. 2012Dgp71WD/Nedd1 proteins are essential for mitotic spindle formation. In human cells, Nedd1 targets γ-tubulin to both centrosomes and spindles, but in other organisms the function of Dgp71WD/Nedd1 is less clear...
- Golgi outposts shape dendrite morphology by functioning as sites of acentrosomal microtubule nucleation in neuronsKassandra M Ori-McKenney
Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA
Neuron 76:921-30. 2012..This acentrosomal nucleation requires gamma-tubulin and CP309, the Drosophila homolog of AKAP450, and contributes to the complex microtubule organization within the ..
- Centrobin controls mother-daughter centriole asymmetry in Drosophila neuroblastsJ Januschke
Institute for Research in Biomedicine IRB Barcelona, Baldiri Reixac 10, 08028 Barcelona, Spain
Nat Cell Biol 15:241-8. 2013..They also reveal an interphase function for POLO in this process that seems to have co-opted part of the protein network involved in mitotic centrosome maturation...
- Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytesS A Endow
Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA
J Cell Sci 111:2487-95. 1998..gamma-Tubulin transiently localizes to the central spindle pole body, implying that the body acts as a microtubule nucleating ..
- Drosophila polo kinase is required for cytokinesisM Carmena
CRC Cell Cycle Group, Cancer Research Campaign Laboratories, Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland
J Cell Biol 143:659-71. 1998..We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis...
- Cytoplasmic dynein is required for the nuclear attachment and migration of centrosomes during mitosis in DrosophilaJ T Robinson
University of Minnesota, Department of Genetics, Cell Biology and Development, St Paul, Minnesota 55108 1095, USA
J Cell Biol 146:597-608. 1999..The disruption of these centrosome attachments in mutant embryos reveals a critical role for dynein function and centrosome positioning in the spatial organization of the syncytial cytoplasm of the developing embryo...
- Structure of the gamma-tubulin ring complex: a template for microtubule nucleationM Moritz
Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, 513 Parnassus Avenue, San Francisco, California 94143, USA
Nat Cell Biol 2:365-70. 2000The gamma-tubulin ring complex (gammaTuRC) is a protein complex of relative molecular mass approximately 2.2 x 10(6) that nucleates microtubules at the centrosome...
- Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeletonJ Bolivar
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Development 128:1889-97. 2001..Thus, centrosome migration is independent of the organisation of the microtubule cytoskeleton, and seems to depend instead on the polarity of the fusome...
- Asymmetric inheritance of mother versus daughter centrosome in stem cell divisionYukiko M Yamashita
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305 5329, USA
Science 315:518-21. 2007..The mother centrosome remains anchored near the niche while the daughter centrosome migrates to the opposite side of the cell before spindle formation...
- Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cellsBrigitte Raynaud-Messina
ISTMT, Centre de Recherche en Pharmacologie Santé, UMR 2587 CNRS P Fabre, 3 rue des Satellites, 31 400 Toulouse, France
J Cell Sci 117:5497-507. 2004The assembly of the mitotic spindle after depletion of the major gamma-tubulin isotype by RNA-mediated interference was assessed in the Drosophila S2 cell line...
- The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germlineNina Vogt
Max Planck Institute for Developmental Biology, Department of Genetics, Spemannstr 35, 72076 Tubingen, Germany
Development 133:3963-72. 2006The gamma-tubulin ring complex (gammaTuRC) forms an essential template for microtubule nucleation in animal cells...
- Germline self-renewal requires cyst stem cells and stat regulates niche adhesion in Drosophila testesJudith L Leatherman
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine Institute for Regenerative Medicine, University of Pennsylvania Philadelphia, USA
Nat Cell Biol 12:806-11. 2010..This work also reconciles key differences in GSC renewal between Drosophila testis and ovary niches, and highlights how a niche can coordinate the production of distinct lineages by having one stem cell type rely on a second...
- Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learningG M Bolwig
Cold Spring Harbor Laboratory, New York 11724, USA
Neuron 15:829-42. 1995..The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein...
- Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cellsA Debec
Groupe de Génétique Cellulaire et Moléculaire, Unité Associée du CNRS 1135, Universite Paris 6, France
J Cell Sci 108:2645-53. 1995The spindle pole localization of gamma-tubulin was compared in wild type and acentriolar cultured Drosophila cells using polyclonal antibodies specifically raised against the carboxy terminal amino acid sequence of Drosophila gamma-..
- Effects of Ddc cluster lethal alleles on ovary growth, attachment, and egg production in DrosophilaE McCrady
Department of Biology, University of North Carolina at Greensboro 27412
J Exp Zool 268:469-76. 1994..Heteroallelic heterozygotes of Bd7 x Bd4 also produced fully fertile ovaries, but no other heteroallelic combinations did so.(ABSTRACT TRUNCATED AT 250 WORDS)..
- A mutation (dosach) in Drosophila which affects aster formation and nuclear migration during cleavageS S Craig
School of Biological Sciences, Flinders University of South Australia, Australia
Biol Cell 87:45-54. 1996..Confocal microscopy has been used to correlate organization of microtubules, centrosomal proteins and chromosomes in wild-type and dosach (dos) embryos...
- Heterotrimeric G proteins direct two modes of asymmetric cell division in the Drosophila nervous systemM Schaefer
Research Institute of Molecular Pathology I M P, Dr Bohr Gasse 7, 1030 Vienna, Austria
Cell 107:183-94. 2001..Thus, asymmetric activation of heterotrimeric G proteins by a receptor-independent mechanism may orient asymmetric cell divisions in different cell types...
- Centrosomal TACCticsFanni Gergely
Wellcome CR UK Institute, Department of Pharmacology, University of Cambridge, UK
Bioessays 24:915-25. 2002..In this review, I summarise what we already know of this protein family and will use the TACC proteins to illustrate the many facets that centrosomes have developed during the course of evolution...
- Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosisElisabetta Bucciarelli
Istituto Pasteur Fondazione Cenci Bolognetti, 00185 Rome, Italy
J Cell Biol 160:993-9. 2003..This suggests that the association of Aurora B with chromosomes is not a prerequisite for its accumulation at the central spindle, or for its function during cytokinesis...
- Heterotrimeric G proteins regulate daughter cell size asymmetry in Drosophila neuroblast divisionsNaoyuki Fuse
Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, Chuou ku, Kobe 650 0047, Japan
Curr Biol 13:947-54. 2003..Furthermore, the multiple equal cleavages of G beta mutant neuroblasts accompany neural defects; this finding suggests indispensable roles of eccentric division in assuring the stem cell properties of neuroblasts...
- The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1Matthias G Fischer
BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
Curr Biol 14:2019-24. 2004..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
- The centrosomal protein CP190 is a component of the gypsy chromatin insulatorChi Yun Pai
Department of Biology, Johns Hopkins University, 3400 N Charles Street, Baltimore, Maryland 21218, USA
Mol Cell 16:737-48. 2004..The results suggest that endogenous gypsy insulators contain binding sites for CP190, which is essential for insulator function, and may or may not contain binding sites for Su(Hw) and Mod(mdg4)2.2...
- Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesisJason Stumpff
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
Curr Biol 14:2143-8. 2004..These findings modify previous models about cell cycle regulation in syncytial embryos and demonstrate that Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase...
- Centrosome inheritance in the male germ line of Drosophila requires hu-li tai-shao functionPatricia G Wilson
Department of Biology, Georgia State University, MSC 8L0389, 33, Gilmer Street SE, Unit 8, Atlanta, GA 30303 3088, USA
Cell Biol Int 29:360-9. 2005..Collectively, these results indicate that hts function is necessary for centrosome inheritance in spermatocytes as well as for male fertility...
- The centrosomal protein CP190 regulates myosin function during early Drosophila developmentS Chodagam
The Gurdon Institute, Department of Genetics, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
Curr Biol 15:1308-13. 2005..Thus, CP190 has distinct nuclear and centrosomal functions, and it provides a crucial link between the centrosome/MT and actin/myosin cytoskeletal systems in early embryos...
- Drosophila mars is required for organizing kinetochore microtubules during mitosisChing Po Yang
Department of Life Science, Tunghai University, Taichung, Taiwan, ROC
Exp Cell Res 314:3209-20. 2008..In summary, this study has extended our previous study of Mars in cell cycle regulation and provided further evidence showing that Mars is required for the assembly of kinetochore microtubules...
- Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family memberC A Callahan
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, California 92186, USA
Nature 376:171-4. 1995..In drl mutant embryos these neurons fail to make the correct pathway choices. Our results provide evidence for receptor protein-tyrosine kinase involvement in key aspects of neuronal pathway recognition...
- The genetic and molecular organization of the Dopa decarboxylase gene cluster of Drosophila melanogasterD G Stathakis
Department of Biology, University of Virginia, Charlottesville 22903 2477, USA
Genetics 141:629-55. 1995....
- The Wilhelmine E. Key 1992 Invitational lecture. Phenotypic analysis of the Dopa decarboxylase gene cluster mutants in Drosophila melanogasterT R Wright
University of Virginia, Department of Biology, Charlottesville 22903 2477, USA
J Hered 87:175-90. 1996..Thus, the Ddc gene cluster represents in higher eukaryotes an unusual example of a large cluster of functionally related genes involved in a common physiological process...
- Protein phosphatase 4 is an essential enzyme required for organisation of microtubules at centrosomes in Drosophila embryosN R Helps
Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK
J Cell Sci 111:1331-40. 1998..A reduction in the staining of gamma-tubulin at centrosomes in cmm embryos suggests a conformational change or relocation of this protein, which is known to ..
- Cytological characterisation of the mutant phenotypes produced during early embryogenesis by null and loss-of-function alleles of the gammaTub37C gene in DrosophilaS Llamazares
Cell Biology Programme, EMBL, Meyerhofstr, Heidelberg, Germany
J Cell Sci 112:659-67. 1999We have studied the mutant phenotypes brought about during early embryogenesis by mutation in the gammaTub37C gene, one of the two isoforms of gamma-tubulin that have been identified in Drosophila...
- Rotation and asymmetry of the mitotic spindle direct asymmetric cell division in the developing central nervous systemJ A Kaltschmidt
Wellcome CRC Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
Nat Cell Biol 2:7-12. 2000..This observation contradicts the widely held hypothesis that the cleavage furrow is always placed midway between the two centrosomes...
- DNA defects target the centrosomeT T Su
Nat Cell Biol 2:E28-9. 2000..These defects may result from the workings of a surveillance mechanism that acts to cull irreparable nuclei...
- Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebratesB M Lange
Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
EMBO J 19:1252-62. 2000..This suggests that another role of Hsp90 might be to ensure proper centrosome function...
- Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryosV E Foe
Department of Zoology, University of Washington, Seattle WA 98195 1800, USA
Development 127:1767-87. 2000....
- Patterns of cell division and expression of asymmetric cell fate determinants in postembryonic neuroblast lineages of DrosophilaJ Ceron
Instituto de Neurociencias, Universidad Miguel Hernandez y CSIC, Alicante, 03550, Spain
Dev Biol 230:125-38. 2001..vi) Prospero is transiently expressed in one of the two sibling GCs generated by the division of GMCs. The implications of these results on cell fate specification and differentiation of adult brain neurons are discussed...
- The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germlineC Vied
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, Alabama 35294, USA
Development 128:2649-60. 2001..This is the first demonstration that downstream components of the Hedgehog signaling pathway regulate a target other than Cubitus interruptus...
- Proteolytic cleavage of the THR subunit during anaphase limits Drosophila separase functionAlf Herzig
Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
Genes Dev 16:2443-54. 2002....
- Antizyme is a target of sex-lethal in the Drosophila germline and appears to act downstream of hedgehog to regulate sex-lethal and cyclin BCynthia Vied
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
Dev Biol 253:214-29. 2003..This regulation of Sex-lethal by gutfeeling appears to occur downstream of the Hedgehog signal. We also show that Hedgehog, Gutfeeling, and Sex-lethal function to regulate Cyclin B, providing a link between Sex-lethal and mitosis...
- gamma-Tubulin function during female germ-cell development and oogenesis in DrosophilaGaia Tavosanis
Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Proc Natl Acad Sci U S A 100:10263-8. 2003..To gain a better understanding of their molecular nature, we have studied the centrosomal component gamma-tubulin during Drosophila oogenesis...