Est-6

Summary

Gene Symbol: Est-6
Description: Esterase 6
Alias: CG6917, Dmel\CG6917, EST-5, EST-6, EST6, Est, Est 6, Est-5, Est-5B, Est-D, Est6, est-6, est6, CG6917 gene product from transcript CG6917-RB, CG6917-PA, CG6917-PB, Est-6-PA, Est-6-PB, Esterase-6, Esterase6, Stress-inducible humoral factor turandot A, carboxyl ester hydrolase, esterase 6
Species: fruit fly

Top Publications

  1. ncbi An episode of accelerated amino acid change in Drosophila esterase-6 associated with a change in physiological function
    J G Oakeshott
    Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australian Capital Territory
    Genetica 110:231-44. 2000
  2. ncbi Positive and negative selection in the beta-esterase gene cluster of the Drosophila melanogaster subgroup
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, 321 Steinhaus Hall, Irvine, CA 92697 2525, USA
    J Mol Evol 62:496-510. 2006
  3. pmc Structural comparison of two esterases from Drosophila mojavensis isolated by immunoaffinity chromatography
    J Pen
    Biochem J 238:691-9. 1986
  4. ncbi Entropy and GC Content in the beta-esterase gene cluster of the Drosophila melanogaster subgroup
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, USA
    Mol Biol Evol 22:2063-72. 2005
  5. ncbi The beta-esterase gene cluster of drosophila melanogaster: is psiEst-6 a pseudogene, a functional gene, or both?
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697 2525, USA
    Genetica 121:165-79. 2004
  6. ncbi Molecular population genetics of the beta-esterase gene cluster of Drosophila melanogaster
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697 2525, USA
    J Genet 82:115-31. 2003
  7. ncbi Associations of esterase 6 allozyme and activity variation with reproductive fitness in Drosophila melanogaster
    M Saad
    CSIRO Division of Entomology, Canberra ACT, Australia
    Genetica 94:43-56. 1994
  8. ncbi The expression of esterase S gene of Drosophila virilis in Drosophila melanogaster
    P V Sergeev
    Engelhardt Institute of Molecular Biology, Russian Acad Sci, Moscow
    FEBS Lett 360:194-6. 1995
  9. pmc Nucleotide polymorphism in the Est6 promoter, which is widespread in derived populations of Drosophila melanogaster, changes the level of Esterase 6 expressed in the male ejaculatory duct
    Wendy A Odgers
    CSIRO Entomology, Canberra, 2601 Australia
    Genetics 162:785-97. 2002
  10. ncbi Esterase 6 and reproduction in Drosophila melanogaster
    R C Richmond
    Science 207:1483-5. 1980

Research Grants

  1. Comparative Genomics of Seminal Fluid Proteins
    ANTHONY FIUMERA; Fiscal Year: 2005

Scientific Experts

  • M T Hamblin
  • J G Oakeshott
  • Timothy B Sackton
  • Charles Robin
  • Andrea Riebler
  • Anthony C Fiumera
  • Xiang Yang Lou
  • R R Hudson
  • E S Balakirev
  • P Andolfatto
  • F J Ayala
  • J C Fay
  • Richard M Kliman
  • Tracey Chapman
  • C Zapata
  • P Capy
  • Bernardo Lemos
  • Mohamed A F Noor
  • Christopher W Coppin
  • Faisal Younus
  • Ruth Birner-Gruenberger
  • Wendy A Odgers
  • M J Healy
  • Peter L Morrell
  • Margaret C Bloch Qazi
  • Françoise Bozzolan
  • Stephen L Pearce
  • Thomas Chertemps
  • Gunjan Pandey
  • Robyn J Russell
  • Martine Maïbèche-Coisne
  • Gerald N Rechberger
  • Philip Hehlert
  • P M Campbell
  • Albin Hermetter
  • Julia Lange
  • Iris Bickmeyer
  • Manfred Kollroser
  • Ronald P Kühnlein
  • P P Lopez
  • M M Dumancic
  • R C Richmond
  • R J Russell
  • P V Sergeev
  • A Navarro-Sabate
  • O A Bubliy
  • J Karotam
  • D M Neubaum
  • A G Imasheva
  • N A Tamarina
  • Donna M Toleno
  • Karen E Lundy
  • O A Bubliĭ
  • Michael T Clegg
  • D J Begun
  • Mariana F Wolfner
  • Yael Heifetz
  • M Z Ludwig
  • L I Korochkin
  • A R Templeton
  • E Hasson
  • M A Myers
  • Charles F Aquadro
  • J H McDonald
  • Marion J Healy
  • L Sanchez
  • C Claudianos
  • M F Ruiz
  • P Esponda
  • J F Santaren
  • E J Crone
  • R L Harcourt
  • B D Hammock
  • W A Odgers
  • M Aguade
  • G P Georgiev
  • B A Kalabushkin
  • C Segarra
  • M F Wolfner
  • V N Totskiĭ
  • G M Smith
  • J P Brady
  • W Rumball
  • K A Crandall
  • M Saad
  • R Parkash
  • A Y Game
  • A Cao
  • D V Korotkov
  • A Z el-Abidin Salam

Detail Information

Publications95

  1. ncbi An episode of accelerated amino acid change in Drosophila esterase-6 associated with a change in physiological function
    J G Oakeshott
    Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, Australian Capital Territory
    Genetica 110:231-44. 2000
    ..There are also three changes in glycosylation status. One region predicted to lie on the protein surface which becomes markedly more hydrophilic is proposed to be the ancestral dimerisation site that is lost in the transitional branch...
  2. ncbi Positive and negative selection in the beta-esterase gene cluster of the Drosophila melanogaster subgroup
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, 321 Steinhaus Hall, Irvine, CA 92697 2525, USA
    J Mol Evol 62:496-510. 2006
    We examine the pattern of molecular evolution of the beta-esterase gene cluster, including the Est-6 and psiEst-6 genes, in eight species of the Drosophila melanogaster subgroup...
  3. pmc Structural comparison of two esterases from Drosophila mojavensis isolated by immunoaffinity chromatography
    J Pen
    Biochem J 238:691-9. 1986
    ..Although both enzymes exhibit little immunological cross-reactivity, their amino acid compositions show few significant differences and their N-terminal sequences are largely identical, which clearly show their common origin...
  4. ncbi Entropy and GC Content in the beta-esterase gene cluster of the Drosophila melanogaster subgroup
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA, USA
    Mol Biol Evol 22:2063-72. 2005
    We perform spectral entropy and GC content analyses in the beta-esterase gene cluster, including the Est-6 gene and the psiEst-6 putative pseudogene, in seven species of the Drosophila melanogaster species subgroup...
  5. ncbi The beta-esterase gene cluster of drosophila melanogaster: is psiEst-6 a pseudogene, a functional gene, or both?
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697 2525, USA
    Genetica 121:165-79. 2004
    ..We investigate, as a model case, the beta-esterase gene cluster of Drosophila melanogaster that includes the Est-6 gene and the psiEst-6 putative pseudogene...
  6. ncbi Molecular population genetics of the beta-esterase gene cluster of Drosophila melanogaster
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697 2525, USA
    J Genet 82:115-31. 2003
    We have investigated nucleotide polymorphism at the beta-esterase gene cluster including the Est-6 gene and psiEst-6 putative pseudogene in four samples of Drosophila melanogaster derived from natural populations of southern Africa (..
  7. ncbi Associations of esterase 6 allozyme and activity variation with reproductive fitness in Drosophila melanogaster
    M Saad
    CSIRO Division of Entomology, Canberra ACT, Australia
    Genetica 94:43-56. 1994
    Previous studies have shown that the esterase 6 (EST6) enzyme of D...
  8. ncbi The expression of esterase S gene of Drosophila virilis in Drosophila melanogaster
    P V Sergeev
    Engelhardt Institute of Molecular Biology, Russian Acad Sci, Moscow
    FEBS Lett 360:194-6. 1995
    ..The comparison between deduced amino-acid sequences of the esterase S of Drosophila virilis and esterase 6 of Drosophila melanogaster was performed. These sequences revealed 50% homology.
  9. pmc Nucleotide polymorphism in the Est6 promoter, which is widespread in derived populations of Drosophila melanogaster, changes the level of Esterase 6 expressed in the male ejaculatory duct
    Wendy A Odgers
    CSIRO Entomology, Canberra, 2601 Australia
    Genetics 162:785-97. 2002
    Previous analysis of an Australian population of D. melanogaster revealed two predominant Est6 promoter haplotypes, P1 and P7...
  10. ncbi Esterase 6 and reproduction in Drosophila melanogaster
    R C Richmond
    Science 207:1483-5. 1980
    A nonspecific carboxylesterase (esterase 6) of Drosophila melanogaster shows greater activity in adult males than in females and is highly concentrated in the anterior ejaculatory duct of the reproductive tract of the male...
  11. ncbi Evolutionary genetics of Drosophila esterases
    J G Oakeshott
    CSIRO Division of Entomology, Canberra ACT, Australia
    Genetica 90:239-68. 1993
    ..Sequence differences between two other esterases, the EST6 carboxyl esterase and acetylcholinesterase, have been interpreted against the consensus super-secondary structure ..
  12. ncbi Identification of a juvenile hormone esterase gene by matching its peptide mass fingerprint with a sequence from the Drosophila genome project
    P M Campbell
    Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, PO Box 1700, ACT 2601, Canberra, Australia
    Insect Biochem Mol Biol 31:513-20. 2001
    ..This sequence is in agreement with the Drosophila genome project's prediction except that the sixth predicted intron is not removed; instead there is a stop codon followed by a polyadenylation signal and a polyA tail...
  13. pmc Localization of sequences controlling the spatial, temporal, and sex-specific expression of the esterase 6 locus in Drosophila melanogaster adults
    M Z Ludwig
    Department of Biology, University of South Florida, Tampa 33620
    Proc Natl Acad Sci U S A 90:6233-7. 1993
    The esterase 6 gene (Est-6) of Drosophila melanogaster is expressed in a variety of tissues that differ between larval and adult stages and among related species...
  14. pmc Regulation of tissue-specific expression of the esterase S gene in Drosophila virilis
    P V Sergeev
    Engelhardt Institute of Molecular Biology, Russian Acad Sci, Moscow
    Nucleic Acids Res 21:3545-51. 1993
    ..Point mutations destroying the three ORFs completely eliminate the inhibitory effect. Hence tissue-specific expression of the estS gene may depend on control at the level of transcription, promoter selection and translation...
  15. ncbi Conservation and change in structural and 5' flanking sequences of esterase 6 in sibling Drosophila species
    J Karotam
    Molecular Biology and Physiology Section, CSIRO Division of Entomology, Canberra A C T, Australia
    Genetica 88:11-28. 1993
    b>Esterase 6 (Est-6/EST6) is the major beta-carboxylesterase in D. melanogaster and its siblings D. simulans and D. mauritiana...
  16. ncbi Characterization of the EstP protein in Drosophila melanogaster and its conservation in drosophilids
    M M Dumancic
    CSIRO Division of Entomology, Canberra, Australia
    Biochem Genet 35:251-71. 1997
    The beta-esterase cluster of D. melanogaster comprises two tandemly duplicated genes. Est6 encodes the well-characterized 5' gene, but the product of the second gene, denoted EstP, had not previously been identified...
  17. pmc Divergent and conserved features in the spatial expression of the Drosophila pseudoobscura esterase-5B gene and the esterase-6 gene of Drosophila melanogaster
    N A Tamarina
    Division of Vascular Surgery, Department of Surgery, Northwestern University Medical School, 251 East Chicago Avenue, Suite 626, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 94:7735-41. 1997
    The regulatory regions of homologous genes encoding esterase 6 (Est-6) of Drosophila melanogaster and esterase 5B (Est-5B) of Drosophila pseudoobscura show very little similarity...
  18. pmc Is esterase-P encoded by a cryptic pseudogene in Drosophila melanogaster?
    E S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine 92697 2525, USA
    Genetics 144:1511-8. 1996
    We have amplified and sequenced the gene encoding Esterase-P (Est-P) in 10 strains of Drosophila melanogaster. Three premature termination codons occur in the coding region of the gene in two strains...
  19. ncbi Levels of naturally occurring DNA polymorphism correlate with recombination rates in D. melanogaster
    D J Begun
    Section of Genetics and Development, Cornell University, Ithaca, New York 14853 2703
    Nature 356:519-20. 1992
    ..Hitch-hiking thus seems to occur over a large fraction of the Drosophila genome and may constitute a major constraint on levels of genetic variation in nature...
  20. ncbi An evolutionary model for the duplication and divergence of esterase genes in Drosophila
    J P Brady
    Department of Biology, University of South Florida, Tampa 33620
    J Mol Evol 34:506-21. 1992
    The esterase 5 (Est-5 = gene, EST 5 = protein) enzyme in Drosophila pseudoobscura is encoded by one of three paralogous genes, Est-5A, Est-5B, and Est-5C, that are tightly clustered on the right arm of the X chromosome...
  21. pmc Molecular analysis of evolutionary changes in the expression of Drosophila esterases
    J P Brady
    Department of Biology, Indiana University, Bloomington 47405
    Proc Natl Acad Sci U S A 87:8217-21. 1990
    ..to assess the evolutionary basis for differences in the sex- and tissue-specific expression of the esterase 5 (EST 5) enzyme in D. pseudoobscura relative to its homologue in D. melanogaster, EST 6...
  22. ncbi Biochemical and physiological studies of soluble esterases from Drosophila melanogaster
    M J Healy
    CSIRO Division of Entomology, Canberra, ACT, Australia
    Biochem Genet 29:365-88. 1991
    ..Carboxylesterases are distributed across a wide range of tissues, but subclass 1 cholinesterases are generally associated with neural or neurosecretory tissues and subclass 2 cholinesterases with digestive tissues...
  23. ncbi Molecular analysis of duplicated esterase genes in Drosophila melanogaster
    C Collet
    Department of Biology, Indiana University, Bloomington 47405
    Mol Biol Evol 7:9-28. 1990
    Genomic clones containing sequences homologous to an esterase 6 (Est-6) cDNA clone were isolated from a library of Drosophila melanogaster DNA...
  24. pmc Glutactin, a novel Drosophila basement membrane-related glycoprotein with sequence similarity to serine esterases
    P F Olson
    Molecular Biology Institute, University of California, Los Angeles 90024 1570
    EMBO J 9:1219-27. 1990
    ..Several similarities with mammalian entactin caused our previous, preliminary mention of glutactin as a putative Drosophila entactin, but sequence comparison now shows them to be different proteins...
  25. ncbi Cloning of the esterase-5 locus from Drosophila pseudoobscura and comparison with its homologue in D. melanogaster
    J P Brady
    Department of Biology, Indiana University
    Mol Biol Evol 7:525-46. 1990
    ..by P-element transformation has demonstrated that Est-5B encodes an enzyme with the same physical properties as EST 5 in D. pseudoobscura...
  26. ncbi Carboxyl/cholinesterases: a case study of the evolution of a successful multigene family
    J G Oakeshott
    CSIRO Entomology, Canberra ACT 2601, Australia
    Bioessays 21:1031-42. 1999
    ..Furthermore, we postulate that the versatility of the substrate binding motifs underpins the recruitment of several family members to additional noncatalytic signal transduction functions...
  27. pmc Distribution of nonrandom associations between pairs of protein loci along the third chromosome of Drosophila melanogaster
    Carlos Zapata
    Departamento de Genetica, Universidad de Santiago, 15782 Santiago de Compostela, Spain
    Genetics 161:1539-50. 2002
    ..Our findings also suggest that GD may be a useful tool for discovering networks of functionally interacting proteins...
  28. ncbi Studies of esterase 6 in Drosophila melanogaster. I. The genetics of a posttranslational modification
    B J Cochrane
    Biochem Genet 17:167-83. 1979
    A locus has been found, an allele of which causes a modification of some allozymes of the enzyme esterase 6 in Drosophila melanogaster...
  29. doi Identification of candidate odorant degrading gene/enzyme systems in the antennal transcriptome of Drosophila melanogaster
    Faisal Younus
    CSIRO Ecosystems Sciences, Black Mountain, Clunies Ross Street, Canberra, ACT 0200, Australia Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    Insect Biochem Mol Biol 53:30-43. 2014
    ..Our findings do not preclude the possibility of odorant degrading functions for other antennally expressed esterases, P450s, GSTs and UGTs but, if so, they suggest that these enzymes also have additional functions in other tissues...
  30. ncbi The Drosophila melanogaster X-linked mfs(1)6E locus is required for production of normal seminal fluid by the male accessory glands
    P P Lopez
    Centro de Investigaciones Biologicas, Velazquez 144, Madrid, 28006, Spain
    Exp Cell Res 267:1-12. 2001
    ..Mutant male accessory glands show notable abnormalities, connected with glandular secretion as well as qualitative and quantitative differences in their protein content...
  31. ncbi [Genetic differentiation of three quantitative characters and natural populations of Drosophila melanogaster of Eastern Europe and Central Asia]
    O A Bubliĭ
    Genetika 30:230-6. 1994
    ..Productivity was significantly higher in populations of eastern Europe as compared to those of Central Asia. Selective mechanisms of maintenance of clinal variation in natural Drosophila populations are discussed...
  32. pmc Population genomic inferences from sparse high-throughput sequencing of two populations of Drosophila melanogaster
    Timothy B Sackton
    Department of Organismic and Evolutionary Biology, Harvard University, Boston, MA, USA
    Genome Biol Evol 1:449-65. 2009
    ..Continued development of methods for statistical inference of shallow-depth genome-wide sequencing data will allow such sparse, partial data sets to become the norm in the emerging field of population genomics...
  33. pmc A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation
    A R Templeton
    Department of Biology, Washington University, St Louis, Missouri 63130
    Genetics 132:619-33. 1992
    ..We apply this estimation procedure to three data sets to illustrate varying degrees of cladogram ambiguity and recombination...
  34. ncbi Detecting non-neutral heterogeneity across a region of DNA sequence in the ratio of polymorphism to divergence
    J H McDonald
    Department of Biology, University of Delaware, Newark 19716, USA
    Mol Biol Evol 13:253-60. 1996
    ..By simulating neutral evolution and comparing the observed number of runs to the simulations, a statistical test is possible which does not require any a priori decisions about subdivision...
  35. ncbi Pseudogenes: are they "junk" or functional DNA?
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697 2525, USA
    Annu Rev Genet 37:123-51. 2003
    ..We agree with the proposal that pseudogenes be considered as potogenes, i.e., DNA sequences with a potentiality for becoming new genes...
  36. pmc Birth and death of genes and functions in the beta-esterase cluster of Drosophila
    Charles Robin
    Department of Genetics, University of Melbourne, Parkville, Vic, Australia
    J Mol Evol 69:10-21. 2009
    ..This is reminiscent of the high frequency of null alleles observed in one of the beta-esterase genes (Est7/EstP) of D. melanogaster...
  37. pmc Deleterious background selection with recombination
    R R Hudson
    Department of Ecology and Evolutionary Biology, University of California, Irvine 92717, USA
    Genetics 141:1605-17. 1995
    ..If considerably smaller selection coefficients are assumed, the low observed levels of variation at the tips of the third chromosome are consistent with the background selection model...
  38. doi Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster α-Esterase-7
    Ruth Birner-Gruenberger
    Research Group Functional Proteomics, Institute of Pathology, and Proteomics Core Facility, Center of Medical Research, Medical University of Graz, Stiftingtalstrasse 24, 8010 Graz, Austria
    Insect Biochem Mol Biol 42:220-9. 2012
    ..It also offers an entry point for in vivo structure-function analyses of α-Esterase-7, which is of central importance for naturally occurring insecticide resistance in wild populations of various dipteran insect species...
  39. ncbi Testing the neutral theory of molecular evolution with genomic data from Drosophila
    Justin C Fay
    Committee on Genetics, University of Chicago, Chicago, Illinois 60637, USA
    Nature 415:1024-6. 2002
    ..A higher A/S ratio of divergence than of polymorphism is also observed in other species, which suggests a rate of adaptive evolution that is far higher than permitted by the neutral theory of molecular evolution...
  40. ncbi Geographic variation of six allozyme loci in Drosophila melanogaster: an analysis of data from different continents
    O A Bubliy
    Vavilov Institute of General Genetics, Moscow, Russia
    Hereditas 130:25-32. 1999
    ..The analyzed loci were Adh, Odh, Gpdh, G6pd, Pgd, and Est-6, which have been previously reported by other authors to show latitudinal variation in North America, Eurasia and ..
  41. pmc Mated Drosophila melanogaster females require a seminal fluid protein, Acp36DE, to store sperm efficiently
    D M Neubaum
    Department of Molecular Biology, Cornell University, Ithaca, New York 14853 2703, USA
    Genetics 153:845-57. 1999
    ..Acp36DE associates tightly with sperm. Our results indicate that Acp36DE is essential for the initial storage of sperm, and that it may also influence the arrangement and retention of stored sperm...
  42. pmc Contrasting patterns of nucleotide sequence variation at the glucose dehydrogenase (Gld) locus in different populations of Drosophila melanogaster
    M T Hamblin
    Section of Genetics and Development, Cornell University, Ithaca, NY14853, USA
    Genetics 145:1053-62. 1997
    ..The difference in patterns of variation across these population samples suggests that selection on Gld (or the Gld region) has been different in the Chinese population than in the other three...
  43. pmc Contrasting histories of three gene regions associated with In(3L)Payne of Drosophila melanogaster
    E Hasson
    Department of Ecology and Evolution, State University of New York, Stony Brook 11794, USA
    Genetics 144:1565-75. 1996
    ..36 million yr old. Although it is widely accepted that inversions are examples of balanced polymorphisms, none of the current neutrality tests including our Monte Carlo simulations showed significant departure from neutral expectations...
  44. ncbi Inversion polymorphisms and nucleotide variability in Drosophila
    P Andolfatto
    Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, EH9 3JT, UK
    Genet Res 77:1-8. 2001
  45. pmc Associations between sperm competition and natural variation in male reproductive genes on the third chromosome of Drosophila melanogaster
    Anthony C Fiumera
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Genetics 176:1245-60. 2007
    ..Our findings provide evidence that pleiotropy and epistasis are important factors in the genetic architecture of male reproductive success and show that haplotype analyses can identify associations missed in the single-marker approach...
  46. ncbi Molecular evolution of the Est-6 gene in Drosophila melanogaster: contrasting patterns of DNA variability in adjacent functional regions
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, 321 Steinhaus Hall, University of California, Irvine 92697 2525, USA
    Gene 288:167-77. 2002
    We have investigated nucleotide polymorphism at the esterase 6 gene (Est-6) gene, including the complete coding region (1686 bp), as well as the 5'-flanking (1183 bp) and 3'-flanking (193 bp) regions of the gene, in 30 strains of ..
  47. ncbi Genetic polymorphism at two linked loci, Sod and Est-6, in Drosophila melanogaster
    Francisco J Ayala
    Department of Ecology and Evolutionary Biology, University of California, 321 Steinhaus Hall, Irvine, CA 92697 2525, USA
    Gene 300:19-29. 2002
    We have examined the patterns of polymorphism at two linked loci, Sod and Est-6, separated by nearly 1000 kb on the left arm of chromosome 3 of Drosophila melanogaster...
  48. ncbi Variability on the dot chromosome in the Drosophila simulans clade
    Mohamed A F Noor
    Department of Biological Sciences, Life Sciences Bldg, Louisiana State University, Baton Rouge, LA 70803, USA
    Genetica 118:51-8. 2003
    ..Overall, none of the data analyzed were consistent with recent introgression exclusively between D. simulans and D. mauritiana...
  49. pmc Studies of esterase-6 in Drosophila melanogaster. II. The genetics and frequency distributions of naturally occurring variants studied by electrophoretic and heat stability criteria
    B J Cochrane
    Genetics 93:461-78. 1979
    ..of stability variants is consistent with the hypothesis that this variation is due to allelic variation at the Est-6 locus...
  50. pmc Bayesian variable selection for detecting adaptive genomic differences among populations
    Andrea Riebler
    Institute of Social and Preventive Medicine, University of Zurich, Zurich, Switzerland
    Genetics 178:1817-29. 2008
    ..For data sets with small sample sizes, high mutation rates, and/or long sequences, however, methods based on nucleotide statistics should be preferred...
  51. doi Polymorphic Y chromosomes harbor cryptic variation with manifold functional consequences
    Bernardo Lemos
    Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 319:91-3. 2008
    ..Functionally, they affect microtubule stability, lipid and mitochondrial metabolism, and the thermal sensitivity of spermatogenesis. Our findings provide a mechanism for adaptive phenotypic variation associated with the Y chromosome...
  52. pmc Estimating the contribution of mutation, recombination and gene conversion in the generation of haplotypic diversity
    Peter L Morrell
    Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697, USA
    Genetics 173:1705-23. 2006
    ..simulans, and Zea mays. In total, 13 of 27 loci show evidence of gene conversion. For these loci, gene conversion is estimated to contribute an average of twice as much as crossing over to total recombination...
  53. ncbi Latitudinal clines for nucleotide polymorphisms in the Esterase 6 gene of Drosophila melanogaster
    Christopher W Coppin
    CSIRO Entomology, GPO Box 1700, Canberra, ACT, Australia
    Genetica 129:259-71. 2007
    Previous studies have found non-neutral patterns of nucleotide polymorphism in the promoter and coding regions of Est6 in D. melanogaster...
  54. ncbi Estimating effects of a single gene and polygenes on quantitative traits from a diallel design
    Xiang Yang Lou
    Department of Psychiatric Medicine, University of Virginia, 1670 Discovery Dr, Suite 110, Charlottesville, VA 22911 5844, USA
    Genetica 128:471-84. 2006
    ..The computer program for the methods suggested in this article is freely available from the authors...
  55. pmc A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. IV. Nested analyses with cladogram uncertainty and recombination
    A R Templeton
    Department of Biology, Washington University, St Louis, Missouri 63130
    Genetics 134:659-69. 1993
    ..In particular, recombination can be used to assign a physical localization to a given subregion for mutations responsible for significant phenotypic effects...
  56. pmc A genetic analysis of the rose-gespleten region (68C8-69B5) of Drosophila melanogaster
    A M Hoogwerf
    Biochemistry Department, Oxford University, England
    Genetics 118:665-70. 1988
    ..None was rescued by the active Lsp-2 gene transformed on to chromosome II and we conclude that, as yet, we have no lethal mutations of Lsp-2...
  57. ncbi Studies of a homologous gene-enzyme system, esterase C, in Drosophila melanogaster and Drosophila simulans
    C D Triantaphyllidis
    Biochem Genet 8:383-90. 1973
  58. ncbi Genetics of esterases in Drosophila. III. Influence of different chromosomes on esterase pattern in Drosophila
    L I Korochkin
    Biochem Genet 12:9-24. 1974
  59. ncbi [Development of sexual dimorphism as an expression of gene Est-6 in the ontogenesis of Drosophila melanogaster and Drosophila simulans]
    A A Aronshtam
    Zh Obshch Biol 35:926-33. 1974
  60. pmc Molecular cloning and characterization of esterase-6, a serine hydrolase of Drosophila
    J G Oakeshott
    Proc Natl Acad Sci U S A 84:3359-63. 1987
    The Est-6 gene of Drosophila melanogaster was cloned by screening libraries with synthetic oligonucleotides corresponding to tryptic peptides from purified esterase-6 (Est-6) protein...
  61. ncbi [Primary structure of the esterase s gene from Drosophila virilis]
    P V Sergeev
    Bioorg Khim 15:839-43. 1989
    ..Conceptual translation of the DNA sequence reveals a protein 549 amino acid residues long, its presumptive active site being highly similar to active sites of the known esterases of insects and mammals...
  62. ncbi Molecular population genetics of structural variants of esterase 6 in Drosophila melanogaster
    J G Oakeshott
    Commonwealth Scientific and Industrial Research Organisation, Division of Entomology, Canberra, Australia
    Genome 31:788-96. 1989
    Several lines of evidence indicate that natural selection operates between the major EST6-F and EST6-S allozymes of Drosophila melanogaster...
  63. ncbi Population genetics of silent and replacement variation in Drosophila simulans and D. melanogaster: X/autosome differences?
    D J Begun
    Mol Biol Evol 13:1405-7. 1996
  64. ncbi The number and distribution of esterase 6 alleles in populations of Drosophila melanogaster
    J Labate
    CSIRO Division of Entomology, Canberra, ACT, Australia
    Heredity (Edinb) 63:203-8. 1989
    High resolution electrophoretic analyses of the polymorphic esterase 6 enzyme have been carried out on 133 isoallelic lines from three Australian populations of Drosophila melanogaster spanning 25 degrees of latitude...
  65. ncbi Causes and consequences of esterase 6 enzyme activity variation in pre-adult Drosophila melanogaster
    J G Oakeshott
    CSIRO Division of Entomology, Canberra, Australia
    Heredity (Edinb) 73:160-9. 1994
    We report heritable threefold differences in both larval and pupal esterase 6 activity among 17 isoallelic lines of D. melanogaster extracted from a natural population...
  66. pmc Decline in heterozygosity under full-sib and double first-cousin inbreeding in Drosophila melanogaster
    W Rumball
    CSIRO Division of Animal Production, Blacktown, New South Wales, Australia
    Genetics 136:1039-49. 1994
    ..Natural selection was shown to favor heterozygotes in the inbred lines. Associative overdominance was the most probable explanation for the slower than expected decline in heterozygosity...
  67. ncbi Geographical differentiation of allozymic variability in natural Indian populations of Drosophila melanogaster
    R Parkash
    Department of Biosciences, Maharshi Dayanand University, Rohtak, India
    Biochem Genet 32:63-73. 1994
    ..The results add support to the hypothesis that the occurrence of parallel or complementary latitudinal clines across different continental populations provides evidence of natural selection maintaining such clinal variation...
  68. ncbi Conservation and change in structural and 5' flanking sequences of esterase 6 in sibling Drosophila species
    J Karotam
    Genetica 90:79-80. 1993
  69. ncbi [Esterase-6 gene-enzyme system and resistance of Drosophila to increased temperature]
    V N Totskiĭ
    Genetika 30:342-8. 1994
    The effect of the gene-enzyme system of esterase-6 (EST-6) on the expression of viability traits in Drosophila melanogaster was studied at normal and high temperatures...
  70. ncbi [Primary structure of the transcription initiation region of the esterase S gene of Drosophila virilis]
    G N Enikolopov
    Bioorg Khim 14:1671-7. 1988
    ..The transcription initiation region is sequenced. The region contains various motifs characteristic for eukaryotic promoters...
  71. ncbi Serine esterases: structural conservation during animal evolution and variability in enzymatic properties in the genus Drosophila
    J Pen
    Department of Medical Microbiology, Rijksuniversiteit Groningen, The Netherlands
    Genetica 81:125-31. 1990
    ..This difference in substrate preference is another example of the variability observed with Drosophila esterases...
  72. pmc Empirical tests of some predictions from coalescent theory with applications to intraspecific phylogeny reconstruction
    K A Crandall
    Department of Biology, Washington University, St Louis, Missouri 63130 4899
    Genetics 134:959-69. 1993
    ....
  73. ncbi [Mutational variability of enzyme loci in unstable Drosophila melanogaster strains]
    N I Ivashchenko
    Genetika 26:257-65. 1990
    ..Activity and mobility variants of alpha-GPDH, ADH, SOD, G6PD, 6PGD and EST-6 were analysed...
  74. ncbi Inference of haplotypes from PCR-amplified samples of diploid populations
    A G Clark
    Department of Biology, Pennsylvania State University, University Park 16802
    Mol Biol Evol 7:111-22. 1990
    ..The algorithm also applies to the problem of inferring haplotype frequencies of closely linked restriction-site polymorphisms...
  75. pmc Associations between restriction site polymorphism and enzyme activity variation for esterase 6 in Drosophila melanogaster
    A Y Game
    C S I R O, Division of Entomology, Canberra City, ACT, Australia
    Genetics 126:1021-31. 1990
    Thirty-five nucleotide polymorphisms were found in a 21.5-kbp region including the Est6 locus among 42 isoallelic lines extracted from a single natural population of Drosophila melanogaster...
  76. pmc Analysis of CpG suppression in methylated and nonmethylated species
    D F Schorderet
    Department of Medicine, University of Washington, Seattle 98195
    Proc Natl Acad Sci U S A 89:957-61. 1992
    ..We speculate that the excess of CpGs at position III-I in nonmethylated forms may be related to a requirement for minimal thermal stability of the DNA...
  77. ncbi Localization of sequences regulating ancestral and acquired sites of esterase6 activity in Drosophila melanogaster
    M J Healy
    CSIRO Division of Entomology, Canberra, Australia
    Mol Biol Evol 13:784-97. 1996
    We have broadly defined the DNA regions regulating esterase6 activity in several life stages and tissue types of D...
  78. ncbi Mutational analysis of N-linked glycosylation of esterase 6 in Drosophila melanogaster
    M A Myers
    CSIRO Division of Entomology, Canberra, Australia
    Biochem Genet 34:201-18. 1996
    The primary sequence of the esterase 6 (EST6) enzyme of Drosophila melanogaster contains four potential N-linked glycosylation sites, at residues 21, 399, 435, and 485...
  79. ncbi Drosophila: the genetics of two major larval proteins
    M E Akam
    Cell 13:215-25. 1978
    ..The structural genes for esterase 6 and four visible recessive loci lie within the same region.
  80. ncbi The developments between gametogenesis and fertilization: ovulation and female sperm storage in Drosophila melanogaster
    Margaret C Bloch Qazi
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Dev Biol 256:195-211. 2003
    ..melanogaster, both processes are triggered by male factors during and after mating, including sperm and seminal fluid proteins. Therefore, an interplay of male and female factors coordinates the gametes for fertilization...
  81. pmc Amino acid polymorphisms for esterase-6 in Drosophila melanogaster
    P H Cooke
    Research School of Biological Sciences, Australian National University, Canberra
    Proc Natl Acad Sci U S A 86:1426-30. 1989
    ..1.1.1) in Drosophila melanogaster. The sequences of 13 isolates of the Est6 gene covering all 10 allozymes were obtained and 52 nucleotide differences were found...
  82. ncbi Studies of esterase 6 in Drosophila melanogaster. XIII. Purification and characterization of the two major isozymes
    S D Mane
    Biochem Genet 21:1019-40. 1983
    Esterase-6 (EST 6; carboxylic-ester hydrolase; EC 3.1.1.1) from Drosophila melanogaster was purified to homogenity...
  83. pmc DNA polymorphism in the beta-Esterase gene cluster of Drosophila melanogaster
    Evgeniy S Balakirev
    Department of Ecology and Evolutionary Biology, University of California, Irvine 92697 2525, USA
    Genetics 164:533-44. 2003
    We have analyzed nucleotide polymorphism within a 5.3-kb region encompassing the functional Est-6 gene and the psiEst-6 putative pseudogene in 28 strains of Drosophila melanogaster and one of D. simulans...
  84. ncbi The mutagenicity of Gramoxone (paraquat) on different eukaryotic systems
    A Z el-Abidin Salam
    Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
    Mutat Res 319:89-101. 1993
    ..At the protein level, Gramoxone had detectable mutagenic effects on the genetic background of two enzymes, Adh and Est-6. Gramoxone should be considered a mutagenic herbicide.
  85. pmc Specificity and localization of lipolytic activity in adult Drosophila melanogaster
    G M Smith
    Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, U K
    Biochem J 304:775-9. 1994
    ..was used to confirm that the enzyme activity was due to a distinct lipase and not non-specific activity from esterase 6 which is also abundant in accessory glands...
  86. ncbi [Geographic variability of Adh, alpha-Gpdh, and Est-6 loci in natural Eurasia Drosophila melanogaster populations]
    O A Bubliĭ
    Genetika 30:467-77. 1994
    The allele frequencies of three allozyme loci, ADH, alpha-GPDH, and EST-6, were assessed in 18 natural populations of Drosophila melanogaster of Eastern Europe, the Caucasus, and Central Asia...
  87. ncbi [Esterase-6 activity in Drosophila melanogaster strains with varying male mating activity]
    D V Korotkov
    Genetika 29:1429-39. 1993
    ..X-chromosomes and chromosome 3 participate in genetic control of interstock differences of both sexual and esterase-6 activity...
  88. ncbi Sexual isolation of genetically differentiated sympatric populations of Drosophila melanogaster in Brazzaville, Congo: the first step towards speciation?
    P Capy
    Laboratoire Populations, Genetique et Evolution, CNRS, 91198 Gif Yvette cedex, France
    Heredity (Edinb) 84:468-75. 2000
    ....
  89. ncbi On the detection of nonrandom associations between DNA polymorphisms in natural populations of Drosophila
    C Zapata
    Museum of Comparative Zoology, Harvard University
    Mol Biol Evol 10:823-41. 1993
    ..variants was examined in eight gene regions of Drosophila melanogaster (yellow-achaetescute, white, Zw, Adh, Est6, and rosy) and D. pseudoobscura (Adh and Xdh), on the basis of published population data...
  90. ncbi The acid phosphatase-1 gene region in the Drosophila species of the subobscura cluster
    A Navarro-Sabate
    Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, Spain
    Hereditas 130:65-75. 1999
    ..madeirensis and D. subobscura. These phylogenetic relationships are, however, not supported by divergence at nonsynonymous sites since the lowest Ka estimate is between D. guanche and D. subobscura...
  91. ncbi Regulatory aspects of esterase 6 activity variation in sibling Drosophila species
    J Karotam
    CSIRO Division of Entomology, Canberra, Australia
    Heredity (Edinb) 71:41-50. 1993
    b>Esterase 6 in Drosophila melanogaster, Drosophila simulans and Drosophila mauritiana is produced in several life stages and diverse tissues, but the major pulse of expression is in the sperm ejaculatory duct of adult males...
  92. pmc Nucleotide polymorphism in the 5' promoter region of esterase 6 in Drosophila melanogaster and its relationship to enzyme activity variation
    W A Odgers
    CSIRO Division of Entomology, Canberra, Australia
    Genetics 141:215-22. 1995
    A 974-bp region immediately 5' of the esterase 6 gene was sequenced in 17 field derived third chromosome isoallelic lines...
  93. ncbi High nucleotide sequence variation in a region of low recombination in Drosophila simulans is consistent with the background selection model
    M T Hamblin
    Section of Genetics and Development, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Evol 13:1133-40. 1996
    ..melanogaster. Alternatively, the deleterious mutation rate may be smaller in D. simulans, or balancing selection may be acting nearby, thereby reducing the effect of background selection...

Research Grants1

  1. Comparative Genomics of Seminal Fluid Proteins
    ANTHONY FIUMERA; Fiscal Year: 2005
    ..Finally, I will then determine the functional conservation of Acps using transgenic D. melanogaster expressing heterospecific accessory gland proteins. ..