Eip75B

Summary

Gene Symbol: Eip75B
Description: Ecdysone-induced protein 75B
Alias: 57B, 75B, BcDNA:GM02640, CG8127, DmE75A, DmE75B, Dmel\CG8127, E75, E75-C, E75A, E75B, E75C, EP1121b, Eip75, Eip75 A, Eip75A, Mgn00274, NR1D3, Rev-Erb, anon-WO0172774.31, anon-WO0172774.32, dE75, eip75B, l(3)07041, l(3)j11A6, l(3)j12E8, l(3)j3A6, l(3)j5E1, l(3)neo25, CG8127 gene product from transcript CG8127-RF, CG8127-PA, CG8127-PB, CG8127-PC, CG8127-PD, CG8127-PE, CG8127-PF, CG8127-PG, E75 nuclear receptor, Eip75B-PA, Eip75B-PB, Eip75B-PC, Eip75B-PD, Eip75B-PE, Eip75B-PF, Eip75B-PG, ecdysone induced protein 75, ecdysone inducible protein 75B, ecdysone-induced protein 75
Species: fruit fly

Top Publications

  1. pmc Temporal coordination of regulatory gene expression by the steroid hormone ecdysone
    F D Karim
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112
    EMBO J 11:4083-93. 1992
  2. ncbi Hormones, puffs and flies: the molecular control of metamorphosis by ecdysone
    A J Andres
    Howard Hughes Medical Institute, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112
    Trends Genet 8:132-8. 1992
  3. ncbi The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily
    M R Koelle
    Department of Biochemistry, Stanford University Medical Center, California 94305
    Cell 67:59-77. 1991
  4. ncbi The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily
    W A Segraves
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    Genes Dev 4:204-19. 1990
  5. ncbi Sequential gene activation by ecdysone in Drosophila melanogaster: the hierarchical equivalence of early and early late genes
    F Huet
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, CU de Strasbourg, France
    Development 121:1195-204. 1995
  6. ncbi The Drosophila Broad-Complex plays a key role in controlling ecdysone-regulated gene expression at the onset of metamorphosis
    F D Karim
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112
    Development 118:977-88. 1993
  7. ncbi Puffs and PCR: the in vivo dynamics of early gene expression during ecdysone responses in Drosophila
    F Huet
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, Unite 184 de Biologie Moleculaire et de Genie Genetique de l INSERM, Faculte de Medecine, Strasbourg, France
    Development 118:613-27. 1993
  8. ncbi The reaction with polytene chromosomes of antibodies raised against Drosophila E75A protein
    R J Hill
    CSIRO Division of Biomolecular Engineering, Laboratory for Molecular Biology, North Ryde, NSW, Australia
    Insect Biochem Mol Biol 23:99-104. 1993
  9. ncbi Flies on steroids--Drosophila metamorphosis and the mechanisms of steroid hormone action
    C S Thummel
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    Trends Genet 12:306-10. 1996
  10. pmc Ligand binding was acquired during evolution of nuclear receptors
    H Escriva
    Institut National de la Santé et de la Recherche Médicale U167 Relations Hôte Parasite et Stratégies Vaccinales, Institut Pasteur de Lille, 1 rue Calmette, 59019, Lille Cedex, France
    Proc Natl Acad Sci U S A 94:6803-8. 1997

Research Grants

Scientific Experts

Detail Information

Publications108 found, 100 shown here

  1. pmc Temporal coordination of regulatory gene expression by the steroid hormone ecdysone
    F D Karim
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112
    EMBO J 11:4083-93. 1992
    ..concentration peaks in late third instar larvae, these transcripts are repressed and the class II RNAs (E74A, E75A and E75B) are induced. The BR-C RNAs appear to have both class I and class II characteristics...
  2. ncbi Hormones, puffs and flies: the molecular control of metamorphosis by ecdysone
    A J Andres
    Howard Hughes Medical Institute, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112
    Trends Genet 8:132-8. 1992
    ..Molecular characterization of some of these genes has provided valuable clues to regulatory mechanisms by which the ecdysone signal is transduced and amplified...
  3. ncbi The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily
    M R Koelle
    Department of Biochemistry, Stanford University Medical Center, California 94305
    Cell 67:59-77. 1991
    ..As expected, EcR is nuclear and found in all ecdysone target tissues examined. Furthermore, the EcR gene is expressed at each developmental stage marked by a pulse of ecdysone...
  4. ncbi The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily
    W A Segraves
    Department of Biochemistry, Stanford University School of Medicine, California 94305 5307
    Genes Dev 4:204-19. 1990
    ..We isolated a 50-kb ecdysone-inducible gene, E75, that occupies the early puff locus at 75B. E75 contains two overlapping transcription units...
  5. ncbi Sequential gene activation by ecdysone in Drosophila melanogaster: the hierarchical equivalence of early and early late genes
    F Huet
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, CU de Strasbourg, France
    Development 121:1195-204. 1995
    ..by a precise sequential activation of members of the superfamily of nuclear receptors (DHR3, DHR39, EcR, E75, E78, FTZ-F1, usp)...
  6. ncbi The Drosophila Broad-Complex plays a key role in controlling ecdysone-regulated gene expression at the onset of metamorphosis
    F D Karim
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112
    Development 118:977-88. 1993
    ..The l(1)2Bc function is also required for the complete ecdysone induction of some early mRNAs (E74A, E75A, and BR-C) and efficient repression of most early mRNAs in prepupae...
  7. ncbi Puffs and PCR: the in vivo dynamics of early gene expression during ecdysone responses in Drosophila
    F Huet
    Laboratoire de Genetique Moleculaire des Eucaryotes du CNRS, Unite 184 de Biologie Moleculaire et de Genie Genetique de l INSERM, Faculte de Medecine, Strasbourg, France
    Development 118:613-27. 1993
    ..A number of these early puffs (2B5, 74EF and 75B) contain complex transcription units (Broad-Complex, E74 and E75 respectively) encoding families of regulatory ..
  8. ncbi The reaction with polytene chromosomes of antibodies raised against Drosophila E75A protein
    R J Hill
    CSIRO Division of Biomolecular Engineering, Laboratory for Molecular Biology, North Ryde, NSW, Australia
    Insect Biochem Mol Biol 23:99-104. 1993
    ..The Drosophila E75 early gene has been isolated and two of its products, E75A and E75B, have been shown to be members of the steroid receptor superfamily...
  9. ncbi Flies on steroids--Drosophila metamorphosis and the mechanisms of steroid hormone action
    C S Thummel
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    Trends Genet 12:306-10. 1996
    ..It seems likely that similar hormone-triggered regulatory hierarchies exist in other higher organisms and that Drosophila is providing our first glimpses of the complexities of these gene networks...
  10. pmc Ligand binding was acquired during evolution of nuclear receptors
    H Escriva
    Institut National de la Santé et de la Recherche Médicale U167 Relations Hôte Parasite et Stratégies Vaccinales, Institut Pasteur de Lille, 1 rue Calmette, 59019, Lille Cedex, France
    Proc Natl Acad Sci U S A 94:6803-8. 1997
    ..Strikingly, our results suggest that the ancestral NR was an orphan receptor that acquired ligand-binding ability during subsequent evolution...
  11. ncbi Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms
    M Bender
    Department of Developmental Biology, Stanford University Medical Center, California 94305, USA
    Cell 91:777-88. 1997
    ..Comparable transgenic expression of EcR-B1, EcR-B2, and EcR-A in these mutant glands results, respectively, in full, partial, and no repair of that loss...
  12. ncbi The RXR homolog ultraspiracle is an essential component of the Drosophila ecdysone receptor
    B L Hall
    Howard Hughes Medical Institute, University of Utah, Salt Lake City, UT 84112 5331, USA
    Development 125:4709-17. 1998
    ....
  13. ncbi Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila
    M Buszczak
    Department of MCDB, Yale University, New Haven, CT 06520 8103, USA
    Development 126:4581-9. 1999
    ..b>E75, E74 and BR-C are expressed in a stage-specific manner while EcR expression is ubiquitous throughout oogenesis...
  14. ncbi The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids
    M Schubiger
    Department of Zoology, University of Washington, Seattle WA 98195, USA
    Development 127:1151-9. 2000
    ..the wing disc of Drosophila shows that in the absence of USP, early hormone responsive genes such as EcR, DHR3 and E75B fail to up-regulate in response to 20E, but other genes that are normally expressed later, such as (&bgr;)-Ftz-..
  15. ncbi A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila
    T Li
    Department of Genetics, The University of Georgia, Athens, GA 30602 7223, USA
    Development 127:2897-905. 2000
    ..These results show that EcR mediates ecdysone signaling at multiple developmental stages and implicate EcR in the reorganization of imaginal and larval tissues at the onset of metamorphosis...
  16. ncbi A steroid-triggered transcriptional hierarchy controls salivary gland cell death during Drosophila metamorphosis
    C Jiang
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah, Salt Lake City 84112, USA
    Mol Cell 5:445-55. 2000
    ..diap2 induction is dependent on betaFTZ-F1, while E75A and E75B are each sufficient to repress diap2...
  17. ncbi Steroid regulation of postembryonic development and reproduction in Drosophila
    T Kozlova
    Howard Hughes Medical Institute, 15 N 2030 E Rm 5100, University of Utah, UT 84112 5331, USA
    Trends Endocrinol Metab 11:276-80. 2000
    ..The ecdysteroid hierarchy was also shown recently to regulate Drosophila oogenesis and reproduction...
  18. ncbi E93 directs steroid-triggered programmed cell death in Drosophila
    C Y Lee
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park 20742, USA
    Mol Cell 6:433-43. 2000
    ..Furthermore, expression of E93 is sufficient to induce programmed cell death. We propose that the steroid induction of E93 determines a programmed cell death response during development...
  19. ncbi Insect metamorphosis: out with the old, in with the new
    M Buszczak
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520 8103, USA
    Curr Biol 10:R830-3. 2000
    ..Recent studies suggest that the E93 gene is both necessary and sufficient to target tissues for ecdysone-induced apoptosis...
  20. ncbi Glue secretion in the Drosophila salivary gland: a model for steroid-regulated exocytosis
    A Biyasheva
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Chicago, Illinois 60611 3093, USA
    Dev Biol 231:234-51. 2001
    ..The results presented establish a connection between 20E exposure and changes in Ca2+ levels that are mediated by Ca2+ effector proteins, and thus establish a mechanistic framework for future studies...
  21. ncbi Loss of the ecdysteroid-inducible E75A orphan nuclear receptor uncouples molting from metamorphosis in Drosophila
    Michael Bialecki
    Howard Hughes Medical Institute, Department of Human Genetics, 15 North 2030 East, University of Utah, Salt Lake City 84112, USA
    Dev Cell 3:209-20. 2002
    ..were used to define the functions of three orphan members of the nuclear receptor superfamily, E75A, E75B, and E75C, encoded by the E75 early ecdysteroid-inducible gene...
  22. ncbi rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development
    Julie Gates
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112 5331, USA
    Development 131:25-36. 2004
    ..duplicated mouth parts, and defects in puparium formation--phenotypes that resemble those seen in EcR, usp, E75A and betaFTZ-F1 mutants...
  23. ncbi Hormonal regulation and functional role of Drosophila E75A orphan nuclear receptor in the juvenile hormone signaling pathway
    Edward B Dubrovsky
    Department of Biology, Dartmouth College, Hanover, NH 03755, USA
    Dev Biol 268:258-70. 2004
    ..How these two hormones interact at the molecular level is not known. Here we report the JH inducibility of the E75A nuclear receptor encoded by the E75 early ecdysone-inducible gene...
  24. ncbi Nuclear receptors--a perspective from Drosophila
    Kirst King-Jones
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, 15 North 2030 East, Room 5100, Salt Lake City, Utah 84112 5331, USA
    Nat Rev Genet 6:311-23. 2005
    ..Here, we review recent breakthroughs in our understanding of D. melanogaster nuclear receptors, and interpret these results in light of findings from their evolutionarily conserved vertebrate homologues...
  25. ncbi The Drosophila nuclear receptor e75 contains heme and is gas responsive
    Jeff Reinking
    Banting and Best Department of Medical Research, Charles H Best Institute, 112 College Street, Toronto, Ontario, M5G 1L6, Canada
    Cell 122:195-207. 2005
    ..Here, we demonstrate that the ligand binding pocket of the Drosophila nuclear receptor E75 contains a heme prosthetic group...
  26. pmc Dynamic regulation of Drosophila nuclear receptor activity in vivo
    Laura Palanker
    Department of Human Genetics, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City, UT 84112 5331, USA
    Development 133:3549-62. 2006
    ....
  27. pmc The Drosophila histone acetyltransferase Gcn5 and transcriptional adaptor Ada2a are involved in nucleosomal histone H4 acetylation
    Anita Ciurciu
    Institute of Biochemistry, Biological Research Center, Temesvári krt, H 6726 Szeged, Hungary
    Mol Cell Biol 26:9413-23. 2006
    ..Our data suggest dAda2a involvement in both uniformly distributed H4 acetylation and gene-specific transcription regulation...
  28. pmc dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis
    Arash Bashirullah
    Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112 5330, USA
    Dev Dyn 236:3173-9. 2007
    ..The transcription of key ecdysone-regulated target genes is delayed and reduced in dTrf2 mutants. dTrf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis...
  29. ncbi Hormonal regulation of the E75 gene in Drosophila: identifying functional regulatory elements through computational and biological analysis
    Travis J Bernardo
    Department of Biology, Fordham University, Bronx, NY 10458, USA
    J Mol Biol 387:794-808. 2009
    ..E75 occupies over 100 kb of genomic DNA; it has four alternative promoters producing isoforms E75A, E75B, E75C, and E75D...
  30. doi dDOR is an EcR coactivator that forms a feed-forward loop connecting insulin and ecdysone signaling
    Víctor A Francis
    German Cancer Research Center DKFZ, Im Neuenheimer Feld 580, Heidelberg, Germany
    Curr Biol 20:1799-808. 2010
    ..Because no DOR loss-of-function mammalian models are available, we analyze here the in vivo function of DOR by studying flies mutant for Drosophila DOR (dDOR)...
  31. pmc The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila
    Elizabeth T Ables
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA
    Cell Stem Cell 7:581-92. 2010
    ..Our findings support a link between systemic steroid hormones and the intrinsic chromatin remodeling machinery as a potential mechanism to promote broad transcriptional programs required for adult stem cell self-renewal...
  32. pmc Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75
    Lucía Cáceres
    Banting and Best Department of Medical Research, Department of Molecular Biology, University of Toronto, Ontario, Canada
    Genes Dev 25:1476-85. 2011
    ..produced in the Drosophila prothoracic gland, where it acts via the nuclear receptor ecdysone-induced protein 75 (E75), reversing its ability to interfere with its heterodimer partner, Drosophila hormone receptor 3 (DHR3)...
  33. pmc The Drosophila FTZ-F1 nuclear receptor mediates juvenile hormone activation of E75A gene expression through an intracellular pathway
    Edward B Dubrovsky
    Department of Biology, Fordham University, Bronx, New York 10458, USA
    J Biol Chem 286:33689-700. 2011
    ..We previously found that the nuclear receptor gene E75A is activated in S2 cells as a primary response to JH...
  34. pmc Ecdysone- and NO-mediated gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development
    Danika M Johnston
    Department of Biochemistry and Molecular Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Mol Cell 44:51-61. 2011
    ..of ecdysone, both ecdysone receptor subunits localize to the cytoplasm, and the heme-binding nuclear receptor E75A replaces EcR/Usp at common target sequences in several genes...
  35. doi Insect nuclear receptors
    Susan E Fahrbach
    Department of Biology, Wake Forest University, Winston Salem, North Carolina 27109, USA
    Annu Rev Entomol 57:83-106. 2012
    ..An exciting new technology allows EcR to be used in chimeric, ligand-inducible gene-switch systems with applications in pest management and medicine...
  36. doi Secreted peptide Dilp8 coordinates Drosophila tissue growth with developmental timing
    Julien Colombani
    Universite de Nice, INSERM 1091, CNRS 7277, and France Institute of Biology, Parc Valrose, 06108 Nice, France
    Science 336:582-5. 2012
    ..We propose that Dilp8 peptide is a secreted signal that coordinates the growth status of tissues with developmental timing...
  37. pmc Histone recognition and nuclear receptor co-activator functions of Drosophila cara mitad, a homolog of the N-terminal portion of mammalian MLL2 and MLL3
    Chhavi Chauhan
    Oncology Institute, Stritch School of Medicine, Loyola University of Chicago, Maywood, IL 60153, USA
    Development 139:1997-2008. 2012
    ..The evolutionary split of MLL2 into two distinct genes in Drosophila provides important insight into distinct epigenetic functions of conserved readers and writers of the histone code...
  38. pmc Covalent attachment of heme to the protein moiety in an insect E75 nitric oxide sensor
    Clara Aicart-Ramos
    Departamento de Bioquimica y Biologia Molecular I, Universidad Complutense, 28040 Madrid, Spain
    Biochemistry 51:7403-16. 2012
    ..have recombinantly expressed and purified the ligand binding domains (LBDs) of four insect nuclear receptors of the E75 family...
  39. pmc Evolution of the nuclear receptor gene superfamily
    V Laudet
    INSERM U186 CNRS URA 1160, Institut Pasteur, Lille, France
    EMBO J 11:1003-13. 1992
    ..These data suggest a complex evolutionary history for nuclear receptor genes in which gene duplication events and swapping between domains of different origins took place...
  40. ncbi Something old, some things new: the steroid receptor superfamily in Drosophila
    W A Segraves
    Howard Hughes Medical Institute, Gene Expression Lab, Salk Institute, La Jolla, California 92037
    Cell 67:225-8. 1991
  41. ncbi Advantages of a P-element construct containing MtnA sequences for the identification of patterning and cell determination genes in Drosophila melanogaster
    M Faucheux
    Laboratoire d Embryologie Moleculaire et Experimentale, CNRS UPRES A 8080, Universite Paris XI, Orsay, France
    Mol Genet Genomics 265:14-22. 2001
    ..Taken together, these results indicate that P[MTW] allows the tagging of patterning and cell determination genes, and thus provides a useful tool for identifying new developmental functions...
  42. pmc Comparison of complete nuclear receptor sets from the human, Caenorhabditis elegans and Drosophila genomes
    J M Maglich
    Nuclear Receptor Discovery Research, GlaxoSmithKline, Research Triangle Park, NC 27709, USA
    Genome Biol 2:RESEARCH0029. 2001
    ..This information was used to carry out a comparative genomic study of the NR superfamily...
  43. ncbi Juvenile hormone analogs do not affect directly the activity of the ecdysteroid receptor complex in insect culture cell lines
    Thomas Soin
    Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium
    J Insect Physiol 54:429-38. 2008
    ..the ecdysteroid receptor transcription complex and (2) interference at the level of induction of the primary gene E75. Our data do not support JH analogs (JHAs) acting through the above processes: 'antagonism' of ecdysteroid receptor ..
  44. doi Overexpression of HmgD causes the failure of pupariation in Drosophila by affecting ecdysone receptor pathway
    Jing Chen
    Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, PR China
    Arch Insect Biochem Physiol 68:123-33. 2008
    ..of ecdysone receptor gene EcR, whereas transcriptional patterns of some key regulatory genes, such as E74A, E74B, E75A, E75B, betaFTZ-F1, are changed greatly...
  45. doi DHR51, the Drosophila melanogaster homologue of the human photoreceptor cell-specific nuclear receptor, is a thiolate heme-binding protein
    Eve de Rosny
    CEA, CNRS, Universite Joseph Fourier, UMR 5075, Institut de Biologie Structurale Jean Pierre Ebel, 38027 Grenoble Cedex 1, France
    Biochemistry 47:13252-60. 2008
    ..for the activity of the human nuclear receptors (NR) REV-ERBalpha and REV-ERBbeta and their Drosophila homologue E75. Here, we report the cloning, expression in Escherichia coli, purification, and screening for the heme-binding ..
  46. pmc Genetic screen for regulators of lymph gland homeostasis and hemocyte maturation in Drosophila
    Kai Li Tan
    Waksman Institute, Rutgers University, Piscataway, New Jersey 08854
    G3 (Bethesda) 2:393-405. 2012
    ....
  47. pmc Spatial profiling of nuclear receptor transcription patterns over the course of Drosophila development
    Ronit Wilk
    Department of Molecular Genetics, The Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada
    G3 (Bethesda) 3:1177-89. 2013
    ..These patterns will serve as a useful reference for future studies on the tissue-specific roles and interactions of nuclear receptor proteins, partners, cofactors and ligands. ..
  48. pmc Genetic control of specificity to steroid-triggered responses in Drosophila
    Robert J Ihry
    Division of Pharmaceutical Sciences, University of Wisconsin, Madison, Wisconsin 53705
    Genetics 196:767-80. 2014
    ....
  49. pmc PPAR gamma activation is neuroprotective in a Drosophila model of ALS based on TDP-43
    Archi Joardar
    Department of Molecular and Cellular Biology
    Hum Mol Genet 24:1741-54. 2015
    ..Using a pharmacogenetic approach, we show that the predicted Drosophila PPARγ homologs, E75 and E78, are in vivo targets of pioglitazone...
  50. pmc Transcriptional regulation via nuclear receptor crosstalk required for the Drosophila circadian clock
    Edouard Jaumouillé
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland Department of Genetics and Evolution, Sciences III, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    Curr Biol 25:1502-8. 2015
    ..We show here that the nuclear receptor E75, the fly homolog of REV-ERB α and REV-ERB β, and the NR2E3 subfamily nuclear receptor UNF are components of the ..
  51. doi Ecdysone and insulin signaling play essential roles in readjusting the altered body size caused by the dGPAT4 mutation in Drosophila
    Yan Yan
    State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100080, China
    J Genet Genomics 42:487-94. 2015
    ..Thus, we propose that a strategy has been evolved by the animals to reach final body size when challenged by genetic alterations, which requires the coordinated ecdysone and insulin signaling. ..
  52. pmc The Genetic Architecture of Natural Variation in Recombination Rate in Drosophila melanogaster
    Chad M Hunter
    Program in Genetics, Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, United States of America
    PLoS Genet 12:e1005951. 2016
    ..Our results highlight the magnitude of population variation in recombination rate present in D. melanogaster and implicate new genetic factors mediating natural variation in this quantitative trait. ..
  53. pmc The Drosophila nuclear receptors DHR3 and betaFTZ-F1 control overlapping developmental responses in late embryos
    Anne Françoise Ruaud
    Department of Human Genetics, University of Utah School of Medicine, 15 N 2030 E Room 2100, Salt Lake City, UT 84112 5330, USA
    Development 137:123-31. 2010
    ..that consists of the sequential expression of the transcription-factor-encoding genes DHR3, betaFTZ-F1, E74A and E75A. Although the regulatory interactions between these genes have been well characterized by genetic and molecular ..
  54. doi Sox14 is required for transcriptional and developmental responses to 20-hydroxyecdysone at the onset of drosophila metamorphosis
    Amanda R Ritter
    Department of Poultry Science, The University of Georgia, Athens, Georgia 30602, USA
    Dev Dyn 239:2685-94. 2010
    ..We also show that the Sox14-regulated gene set correlates well with Sox14 expression in a variety of larval and adult tissues. Thus, Sox14 is a critical transcription factor required for 20E signaling at the onset of metamorphosis...
  55. pmc Chromatin landscape dictates HSF binding to target DNA elements
    Michael J Guertin
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
    PLoS Genet 6:e1001114. 2010
    ..There is a strong correlation of bound HSEs to active chromatin marks present prior to induced HSF binding, indicating that an HSE's residence in "active" chromatin is a primary determinant of whether HSF can bind following heat shock...
  56. pmc Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function
    Geetanjali Chawla
    Jordan Hall A502, Department of Biology, Indiana University, Bloomington, IN 47405, USA
    Development 139:1788-97. 2012
    ....
  57. ncbi PKC-mediated USP phosphorylation is required for 20E-induced gene expression in the salivary glands of Drosophila melanogaster
    Xiaoping Sun
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Arch Insect Biochem Physiol 62:116-27. 2006
    ..The composite data suggest that PKC-mediated USP phosphorylation is required for 20E-induced gene expression in the salivary glands of Drosophila melanogaster...
  58. doi Ultrafast heme-residue bond formation in six-coordinate heme proteins: implications for functional ligand exchange
    Marten H Vos
    Laboratoire d Optique et Biosciences, CNRS, Ecole Polytechnique, F 91128 Palaiseau, France
    Biochemistry 47:5718-23. 2008
    ..a bacterial heme-binding superoxide dismutase (SOD), plant cytochrome b 559, the insect nuclear receptor E75, horse heart cytochrome c and the heme domain of the bacterial sensor protein Dos...
  59. pmc Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes
    Jan A van der Knaap
    Department of Biochemistry, Erasmus University Medical Center, 3000 DR Rotterdam, The Netherlands
    Mol Cell Biol 30:736-44. 2010
    ..These results revealed the cooperation between a biosynthetic enzyme and a ubiquitin protease in developmental gene control by hormone receptors...
  60. pmc Isoform-specific regulation of a steroid hormone nuclear receptor by an E3 ubiquitin ligase in Drosophila melanogaster
    Ana Citlali Gradilla
    Instituto Cajal, Consejo Superior de Investigaciones Cientificas, Madrid 28002, Spain
    Genetics 189:871-83. 2011
    ..qRT-PCR experiments show that the ARI-1a levels lead to the transcriptional regulation of Eip78C, Eip74EF, Eip75B, and Br-C, as well as that of EcR and usp genes...
  61. pmc Coordinated regulation of niche and stem cell precursors by hormonal signaling
    Dana Gancz
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
    PLoS Biol 9:e1001202. 2011
    ..Similar principles might govern the formation of additional stem cell units during organogenesis...
  62. doi Ecdysone receptor (EcR) suppresses lipid accumulation in the Drosophila fat body via transcription control
    Yuki Kamoshida
    The Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Biochem Biophys Res Commun 421:203-7. 2012
    ..Two lipid metabolism regulatory factor, E75B and adipose (adp) as well as cell growth factor, dMyc, were found as EcR target genes in the adipocyte-like cells, ..
  63. ncbi The Drosophila nuclear receptors: new insight into the actions of nuclear receptors in development
    A E Oro
    Howard Hughes Medical Institute, Gene Expression Lab, La Jolla, California 92186 5800
    Curr Opin Genet Dev 2:269-74. 1992
    ..This suggests that genetic analysis in flies could facilitate the generation of biological models that pertain to complex hormonal responses in development and which are relevant to both vertebrate and invertebrate systems...
  64. ncbi (HX)n repeats: a pH-controlled protein-protein interaction motif of eukaryotic transcription factors?
    R Janknecht
    Institut fur Molekularbiologie, Medizinische Hochschule Hannover, Germany
    FEBS Lett 295:1-2. 1991
    ..g. HPHAHPHP in paired protein. Detailed molecular modelling and database searches lead to the suggestion that (HX)n repeats can mediate interaction between transcription factors in a pH-controlled fashion...
  65. ncbi Puffs and gene regulation--molecular insights into the Drosophila ecdysone regulatory hierarchy
    C S Thummel
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah Medical Center, Salt Lake City 84132
    Bioessays 12:561-8. 1990
    ..Proteins encoded by two of the early genes bind to both early and late ecdysone-induced puffs, implying that they are key regulators in the hierarchy...
  66. pmc A member of the steroid hormone receptor gene family is expressed in the 20-OH-ecdysone inducible puff 75B in Drosophila melanogaster
    G Feigl
    Lehrstuhl für Biochemie, Ruhr Universitat Bochum, FRG
    Nucleic Acids Res 17:7167-78. 1989
    Drosophila melanogaster DNA has been cloned which encompasses a major part of the 20-OH-ecdysone inducible puff 75B. One 20-OH-ecdysone responsive transcription unit was detected which is expressed into two transcripts which accumulate ..
  67. ncbi Steroid hormone receptor homologs in development
    A E Oro
    Howard Hughes Medical Institute, Salk Institute for Biological Sciences, La Jolla, California 92138 9216
    Development 107:133-40. 1989
    ..knrl is one of the most diverged steroid receptor-like molecules and displays a spatially restricted blastoderm pattern...
  68. pmc Isolation, regulation, and DNA-binding properties of three Drosophila nuclear hormone receptor superfamily members
    G J Fisk
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    Proc Natl Acad Sci U S A 92:10604-8. 1995
    ..The 20E receptor can bind to each of the sequences recognized by DHR78 and DHR96, indicating that these proteins may compete with the receptor for binding to a common set of target sequences...
  69. ncbi Insect nuclear receptors: a developmental and comparative perspective
    V C Henrich
    Department of Biology, University of North Carolina Greensboro 27412 5001, USA
    Insect Biochem Mol Biol 25:881-97. 1995
    ..As receptor homologues have been identified in other insects, several general ideas concerning insect hormonal regulation have begun to emerge and these will be examined from a comparative point of view...
  70. ncbi Isolation, characterization and developmental expression of the ecdysteroid-induced E75 gene of the wax moth Galleria mellonella
    M Jindra
    Institute of Entomology, Czech Academy of Sciences, Ceske Budejovice
    Eur J Biochem 221:665-75. 1994
    Using the cDNA for the Drosophila ecdysteroid-induced member of the steroid-hormone-receptor superfamily, E75A, we isolated a genomic clone from Galleria mellonella that revealed 77% similarity with the region of E75A cDNA encoding the C-..
  71. ncbi From embryogenesis to metamorphosis: the regulation and function of Drosophila nuclear receptor superfamily members
    C S Thummel
    Howard Hughes Medical Institute, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112, USA
    Cell 83:871-7. 1995
  72. pmc The ecdysone-inducible Broad-complex and E74 early genes interact to regulate target gene transcription and Drosophila metamorphosis
    J C Fletcher
    Department of Human Genetics, Howard Hughes Medical Institute, Eccles Institute of Human Genetics, University of Utah, Salt Lake City 84112, USA
    Genetics 141:1025-35. 1995
    ..This data is consistent with current models which propose that combinations of ecdysone primary-response genes regulate common morphogenetic pathways during insect metamorphosis...
  73. pmc Genomic regions required for morphogenesis of the Drosophila embryonic midgut
    D Bilder
    Department of Developmental Biology, Stanford University School of Medicine, California 94305 5427, USA
    Genetics 141:1087-100. 1995
    ..Nine regions are required for proper formation of the midgut constrictions. The E75 ecdysone-induced gene, which encodes a nuclear receptor superfamily member, is the relevant gene in one region and ..
  74. ncbi Drosophila TFIID binds to a conserved downstream basal promoter element that is present in many TATA-box-deficient promoters
    T W Burke
    Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla, 92093 0347, USA
    Genes Dev 10:711-24. 1996
    ..These results suggest that the DPE acts in conjunction with the initiation site sequence to provide a binding site for TFIID in the absence of a TATA box to mediate transcription of TATA-less promoters...
  75. pmc The Drosophila Eip78C gene is not vital but has a role in regulating chromosome puffs
    S R Russell
    Department of Genetics, University of Cambridge, United Kingdom
    Genetics 144:159-70. 1996
    ..These data indicate either that Eip78C function is redundant or that it plays a subtle modulating role in the regulation of chromosome puffing...
  76. ncbi Ecdysone and insect immunity: the maturation of the inducibility of the diptericin gene in Drosophila larvae
    M Meister
    UPR 9022, I B M C, Strasbourg, France
    Insect Biochem Mol Biol 26:155-60. 1996
    ..However, experiments with the late larval lethal Broad-Complex mutant l(1)t435 show that the regulation of this response is distinct from the developmental ecdysone regulated hierarchies...
  77. ncbi Ecdysone signaling cascade and regulation of Drosophila metamorphosis
    E H Baehrecke
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park 20742, USA
    Arch Insect Biochem Physiol 33:231-44. 1996
    ..The regulation and function of these genes is discussed in the context of the cell- and tissue-specific changes required for the reorganization of a larva to form an adult fly...
  78. ncbi Coordination of Drosophila metamorphosis by two ecdysone-induced nuclear receptors
    K P White
    Department of Developmental Biology, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305 5427, USA
    Science 276:114-7. 1997
    The functions of the ecdysone-induced DHR3 and E75B orphan nuclear receptors in the early stages of Drosophila metamorphosis were investigated...
  79. ncbi Coordination of larval and prepupal gene expression by the DHR3 orphan receptor during Drosophila metamorphosis
    G T Lam
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    Development 124:1757-69. 1997
    ..DHR3 is sufficient to repress BR-C, E74A, E75A and E78B transcription as well as induce betaFTZ-F1...
  80. ncbi Dueling orphans--interacting nuclear receptors coordinate Drosophila metamorphosis
    C S Thummel
    Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, USA
    Bioessays 19:669-72. 1997
    ..A recent paper provides functions for two of these receptors, E75B and DHR3, through trans-regulation and heterodimerization...
  81. ncbi crooked legs encodes a family of zinc finger proteins required for leg morphogenesis and ecdysone-regulated gene expression during Drosophila metamorphosis
    P P D'Avino
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah, Salt Lake City, Utah 84112 5331, USA
    Development 125:1733-45. 1998
    ..The EcR ecdysone receptor, and the BR-C, E74 and E75 early regulatory genes, are submaximally induced in crol mutants in response to the prepupal ecdysone pulse...
  82. ncbi Structure of the Drosophila DNA topoisomerase I gene and expression of messages with different lengths in the 3' untranslated region
    S D Brown
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Gene 211:195-203. 1998
    ..No significant heterogeneity at the 5' end of the top1 transcripts is seen. Sequence searches have revealed a number of regulatory sequences for potential translational control in the 3' untranslated region...
  83. ncbi Drosophila metamorphosis: the only way is USP?
    M Buszczak
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA
    Curr Biol 8:R879-82. 1998
    ..Recent findings suggest that other hormones and receptors might also be involved...
  84. ncbi The acquisition of competence to respond to ecdysone in Drosophila is transcript specific
    G Richards
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, BP 163 67404 Illkirch Cedex, Strasbourg, France
    Mech Dev 82:131-9. 1999
    ..Furthermore these inhibitors lead to the induction of betaFTZ-F1 and E93 transcripts at levels normally found in prepupal glands. These studies reveal the complexity of the processes underlying the establishment of a hormonal response...
  85. ncbi The one-to-four rule and paralogues of sex-determining genes
    S Ohno
    Beckman Research Institute of the City of Hope, Duarte, California 91010 3000, USA
    Cell Mol Life Sci 55:824-30. 1999
    ..It is likely that one of them plays a pivotal role in the estrogen-dependent sex-determining mechanism so commonly found among reptiles, amphibians and fish...
  86. ncbi DHR3 is required for the prepupal-pupal transition and differentiation of adult structures during Drosophila metamorphosis
    G Lam
    Howard Hughes Medical Institute, University of Utah, 15 N 2030 E Rm 5100, Salt Lake City, Utah, 84112 5331, USA
    Dev Biol 212:204-16. 1999
    ....
  87. pmc The E23 early gene of Drosophila encodes an ecdysone-inducible ATP-binding cassette transporter capable of repressing ecdysone-mediated gene activation
    T Hock
    Department of Biological Science and Program in Medical Sciences, Florida State University, Tallahassee 32306 4370, USA
    Proc Natl Acad Sci U S A 97:9519-24. 2000
    ..Our results suggest the existence of a previously unrecognized regulatory mechanism for modulating steroid hormone signaling in Drosophila...
  88. ncbi A modifier screen of ectopic Krüppel activity identifies autosomal Drosophila chromosomal sites and genes required for normal eye development
    S Abrell
    Abteilung Molekulare Entwicklungsbiologie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    Chromosoma 109:334-42. 2000
    ..Owing to the bias of the screening system applied, these modifier genes will be expressed and are likely to be required during Drosophila wild-type eye development...
  89. ncbi Steroid regulation of programmed cell death during Drosophila development
    E H Baehrecke
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, Maryland, MD 20742, USA
    Cell Death Differ 7:1057-62. 2000
    ..This article reviews the current knowledge of steroid signaling and the regulation of programmed cell death during development of Drosophila...
  90. ncbi Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila
    T Lee
    Department of Biological Sciences, Stanford University, Stanford, CA 94305
    Neuron 28:807-18. 2000
    ..Surprisingly, most identified primary EcR/USP targets are dispensable for MB neuronal remodeling. Our study demonstrates cell-autonomous roles for EcR/USP in controlling neuronal remodeling, potentially through novel downstream targets...
  91. ncbi Sequence and functional properties of Ets genes in the model organism Drosophila
    T Hsu
    Center for Molecular and Structural Biology, Hollings Cancer Center, and Department of Cell Biology and Anatomy, Medical University of South Carolina, 86 Jonathan Lucas Street, Charleston, South Carolina, SC 29425, USA
    Oncogene 19:6409-16. 2000
    ..The information should be of significant value to the study of related processes in higher organisms due to the growing evidence for the cross species conservation of developmental mechanisms...
  92. ncbi Androgen-dependent neurodegeneration by polyglutamine-expanded human androgen receptor in Drosophila
    Ken ichi Takeyama
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo, Japan
    Neuron 35:855-64. 2002
    ..Thus, our findings suggest that the full binding of androgen to the polyQ-expanded hAR mutants leads to structural alteration with nuclear translocation that eventually results in the onset of SBMA in male patients...
  93. ncbi Isoform specific control of gene activity in vivo by the Drosophila ecdysone receptor
    Margrit Schubiger
    Department of Biology, University of Washington, Seattle WA 98195, USA
    Mech Dev 120:909-18. 2003
    ..EcR-B1 or EcR-B2 over-expression had little or no effect...
  94. ncbi Juvenile hormone regulation of the E75 nuclear receptor is conserved in Diptera and Lepidoptera
    Veronica A Dubrovskaya
    Department of Biology, Dartmouth College, Hanover, NH 03755, USA
    Gene 340:171-7. 2004
    ..In Drosophila melanogaster (Diptera), JH activates expression of the E75A nuclear receptor. The E75 gene encodes a family of related proteins...
  95. ncbi Ecdysone-mediated up-regulation of the effector caspase DRICE is required for hormone-dependent apoptosis in Drosophila cells
    Zoé E Kilpatrick
    Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA 5000, Australia
    J Biol Chem 280:11981-6. 2005
    ..Combined with previous observations, our results indicate that transcriptional regulation of the components of the core apoptotic machinery plays a key role in hormone-regulated programmed cell death during Drosophila development...
  96. ncbi The Drosophila caspase DRONC is required for metamorphosis and cell death in response to irradiation and developmental signals
    Markus Waldhuber
    Laboratory for Molecular Biology, Gene Center, Ludwig Maximilian University Munich, Feodor Lynen Strasse 25, 81377, Munich, Germany
    Mech Dev 122:914-27. 2005
    ..The levels of ecdysone-inducible transcripts such as E75A and Reaper (Rpr) are normal in the absence of DRONC, suggesting that DRONC acts downstream of these genes...
  97. ncbi The ecdysone-induced DHR4 orphan nuclear receptor coordinates growth and maturation in Drosophila
    Kirst King-Jones
    Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    Cell 121:773-84. 2005
    ..We propose that DHR4 coordinates growth and maturation in Drosophila by mediating endocrine responses to the attainment of critical weight during larval development...
  98. ncbi Powered by gas--a ligand for a fruit fly nuclear receptor
    Carl S Thummel
    Department of Human Genetics, Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City 84112, USA
    Cell 122:151-3. 2005
    ..In this issue of Cell, report that the fruit fly E75 nuclear receptor contains heme in its ligand binding pocket and that the oxidation state of this molecule controls E75 ..
  99. ncbi E75A and E75B have opposite effects on the apoptosis/development choice of the Drosophila egg chamber
    J Terashima
    Institute of Cell Biology, School of Biology, University of Edinburgh, Edinburgh EH9 3JR, UK
    Cell Death Differ 13:454-64. 2006
    ..We show that ecdysone response genes E75A and E75B are involved in inducing or suppressing apoptosis...
  100. ncbi Broad-Complex, E74, and E75 early genes control DNA puff BhC4-1 expression in prepupal salivary glands
    L R Basso
    Departamento de Biologia Celular e Molecular e de Bioagentes Patogênicos, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil
    Genesis 44:505-14. 2006
    ..expression, we utilized a candidate gene approach and tested the roles of the ecdysone-induced genes BR-C, E74, and E75. Our results reveal that the BR-C Z3 isoform is essential for BhC4-1-lacZ induction in prepupal salivary glands and ..

Research Grants2

  1. STEROID REGULATION OF PROGRAMMED CELL DEATH
    CARL THUMMEL; Fiscal Year: 2003
    ..In addition, this study will provide insight into the molecular mechanisms by which systemic hormonal signals are refined into stage- and tissue-specific biological responses during development. ..
  2. Mechanisms of Steroid-Triggered Programmed Cell Death
    CARL THUMMEL; Fiscal Year: 2009
    ..Finally, our studies provide a foundation for determining how steroids control cell death in humans, with implications for understanding and treating human disease. ..