dsx

Summary

Gene Symbol: dsx
Description: doublesex
Alias: CG11094, DSX, Dmdsx, Dmel\CG11094, Dsx, dsxF, dsxM, ix-62c, CG11094 gene product from transcript CG11094-RA, CG11094-PA, CG11094-PB, CG11094-PC, CG11094-PD, CG11094-PE, CG11094-PF, double sex, dsx-PA, dsx-PB, dsx-PC, dsx-PD, dsx-PE, dsx-PF, hermaphrodite, intersex-62c
Species: fruit fly

Top Publications

  1. pmc The Drosophila doublesex proteins share a novel zinc finger related DNA binding domain
    S E Erdman
    Department of Genetics, University of California, Davis 95616
    EMBO J 12:527-35. 1993
  2. ncbi The evolutionary dynamics of sex determination
    I Marin
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 281:1990-4. 1998
  3. ncbi Sex-determination gene and pathway evolution in nematodes
    Paul Stothard
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
    Bioessays 25:221-31. 2003
  4. pmc Fruitless, doublesex and the genetics of social behavior in Drosophila melanogaster
    Kathleen K Siwicki
    Department of Biology, Swarthmore College, 500 College Aveune, Swarthmore, PA 19081, USA
    Curr Opin Neurobiol 19:200-6. 2009
  5. pmc Midline crossing by gustatory receptor neuron axons is regulated by fruitless, doublesex and the Roundabout receptors
    David J Mellert
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Development 137:323-32. 2010
  6. pmc Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila
    Wei Wang
    Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487 0344, USA
    Proc Natl Acad Sci U S A 108:11139-44. 2011
  7. ncbi Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex
    Monika Hediger
    Zoological Institute, University Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Dev Genes Evol 214:29-42. 2004
  8. pmc Regulation of sex-specific selection of fruitless 5' splice sites by transformer and transformer-2
    V Heinrichs
    Department of Biological Sciences, Stanford University, California 94305 5020, USA
    Mol Cell Biol 18:450-8. 1998
  9. ncbi The mating of a fly
    J C Hall
    Department of Biology, Brandeis University, Waltham, MA 02254 9110
    Science 264:1702-14. 1994
  10. ncbi Sex-specific deployment of FGF signaling in Drosophila recruits mesodermal cells into the male genital imaginal disc
    Shaad M Ahmad
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 109:651-61. 2002

Research Grants

  1. NEURON DIFFERENTIATION IN C ELEGANS
    Scott Emmons; Fiscal Year: 2002
  2. AGE-RELATED NON-DISJUNCTION IN OOCYTES
    HELEN SALZ; Fiscal Year: 2004
  3. Molecular Neurobiology of Behavioral Rhythms
    Jeffrey Hall; Fiscal Year: 2006
  4. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
  5. Quantitative genomics of sexual dimorphism
    Sergey Nuzhdin; Fiscal Year: 2005
  6. Regulation of Sex-lethal pre-mRNA splicing during Drosophila development
    HELEN KAREN SALZ; Fiscal Year: 2010
  7. THERMODYNAMICS AND STRUCTURE OF POLYMERIZING ACTIN
    SANDRA GREER; Fiscal Year: 2001
  8. GENETIC ANALYSIS OF MORPHOGENESIS IN A NEMATODE
    Scott Emmons; Fiscal Year: 2005
  9. Identification of circadian clock output genes
    Mark Drapeau; Fiscal Year: 2006
  10. NEUROGENETICS OF DROSOPHILA REPRODUCTION
    LESLIE GRIFFITH; Fiscal Year: 2007

Scientific Experts

Detail Information

Publications141 found, 100 shown here

  1. pmc The Drosophila doublesex proteins share a novel zinc finger related DNA binding domain
    S E Erdman
    Department of Genetics, University of California, Davis 95616
    EMBO J 12:527-35. 1993
    ..We have further shown that of 10 null dsx alleles, six encode proteins deficient in DNA binding activity, and that three of these alleles are the result of ..
  2. ncbi The evolutionary dynamics of sex determination
    I Marin
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Science 281:1990-4. 1998
    ....
  3. ncbi Sex-determination gene and pathway evolution in nematodes
    Paul Stothard
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
    Bioessays 25:221-31. 2003
    ..Experimental strategies that hold promise for illuminating pathway differences between nematodes are also discussed...
  4. pmc Fruitless, doublesex and the genetics of social behavior in Drosophila melanogaster
    Kathleen K Siwicki
    Department of Biology, Swarthmore College, 500 College Aveune, Swarthmore, PA 19081, USA
    Curr Opin Neurobiol 19:200-6. 2009
    ..Several recent articles refute this notion, however, demonstrating that at a minimum, both fruitless and doublesex are involved in establishing sexually dimorphic features of neural circuitry and behavior in fruit flies...
  5. pmc Midline crossing by gustatory receptor neuron axons is regulated by fruitless, doublesex and the Roundabout receptors
    David J Mellert
    Department of Biology, Stanford University, Stanford, CA 94305, USA
    Development 137:323-32. 2010
    ..We show that the sex determination genes fruitless (fru) and doublesex (dsx) both contribute to establishing this sexual dimorphism...
  6. pmc Sexually dimorphic regulation of the Wingless morphogen controls sex-specific segment number in Drosophila
    Wei Wang
    Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487 0344, USA
    Proc Natl Acad Sci U S A 108:11139-44. 2011
    ..segment by the combination of the Hox protein Abdominal-B (Abd-B) and the sex-determination regulator Doublesex (Dsx)...
  7. ncbi Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex
    Monika Hediger
    Zoological Institute, University Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Dev Genes Evol 214:29-42. 2004
    ..Here we present evidence that they converge at the level of the terminal regulator, doublesex ( dsx), which conveys the selected sexual fate to the differentiation genes...
  8. pmc Regulation of sex-specific selection of fruitless 5' splice sites by transformer and transformer-2
    V Heinrichs
    Department of Biological Sciences, Stanford University, California 94305 5020, USA
    Mol Cell Biol 18:450-8. 1998
    ..sufficient to promote the activation by tra and tra-2 of both a 5' splice site and the female-specific doublesex (dsx) 3' splice site, suggesting that the mechanisms of 5' splice site activation and 3' splice site activation may be ..
  9. ncbi The mating of a fly
    J C Hall
    Department of Biology, Brandeis University, Waltham, MA 02254 9110
    Science 264:1702-14. 1994
    ..Further behavioral mutations seemed originally to be courtship-specific, turned out not to have that property, and have led to a broadened perspective on the nature and action of Drosophila's sex-determination genes...
  10. ncbi Sex-specific deployment of FGF signaling in Drosophila recruits mesodermal cells into the male genital imaginal disc
    Shaad M Ahmad
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 109:651-61. 2002
    ..The recruited mesodermal cells migrate into the disc late in development and are neither anterior nor posterior...
  11. doi The female-specific doublesex isoform regulates pleiotropic transcription factors to pattern genital development in Drosophila
    Sujash S Chatterjee
    Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA
    Development 138:1099-109. 2011
    ..In flies, the sex-determination hierarchy terminates in the doublesex (dsx) gene, which produces sex-specific transcription factors via alternative splicing of its transcripts...
  12. pmc Evolution of sex-specific traits through changes in HOX-dependent doublesex expression
    Kohtaro Tanaka
    Department of Evolution and Ecology, University of California Davis, Davis, California, United States of America
    PLoS Biol 9:e1001131. 2011
    ..We examine the regulation and function of doublesex (dsx), the main transcriptional effector of the sex determination pathway, in the development and evolution of ..
  13. doi Hox-mediated regulation of doublesex sculpts sex-specific abdomen morphology in Drosophila
    Wei Wang
    Department of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama 35487, USA
    Dev Dyn 241:1076-90. 2012
    ..regulation mediated by the Hox protein Abdominal-B (Abd-B) and products of the sex-determination gene doublesex (dsx)...
  14. ncbi The remarkable ubiquity of DM domain factors as regulators of sexual phenotype: ancestry or aptitude?
    Jonathan Hodgkin
    Genetics Unit, Department of Biochemistry, University of Oxford, OX1 3QU, UK
    Genes Dev 16:2322-6. 2002
  15. ncbi The Drosophila sex determination hierarchy modulates wingless and decapentaplegic signaling to deploy dachshund sex-specifically in the genital imaginal disc
    E L Keisman
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Development 128:1643-56. 2001
    ..The last known gene in the somatic branch of this hierarchy is the transcription factor doublesex (dsx); however, targets of the hierarchy that play a role in sexually dimorphic development have remained elusive...
  16. ncbi Evolution in black and white: genetic control of pigment patterns in Drosophila
    Patricia J Wittkopp
    Molecular Biology and Genetics, 227 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Trends Genet 19:495-504. 2003
  17. ncbi A putative Drosophila pheromone receptor expressed in male-specific taste neurons is required for efficient courtship
    Steven Bray
    Duke University Medical Center, Department of Molecular Genetics and Microbiology, 252 CARL Building, Research Drive, Durham, NC 27710, USA
    Neuron 39:1019-29. 2003
    ....
  18. ncbi Separate DNA sequences are required for normal female and ecdysone-induced male expression of Drosophila melanogaster yolk protein 1
    A D Shirras
    Department of Molecular Biology, University of Edinburgh, UK
    Mol Gen Genet 210:153-5. 1987
    ..ADH activity is not present in dsxD/dsx pseudomales indicating that this fragment contains sites where the dsx gene product exerts its effect...
  19. pmc The doublesex proteins of Drosophila melanogaster bind directly to a sex-specific yolk protein gene enhancer
    K C Burtis
    Department of Genetics, University of California, Davis 95616
    EMBO J 10:2577-82. 1991
    The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific and female-specific polypeptides, whose synthesis is regulated by alternative sex-specific splicing of the primary dsx transcript...
  20. pmc Binding of the Drosophila transformer and transformer-2 proteins to the regulatory elements of doublesex primary transcript for sex-specific RNA processing
    K Inoue
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Proc Natl Acad Sci U S A 89:8092-6. 1992
    Sex-specific alternative processing of double-sex (dsx) precursor messenger RNA (pre-mRNA) is one of the key steps that regulates somatic sexual differentiation in Drosophila melanogaster...
  21. ncbi Moving up the hierarchy: a hypothesis on the evolution of a genetic sex determination pathway
    A S Wilkins
    Company of Biologists, Ltd, New Museums Site, University of Cambridge, UK
    Bioessays 17:71-7. 1995
    ..Finally, the specific molecular and population genetic questions that the model raises are described and some tests are proposed...
  22. ncbi Are the same genes responsible for intra- and interspecific variability for sex comb tooth number in Drosophila?
    S V Nuzhdin
    Section of Evolution and Ecology, University of California at Davis, Davis, CA 95616, USA
    Heredity (Edinb) 84:97-102. 2000
    ..One intraspecific QTL mapped to the same interval as the QTL for interspecific differences between D. simulans and D. mauritiana. Whether or not these effects result from the same genes requires further examination...
  23. ncbi SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing
    Alan M Zahler
    Department of Molecular, Cellular and Developmental Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA
    J Biol Chem 279:10077-84. 2004
    ..We show that the SC35 and the hnRNP A1 binding sites overlap within the juxtaposed ESE/ESS. We propose that hnRNP A1 binding to the ESS inhibits splicing of the upstream intron by directly masking the SC35 binding site...
  24. pmc Somatic control of germline sexual development is mediated by the JAK/STAT pathway
    Matthew Wawersik
    Biology Department, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    Nature 436:563-7. 2005
    ..Our findings provide direct evidence that the JAK/STAT pathway mediates a key signal from the somatic gonad that regulates male germline sexual development...
  25. ncbi Commitment of abdominal neuroblasts in Drosophila to a male or female fate is dependent on genes of the sex-determining hierarchy
    B J Taylor
    Department of Zoology, University of Washington, Seattle 98195
    Development 114:625-42. 1992
    ..of the sexual pathway taken by sex-specific neuroblasts depends on the expression of one of these genes, doublesex (dsx)...
  26. pmc The doublesex locus of Drosophila melanogaster and its flanking regions: a cytogenetic analysis
    B S Baker
    Biology Department, University of California, San Diego 92093
    Genetics 127:125-38. 1991
    The region of the third chromosome (84D-F) of Drosophila melanogaster that contains the doublesex (dsx) locus has been cytogenetically analyzed...
  27. ncbi Regulation of doublesex pre-mRNA processing occurs by 3'-splice site activation
    L C Ryner
    Department of Biological Sciences, Stanford University, California 94305
    Genes Dev 5:2071-85. 1991
    Sex-specific alternative processing of the doublesex (dsx) pre-mRNA controls somatic sexual differentiation in Drosophila melanogaster...
  28. ncbi Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides
    K C Burtis
    Department of Biological Sciences, Stanford University, California 94305
    Cell 56:997-1010. 1989
    The doublesex (dsx) gene regulates somatic sexual differentiation in both sexes in D. melanogaster...
  29. ncbi Genetics of sex determination: what can we learn from Drosophila?
    R Nothiger
    Zoological Institute, University of Zurich, Switzerland
    Development 101:17-24. 1987
    ..The functional state, ON or OFF, of Sxl, via a few subordinate regulatory genes, controls a switch gene (dsx) that can express two mutually exclusive functions, M or F...
  30. ncbi Morphological analysis and fate map of the intersexual genital disc of the mutant doublesex-dominant in Drosophila melanogaster
    F Epper
    Dev Biol 88:104-14. 1981
  31. ncbi Autosomal mutations that interfere with sex determination in somatic cells of Drosophila have no direct effect on the germline
    T Schupbach
    Dev Biol 89:117-27. 1982
  32. pmc Three protein binding sites form an enhancer that regulates sex- and fat body-specific transcription of Drosophila yolk protein genes
    W An
    Department of Biochemistry, Brandeis University, Waltham, MA 02254 9110, USA
    EMBO J 14:1221-30. 1995
    ..A gene at the bottom of this pathway, doublesex, encodes the female-specific DSXF and male-specific DSXM proteins that bind to and regulate transcription from several sites in the Yp genes...
  33. ncbi A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
    M Tian
    Harvard University, Department of Biochemistry and Molecular Biology, Cambridge, Massachusetts 02138
    Cell 74:105-14. 1993
    Female-specific splicing of Drosophila doublesex (dsx) pre-mRNA is regulated by the products of the transformer (tra) and transformer 2 (tra2) genes...
  34. ncbi Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila
    K T Coschigano
    Department of Biochemistry, Brandeis University, Waltham Massachusetts 02254 9110
    Genes Dev 7:42-54. 1993
    The somatic sexual phenotype of Drosophila is regulated by the sexual differentiation pathway. Male (DSXM) and female (DSXF) proteins encoded by doublesex (dsx), a gene at the end of this pathway, bind to three sites within a 127-bp ..
  35. pmc Courtship anomalies caused by doublesex mutations in Drosophila melanogaster
    A Villella
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Genetics 143:331-44. 1996
    The role played by the sex-determining gene doublesex (dsx) and its influence on Drosophila courtship were examined...
  36. ncbi DNA binding by the male and female doublesex proteins of Drosophila melanogaster
    S Cho
    Rosenstiel Center and the Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    J Biol Chem 272:3185-9. 1997
    Drosophila yolk protein genes are regulated by doublesex male protein (DSXM) in males and doublesex female protein (DSXF) in females. Both proteins bind to the same DNA sites from which DSXM represses and DSXF activates transcription...
  37. pmc Reproductive isolation and morphogenetic evolution in Drosophila analyzed by breakage of ethological barriers
    L Sanchez
    Centro de Investigaciones Biologicas, Velazquez, Madrid, Spain
    Genetics 147:231-42. 1997
    ..The effect on the terminalia of teissieri-melanogaster hybrids of a mutation in doublesex, a regulatory gene that controls the development of the terminalia, was also investigated...
  38. pmc Analysis of the functional specificity of RS domains in vivo
    B Dauwalder
    Department of Molecular Genetics, University of Texas, MD Anderson Cancer Center, 1515 Holcombe Blvd, Box 45, Houston, TX 77030, USA
    EMBO J 17:6049-60. 1998
    ..These results indicate that RS domains are not all functionally equivalent in vivo...
  39. pmc Analysis of the doublesex female protein in Drosophila melanogaster: role on sexual differentiation and behavior and dependence on intersex
    J A Waterbury
    Department of Molecular Biology, Princeton University, Princeton, New Jersey, 08544, USA
    Genetics 152:1653-67. 1999
    doublesex (dsx) is unusual among the known sex-determination genes of Drosophila melanogaster in that functional homologs are found in distantly related species...
  40. ncbi intersex, a gene required for female sexual development in Drosophila, is expressed in both sexes and functions together with doublesex to regulate terminal differentiation
    Carrie M Garrett-Engele
    Department of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Development 129:4661-75. 2002
    ..domain, leads to the inference that ix may require the female-specific protein product of the doublesex (dsx) gene in order to function...
  41. pmc The evolution of the Drosophila sex-determination pathway
    Andrew Pomiankowski
    Department of Biology, University College, London NW1 2HE, United Kingdom
    Genetics 166:1761-73. 2004
    ..Our hypothesis is built on the available data from Drosophila and other insect species, and we point out where it is amenable to further experimental and comparative tests...
  42. ncbi Control of male sexual behavior in Drosophila by the sex determination pathway
    Jean Christophe Billeter
    IBLS Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK
    Curr Biol 16:R766-76. 2006
    ....
  43. ncbi Positive control of pre-mRNA splicing in vitro
    M Tian
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138
    Science 256:237-40. 1992
    Positive control of the sex-specific alternative splicing of doublesex (dsx) precursor messenger RNA (pre-mRNA) in Drosophila melanogaster involves the activation of a female-specific 3' splice site by the products of the transformer (..
  44. ncbi Sex differentiation: the role of alternative splicing
    M McKeown
    Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92186
    Curr Opin Genet Dev 2:299-303. 1992
    ..The results of recent work have verified much of the earlier molecular and genetic work in this field and have provided a demonstration that both positive and negative regulatory mechanisms are involved...
  45. ncbi Sex-specific splicing and polyadenylation of dsx pre-mRNA requires a sequence that binds specifically to tra-2 protein in vitro
    M L Hedley
    Harvard University, Department of Biochemistry and Molecular Biology, Cambridge, Massachusetts 02138
    Cell 65:579-86. 1991
    ..Female-specific splicing and/or polyadenylation of doublesex (dsx) pre-mRNA, the final gene in this pathway, requires transformer (tra) and transformer-2 (tra-2) proteins...
  46. ncbi Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila
    K Hoshijima
    Department of Biophysics, Faculty of Science, Kyoto University, Japan
    Science 252:833-6. 1991
    Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster...
  47. ncbi Regulation of sex-specific RNA splicing at the Drosophila doublesex gene: cis-acting mutations in exon sequences alter sex-specific RNA splicing patterns
    R N Nagoshi
    Department of Biological Sciences, Stanford University, California 94305 5020
    Genes Dev 4:89-97. 1990
    Sex-specific alternative RNA splicing of the doublesex (dsx) pre-mRNA results in sex-specific polypeptides that regulate both male and female somatic sexual differentiation in Drosophila melanogaster...
  48. ncbi A molecular analysis of doublesex, a bifunctional gene that controls both male and female sexual differentiation in Drosophila melanogaster
    B S Baker
    Department of Biology, University of California, San Diego, La Jolla 92093
    Genes Dev 2:477-89. 1988
    The doublesex (dsx) gene regulates somatic sexual differentiation in both sexes in Drosophila melanogaster. dsx has active but opposite negative regulatory functions in males and females...
  49. ncbi Ectopic expression of the female transformer gene product leads to female differentiation of chromosomally male Drosophila
    M McKeown
    Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138
    Cell 53:887-95. 1988
    ..Expression of the non-sex-specific tra RNA in tra mutants is not sufficient to lead to female differentiation. The tra female-specific activity is not required for female-specific splicing of the tra precursor RNAs...
  50. ncbi Sex determination in the germ line of Drosophila depends on genetic signals and inductive somatic factors
    R Nothiger
    Zoological Institute, University of Zurich, Switzerland
    Development 107:505-18. 1989
    ..by mutations at the sex-determining genes Sxl (Sex-lethal), tra (transformer), tra-2 (transformer-2), or dsx (double-sex)...
  51. ncbi Sex determination and dosage compensation in Drosophila melanogaster
    B S Baker
    Annu Rev Genet 17:345-93. 1983
  52. pmc Sex and the single cell. I. On the action of major loci affecting sex determination in Drosophila melanogaster
    B S Baker
    Genetics 94:383-423. 1980
    ..ratio and at least four major regulatory genes: transformer (tra), transformer-2 (tra-2), doublesex (dsx) and intersex (ix). Attention is focused here on the roles of these four loci in sex determination...
  53. pmc A genetic analysis of intersex, a gene regulating sexual differentiation in Drosophila melanogaster females
    B A Chase
    Department of Biological Sciences, Stanford University, California 94305, USA
    Genetics 139:1649-61. 1995
    ..Taken together with previous analyses, our results indicate that the ix+ product is required to function with the female-specific product of doublesex to implement appropriate female sexual differentiation in diplo-X animals...
  54. ncbi Integrating sex- and tissue-specific regulation within a single Drosophila enhancer
    W An
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254 9110
    Genes Dev 9:256-66. 1995
    ..It consists of four protein-binding sites: dsxA, which binds male (DSXM) and female (DSXF) proteins encoded by the doublesex gene; aef1, which binds the AEF1 repressor; bzip1, which binds the DmC/EBP ..
  55. ncbi A splicing enhancer exhibits both constitutive and regulated activities
    M Tian
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Genes Dev 8:1703-12. 1994
    ..Transformer (Tra) and Transformer2 (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) ..
  56. ncbi The regulation of the yolk protein genes, a family of sex differentiation genes in Drosophila melanogaster
    M Bownes
    Division of Biological Sciences, University of Edinburgh, UK
    Bioessays 16:745-52. 1994
    ....
  57. ncbi Behavioral and neurobiological implications of sex-determining factors in Drosophila
    B J Taylor
    Department of Zoology, Oregon State University, Corvallis
    Dev Genet 15:275-96. 1994
    ....
  58. ncbi The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2
    H Amrein
    Howard Hughes Medical Institute, Columbia University, New York, New York 10032
    Cell 76:735-46. 1994
    ..These results demonstrate that both protein-RNA and protein-protein interactions are involved in tra2-dependent activation and repression of alternative splicing...
  59. ncbi Sex-lethal, master and slave: a hierarchy of germ-line sex determination in Drosophila
    B Oliver
    Department of Biological Sciences, Stanford University, CA 94305
    Development 119:897-908. 1993
    ..The fused+, ovarian tumor+, ovo+ and sans fille+ genes function upstream of Sex-lethal+ in the germ line...
  60. ncbi A positive role in differentiation for the male doublesex protein of Drosophila
    V A Jursnich
    Department of Genetics, University of California, Davis 95616
    Dev Biol 155:235-49. 1993
    The doublesex (dsx) locus encodes male-specific and female-specific polypeptides that are essential for the proper differentiation of sexually dimorphic somatic features of Drosophila melanogaster...
  61. pmc Sex-specific and non-sex-specific oligomerization domains in both of the doublesex transcription factors from Drosophila melanogaster
    W An
    Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Mol Cell Biol 16:3106-11. 1996
    ..gene of Drosophila melanogaster encodes the alternatively spliced, sex-specific transcription factors DSXM and DSXF. These factors regulate male- and female-specific transcription of many genes...
  62. ncbi Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer
    K W Lynch
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 10:2089-101. 1996
    ..These observations show that the protein composition of each complex is determined by the RNA recognition sequence and specific interactions between SR proteins and Tra and Tra2...
  63. pmc Functional and genetic characterization of the oligomerization and DNA binding properties of the Drosophila doublesex proteins
    S E Erdman
    Section of Molecular and Cellular Biology, University of California, Davis 95616, USA
    Genetics 144:1639-52. 1996
    The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific (DSXM) and female-specific (DSXF) polypeptides, which are required for normal differentiation of numerous sexually dimorphic somatic traits...
  64. ncbi Vive la différence: males vs females in flies vs worms
    T W Cline
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3204, USA
    Annu Rev Genet 30:637-702. 1996
    ..Although no overlap has been found among the molecules used by flies and worms to achieve sex determination, striking similarities have been found in the genetic strategies used by these two species to differentiate their sexes...
  65. ncbi Genetic feminization of pheromones and its behavioral consequences in Drosophila males
    J F Ferveur
    Mecanismes de Communication, Unité de Recherche Associée CNRS 1491, Batiment 446, Universite Paris Sud, 91405, Orsay Cedex, France
    Science 276:1555-8. 1997
    ..The resulting flies generally exhibited male heterosexual orientation but elicited homosexual courtship from other males...
  66. ncbi Evidence for evolutionary conservation of sex-determining genes
    C S Raymond
    Institute of Human Genetics, Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455, USA
    Nature 391:691-5. 1998
    ..elegans and found that it is related to the Drosophila melanogaster sexual regulatory gene doublesex (dsx)2. Both genes encode proteins with a DNA-binding motif that we have named the 'DM domain'...
  67. ncbi hermaphrodite and doublesex function both dependently and independently to control various aspects of sexual differentiation in Drosophila
    H Li
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Development 125:2641-51. 1998
    The hermaphrodite (her) gene is necessary for sexual differentiation in Drosophila...
  68. ncbi The function of multisite splicing enhancers
    K J Hertel
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Mol Cell 1:449-55. 1998
    ..Thus, the function of multisite enhancer elements to increase the probability of an interaction between the enhancer complex and the splicing machinery rather than to promote functional synergy...
  69. ncbi Alternative splicing of pre-mRNA: developmental consequences and mechanisms of regulation
    A J Lopez
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Annu Rev Genet 32:279-305. 1998
    ..Cooperative and antagonistic effects integrated at regulatory elements are likely to be important for specificity and for finely tuned differences in cell-type-specific alternative splicing patterns...
  70. ncbi Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases
    B J Blencowe
    Dept of Medical Research, C H Best Institute, University of Toronto, 112 College St, Toronto, Ontario, Canada M5G 1L6
    Trends Biochem Sci 25:106-10. 2000
    ..Ominously, these latter studies predict that many human genetic diseases linked to mutations within exons might be caused by the inactivation of ESEs...
  71. ncbi Genetic control and evolution of sexually dimorphic characters in Drosophila
    A Kopp
    Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin Madison, 53706 1596, USA
    Nature 408:553-9. 2000
    ..Pigmentation patterns specified by bab affect mating preferences, suggesting that sexual selection has contributed to the evolution of bab regulation...
  72. ncbi Sex determination genes control the development of the Drosophila genital disc, modulating the response to Hedgehog, Wingless and Decapentaplegic signals
    L Sanchez
    Centro de Investigaciones Biologicas, C S I C, Velazquez 144, Madrid, Spain
    Development 128:1033-43. 2001
    ..A model is presented for the dimorphic sexual development of the genital primordium in which both Doublesex(M) and Doublesex(F) products play positive and negative roles...
  73. ncbi Are complex behaviors specified by dedicated regulatory genes? Reasoning from Drosophila
    B S Baker
    Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Cell 105:13-24. 2001
  74. ncbi The development of the Drosophila genital disc
    L Sanchez
    Centro de Investigaciones Biologicas, CSIC, Madrid, Spain
    Bioessays 23:698-707. 2001
    ..This input acts by modulating the response to Hedgehog, Wingless, and Decapentaplegic morphogenetic signals...
  75. ncbi Doublesex surprises
    S Vincent
    Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 106:399-402. 2001
  76. ncbi Development. Doublesex in the middle
    Kenneth C Burtis
    Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Science 297:1135-6. 2002
  77. ncbi Sex comes in from the cold: the integration of sex and pattern
    Audrey E Christiansen
    Department Biological Sciences, Stanford University, Stanford, CA 94305, USA
    Trends Genet 18:510-6. 2002
    ....
  78. pmc The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior
    Brigitte Dauwalder
    Department of Molecular Genetics, University of Texas, M D Anderson Cancer Center, Houston, Texas 77030, USA
    Genes Dev 16:2879-92. 2002
    ..fat body tissue closely associated with the adult brain and are dependent on the sex determination genes doublesex (dsx) and fruitless (fru)...
  79. ncbi Masters change, slaves remain
    Patricia Graham
    Dept Molecular Biology, Princeton University, NJ 08544 1041, USA
    Bioessays 25:1-4. 2003
    ..of a downstream gene, transformer (tra), which acts with tra2 to control alternative splicing of doublesex (dsx)...
  80. ncbi Doublesex gene expression in the central nervous system of Drosophila melanogaster
    Gyunghee Lee
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurogenet 16:229-48. 2002
    Despite several behavior-genetic studies that have suggested roles played by doublesex (dsx) in neural tissues, it has not been demonstrated that the products of this gene are actually present in the central nervous system (CNS)...
  81. pmc Direct repression of splicing by transformer-2
    Dawn S Chandler
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030 4009, USA
    Mol Cell Biol 23:5174-85. 2003
    The Drosophila melanogaster sex determination factor Tra2 positively regulates the splicing of both doublesex (dsx) and fruitless (fru) pre-mRNAs but negatively affects the splicing of the M1 intron in tra2 pre-mRNA...
  82. ncbi Sex-specific apoptosis regulates sexual dimorphism in the Drosophila embryonic gonad
    Tony J DeFalco
    Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
    Dev Cell 5:205-16. 2003
    ..Our work furthers the hypotheses that a conserved pathway controls gonad sexual dimorphism in diverse species and that sex-specific cell recruitment and programmed cell death are common mechanisms for creating sexual dimorphism...
  83. ncbi Genetic variation for expression of the sex determination pathway genes in Drosophila melanogaster
    Aaron M Tarone
    Evolution and Ecology, University of California at Davis, CA 95616, USA
    Genet Res 86:31-40. 2005
    ..Expression of Sex lethal, transformer, transformer2, doublesex, intersex and hermaphrodite was assayed with quantitative RT-PCR in 0- to 1-day-old adult males and females of 36 Drosophila melanogaster ..
  84. ncbi Isoform-specific control of male neuronal differentiation and behavior in Drosophila by the fruitless gene
    Jean Christophe Billeter
    IBLS Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, United Kingdom
    Curr Biol 16:1063-76. 2006
    ..These putative transcription factors, containing one of three alternative DNA binding domains, determine the neuronal substrates for sexual behavior in male CNS...
  85. ncbi The control of sexual identity in the Drosophila germline
    Abbie Casper
    Department of Biology, 302 Mudd Hall, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Development 133:2783-91. 2006
    ..Here, we explore how sexual identity is established in the Drosophila germline, how this affects other aspects of germ cell development and what studies in Drosophila can teach us about mammalian germ cells...
  86. ncbi A double-switch system regulates male courtship behavior in male and female Drosophila melanogaster
    Troy R Shirangi
    Molecular Biology, Cell Biology, and Biochemistry Department, Brown University, 69 Brown Street, Providence, Rhode Island 02912, USA
    Nat Genet 38:1435-9. 2006
    ..In males, consistent with the opposing functions of dsxM and dsxF, dsxM acts as a positive factor for male courtship...
  87. pmc The sex-determination genes fruitless and doublesex specify a neural substrate required for courtship song
    Elizabeth J Rideout
    Division of Molecular Genetics, University of Glasgow, Anderson College, Glasgow, UK
    Curr Biol 17:1473-8. 2007
    ..ganglion (Msg), revealed the coexpression of two sex-determination genes, fruitless (fru) and doublesex (dsx)...
  88. pmc Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells
    Leonie U Hempel
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, 50 South Drive, Bethesda MD 20892 USA
    BMC Dev Biol 7:113. 2007
    ..The male-specific DSXM represses genes involved in female development and activates genes involved in male development...
  89. pmc Doublesex establishes sexual dimorphism in the Drosophila central nervous system in an isoform-dependent manner by directing cell number
    Laura E Sanders
    Sections of Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA
    Dev Biol 320:378-90. 2008
    doublesex (dsx) encodes sex-specific transcription factors (DSX(F) in females and DSX(M) in males) that act at the bottom of the Drosophila somatic sex determination hierarchy...
  90. pmc The regulation and evolution of a genetic switch controlling sexually dimorphic traits in Drosophila
    Thomas M Williams
    Howard Hughes Medical Institute and Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Cell 134:610-23. 2008
    ..We show that the HOX protein Abdominal-B (ABD-B) and the sex-specific isoforms of Doublesex (DSX) directly regulate a bab cis-regulatory element (CRE)...
  91. doi Fruitless and doublesex coordinate to generate male-specific neurons that can initiate courtship
    Ken ichi Kimura
    Laboratory of Biology, Iwamizawa Campus, Hokkaido University of Education, Iwamizawa 068 8642, Japan
    Neuron 59:759-69. 2008
    ..P1 is fated to die in females through the action of a feminizing protein, DsxF. A masculinizing protein Fru is required in the male brain for correct positioning of the terminals of P1 neurites...
  92. pmc Rapid evolution of sex pheromone-producing enzyme expression in Drosophila
    Troy R Shirangi
    Howard Hughes Medical Institute, University of Wisconsin, Madison, United States of America
    PLoS Biol 7:e1000168. 2009
    ....
  93. doi Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila
    Maria Fernanda Ruiz
    Centro de Investigaciones Biologicas CSIC, Madrid, Spain
    Int J Dev Biol 54:627-33. 2010
    ..The Anastrepha Tra protein induced the female-specific splicing of both dsx and fru pre-mRNAs in Drosophila XY male flies, so that these became transformed into females, though this ..
  94. pmc Control of sexual differentiation and behavior by the doublesex gene in Drosophila melanogaster
    Elizabeth J Rideout
    Faculty of Biomedical and Life Sciences, Integrative and Systems Biology, University of Glasgow, Glasgow, UK
    Nat Neurosci 13:458-66. 2010
    ..We inserted Gal4 into the doublesex (dsx) locus of Drosophila melanogaster, allowing us to visualize and manipulate cells expressing dsx in various tissues...
  95. pmc Sexual dimorphism in the fly brain
    Sebastian Cachero
    Division of Neurobiology, MRC Laboratory of Molecular Biology, Cambridge, UK
    Curr Biol 20:1589-601. 2010
    ..Subsequently, three discrete anatomical differences in central brain fru+ neurons have been reported, but the global organization of sex differences in wiring is unclear...
  96. pmc Function and evolution of sex determination mechanisms, genes and pathways in insects
    Tanja Gempe
    Department of Genetics, Heinrich Heine University, Duesseldorf, Universitaetsstrasse 1, Duesseldorf, Germany
    Bioessays 33:52-60. 2011
    ..This review summarises recent findings in insects, surveys evidence of how and why sex determination mechanisms can change rapidly and suggests fruitful areas of future research...
  97. pmc Direct targets of the D. melanogaster DSXF protein and the evolution of sexual development
    Shengzhan D Luo
    Biology Department, Stanford University, Stanford, CA 94305, USA
    Development 138:2761-71. 2011
    Uncovering the direct regulatory targets of doublesex (dsx) and fruitless (fru) is crucial for an understanding of how they regulate sexual development, morphogenesis, differentiation and adult functions (including behavior) in ..
  98. pmc Turning males on: activation of male courtship behavior in Drosophila melanogaster
    Yufeng Pan
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America
    PLoS ONE 6:e21144. 2011
    ..The potential for male sexual behavior in D. melanogaster is specified by the fruitless (fru) and doublesex (dsx) sex regulatory genes...
  99. doi The roles of fruitless and doublesex in the control of male courtship
    Brigitte Dauwalder
    University of Houston, Department of Biology and Biochemistry, 369 SR2, Houston, TX 77204 5001, USA
    Int Rev Neurobiol 99:87-105. 2011
    ..In particular it discusses how doublesex and fruitless contribute to the generation of sexually dimorphic neurons, the role of cell death, and the emerging information about circuits that underlie the behavior...
  100. pmc Substrate-borne vibratory communication during courtship in Drosophila melanogaster
    Caroline C G Fabre
    Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 2EJ, UK
    Curr Biol 22:2180-5. 2012
    ..Our findings in Drosophila open a door to study the neuromuscular circuitry responsible for these signals and the sensory systems needed for their reception...

Research Grants12

  1. NEURON DIFFERENTIATION IN C ELEGANS
    Scott Emmons; Fiscal Year: 2002
    ..3) screen for mutants (including ts alleles) in which TH::GFP expression is lost and characterize the corresponding genes molecularly. (4) identify genes involved in the axon connectivity of DA neurons. ..
  2. AGE-RELATED NON-DISJUNCTION IN OOCYTES
    HELEN SALZ; Fiscal Year: 2004
    ..abstract_text> ..
  3. Molecular Neurobiology of Behavioral Rhythms
    Jeffrey Hall; Fiscal Year: 2006
    ..of chronobiology in this model system may provide insights into the nature of certain rhythm-related disorders of humans, some of which are associated with genetic variations of Drosophila clock gene orthologs [unreadable] [unreadable]..
  4. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
    ..Thus, we exploit the powerful genetics of Drosophila to elucidate efficiently the molecules and gene pathways needed to start development in humans. ..
  5. Quantitative genomics of sexual dimorphism
    Sergey Nuzhdin; Fiscal Year: 2005
    ..We expect our results to have a profound impact on our understanding of the evolution of sexual dimorphism, and the role that sexual differentiation plays in the generation of phenotypic diversity. ..
  6. Regulation of Sex-lethal pre-mRNA splicing during Drosophila development
    HELEN KAREN SALZ; Fiscal Year: 2010
    ..Due to the remarkable conservation of the splicing machinery from humans to flies, the paradigms we develop using the very tractable Drosophila system are relevant to human biology. ..
  7. THERMODYNAMICS AND STRUCTURE OF POLYMERIZING ACTIN
    SANDRA GREER; Fiscal Year: 2001
    ..abstract_text> ..
  8. GENETIC ANALYSIS OF MORPHOGENESIS IN A NEMATODE
    Scott Emmons; Fiscal Year: 2005
    ..This research will contribute to understanding a fundamental aspect of animal development, and thus help towards progress in overcoming diseases that arise as a result of developmental abnormalities. ..
  9. Identification of circadian clock output genes
    Mark Drapeau; Fiscal Year: 2006
    ..Then, some of these genes will be studied molecularly to determine how they are regulated by the central clock. ..
  10. NEUROGENETICS OF DROSOPHILA REPRODUCTION
    LESLIE GRIFFITH; Fiscal Year: 2007
    ..function within the sex-determination hierarchy (SDH) of Drosophila melanogaster: fruitless (fru) and doublesex (dsx)...
  11. The Mechanisms and Regulatory Networks of Alternative Splicing in Drosophila
    BRENTON GRAVELEY; Fiscal Year: 2009
    ....
  12. Connectivity in the Nematode Nervous System
    Scott Emmons; Fiscal Year: 2002
    ..The preanal ganglion circuitry provides a unique opportunity to explore the development and function of a defined set of synapses during postembryonic life in a system which is not essential for survival in this hermaphroditic species. ..